J. Roca et al. Animal Reproduction Science 64 2000 103–112 107
3. Results
3.1. Experiment 1 The effects of storage time at 15
◦
C on the viability of spermatozoa diluted in the four Tris-buffer extenders are presented in Fig. 1. Regarding all sperm parameters, the viability
of spermatozoa declined with time of storage P 0.05, irrespective of the extender used. There were interactions between extender and time of storage P 0.05 on all viability
parameters evaluated. After 96 h of storage, TTG provided significantly P 0.05 lower sperm viability than the other Tris-buffer extenders used. When comparing extenders within
the same time of storage, no significant differences P 0.05 were found between sper- matozoa examined directly after dilution 0 h with regard to all sperm viability parameters.
However, TSM and PMI at 24 h and TSM, FPM and PMI at 48 h were lower P 0.05 for spermatozoa in TTG than for spermatozoa diluted in the other Tris-buffer extenders. By
72 h, TSM, FPM and PMI were higher P 0.05 for spermatozoa diluted in TCG than in TTG. Spermatozoa examined at 96 h, showed the highest P 0.05 TSM, FPM and PMI
when diluted in TCG. Extender had no effect on the percentage of NAR P 0.05.
Fig. 1. Mean ±S.E.M. percentage of total sperm motility a, forward progressive motility b and percentages of sperm with plasma membrane integrity c and normal acrosome d of rabbit spermatozoa at different times of
preservation at 15
◦
C after dilution in Tris-citric-glucose
h
, Tris-citric-fructose
r
, TES-Tris-glucose
s
and TES-Tris-fructose
m
extenders. Means marked with asterisk are significantly different
∗
P 0.05;
∗∗
P 0.01.
108 J. Roca et al. Animal Reproduction Science 64 2000 103–112
3.2. Experiment 2 Inseminations with semen diluted in TCG extender were made on a total of 3713 does.
The overall conception and farrowing rates were 77.1±0.7 and 70.4±0.7, respectively, and the mean litter size was 7.6 ± 0.1. Table 2 shows the fertility results according to the time
of semen storage. Since the physiological condition of does had a significant effect on the fertility results P 0.001, the does inseminated in each semen storage time were grouped
according to their physiological condition. There was no interaction between time of semen storage and physiological condition of does P 0.05 in any of fertility parameters
evaluated. There were no significant differences P 0.05 in either the conception rate or the farrowing rate or the litter size between the different semen storage times. Regardless
of semen storage time, lactating and nulliparous does showed the highest conception and farrowing rates P 0.01 and primiparous the lowest P 0.05.
4. Discussion
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