Uji Aktivitas Penghambatan Diare Ekstrak Etanol Daun Srikaya (Annona squamosa L.) terhadap Mencit Jantan
Lampiran 1. Surat hasil identifikasi tumbuhan
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Lampiran 2. Surat rekomendasi persetujuan etik penelitian
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Lampiran 3. Gambar tanaman srikaya (Annona squamosa L.)
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Lampiran 4. Gambar daun segar dan serbuk simplisia daun srikaya
Gambar daun srikaya segar
Serbuk simplisia daun srikaya
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Lampiran 5. Bagan kerja penelitian
1.
Pemeriksaan karakteristik dan skrining fitokimia serta pembuatan Ekstrak
Etanol Daun Srikaya (EEDS)
Daun srikaya
Dikumpulkan, dicuci,
ditiriskan dan ditimbang berat
basahnya
Dikeringkan di lemari
pengering
Simplisia daun srikaya
Ditimbang berat kering
simplisia
Dihaluskan
Serbuk simplisia
Diperkolasi
menggunakan
etanol 96%
Karakterisasi
meliputi
- Makroskopik
- Mikroskopik
- Kadar air
- Kadar sari larut
dalam air
- Kadar sari larut
dalam etanol
- Kadar abu total
- Kadar abu tidak
larut asam
Skrining fitokimia
meliputi:
- Flavonoid
- Alkaloid
- Saponin
- Tanin
- Glikosida
- Steroid/
terpenoid
Perkolat
Di-rotary evaporator
Ekstrak etanol
daun srikaya
(EEDS)
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Lampiran 5. (Lanjutan)
2. Pengujian aktivitas antidiare EEDS pada mencit jantan yang diinduksi oleh
minyak jarak
30 ekor mencit jantan
Dibagi secara acak menjadi 6 kelompok
Ditimbang, dicatat berat badannya dan diberi
penandaan pada ekornya
Diberi perlakuan setiap kelompok : suspensi NaCMC 0,5% dosis 1% BB; suspensi loperamid dosis
0,52 mg/kg BB; EEDS dosis 50, 100, 200, dan 400
mg/kg BB secara oral
Diberi minyak jarak 0,5 ml tiap mencit secara oral
setelah 1 jam pemberian perlakuan
Diamati mencit selama 5 jam selang waktu 30
menit
Onset diare
(menit)
Frekuensi diare
(kali)
Konsistensi dan
berat feses
(mg)
Durasi diare
(menit)
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Lampiran 5. (Lanjutan)
3. Pengujian aktivitas antimotilitas EEDS dengan metode transit intestinal
30 ekor mencit jantan
Dibagi secara acak menjadi 6 kelompok
Ditimbang, dicatat berat badannya dan diberi
penandaan pada ekornya
Diberi perlakuan setiap kelompok : suspensi NaCMC 0,5% dosis 1% BB; suspensi loperamid
dosis 0,52 mg/kg BB; EEDS dosis 50, 100, 200,
dan 400 mg/kg BB secara oral
Diberi minyak jarak 0,5 ml tiap mencit secara oral
setelah 1 jam pemberian perlakuan
Diberi tinta cina sebanyak 0,1 ml/10 g secara oral
setelah 1 jam pemberian minyak jarak
Dibiarkan 1 jam, kemudian semua hewan
dikorbankan dengan cara dislokasi leher
Dilakukan pembedahan dan ususnya dikeluarkan
Diukur panjang usus yang dilalui marker tinta
cina
Persen lintasan marker (%)
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Lampiran 6. Gambar pemeriksaan mikroskopik serbuk simplisia daun srikaya
perbesaran 10 X 40
1
2
1
3 2
3
Keterangan:
1. Stomata tipe parasitik
2. Trikoma multiseluler
3. Berkas pengangkut tipe spiral
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Lampiran 7. Perhitungan hasil pemeriksaan karakteristik simplisia daun srikaya
1. Perhitungan penetapan kadar air
Kadar air simplisia =
No.
1
2
3
Berat Sampel (g)
5,0578
5,0412
5,0502
x 100%
Volume awal (ml)
1,9
1,9
1,9
a. Kadar air 1
=
x 100% = 9,88%
b. Kadar air 2
=
x 100% = 9,91%
c. Kadar air 3
=
x 100% = 9,90%
Volume akhir (ml)
2,4
2,4
2,4
Rata-rata kadar air =
= 9,89%
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Lampiran 7. (Lanjutan)
2. Perhitungan penetapan kadar sari larut air
Kadar sari larut air =
No.
1.
2.
3.
a.
100%
Berat sampel (g)
5,0067
5,0046
5,0041
Kadar sari larut air 1
Berat sari (g)
0,3258
0,3043
0,2930
=
100%
=
100%
=
100%
= 32,53%
b.
Kadar sari larut air 2
= 30,40%
c.
Kadar sari larut air 3
= 29,27%
Rata-rata kadar sari larut air
= 30,73%
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Lampiran 7. (Lanjutan)
3.
Perhitungan penetapan kadar sari larut etanol
Kadar sari larut etanol =
No.
1.
2.
3.
100%
Berat sampel (g)
5,0023
5,0024
5,0024
Berat sari (g)
0,1640
0,1741
0,1712
a. Kadar sari larut etanol 1 =
100%
= 16,39%
100%
b. Kadar sari larut etanol 2 =
= 17,40%
c. Kadar sari larut etanol 3 =
100%
= 17,11%
Rata-rata kadar sari larut etanol =
= 16,96%
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Lampiran 7. (Lanjutan)
4.
Perhitungan penetapan kadar abu total
Kadar abu total
No.
1
2
3
=
100%
Berat Sampel (g)
2,0249
2,0098
2,0068
a. Kadar abu total 1 =
Berat abu (g)
0,0682
0,0653
0,0707
100%
= 3,36%
100%
b. Kadar abu total 2 =
= 3,24%
c. Kadar abu total 3 =
100%
= 3,52%
Rata- rata kadar abu total
=
= 3,37%
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Lampiran 7. (Lanjutan)
5. Perhitungan kadar abu tidak larut dalam asam
Kadar abu tidak larut dalam asam
No.
1
2
3
=
Berat Sampel (g)
2,0249
2,0098
2,0068
a. Kadar abu tidak larut asam 1
100%
Berat abu (g)
0,0095
0,0070
0,0187
100%
=
= 0,46%
b. Kadar abu tidak larut asam 2
=
100%
= 0,34%
c. Kadar air tidak larut asam 3
=
100%
= 0,93%
Rata- rata kadar air tidak larut asam =
= 0,57%
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Lampiran 8. Gambar alat dan bahan yang diperlukan
Spuit dan oral sonde
Minyak jarak
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Lampiran 8. (Lanjutan)
Peralatan bedah hewan
Tinta cina
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Lampiran 9. Gambar pengamatan hewan
Pengamatan hewan setelah diinduksi minyak jarak
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Lampiran 10. Gambar hewan sebelum dan sesudah dibedah
Posisi hewan sebelum dibedah
Posisi hewan setelah dibedah
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Lampiran 11. Gambar usus halus yang dilintasi marker tinta cina
55 cm
50 cm
Lintasan marker pada kelompok kontrol negatif
13,5 cm
41 cm
Lintasan marker pada kelompok kontrol positif
48 cm
46 cm
42 cm
Lintasan marker pada kelompok EEDS dosis 50 mg/kg BB
30 cm
Lintasan marker pada kelompok EEDS dosis 100 mg/kg BB
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Lampiran 11. (Lanjutan)
59 cm
30 cm
Lintasan marker pada kelompok EEDS dosis 200 mg/kg BB
46,5 cm
18,5 cm
Lintasan marker pada kelompok EEDS dosis 400 mg/kg BB
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Lampiran 12. Contoh perhitungan dosis
Tabel konversi dosis antara jenis hewan dengan manusia (Darmono, 2011).
Tabel volume maksimal larutan sediaan uji yang diberikan pada hewan
Volume maksimal (ml)
Jenis hewan uji
i.v
i.m
i.p
s.c
Mencit
0,5
0,05
1,0
0,5-1,0
(20-30)
p.o
1,0
Tikus (100 g)
1,0
0,1
2,5
2,5
5,0
Hamster (50 g)
-
0,1
1-2
2,5
2,5
Marmut (250 g)
-
0,25
2-5
5,0
10,0
Merpati (300 g)
2,0
0,5
2,0
2,0
10,0
Kelinci (2,5 kg)
5-10
0,5
10-20
5-10
20,0
Kucing
(3 kg)
5-10
1,0
10-20
5-10
50,0
Anjing
(5 kg)
10-20
5,0
20-50
10,0
100,0
(Harmita dan Radji, 2008)
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Lampiran 12. (Lanjutan)
1. Perhitungan dosis suspensi Na-CMC 0,5% dosis 1% BB
- Na-CMC 0,5% =
= 5 mg/ml
- Dosis 1% BB diberikan pada mencit 25 g
-
× 25 g = 0,25 ml
- Volume yang diberikan pada mencit = 0,25 ml
2. Perhitungan dosis suspensi loperamid HCl
®
- Tiap tablet Lodia mengandung loperamid HCl 2 mg
- Dosis loperamid HCl untuk manusia adalah 2 tablet = 4 mg loperamid HCl
dan dosis maksimal 16 mg/hari.
- Dosis untuk mencit 20 g adalah:
( 4 mg – 16 mg) x 0,0026 = 0,0104 mg – 0,0416 mg
Dosis yang digunakan adalah 0,0104 mg/20 g = 0,52 mg/kg BB
- Menurut FI Edisi III, penetapan kadar tablet memerlukan 20 tablet, maka
®
diambil 20 tablet Lodia kemudian digerus dan ditimbang berat totalnya =
3160 mg
®
- Berat bahan aktif loperamid HCl dalam 20 tablet Lodia
adalah 2 mg/tab x
20 tab = 40 mg.
®
- Maka serbuk Lodia yang digunakan adalah
x 3160 mg = 41,08 mg ≈ 41 mg
- Cara pembuatan suspensi loperamid HCl
®
Ditimbang serbuk Lodia
sebanyak 41 mg digerus kemudian dilarutkan
dengan Na-CMC 0,5% hingga 10 ml (konsentrasi = 0,0052%)
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Lampiran 12. (Lanjutan)
- Volume suspensi loperamid yang diberikan (misal berat badan = 25 g)
× 25 g ×
= 0,25 ml
3. Perhitungan dosis suspensi EEDS
- Suspensi EEDS dibuat dengan 4 variasi konsentrasi yaitu 0,5%, 1%, 2%,
dan 4%. EEDS ditimbang 50 mg, 100 mg, 200 mg dan 400 mg, masingmasing digerus didalam lumpang, ditambahkan suspensi Na-CMC 0,5%
sedikit demi sedikit dan dicukupkan sampai 10 ml.
- Volume suspensi EEDS 2% yang diberikan (misal berat badan = 23 g)
dengan dosis 200 mg/kg BB adalah
-
× 23 g ×
= 0,23 ml
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Lampiran 13. Data onset diare
Hewan kePerlakuan
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 50 mg/kg BB
EEDS dosis 100 mg/kg BB
EEDS dosis 200 mg/kg BB
EEDS dosis 400 mg/kg BB
1
2
3
4
5
80
88
94
75
83
RataRata
(menit)
84
124
138
147
131
156
139,2
90
109
113
136
82
103
127
142
97
98
110
129
105
107
118
125
94
114
124
117
93,6
106,2
118,4
129,5
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Lampiran 14. Data frekuensi diare
Perlakuan
Suspensi
Na-CMC
0,5%
Suspensi
loperamid
0,52 mg/kg
BB
EEDS
dosis 50
mg/kg BB
EEDS
dosis 100
mg/kg BB
EEDS
dosis 200
mg/kg BB
EEDS
dosis 400
mg/kg BB
1
0
0
0
0
0
2
0
0
0
0
0
3
1
1
2
1
1
Pengamatan ke4
5
6
7
3
2
1
2
1
1
2
2
2
1
1
1
1
3
2
3
2
2
1
2
8
1
1
1
2
1
9
1
1
1
2
1
10
1
1
1
1
1
0
0
0
0
1
2
2
0
0
0
Jumlah
(kali)
12
10
10
15
11
58
5
2
0
0
0
0
2
2
0
2
0
0
6
3
4
5
Jumlah
1
2
3
4
5
Jumlah
1
2
3
4
5
Jumlah
0
0
0
0
0
0
0
0
0
0
0
0
1
1
0
0
1
1
0
2
1
3
0
0
1
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
0
0
0
1
3
1
1
2
2
2
3
3
2
3
0
3
2
1
0
1
0
0
2
2
1
1
2
1
2
1
0
2
2
0
0
1
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
2
2
1
1
2
0
1
3
2
2
2
3
0
2
1
2
1
2
1
2
2
0
0
1
2
1
2
1
1
0
0
0
0
0
5
4
3
23
11
9
9
10
11
50
10
9
9
7
8
43
1
2
3
4
5
Jumlah
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
1
2
0
2
2
2
2
2
1
1
2
0
1
1
1
1
2
3
0
0
1
0
1
1
2
0
2
2
0
0
0
0
0
0
0
0
0
1
1
2
2
0
0
6
7
7
8
9
37
6
2
0
0
0
0
1
2
2
2
2
0
8
3
4
5
Jumlah
0
0
0
0
0
0
0
0
0
0
0
1
1
2
0
2
2
3
1
0
2
0
2
2
1
1
0
0
0
0
5
7
8
34
Mencit
ke1
2
3
4
5
Jumlah
1
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Lampiran 15. Data konsistensi dan berat feses
Perlakuan
Suspensi Na-CMC
0,5%
Suspensi loperamid
dosis 0,52 mg/kg BB
EEDS dosis 50 mg/kg
BB
EEDS dosis 100
mg/kg BB
EEDS dosis 200
mg/kg BB
EEDS dosis 400
mg/kg BB
Hewan ke
Padat
0,41
0,39
0,52
0,24
0,55
2,11
0,16
0,09
0,10
0,23
0,18
0,76
0,35
0,31
0,53
0,27
0,42
1,88
0,31
0,28
0,26
0,47
0,12
1,45
0,34
0,14
0,28
0,45
0,10
1,31
0,17
0,11
0,20
0,25
0,31
1,04
1
2
3
4
5
Jumlah
1
2
3
4
5
Jumlah
1
2
3
4
5
Jumlah
1
2
3
4
5
Jumlah
1
2
3
4
5
Jumlah
1
2
3
4
5
Jumlah
Berat feses (gram)
Berair
Lembek
0,23
1,29
0,62
1,64
0,43
1,37
0,51
1,55
0,50
1,32
2,29
7,17
0,11
0,68
0,13
0,46
0,23
0,75
0,21
0,47
0,19
0,54
0,87
2,90
0,46
1,05
0,31
0,90
0,42
1,51
0,25
1,46
0,37
1,39
1,81
6,31
0,28
0,58
0,25
0,77
0,14
0,80
0,29
1,13
0,36
1,26
1,32
4,54
0,13
0,98
0,32
0,75
0,17
0,99
0,22
0,53
0,29
0,57
1,13
3,82
0,39
0,88
0,17
0,65
0,24
0,89
0,21
0,43
0,10
0,47
1,11
3,32
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Lampiran 16. Data durasi diare
Hewan kePerlakuan
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 50 mg/kg BB
EEDS dosis 100 mg/kg BB
EEDS dosis 200 mg/kg BB
EEDS dosis 400 mg/kg BB
1
2
3
4
5
193
199
187
219
192
RataRata
(menit)
198
78
83
100
82
94
87,4
164
144
128
91
169
145
141
103
181
152
127
119
155
155
126
128
179
151
135
112
169,6
149,4
131,4
110,6
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Lampiran 17. Data persentase lintas marker
Perlakuan
Suspensi Na-CMC 0,5%
Suspensi Loperamid
dosis 0,52 mg/kg BB
EEDS dosis 50 mg/kg
BB
EEDS dosis 100 mg/kg
BB
EEDS dosis 200 mg/kg
BB
EEDS dosis 400 mg/kg
BB
Hewan
ke
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
Panjang
usus
dilintasi
marker
(cm)
43
37,5
50
43
56
20
22,5
35
13,5
21
42
43
41
50
47,5
30
33
37,5
28,5
34
30
22
30
30
25
30
18,5
34
37,5
28
Panjang
usus
% Lintasan
seluruhnya
(%)
(cm)
46
42,5
55
54
60
41,5
42,5
61
41
58
48
47
46
58
62
46
48
52
46
53
44
48
47
59
47
50,5
46,5
60
60
46
93,47
88,23
90,90
79,62
93,33
48,19
52,94
57,37
32,92
36,20
87,50
91,48
89,13
86,20
76,61
65,21
68,75
72,11
61,95
64,15
68,18
45,83
63,82
50,84
53,19
59,40
39,78
56,67
62,50
60,86
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Lampiran 18. Analisis statistik onset diare
Tests of Normality
a
Kolmogorov-Smirnov
Kelompok
Statistic
Waktu Awal Suspensi Na-CMC 0,5%
Diare
Suspensi Loperamid
0,52 mg/kg BB
df
.154
Shapiro-Wilk
Sig.
