Hematology profile of Rattus norvegicus infected by enteropathogenic Escherichia coli and fed probiotics

Versi online:
h p://medpet.journal.ipb.ac.id/
DOI: 10.5398/medpet.2011.34.1.7

Media Peternakan, April 2011, hlm. 7-13
EISSN 2087-4634
Terakreditasi B SK Dikti No: 43/DIKTI/Kep/2008

Gambaran Hematologi Tikus Putih (Ra us norvegicus) yang Diinfeksi
Escherichia coli Enteropatogenik dan Diberikan Probiotik
Hematology Profile of Ra us norvegicus Infected by Enteropathogenic
Escherichia coli and Fed Probiotics

M. Astawana, *, T. Wresdiyatib, #, I. I. Ariefc, #, & E. Suhestia
Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor
Jln. Kamper, Kampus IPB Darmaga Bogor 16680
b
Departemen Anatomi, Fisiologi dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
c
Departemen Ilmu Produksi dan Teknologi Peternakan, Fakultas Peternakan, Institut Pertanian Bogor
#

Jln. Agatis, Kampus IPB Darmaga Bogor 16680
(Diterima 07-06-2010; disetujui 10-03-2011)
a

ABSTRACT
In Indonesia, diarrhea is the second leading cause of death in children after ISPA (Acute
Respiratory Infection). This study aimed to test the ability of probiotic, Lactobacillus plantarum 2C12
and Lactobacillus acidophilus 2B4, as an indigenous anti-diarrhea in rats exposed to Enteropathogenic
Escherichia coli (EPEC) and find out their impact on erythrocytes, hematocrit, hemoglobin, thrombocytes, and leukocytes. A total of 95 male rats Sparague Dawley strain were alocated into six treatment groups: (1) negative control, (2) L. plantarum 2C12, (3) L. acidophilus 2B4, (4) L. plantarum 2C12
+ EPEC, (5) L. acidophilus 2B4 + EPEC, and (6) positive control (exposed to EPEC). Probiotics were
given from day 1 up to 21, whereas 106 cfu/ml EPEC was administered orally from day 8 to 14. On day
21, the number of erythrocytes, hematocrit and hemoglobin were the lowest (7.07 million/µl; 39.13%;
14.50 g/dl) in the positive group of rats. The positive control group had lower number of thrombocytes (389 thousand/ µl) (P