Establishment of micropropagation in dwarf napiergrass (Pennisetum purpureum Schumach) and estimation of somaclonal variation using flowcytometry analysis - repository civitas UGM
'$$sd,##*$JSCS E',::ffi*'JJJ,$:fi:
Japan ch na Korea
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of the .4th Japan-China-Korea Grassland Conference
A-fii. Japan
*'l$tApril2012
rd
etmr
l&rm
ftture challenges of grasslands and grassland agriculture in the East Asia
rte imernational, national and regional issues and challenges in grassland systems in the East Asia
pmr&ution and utilization in cropland-based systems
ma menagement of grassland-based systems
of,genetic resources and seed technology
b
tre Japanese Society of Grassland Science in collaboration with the Chinese Grassland Society and
Saiery- of Grassland and Forage Science
Crumittee
E*'u
H!
Cmto (Mie University, Japan)
Ilffiada (Obihiro University of Agriculture and Veterinary Medicine, Japan)
B{F Hou tl-anzhou University, China)
hf ffag llnner Mongolia Agricultural University, China)
h I-q China Agricultural University, China)
Lqf Rmg Lee (Irlational Institute of Animal Science, Korea)
]ftSfir r\ational Institute of Animal Science, Korea)
lFry Ta Jeon (Konkuk University, Korea)
fus Sashiki (NARO Institute of Livestock and Grassland Science, Japan)
lbi*i Sugi1-ama (Hirosaki University, Japan)
&Bfti Tamaki CNARO Institute of Livestock and Grassland Science, Japan)
(
Commiftee
h
\a1ota (Gifu University)
h*e Har*-amura (Hiroshima University)
&'
Ihndo (Mie University)
Er l[alsui (Mie University)
tn L-,ozumi (NARO TohokuAgricultural Research Center)
E*t Daido INARO Institute of Livestock and Grassland Science)
\{atoba (i.{ARo Institute of Livestock and Grassland Science)
$iro
frENakano (NARO Institute of Livestock and Grassland science)
Eo Sato (fiARo Institute of Livestock and Grassland science)
r;!* saaxara (NARO Institute of Livestock and Grassland science)
Elyimhi Ichinohe (Shimane University)
Dr ALdi tlrniversity of Miyazaki)
hftfro
Ei.ri
Encfti
ffie
Hirata (University of Miyazaki)
Tmaka flJniversity of Miyazaki)
Horiguchi (Yamagata University)
l-asuda (Yamanashi Institute of Environmental
S
ciences)
Published by the 4th JCK Grassland conference organizing committee
Website : https ://j ckgc .brc.miy azaki_u. ac jp/index.html
Printed by the TAISEI pdnt Co., Ltd.
Sponsors
Japanese Society of Grassland Science (JSGS)
Frontier Science Resaerch Center, University of Miyazaki
Fujiwara Scientifrc Co., Ltd.
Futaba Shiryou Co., Ltd.
HOKUREN Federation of Agricultural C ooperatives
HIGASHIYAMA-CHIIKIJYUKU, Kakegawa City, Shizuoka prefecture
Human Metabolome Technologies Inc.
Japan Nutrition Co., Ltd.
Meiji Seika Pharma Co., Ltd.
Surge Miyawaki Co., Ltd.
SNOWBRAND SEED Co., Ltd.
Tokachi Federation of Agricultural Cooperatives
YUKA SANGYO Co., Ltd.
Contents
Professor Masakazu Goto
Professor Jinfeng Yun
Professor Byong-tae Jeon
Floor map
Display booth at lobby
Crueral Information for Attendees
Registration and information desk
Check-in
Sadge distribution
llessage boards
$tteme
$
3 .................
tfreme 4 .................
""""':"""""'
.........203
.........267
Helcome Address
ffissor
Masakazu Goto
ot tre organizing committee of the fourlh Japan-China-Korea
Cmference hosted by the Japanese Society of Grassland Science in
ht si& the Chinese Society for Grassland Science and the Korean
C**{$Imd and Forage Science, I would like to extend my heartfelt
- aItr of 1ou attending the intemational scientific symposium on
and animal production.
n-as initially organized on the 50th anniversary
of
the
fuieil of Grassland Science in 2004, and is rounded back to Japan
Ib se grateful to all of you for the distinguished contributions for
rrir-ffies for this intemational conference which should let us move on to higher
stages
of scientific
grefteful and proud of all the participants for your scientific and kind cooperation. We have over 340
s ftis moment.
