Efek Ekstrak Buncis (Phaseolus Vulgaris L) Terhadap Kadar Ldl Dan Oxldl Serta Kaitannya Dengan Polimorfisme Gen Lox-1 3’utr188c T Sebagai Upaya Preventif Risiko Aterosklerosis
185
LAMPIRAN
Universitas Sumatera Utara
186
Universitas Sumatera Utara
187
Lembar Penjelasan Kepada Calon Subjek Penelitian
Dengan hormat,
Saya dr Shahrul Rahman, Sp.PD saat ini sedang menjalani pendidikan S3
kedokteran program studi S3 ilmu kedokteran fakultas kedokteran
Universitas Sumatera Utara. Saya sedang melakukan penelitian yang
berjudul EFEK EKSTRAK BUNCIS (Phaseolus vulgaris L) TERHADAP
KADAR
LDL
POLIMORFISME
DAN
OxLDL
GEN
SERTA
KAITANNYA
3‟UTR188CT
LOX-1
SEBAGAI
DENGAN
UPAYA
PREVENTIF RISIKO ATEROSKLEROSIS.
Aterosklerosis adalah merupakan gangguan pembuluh darah dijantung
yang akan dapat berakibat fatal pada penderitanya. Oleh karena itu,
pencegahan
terhadap
terjadi
dan
berkembangnya
aterosklerosis
merupakan hal yang penting.
Buncis yang sudah dikenal sebagai sayuran yang sering dikonsumsi
sehari-hari,
diyakini
mempunyai
efek
yang
dapat
mencegah
berkembangnya proses pengerasan dinding pembuluh darah. Sehingga
tujuan penelitian ini selain untuk mengetahui keberadaan gen LOX-1
3‟UTR188CT, juga untuk mendapatkan efek pencegahan dari buncis
terhadap berkembangnya proses tersebut. Manfaat bagi peserta adalah
selain dapat mengetahui parameter laboratorium juga dapat mengetahui
efek baik buncis.
Adapun proses penelitian yang Bapak/Ibu/Sdr/i akan ikuti dalam penelitian
ini adalah:
Universitas Sumatera Utara
188
1. Pengambilan darah dengan menggunakan jarum suntik sebanyak
10 cc (1 jarum suntik 10 cc penuh)
2. Tujuan pengambilan darah adalah untuk melakukan pemeriksaan
profil lemak, kadar OxLDL dan polimorfisme gen 3‟UTR188CT
3. Pengambilan darah akan dilakukan sebanyak 2 kali, yaitu periode
sebelum perlakuan dan sesudah perlakuan
4. Darah yang diambil akan diperiksa pada Laboratorium Klinik
Prodia Medan dan Eijkman Institute, Jakarta.
Sebagai informasi, efek sampng pengambilan darah hampir tidak ada
karena dilakukan sesuai dengan prosedur pengambilan darah yang steril.
Jika Bapak/Ibu/Sdr/i mengalami efek samping seperti pembengkakan di
daerah tempat pengambilan darah, maka peneliti wajib memberikan
pengobatan yaitu dengan menekan dan mengompres dengan kain kasa
yang telah dibasahi oleh alkohol 70%.
Partisipasi Bapak/Ibu/Sdr/i bersifat suka rela dan tanpa paksaan. Setiap
data yang ada dalam penelitian ini dirahasiakan dan digunakan untuk
kepentingan penelitian. Untuk mengikuti penelitian ini Bapak/Ibu/Sdr/i
tidak akan dipungut biaya apapun.
Bila Bapak/Ibu/Sdr/i bersedia untuk menjadi subyek penelitian, mohon
untuk mengisi dan menandatangani lembar persetujuan yang telah kami
sediakan.
Jika Bapak/Ibu/Sdr/i membutuhkan penjelasan lebih lanjut, maka dapat
menghubung saya di no telp: 08126093740.
Universitas Sumatera Utara
189
Terima kasih saya ucapkan kepada Bapak/Ibu/Sdr/i yang telah bersedia
ikut berpartisipasi pada penelitian ini. Keikutsertaan Bapak/Ibu/Sdr/i dalam
penelitian ini akan menyumbangkan sesuatu yang berguna bagi ilmu
pengetahuan.
