Performances and Meat Cholesterol Content of Fat Tail Sheep Fed Diets Supplemented with Sardinella Fish Oil Based Ca-soap Mixed with Herbal
Performances and Meat Cholesterol Content of Fat Tail Sheep Fed
Diets Supplemented with Sardinella Fish Oil Based
Ca-soap Mixed with Herbal
A. Sudarman* & D.A. Astuti
Department of Nutrition and Feed Technology
Faculty of Animal Science, Bogor Agricultural University
Jl. Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia
*e-mail: a_sudarman@yahoo.com
Abstract
Previous results showed that sardinella fish oil based Ca-soap supplementaion
at level a of 3.0% in ration reduced meat LDL cholesterol of lamb by 49% and
improved feed utilization efficiency by 13%. However, sardinella fish oil based
Ca-soap had bad fishy smell causing low palatability of feed and reduced feed
intake. In Indonesian tradition, fishy smell is ussualy overcomed by applying
herbal. This experiment aimed to elaborate herbal meal addition (ginger, turmeric,
and beluntas leaf) into sardinella fish oil based Ca-soap in reducing its fishy
smell, and subsequently improve its palatability. Sixteen male fat tail sheep were
allocated into four experimental treatments with four replicates and arranged in
a block randomized design. The experimental period lasted for 14 weeks and the
animals had free access to feed (concentrate: native grass in ratio of 1:1) and water.
At the end of experiment, two sheep from each treatment were slaughtered. The
treatments were T0 (without herbal addition), T1 (with turmeric addition), T2 (with
ginger addition), and T3 (with beluntas leaf addition). Herbal Ca-soap, at level
of 3%, were mixed thoroughly with feed ingredients to produce concentrate diet.
The variables measured were feed intake, daily weight gain, feed conversion ratio,
blood triglicerides and meat cholesterol content. The results showed that addition
of sardinella fish oil based Ca-soap mixed with herbal slightly reduced feed intake
and daily gain, but improved feed efficiency utilization. Herbal addition reduced
cholesterol, but increased triglicerides content of blood plasm. Herbal addition,
except for beluntas leaf, also reduced cholesterol content of meat. It is concluded
that sheep fed sardinella oil based Ca-soap with turmeric addition had better
performance and lower cholesterol of meat.
Key words: calcium-soap, herbal, meat cholesterol, sardinella fish oil
Proceeding of the 2nd International Seminar on Animal Industry | Jakarta, 5-6 July 2012
497
Introducton
Rumnant meat contans hgh saturated fatty acds (laurate, myrstate, and palmtate) that caused hgh cholesterol n the blood plasm (Grande, 1975). Substtuton saturated fatty acd wth polyunsaturated fatty acd could reduce total cholesterol, ncludng LDL-cholesterol (Marsc and Yodce, 1992). Lamb contans hgher
cholesterol than beef, .e. 94 mg/100g vs 87 mg/100g. People that consumed food
contanng hgh cholesterol can cause atherosclerosis leadng to coronary heart dsesase. Omega-3
mega-3 polyunsaturated fatty acds (PUFA) can reduce rsk of atheroscleross (IGER, 2003). Source of PUFA contanng hgh omega-3 concentraton s fsh
ol, e.g., from sardnella fsh. Sardnella fsh ol s easy to obtan n Indonesa. It s
a waste product of fsh cannng ndustry spreaded out n eastern part of East Java
Provnce.
Results of the prevous experment showed that feedng sardnella ol based
Ca-soap at level of 3.0% decreased LDL cholesterol of lamb by 49% (Sudarman
et al., 2008a) and feed utlzaton effcency ncreased by 13% (Sudarman et al.,
2008b). Ths ndcates that lpd profle of lamb can be altered by feedng Ca-soap.
However, feedng Ca-soap caused feed ntake to decrease. Ths was probably caused
by bad fshy smell of Ca-soap causng the palatablty of det to decrease.
