UJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN JINTEN (Coleus amboinicus Lour.) TERHADAP BAKTERI Methicillin Resistant Staphylococcus aureus (MRSA) DAN Pseudomonas aeruginosa Multi Resistant (PAMR) DENG.

ABSTRAK

Salah satu tumbuhan di Indonesia yang memiliki kandungan minyak atsiri sebagai
antibakteri adalah daun jinten (Coleus amboinicus Lour.). Penelitian sebelumnya
menunjukkan bahwa minyak atsiri daun jinten mempunyai aktivitas antibakteri
yang kuat terhadap Staphylococcus aureus dan Pseudomonas aeruginosa tetapi
belum pernah dilaporkan untuk bakteri Staphylococcus aureus dan Pseudomonas
aeruginosa yang resisten. Penelitian ini dilakukan untuk mengetahui aktivitas

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antibakteri minyak atsiri daun jinten (Coleus amboinicus Lour.) terhadap bakteri
Methicillin Resistant Staphylococcus aureus (MRSA) dan bakteri Pseudomonas
aeruginosa Multi Resistant (PAMR), menentukan konsentrasi hambat tumbuh

minimum (KHTM), dan menentukan konsentrasi bunuh minimum (KBM).
Pengujian aktivitas antibakteri, penentuan KHTM dan KBM dilakukan dengan
metode mikrodilusi. Hasil pengujian aktivitas antibakteri minyak atsiri daun jinten
menunjukkan bahwa minyak atsiri daun jinten memiliki aktivitas antibakteri
terhadap bakteri resisten tersebut. KHTM minyak atsiri daun jinten terhadap
MRSA terletak pada konsentrasi 750 µg/mL dan KBM terletak pada konsentrasi

1000 µg/mL. KHTM minyak atsiri daun jinten terhadap bakteri PAMR terletak
pada konsentrasi 375 µg/mL, dan KBM terletak pada konsentrasi 750 µg/mL.
Hasil Gas chromatography–mass spectrometry (GCMS) menunjukkan bahwa
minyak atsiri daun jinten mengandung Carvacrol, Caryophyllene, Bergamotene,

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dan α Humulene.

Kata kunci : Coleus amboinicus Lour., Minyak atsiri, Antibakteri, Methicillin
Resistant Staphylococcus aureus, Pseudomonas aeruginosa Multi
Resistant.

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ABSTRACT

One of the plants in Indonesia that contains oil as an antibacterial is Jinten leaves
(Coleus amboinicus Lour.). Previous research showed that essential oil of Jinten
leaves has a strong antibacterial activity against Staphylococcus aureus and
Pseudomonas aeruginosa but has not been reported for the bacteria
Staphylococcus aureus resistant and Pseudomonas aeruginosa resistant. This
research was already conducted to determine the antibacterial activity of Jinten

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leaves essential oil against the Methicillin Resistant Staphylococcus aureus
(MRSA) bacteria and Pseudomonas aeruginosa Multi Resistant (PAMR) bacteria,
determine the Minimum Inhibitory Concentration (MIC), and determine the
Minimum Bactericidal Concentration (MBC). Test for antibacterial activity, MIC
and MBC determined by microdilution method. The results revealed that the
essential oil of Jinten leaves have antibacterial activity against these resistant
bacteria. The MIC of the Jinten leaves essential oil to MRSA was in concentration
of 750 µg/mL and MBC was in concentration of 1000 µg/mL. MIC of the Jinten
leaves essential oil to PAMR bacteria was in concentration 375 µg/mL, and MBC

was in concentration of 750 µg/mL. Gas chromatography-mass spectrometry
(GCMS) result showed that the Jinten leaves contain Carvacrol, Caryophyllene,
Bergamotene, and α Humulene.

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Keywords: Coleus amboinicus Lour., Essential oil, Antibacteria, Methicillin
Resistant Staphylococcus aureus, Pseudomonas aeruginosa Multi
Resistant.

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