5
Statistic
df
Sig.
.200
*
.990
5
.981
.982
5
.947
.141
5
.200
*
EEDS dosis 50 mg/kg
BB
.145
5
.200
*
.997
5
.997
EEDS dosis 100 mg/kg
BB
.153
5
.200
*
.994
5
.993
EEDS dosis 200 mg/kg
BB
.183
5
.200
*
.949
5
.729
EEDS dosis 400 mg/kg
BB
.139
5
.200
*
.991
5
.983
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
Waktu Awal Diare
95% Confidence
Interval for Mean
N
Suspensi Na-CMC
0,5%
Suspensi Loperamid
0,52 mg/kg BB
EEDS dosis 50
mg/kg BB
EEDS dosis 100
mg/kg BB
EEDS dosis 200
mg/kg BB
EEDS dosis 400
mg/kg BB
Total
Mean
Std.
Deviation
Std.
Error
Lower
Bound
5
84.00
7.314
3.271
5
139.20
12.677
5
93.60
5
Upper
Bound Minimum Maximum
74.92
93.08
75
94
5.669
123.46 154.94
124
156
8.503
3.803
83.04 104.16
82
105
106.20
6.058
2.709
98.68 113.72
98
114
5
118.40
7.162
3.203
109.51 127.29
110
127
5
129.80
9.680
4.329
117.78 141.82
117
142
30
111.87
21.287
3.887
103.92 119.82
75
156
ANOVA
Waktu Awal Diare
Sum of
Squares
df
Mean Square
Between Groups
Within Groups
11268.667
1872.800
5
24
Total
13141.467
29
2253.733
78.033
F
28.882
Sig.
.000
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Lampiran 18. (Lanjutan)
Multiple Comparisons
Waktu Awal Diare
Tukey HSD
95% Confidence Interval
(I) Kelompok
Suspensi NaCMC 0,5%
(J) Kelompok
Mean Difference
(I-J)
Std. Error
Suspensi Loperamid 0,52
mg/kg BB
-55.200
EEDS dosis 50 mg/kg BB
Lower
Bound
Sig.
Upper Bound
*
5.587
.000
-72.47
-37.93
-9.600
5.587
.534
-26.87
7.67
EEDS dosis 100 mg/kg BB
-22.200*
5.587
.007
-39.47
-4.93
EEDS dosis 200 mg/kg BB
-34.400*
5.587
.000
-51.67
-17.13
EEDS dosis 400 mg/kg BB
-45.800*
5.587
.000
-63.07
-28.53
Suspensi
Suspensi Na-CMC 0,5%
Loperamid
EEDS dosis 50 mg/kg BB
0,52 mg/kg BB
EEDS dosis 100 mg/kg BB
55.200
*
5.587
.000
37.93
72.47
45.600*
5.587
.000
28.33
62.87
33.000*
5.587
.000
15.73
50.27
EEDS dosis 200 mg/kg BB
20.800*
5.587
.012
3.53
38.07
EEDS dosis 400 mg/kg BB
9.400
5.587
.556
-7.87
26.67
9.600
5.587
.534
-7.67
26.87
-45.600*
5.587
.000
-62.87
-28.33
EEDS dosis 100 mg/kg BB
-12.600
5.587
.251
-29.87
4.67
EEDS dosis 200 mg/kg BB
-24.800*
5.587
.002
-42.07
-7.53
EEDS dosis 400 mg/kg BB
-36.200*
5.587
.000
-53.47
-18.93
22.200*
5.587
.007
4.93
39.47
-33.000*
5.587
.000
-50.27
-15.73
EEDS dosis 50 mg/kg BB
12.600
5.587
.251
-4.67
29.87
EEDS dosis 200 mg/kg BB
-12.200
5.587
.281
-29.47
5.07
EEDS dosis 400 mg/kg BB
*
EEDS dosis 50 Suspensi Na-CMC 0,5%
mg/kg BB
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis
Suspensi Na-CMC 0,5%
100 mg/kg BB
Suspensi Loperamid 0,52
mg/kg BB
5.587
.004
-40.87
-6.33
EEDS dosis
Suspensi Na-CMC 0,5%
200 mg/kg BB Suspensi Loperamid 0,52
mg/kg BB
-23.600
34.400*
5.587
.000
17.13
51.67
-20.800*
5.587
.012
-38.07
-3.53
EEDS dosis 50 mg/kg BB
24.800*
5.587
.002
7.53
42.07
EEDS dosis 100 mg/kg BB
12.200
5.587
.281
-5.07
29.47
EEDS dosis 400 mg/kg BB
-11.400
5.587
.350
-28.67
5.87
*
EEDS dosis
Suspensi Na-CMC 0,5%
400 mg/kg BB
Suspensi Loperamid 0,52
mg/kg BB
45.800
5.587
.000
28.53
63.07
-9.400
5.587
.556
-26.67
7.87
EEDS dosis 50 mg/kg BB
36.200*
5.587
.000
18.93
53.47
EEDS dosis 100 mg/kg BB
23.600*
5.587
.004
6.33
40.87
EEDS dosis 200 mg/kg BB
11.400
5.587
.350
-5.87
28.67
Waktu Awal Diare
Tukey HSD
a
Subset for alpha = 0.05
Kelompok
N
1
2
Suspensi Na-CMC 0,5%
5
84.00
EEDS dosis 50 mg/kg BB
5
93.60
EEDS dosis 100 mg/kg BB
5
EEDS dosis 200 mg/kg BB
5
EEDS dosis 400 mg/kg BB
5
Suspensi Loperamid 0,52 mg/kg BB
5
3
4
5
93.60
106.20
106.20
118.40
118.40
129.80
129.80
139.20
Sig.
.534
.251
.281
.350
.556
87
Universitas Sumatera Utara
Lampiran 19. Analisis statistik frekuensi diare
Tests of Normality
a
Kolmogorov-Smirnov
Kelompok
Frekuensi
Diare
Statistic
Suspensi Na-CMC 0,5%
df
.224
Shapiro-Wilk
Sig.
5
Statistic
df
Sig.
.200
*
.842
5
.171
.961
5
.814
Suspensi Loperamid
0,52 mg/kg BB
.237
5
.200
*
EEDS dosis 50 mg/kg
BB
.241
5
.200
*
.821
5
.119
EEDS dosis 100 mg/kg
BB
.237
5
.200
*
.961
5
.814
EEDS dosis 200 mg/kg
BB
.237
5
.200
*
.961
5
.814
EEDS dosis 400 mg/kg
BB
.221
5
.200
*
.902
5
.421
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
Frekuensi Diare
95% Confidence
Interval for
Mean
Std.
Mean Deviation
N
Suspensi Na-CMC
0,5%
Suspensi Loperamid
0,52 mg/kg BB
EEDS dosis 50 mg/kg
BB
EEDS dosis 100 mg/kg
BB
EEDS dosis 200 mg/kg
BB
EEDS dosis 400 mg/kg
BB
Total
Std.
Error
Lower Upper
Bound Bound Minimum Maximum
5
11.60
2.074
.927
9.03
14.17
10
15
5
4.60
1.140
.510
3.18
6.02
3
6
5
10.00
1.000
.447
8.76
11.24
9
11
5
8.60
1.140
.510
7.18
10.02
7
10
5
7.40
1.140
.510
5.98
8.82
6
9
5
6.80
1.304
.583
5.18
8.42
5
8
30
8.17
2.601
.475
7.20
9.14
3
15
ANOVA
Frekuensi Diare
Sum of
Squares
Df
Mean Square
Between Groups
Within Groups
152.567
43.600
5
24
Total
196.167
29
30.513
1.817
F
16.796
Sig.
.000
88
Universitas Sumatera Utara
Lampiran 19. (Lanjutan)
Multiple Comparisons
Frekuensi Diare
Tukey HSD
(I) Kelompok
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 50 mg/kg BB
Mean
Difference (I-J) Std. Error
(J) Kelompok
95% Confidence Interval
Sig.
Lower Bound
Upper Bound
*
7.000
.852
.000
4.36
9.64
EEDS dosis 50 mg/kg BB
1.600
.852
.439
-1.04
4.24
EEDS dosis 100 mg/kg BB
3.000
*
.852
.019
.36
5.64
*
6.84
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 200 mg/kg BB
4.200
.852
.001
1.56
EEDS dosis 400 mg/kg BB
4.800
*
.852
.000
2.16
7.44
Suspensi Na-CMC 0,5%
-7.000*
.852
.000
-9.64
-4.36
EEDS dosis 50 mg/kg BB
-5.400*
.852
.000
-8.04
-2.76
EEDS dosis 100 mg/kg BB
-4.000*
.852
.001
-6.64
-1.36
EEDS dosis 200 mg/kg BB
-2.800*
.852
.033
-5.44
-.16
EEDS dosis 400 mg/kg BB
-2.200
.852
.141
-4.84
.44
Suspensi Na-CMC 0,5%
-1.600
.852
.439
-4.24
1.04
Suspensi Loperamid 0,52
mg/kg BB
5.400
*
.852
.000
2.76
8.04
EEDS dosis 100 mg/kg BB
1.400
.852
.580
-1.24
4.04
EEDS dosis 200 mg/kg BB
2.600
.852
.055
-.04
5.24
EEDS dosis 400 mg/kg BB
3.200
*
.852
.011
.56
5.84
*
.852
.019
-5.64
-.36
EEDS dosis 100 mg/kg BB Suspensi Na-CMC 0,5%
-3.000
Suspensi Loperamid 0,52
mg/kg BB
4.000
*
.852
.001
1.36
6.64
EEDS dosis 50 mg/kg BB
-1.400
.852
.580
-4.04
1.24
EEDS dosis 200 mg/kg BB
1.200
.852
.722
-1.44
3.84
EEDS dosis 400 mg/kg BB
1.800
.852
.315
-.84
4.44
*
.852
.001
-6.84
-1.56
5.44
EEDS dosis 200 mg/kg BB Suspensi Na-CMC 0,5%
-4.200
Suspensi Loperamid 0,52
mg/kg BB
2.800
*
.852
.033
.16
EEDS dosis 50 mg/kg BB
-2.600
.852
.055
-5.24
.04
EEDS dosis 100 mg/kg BB
-1.200
.852
.722
-3.84
1.44
EEDS dosis 400 mg/kg BB
.600
.852
.980
-2.04
3.24
-4.800*
.852
.000
-7.44
-2.16
Suspensi Loperamid 0,52
mg/kg BB
2.200
.852
.141
-.44
4.84
EEDS dosis 50 mg/kg BB
-3.200
*
.852
.011
-5.84
-.56
EEDS dosis 100 mg/kg BB
-1.800
.852
.315
-4.44
.84
EEDS dosis 200 mg/kg BB
-.600
.852
.980
-3.24
2.04
EEDS dosis 400 mg/kg BB Suspensi Na-CMC 0,5%
*. The mean difference is significant at the 0.05 level.
Frekuensi Diare
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Suspensi Loperamid 0,52 mg/kg BB
5
4.60
EEDS dosis 400 mg/kg BB
5
6.80
EEDS dosis 200 mg/kg BB
5
7.40
EEDS dosis 100 mg/kg BB
5
8.60
EEDS dosis 50 mg/kg BB
5
Suspensi Na-CMC 0,5%
5
4
6.80
7.40
8.60
10.00
10.00
11.60
Sig.
.141
.315
.055
.439
89
Universitas Sumatera Utara
Lampiran 20. Analisis statistik konsistensi dan berat feses padat
Tests of Normality
a
Kolmogorov-Smirnov
Kelompok
Berat
Feses
Padat
Statistic
df
Shapiro-Wilk
Sig.
Statistic
df
Sig.
Suspensi Na-CMC
0,5%
.197
5
.200
*
.935
5
.631
Suspensi Loperamid
0,52 mg/kg BB
.215
5
.200
*
.937
5
.642
EEDS dosis 50 mg/kg
BB
.200
5
.200
*
.948
5
.723
EEDS dosis 100
mg/kg BB
.230
5
.200
*
.955
5
.774
EEDS dosis 200
mg/kg BB
.202
5
.200
*
.952
5
.752
EEDS dosis 400
mg/kg BB
.142
5
.200
*
.996
5
.995
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
Berat Feses Padat
95% Confidence
Interval for Mean
Std.