This is a big compensation for our experience that the conference scheduled in
ll'as regrettably cancelled due to the spread of foot and mouth disease. We also thank all invitees
q; contributors who have addressed to our main theme, 'Evolution and Future Challenges of
ryj Grassland Agriculture in East Asia'. We wish that the grassland scientists in Asian countries will
achange their valuable new findings and experiences, and also you will discuss our possibility of
acwibutions to the grassland science and agriculture all over the world.
I m ]tl1
o
i
rrc rmlXd like to express our sincere gratitude toward the numerous emails expressing your sympathies
ts for the victims of the big earthquake and tsunami that hit Northeast and Kanto Regions of
fne scidents at Fukushima Nuclear Power Plant.
aiiol-1our stay in Japan.
March 31,2012
Professor Dr. M. Goto
President, Japanese Society of Grassland Science
Welcome Address
Professor Jinfeng Yun
Ladies and gentlemen, and all our friends:
This is a beautiful season of the year in Japan with the cherry trees in
blooming and we are happy to be here and attend the fourlh
Japan-China-Korea grassland conference which successfully opens in Japan.
It is the beginning position ofthe second round ofjoint conferences.
ffi
On behalf of the Chinese Grassland Society, I offer my heartfelt
congratulations to the Japanese Society of Grassland Science and the
organization commifiee fot preparing this symposium and I anticipate a very
successful meeting.
Japan, China and Korea contain the major portion of the grassland area in East Asia. We are facing many salne
issues. In order to promote international cooperation in the field of grassland science, the first meeting "The
Hiroshima in 200-1'
Japan-Korea-China symposium on grassland agticulture and animal production" was held at
in 2006 ani
at
Lanzhou
.qtorum for the scientifiidiscussion *u, .r,purrrively succeeded to the second symposium
ani
experiences
exchange ideas, share
the third, symposium at Seoul in 2009. Ouiloint slnnposium promoted to
enhance friendshiP.
and future challenges c'
On the occasion when the meeting is hold again in Japan, with the aim of evolution
of the big earthquat'"
grasslands and grassland agdculture in Asia. o-rganizing committee overcame the diffrculties
for this conference' On behalf of a'and tsunami that hit Northeast and Kanto districis and completed many works
thanks to You'
the partrctpants otthe conterence,\wou\d\ike to give out sincere
trc\udirrg
mote sciendflc ateas
csrrtere\ce a\ss{os rssre pat\c\pa$s ftras. betote a1\d corets
"-:tua!
ptoductron and utt\izatron, eco\o[v an(management 9t c.tas$a1o-t111-^'::T-:^Y:1':f:::i:,*""Sli:r'":'"H=
pos'=
presentatioll,
of
,o orr. ih.r. ur. r*Ioo* activitfes duting the meeting, consistrng
,i.*ing and discussions etc. I believe it will be a fruitful and successful meeting'
\\rs
tJ"a"H$l::il"il;h
are-faced for us' such as shortage of resource--With the global economic integration, many-common issues
th;
and many others' Therefore' in the future' I suggest
deterioration of ecological environment, food safety
i:
research
the
on
strengthen and improve the cooperation
member countries could increase gradually so as to
gruttf*a among the Asia counties and its influence on the world'
China next'
Finally, I hope you have a good time and see you in
March 31,201:
Yr
Professor Jinfeng
Honorary President of Chinese Grassland Sociel
Welcome Address
Professor Byong-tae Jeon
Eello, members!
Fmt of all, I welcome all of you and thank you for your participation and
sooperation for the 4th Japan-China-Korea Grassland Conference.
r=hen I was in Japan 30 years ago studying at Tohoku university, a Japanese
Gilrepreneur told me that I should do my best to increase bilateral
derstanding and friendship between students from Korea and Japan if I
hcome a professor in Korea. He also predicted that the world would become
rconflicting design of "zone versus zone"; European countries would unite
-o a group in order to compete against the United States. He added that Asian countries should closely cooperate
rilh each other to win competitions in the international arena.
r same
;
"The
t2004.
06 and
;es and
Io'ill
-eqsive
X
rges of
hquake
tf of all
forage
remember the entrepreneur's vision that the three countries of Korea, Japan and China of East Asia would
the leader of the world, while on the other hand, they could face tremendous losses in case of making
hme
competitions with each other.
confident this Japan{hina-Korea Grassland Conference will not only encourage private researches in
science but also play a great role to enhance friendship and cooperation befween the three countries.
, I hope this event will become a greatplace for international scientific exchanges among researchers.