Medan, ....................2013
dr Shahrul Rahman, Sp.PD
Universitas Sumatera Utara
190
SURAT PERSETUJUAN SETELAH PENJELASAN
(INFORMED CONSENT)
Saya yang bertanda tangan di bawah ini :
Nama
: ………………………………………………
Alamat
: ………………………………………………
Umur
: …... tahun
Jenis Kelamin : Pria / Wanita
Setelah mempelajari dan mendapat penjelasan yang sejelas-jelasnya
mengenai penelitian yang berjudul : EFEK EKSTRAK BUNCIS (Phaseolus
vulgaris L) TERHADAP KADAR LDL DAN OxLDL SERTA KAITANNYA
DENGAN POLIMORFISME GEN LOX-1 3‟UTR188CT SEBAGAI UPAYA
PREVENTIF RISIKO ATEROSKLEROSIS dan setelah mengetahui dan
menyadari sepenuhnya tentang risiko efek samping yang mungkin akan
terjadi, dengan ini saya menyatakan bahwa saya bersedia mengikuti
prosedur penelitian dengan sadar, sukarela dan tanpa paksaan untuk
menjadi subyek dalam penelitian ini.
Medan, ....................2013
Yang membuat pernyataan
( ........................................... )
Peneliti
dr Shahrul Rahman, Sp.PD
Universitas Sumatera Utara
191
Oneway
Descriptives
Umur (tahun)
N
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
Total
Mean
43.00
38.20
44.43
38.78
40.83
9
10
7
9
35
Std. Deviation
4.555
5.308
4.614
5.167
5.426
Std. Error
1.518
1.679
1.744
1.722
.917
95% Confidence Interval for
Mean
Lower Bound
Upper Bound
39.50
46.50
34.40
42.00
40.16
48.70
34.81
42.75
38.96
42.69
Minimum
36
31
37
30
30
Maximum
50
48
50
46
50
Test of Homogeneity of Variances
Umur (tahun)
Levene
Statistic
.122
df1
df2
3
Sig.
.946
31
ANOVA
Umur (tahun)
Between Groups
Within Groups
Total
Sum of
Squares
240.102
760.870
1000.971
df
3
31
34
Mean Square
80.034
24.544
F
3.261
Sig.
.035
Post Hoc Tests
Multiple Comparisons
Dependent Variable: Umur (tahun)
LSD
(I) Pemberian obat
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
(J) Pemberian obat
Dosis 80 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Mean
Difference
Std. Error
(I-J)
4.800*
2.276
-1.429
2.497
4.222
2.335
-4.800*
2.276
-6.229*
2.441
-.578
2.276
1.429
2.497
6.229*
2.441
5.651*
2.497
-4.222
2.335
.578
2.276
-5.651*
2.497
Sig.
.043
.571
.080
.043
.016
.801
.571
.016
.031
.080
.801
.031
95% Confidence Interval
Lower Bound
Upper Bound
.16
9.44
-6.52
3.66
-.54
8.99
-9.44
-.16
-11.21
-1.25
-5.22
4.06
-3.66
6.52
1.25
11.21
.56
10.74
-8.99
.54
-4.06
5.22
-10.74
-.56
*. The mean difference is significant at the .05 level.
Universitas Sumatera Utara
192
Oneway
Descriptives
KGD
N
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
Total
Mean
87.00
87.70
80.00
86.78
85.74
9
10
7
9
35
Std. Deviation
8.675
8.166
7.616
7.032
8.114
Std. Error
2.892
2.582
2.878
2.344
1.372
95% Confidence Interval for
Mean
Lower Bound
Upper Bound
80.33
93.67
81.86
93.54
72.96
87.04
81.37
92.18
82.96
88.53
Minimum
77
76
73
77
73
Maximum
106
107
94
97
107
Test of Homogeneity of Variances
KGD
Levene
Statistic
.037
df1
df2
3
Sig.
.990
31
ANOVA
KGD
Between Groups
Within Groups
Total
Sum of
Squares
293.030
1945.656
2238.686
df
3
31
34
Mean Square
97.677
62.763
F
1.556
Sig.
.220
Post Hoc Tests
Multiple Comparisons
Dependent Variable: KGD
LSD
(I) Pemberian obat
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
(J) Pemberian obat
Dosis 80 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Mean
Difference
(I-J)
-.700
7.000
.222
.700
7.700
.922
-7.000
-7.700
-6.778
-.222
-.922
6.778
Std. Error
3.640
3.992
3.735
3.640
3.904
3.640
3.992
3.904
3.992
3.735
3.640
3.992
Sig.