When cookng fsh materals n Indonesa, t s a tradton to add herbals to
overcome the fshy smell. The herbals that are usually used are gnger (Zingiber
(
officinale Rosc.), turmerc (Curcumae domestica) and beluntas leaf (Pluchea indica
Less).
The objectve of ths experment was to evaluate the effects of herbal addton
nto sardnella ol based Ca-soap on the reducton of ts fshy smell. Ths wll,
subsequently, mprove palatablty of the det and performance of sheep, and reduce
low cholesterol lamb.
Materals and methods
Sxteen male growng fat tal sheep (approx. 8 m.o.) were allocated nto four
expermental dets wth four replcates and arranged n a block randomzed desgn..
They were reared n ndvdual cage for two months. Feed (concentrate : natve
grass n rato of 1:1 dry matter base) were gven ad libitum (110% of prevous day
ntake) at 07.00 and 17.00. The
he anmals had free access to water.. The ngredents of
concentrate were pollard, cassava wate meal, coconut meal, rce bran, palm kernel
meal, soybean meal, molasses, CaCO3, urea, and DCP. Nutrents composton
omposton based
on proxmate analyss was 16% crude proten, 5% of extract ether, 10% crude fber
and totally had70% TDN.
The expermental treatments were T1= basal det (contanng 5% fat), T2= T1
+ 3% Ca-soap wth turmerc meal, T3= T1 + 3% Ca-soap wth gnger meal, and T4=
498
Proceeding of the 2nd International Seminar on Animal Industry | Jakarta, 5-6 July 2012
T1 + 3% Ca-soap wth beluntas leaf meal. Ca-soap complex was made based on
the method used n the prevous experment (Sudarman et al., 2008b) and the herbal
were added before mxng wth other feed ngredents for makng concentrate.
At the end of experment, blood samples from two sheep of each treatment
were collected from jugular ven for analyzng cholesterol and trglcerde contents of
blood plasm. Total cholesterol was measured by the CHOD-PAP method. Trglycerde
determnatons were performed usng the GPO-PAP method.. Subsequently, two
sheep of each treatment were slaughtered for analyzng meat cholesterol content
usng Leberman Burchard method (Klener and Dott, 1962)..
Data of sheep performances were subjected to Analyzes of Varance (ANOVA)
and any dfferent means were further tested usng LSD (Steel and Torre, 1980).
Other data were analyzed usng descrptve statstcs. The varables measured were
dry matter ntake, weght gan, feed converson rato, blood plasm cholesterol and
trglcerdes, and meat cholesterol content.
Results and dscussons
Range of ambent temperature and humudty durng the expermental study
were 22.2-33.8 °C and 83.7-92.3%, respectvely.
Sheep Performances
Data of dry matter ntake, daly weght gan and feed converson rato (FCR)
were shown n Table 1. Dry mattter ntake of sheep of all treatment groups were not
sgnfcantly dfferent. Ths ndcated that herbal addton was not able to mprove
the palatablty of feed added sardnella ol based Ca-soap. Intake of sheep n ths
experment (4.0% BW vs 2.5% BW) was hgher than that of sheep n prevous
results (Sudarman et al., 2008b). Ths dscrepancy was possbly due to the dfference
n breed of sheep used. Prevous experment used thn tal sheep breed wth average
body weght of 16.9 kg, whle the present experment used thck tal sheep breed
Table 1. Performance of sheep fed sardnella fsh ol base Ca-soap wth dfferent herbal
addton ( ± SD)
Treatments
Dry matter ntake
(g/head/day)
Daly gan
(g/head/day)
FCR
T0
T1
T2
T3
1,080 ± 63
1,058 ± 106
1,021 ± 128
1,041 ± 40
148 ± 26
162 ± 21
148 ± 41
131 ± 15
7.44 ± 1.33
6.60 ± 0.95
7.14 ± 1.29
8.02 ± 0.80
Note: T0 (basal det), T1 (T0 + turmerc), T2 (T0 + gnger), and T3 (T0 + beluntas leaf).