;rsUna
my special thanks go to all the staff members of the Japanese Society of Grassland Science who have
" the efforts preparing for the 4th Conference.
all
YOU.
sources
, poster
March 31,2012
iources,
Byong-tae Jeon, PhD
President, The Korean Society of Grassland and Forage Science
Professor, Konkuk University, Department of Animal S cience
;est that
larch of
ll,2012
'eng Yun
I
Society
7Program
30 March
1
(Friday)
31 March
15:00-18:00
Lobby
Registration
18:00-20:00
Centrair
Organizing Committee meeting
08:30-10:00
Room
Centrair
Hall
Opening ceremony (including keynote address)
Chairperson: Masaaki Hanada (Obihiro University of Agriculture
(Saturday)
and Veterinary Medicine, JaPan)
Keynote address: Mamoru Nashiki (NARO Institute of Livestock
and Grassland Science, JaPan)
10:00-10:15
Lobby
Coffee Break
10:15-12:15
Centrair
Presentations (sub theme 1)
Chairperson. Masahiko Hirata (University of Miyazaki,
Hall
Speaker 1: Xianglin
Li
Japan)
(Chinese Academy of Agricultural Science,
China)
Speaker 2: Jong Duk Kim (Cheonan Yonam College' Korea)
Speaker 3. Vanthong Phengvichith Q"lational Agriculture and
Forestl
Research Institute, Lao PDR)
firne, Organizing Committee business meeting
12:1544:40
Lobby
t-uncfr
14:00-16:00
Centrair
Hall
Presentations (sub theme 2)
Chairperson: Yuying Shen (Lanzhou Universiry China)
Centrair
Motohiko Ishida
Japan techno fair
Speaker 1: Yixin Shen (Nanjing Agricultural Universiry China)
Speaker 2: Jong Geun Kim (Cheonan Yonam College, Korea)
So
16:00-17:00
Hall
e
aker.3 :
(Ishikawa Prefectur4!
Ijg['ersiry !p3g]-
Chairperson: Shuichi Sugiyama (Vice President of
Japanese
Society of Grassland Science)
Opening address: Masakazu Goto (President of Japanese Society of
Grassland Science)
Presentation 1. YUKA SANGYO Co., Ltd.
Presentation 2. SNOWBRAND SEED Co., Ltd'
Closing address: Masakazu Goto (President of Japanese Society of
Crassland Science)
April
3
18:30-20:30
Toyoko lnn
Partv room
Conference PartY
08:30-1 0:1 5
Room
R1_R3
Poster viewing (sub themes 1 and 2)
10:00-10:15
Lobby
Coffee Break
10:15-12'.15
Centrair
Hall
Presentations (sub theme 3)
C h a irp er s o n : Mikinori Tsuiki (Iwate University, Japan)
Speaker 1: Tiangang Liang (I-anzhou University, China)
ipeaker 2: Nariyasu Watanabe (NARO Hokkaido Agricultural
Sunday)
I
Research Center, JaPan)
Lunch Time
12:15-14:04
14:00-16:00
Centrair
Hall
16:00-17:45
Room
R1_R3
Centrair
Hall
17:45-1 B:00
4-5
2-3 April
(MondayTuesday)
Whole days
Presentations (sub theme 4)
Chairperson:Hyo Won Lee (Korea National Open Universiry Kod
Speoie, 1. Xinquan Zhang (Sichuan Agricultural University, Chirq
Speaker 2. T.I. Park Q"lational Institute of Crop Science' Korea)
Soeaker J: Rvo Akashi (Universitv of MiyazqE!-!p3'n)Poster viewing (sub themes 3 and 4)
Closlng ceremony Announcement for the next meeting
kserrtations
ffom
ffin
address: 31 March, Saturday, 8:30-10:00
of forage in Japan: Current state and future
.br"\-eslriki
perspective...
.............. l0
Q'{ARO Institute of Livestock and Grassland Science, Japan)
1
peerrtation 31 March, Saturday, 10:15-12:15
$ustainable management of grassland systems in China: issues and
fufut Li Onstitute
epnlcforage
challenges......... ........
l8
of Animal Science, Chinese Academy of Agricultural Science, China)
production system for animalproduction in
Korea.....
.........24
.fr llr* Eiw (Cheonan Yonam College, Korea), Chqn Ho Kwon (Kyung-Pook National Universie, Korea), Hyo
hl-e tKorea National Open University, Korea) and Chang-Hyun Kim (Hanlqtong National University)
Bassland system in Metnam: history, present condition and prospects.............. ...............30
$Sa Huu Yan (Hue University of Agriculture and Forestry, Vietnam)
)
Tlearrrrentstatus of forage research and development in Lao
*rE
PDR....
Phengvichith (|{ational Agriculture and Forestry Research Institute, Lao PDR)
1
..............34
April, Sunday, 08:30-10:15
2
31 March, Saturday, 14:00-16:00
Dl &searches on seasonal cultivation of alfalfa (Medicago sativa L) in southeastern
hsat!!"
