Aktivitas Antifertilitas Ekstrak Etanol Daun Jambu Biji (Psidium Guajava L.) Berdasarkan Analisis Semen Dan Tampilan Imunohistokimia Cyclooxygenase-2 Pada Testis Mencit (Mus Musculus L.)

Lampiran 1. Hasil Uji Statistik
Multiple Comparisons
Bonferroni
Dependent

(I)

(J)

Variable

Kelomp

Kelompok

Mean Difference

Std.

(I-J)

Error

Sig.

ok
Jumlah

Kontrol

sperma

P3

Viabilitas

Kontrol

sperma

,000

71,6908

86,8126

82,80667

2,58305

,000

75,2458

90,3676

86,16667

*

2,58305

,000

78,6058

93,7276

-79,25167*

2,58305

,000

-86,8126

-71,6908

P2

3,55500

2,58305

1,000

-4,0059

11,1159

P3

6,91500

2,58305

,087

-,6459

14,4759

*

2,58305

,000

-90,3676

-75,2458

P1

-3,55500

2,58305

1,000

-11,1159

4,0059

P3

3,36000

2,58305

1,000

-4,2009

10,9209

*

2,58305

,000

-93,7276

-78,6058

P1

-6,91500

2,58305

,087

-14,4759

,6459

P2

-3,36000

2,58305

1,000

-10,9209

4,2009

P1

-58,75333

*

1,21572

,000

-62,3119

-55,1948

-62,63833

*

1,21572

,000

-66,1969

-59,0798

-63,89000

*

1,21572

,000

-67,4486

-60,3314

58,75333

*

1,21572

,000

55,1948

62,3119

-3,88500

*

1,21572

,027

-7,4436

-,3264

-5,13667

*

1,21572

,002

-8,6952

-1,5781

62,63833

*

1,21572

,000

59,0798

66,1969

P1

3,88500*

1,21572

,027

,3264

7,4436

P3

-1,25167

1,21572

1,000

-4,8102

2,3069

63,89000

*

1,21572

,000

60,3314

67,4486

5,13667

*

1,21572

,002

1,5781

8,6952

P2

1,25167

1,21572

1,000

-2,3069

4,8102

P1

-52,05333

*

1,42578

,000

-56,2268

-47,8799

-59,60833

*

1,42578

,000

-63,7818

-55,4349

-64,55333

*

1,42578

,000

-68,7268

-60,3799

52,05333*

1,42578

,000

47,8799

56,2268

-7,55500

*

1,42578

,000

-11,7284

-3,3816

-12,50000

*

1,42578

,000

-16,6734

-8,3266

59,60833

*

1,42578

,000

55,4349

63,7818

7,55500

*

1,42578

,000

3,3816

11,7284

-4,94500

*

1,42578

,015

-9,1184

-,7716

Kontrol

Kontrol

Kontrol

Kontrol
P2
P3

P3

Kontrol

Kontrol
P1

Morfologi

Kontrol

sperma

P2
P3
P1

Kontrol
P2
P3

P2

Bound

2,58305

P3

P2

Bound

*

P2

P1

Upper

79,25167

P1

P3

P2

Lower

*

P2

P1

95% Confidence Interval

Kontrol
P1
P3

-82,80667

-86,16667

P3

64,55333*

1,42578

,000

60,3799

68,7268

*

1,42578

,000

8,3266

16,6734

P2

4,94500*

1,42578

,015

,7716

9,1184

P1

73,19500

*

1,10325

,000

69,9657

76,4243

74,44500

*

1,10325

,000

71,2157

77,6743

77,33333

*

1,10325

,000

74,1040

80,5627