Proceeding of the 2nd International Seminar on Animal Industry | Jakarta, 5-6 July 2012
499
wth average body weght of 25.2 kg. Daly weght gan of sheep of all treatment
groups were also not sgnfcantly dfferent, but turmerc addton resulted n 9.5%
daly gan hgher than that of control group. Feed utlzaton effcency (FCR) was
slghtly better for those gven sardnella ol based Ca-soap added wth turmerc,
eventhough t was not statstcally sgnfcantly dfferent.
Plasm Cholesterol and Triglicerides
Cholesterol and trglcerdes of blood plasm were presented n Table 2. Blood
cholesterol of sheep fed Ca-soap wth herbal addton were slghtly lower than that
group. Blood composton
ncludng
blood
cholesterol
was always"2/"0
n dy
ofcontrol
,//%$)/,&23&1/,".%
".%31*(,*2&1*%&2
/'2)&&0
'&% 2"1%*.&,,"
'*2)/*,#"2&%
namc condton. It s affected by type of feed ngested. Blood trglcerdes of sheep
6*3) %*''&1&.3)&1#","%%*3*/. 9
gven Ca-soal wth all herbal addton were consstently lower than that of control
group.
9
9
Table 2. Blood
of sheep fed sardnella
fsh ol
based Ca cholesterol and and trglserdes
9
9
soap wth dfferent herbal addton
(
±
SD)
9
9
9
9
#"2",%*&3
341-&1*$
(*.(&1".%
#&,4.3"2,&"'
Plasm
Cholesterol
Plasm Trglserdes
Treatments
(mg%)
(mg%)
T0
83.86 ± 11.46
16.85 ± 5.35
&"3 $)/,&23&1/, $/.3&.32 /' ",, (1/402 "1& 01&2&.3&% *. *(41& *5*.( "2/"0 6*3)
T1
74.41 ± 11.60
34.83 ± 20.4741-&1*$"%%*3*/.6"2
)&1#","%%*3*/.*.3/%*&3%*%./3$/.2*23&.3,8"''&$3-&"3$)/,&23&1/,$/.3&.3
60.636*3)
± 27.02
14.27
"#,& 3/ 1&%4$& -&"3T2
$)/,&23&1/, #&33&1 3)".
3)& "%%*3*/. /'30.34
/3)&1±)&1#",2
1&5*/42 1&24,32
"
4%"1-".
2"1%*.&,,"
"2/"0
"3 ,&5&, /'
6*3)/43
T3 2)/6&% 3)"3 "%%*.(
80.71 ±
15.29 /*, #"2&%32.02
± 8.68
)&1#","%%*3*/.%&$1&"2&% $)/,&23&1/,/'
Note: T0 (basal det), T1 (T0 + turmerc), T2 (T0 + gnger), and T3 (T0 + beluntas leaf).
)/,&23&1/,/'-&"3-( (
&1#", 1&"3-&.3
Fgure 1. Cholesterol contents of meat of sheep fed expermental dets: T0 (basal det), T1
)/,&23&1/,$/.3&.32 /'-&"3 /'2)&&0'&%&70&1*-&.3",%*&32
#"2",%*&3
(T0 + turmerc), T2 (T0 + gnger), and T3 (T0 + beluntas leaf)
341-&1*$
(*.(&1".%
#&,4.3"2,&"'
500
Proceeding of the 2nd International Seminar on Animal Industry | Jakarta, 5-6 July 2012
&1#","%%*3*/.*2 ./3 "#,& 3/*-01/5& 0","3"#*,*38 /'%*&3 2400,&-&.3&%6*3) "2/"0'*2)
/*, )&&0 '&% 2"1%*.&,," /*, #"2&% "2/"0 6*3) 341-&1*$ "%%*3*/. )"% #&33&1 0&1'/1-".$& ".%
Meat Cholesterol
Meat cholesterol contents of all groups are presented n Fgure 1. Gvng
Ca-soap wth herbal addton nto det dd not consstently affect meat cholesterol
content. Turmerc addton was able to reduce meat cholesterol better than wth the
addton of other herbals. Prevous
revous results (Sudarman et al., 2008a) showed that
addng sardnella ol based Ca-soap at level of 3% wthout herbal addton decreased
LDL cholesterol of 49%.