China.
....
lt8
J
Huixin Jiang, Huabai Ha, Xiaoyan Mei (College of Animal Science and Tbchnologt, Nanjing Agricultural "\
fu:lrr Chtuu) and Qingsheng Cai (College of Life Sciences, Nanjing Agricultural (Jniversity, China)
fisategyfor the production of high-quality forage cultivated in paddy fields in Korea........ ..........124
.\fur
)iety
of
frn
(l'{ational Institute ofAnimal Science, Korea), Hyung Soo Park, Ki Chun Choi and Young Chul Lim
advances in the research on production and utilization of whole crop rice silage in Japan... 130
(Department of Bioproduction Science, Ishikawa Prefectural (Jniversity, Japan)
-Holslrida
1
iety
April, Sunday, 08:30-10:15
of
3
: 1 April, Sunday, 10:15-12:15
Bassland dynamics on Tibetan Plateau based on cscs and remote sensing.
.............204
futry Liang, Qisheng Feng, Hui Yu, Xiaodong Huang, Huilong Lin (Lanzhou [Jniversity, China), ShuzhouAn
f,n
:-+grczltural universie, china) and Jizhou Ren (Lanzhou []niversity, China)
Ilutubn of an accelerometer for animal behavior research in a grazing system...... ..................210
fiFlr Wbtansbe, Seiichi Sakanoue (NARO Hokkaido Agricultural Research Center, Japan) and Kensuke
hrr
rHiroshima University, Japan)
4
1
ry
Korea)
ry, China)
rrea)
April, Sunday, 14:00-16:00
H,rati
0.05) between
regenerated plants from multiple-shoot clumps (4.7
+ 0.33 pg iC-]j *o contror prants by FCM analysis.
Statistical
analysis of morphological chatacteristics ttowr.ro
rlgrin"ii, oirzuent in morphological traits between regenerants
and control plants (Table
4).
In
conclusion' we have.established multiple-shoot clumps
formation system in dwarf napiergrass (pennisetum
purpureum Schum)' The high number of iegenerants
could produce from shoot-apices of shoot tillers and
the
regenerants have same morphological characteristics
and DNA
,vith the control;h* Accordingly, this system
is stable and suitable
"oot"ni
#I#*r"ffi:Jf
H9g""
2.4-D
for production of nursery plants on o*u.i"ufi"rgrass
in the future.
hormone concentration on the formation or,n,rit pf,-shoot
clumps derived from shoot apices in tiller
BAp
shoot
02600u0
0.01
2
0.1
2
-": different
letters
apices
60
69
"r*rJd.**,o,
"rrr_rs
9u
17"
29
0
i6
followirg
t4
.05) on
experiment of multiple-shoot clump s formation.
Table 2. Effect of hormone and CuSOo on p.oli
2.4-DBAPEinoculatedproliferatedproliferatedrate1%oor
0.1
0.1
-:
25$6u9.52+
l"tt".r
iz'
fo
m
I1l,|,f,,:;rffilrl*:m:""oncentration
tiller of dwarf napiergrass
Io*or"
NAA BAp
-
o 2
:9' I
-:
different
34.e2
ll
on plant regeneration from riuttipre-shoot
,rvvl wruurPD
clumpr'o"rir.o
uErrvEu uum
from snool
shoot aplces
apices
clumps
(%)
$
e3
rupz.zjb
G23)^b
s3 (84.1)b
Shoot formation
33
63
folln
l"fi".r
f napiergrass
ffi
clumps
z
different
formation
0
;i^
;;'
158"
Table 4' Morphological characteristics of regenerants
from multi"ple-shoot crumps of dwarf napiergrass and r.
control
olants
Plant
PH (cm)
Regenerantro
128.8
Control"t
:
t28.6
(cm)
131.9
(cm)
7o.s
LBL
134.0 77.4
Non significants.
-zt
LBW (cm)
2.s
TN (cm)
Yield (gram)
23.6
735.4
2t.6
'731.6
References
Gondo T' Akashi R' Kawamurao (2004) Effect of
BAP and cupric sulfat_e on embryogenic callus formation
and plant
regeneration in bahiagrass (Paspalum n:otatum Fliigge).
Grassl icq 50: 55-5g gn rapai"se with English
summary).
rshigaki G' Gondo T' Ebina M, Suenaga
K, Akashi i (zotol Estimation of genome si ze in Brachiara
species. Grassl
Sci,56: 240142.
Murashige T, Skoog F (1962)
Plant, 15: 473497.
