Studi In vitro dan In vivo Efek Radioprotektif Rosela (Hibiscus Sabdariffa Linn) terhadap Radiasi Ionisasi Radiodiagnostik Berulang
STUDI IN-VITRO DAN IN-VIVO EFEK RADIOPROTEKTIF
ROSELA (Hibiscus sabdariffa Linn.) TERHADAP RADIASI
IONISASI RADIODIAGNOSTIK BERULANG
MOKHAMAD FAKHRUL ULUM
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
2012
i
STUDI IN-VITRO DAN IN-VIVO EFEK RADIOPROTEKTIF
ROSELA (Hibiscus sabdariffa Linn.) TERHADAP RADIASI
IONISASI RADIODIAGNOSTIK BERULANG
OLEH
MOKHAMAD FAKHRUL ULUM
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
2012
i
ii
PERNYATAAN MENGENAI TESIS DAN
SUMBER INFORMASI
Dengan ini saya menyatakan bahwa Tesis “Studi In-vitro dan In-vivo Efek
Radioprotektif Rosela (Hibiscus Sabdariffa Linn.) terhadap Radiasi Ionisasi
Radiodiagnostik Berulang” adalah karya saya sendiri dan belum diajukan dalam
bentuk apapun kepada perguruan tinggi manapun. Sumber informasi yang berasal
atau dikutip dari karya yang diterbitkan maupun tidak diterbitkan dari penulis lain
telah disebutkan dalam teks dan dicantumkan dalam Daftar Pustaka di bagian
akhir Tesis ini.
Bogor, Februari 2012
Mokhamad Fakhrul Ulum
NRP: B351090021
iii
iv
ABSTRACT
The purpose of this study is to determine the natural radioprotective effect of
ethanolic extract of roselle (Hibiscus sabdariffa L.) through the in-vitro and invivo study of mice that were exposed by ionizing radiation from recurrent
radiodiagnostic. In in-vitro study, splenic lymphocytes were cultured in different
concentration of ethanolic extract of roselle. Splenic lymphocytes were divided in
two groups; with radiation exposure and without radiation exposure. The
numbers of lymphocyte were calculated at 1, 2 and 3 days after radiation of each
group. In in-vivo study, mice supplemented with ethanolic extract of roselle dose
50 mg/kg body weight prior to radiation for 60 days and 30 days after recovery
for clinical and pathological evaluation. Radiation from radiodiagnostic
radiography exposure performs repeated every 2 days. Forty eight adult mice
were divided in 4 groups; control (K), radiation (P), roselle (R) and roselle with
radiation (RP). Clinical data were collected for live survival, body weight, and
peripheral blood characteristic. Pathological data were collected for relative
organ weight and histopathological finding of bone marrow and spleen at 4 and 8
weeks after radiation; 8 and 12 weeks after 30 days recovery. The in-vitro study
clearly showed that roselle enhances the splenic lymphocyte proliferation and
maintained amount of cells (p
ROSELA (Hibiscus sabdariffa Linn.) TERHADAP RADIASI
IONISASI RADIODIAGNOSTIK BERULANG
MOKHAMAD FAKHRUL ULUM
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
2012
i
STUDI IN-VITRO DAN IN-VIVO EFEK RADIOPROTEKTIF
ROSELA (Hibiscus sabdariffa Linn.) TERHADAP RADIASI
IONISASI RADIODIAGNOSTIK BERULANG
OLEH
MOKHAMAD FAKHRUL ULUM
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
2012
i
ii
PERNYATAAN MENGENAI TESIS DAN
SUMBER INFORMASI
Dengan ini saya menyatakan bahwa Tesis “Studi In-vitro dan In-vivo Efek
Radioprotektif Rosela (Hibiscus Sabdariffa Linn.) terhadap Radiasi Ionisasi
Radiodiagnostik Berulang” adalah karya saya sendiri dan belum diajukan dalam
bentuk apapun kepada perguruan tinggi manapun. Sumber informasi yang berasal
atau dikutip dari karya yang diterbitkan maupun tidak diterbitkan dari penulis lain
telah disebutkan dalam teks dan dicantumkan dalam Daftar Pustaka di bagian
akhir Tesis ini.
Bogor, Februari 2012
Mokhamad Fakhrul Ulum
NRP: B351090021
iii
iv
ABSTRACT
The purpose of this study is to determine the natural radioprotective effect of
ethanolic extract of roselle (Hibiscus sabdariffa L.) through the in-vitro and invivo study of mice that were exposed by ionizing radiation from recurrent
radiodiagnostic. In in-vitro study, splenic lymphocytes were cultured in different
concentration of ethanolic extract of roselle. Splenic lymphocytes were divided in
two groups; with radiation exposure and without radiation exposure. The
numbers of lymphocyte were calculated at 1, 2 and 3 days after radiation of each
group. In in-vivo study, mice supplemented with ethanolic extract of roselle dose
50 mg/kg body weight prior to radiation for 60 days and 30 days after recovery
for clinical and pathological evaluation. Radiation from radiodiagnostic
radiography exposure performs repeated every 2 days. Forty eight adult mice
were divided in 4 groups; control (K), radiation (P), roselle (R) and roselle with
radiation (RP). Clinical data were collected for live survival, body weight, and
peripheral blood characteristic. Pathological data were collected for relative
organ weight and histopathological finding of bone marrow and spleen at 4 and 8
weeks after radiation; 8 and 12 weeks after 30 days recovery. The in-vitro study
clearly showed that roselle enhances the splenic lymphocyte proliferation and
maintained amount of cells (p