Mean Deviation
N
Suspensi Na-CMC
0,5%
Suspensi Loperamid
0,52 mg/kg BB
EEDS dosis 50 mg/kg
BB
EEDS dosis 100 mg/kg
BB
EEDS dosis 200 mg/kg
BB
EEDS dosis 400 mg/kg
BB
Total
Std.
Error
Lower
Bound
Upper
Bound Minimum Maximum
5
.4220
.12276 .05490
.2696
.5744
.24
.55
5
.1520
.05805 .02596
.0799
.2241
.09
.23
5
.3760
.10237 .04578
.2489
.5031
.27
.53
5
.2880
.12518 .05598
.1326
.4434
.12
.47
5
.2620
.14394 .06437
.0833
.4407
.10
.45
5
.2080
.07629 .03412
.1133
.3027
.11
.31
30
.2847
.13653 .02493
.2337
.3356
.09
.55
ANOVA
Berat Feses Padat
Sum of
Squares
df
Mean Square
Between Groups
Within Groups
.256
.285
5
24
Total
.541
29
.051
.012
F
Sig.
4.319
.006
90
Universitas Sumatera Utara
Lampiran 20. (Lanjutan)
Multiple Comparisons
Berat Feses Padat
Tukey HSD
Mean
Difference (I-J) Std. Error
95% Confidence Interval
(I) Kelompok
(J) Kelompok
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
.27000
*
.06886
.007
.0571
.4829
EEDS dosis 50 mg/kg BB
.04600
.06886
.984
-.1669
.2589
EEDS dosis 100 mg/kg BB
.13400
.06886
.400
-.0789
.3469
EEDS dosis 200 mg/kg BB
.16000
.06886
.224
-.0529
.3729
EEDS dosis 400 mg/kg BB
.21400
*
.06886
.048
.0011
.4269
*
.06886
.007
-.4829
-.0571
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 50 mg/kg BB
Sig.
Lower Bound
Upper Bound
Suspensi Na-CMC 0,5%
-.27000
EEDS dosis 50 mg/kg BB
-.22400
*
.06886
.035
-.4369
-.0111
EEDS dosis 100 mg/kg BB
-.13600
.06886
.385
-.3489
.0769
EEDS dosis 200 mg/kg BB
-.11000
.06886
.608
-.3229
.1029
EEDS dosis 400 mg/kg BB
-.05600
.06886
.962
-.2689
.1569
Suspensi Na-CMC 0,5%
-.04600
.06886
.984
-.2589
.1669
Suspensi Loperamid 0,52
mg/kg BB
.22400
*
.06886
.035
.0111
.4369
EEDS dosis 100 mg/kg BB
.08800
.06886
.794
-.1249
.3009
EEDS dosis 200 mg/kg BB
.11400
.06886
.572
-.0989
.3269
EEDS dosis 400 mg/kg BB
.16800
.06886
.183
-.0449
.3809
-.13400
.06886
.400
-.3469
.0789
Suspensi Loperamid 0,52
mg/kg BB
.13600
.06886
.385
-.0769
.3489
EEDS dosis 50 mg/kg BB
-.08800
.06886
.794
-.3009
.1249
EEDS dosis 200 mg/kg BB
.02600
.06886
.999
-.1869
.2389
EEDS dosis 400 mg/kg BB
.08000
.06886
.850
-.1329
.2929
-.16000
.06886
.224
-.3729
.0529
Suspensi Loperamid 0,52
mg/kg BB
.11000
.06886
.608
-.1029
.3229
EEDS dosis 50 mg/kg BB
-.11400
.06886
.572
-.3269
.0989
EEDS dosis 100 mg/kg BB
-.02600
.06886
.999
-.2389
.1869
EEDS dosis 400 mg/kg BB
.05400
.06886
.968
-.1589
.2669
-.21400*
.06886
.048
-.4269
-.0011
Suspensi Loperamid 0,52
mg/kg BB
.05600
.06886
.962
-.1569
.2689
EEDS dosis 50 mg/kg BB
-.16800
.06886
.183
-.3809
.0449
EEDS dosis 100 mg/kg BB
-.08000
.06886
.850
-.2929
.1329
EEDS dosis 200 mg/kg BB
-.05400
.06886
.968
-.2669
.1589
EEDS dosis 100 mg/kg BB Suspensi Na-CMC 0,5%
EEDS dosis 200 mg/kg BB Suspensi Na-CMC 0,5%
EEDS dosis 400 mg/kg BB Suspensi Na-CMC 0,5%
*. The mean difference is significant at the 0.05 level.
Berat Feses Padat
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Suspensi Loperamid 0,52 mg/kg BB
5
.1520
EEDS dosis 400 mg/kg BB
5
.2080
.2080
EEDS dosis 200 mg/kg BB
5
.2620
.2620
.2620
EEDS dosis 100 mg/kg BB
5
.2880
.2880
.2880
EEDS dosis 50 mg/kg BB
5
.3760
.3760
Suspensi Na-CMC 0,5%
5
.4220
Sig.
.385
.183
.224
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
91
Universitas Sumatera Utara
Lampiran 21. Analisis statistik konsistensi dan berat feses berair
Tests of Normality
a
Kolmogorov-Smirnov
Kelompok
Berat Feses
Berair
Statistic
df
Shapiro-Wilk
Sig.
Statistic
df
Sig.
Suspensi Na-CMC
0,5%
.223
5
.200
*
Suspensi Loperamid
0,52 mg/kg BB
.221
5
.200
*
.915
5
.501
EEDS dosis 50 mg/kg
BB
.155
5
.200
*
.976
5
.911
EEDS dosis 100
mg/kg BB
.231
5
.200
*
.945
5
.699
EEDS dosis 200
mg/kg BB
.189
5
.200
*
.952
5
.750
EEDS dosis 400
mg/kg BB
.234
5
.200
*
.950
5
.735
.929
5
.591
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
Berat Feses Berair
95%
Confidence
Interval for
Mean
Std.
Mean Deviation
N
Suspensi Na-CMC 0,5%
Suspensi Loperamid
0,52 mg/kg BB
EEDS dosis 50 mg/kg
BB
EEDS dosis 100 mg/kg
BB
EEDS dosis 200 mg/kg
BB
EEDS dosis 400 mg/kg
BB
Total
Std.
Error
Lower Upper
Bound Bound Minimum Maximum
5
5
.4580
.1740
.14446
.05177
.06461
.02315
.2786
.1097
.6374
.2383
.23
.11
.62
.23
5
.3620
.08408
.03760
.2576
.4664
.25
.46
5
.2640
.08019
.03586
.1644
.3636
.14
.36
5
.2260
.07956
.03558
.1272
.3248
.13
.32
5
.2220
.10756
.04810
.0884
.3556
.10
.39
30
.2843
.13132
.02398
.2353
.3334
.10
.62
ANOVA
Berat Feses Berair
Sum of Squares
df
Mean Square
Between Groups
Within Groups
.280
.220
5
24
Total
.500
29
.056
.009
F
Sig.
6.122
.001
92
Universitas Sumatera Utara
Lampiran 21. (Lanjutan)
Multiple Comparisons
Berat Feses Berair
Tukey HSD
(I) Kelompok
Suspensi Na-CMC 0,5%
Mean
Difference (I-J) Std. Error
(J) Kelompok
EEDS dosis 50 mg/kg BB
Lower Bound
Upper Bound
*
.28400
.06053
.001
.0969
.4711
EEDS dosis 50 mg/kg BB
.09600
.06053
.615
-.0911
.2831
EEDS dosis 100 mg/kg BB
.19400
*
.06053
.039
.0069
.3811
EEDS dosis 200 mg/kg BB
.23200
*
.06053
.009
.0449
.4191
*
.06053
.008
.0489
.4231
*
.06053
.001
-.4711
-.0969
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 400 mg/kg BB
Suspensi Loperamid 0,52
mg/kg BB
95% Confidence Interval
Sig.
.23600
Suspensi Na-CMC 0,5%
-.28400
EEDS dosis 50 mg/kg BB
-.18800
*
.06053
.048
-.3751
-.0009
EEDS dosis 100 mg/kg BB
-.09000
.06053
.675
-.2771
.0971
EEDS dosis 200 mg/kg BB
-.05200
.06053
.953
-.2391
.1351
EEDS dosis 400 mg/kg BB
-.04800
.06053
.966
-.2351
.1391
Suspensi Na-CMC 0,5%
-.09600
.06053
.615
-.2831
.0911
Suspensi Loperamid 0,52
mg/kg BB
.18800
*
.06053
.048
.0009
.3751
EEDS dosis 100 mg/kg BB
.09800
.06053
.595
-.0891
.2851
EEDS dosis 200 mg/kg BB
.13600
.06053
.254
-.0511
.3231
EEDS dosis 400 mg/kg BB
.14000
.06053
.228
-.0471
.3271
-.19400
*
.06053
.039
-.3811
-.0069
Suspensi Loperamid 0,52
mg/kg BB
.09000
.06053
.675
-.0971
.2771
EEDS dosis 50 mg/kg BB
-.09800
.06053
.595
-.2851
.0891
EEDS dosis 200 mg/kg BB
.03800
.06053
.988
-.1491
.2251
EEDS dosis 400 mg/kg BB
.04200
.06053
.981
-.1451
.2291
-.23200
*
.06053
.009
-.4191
-.0449
Suspensi Loperamid 0,52
mg/kg BB
.05200
.06053
.953
-.1351
.2391
EEDS dosis 50 mg/kg BB
-.13600
.06053
.254
-.3231
.0511
EEDS dosis 100 mg/kg BB
-.03800
.06053
.988
-.2251
.1491
EEDS dosis 400 mg/kg BB
.00400
.06053
1.000
-.1831
.1911
-.23600
*
.06053
.008
-.4231
-.0489
Suspensi Loperamid 0,52
mg/kg BB
.04800
.06053
.966
-.1391
.2351
EEDS dosis 50 mg/kg BB
-.14000
.06053
.228
-.3271
.0471
EEDS dosis 100 mg/kg BB
-.04200
.06053
.981
-.2291
.1451
EEDS dosis 200 mg/kg BB
-.00400
.06053
1.000
-.1911
.1831
EEDS dosis 100 mg/kg BB Suspensi Na-CMC 0,5%
EEDS dosis 200 mg/kg BB Suspensi Na-CMC 0,5%
EEDS dosis 400 mg/kg BB Suspensi Na-CMC 0,5%
*. The mean difference is significant at the 0.05 level.
Berat Feses Berair
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Suspensi Loperamid 0,52 mg/kg BB
5
.1740
EEDS dosis 400 mg/kg BB
5
.2220
.2220
EEDS dosis 200 mg/kg BB
5
.2260
.2260
EEDS dosis 100 mg/kg BB
5
.2640
.2640
EEDS dosis 50 mg/kg BB
5
Suspensi Na-CMC 0,5%
5
.3620
.3620
.4580
Sig.
.675
.228
.615
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
93
Universitas Sumatera Utara
Lampiran 22. Analisis statistik konsistensi dan berat feses lembek
Tests of Normality
a
Kolmogorov-Smirnov
Kelompok
Berat
Feses
Lembek
Statistic
Suspensi Na-CMC 0,5%
df
Shapiro-Wilk
Sig.
.201
5
Statistic
df
Sig.
.200
*
.951
5
.747
.884
5
.330
Suspensi Loperamid
0,52 mg/kg BB
.221
5
.200
*
EEDS dosis 50 mg/kg
BB
.282
5
.200
*
.868
5
.259
EEDS dosis 100 mg/kg
BB
.251
5
.200
*
.932
5
.611
EEDS dosis 200 mg/kg
BB
.237
5
.200
*
.908
5
.455
EEDS dosis 400 mg/kg
BB
.239
5
.200
*
.861
5
.230
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
Berat Feses Lembek
95%
Confidence
Interval for
Mean
Std.
Mean Deviation
N
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 50 mg/kg BB
EEDS dosis 100 mg/kg
BB
EEDS dosis 200 mg/kg
BB
EEDS dosis 400 mg/kg
BB
Total
Std.
Error
Lower Upper
Bound Bound Minimum Maximum
5 1.3800
5 .5800
.23969
.12942
.10719 1.0824 1.6776
.05788 .4193 .7407
1.02
.46
1.64
.75
5 1.2620
5 .9080
.27068
.27905
.12105 .9259 1.5981
.12480 .5615 1.2545
.90
.58
1.51
1.26
5
.7840
.19970
.08931 .5360 1.0320
.53
.99
5
.6640
.21813
.09755 .3932 .9348
.43
.89
30
.9297
.36625
.06687 .7929 1.0664
.43
1.64
ANOVA
Berat Feses Lembek
Sum of
Squares
Mean
Square
df
Between Groups
Within Groups
2.639
1.251
5
24
Total
3.890
29
F
.528
.052
10.124
Sig.
.000
94
Universitas Sumatera Utara
Lampiran 22. (Lanjutan)
Multiple Comparisons
Berat Feses Lembek
Tukey HSD
(I) Kelompok
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 50 mg/kg BB
95% Confidence Interval
Mean
Difference (I-J) Std. Error
(J) Kelompok
Sig.