.849
.089
.953
.849
.058
.802
.089
.058
.100
.953
.802
.100
95% Confidence Interval
Lower Bound
Upper Bound
-8.12
6.72
-1.14
15.14
-7.39
7.84
-6.72
8.12
-.26
15.66
-6.50
8.35
-15.14
1.14
-15.66
.26
-14.92
1.36
-7.84
7.39
-8.35
6.50
-1.36
14.92
Universitas Sumatera Utara
193
Oneway
Descriptives
Ureum
N
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
Total
9
10
7
9
35
Mean
20.89
20.80
19.43
18.22
19.89
Std. Deviation
5.622
5.594
2.370
4.577
4.788
Std. Error
1.874
1.769
.896
1.526
.809
95% Confidence Interval for
Mean
Lower Bound
Upper Bound
16.57
25.21
16.80
24.80
17.24
21.62
14.70
21.74
18.24
21.53
Minimum
15
13
16
10
10
Maximum
33
29
22
25
33
Test of Homogeneity of Variances
Ureum
Levene
Statistic
1.685
df1
df2
3
Sig.
.191
31
ANOVA
Ureum
Between Groups
Within Groups
Total
Sum of
Squares
43.784
735.759
779.543
df
3
31
34
Mean Square
14.595
23.734
F
.615
Sig.
.611
Post Hoc Tests
Multiple Comparisons
Dependent Variable: Ureum
LSD
(I) Pemberian obat
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
(J) Pemberian obat
Dosis 80 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Mean
Difference
(I-J)
.089
1.460
2.667
-.089
1.371
2.578
-1.460
-1.371
1.206
-2.667
-2.578
-1.206
Std. Error
2.238
2.455
2.297
2.238
2.401
2.238
2.455
2.401
2.455
2.297
2.238
2.455
Sig.
.969
.556
.254
.969
.572
.258
.556
.572
.627
.254
.258
.627
95% Confidence Interval
Lower Bound
Upper Bound
-4.48
4.65
-3.55
6.47
-2.02
7.35
-4.65
4.48
-3.53
6.27
-1.99
7.14
-6.47
3.55
-6.27
3.53
-3.80
6.21
-7.35
2.02
-7.14
1.99
-6.21
3.80
Universitas Sumatera Utara
194
Oneway
Descriptives
Creatinin
N
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
Total
9
10
7
9
35
Mean
.8367
.8040
.7614
.8289
.8103
Std. Deviation
.25725
.26222
.16088
.17934
.21607
Std. Error
.08575
.08292
.06081
.05978
.03652
95% Confidence Interval for
Mean
Lower Bound
Upper Bound
.6389
1.0344
.6164
.9916
.6126
.9102
.6910
.9667
.7361
.8845
Minimum
.59
.52
.60
.59
.52
Maximum
1.41
1.33
1.10
1.08
1.41
Test of Homogeneity of Variances
Creatinin
Levene
Statistic
.867
df1
df2
3
Sig.
.468
31
ANOVA
Creatinin
Between Groups
Within Groups
Total
Sum of
Squares
.026
1.561
1.587
df
3
31
34
Mean Square
.009
.050
F
.175
Sig.
.912
Post Hoc Tests
Multiple Comparisons
Dependent Variable: Creatinin
LSD
(I) Pemberian obat
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
(J) Pemberian obat
Dosis 80 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Mean
Difference
(I-J)
.03267
.07524
.00778
-.03267
.04257
-.02489
-.07524
-.04257
-.06746
-.00778
.02489
.06746
Std. Error
.10310
.11308
.10578
.10310
.11058
.10310
.11308
.11058
.11308
.10578
.10310
.11308
Sig.
.753
.511
.942
.753
.703
.811
.511
.703
.555
.942
.811
.555
95% Confidence Interval
Lower Bound
Upper Bound
-.1776
.2429
-.1554
.3059
-.2080
.2235
-.2429
.1776
-.1830
.2681
-.2352
.1854
-.3059
.1554
-.2681
.1830
-.2981
.1632
-.2235
.2080
-.1854
.2352
-.1632
.2981
Universitas Sumatera Utara
195
Oneway
Descriptives
Ox-LDL (pre)
N
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
Total
Mean
238.33
144.50
251.14
225.44
210.77
9
10
7
9
35
Std. Deviation
67.067
94.151
58.453
76.054
85.171
Std. Error
22.356
29.773
22.093
25.351
14.397
95% Confidence Interval for
Mean
Lower Bound
Upper Bound
186.78
289.89
77.15
211.85
197.08
305.20
166.98
283.91
181.51
240.03
Minimum
134
44
127
77
44
Maximum
317
277
297
302
317
Test of Homogeneity of Variances
Ox-LDL (pre)
Levene
Statistic
2.444
df1
df2
3
Sig.