-73,19500

*

1,10325

,000

-76,4243

-69,9657

P2

1,25000

1,10325

1,000

-1,9793

4,4793

P3

4,13833

*

1,10325

,008

,9090

7,3677

-74,44500

*

1,10325

,000

-77,6743

-71,2157

P1

-1,25000

1,10325

1,000

-4,4793

1,9793

P3

2,88833

1,10325

,099

-,3410

6,1177

-77,33333

*

1,10325

,000

-80,5627

-74,1040

-4,13833

*

1,10325

,008

-7,3677

-,9090

P2

-2,88833

1,10325

,099

-6,1177

,3410

P1

-7,77667

*

1,25938

,000

-11,4630

-4,0903

-6,88833

*

1,25938

,000

-10,5747

-3,2020

P3

-2,63667

1,25938

,295

-6,3230

1,0497

Kontrol

7,77667*

1,25938

,000

4,0903

11,4630

P2

,88833

1,25938

1,000

-2,7980

4,5747

P3

5,14000

*

1,25938

,003

1,4536

8,8264

6,88833

*

1,25938

,000

3,2020

10,5747

P1

-,88833

1,25938

1,000

-4,5747

2,7980

P3

*

1,25938

,018

,5653

7,9380

2,63667

1,25938

,295

-1,0497

6,3230

P1

-5,14000

*

1,25938

,003

-8,8264

-1,4536

P2

-4,25167*

1,25938

,018

-7,9380

-,5653

P1

-25,13667

*

1,29491

,000

-28,9270

-21,3463

-25,19167

*

1,29491

,000

-28,9820

-21,4013

-26,91333

*

1,29491

,000

-30,7037

-23,1230

25,13667

*

1,29491

,000

21,3463

28,9270

P2

-,05500

1,29491

1,000

-3,8454

3,7354

P3

-1,77667

1,29491

1,000

-5,5670

2,0137

*

1,29491

,000

21,4013

28,9820

P1

,05500

1,29491

1,000

-3,7354

3,8454

P3

-1,72167

1,29491

1,000

-5,5120

2,0687

*

1,29491

,000

23,1230

30,7037

P1

1,77667

1,29491

1,000

-2,0137

5,5670

P2

1,72167

1,29491

1,000

-2,0687

5,5120

P1

*

1,27282

,000

-44,2824

-36,8310

Kontrol
P1

Motilitas a

Kontrol

P2
P3
P1

P2

P3

Kontrol

Kontrol

Kontrol
P1

Motilitas b

Kontrol

P2

P1

P2

P3

Motilitas c

Kontrol

Kontrol

Kontrol

P2
P3
P1

P2

P3

Motilitas d

Kontrol

Kontrol

Kontrol

Kontrol

12,50000

4,25167

25,19167

26,91333

-40,55667

P1

P2

P3

P2

-42,63833*

1,27282

,000

-46,3640

-38,9126

P3

*

1,27282

,000

-51,7824

-44,3310

40,55667*

1,27282

,000

36,8310

44,2824

P2

-2,08167

1,27282

,706

-5,8074

1,6440

P3

-7,50000

*

1,27282

,000

-11,2257

-3,7743

42,63833

*

1,27282

,000

38,9126

46,3640

P1

2,08167

1,27282

,706

-1,6440

5,8074

P3

-5,41833

*

1,27282

,002

-9,1440

-1,6926

48,05667

*

1,27282

,000

44,3310

51,7824

P1

7,50000

*

1,27282

,000

3,7743

11,2257

P2

5,41833*

1,27282

,002

1,6926

9,1440

-48,05667

Kontrol

Kontrol

Kontrol

*. The mean difference is significant at the 0.05 level.