Concluson
Herbal addton s not able to mprove palatablty of det supplemented wth
Ca-soap fsh ol. Sheep fed sardnella ol based Ca-soap wth turmerc addton had
better performance and lower cholesterol of meat.
Acknowledgement
We would lke to thanks to the Drectorate General of Hgher Educaton that
provded the fnancal grant for conductng ths research.
References
Grande, F. 1975. Proc. 9th Int. Congr. Nutr. Mexco. 1:346.
IGER (Insttute of Grassland and Envronmental Research). 2003. Healthy Beef.
http://www.seedsohealth.co.uk/articles/healthy_beef.shtml.
Klener, I.S. and L.B. Dott. 1962. Laboratory Instructon n Bochemstry. 6th ed.
The C.V. Mosby Company, New York.
Marsc, V and R. Yodce. 1992. The Detary Role of Monounsaturates. INFORM,
3:681.
Steel, R.G.D. & J.H. Torre. 1980. Prncples and Procedures of Statstcs: A Bometrc Approach. McGraw-Hll Book Co. New York.
Sudarman, A., H. Nuraen and M. Muttakn. 2008a. Penambahan Sabun-kalsum
dar Mnyak Ikan Sardnella dalam Ransum: 2. Pengaruhnya terhadap Sfat
Kma dan Fsk Dagng Domba. Jurnal Ilmu Ternak dan Veterner. 13: 133139.
Sudarman, A., K. G. Wryawan andd H. Markhamah. 2008b. Penambahan Sabun-kalsum dar Mnyak Ikan Sardnella dalam Ransum:: 1. Pengaruhnya terhadap
Tamplan Produks Domba. Meda Peternakan. 31:166-171.
Proceeding of the 2nd International Seminar on Animal Industry | Jakarta, 5-6 July 2012
501
Diets Supplemented with Sardinella Fish Oil Based
Ca-soap Mixed with Herbal
A. Sudarman* & D.A. Astuti
Department of Nutrition and Feed Technology
Faculty of Animal Science, Bogor Agricultural University
Jl. Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia
*e-mail: a_sudarman@yahoo.com
Abstract
Previous results showed that sardinella fish oil based Ca-soap supplementaion
at level a of 3.0% in ration reduced meat LDL cholesterol of lamb by 49% and
improved feed utilization efficiency by 13%. However, sardinella fish oil based
Ca-soap had bad fishy smell causing low palatability of feed and reduced feed
intake. In Indonesian tradition, fishy smell is ussualy overcomed by applying
herbal. This experiment aimed to elaborate herbal meal addition (ginger, turmeric,
and beluntas leaf) into sardinella fish oil based Ca-soap in reducing its fishy
smell, and subsequently improve its palatability. Sixteen male fat tail sheep were
allocated into four experimental treatments with four replicates and arranged in
a block randomized design. The experimental period lasted for 14 weeks and the
animals had free access to feed (concentrate: native grass in ratio of 1:1) and water.
At the end of experiment, two sheep from each treatment were slaughtered. The
treatments were T0 (without herbal addition), T1 (with turmeric addition), T2 (with
ginger addition), and T3 (with beluntas leaf addition). Herbal Ca-soap, at level
of 3%, were mixed thoroughly with feed ingredients to produce concentrate diet.
The variables measured were feed intake, daily weight gain, feed conversion ratio,
blood triglicerides and meat cholesterol content. The results showed that addition
of sardinella fish oil based Ca-soap mixed with herbal slightly reduced feed intake
and daily gain, but improved feed efficiency utilization. Herbal addition reduced
cholesterol, but increased triglicerides content of blood plasm. Herbal addition,
except for beluntas leaf, also reduced cholesterol content of meat. It is concluded
that sheep fed sardinella oil based Ca-soap with turmeric addition had better
performance and lower cholesterol of meat.