.083
31
ANOVA
Ox-LDL (pre)
Between Groups
Within Groups
Total
Sum of
Squares
64102.592
182539.6
246642.2
df
3
31
34
Mean Square
21367.531
5888.374
F
3.629
Sig.
.024
Post Hoc Tests
Multiple Comparisons
Dependent Variable: Ox-LDL (pre)
LSD
(I) Pemberian obat
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
(J) Pemberian obat
Dosis 80 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Mean
Difference
Std. Error
(I-J)
93.833*
35.258
-12.810
38.671
12.889
36.174
-93.833*
35.258
-106.643*
37.816
-80.944*
35.258
12.810
38.671
106.643*
37.816
25.698
38.671
-12.889
36.174
80.944*
35.258
-25.698
38.671
Sig.
.012
.743
.724
.012
.008
.029
.743
.008
.511
.724
.029
.511
95% Confidence Interval
Lower Bound
Upper Bound
21.92
165.74
-91.68
66.06
-60.89
86.67
-165.74
-21.92
-183.77
-29.52
-152.85
-9.04
-66.06
91.68
29.52
183.77
-53.17
104.57
-86.67
60.89
9.04
152.85
-104.57
53.17
*. The mean difference is significant at the .05 level.
Universitas Sumatera Utara
196
Oneway
Descriptives
Ox-LDL (post)
N
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
Total
Mean
265.78
261.50
302.43
283.00
276.31
9
10
7
9
35
Std. Deviation
72.475
54.062
20.582
53.796
54.877
Std. Error
24.158
17.096
7.779
17.932
9.276
95% Confidence Interval for
Mean
Lower Bound
Upper Bound
210.07
321.49
222.83
300.17
283.39
321.46
241.65
324.35
257.46
295.17
Minimum
84
163
276
184
84
Maximum
329
313
331
338
338
Test of Homogeneity of Variances
Ox-LDL (post)
Levene
Statistic
1.244
df1
df2
3
Sig.
.311
31
ANOVA
Ox-LDL (post)
Between Groups
Within Groups
Total
Sum of
Squares
8369.773
94019.770
102389.5
df
3
31
34
Mean Square
2789.924
3032.896
F
.920
Sig.
.443
Post Hoc Tests
Multiple Comparisons
Dependent Variable: Ox-LDL (post)
LSD
(I) Pemberian obat
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
(J) Pemberian obat
Dosis 80 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Mean
Difference
(I-J)
4.278
-36.651
-17.222
-4.278
-40.929
-21.500
36.651
40.929
19.429
17.222
21.500
-19.429
Std. Error
25.304
27.754
25.961
25.304
27.140
25.304
27.754
27.140
27.754
25.961
25.304
27.754
Sig.
.867
.196
.512
.867
.142
.402
.196
.142
.489
.512
.402
.489
95% Confidence Interval
Lower Bound
Upper Bound
-47.33
55.89
-93.25
19.95
-70.17
35.73
-55.89
47.33
-96.28
14.42
-73.11
30.11
-19.95
93.25
-14.42
96.28
-37.18
76.03
-35.73
70.17
-30.11
73.11
-76.03
37.18
Universitas Sumatera Utara
197
NPar Tests (Dosis 40 mg)
One-Sample Kolmogorov-Smirnov Test
a,b
Normal Parameters
Mean
Std. Deviation
N
Kolesterol
(pre)
Kolesterol
(post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida
(pre)
Trigiserida
(post)
Ox-LDL (pre)
Ox-LDL (post)
Most Extreme Differences
Absolute
Positive
Negative
Kolmogorov-Smirnov Z
Asymp. Sig. (2-tailed)
9
214.11
64.449
.237
.110
-.237
.712
.691
9
228.11
48.048
.266
.266
-.190
.799
.547
9
9
9
9
137.67
137.44
45.67
47.44
48.003
28.076
18.527
14.492
.145
.121
.177
.166
.120
.121
.177
.166
-.145
-.106
-.132
-.102
.434
.364
.532
.499
.992
.999
.940
.965
9
158.11
95.241
.189
.189
-.124
.567
.904
9
163.44
86.986
.190
.179
-.190
.571
.900
9
9
238.33
265.78
67.067
72.475
.188
.287
.168
.192
-.188
-.287
.564
.860
.909
.450
a. Test distribution is Normal.
b. Calculated from data.