A revised medium for rapid growth and bioassay with tabacco
tissue culture. physiol
Japan ch na Korea
ffiwwKwKmffi $e ffi KeW ffiwwmxww
ffiWeffiffiKffiffieWwffi ffiW Wm,ffiffiwKmmMm
ffimM WtrffiffiffiMmmxM
ffiwffi&ffiffiffi&extrw ffim KKxffi ffimwK ffimffim
of the .4th Japan-China-Korea Grassland Conference
A-fii. Japan
*'l$tApril2012
rd
etmr
l&rm
ftture challenges of grasslands and grassland agriculture in the East Asia
rte imernational, national and regional issues and challenges in grassland systems in the East Asia
pmr&ution and utilization in cropland-based systems
ma menagement of grassland-based systems
of,genetic resources and seed technology
b
tre Japanese Society of Grassland Science in collaboration with the Chinese Grassland Society and
Saiery- of Grassland and Forage Science
Crumittee
E*'u
H!
Cmto (Mie University, Japan)
Ilffiada (Obihiro University of Agriculture and Veterinary Medicine, Japan)
B{F Hou tl-anzhou University, China)
hf ffag llnner Mongolia Agricultural University, China)
h I-q China Agricultural University, China)
Lqf Rmg Lee (Irlational Institute of Animal Science, Korea)
]ftSfir r\ational Institute of Animal Science, Korea)
lFry Ta Jeon (Konkuk University, Korea)
fus Sashiki (NARO Institute of Livestock and Grassland Science, Japan)
lbi*i Sugi1-ama (Hirosaki University, Japan)
&Bfti Tamaki CNARO Institute of Livestock and Grassland Science, Japan)
(
Commiftee
h
\a1ota (Gifu University)
h*e Har*-amura (Hiroshima University)
&'
Ihndo (Mie University)
Er l[alsui (Mie University)
tn L-,ozumi (NARO TohokuAgricultural Research Center)
E*t Daido INARO Institute of Livestock and Grassland Science)
\{atoba (i.{ARo Institute of Livestock and Grassland Science)
$iro
frENakano (NARO Institute of Livestock and Grassland science)
Eo Sato (fiARo Institute of Livestock and Grassland science)
r;!* saaxara (NARO Institute of Livestock and Grassland science)
Elyimhi Ichinohe (Shimane University)
Dr ALdi tlrniversity of Miyazaki)
hftfro
Ei.ri
Encfti
ffie
Hirata (University of Miyazaki)
Tmaka flJniversity of Miyazaki)
Horiguchi (Yamagata University)
l-asuda (Yamanashi Institute of Environmental
S
ciences)
Published by the 4th JCK Grassland conference organizing committee
Website : https ://j ckgc .brc.miy azaki_u. ac jp/index.html
Printed by the TAISEI pdnt Co., Ltd.
Sponsors
Japanese Society of Grassland Science (JSGS)
Frontier Science Resaerch Center, University of Miyazaki
Fujiwara Scientifrc Co., Ltd.
Futaba Shiryou Co., Ltd.
HOKUREN Federation of Agricultural C ooperatives
HIGASHIYAMA-CHIIKIJYUKU, Kakegawa City, Shizuoka prefecture
Human Metabolome Technologies Inc.
Japan Nutrition Co., Ltd.
Meiji Seika Pharma Co., Ltd.
Surge Miyawaki Co., Ltd.
SNOWBRAND SEED Co., Ltd.
Tokachi Federation of Agricultural Cooperatives
YUKA SANGYO Co., Ltd.
Contents
Professor Masakazu Goto
Professor Jinfeng Yun
Professor Byong-tae Jeon
Floor map
Display booth at lobby
Crueral Information for Attendees
Registration and information desk
Check-in
Sadge distribution
llessage boards
$tteme
$
3 .................
tfreme 4 .................
""""':"""""'
.........203
.........267
Helcome Address
ffissor
Masakazu Goto
ot tre organizing committee of the fourlh Japan-China-Korea
Cmference hosted by the Japanese Society of Grassland Science in
ht si& the Chinese Society for Grassland Science and the Korean
C**{$Imd and Forage Science, I would like to extend my heartfelt
- aItr of 1ou attending the intemational scientific symposium on
and animal production.
n-as initially organized on the 50th anniversary
of
the
fuieil of Grassland Science in 2004, and is rounded back to Japan
Ib se grateful to all of you for the distinguished contributions for
rrir-ffies for this intemational conference which should let us move on to higher
stages
of scientific
grefteful and proud of all the participants for your scientific and kind cooperation. We have over 340
s ftis moment.