Lower Bound
Upper Bound
*
.80000
.14441
.000
.3535
1.2465
EEDS dosis 50 mg/kg BB
.11800
.14441
.961
-.3285
.5645
EEDS dosis 100 mg/kg BB
.47200
*
.14441
.034
.0255
.9185
EEDS dosis 200 mg/kg BB
.59600
*
.14441
.005
.1495
1.0425
EEDS dosis 400 mg/kg BB
.71600*
.14441
.001
.2695
1.1625
Suspensi Na-CMC 0,5%
-.80000*
.14441
.000
-1.2465
-.3535
EEDS dosis 50 mg/kg BB
-.68200*
.14441
.001
-1.1285
-.2355
EEDS dosis 100 mg/kg BB
-.32800
.14441
.244
-.7745
.1185
EEDS dosis 200 mg/kg BB
-.20400
.14441
.719
-.6505
.2425
EEDS dosis 400 mg/kg BB
-.08400
.14441
.991
-.5305
.3625
Suspensi Na-CMC 0,5%
-.11800
.14441
.961
-.5645
.3285
Suspensi Loperamid 0,52
mg/kg BB
.68200
*
.14441
.001
.2355
1.1285
EEDS dosis 100 mg/kg BB
.35400
.14441
.179
-.0925
.8005
EEDS dosis 200 mg/kg BB
.47800
*
.14441
.031
.0315
.9245
EEDS dosis 400 mg/kg BB
.59800
*
.14441
.004
.1515
1.0445
-.47200
*
.14441
.034
-.9185
-.0255
Suspensi Loperamid 0,52
mg/kg BB
.32800
.14441
.244
-.1185
.7745
EEDS dosis 50 mg/kg BB
-.35400
.14441
.179
-.8005
.0925
EEDS dosis 200 mg/kg BB
.12400
.14441
.953
-.3225
.5705
EEDS dosis 400 mg/kg BB
.24400
.14441
.551
-.2025
.6905
-.59600
*
.14441
.005
-1.0425
-.1495
Suspensi Loperamid 0,52
mg/kg BB
.20400
.14441
.719
-.2425
.6505
EEDS dosis 50 mg/kg BB
-.47800*
.14441
.031
-.9245
-.0315
EEDS dosis 100 mg/kg BB
-.12400
.14441
.953
-.5705
.3225
EEDS dosis 400 mg/kg BB
.12000
.14441
.959
-.3265
.5665
-.71600
*
.14441
.001
-1.1625
-.2695
Suspensi Loperamid 0,52
mg/kg BB
.08400
.14441
.991
-.3625
.5305
EEDS dosis 50 mg/kg BB
-.59800
*
.14441
.004
-1.0445
-.1515
EEDS dosis 100 mg/kg BB
-.24400
.14441
.551
-.6905
.2025
EEDS dosis 200 mg/kg BB
-.12000
.14441
.959
-.5665
.3265
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 100 mg/kg BB Suspensi Na-CMC 0,5%
EEDS dosis 200 mg/kg BB Suspensi Na-CMC 0,5%
EEDS dosis 400 mg/kg BB Suspensi Na-CMC 0,5%
*. The mean difference is significant at the 0.05 level.
Berat Feses Lembek
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
Suspensi Loperamid 0,52 mg/kg BB
5
.5800
EEDS dosis 400 mg/kg BB
5
.6640
EEDS dosis 200 mg/kg BB
5
.7840
EEDS dosis 100 mg/kg BB
5
.9080
EEDS dosis 50 mg/kg BB
5
Suspensi Na-CMC 0,5%
5
Sig.
3
.9080
1.2620
1.2620
1.3800
.244
.179
.961
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
95
Universitas Sumatera Utara
Lampiran 23. Analisis statistik durasi diare
Tests of Normality
a
Kolmogorov-Smirnov
Kelompok
Lama
Diare
Statistic
Suspensi Na-CMC 0,5%
df
Shapiro-Wilk
Sig.
.268
5
Statistic
df
Sig.
.200
*
.845
5
.178
.905
5
.437
Suspensi Loperamid 0,52
mg/kg BB
.284
5
.200
*
EEDS dosis 50 mg/kg BB
.209
5
.200
*
.943
5
.688
*
.908
5
.457
EEDS dosis 100 mg/kg BB
.233
5
.200
EEDS dosis 200 mg/kg BB
.302
5
.155
.857
5
.219
5
*
.991
5
.982
EEDS dosis 400 mg/kg BB
.139
.200
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
Lama Diare
95% Confidence
Interval for Mean
N
Suspensi Na-CMC
0,5%
Suspensi Loperamid
0,52 mg/kg BB
EEDS dosis 50 mg/kg
BB
EEDS dosis 100
mg/kg BB
EEDS dosis 200
mg/kg BB
EEDS dosis 400
mg/kg BB
Total
Mean
Std.
Deviation
5
198.00
12.490
5
87.40
9.209
5
169.60
5
Std.
Error
Lower
Bound
5.586 182.49
Upper
Bound Minimum Maximum
213.51
187
219
75.97
98.83
78
100
10.761
4.812 156.24
182.96
155
181
149.40
4.722
2.112 143.54
155.26
144
155
5
131.40
6.427
2.874 123.42
139.38
126
141
5
110.60
14.293
6.392
92.85
128.35
91
128
30
141.07
38.341
7.000 126.75
155.38
78
219
4.118
ANOVA
Lama Diare
Sum of Squares
df
Mean Square
Between Groups
Within Groups
40133.867
2498.000
5
24
Total
42631.867
29
8026.773
104.083
F
77.119
Sig.
.000
Lampiran 23. (Lanjutan)
96
Universitas Sumatera Utara
Multiple Comparisons
Lama Diare
Tukey HSD
(I) Kelompok
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 50 mg/kg BB
Mean
Difference (I-J) Std. Error
(J) Kelompok
Lower Bound
*
6.452
.000
*
Suspensi Loperamid 0,52
mg/kg BB
110.600
EEDS dosis 50 mg/kg BB
28.400
6.452
EEDS dosis 100 mg/kg BB
48.600
*
6.452
EEDS dosis 200 mg/kg BB
66.600*
EEDS dosis 400 mg/kg BB
Upper Bound
90.65
130.55
.002
8.45
48.35
.000
28.65
68.55
6.452
.000
46.65
86.55
*
6.452
.000
67.45
107.35
*
6.452
.000
-130.55
-90.65
*
6.452
.000
-102.15
-62.25
*
6.452
.000
-81.95
-42.05
*
6.452
.000
-63.95
-24.05
*
6.452
.016
-43.15
-3.25
*
6.452
.002
-48.35
-8.45
*
6.452
.000
62.25
102.15
*
6.452
.046
.25
40.15
*
6.452
.000
18.25
58.15
*
6.452
.000
39.05
78.95
*
6.452
.000
-68.55
-28.65
*
6.452
.000
42.05
81.95
87.400
Suspensi Na-CMC 0,5%
-110.600
EEDS dosis 50 mg/kg BB
-82.200
EEDS dosis 100 mg/kg BB
-62.000
EEDS dosis 200 mg/kg BB
-44.000
EEDS dosis 400 mg/kg BB
-23.200
Suspensi Na-CMC 0,5%
-28.400
Suspensi Loperamid 0,52
mg/kg BB
82.200
EEDS dosis 100 mg/kg BB
20.200
EEDS dosis 200 mg/kg BB
38.200
EEDS dosis 400 mg/kg BB
59.000
EEDS dosis 100 mg/kg BB Suspensi Na-CMC 0,5%
95% Confidence Interval
Sig.
-48.600
Suspensi Loperamid 0,52
mg/kg BB
62.000
EEDS dosis 50 mg/kg BB
-20.200
*
6.452
.046
-40.15
-.25
EEDS dosis 200 mg/kg BB
18.000
6.452
.094
-1.95
37.95
EEDS dosis 400 mg/kg BB
38.800
*
6.452
.000
18.85
58.75
*
6.452
.000
-86.55
-46.65
*
6.452
.000
24.05
63.95
-18.25
EEDS dosis 200 mg/kg BB Suspensi Na-CMC 0,5%
-66.600
Suspensi Loperamid 0,52
mg/kg BB
44.000
EEDS dosis 50 mg/kg BB
-38.200
*
6.452
.000
-58.15
EEDS dosis 100 mg/kg BB
-18.000
6.452
.094
-37.95
1.95
EEDS dosis 400 mg/kg BB
20.800
*
6.452
.038
.85
40.75
EEDS dosis 400 mg/kg BB Suspensi Na-CMC 0,5%
*
6.452
.000
-107.35
-67.45
*
6.452
.016
3.25
43.15
*
6.452
.000
-78.95
-39.05
*
6.452
.000
-58.75
-18.85
*
6.452
.038
-40.75
-.85
-87.400
Suspensi Loperamid 0,52
mg/kg BB
23.200
EEDS dosis 50 mg/kg BB
-59.000
EEDS dosis 100 mg/kg BB
-38.800
EEDS dosis 200 mg/kg BB
-20.800
*. The mean difference is significant at the 0.05 level.
Lama Diare
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
4
Suspensi Loperamid 0,52 mg/kg BB
5
EEDS dosis 400 mg/kg BB
5
EEDS dosis 200 mg/kg BB
5
131.40
EEDS dosis 100 mg/kg BB
5
149.40
EEDS dosis 50 mg/kg BB
5
Suspensi Na-CMC 0,5%
5
Sig.
5
87.40
110.60
169.60
198.00
1.000
1.000
.094
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
97
Universitas Sumatera Utara
Lampiran 24. Analisis statistik persentase lintas marker
Tests of Normality
a
Kolmogorov-Smirnov
Kelompok
Persen
Lintasan
Statistic
df
Shapiro-Wilk
Sig.
Statistic
df
Sig.
Suspensi Na-CMC
0,5%
.239
5
.200
*
Suspensi Loperamid
0,52 mg/kg BB
.211
5
.200
*
.919
5
.521
EEDS dosis 50 mg/kg
BB
.301
5
.157
.862
5
.235
EEDS dosis 100 mg/kg
BB
.220
5
.200
*
.960
5
.808
EEDS dosis 200 mg/kg
BB
.234
5
.200
*
.933
5
.617
EEDS dosis 400 mg/kg
BB
.336
5
.068
.762
5
.038
.832
5
.145
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
Persen Lintasan
95% Confidence
Interval for Mean
N
Suspensi Na-CMC
0,5%
Suspensi Loperamid
0,52 mg/kg BB
EEDS dosis 50 mg/kg
BB
EEDS dosis 100
mg/kg BB
EEDS dosis 200
mg/kg BB
EEDS dosis 400
mg/kg BB
Total
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound Minimum Maximum
5 89.1100
5.71963 2.55790 82.0081 96.2119
79.62
93.47
5 45.5240
10.58572 4.73408 32.3801 58.6679
32.92
57.37
5 86.1840
5.70351 2.55069 79.1022 93.2658
76.61
91.48
5 66.4340
4.01254 1.79446 61.4518 71.4162
61.95
72.11
5 56.3720
9.31079 4.16391 44.8111 67.9329
45.83
68.18
5 55.8420
9.23095 4.12821 44.3803 67.3037
39.78
62.50
30 66.5777
17.84534 3.25810 59.9141 73.2412
32.92
93.47
ANOVA
Persen Lintasan
Sum of Squares
df
Mean Square
Between Groups
Within Groups
7774.010
1461.214
5
24
Total
9235.225
29
1554.802
60.884
F
25.537
Sig.
.000
98
Universitas Sumatera Utara
Lampiran 24. (Lanjutan)
Multiple Comparisons
Persen Lintasan
Tukey HSD
Mean
Difference (I-J) Std. Error
(I) Kelompok
(J) Kelompok
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
Lower Bound
Upper Bound
*
4.93493
.000
28.3275
58.8445
2.92600
4.93493
.991
-12.3325
18.1845
*
4.93493
.001
7.4175
37.9345
EEDS dosis 200 mg/kg BB
*
32.73800
4.93493
.000
17.4795
47.9965
EEDS dosis 400 mg/kg BB
33.26800*
4.93493
.000
18.0095
48.5265
Suspensi Na-CMC 0,5%
-43.58600*
4.93493
.000
-58.8445
-28.3275
EEDS dosis 50 mg/kg BB
-40.66000*
4.93493
.000
-55.9185
-25.4015
EEDS dosis 100 mg/kg BB
-20.91000
*
4.93493
.003
-36.1685
-5.6515
EEDS dosis 200 mg/kg BB
-10.84800
4.93493
.275
-26.1065
4.4105
EEDS dosis 400 mg/kg BB
-10.31800
4.93493
.325
-25.5765
4.9405
-2.92600
4.93493
.991
-18.1845
12.3325
*
4.93493
.000
25.4015
55.9185
*
4.93493
.006
4.4915
35.0085
*
4.93493
.000
14.5535
45.0705
*
4.93493
.000
15.0835
45.6005
*
4.93493
.001
-37.9345
-7.4175
36.1685
EEDS dosis 100 mg/kg BB
EEDS dosis 50 mg/kg BB
Sig.
43.58600
EEDS dosis 50 mg/kg BB
Suspensi Loperamid 0,52
mg/kg BB
95% Confidence Interval
22.67600
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
40.66000
EEDS dosis 100 mg/kg BB
19.75000
EEDS dosis 200 mg/kg BB
29.81200
EEDS dosis 400 mg/kg BB
30.34200
EEDS dosis 100 mg/kg BB Suspensi Na-CMC 0,5%
-22.67600
Suspensi Loperamid 0,52
mg/kg BB
20.91000
*
4.93493
.003
5.6515
EEDS dosis 50 mg/kg BB
-19.75000*
4.93493
.006
-35.0085
-4.4915
EEDS dosis 200 mg/kg BB
10.06200
4.93493
.351
-5.1965
25.3205
10.59200
4.93493
.298
-4.6665
25.8505
*
4.93493
.000
-47.9965
-17.4795
Suspensi Loperamid 0,52
mg/kg BB
10.84800
4.93493
.275
-4.4105
26.1065
EEDS dosis 50 mg/kg BB
-29.81200*
4.93493
.000
-45.0705
-14.5535
EEDS dosis 100 mg/kg BB
-10.06200
4.93493
.351
-25.3205
5.1965
EEDS dosis 400 mg/kg BB
.53000
4.93493
1.000
-14.7285
15.7885
*
4.93493
.000
-48.5265
-18.0095
Suspensi Loperamid 0,52
mg/kg BB
10.31800
4.93493
.325
-4.9405
25.5765
EEDS dosis 50 mg/kg BB
-30.34200
*
4.93493
.000
-45.6005
-15.0835
EEDS dosis 100 mg/kg BB
-10.59200
4.93493
.298
-25.8505
4.6665
EEDS dosis 200 mg/kg BB
-.53000
4.93493
1.000
-15.7885
14.7285
EEDS dosis 400 mg/kg BB
EEDS dosis 200 mg/kg BB Suspensi Na-CMC 0,5%
-32.73800
EEDS dosis 400 mg/kg BB Suspensi Na-CMC 0,5%
-33.26800
*. The mean difference is significant at the 0.05 level.
Persen Lintasan
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Suspensi Loperamid 0,52 mg/kg BB
5
45.5240
EEDS dosis 400 mg/kg BB
5
55.8420
55.8420
EEDS dosis 200 mg/kg BB
5
56.3720
56.3720
EEDS dosis 100 mg/kg BB
5
EEDS dosis 50 mg/kg BB
5
Suspensi Na-CMC 0,5%
5
66.4340
86.1840
89.1100
Sig.
.275
.298
.991
99
Universitas Sumatera Utara
59
Universitas Sumatera Utara
Lampiran 2. Surat rekomendasi persetujuan etik penelitian
60
Universitas Sumatera Utara
Lampiran 3. Gambar tanaman srikaya (Annona squamosa L.)