T-Test
Paired Samples Statistics
Pair
1
Pair
2
Pair
3
Pair
4
Pair
5
Kolesterol (pre)
Kolesterol (post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida (pre)
Trigiserida (post)
Ox-LDL (pre)
Ox-LDL (post)
Mean
214.11
228.11
137.67
137.44
45.67
47.44
158.11
163.44
238.33
265.78
N
Std. Error
Mean
21.483
16.016
16.001
9.359
6.176
4.831
31.747
28.995
22.356
24.158
Std. Deviation
64.449
48.048
48.003
28.076
18.527
14.492
95.241
86.986
67.067
72.475
9
9
9
9
9
9
9
9
9
9
Paired Samples Correlations
N
Pair 1
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) &
Kolesterol (post)
LDL (pre) & LDL (post)
HDL (pre) & HDL (post)
Trigiserida (pre) &
Trigiserida (post)
Ox-LDL (pre) & Ox-LDL
(post)
Correlation
Sig.
9
.852
.004
9
9
.956
.881
.000
.002
9
.947
.000
9
.499
.172
Paired Samples Test
Paired Differences
Std. Deviation
Std. Error
Mean
-14.000
34.406
11.469
-40.447
12.447
-1.221
8
.257
.222
-1.778
22.703
8.969
7.568
2.990
-17.229
-8.672
17.674
5.116
.029
-.595
8
8
.977
.569
-5.333
30.859
10.286
-29.053
18.387
-.518
8
.618
-27.444
70.009
23.336
-81.258
26.369
-1.176
8
.273
Mean
Pair 1
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) Kolesterol (post)
LDL (pre) - LDL (post)
HDL (pre) - HDL (post)
Trigiserida (pre) Trigiserida (post)
Ox-LDL (pre) - Ox-LDL
(post)
95% Confidence
Interval of the
Difference
Lower
Upper
t
df
Sig. (2-tailed)
Universitas Sumatera Utara
198
NPar Tests (Dosis 80 mg)
One-Sample Kolmogorov-Smirnov Test
a,b
Normal Parameters
Mean
Std. Deviation
N
Kolesterol
(pre)
Kolesterol
(post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida
(pre)
Trigiserida
(post)
Ox-LDL (pre)
Ox-LDL (post)
Most Extreme Differences
Absolute
Positive
Negative
Kolmogorov-Smirnov Z
Asymp. Sig. (2-tailed)
10
197.60
36.078
.143
.137
-.143
.454
.986
10
199.20
36.411
.142
.142
-.132
.450
.988
10
10
10
10
125.00
121.20
48.10
45.70
33.400
20.682
10.408
10.853
.183
.147
.154
.198
.115
.106
.154
.198
-.183
-.147
-.143
-.162
.579
.465
.486
.627
.891
.982
.972
.827
10
123.60
71.071
.206
.206
-.167
.653
.787
10
146.60
110.368
.356
.356
-.222
1.126
.158
10
10
144.50
261.50
94.151
54.062
.199
.313
.199
.170
-.178
-.313
.628
.989
.825
.282
a. Test distribution is Normal.
b. Calculated from data.
T-Test
Paired Samples Statistics
Pair
1
Pair
2
Pair
3
Pair
4
Pair
5
Kolesterol (pre)
Kolesterol (post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida (pre)
Trigiserida (post)
Ox-LDL (pre)
Ox-LDL (post)
Mean
197.60
199.20
125.00
121.20
48.10
45.70
123.60
146.60
144.50
261.50
N
Std. Deviation
36.078
36.411
33.400
20.682
10.408
10.853
71.071
110.368
94.151
54.062
10
10
10
10
10
10
10
10
10
10
Std. Error
Mean
11.409
11.514
10.562
6.540
3.291
3.432
22.475
34.902
29.773
17.096
Paired Samples Correlations
N
Pair 1
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) &
Kolesterol (post)
LDL (pre) & LDL (post)
HDL (pre) & HDL (post)
Trigiserida (pre) &
Trigiserida (post)
Ox-LDL (pre) & Ox-LDL
(post)
Correlation
Sig.