Bootstrap for Multiple Comparisons
Bonferroni
Dependent

(I)

(J)

Variable

Kelompok

Kelompok

Bootstrapa

Mean
Difference

Bias

Std. Error

(I-J)
Jumlah

Kontrol

sperma

P1

P2

P3

Viabilitas

Kontrol

sperma

P1

P2

P3

95% Confidence Interval
Lower

b

85,72390b

79,25167

,05777

P2

82,80667

,08108c

2,74974c

77,46679c

88,31597c

P3

86,16667

,01088b

2,77760b

80,38125b

91,63910b

-79,25167

-,05777b

3,44718b

-85,72390b

-72,16517b

P2

3,55500

,02440c

2,31988c

-,73120c

8,18197c

P3

6,91500

-,04689b

2,37295b

2,48785b

11,69491b

-82,80667

-,08108c

2,74974c

-88,31597c

-77,46679c

P1

-3,55500

-,02440c

2,31988c

-8,18197c

,73120c

P3

3,36000

-,06735c

1,16310c

,65041c

5,38532c

-86,16667

-,01088b

2,77760b

-91,63910b

-80,38125b

P1

-6,91500

,04689b

2,37295b

-11,69491b

-2,48785b

P2

-3,36000

,06735c

1,16310c

-5,38532c

-,65041c

P1

-58,75333

,07305b

1,51240b

-60,98779b

-55,36001b

P2

-62,63833

,00762c

,48581c

-63,50993c

-61,56222c

P3

-63,89000

,02030b

,61784b

-65,22884b

-62,74256b

Kontrol

58,75333

-,07305b

1,51240b

55,36001b

60,98779b

P2

-3,88500

-,06781c

1,55758c

-7,37701c

-1,39104c

P3

-5,13667

-,05274b

1,62197b

-8,71543b

-2,53206b

Kontrol

62,63833

-,00762c

,48581c

61,56222c

63,50993c

P1

3,88500

,06781c

1,55758c

1,39104c

7,37701c

P3

-1,25167

,01187c

,75249c

-2,91990c

,09894c

Kontrol

63,89000

-,02030b

,61784b

62,74256b

65,22884b

Kontrol

Kontrol

72,16517

Upper
b

P1

Kontrol

3,44718

b

Morfologi

Kontrol

sperma

P1

P2

P3

Motilitas a

Kontrol

P1

P2

P3

Motilitas b

Kontrol

P1

P2

P3

Motilitas c

Kontrol

P1

5,13667

,05274b

1,62197b

2,53206b

8,71543b

P2

1,25167

-,01187c

,75249c

-,09894c

2,91990c

P1

-52,05333

,10130b

1,65886b

-55,38747b

-48,83533b

P2

-59,60833

-,04522c

,99073c

-61,66210c

-57,75783c

P3

-64,55333

,00970b

,69532b

-65,93259b

-63,21861b

Kontrol

52,05333

-,10130b

1,65886b

48,83533b

55,38747b

P2

-7,55500

-,14877c

1,86835c

-11,52325c

-4,32350c

P3

-12,50000

-,09160b

1,69663b

-15,90341b

-9,31359b

59,60833

,04522c

,99073c

57,75783c

61,66210c

P1

7,55500

,14877c

1,86835c

4,32350c

11,52325c

P3

-4,94500

,05604c

1,03183c

-6,93624c

-2,84937c

Kontrol

64,55333

-,00970b

,69532b

63,21861b

65,93259b

P1

12,50000

,09160b

1,69663b

9,31359b

15,90341b

P2

4,94500

-,05604c

1,03183c

2,84937c

6,93624c

P1

73,19500

,04550b

1,40011b

70,41449b

75,85553b

P2

74,44500

,03191c

1,32177c

71,70934c

77,08757c

P3

77,33333

,02569b

1,33488b

74,49201b

79,99065b

-73,19500

-,04550b

1,40011b

-75,85553b

-70,41449b

P2

1,25000

-,01345c

,69136c

-,00239c

2,72154c

P3

4,13833

-,01981b

,73058b

2,79131b

5,63723b

-74,44500

-,03191c