This is a big compensation for our experience that the conference scheduled in
ll'as regrettably cancelled due to the spread of foot and mouth disease. We also thank all invitees
q; contributors who have addressed to our main theme, 'Evolution and Future Challenges of
ryj Grassland Agriculture in East Asia'. We wish that the grassland scientists in Asian countries will
achange their valuable new findings and experiences, and also you will discuss our possibility of
acwibutions to the grassland science and agriculture all over the world.
I m ]tl1
o
i
rrc rmlXd like to express our sincere gratitude toward the numerous emails expressing your sympathies
ts for the victims of the big earthquake and tsunami that hit Northeast and Kanto Regions of
fne scidents at Fukushima Nuclear Power Plant.
aiiol-1our stay in Japan.
March 31,2012
Professor Dr. M. Goto
President, Japanese Society of Grassland Science
Welcome Address
Professor Jinfeng Yun
Ladies and gentlemen, and all our friends:
This is a beautiful season of the year in Japan with the cherry trees in
blooming and we are happy to be here and attend the fourlh
Japan-China-Korea grassland conference which successfully opens in Japan.
It is the beginning position ofthe second round ofjoint conferences.
ffi
On behalf of the Chinese Grassland Society, I offer my heartfelt
congratulations to the Japanese Society of Grassland Science and the
organization commifiee fot preparing this symposium and I anticipate a very
successful meeting.
Japan, China and Korea contain the major portion of the grassland area in East Asia. We are facing many salne
issues. In order to promote international cooperation in the field of grassland science, the first meeting "The
Hiroshima in 200-1'
Japan-Korea-China symposium on grassland agticulture and animal production" was held at
in 2006 ani
at
Lanzhou
.qtorum for the scientifiidiscussion *u, .r,purrrively succeeded to the second symposium
ani
experiences
exchange ideas, share
the third, symposium at Seoul in 2009. Ouiloint slnnposium promoted to
enhance friendshiP.
and future challenges c'
On the occasion when the meeting is hold again in Japan, with the aim of evolution
of the big earthquat'"
grasslands and grassland agdculture in Asia. o-rganizing committee overcame the diffrculties
for this conference' On behalf of a'and tsunami that hit Northeast and Kanto districis and completed many works
thanks to You'
the partrctpants otthe conterence,\wou\d\ike to give out sincere
trc\udirrg
mote sciendflc ateas
csrrtere\ce a\ss{os rssre pat\c\pa$s ftras. betote a1\d corets
"-:tua!
ptoductron and utt\izatron, eco\o[v an(management 9t c.tas$a1o-t111-^'::T-:^Y:1':f:::i:,*""Sli:r'":'"H=
pos'=
presentatioll,
of
,o orr. ih.r. ur. r*Ioo* activitfes duting the meeting, consistrng
,i.*ing and discussions etc. I believe it will be a fruitful and successful meeting'
\\rs
tJ"a"H$l::il"il;h
are-faced for us' such as shortage of resource--With the global economic integration, many-common issues
th;
and many others' Therefore' in the future' I suggest
deterioration of ecological environment, food safety
i:
research
the
on
strengthen and improve the cooperation
member countries could increase gradually so as to
gruttf*a among the Asia counties and its influence on the world'
China next'
Finally, I hope you have a good time and see you in
March 31,201:
Yr
Professor Jinfeng
Honorary President of Chinese Grassland Sociel
Welcome Address
Professor Byong-tae Jeon
Eello, members!
Fmt of all, I welcome all of you and thank you for your participation and
sooperation for the 4th Japan-China-Korea Grassland Conference.
r=hen I was in Japan 30 years ago studying at Tohoku university, a Japanese
Gilrepreneur told me that I should do my best to increase bilateral
derstanding and friendship between students from Korea and Japan if I
hcome a professor in Korea. He also predicted that the world would become
rconflicting design of "zone versus zone"; European countries would unite
-o a group in order to compete against the United States. He added that Asian countries should closely cooperate
rilh each other to win competitions in the international arena.
r same
;
"The
t2004.
06 and
;es and
Io'ill
-eqsive
X
rges of
hquake
tf of all
forage
remember the entrepreneur's vision that the three countries of Korea, Japan and China of East Asia would
the leader of the world, while on the other hand, they could face tremendous losses in case of making
hme
competitions with each other.
confident this Japan{hina-Korea Grassland Conference will not only encourage private researches in
science but also play a great role to enhance friendship and cooperation befween the three countries.
, I hope this event will become a greatplace for international scientific exchanges among researchers.