10
.726
.017
10
10
.787
.938
.007
.000
10
.712
.021
10
-.340
.336
Paired Samples Test
Paired Differences
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) Kolesterol (post)
LDL (pre) - LDL (post)
HDL (pre) - HDL (post)
Trigiserida (pre) Trigiserida (post)
Ox-LDL (pre) - Ox-LDL
(post)
t
Std. Deviation
Std. Error
Mean
-1.600
26.825
8.483
-20.790
17.590
-.189
9
.855
3.800
2.400
21.369
3.777
6.757
1.194
-11.486
-.302
19.086
5.102
.562
2.009
9
9
.588
.075
-23.000
77.866
24.623
-78.702
32.702
-.934
9
.375
-117.000
123.500
39.054
-205.346
-28.654
-2.996
9
.015
Mean
Pair 1
95% Confidence
Interval of the
Difference
Lower
Upper
df
Sig. (2-tailed)
Universitas Sumatera Utara
199
NPar Tests (Dosis 120 mg)
One-Sample Kolmogorov-Smirnov Test
a,b
Normal Parameters
Mean
Std. Deviation
N
Kolesterol
(pre)
Kolesterol
(post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida
(pre)
Trigiserida
(post)
Ox-LDL (pre)
Ox-LDL (post)
Most Extreme Differences
Absolute
Positive
Negative
Kolmogorov-Smirnov Z
Asymp. Sig. (2-tailed)
7
214.71
50.255
.229
.179
-.229
.605
.858
7
215.71
31.952
.221
.164
-.221
.585
.883
7
7
7
7
139.57
138.57
50.29
47.71
45.048
33.040
6.157
4.821
.163
.162
.216
.181
.145
.162
.216
.168
-.163
-.094
-.155
-.181
.431
.428
.572
.478
.992
.993
.899
.976
7
125.86
42.807
.248
.162
-.248
.657
.781
7
144.43
47.233
.126
.126
-.096
.334
1.000
7
7
251.14
302.43
58.453
20.582
.288
.212
.216
.212
-.288
-.187
.763
.562
.606
.910
a. Test distribution is Normal.
b. Calculated from data.
T-Test
Paired Samples Statistics
Pair
1
Pair
2
Pair
3
Pair
4
Pair
5
Kolesterol (pre)
Kolesterol (post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida (pre)
Trigiserida (post)
Ox-LDL (pre)
Ox-LDL (post)
Mean
214.71
215.71
139.57
138.57
50.29
47.71
125.86
144.43
251.14
302.43
N
7
7
7
7
7
7
7
7
7
7
Std. Deviation
50.255
31.952
45.048
33.040
6.157
4.821
42.807
47.233
58.453
20.582
Std. Error
Mean
18.995
12.077
17.026
12.488
2.327
1.822
16.180
17.852
22.093
7.779
Paired Samples Correlations
N
Pair 1
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) &
Kolesterol (post)
LDL (pre) & LDL (post)
HDL (pre) & HDL (post)
Trigiserida (pre) &
Trigiserida (post)
Ox-LDL (pre) & Ox-LDL
(post)
Correlation
Sig.
7
.870
.011
7
7
.852
.857
.015
.014
7
.878
.009
7
.330
.469
Paired Samples Test
Paired Differences
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) Kolesterol (post)
LDL (pre) - LDL (post)
HDL (pre) - HDL (post)
Trigiserida (pre) Trigiserida (post)
Ox-LDL (pre) - Ox-LDL
(post)
t
Std. Deviation
Std. Error
Mean
-1.000
27.459
10.379
-26.395
24.395
-.096
6
.926
1.000
2.571
24.194
3.207
9.144
1.212
-21.375
-.395
23.375
5.538
.109
2.121
6
6
.916
.078
-18.571
22.619
8.549
-39.491
2.348
-2.172
6
.073
-51.286
55.190
20.860
-102.328
-.244
-2.459
6
.049
Mean
Pair 1
95% Confidence
Interval of the
Difference
Lower
Upper
df
Sig. (2-tailed)
Universitas Sumatera Utara
200
NPar Tests (Plasebo)
One-Sample Kolmogorov-Smirnov Test
a,b
Normal Parameters
Mean
Std. Deviation
N
Kolesterol
(pre)
Kolesterol
(post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida
(pre)
Trigiserida
(post)
Ox-LDL (pre)
Ox-LDL (post)
Most Extreme Differences
Absolute
Positive
Negative
Kolmogorov-Smirnov Z
Asymp. Sig. (2-tailed)
9
187.22
27.833
.199
.199
-.169
.598
.868
9
201.89
26.582
.172
.129
-.172
.517
.952
9
9
9
9
118.44
127.11
43.44
44.78
25.279
24.700
5.790
10.390
.177
.237
.136
.308
.121
.237
.107
.308
-.177
-.222
-.136
-.199
.531
.710
.408
.923
.940
.694
.996
.362
9
125.56
72.236
.236
.236
-.127
.707
.699
9
124.56
68.608
.214
.214
-.197
.642
.805
9
9
225.44
283.00
76.054
53.796
.259
.196
.157
.153
-.259
-.196
.776
.589
.584
.879
a. Test distribution is Normal.
b. Calculated from data.
