PENGARUH PENAMBAHAN Lactobacillus sp PADA PEMBUATAN SILASE PUCUK TEBU (Saccharum officinarum) TERHADAP POPULASI BAKTERI DAN PROTOZOA CAIRAN RUMEN DOMBA (In Vitro).
PENGARUH PENAMBAHAN Lactobacillus sp PADA PEMBUATAN
SILASE PUCUK TEBU (Saccharum officinarum) TERHADAP
POPULASI BAKTERI DAN PROTOZOA CAIRAN
RUMEN DOMBA (In Vitro)
Mochammad Pribadie Nugraha
ABSTRAK
Penelitian ini dilaksanakan di Laboratorium Nutrisi Ternak
Ruminansia, Balai Penelitian Ternak, Ciawi, Bogor pada bulan 1 Juni – 20
Juli 2013. Tujuan penelitian ini adalah mengetahui pengaruh penambahan
Lactobacillus sp dalam pembuatan silase pucuk tebu (Saccharum
officinarum) terhadap populasi bakteri dan protozoa cairan rumen domba
(in vitro). Penelitian ini dilaksanakan secara eksperimental menggunakan
Rancangan Acak Lengkap (RAL) dengan 3 perlakuan yaitu, P0 (pucuk tebu
tanpa fermentasi dan aditif), P1 (silase pucuk tebu dengan penambahan
molases 3%), P2 (silase pucuk tebu dengan penambahan molases 3% dan
Lactobacillus sp 0,5%). Setiap perlakuan diulang sebanyak 6 kali. Peubah
yang diamati adalah jumlah populasi bakteri dan protozoa cairan rumen
domba (In Vitro). Data dianalisa menggunakan analisis varian dan
dilanjutkan dengan Uji Contrast Orthogonal. Hasil penelitian menunjukkan
bahwa pemberian molases dan Lactobacillus sp memberikan pengaruh
nyata terhadap bakteri rumen, namun tidak berpengaruh nyata terhadap
protozoa rumen. Penambahan molases 3% pada silase pucuk tebu (P1)
menghasilkan jumlah populasi bakteri yang paling baik.
Kata Kunci: Pucuk Tebu, Silase, Bakteri, Protozoa, in vitro
iii
THE EFFECT OF Lactobacillus sp ADDITION AT SUGARCANE
TOPS ENSILAGE (Saccharum officinarum) ON TOTAL
BACTERIA AND PROTOZOA OF SHEEP
RUMEN LIQUID (IN VITRO)
Mochammad Pribadie Nugraha
ABSTRACT
The research was conducted at Laboratory of Ruminant Nutrition,
Balai Penelitian Ternak Ciawi, Bogor on 1 June – 20 July 2013. The
objective of research was to find out the effect of Lactobacillus sp addition
at sugarcane tops ensilage (Saccharum officinarum) on bacteria and
protozoa population of sheep rumen liquid (in vitro). This research used an
experimental method with a Completely Randomized Design (CRD). There
are 3 kind of treatments namely: P0 (sugarcane tops without fermentation
and additive), P1 (sugarcane tops silage added with molasses 3%), P2
(sugarcane tops silage added with molasses 3% and Lactobacillus sp 0,5%)
and each treatments was replicated 6 times. The observed parameters were
total bacteria and protozoa population of sheep rumen liquid (In Vitro). The
data were analyzed by variant analysis and followed by Contrast
Orthogonal Test. The result of research indicated that molasses and
Lactobacillus sp addition had significant effect on bacteria rumen, but had
no effect on protozoa rumen. The additions of molasses 3% on sugarcane
tops ensilage (P1) have the best result on total population of bacteria.
Key words: Sugarcane Tops, Silage, Bacteria, Protozoa, in vitro
iv
SILASE PUCUK TEBU (Saccharum officinarum) TERHADAP
POPULASI BAKTERI DAN PROTOZOA CAIRAN
RUMEN DOMBA (In Vitro)
Mochammad Pribadie Nugraha
ABSTRAK
Penelitian ini dilaksanakan di Laboratorium Nutrisi Ternak
Ruminansia, Balai Penelitian Ternak, Ciawi, Bogor pada bulan 1 Juni – 20
Juli 2013. Tujuan penelitian ini adalah mengetahui pengaruh penambahan
Lactobacillus sp dalam pembuatan silase pucuk tebu (Saccharum
officinarum) terhadap populasi bakteri dan protozoa cairan rumen domba
(in vitro). Penelitian ini dilaksanakan secara eksperimental menggunakan
Rancangan Acak Lengkap (RAL) dengan 3 perlakuan yaitu, P0 (pucuk tebu
tanpa fermentasi dan aditif), P1 (silase pucuk tebu dengan penambahan
molases 3%), P2 (silase pucuk tebu dengan penambahan molases 3% dan
Lactobacillus sp 0,5%). Setiap perlakuan diulang sebanyak 6 kali. Peubah
yang diamati adalah jumlah populasi bakteri dan protozoa cairan rumen
domba (In Vitro). Data dianalisa menggunakan analisis varian dan
dilanjutkan dengan Uji Contrast Orthogonal. Hasil penelitian menunjukkan
bahwa pemberian molases dan Lactobacillus sp memberikan pengaruh
nyata terhadap bakteri rumen, namun tidak berpengaruh nyata terhadap
protozoa rumen. Penambahan molases 3% pada silase pucuk tebu (P1)
menghasilkan jumlah populasi bakteri yang paling baik.
Kata Kunci: Pucuk Tebu, Silase, Bakteri, Protozoa, in vitro
iii
THE EFFECT OF Lactobacillus sp ADDITION AT SUGARCANE
TOPS ENSILAGE (Saccharum officinarum) ON TOTAL
BACTERIA AND PROTOZOA OF SHEEP
RUMEN LIQUID (IN VITRO)
Mochammad Pribadie Nugraha
ABSTRACT
The research was conducted at Laboratory of Ruminant Nutrition,
Balai Penelitian Ternak Ciawi, Bogor on 1 June – 20 July 2013. The
objective of research was to find out the effect of Lactobacillus sp addition
at sugarcane tops ensilage (Saccharum officinarum) on bacteria and
protozoa population of sheep rumen liquid (in vitro). This research used an
experimental method with a Completely Randomized Design (CRD). There
are 3 kind of treatments namely: P0 (sugarcane tops without fermentation
and additive), P1 (sugarcane tops silage added with molasses 3%), P2
(sugarcane tops silage added with molasses 3% and Lactobacillus sp 0,5%)
and each treatments was replicated 6 times. The observed parameters were
total bacteria and protozoa population of sheep rumen liquid (In Vitro). The
data were analyzed by variant analysis and followed by Contrast
Orthogonal Test. The result of research indicated that molasses and
Lactobacillus sp addition had significant effect on bacteria rumen, but had
no effect on protozoa rumen. The additions of molasses 3% on sugarcane
tops ensilage (P1) have the best result on total population of bacteria.
Key words: Sugarcane Tops, Silage, Bacteria, Protozoa, in vitro
iv