;rsUna
my special thanks go to all the staff members of the Japanese Society of Grassland Science who have
" the efforts preparing for the 4th Conference.
all
YOU.
sources
, poster
March 31,2012
iources,
Byong-tae Jeon, PhD
President, The Korean Society of Grassland and Forage Science
Professor, Konkuk University, Department of Animal S cience
;est that
larch of
ll,2012
'eng Yun
I
Society
7Program
30 March
1
(Friday)
31 March
15:00-18:00
Lobby
Registration
18:00-20:00
Centrair
Organizing Committee meeting
08:30-10:00
Room
Centrair
Hall
Opening ceremony (including keynote address)
Chairperson: Masaaki Hanada (Obihiro University of Agriculture
(Saturday)
and Veterinary Medicine, JaPan)
Keynote address: Mamoru Nashiki (NARO Institute of Livestock
and Grassland Science, JaPan)
10:00-10:15
Lobby
Coffee Break
10:15-12:15
Centrair
Presentations (sub theme 1)
Chairperson. Masahiko Hirata (University of Miyazaki,
Hall
Speaker 1: Xianglin
Li
Japan)
(Chinese Academy of Agricultural Science,
China)
Speaker 2: Jong Duk Kim (Cheonan Yonam College' Korea)
Speaker 3. Vanthong Phengvichith Q"lational Agriculture and
Forestl
Research Institute, Lao PDR)
firne, Organizing Committee business meeting
12:1544:40
Lobby
t-uncfr
14:00-16:00
Centrair
Hall
Presentations (sub theme 2)
Chairperson: Yuying Shen (Lanzhou Universiry China)
Centrair
Motohiko Ishida
Japan techno fair
Speaker 1: Yixin Shen (Nanjing Agricultural Universiry China)
Speaker 2: Jong Geun Kim (Cheonan Yonam College, Korea)
So
16:00-17:00
Hall
e
aker.3 :
(Ishikawa Prefectur4!
Ijg['ersiry !p3g]-
Chairperson: Shuichi Sugiyama (Vice President of
Japanese
Society of Grassland Science)
Opening address: Masakazu Goto (President of Japanese Society of
Grassland Science)
Presentation 1. YUKA SANGYO Co., Ltd.
Presentation 2. SNOWBRAND SEED Co., Ltd'
Closing address: Masakazu Goto (President of Japanese Society of
Crassland Science)
April
3
18:30-20:30
Toyoko lnn
Partv room
Conference PartY
08:30-1 0:1 5
Room
R1_R3
Poster viewing (sub themes 1 and 2)
10:00-10:15
Lobby
Coffee Break
10:15-12'.15
Centrair
Hall
Presentations (sub theme 3)
C h a irp er s o n : Mikinori Tsuiki (Iwate University, Japan)
Speaker 1: Tiangang Liang (I-anzhou University, China)
ipeaker 2: Nariyasu Watanabe (NARO Hokkaido Agricultural
Sunday)
I
Research Center, JaPan)
Lunch Time
12:15-14:04
14:00-16:00
Centrair
Hall
16:00-17:45
Room
R1_R3
Centrair
Hall
17:45-1 B:00
4-5
2-3 April
(MondayTuesday)
Whole days
Presentations (sub theme 4)
Chairperson:Hyo Won Lee (Korea National Open Universiry Kod
Speoie, 1. Xinquan Zhang (Sichuan Agricultural University, Chirq
Speaker 2. T.I. Park Q"lational Institute of Crop Science' Korea)
Soeaker J: Rvo Akashi (Universitv of MiyazqE!-!p3'n)Poster viewing (sub themes 3 and 4)
Closlng ceremony Announcement for the next meeting
kserrtations
ffom
ffin
address: 31 March, Saturday, 8:30-10:00
of forage in Japan: Current state and future
.br"\-eslriki
perspective...
.............. l0
Q'{ARO Institute of Livestock and Grassland Science, Japan)
1
peerrtation 31 March, Saturday, 10:15-12:15
$ustainable management of grassland systems in China: issues and
fufut Li Onstitute
epnlcforage
challenges......... ........
l8
of Animal Science, Chinese Academy of Agricultural Science, China)
production system for animalproduction in
Korea.....
.........24
.fr llr* Eiw (Cheonan Yonam College, Korea), Chqn Ho Kwon (Kyung-Pook National Universie, Korea), Hyo
hl-e tKorea National Open University, Korea) and Chang-Hyun Kim (Hanlqtong National University)
Bassland system in Metnam: history, present condition and prospects.............. ...............30
$Sa Huu Yan (Hue University of Agriculture and Forestry, Vietnam)
)
Tlearrrrentstatus of forage research and development in Lao
*rE
PDR....
Phengvichith (|{ational Agriculture and Forestry Research Institute, Lao PDR)
1
..............34
April, Sunday, 08:30-10:15
2
31 March, Saturday, 14:00-16:00
Dl &searches on seasonal cultivation of alfalfa (Medicago sativa L) in southeastern
hsat!!"