61
Universitas Sumatera Utara
Lampiran 4. Gambar daun segar dan serbuk simplisia daun srikaya
Gambar daun srikaya segar
Serbuk simplisia daun srikaya
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Lampiran 5. Bagan kerja penelitian
1.
Pemeriksaan karakteristik dan skrining fitokimia serta pembuatan Ekstrak
Etanol Daun Srikaya (EEDS)
Daun srikaya
Dikumpulkan, dicuci,
ditiriskan dan ditimbang berat
basahnya
Dikeringkan di lemari
pengering
Simplisia daun srikaya
Ditimbang berat kering
simplisia
Dihaluskan
Serbuk simplisia
Diperkolasi
menggunakan
etanol 96%
Karakterisasi
meliputi
- Makroskopik
- Mikroskopik
- Kadar air
- Kadar sari larut
dalam air
- Kadar sari larut
dalam etanol
- Kadar abu total
- Kadar abu tidak
larut asam
Skrining fitokimia
meliputi:
- Flavonoid
- Alkaloid
- Saponin
- Tanin
- Glikosida
- Steroid/
terpenoid
Perkolat
Di-rotary evaporator
Ekstrak etanol
daun srikaya
(EEDS)
63
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Lampiran 5. (Lanjutan)
2. Pengujian aktivitas antidiare EEDS pada mencit jantan yang diinduksi oleh
minyak jarak
30 ekor mencit jantan
Dibagi secara acak menjadi 6 kelompok
Ditimbang, dicatat berat badannya dan diberi
penandaan pada ekornya
Diberi perlakuan setiap kelompok : suspensi NaCMC 0,5% dosis 1% BB; suspensi loperamid dosis
0,52 mg/kg BB; EEDS dosis 50, 100, 200, dan 400
mg/kg BB secara oral
Diberi minyak jarak 0,5 ml tiap mencit secara oral
setelah 1 jam pemberian perlakuan
Diamati mencit selama 5 jam selang waktu 30
menit
Onset diare
(menit)
Frekuensi diare
(kali)
Konsistensi dan
berat feses
(mg)
Durasi diare
(menit)
64
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Lampiran 5. (Lanjutan)
3. Pengujian aktivitas antimotilitas EEDS dengan metode transit intestinal
30 ekor mencit jantan
Dibagi secara acak menjadi 6 kelompok
Ditimbang, dicatat berat badannya dan diberi
penandaan pada ekornya
Diberi perlakuan setiap kelompok : suspensi NaCMC 0,5% dosis 1% BB; suspensi loperamid
dosis 0,52 mg/kg BB; EEDS dosis 50, 100, 200,
dan 400 mg/kg BB secara oral
Diberi minyak jarak 0,5 ml tiap mencit secara oral
setelah 1 jam pemberian perlakuan
Diberi tinta cina sebanyak 0,1 ml/10 g secara oral
setelah 1 jam pemberian minyak jarak
Dibiarkan 1 jam, kemudian semua hewan
dikorbankan dengan cara dislokasi leher
Dilakukan pembedahan dan ususnya dikeluarkan
Diukur panjang usus yang dilalui marker tinta
cina
Persen lintasan marker (%)
65
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Lampiran 6. Gambar pemeriksaan mikroskopik serbuk simplisia daun srikaya
perbesaran 10 X 40
1
2
1
3 2
3
Keterangan:
1. Stomata tipe parasitik
2. Trikoma multiseluler
3. Berkas pengangkut tipe spiral
66
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Lampiran 7. Perhitungan hasil pemeriksaan karakteristik simplisia daun srikaya
1. Perhitungan penetapan kadar air
Kadar air simplisia =
No.
1
2
3
Berat Sampel (g)
5,0578
5,0412
5,0502
x 100%
Volume awal (ml)
1,9
1,9
1,9
a. Kadar air 1
=
x 100% = 9,88%
b. Kadar air 2
=
x 100% = 9,91%
c. Kadar air 3
=
x 100% = 9,90%
Volume akhir (ml)
2,4
2,4
2,4
Rata-rata kadar air =
= 9,89%
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Lampiran 7. (Lanjutan)
2. Perhitungan penetapan kadar sari larut air
Kadar sari larut air =
No.
1.
2.
3.
a.
100%
Berat sampel (g)
5,0067
5,0046
5,0041
Kadar sari larut air 1
Berat sari (g)
0,3258
0,3043
0,2930
=
100%
=
100%
=
100%
= 32,53%
b.
Kadar sari larut air 2
= 30,40%
c.
Kadar sari larut air 3
= 29,27%
Rata-rata kadar sari larut air
= 30,73%
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Lampiran 7. (Lanjutan)
3.
Perhitungan penetapan kadar sari larut etanol
Kadar sari larut etanol =
No.
1.
2.
3.
100%
Berat sampel (g)
5,0023
5,0024
5,0024
Berat sari (g)
0,1640
0,1741
0,1712
a. Kadar sari larut etanol 1 =
100%
= 16,39%
100%
b. Kadar sari larut etanol 2 =
= 17,40%
c. Kadar sari larut etanol 3 =
100%
= 17,11%
Rata-rata kadar sari larut etanol =
= 16,96%
69
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Lampiran 7. (Lanjutan)
4.
Perhitungan penetapan kadar abu total
Kadar abu total
No.
1
2
3
=
100%
Berat Sampel (g)
2,0249
2,0098
2,0068
a. Kadar abu total 1 =
Berat abu (g)
0,0682
0,0653
0,0707
100%
= 3,36%
100%
b. Kadar abu total 2 =
= 3,24%
c. Kadar abu total 3 =
100%
= 3,52%
Rata- rata kadar abu total
=
= 3,37%
70
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Lampiran 7. (Lanjutan)
5. Perhitungan kadar abu tidak larut dalam asam
Kadar abu tidak larut dalam asam
No.
1
2
3
=
Berat Sampel (g)
2,0249
2,0098
2,0068
a. Kadar abu tidak larut asam 1
100%
Berat abu (g)
0,0095
0,0070
0,0187
100%
=
= 0,46%
b. Kadar abu tidak larut asam 2
=
100%
= 0,34%
c. Kadar air tidak larut asam 3
=
100%
= 0,93%
Rata- rata kadar air tidak larut asam =
= 0,57%
71
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Lampiran 8. Gambar alat dan bahan yang diperlukan
Spuit dan oral sonde
Minyak jarak
72
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Lampiran 8. (Lanjutan)
Peralatan bedah hewan
Tinta cina
73
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Lampiran 9. Gambar pengamatan hewan
Pengamatan hewan setelah diinduksi minyak jarak
74
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Lampiran 10. Gambar hewan sebelum dan sesudah dibedah
Posisi hewan sebelum dibedah
Posisi hewan setelah dibedah
75
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Lampiran 11. Gambar usus halus yang dilintasi marker tinta cina
55 cm
50 cm
Lintasan marker pada kelompok kontrol negatif
13,5 cm
41 cm
Lintasan marker pada kelompok kontrol positif
48 cm
46 cm
42 cm
Lintasan marker pada kelompok EEDS dosis 50 mg/kg BB
30 cm
Lintasan marker pada kelompok EEDS dosis 100 mg/kg BB
76
Universitas Sumatera Utara
Lampiran 11. (Lanjutan)
59 cm
30 cm
Lintasan marker pada kelompok EEDS dosis 200 mg/kg BB
46,5 cm
18,5 cm
Lintasan marker pada kelompok EEDS dosis 400 mg/kg BB
77
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Lampiran 12. Contoh perhitungan dosis
Tabel konversi dosis antara jenis hewan dengan manusia (Darmono, 2011).
Tabel volume maksimal larutan sediaan uji yang diberikan pada hewan
Volume maksimal (ml)
Jenis hewan uji
i.v
i.m
i.p
s.c
Mencit
0,5
0,05
1,0
0,5-1,0
(20-30)
p.o
1,0
Tikus (100 g)
1,0
0,1
2,5
2,5
5,0
Hamster (50 g)
-
0,1
1-2
2,5
2,5
Marmut (250 g)
-
0,25
2-5
5,0
10,0
Merpati (300 g)
2,0
0,5
2,0
2,0
10,0
Kelinci (2,5 kg)
5-10
0,5
10-20
5-10
20,0
Kucing
(3 kg)
5-10
1,0
10-20
5-10
50,0
Anjing
(5 kg)
10-20
5,0
20-50
10,0
100,0
(Harmita dan Radji, 2008)
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Lampiran 12. (Lanjutan)
1. Perhitungan dosis suspensi Na-CMC 0,5% dosis 1% BB
- Na-CMC 0,5% =
= 5 mg/ml
- Dosis 1% BB diberikan pada mencit 25 g
-
× 25 g = 0,25 ml
- Volume yang diberikan pada mencit = 0,25 ml
2. Perhitungan dosis suspensi loperamid HCl
®
- Tiap tablet Lodia mengandung loperamid HCl 2 mg
- Dosis loperamid HCl untuk manusia adalah 2 tablet = 4 mg loperamid HCl
dan dosis maksimal 16 mg/hari.
- Dosis untuk mencit 20 g adalah:
( 4 mg – 16 mg) x 0,0026 = 0,0104 mg – 0,0416 mg
Dosis yang digunakan adalah 0,0104 mg/20 g = 0,52 mg/kg BB
- Menurut FI Edisi III, penetapan kadar tablet memerlukan 20 tablet, maka
®
diambil 20 tablet Lodia kemudian digerus dan ditimbang berat totalnya =
3160 mg
®
- Berat bahan aktif loperamid HCl dalam 20 tablet Lodia
adalah 2 mg/tab x
20 tab = 40 mg.
®
- Maka serbuk Lodia yang digunakan adalah
x 3160 mg = 41,08 mg ≈ 41 mg
- Cara pembuatan suspensi loperamid HCl
®
Ditimbang serbuk Lodia
sebanyak 41 mg digerus kemudian dilarutkan
dengan Na-CMC 0,5% hingga 10 ml (konsentrasi = 0,0052%)
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Universitas Sumatera Utara
Lampiran 12. (Lanjutan)
- Volume suspensi loperamid yang diberikan (misal berat badan = 25 g)
× 25 g ×
= 0,25 ml
3. Perhitungan dosis suspensi EEDS
- Suspensi EEDS dibuat dengan 4 variasi konsentrasi yaitu 0,5%, 1%, 2%,
dan 4%. EEDS ditimbang 50 mg, 100 mg, 200 mg dan 400 mg, masingmasing digerus didalam lumpang, ditambahkan suspensi Na-CMC 0,5%
sedikit demi sedikit dan dicukupkan sampai 10 ml.
- Volume suspensi EEDS 2% yang diberikan (misal berat badan = 23 g)
dengan dosis 200 mg/kg BB adalah
-
× 23 g ×
= 0,23 ml
80
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Lampiran 13. Data onset diare
Hewan kePerlakuan
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 50 mg/kg BB
EEDS dosis 100 mg/kg BB
EEDS dosis 200 mg/kg BB
EEDS dosis 400 mg/kg BB
1
2
3
4
5
80
88
94
75
83
RataRata
(menit)
84
124
138
147
131
156
139,2
90
109
113
136
82
103
127
142
97
98
110
129
105
107
118
125
94
114
124
117
93,6
106,2
118,4
129,5
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Lampiran 14. Data frekuensi diare
Perlakuan
Suspensi
Na-CMC
0,5%
Suspensi
loperamid
0,52 mg/kg
BB
EEDS
dosis 50
mg/kg BB
EEDS
dosis 100
mg/kg BB
EEDS
dosis 200
mg/kg BB
EEDS
dosis 400
mg/kg BB
1
0
0
0
0
0
2
0
0
0
0
0
3
1
1
2
1
1
Pengamatan ke4
5
6
7
3
2
1
2
1
1
2
2
2
1
1
1
1
3
2
3
2
2
1
2
8
1
1
1
2
1
9
1
1
1
2
1
10
1
1
1
1
1
0
0
0
0
1
2
2
0
0
0
Jumlah
(kali)
12
10
10
15
11
58
5
2
0
0
0
0
2
2
0
2
0
0
6
3
4
5
Jumlah
1
2
3
4
5
Jumlah
1
2
3
4
5
Jumlah
0
0
0
0
0
0
0
0
0
0
0
0
1
1
0
0
1
1
0
2
1
3
0
0
1
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
0
0
0
1
3
1
1
2
2
2
3
3
2
3
0
3
2
1
0
1
0
0
2
2
1
1
2
1
2
1
0
2
2
0
0
1
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
2
2
1
1
2
0
1
3
2
2
2
3
0
2
1
2
1
2
1
2
2
0
0
1
2
1
2
1
1
0
0
0
0
0
5
4
3
23
11
9
9
10
11
50
10
9
9
7
8
43
1
2
3
4
5
Jumlah
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
1
2
0
2
2
2
2
2
1
1
2
0
1
1
1
1
2
3
0
0
1
0
1
1
2
0
2
2
0
0
0
0
0
0
0
0
0
1
1
2
2
0
0
6
7
7
8
9
37
6
2
0
0
0
0
1
2
2
2
2
0
8
3
4
5
Jumlah
0
0
0
0
0
0
0
0
0
0
0
1
1
2
0
2
2
3
1
0
2
0
2
2
1
1
0
0
0
0
5
7
8
34
Mencit
ke1
2
3
4
5
Jumlah
1
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Lampiran 15. Data konsistensi dan berat feses
Perlakuan
Suspensi Na-CMC
0,5%
Suspensi loperamid
dosis 0,52 mg/kg BB
EEDS dosis 50 mg/kg
BB
EEDS dosis 100
mg/kg BB
EEDS dosis 200
mg/kg BB
EEDS dosis 400
mg/kg BB
Hewan ke
Padat
0,41
0,39
0,52
0,24
0,55
2,11
0,16
0,09
0,10
0,23
0,18
0,76
0,35
0,31
0,53
0,27
0,42
1,88
0,31
0,28
0,26
0,47
0,12
1,45
0,34
0,14
0,28
0,45
0,10
1,31
0,17
0,11
0,20
0,25
0,31
1,04
1
2
3
4
5
Jumlah
1
2
3
4
5
Jumlah
1
2
3
4
5
Jumlah
1
2
3
4
5
Jumlah
1
2
3
4
5
Jumlah
1
2
3
4
5
Jumlah
Berat feses (gram)
Berair
Lembek
0,23
1,29
0,62
1,64
0,43
1,37
0,51
1,55
0,50
1,32
2,29
7,17
0,11
0,68
0,13
0,46
0,23
0,75
0,21
0,47
0,19
0,54
0,87
2,90
0,46
1,05
0,31
0,90
0,42
1,51
0,25
1,46
0,37
1,39
1,81
6,31
0,28
0,58
0,25
0,77
0,14
0,80
0,29
1,13
0,36
1,26
1,32
4,54
0,13
0,98
0,32
0,75
0,17
0,99
0,22
0,53
0,29
0,57
1,13
3,82
0,39
0,88
0,17
0,65
0,24
0,89
0,21
0,43
0,10
0,47
1,11
3,32
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Lampiran 16. Data durasi diare
Hewan kePerlakuan
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 50 mg/kg BB
EEDS dosis 100 mg/kg BB
EEDS dosis 200 mg/kg BB
EEDS dosis 400 mg/kg BB
1
2
3
4
5
193
199
187
219
192
RataRata
(menit)
198
78
83
100
82
94
87,4
164
144
128
91
169
145
141
103
181
152
127
119
155
155
126
128
179
151
135
112
169,6
149,4
131,4
110,6
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Lampiran 17. Data persentase lintas marker
Perlakuan
Suspensi Na-CMC 0,5%
Suspensi Loperamid
dosis 0,52 mg/kg BB
EEDS dosis 50 mg/kg
BB
EEDS dosis 100 mg/kg
BB
EEDS dosis 200 mg/kg
BB
EEDS dosis 400 mg/kg
BB
Hewan
ke
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
Panjang
usus
dilintasi
marker
(cm)
43
37,5
50
43
56
20
22,5
35
13,5
21
42
43
41
50
47,5
30
33
37,5
28,5
34
30
22
30
30
25
30
18,5
34
37,5
28
Panjang
usus
% Lintasan
seluruhnya
(%)
(cm)
46
42,5
55
54
60
41,5
42,5
61
41
58
48
47
46
58
62
46
48
52
46
53
44
48
47
59
47
50,5
46,5
60
60
46
93,47
88,23
90,90
79,62
93,33
48,19
52,94
57,37
32,92
36,20
87,50
91,48
89,13
86,20
76,61
65,21
68,75
72,11
61,95
64,15
68,18
45,83
63,82
50,84
53,19
59,40
39,78
56,67
62,50
60,86
85
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Lampiran 18. Analisis statistik onset diare
Tests of Normality
a
Kolmogorov-Smirnov
Kelompok
Statistic
Waktu Awal Suspensi Na-CMC 0,5%
Diare
Suspensi Loperamid
0,52 mg/kg BB
df
.154
Shapiro-Wilk
Sig.