Key words: calcium-soap, herbal, meat cholesterol, sardinella fish oil
Proceeding of the 2nd International Seminar on Animal Industry | Jakarta, 5-6 July 2012
497
Introducton
Rumnant meat contans hgh saturated fatty acds (laurate, myrstate, and palmtate) that caused hgh cholesterol n the blood plasm (Grande, 1975). Substtuton saturated fatty acd wth polyunsaturated fatty acd could reduce total cholesterol, ncludng LDL-cholesterol (Marsc and Yodce, 1992). Lamb contans hgher
cholesterol than beef, .e. 94 mg/100g vs 87 mg/100g. People that consumed food
contanng hgh cholesterol can cause atherosclerosis leadng to coronary heart dsesase. Omega-3
mega-3 polyunsaturated fatty acds (PUFA) can reduce rsk of atheroscleross (IGER, 2003). Source of PUFA contanng hgh omega-3 concentraton s fsh
ol, e.g., from sardnella fsh. Sardnella fsh ol s easy to obtan n Indonesa. It s
a waste product of fsh cannng ndustry spreaded out n eastern part of East Java
Provnce.
Results of the prevous experment showed that feedng sardnella ol based
Ca-soap at level of 3.0% decreased LDL cholesterol of lamb by 49% (Sudarman
et al., 2008a) and feed utlzaton effcency ncreased by 13% (Sudarman et al.,
2008b). Ths ndcates that lpd profle of lamb can be altered by feedng Ca-soap.
However, feedng Ca-soap caused feed ntake to decrease. Ths was probably caused
by bad fshy smell of Ca-soap causng the palatablty of det to decrease.
When cookng fsh materals n Indonesa, t s a tradton to add herbals to
overcome the fshy smell. The herbals that are usually used are gnger (Zingiber
(
officinale Rosc.), turmerc (Curcumae domestica) and beluntas leaf (Pluchea indica
Less).
The objectve of ths experment was to evaluate the effects of herbal addton
nto sardnella ol based Ca-soap on the reducton of ts fshy smell. Ths wll,
subsequently, mprove palatablty of the det and performance of sheep, and reduce
low cholesterol lamb.
Materals and methods
Sxteen male growng fat tal sheep (approx. 8 m.o.) were allocated nto four
expermental dets wth four replcates and arranged n a block randomzed desgn..
They were reared n ndvdual cage for two months. Feed (concentrate : natve
grass n rato of 1:1 dry matter base) were gven ad libitum (110% of prevous day
ntake) at 07.00 and 17.00. The
he anmals had free access to water.. The ngredents of
concentrate were pollard, cassava wate meal, coconut meal, rce bran, palm kernel
meal, soybean meal, molasses, CaCO3, urea, and DCP. Nutrents composton
omposton based
on proxmate analyss was 16% crude proten, 5% of extract ether, 10% crude fber
and totally had70% TDN.
The expermental treatments were T1= basal det (contanng 5% fat), T2= T1
+ 3% Ca-soap wth turmerc meal, T3= T1 + 3% Ca-soap wth gnger meal, and T4=
498
Proceeding of the 2nd International Seminar on Animal Industry | Jakarta, 5-6 July 2012
T1 + 3% Ca-soap wth beluntas leaf meal. Ca-soap complex was made based on
the method used n the prevous experment (Sudarman et al., 2008b) and the herbal
were added before mxng wth other feed ngredents for makng concentrate.
At the end of experment, blood samples from two sheep of each treatment
were collected from jugular ven for analyzng cholesterol and trglcerde contents of
blood plasm. Total cholesterol was measured by the CHOD-PAP method. Trglycerde
determnatons were performed usng the GPO-PAP method.. Subsequently, two
sheep of each treatment were slaughtered for analyzng meat cholesterol content
usng Leberman Burchard method (Klener and Dott, 1962)..