China.
....
lt8
J
Huixin Jiang, Huabai Ha, Xiaoyan Mei (College of Animal Science and Tbchnologt, Nanjing Agricultural "\
fu:lrr Chtuu) and Qingsheng Cai (College of Life Sciences, Nanjing Agricultural (Jniversity, China)
fisategyfor the production of high-quality forage cultivated in paddy fields in Korea........ ..........124
.\fur
)iety
of
frn
(l'{ational Institute ofAnimal Science, Korea), Hyung Soo Park, Ki Chun Choi and Young Chul Lim
advances in the research on production and utilization of whole crop rice silage in Japan... 130
(Department of Bioproduction Science, Ishikawa Prefectural (Jniversity, Japan)
-Holslrida
1
iety
April, Sunday, 08:30-10:15
of
3
: 1 April, Sunday, 10:15-12:15
Bassland dynamics on Tibetan Plateau based on cscs and remote sensing.
.............204
futry Liang, Qisheng Feng, Hui Yu, Xiaodong Huang, Huilong Lin (Lanzhou [Jniversity, China), ShuzhouAn
f,n
:-+grczltural universie, china) and Jizhou Ren (Lanzhou []niversity, China)
Ilutubn of an accelerometer for animal behavior research in a grazing system...... ..................210
fiFlr Wbtansbe, Seiichi Sakanoue (NARO Hokkaido Agricultural Research Center, Japan) and Kensuke
hrr
rHiroshima University, Japan)
4
1
ry
Korea)
ry, China)
rrea)
April, Sunday, 14:00-16:00
H,rati
0.05) between
regenerated plants from multiple-shoot clumps (4.7
+ 0.33 pg iC-]j *o contror prants by FCM analysis.
Statistical
analysis of morphological chatacteristics ttowr.ro
rlgrin"ii, oirzuent in morphological traits between regenerants
and control plants (Table
4).
In
conclusion' we have.established multiple-shoot clumps
formation system in dwarf napiergrass (pennisetum
purpureum Schum)' The high number of iegenerants
could produce from shoot-apices of shoot tillers and
the
regenerants have same morphological characteristics
and DNA
,vith the control;h* Accordingly, this system
is stable and suitable
"oot"ni
#I#*r"ffi:Jf
H9g""
2.4-D
for production of nursery plants on o*u.i"ufi"rgrass
in the future.
hormone concentration on the formation or,n,rit pf,-shoot
clumps derived from shoot apices in tiller
BAp
shoot
02600u0
0.01
2
0.1
2
-": different
letters
apices
60
69
"r*rJd.**,o,
"rrr_rs
9u
17"
29
0
i6
followirg
t4
.05) on
experiment of multiple-shoot clump s formation.
Table 2. Effect of hormone and CuSOo on p.oli
2.4-DBAPEinoculatedproliferatedproliferatedrate1%oor
0.1
0.1
-:
25$6u9.52+
l"tt".r
iz'
fo
m
I1l,|,f,,:;rffilrl*:m:""oncentration
tiller of dwarf napiergrass
Io*or"
NAA BAp
-
o 2
:9' I
-:
different
34.e2
ll
on plant regeneration from riuttipre-shoot
,rvvl wruurPD
clumpr'o"rir.o
uErrvEu uum
from snool
shoot aplces
apices
clumps
(%)
$
e3
rupz.zjb
G23)^b
s3 (84.1)b
Shoot formation
33
63
folln
l"fi".r
f napiergrass
ffi
clumps
z
different
formation
0
;i^
;;'
158"
Table 4' Morphological characteristics of regenerants
from multi"ple-shoot crumps of dwarf napiergrass and r.
control
olants
Plant
PH (cm)
Regenerantro
128.8
Control"t
:
t28.6
(cm)
131.9
(cm)
7o.s
LBL
134.0 77.4
Non significants.
-zt
LBW (cm)
2.s
TN (cm)
Yield (gram)
23.6
735.4
2t.6
'731.6
References
Gondo T' Akashi R' Kawamurao (2004) Effect of
BAP and cupric sulfat_e on embryogenic callus formation
and plant
regeneration in bahiagrass (Paspalum n:otatum Fliigge).
Grassl icq 50: 55-5g gn rapai"se with English
summary).
rshigaki G' Gondo T' Ebina M, Suenaga
K, Akashi i (zotol Estimation of genome si ze in Brachiara
species. Grassl
Sci,56: 240142.
Murashige T, Skoog F (1962)
Plant, 15: 473497.
A revised medium for rapid growth and bioassay with tabacco
tissue culture. physiol