5
Statistic
df
Sig.
.200
*
.990
5
.981
.982
5
.947
.141
5
.200
*
EEDS dosis 50 mg/kg
BB
.145
5
.200
*
.997
5
.997
EEDS dosis 100 mg/kg
BB
.153
5
.200
*
.994
5
.993
EEDS dosis 200 mg/kg
BB
.183
5
.200
*
.949
5
.729
EEDS dosis 400 mg/kg
BB
.139
5
.200
*
.991
5
.983
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
Waktu Awal Diare
95% Confidence
Interval for Mean
N
Suspensi Na-CMC
0,5%
Suspensi Loperamid
0,52 mg/kg BB
EEDS dosis 50
mg/kg BB
EEDS dosis 100
mg/kg BB
EEDS dosis 200
mg/kg BB
EEDS dosis 400
mg/kg BB
Total
Mean
Std.
Deviation
Std.
Error
Lower
Bound
5
84.00
7.314
3.271
5
139.20
12.677
5
93.60
5
Upper
Bound Minimum Maximum
74.92
93.08
75
94
5.669
123.46 154.94
124
156
8.503
3.803
83.04 104.16
82
105
106.20
6.058
2.709
98.68 113.72
98
114
5
118.40
7.162
3.203
109.51 127.29
110
127
5
129.80
9.680
4.329
117.78 141.82
117
142
30
111.87
21.287
3.887
103.92 119.82
75
156
ANOVA
Waktu Awal Diare
Sum of
Squares
df
Mean Square
Between Groups
Within Groups
11268.667
1872.800
5
24
Total
13141.467
29
2253.733
78.033
F
28.882
Sig.
.000
86
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Lampiran 18. (Lanjutan)
Multiple Comparisons
Waktu Awal Diare
Tukey HSD
95% Confidence Interval
(I) Kelompok
Suspensi NaCMC 0,5%
(J) Kelompok
Mean Difference
(I-J)
Std. Error
Suspensi Loperamid 0,52
mg/kg BB
-55.200
EEDS dosis 50 mg/kg BB
Lower
Bound
Sig.
Upper Bound
*
5.587
.000
-72.47
-37.93
-9.600
5.587
.534
-26.87
7.67
EEDS dosis 100 mg/kg BB
-22.200*
5.587
.007
-39.47
-4.93
EEDS dosis 200 mg/kg BB
-34.400*
5.587
.000
-51.67
-17.13
EEDS dosis 400 mg/kg BB
-45.800*
5.587
.000
-63.07
-28.53
Suspensi
Suspensi Na-CMC 0,5%
Loperamid
EEDS dosis 50 mg/kg BB
0,52 mg/kg BB
EEDS dosis 100 mg/kg BB
55.200
*
5.587
.000
37.93
72.47
45.600*
5.587
.000
28.33
62.87
33.000*
5.587
.000
15.73
50.27
EEDS dosis 200 mg/kg BB
20.800*
5.587
.012
3.53
38.07
EEDS dosis 400 mg/kg BB
9.400
5.587
.556
-7.87
26.67
9.600
5.587
.534
-7.67
26.87
-45.600*
5.587
.000
-62.87
-28.33
EEDS dosis 100 mg/kg BB
-12.600
5.587
.251
-29.87
4.67
EEDS dosis 200 mg/kg BB
-24.800*
5.587
.002
-42.07
-7.53
EEDS dosis 400 mg/kg BB
-36.200*
5.587
.000
-53.47
-18.93
22.200*
5.587
.007
4.93
39.47
-33.000*
5.587
.000
-50.27
-15.73
EEDS dosis 50 mg/kg BB
12.600
5.587
.251
-4.67
29.87
EEDS dosis 200 mg/kg BB
-12.200
5.587
.281
-29.47
5.07
EEDS dosis 400 mg/kg BB
*
EEDS dosis 50 Suspensi Na-CMC 0,5%
mg/kg BB
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis
Suspensi Na-CMC 0,5%
100 mg/kg BB
Suspensi Loperamid 0,52
mg/kg BB
5.587
.004
-40.87
-6.33
EEDS dosis
Suspensi Na-CMC 0,5%
200 mg/kg BB Suspensi Loperamid 0,52
mg/kg BB
-23.600
34.400*
5.587
.000
17.13
51.67
-20.800*
5.587
.012
-38.07
-3.53
EEDS dosis 50 mg/kg BB
24.800*
5.587
.002
7.53
42.07
EEDS dosis 100 mg/kg BB
12.200
5.587
.281
-5.07
29.47
EEDS dosis 400 mg/kg BB
-11.400
5.587
.350
-28.67
5.87
*
EEDS dosis
Suspensi Na-CMC 0,5%
400 mg/kg BB
Suspensi Loperamid 0,52
mg/kg BB
45.800
5.587
.000
28.53
63.07
-9.400
5.587
.556
-26.67
7.87
EEDS dosis 50 mg/kg BB
36.200*
5.587
.000
18.93
53.47
EEDS dosis 100 mg/kg BB
23.600*
5.587
.004
6.33
40.87
EEDS dosis 200 mg/kg BB
11.400
5.587
.350
-5.87
28.67
Waktu Awal Diare
Tukey HSD
a
Subset for alpha = 0.05
Kelompok
N
1
2
Suspensi Na-CMC 0,5%
5
84.00
EEDS dosis 50 mg/kg BB
5
93.60
EEDS dosis 100 mg/kg BB
5
EEDS dosis 200 mg/kg BB
5
EEDS dosis 400 mg/kg BB
5
Suspensi Loperamid 0,52 mg/kg BB
5
3
4
5
93.60
106.20
106.20
118.40
118.40
129.80
129.80
139.20
Sig.
.534
.251
.281
.350
.556
87
Universitas Sumatera Utara
Lampiran 19. Analisis statistik frekuensi diare
Tests of Normality
a
Kolmogorov-Smirnov
Kelompok
Frekuensi
Diare
Statistic
Suspensi Na-CMC 0,5%
df
.224
Shapiro-Wilk
Sig.
5
Statistic
df
Sig.
.200
*
.842
5
.171
.961
5
.814
Suspensi Loperamid
0,52 mg/kg BB
.237
5
.200
*
EEDS dosis 50 mg/kg
BB
.241
5
.200
*
.821
5
.119
EEDS dosis 100 mg/kg
BB
.237
5
.200
*
.961
5
.814
EEDS dosis 200 mg/kg
BB
.237
5
.200
*
.961
5
.814
EEDS dosis 400 mg/kg
BB
.221
5
.200
*
.902
5
.421
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
Frekuensi Diare
95% Confidence
Interval for
Mean
Std.
Mean Deviation
N
Suspensi Na-CMC
0,5%
Suspensi Loperamid
0,52 mg/kg BB
EEDS dosis 50 mg/kg
BB
EEDS dosis 100 mg/kg
BB
EEDS dosis 200 mg/kg
BB
EEDS dosis 400 mg/kg
BB
Total
Std.
Error
Lower Upper
Bound Bound Minimum Maximum
5
11.60
2.074
.927
9.03
14.17
10
15
5
4.60
1.140
.510
3.18
6.02
3
6
5
10.00
1.000
.447
8.76
11.24
9
11
5
8.60
1.140
.510
7.18
10.02
7
10
5
7.40
1.140
.510
5.98
8.82
6
9
5
6.80
1.304
.583
5.18
8.42
5
8
30
8.17
2.601
.475
7.20
9.14
3
15
ANOVA
Frekuensi Diare
Sum of
Squares
Df
Mean Square
Between Groups
Within Groups
152.567
43.600
5
24
Total
196.167
29
30.513
1.817
F
16.796
Sig.
.000
88
Universitas Sumatera Utara
Lampiran 19. (Lanjutan)
Multiple Comparisons
Frekuensi Diare
Tukey HSD
(I) Kelompok
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 50 mg/kg BB
Mean
Difference (I-J) Std. Error
(J) Kelompok
95% Confidence Interval
Sig.
Lower Bound
Upper Bound
*
7.000
.852
.000
4.36
9.64
EEDS dosis 50 mg/kg BB
1.600
.852
.439
-1.04
4.24
EEDS dosis 100 mg/kg BB
3.000
*
.852
.019
.36
5.64
*
6.84
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 200 mg/kg BB
4.200
.852
.001
1.56
EEDS dosis 400 mg/kg BB
4.800
*
.852
.000
2.16
7.44
Suspensi Na-CMC 0,5%
-7.000*
.852
.000
-9.64
-4.36
EEDS dosis 50 mg/kg BB
-5.400*
.852
.000
-8.04
-2.76
EEDS dosis 100 mg/kg BB
-4.000*
.852
.001
-6.64
-1.36
EEDS dosis 200 mg/kg BB
-2.800*
.852
.033
-5.44
-.16
EEDS dosis 400 mg/kg BB
-2.200
.852
.141
-4.84
.44
Suspensi Na-CMC 0,5%
-1.600
.852
.439
-4.24
1.04
Suspensi Loperamid 0,52
mg/kg BB
5.400
*
.852
.000
2.76
8.04
EEDS dosis 100 mg/kg BB
1.400
.852
.580
-1.24
4.04
EEDS dosis 200 mg/kg BB
2.600
.852
.055
-.04
5.24
EEDS dosis 400 mg/kg BB
3.200
*
.852
.011
.56
5.84
*
.852
.019
-5.64
-.36
EEDS dosis 100 mg/kg BB Suspensi Na-CMC 0,5%
-3.000
Suspensi Loperamid 0,52
mg/kg BB
4.000
*
.852
.001
1.36
6.64
EEDS dosis 50 mg/kg BB
-1.400
.852
.580
-4.04
1.24
EEDS dosis 200 mg/kg BB
1.200
.852
.722
-1.44
3.84
EEDS dosis 400 mg/kg BB
1.800
.852
.315
-.84
4.44
*
.852
.001
-6.84
-1.56
5.44
EEDS dosis 200 mg/kg BB Suspensi Na-CMC 0,5%
-4.200
Suspensi Loperamid 0,52
mg/kg BB
2.800
*
.852
.033
.16
EEDS dosis 50 mg/kg BB
-2.600
.852
.055
-5.24
.04
EEDS dosis 100 mg/kg BB
-1.200
.852
.722
-3.84
1.44
EEDS dosis 400 mg/kg BB
.600
.852
.980
-2.04
3.24
-4.800*
.852
.000
-7.44
-2.16
Suspensi Loperamid 0,52
mg/kg BB
2.200
.852
.141
-.44
4.84
EEDS dosis 50 mg/kg BB
-3.200
*
.852
.011
-5.84
-.56
EEDS dosis 100 mg/kg BB
-1.800
.852
.315
-4.44
.84
EEDS dosis 200 mg/kg BB
-.600
.852
.980
-3.24
2.04
EEDS dosis 400 mg/kg BB Suspensi Na-CMC 0,5%
*. The mean difference is significant at the 0.05 level.
Frekuensi Diare
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Suspensi Loperamid 0,52 mg/kg BB
5
4.60
EEDS dosis 400 mg/kg BB
5
6.80
EEDS dosis 200 mg/kg BB
5
7.40
EEDS dosis 100 mg/kg BB
5
8.60
EEDS dosis 50 mg/kg BB
5
Suspensi Na-CMC 0,5%
5
4
6.80
7.40
8.60
10.00
10.00
11.60
Sig.
.141
.315
.055
.439
89
Universitas Sumatera Utara
Lampiran 20. Analisis statistik konsistensi dan berat feses padat
Tests of Normality
a
Kolmogorov-Smirnov
Kelompok
Berat
Feses
Padat
Statistic
df
Shapiro-Wilk
Sig.
Statistic
df
Sig.
Suspensi Na-CMC
0,5%
.197
5
.200
*
.935
5
.631
Suspensi Loperamid
0,52 mg/kg BB
.215
5
.200
*
.937
5
.642
EEDS dosis 50 mg/kg
BB
.200
5
.200
*
.948
5
.723
EEDS dosis 100
mg/kg BB
.230
5
.200
*
.955
5
.774
EEDS dosis 200
mg/kg BB
.202
5
.200
*
.952
5
.752
EEDS dosis 400
mg/kg BB
.142
5
.200
*
.996
5
.995
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
Berat Feses Padat
95% Confidence
Interval for Mean
Std.