T-Test
Paired Samples Statistics
Pair
1
Pair
2
Pair
3
Pair
4
Pair
5
Mean
187.22
201.89
118.44
127.11
43.44
44.78
125.56
124.56
225.44
283.00
Kolesterol (pre)
Kolesterol (post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida (pre)
Trigiserida (post)
Ox-LDL (pre)
Ox-LDL (post)
N
Std. Error
Mean
9.278
8.861
8.426
8.233
1.930
3.463
24.079
22.869
25.351
17.932
Std. Deviation
27.833
26.582
25.279
24.700
5.790
10.390
72.236
68.608
76.054
53.796
9
9
9
9
9
9
9
9
9
9
Paired Samples Correlations
N
Pair 1
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) &
Kolesterol (post)
LDL (pre) & LDL (post)
HDL (pre) & HDL (post)
Trigiserida (pre) &
Trigiserida (post)
Ox-LDL (pre) & Ox-LDL
(post)
Correlation
Sig.
9
.432
.245
9
9
.583
.733
.099
.025
9
.943
.000
9
-.268
.486
Paired Samples Test
Paired Differences
Std. Deviation
Std. Error
Mean
-14.667
29.009
9.670
-36.965
7.631
-1.517
8
.168
-8.667
-1.333
22.814
7.297
7.605
2.432
-26.203
-6.943
8.870
4.276
-1.140
-.548
8
8
.287
.599
1.000
24.005
8.002
-17.452
19.452
.125
8
.904
-57.556
104.261
34.754
-137.697
22.586
-1.656
8
.136
Mean
Pair 1
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) Kolesterol (post)
LDL (pre) - LDL (post)
HDL (pre) - HDL (post)
Trigiserida (pre) Trigiserida (post)
Ox-LDL (pre) - Ox-LDL
(post)
95% Confidence
Interval of the
Difference
Lower
Upper
t
df
Sig. (2-tailed)
Universitas Sumatera Utara
201
Kebun Buncis di Berastagi
Laboratorium Obat Tradisional Fakultas Farmasi USU
Universitas Sumatera Utara
202
Penyuluhan dan Pengambilan Darah di Rumah Sakit Putri Hijau
Universitas Sumatera Utara
203
Eijkman Institute Jakarta
Universitas Sumatera Utara
LAMPIRAN
Universitas Sumatera Utara
186
Universitas Sumatera Utara
187
Lembar Penjelasan Kepada Calon Subjek Penelitian
Dengan hormat,
Saya dr Shahrul Rahman, Sp.PD saat ini sedang menjalani pendidikan S3
kedokteran program studi S3 ilmu kedokteran fakultas kedokteran
Universitas Sumatera Utara. Saya sedang melakukan penelitian yang
berjudul EFEK EKSTRAK BUNCIS (Phaseolus vulgaris L) TERHADAP
KADAR
LDL
POLIMORFISME
DAN
OxLDL
GEN
SERTA
KAITANNYA
3‟UTR188CT
LOX-1
SEBAGAI
DENGAN
UPAYA
PREVENTIF RISIKO ATEROSKLEROSIS.
Aterosklerosis adalah merupakan gangguan pembuluh darah dijantung
yang akan dapat berakibat fatal pada penderitanya. Oleh karena itu,
pencegahan
terhadap
terjadi
dan
berkembangnya
aterosklerosis
merupakan hal yang penting.
Buncis yang sudah dikenal sebagai sayuran yang sering dikonsumsi
sehari-hari,
diyakini
mempunyai
efek
yang
dapat
mencegah
berkembangnya proses pengerasan dinding pembuluh darah. Sehingga
tujuan penelitian ini selain untuk mengetahui keberadaan gen LOX-1
3‟UTR188CT, juga untuk mendapatkan efek pencegahan dari buncis
terhadap berkembangnya proses tersebut. Manfaat bagi peserta adalah
selain dapat mengetahui parameter laboratorium juga dapat mengetahui
efek baik buncis.
Adapun proses penelitian yang Bapak/Ibu/Sdr/i akan ikuti dalam penelitian
ini adalah:
Universitas Sumatera Utara
188
1. Pengambilan darah dengan menggunakan jarum suntik sebanyak
10 cc (1 jarum suntik 10 cc penuh)
2. Tujuan pengambilan darah adalah untuk melakukan pemeriksaan
profil lemak, kadar OxLDL dan polimorfisme gen 3‟UTR188CT
3. Pengambilan darah akan dilakukan sebanyak 2 kali, yaitu periode
sebelum perlakuan dan sesudah perlakuan
4. Darah yang diambil akan diperiksa pada Laboratorium Klinik
Prodia Medan dan Eijkman Institute, Jakarta.