Data of sheep performances were subjected to Analyzes of Varance (ANOVA)
and any dfferent means were further tested usng LSD (Steel and Torre, 1980).
Other data were analyzed usng descrptve statstcs. The varables measured were
dry matter ntake, weght gan, feed converson rato, blood plasm cholesterol and
trglcerdes, and meat cholesterol content.
Results and dscussons
Range of ambent temperature and humudty durng the expermental study
were 22.2-33.8 °C and 83.7-92.3%, respectvely.
Sheep Performances
Data of dry matter ntake, daly weght gan and feed converson rato (FCR)
were shown n Table 1. Dry mattter ntake of sheep of all treatment groups were not
sgnfcantly dfferent. Ths ndcated that herbal addton was not able to mprove
the palatablty of feed added sardnella ol based Ca-soap. Intake of sheep n ths
experment (4.0% BW vs 2.5% BW) was hgher than that of sheep n prevous
results (Sudarman et al., 2008b). Ths dscrepancy was possbly due to the dfference
n breed of sheep used. Prevous experment used thn tal sheep breed wth average
body weght of 16.9 kg, whle the present experment used thck tal sheep breed
Table 1. Performance of sheep fed sardnella fsh ol base Ca-soap wth dfferent herbal
addton ( ± SD)
Treatments
Dry matter ntake
(g/head/day)
Daly gan
(g/head/day)
FCR
T0
T1
T2
T3
1,080 ± 63
1,058 ± 106
1,021 ± 128
1,041 ± 40
148 ± 26
162 ± 21
148 ± 41
131 ± 15
7.44 ± 1.33
6.60 ± 0.95
7.14 ± 1.29
8.02 ± 0.80
Note: T0 (basal det), T1 (T0 + turmerc), T2 (T0 + gnger), and T3 (T0 + beluntas leaf).
Proceeding of the 2nd International Seminar on Animal Industry | Jakarta, 5-6 July 2012
499
wth average body weght of 25.2 kg. Daly weght gan of sheep of all treatment
groups were also not sgnfcantly dfferent, but turmerc addton resulted n 9.5%
daly gan hgher than that of control group. Feed utlzaton effcency (FCR) was
slghtly better for those gven sardnella ol based Ca-soap added wth turmerc,
eventhough t was not statstcally sgnfcantly dfferent.
Plasm Cholesterol and Triglicerides
Cholesterol and trglcerdes of blood plasm were presented n Table 2. Blood
cholesterol of sheep fed Ca-soap wth herbal addton were slghtly lower than that
group. Blood composton
ncludng
blood
cholesterol
was always"2/"0
n dy
ofcontrol
,//%$)/,&23&1/,".%
".%31*(,*2&1*%&2
/'2)&&0
'&% 2"1%*.&,,"
'*2)/*,#"2&%
namc condton. It s affected by type of feed ngested. Blood trglcerdes of sheep
6*3) %*''&1&.3)&1#","%%*3*/. 9
gven Ca-soal wth all herbal addton were consstently lower than that of control
group.
9
9
Table 2. Blood
of sheep fed sardnella
fsh ol
based Ca cholesterol and and trglserdes
9
9
soap wth dfferent herbal addton
(
±
SD)
9
9
9
9
#"2",%*&3
341-&1*$
(*.(&1".%
#&,4.3"2,&"'
Plasm
Cholesterol
Plasm Trglserdes
Treatments
(mg%)
(mg%)
T0
83.86 ± 11.46
16.85 ± 5.35
&"3 $)/,&23&1/, $/.3&.32 /' ",, (1/402 "1& 01&2&.3&% *. *(41& *5*.( "2/"0 6*3)
T1
74.41 ± 11.60
34.83 ± 20.4741-&1*$"%%*3*/.6"2
)&1#","%%*3*/.*.3/%*&3%*%./3$/.2*23&.3,8"''&$3-&"3$)/,&23&1/,$/.3&.3
60.636*3)
± 27.02
14.27
"#,& 3/ 1&%4$& -&"3T2
$)/,&23&1/, #&33&1 3)".