Mean Deviation
N
Suspensi Na-CMC
0,5%
Suspensi Loperamid
0,52 mg/kg BB
EEDS dosis 50 mg/kg
BB
EEDS dosis 100 mg/kg
BB
EEDS dosis 200 mg/kg
BB
EEDS dosis 400 mg/kg
BB
Total
Std.
Error
Lower
Bound
Upper
Bound Minimum Maximum
5
.4220
.12276 .05490
.2696
.5744
.24
.55
5
.1520
.05805 .02596
.0799
.2241
.09
.23
5
.3760
.10237 .04578
.2489
.5031
.27
.53
5
.2880
.12518 .05598
.1326
.4434
.12
.47
5
.2620
.14394 .06437
.0833
.4407
.10
.45
5
.2080
.07629 .03412
.1133
.3027
.11
.31
30
.2847
.13653 .02493
.2337
.3356
.09
.55
ANOVA
Berat Feses Padat
Sum of
Squares
df
Mean Square
Between Groups
Within Groups
.256
.285
5
24
Total
.541
29
.051
.012
F
Sig.
4.319
.006
90
Universitas Sumatera Utara
Lampiran 20. (Lanjutan)
Multiple Comparisons
Berat Feses Padat
Tukey HSD
Mean
Difference (I-J) Std. Error
95% Confidence Interval
(I) Kelompok
(J) Kelompok
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
.27000
*
.06886
.007
.0571
.4829
EEDS dosis 50 mg/kg BB
.04600
.06886
.984
-.1669
.2589
EEDS dosis 100 mg/kg BB
.13400
.06886
.400
-.0789
.3469
EEDS dosis 200 mg/kg BB
.16000
.06886
.224
-.0529
.3729
EEDS dosis 400 mg/kg BB
.21400
*
.06886
.048
.0011
.4269
*
.06886
.007
-.4829
-.0571
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 50 mg/kg BB
Sig.
Lower Bound
Upper Bound
Suspensi Na-CMC 0,5%
-.27000
EEDS dosis 50 mg/kg BB
-.22400
*
.06886
.035
-.4369
-.0111
EEDS dosis 100 mg/kg BB
-.13600
.06886
.385
-.3489
.0769
EEDS dosis 200 mg/kg BB
-.11000
.06886
.608
-.3229
.1029
EEDS dosis 400 mg/kg BB
-.05600
.06886
.962
-.2689
.1569
Suspensi Na-CMC 0,5%
-.04600
.06886
.984
-.2589
.1669
Suspensi Loperamid 0,52
mg/kg BB
.22400
*
.06886
.035
.0111
.4369
EEDS dosis 100 mg/kg BB
.08800
.06886
.794
-.1249
.3009
EEDS dosis 200 mg/kg BB
.11400
.06886
.572
-.0989
.3269
EEDS dosis 400 mg/kg BB
.16800
.06886
.183
-.0449
.3809
-.13400
.06886
.400
-.3469
.0789
Suspensi Loperamid 0,52
mg/kg BB
.13600
.06886
.385
-.0769
.3489
EEDS dosis 50 mg/kg BB
-.08800
.06886
.794
-.3009
.1249
EEDS dosis 200 mg/kg BB
.02600
.06886
.999
-.1869
.2389
EEDS dosis 400 mg/kg BB
.08000
.06886
.850
-.1329
.2929
-.16000
.06886
.224
-.3729
.0529
Suspensi Loperamid 0,52
mg/kg BB
.11000
.06886
.608
-.1029
.3229
EEDS dosis 50 mg/kg BB
-.11400
.06886
.572
-.3269
.0989
EEDS dosis 100 mg/kg BB
-.02600
.06886
.999
-.2389
.1869
EEDS dosis 400 mg/kg BB
.05400
.06886
.968
-.1589
.2669
-.21400*
.06886
.048
-.4269
-.0011
Suspensi Loperamid 0,52
mg/kg BB
.05600
.06886
.962
-.1569
.2689
EEDS dosis 50 mg/kg BB
-.16800
.06886
.183
-.3809
.0449
EEDS dosis 100 mg/kg BB
-.08000
.06886
.850
-.2929
.1329
EEDS dosis 200 mg/kg BB
-.05400
.06886
.968
-.2669
.1589
EEDS dosis 100 mg/kg BB Suspensi Na-CMC 0,5%
EEDS dosis 200 mg/kg BB Suspensi Na-CMC 0,5%
EEDS dosis 400 mg/kg BB Suspensi Na-CMC 0,5%
*. The mean difference is significant at the 0.05 level.
Berat Feses Padat
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Suspensi Loperamid 0,52 mg/kg BB
5
.1520
EEDS dosis 400 mg/kg BB
5
.2080
.2080
EEDS dosis 200 mg/kg BB
5
.2620
.2620
.2620
EEDS dosis 100 mg/kg BB
5
.2880
.2880
.2880
EEDS dosis 50 mg/kg BB
5
.3760
.3760
Suspensi Na-CMC 0,5%
5
.4220
Sig.
.385
.183
.224
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
91
Universitas Sumatera Utara
Lampiran 21. Analisis statistik konsistensi dan berat feses berair
Tests of Normality
a
Kolmogorov-Smirnov
Kelompok
Berat Feses
Berair
Statistic
df
Shapiro-Wilk
Sig.
Statistic
df
Sig.
Suspensi Na-CMC
0,5%
.223
5
.200
*
Suspensi Loperamid
0,52 mg/kg BB
.221
5
.200
*
.915
5
.501
EEDS dosis 50 mg/kg
BB
.155
5
.200
*
.976
5
.911
EEDS dosis 100
mg/kg BB
.231
5
.200
*
.945
5
.699
EEDS dosis 200
mg/kg BB
.189
5
.200
*
.952
5
.750
EEDS dosis 400
mg/kg BB
.234
5
.200
*
.950
5
.735
.929
5
.591
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
Berat Feses Berair
95%
Confidence
Interval for
Mean
Std.
Mean Deviation
N
Suspensi Na-CMC 0,5%
Suspensi Loperamid
0,52 mg/kg BB
EEDS dosis 50 mg/kg
BB
EEDS dosis 100 mg/kg
BB
EEDS dosis 200 mg/kg
BB
EEDS dosis 400 mg/kg
BB
Total
Std.
Error
Lower Upper
Bound Bound Minimum Maximum
5
5
.4580
.1740
.14446
.05177
.06461
.02315
.2786
.1097
.6374
.2383
.23
.11
.62
.23
5
.3620
.08408
.03760
.2576
.4664
.25
.46
5
.2640
.08019
.03586
.1644
.3636
.14
.36
5
.2260
.07956
.03558
.1272
.3248
.13
.32
5
.2220
.10756
.04810
.0884
.3556
.10
.39
30
.2843
.13132
.02398
.2353
.3334
.10
.62
ANOVA
Berat Feses Berair
Sum of Squares
df
Mean Square
Between Groups
Within Groups
.280
.220
5
24
Total
.500
29
.056
.009
F
Sig.
6.122
.001
92
Universitas Sumatera Utara
Lampiran 21. (Lanjutan)
Multiple Comparisons
Berat Feses Berair
Tukey HSD
(I) Kelompok
Suspensi Na-CMC 0,5%
Mean
Difference (I-J) Std. Error
(J) Kelompok
EEDS dosis 50 mg/kg BB
Lower Bound
Upper Bound
*
.28400
.06053
.001
.0969
.4711
EEDS dosis 50 mg/kg BB
.09600
.06053
.615
-.0911
.2831
EEDS dosis 100 mg/kg BB
.19400
*
.06053
.039
.0069
.3811
EEDS dosis 200 mg/kg BB
.23200
*
.06053
.009
.0449
.4191
*
.06053
.008
.0489
.4231
*
.06053
.001
-.4711
-.0969
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 400 mg/kg BB
Suspensi Loperamid 0,52
mg/kg BB
95% Confidence Interval
Sig.
.23600
Suspensi Na-CMC 0,5%
-.28400
EEDS dosis 50 mg/kg BB
-.18800
*
.06053
.048
-.3751
-.0009
EEDS dosis 100 mg/kg BB
-.09000
.06053
.675
-.2771
.0971
EEDS dosis 200 mg/kg BB
-.05200
.06053
.953
-.2391
.1351
EEDS dosis 400 mg/kg BB
-.04800
.06053
.966
-.2351
.1391
Suspensi Na-CMC 0,5%
-.09600
.06053
.615
-.2831
.0911
Suspensi Loperamid 0,52
mg/kg BB
.18800
*
.06053
.048
.0009
.3751
EEDS dosis 100 mg/kg BB
.09800
.06053
.595
-.0891
.2851
EEDS dosis 200 mg/kg BB
.13600
.06053
.254
-.0511
.3231
EEDS dosis 400 mg/kg BB
.14000
.06053
.228
-.0471
.3271
-.19400
*
.06053
.039
-.3811
-.0069
Suspensi Loperamid 0,52
mg/kg BB
.09000
.06053
.675
-.0971
.2771
EEDS dosis 50 mg/kg BB
-.09800
.06053
.595
-.2851
.0891
EEDS dosis 200 mg/kg BB
.03800
.06053
.988
-.1491
.2251
EEDS dosis 400 mg/kg BB
.04200
.06053
.981
-.1451
.2291
-.23200
*
.06053
.009
-.4191
-.0449
Suspensi Loperamid 0,52
mg/kg BB
.05200
.06053
.953
-.1351
.2391
EEDS dosis 50 mg/kg BB
-.13600
.06053
.254
-.3231
.0511
EEDS dosis 100 mg/kg BB
-.03800
.06053
.988
-.2251
.1491
EEDS dosis 400 mg/kg BB
.00400
.06053
1.000
-.1831
.1911
-.23600
*
.06053
.008
-.4231
-.0489
Suspensi Loperamid 0,52
mg/kg BB
.04800
.06053
.966
-.1391
.2351
EEDS dosis 50 mg/kg BB
-.14000
.06053
.228
-.3271
.0471
EEDS dosis 100 mg/kg BB
-.04200
.06053
.981
-.2291
.1451
EEDS dosis 200 mg/kg BB
-.00400
.06053
1.000
-.1911
.1831
EEDS dosis 100 mg/kg BB Suspensi Na-CMC 0,5%
EEDS dosis 200 mg/kg BB Suspensi Na-CMC 0,5%
EEDS dosis 400 mg/kg BB Suspensi Na-CMC 0,5%
*. The mean difference is significant at the 0.05 level.
Berat Feses Berair
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Suspensi Loperamid 0,52 mg/kg BB
5
.1740
EEDS dosis 400 mg/kg BB
5
.2220
.2220
EEDS dosis 200 mg/kg BB
5
.2260
.2260
EEDS dosis 100 mg/kg BB
5
.2640
.2640
EEDS dosis 50 mg/kg BB
5
Suspensi Na-CMC 0,5%
5
.3620
.3620
.4580
Sig.
.675
.228
.615
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
93
Universitas Sumatera Utara
Lampiran 22. Analisis statistik konsistensi dan berat feses lembek
Tests of Normality
a
Kolmogorov-Smirnov
Kelompok
Berat
Feses
Lembek
Statistic
Suspensi Na-CMC 0,5%
df
Shapiro-Wilk
Sig.
.201
5
Statistic
df
Sig.
.200
*
.951
5
.747
.884
5
.330
Suspensi Loperamid
0,52 mg/kg BB
.221
5
.200
*
EEDS dosis 50 mg/kg
BB
.282
5
.200
*
.868
5
.259
EEDS dosis 100 mg/kg
BB
.251
5
.200
*
.932
5
.611
EEDS dosis 200 mg/kg
BB
.237
5
.200
*
.908
5
.455
EEDS dosis 400 mg/kg
BB
.239
5
.200
*
.861
5
.230
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
Berat Feses Lembek
95%
Confidence
Interval for
Mean
Std.
Mean Deviation
N
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 50 mg/kg BB
EEDS dosis 100 mg/kg
BB
EEDS dosis 200 mg/kg
BB
EEDS dosis 400 mg/kg
BB
Total
Std.
Error
Lower Upper
Bound Bound Minimum Maximum
5 1.3800
5 .5800
.23969
.12942
.10719 1.0824 1.6776
.05788 .4193 .7407
1.02
.46
1.64
.75
5 1.2620
5 .9080
.27068
.27905
.12105 .9259 1.5981
.12480 .5615 1.2545
.90
.58
1.51
1.26
5
.7840
.19970
.08931 .5360 1.0320
.53
.99
5
.6640
.21813
.09755 .3932 .9348
.43
.89
30
.9297
.36625
.06687 .7929 1.0664
.43
1.64
ANOVA
Berat Feses Lembek
Sum of
Squares
Mean
Square
df
Between Groups
Within Groups
2.639
1.251
5
24
Total
3.890
29
F
.528
.052
10.124
Sig.
.000
94
Universitas Sumatera Utara
Lampiran 22. (Lanjutan)
Multiple Comparisons
Berat Feses Lembek
Tukey HSD
(I) Kelompok
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 50 mg/kg BB
95% Confidence Interval
Mean
Difference (I-J) Std. Error
(J) Kelompok
Sig.