1,32177c

-77,08757c

-71,70934c

P1

-1,25000

,01345c

,69136c

-2,72154c

,00239c

P3

2,88833

-,00684c

,50039c

1,93719c

3,87337c

-77,33333

-,02569b

1,33488b

-79,99065b

-74,49201b

P1

-4,13833

,01981b

,73058b

-5,63723b

-2,79131b

P2

-2,88833

,00684c

,50039c

-3,87337c

-1,93719c

P1

-7,77667

,01232b

1,04190b

-9,75961b

-5,64842b

P2

-6,88833

-,08751c

1,29697c

-9,79000c

-4,63600c

P3

-2,63667

-,01786b

1,35852b

-5,41333b

,03549b

7,77667

-,01232b

1,04190b

5,64842b

9,75961b

P2

,88833

-,10006c

1,07221c

-1,47235c

2,77970c

P3

5,14000

-,03018b

1,12609b

2,92000b

7,41551b

Kontrol

6,88833

,08751c

1,29697c

4,63600c

9,79000c

P1

-,88833

,10006c

1,07221c

-2,77970c

1,47235c

P3

4,25167

,07015c

1,39312c

1,69023c

7,18990c

Kontrol

2,63667

,01786b

1,35852b

-,03549b

5,41333b

P1

-5,14000

,03018b

1,12609b

-7,41551b

-2,92000b

P2

-4,25167

-,07015c

1,39312c

-7,18990c

-1,69023c

P1

-25,13667

,00675b

1,02836b

-27,14806b

-23,06344b

P2

-25,19167

,11297c

1,34651c

-27,66500c

-22,35973c

Kontrol

Kontrol

Kontrol

Kontrol

Kontrol

-26,91333

,01005b

,76277b

-28,46111b

-25,42168b

25,13667

-,00675b

1,02836b

23,06344b

27,14806b

P2

-,05500

,10506c

1,57088c

-2,90673c

3,11952c

P3

-1,77667

,00330b

1,14854b

-4,04158b

,54523b

Kontrol

25,19167

-,11297c

1,34651c

22,35973c

27,66500c

P1

,05500

-,10506c

1,57088c

-3,11952c

2,90673c

P3

-1,72167

-,10243c

1,43293c

-4,76901c

,87398c

Kontrol

26,91333

-,01005b

,76277b

25,42168b

28,46111b

P1

1,77667

-,00330b

1,14854b

-,54523b

4,04158b

P2

1,72167

,10243c

1,43293c

-,87398c

4,76901c

P1

-40,55667

-,05505b

1,27936b

-43,29003b

-38,33350b

P2

-42,63833

-,04757c

1,11827c

-44,83435c

-40,51216c

P3

-48,05667

-,00828b

1,07885b

-50,30583b

-46,01088b

Kontrol

40,55667

,05505b

1,27936b

38,33350b

43,29003b

P2

-2,08167

,00871c

1,31485c

-4,58445c

,62750c

P3

-7,50000

,04677b

1,32146b

-9,99171b

-4,83217b

Kontrol

42,63833

,04757c

1,11827c

40,51216c

44,83435c

P1

2,08167

-,00871c

1,31485c

-,62750c

4,58445c

P3

-5,41833

,03894c

1,23738c

-7,91971c

-2,92162c

Kontrol

48,05667

,00828b

1,07885b

46,01088b

50,30583b

P1

7,50000

-,04677b

1,32146b

4,83217b

9,99171b

P2

5,41833

-,03894c

1,23738c

2,92162c

7,91971c

P3
P1

P2

P3

Motilitas d

Kontrol

P1

P2

P3

Kontrol

a. Unless otherwise noted, bootstrap results are based on 1000 bootstrap samples
b. Based on 969 samples
c. Based on 968 samples

Lampiran 2 Dokumentasi Penelitian

Pohon Jambu Biji

Penyaringan setelah Maserasi

Improved Neubauer

Maserasi Daun Jambu Biji

Penentuan Kadar Air

Gelas Arloji

Disicting set

Pengambilan Organ Testis

Pembedahan mencit

Pemeliharaan Mus musculus L.