3)& "%%*3*/. /'30.34
/3)&1±)&1#",2
1&5*/42 1&24,32
"
4%"1-".
2"1%*.&,,"
"2/"0
"3 ,&5&, /'
6*3)/43
T3 2)/6&% 3)"3 "%%*.(
80.71 ±
15.29 /*, #"2&%32.02
± 8.68
)&1#","%%*3*/.%&$1&"2&% $)/,&23&1/,/'
Note: T0 (basal det), T1 (T0 + turmerc), T2 (T0 + gnger), and T3 (T0 + beluntas leaf).
)/,&23&1/,/'-&"3-( (
&1#", 1&"3-&.3
Fgure 1. Cholesterol contents of meat of sheep fed expermental dets: T0 (basal det), T1
)/,&23&1/,$/.3&.32 /'-&"3 /'2)&&0'&%&70&1*-&.3",%*&32
#"2",%*&3
(T0 + turmerc), T2 (T0 + gnger), and T3 (T0 + beluntas leaf)
341-&1*$
(*.(&1".%
#&,4.3"2,&"'
500
Proceeding of the 2nd International Seminar on Animal Industry | Jakarta, 5-6 July 2012
&1#","%%*3*/.*2 ./3 "#,& 3/*-01/5& 0","3"#*,*38 /'%*&3 2400,&-&.3&%6*3) "2/"0'*2)
/*, )&&0 '&% 2"1%*.&,," /*, #"2&% "2/"0 6*3) 341-&1*$ "%%*3*/. )"% #&33&1 0&1'/1-".$& ".%
Meat Cholesterol
Meat cholesterol contents of all groups are presented n Fgure 1. Gvng
Ca-soap wth herbal addton nto det dd not consstently affect meat cholesterol
content. Turmerc addton was able to reduce meat cholesterol better than wth the
addton of other herbals. Prevous
revous results (Sudarman et al., 2008a) showed that
addng sardnella ol based Ca-soap at level of 3% wthout herbal addton decreased
LDL cholesterol of 49%.
Concluson
Herbal addton s not able to mprove palatablty of det supplemented wth
Ca-soap fsh ol. Sheep fed sardnella ol based Ca-soap wth turmerc addton had
better performance and lower cholesterol of meat.
Acknowledgement
We would lke to thanks to the Drectorate General of Hgher Educaton that
provded the fnancal grant for conductng ths research.
References
Grande, F. 1975. Proc. 9th Int. Congr. Nutr. Mexco. 1:346.
IGER (Insttute of Grassland and Envronmental Research). 2003. Healthy Beef.
http://www.seedsohealth.co.uk/articles/healthy_beef.shtml.
Klener, I.S. and L.B. Dott. 1962. Laboratory Instructon n Bochemstry. 6th ed.
The C.V. Mosby Company, New York.
Marsc, V and R. Yodce. 1992. The Detary Role of Monounsaturates. INFORM,
3:681.
Steel, R.G.D. & J.H. Torre. 1980. Prncples and Procedures of Statstcs: A Bometrc Approach. McGraw-Hll Book Co. New York.
Sudarman, A., H. Nuraen and M. Muttakn. 2008a. Penambahan Sabun-kalsum
dar Mnyak Ikan Sardnella dalam Ransum: 2. Pengaruhnya terhadap Sfat
Kma dan Fsk Dagng Domba. Jurnal Ilmu Ternak dan Veterner. 13: 133139.
Sudarman, A., K. G. Wryawan andd H. Markhamah. 2008b. Penambahan Sabun-kalsum dar Mnyak Ikan Sardnella dalam Ransum:: 1. Pengaruhnya terhadap
Tamplan Produks Domba. Meda Peternakan. 31:166-171.
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