Lower Bound
Upper Bound
*
.80000
.14441
.000
.3535
1.2465
EEDS dosis 50 mg/kg BB
.11800
.14441
.961
-.3285
.5645
EEDS dosis 100 mg/kg BB
.47200
*
.14441
.034
.0255
.9185
EEDS dosis 200 mg/kg BB
.59600
*
.14441
.005
.1495
1.0425
EEDS dosis 400 mg/kg BB
.71600*
.14441
.001
.2695
1.1625
Suspensi Na-CMC 0,5%
-.80000*
.14441
.000
-1.2465
-.3535
EEDS dosis 50 mg/kg BB
-.68200*
.14441
.001
-1.1285
-.2355
EEDS dosis 100 mg/kg BB
-.32800
.14441
.244
-.7745
.1185
EEDS dosis 200 mg/kg BB
-.20400
.14441
.719
-.6505
.2425
EEDS dosis 400 mg/kg BB
-.08400
.14441
.991
-.5305
.3625
Suspensi Na-CMC 0,5%
-.11800
.14441
.961
-.5645
.3285
Suspensi Loperamid 0,52
mg/kg BB
.68200
*
.14441
.001
.2355
1.1285
EEDS dosis 100 mg/kg BB
.35400
.14441
.179
-.0925
.8005
EEDS dosis 200 mg/kg BB
.47800
*
.14441
.031
.0315
.9245
EEDS dosis 400 mg/kg BB
.59800
*
.14441
.004
.1515
1.0445
-.47200
*
.14441
.034
-.9185
-.0255
Suspensi Loperamid 0,52
mg/kg BB
.32800
.14441
.244
-.1185
.7745
EEDS dosis 50 mg/kg BB
-.35400
.14441
.179
-.8005
.0925
EEDS dosis 200 mg/kg BB
.12400
.14441
.953
-.3225
.5705
EEDS dosis 400 mg/kg BB
.24400
.14441
.551
-.2025
.6905
-.59600
*
.14441
.005
-1.0425
-.1495
Suspensi Loperamid 0,52
mg/kg BB
.20400
.14441
.719
-.2425
.6505
EEDS dosis 50 mg/kg BB
-.47800*
.14441
.031
-.9245
-.0315
EEDS dosis 100 mg/kg BB
-.12400
.14441
.953
-.5705
.3225
EEDS dosis 400 mg/kg BB
.12000
.14441
.959
-.3265
.5665
-.71600
*
.14441
.001
-1.1625
-.2695
Suspensi Loperamid 0,52
mg/kg BB
.08400
.14441
.991
-.3625
.5305
EEDS dosis 50 mg/kg BB
-.59800
*
.14441
.004
-1.0445
-.1515
EEDS dosis 100 mg/kg BB
-.24400
.14441
.551
-.6905
.2025
EEDS dosis 200 mg/kg BB
-.12000
.14441
.959
-.5665
.3265
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 100 mg/kg BB Suspensi Na-CMC 0,5%
EEDS dosis 200 mg/kg BB Suspensi Na-CMC 0,5%
EEDS dosis 400 mg/kg BB Suspensi Na-CMC 0,5%
*. The mean difference is significant at the 0.05 level.
Berat Feses Lembek
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
Suspensi Loperamid 0,52 mg/kg BB
5
.5800
EEDS dosis 400 mg/kg BB
5
.6640
EEDS dosis 200 mg/kg BB
5
.7840
EEDS dosis 100 mg/kg BB
5
.9080
EEDS dosis 50 mg/kg BB
5
Suspensi Na-CMC 0,5%
5
Sig.
3
.9080
1.2620
1.2620
1.3800
.244
.179
.961
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
95
Universitas Sumatera Utara
Lampiran 23. Analisis statistik durasi diare
Tests of Normality
a
Kolmogorov-Smirnov
Kelompok
Lama
Diare
Statistic
Suspensi Na-CMC 0,5%
df
Shapiro-Wilk
Sig.
.268
5
Statistic
df
Sig.
.200
*
.845
5
.178
.905
5
.437
Suspensi Loperamid 0,52
mg/kg BB
.284
5
.200
*
EEDS dosis 50 mg/kg BB
.209
5
.200
*
.943
5
.688
*
.908
5
.457
EEDS dosis 100 mg/kg BB
.233
5
.200
EEDS dosis 200 mg/kg BB
.302
5
.155
.857
5
.219
5
*
.991
5
.982
EEDS dosis 400 mg/kg BB
.139
.200
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
Lama Diare
95% Confidence
Interval for Mean
N
Suspensi Na-CMC
0,5%
Suspensi Loperamid
0,52 mg/kg BB
EEDS dosis 50 mg/kg
BB
EEDS dosis 100
mg/kg BB
EEDS dosis 200
mg/kg BB
EEDS dosis 400
mg/kg BB
Total
Mean
Std.
Deviation
5
198.00
12.490
5
87.40
9.209
5
169.60
5
Std.
Error
Lower
Bound
5.586 182.49
Upper
Bound Minimum Maximum
213.51
187
219
75.97
98.83
78
100
10.761
4.812 156.24
182.96
155
181
149.40
4.722
2.112 143.54
155.26
144
155
5
131.40
6.427
2.874 123.42
139.38
126
141
5
110.60
14.293
6.392
92.85
128.35
91
128
30
141.07
38.341
7.000 126.75
155.38
78
219
4.118
ANOVA
Lama Diare
Sum of Squares
df
Mean Square
Between Groups
Within Groups
40133.867
2498.000
5
24
Total
42631.867
29
8026.773
104.083
F
77.119
Sig.
.000
Lampiran 23. (Lanjutan)
96
Universitas Sumatera Utara
Multiple Comparisons
Lama Diare
Tukey HSD
(I) Kelompok
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
EEDS dosis 50 mg/kg BB
Mean
Difference (I-J) Std. Error
(J) Kelompok
Lower Bound
*
6.452
.000
*
Suspensi Loperamid 0,52
mg/kg BB
110.600
EEDS dosis 50 mg/kg BB
28.400
6.452
EEDS dosis 100 mg/kg BB
48.600
*
6.452
EEDS dosis 200 mg/kg BB
66.600*
EEDS dosis 400 mg/kg BB
Upper Bound
90.65
130.55
.002
8.45
48.35
.000
28.65
68.55
6.452
.000
46.65
86.55
*
6.452
.000
67.45
107.35
*
6.452
.000
-130.55
-90.65
*
6.452
.000
-102.15
-62.25
*
6.452
.000
-81.95
-42.05
*
6.452
.000
-63.95
-24.05
*
6.452
.016
-43.15
-3.25
*
6.452
.002
-48.35
-8.45
*
6.452
.000
62.25
102.15
*
6.452
.046
.25
40.15
*
6.452
.000
18.25
58.15
*
6.452
.000
39.05
78.95
*
6.452
.000
-68.55
-28.65
*
6.452
.000
42.05
81.95
87.400
Suspensi Na-CMC 0,5%
-110.600
EEDS dosis 50 mg/kg BB
-82.200
EEDS dosis 100 mg/kg BB
-62.000
EEDS dosis 200 mg/kg BB
-44.000
EEDS dosis 400 mg/kg BB
-23.200
Suspensi Na-CMC 0,5%
-28.400
Suspensi Loperamid 0,52
mg/kg BB
82.200
EEDS dosis 100 mg/kg BB
20.200
EEDS dosis 200 mg/kg BB
38.200
EEDS dosis 400 mg/kg BB
59.000
EEDS dosis 100 mg/kg BB Suspensi Na-CMC 0,5%
95% Confidence Interval
Sig.
-48.600
Suspensi Loperamid 0,52
mg/kg BB
62.000
EEDS dosis 50 mg/kg BB
-20.200
*
6.452
.046
-40.15
-.25
EEDS dosis 200 mg/kg BB
18.000
6.452
.094
-1.95
37.95
EEDS dosis 400 mg/kg BB
38.800
*
6.452
.000
18.85
58.75
*
6.452
.000
-86.55
-46.65
*
6.452
.000
24.05
63.95
-18.25
EEDS dosis 200 mg/kg BB Suspensi Na-CMC 0,5%
-66.600
Suspensi Loperamid 0,52
mg/kg BB
44.000
EEDS dosis 50 mg/kg BB
-38.200
*
6.452
.000
-58.15
EEDS dosis 100 mg/kg BB
-18.000
6.452
.094
-37.95
1.95
EEDS dosis 400 mg/kg BB
20.800
*
6.452
.038
.85
40.75
EEDS dosis 400 mg/kg BB Suspensi Na-CMC 0,5%
*
6.452
.000
-107.35
-67.45
*
6.452
.016
3.25
43.15
*
6.452
.000
-78.95
-39.05
*
6.452
.000
-58.75
-18.85
*
6.452
.038
-40.75
-.85
-87.400
Suspensi Loperamid 0,52
mg/kg BB
23.200
EEDS dosis 50 mg/kg BB
-59.000
EEDS dosis 100 mg/kg BB
-38.800
EEDS dosis 200 mg/kg BB
-20.800
*. The mean difference is significant at the 0.05 level.
Lama Diare
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
4
Suspensi Loperamid 0,52 mg/kg BB
5
EEDS dosis 400 mg/kg BB
5
EEDS dosis 200 mg/kg BB
5
131.40
EEDS dosis 100 mg/kg BB
5
149.40
EEDS dosis 50 mg/kg BB
5
Suspensi Na-CMC 0,5%
5
Sig.
5
87.40
110.60
169.60
198.00
1.000
1.000
.094
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
97
Universitas Sumatera Utara
Lampiran 24. Analisis statistik persentase lintas marker
Tests of Normality
a
Kolmogorov-Smirnov
Kelompok
Persen
Lintasan
Statistic
df
Shapiro-Wilk
Sig.
Statistic
df
Sig.
Suspensi Na-CMC
0,5%
.239
5
.200
*
Suspensi Loperamid
0,52 mg/kg BB
.211
5
.200
*
.919
5
.521
EEDS dosis 50 mg/kg
BB
.301
5
.157
.862
5
.235
EEDS dosis 100 mg/kg
BB
.220
5
.200
*
.960
5
.808
EEDS dosis 200 mg/kg
BB
.234
5
.200
*
.933
5
.617
EEDS dosis 400 mg/kg
BB
.336
5
.068
.762
5
.038
.832
5
.145
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
Persen Lintasan
95% Confidence
Interval for Mean
N
Suspensi Na-CMC
0,5%
Suspensi Loperamid
0,52 mg/kg BB
EEDS dosis 50 mg/kg
BB
EEDS dosis 100
mg/kg BB
EEDS dosis 200
mg/kg BB
EEDS dosis 400
mg/kg BB
Total
Mean
Std.
Deviation
Std.
Error
Lower
Bound
Upper
Bound Minimum Maximum
5 89.1100
5.71963 2.55790 82.0081 96.2119
79.62
93.47
5 45.5240
10.58572 4.73408 32.3801 58.6679
32.92
57.37
5 86.1840
5.70351 2.55069 79.1022 93.2658
76.61
91.48
5 66.4340
4.01254 1.79446 61.4518 71.4162
61.95
72.11
5 56.3720
9.31079 4.16391 44.8111 67.9329
45.83
68.18
5 55.8420
9.23095 4.12821 44.3803 67.3037
39.78
62.50
30 66.5777
17.84534 3.25810 59.9141 73.2412
32.92
93.47
ANOVA
Persen Lintasan
Sum of Squares
df
Mean Square
Between Groups
Within Groups
7774.010
1461.214
5
24
Total
9235.225
29
1554.802
60.884
F
25.537
Sig.
.000
98
Universitas Sumatera Utara
Lampiran 24. (Lanjutan)
Multiple Comparisons
Persen Lintasan
Tukey HSD
Mean
Difference (I-J) Std. Error
(I) Kelompok
(J) Kelompok
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
Lower Bound
Upper Bound
*
4.93493
.000
28.3275
58.8445
2.92600
4.93493
.991
-12.3325
18.1845
*
4.93493
.001
7.4175
37.9345
EEDS dosis 200 mg/kg BB
*
32.73800
4.93493
.000
17.4795
47.9965
EEDS dosis 400 mg/kg BB
33.26800*
4.93493
.000
18.0095
48.5265
Suspensi Na-CMC 0,5%
-43.58600*
4.93493
.000
-58.8445
-28.3275
EEDS dosis 50 mg/kg BB
-40.66000*
4.93493
.000
-55.9185
-25.4015
EEDS dosis 100 mg/kg BB
-20.91000
*
4.93493
.003
-36.1685
-5.6515
EEDS dosis 200 mg/kg BB
-10.84800
4.93493
.275
-26.1065
4.4105
EEDS dosis 400 mg/kg BB
-10.31800
4.93493
.325
-25.5765
4.9405
-2.92600
4.93493
.991
-18.1845
12.3325
*
4.93493
.000
25.4015
55.9185
*
4.93493
.006
4.4915
35.0085
*
4.93493
.000
14.5535
45.0705
*
4.93493
.000
15.0835
45.6005
*
4.93493
.001
-37.9345
-7.4175
36.1685
EEDS dosis 100 mg/kg BB
EEDS dosis 50 mg/kg BB
Sig.
43.58600
EEDS dosis 50 mg/kg BB
Suspensi Loperamid 0,52
mg/kg BB
95% Confidence Interval
22.67600
Suspensi Na-CMC 0,5%
Suspensi Loperamid 0,52
mg/kg BB
40.66000
EEDS dosis 100 mg/kg BB
19.75000
EEDS dosis 200 mg/kg BB
29.81200
EEDS dosis 400 mg/kg BB
30.34200
EEDS dosis 100 mg/kg BB Suspensi Na-CMC 0,5%
-22.67600
Suspensi Loperamid 0,52
mg/kg BB
20.91000
*
4.93493
.003
5.6515
EEDS dosis 50 mg/kg BB
-19.75000*
4.93493
.006
-35.0085
-4.4915
EEDS dosis 200 mg/kg BB
10.06200
4.93493
.351
-5.1965
25.3205
10.59200
4.93493
.298
-4.6665
25.8505
*
4.93493
.000
-47.9965
-17.4795
Suspensi Loperamid 0,52
mg/kg BB
10.84800
4.93493
.275
-4.4105
26.1065
EEDS dosis 50 mg/kg BB
-29.81200*
4.93493
.000
-45.0705
-14.5535
EEDS dosis 100 mg/kg BB
-10.06200
4.93493
.351
-25.3205
5.1965
EEDS dosis 400 mg/kg BB
.53000
4.93493
1.000
-14.7285
15.7885
*
4.93493
.000
-48.5265
-18.0095
Suspensi Loperamid 0,52
mg/kg BB
10.31800
4.93493
.325
-4.9405
25.5765
EEDS dosis 50 mg/kg BB
-30.34200
*
4.93493
.000
-45.6005
-15.0835
EEDS dosis 100 mg/kg BB
-10.59200
4.93493
.298
-25.8505
4.6665
EEDS dosis 200 mg/kg BB
-.53000
4.93493
1.000
-15.7885
14.7285
EEDS dosis 400 mg/kg BB
EEDS dosis 200 mg/kg BB Suspensi Na-CMC 0,5%
-32.73800
EEDS dosis 400 mg/kg BB Suspensi Na-CMC 0,5%
-33.26800
*. The mean difference is significant at the 0.05 level.
Persen Lintasan
Tukey HSDa
Subset for alpha = 0.05
Kelompok
N
1
2
3
Suspensi Loperamid 0,52 mg/kg BB
5
45.5240
EEDS dosis 400 mg/kg BB
5
55.8420
55.8420
EEDS dosis 200 mg/kg BB
5
56.3720
56.3720
EEDS dosis 100 mg/kg BB
5
EEDS dosis 50 mg/kg BB
5
Suspensi Na-CMC 0,5%
5
66.4340
86.1840
89.1100
Sig.
.275
.298
.991
99
Universitas Sumatera Utara