Sebagai informasi, efek sampng pengambilan darah hampir tidak ada
karena dilakukan sesuai dengan prosedur pengambilan darah yang steril.
Jika Bapak/Ibu/Sdr/i mengalami efek samping seperti pembengkakan di
daerah tempat pengambilan darah, maka peneliti wajib memberikan
pengobatan yaitu dengan menekan dan mengompres dengan kain kasa
yang telah dibasahi oleh alkohol 70%.
Partisipasi Bapak/Ibu/Sdr/i bersifat suka rela dan tanpa paksaan. Setiap
data yang ada dalam penelitian ini dirahasiakan dan digunakan untuk
kepentingan penelitian. Untuk mengikuti penelitian ini Bapak/Ibu/Sdr/i
tidak akan dipungut biaya apapun.
Bila Bapak/Ibu/Sdr/i bersedia untuk menjadi subyek penelitian, mohon
untuk mengisi dan menandatangani lembar persetujuan yang telah kami
sediakan.
Jika Bapak/Ibu/Sdr/i membutuhkan penjelasan lebih lanjut, maka dapat
menghubung saya di no telp: 08126093740.
Universitas Sumatera Utara
189
Terima kasih saya ucapkan kepada Bapak/Ibu/Sdr/i yang telah bersedia
ikut berpartisipasi pada penelitian ini. Keikutsertaan Bapak/Ibu/Sdr/i dalam
penelitian ini akan menyumbangkan sesuatu yang berguna bagi ilmu
pengetahuan.
Medan, ....................2013
dr Shahrul Rahman, Sp.PD
Universitas Sumatera Utara
190
SURAT PERSETUJUAN SETELAH PENJELASAN
(INFORMED CONSENT)
Saya yang bertanda tangan di bawah ini :
Nama
: ………………………………………………
Alamat
: ………………………………………………
Umur
: …... tahun
Jenis Kelamin : Pria / Wanita
Setelah mempelajari dan mendapat penjelasan yang sejelas-jelasnya
mengenai penelitian yang berjudul : EFEK EKSTRAK BUNCIS (Phaseolus
vulgaris L) TERHADAP KADAR LDL DAN OxLDL SERTA KAITANNYA
DENGAN POLIMORFISME GEN LOX-1 3‟UTR188CT SEBAGAI UPAYA
PREVENTIF RISIKO ATEROSKLEROSIS dan setelah mengetahui dan
menyadari sepenuhnya tentang risiko efek samping yang mungkin akan
terjadi, dengan ini saya menyatakan bahwa saya bersedia mengikuti
prosedur penelitian dengan sadar, sukarela dan tanpa paksaan untuk
menjadi subyek dalam penelitian ini.
Medan, ....................2013
Yang membuat pernyataan
( ........................................... )
Peneliti
dr Shahrul Rahman, Sp.PD
Universitas Sumatera Utara
191
Oneway
Descriptives
Umur (tahun)
N
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
Total
Mean
43.00
38.20
44.43
38.78
40.83
9
10
7
9
35
Std. Deviation
4.555
5.308
4.614
5.167
5.426
Std. Error
1.518
1.679
1.744
1.722
.917
95% Confidence Interval for
Mean
Lower Bound
Upper Bound
39.50
46.50
34.40
42.00
40.16
48.70
34.81
42.75
38.96
42.69
Minimum
36
31
37
30
30
Maximum
50
48
50
46
50
Test of Homogeneity of Variances
Umur (tahun)
Levene
Statistic
.122
df1
df2
3
Sig.
.946
31
ANOVA
Umur (tahun)
Between Groups
Within Groups
Total
Sum of
Squares
240.102
760.870
1000.971
df
3
31
34
Mean Square
80.034
24.544
F
3.261
Sig.
.035
Post Hoc Tests
Multiple Comparisons
Dependent Variable: Umur (tahun)
LSD
(I) Pemberian obat
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
(J) Pemberian obat
Dosis 80 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Mean
Difference
Std. Error
(I-J)
4.800*
2.276
-1.429
2.497
4.222
2.335
-4.800*
2.276
-6.229*
2.441
-.578
2.276
1.429
2.497
6.229*
2.441
5.651*
2.497
-4.222
2.335
.578
2.276
-5.651*
2.497
Sig.
.043
.571
.080
.043
.016
.801
.571
.016
.031
.080
.801
.031
95% Confidence Interval
Lower Bound
Upper Bound
.16
9.44
-6.52
3.66
-.54
8.99
-9.44
-.16
-11.21
-1.25
-5.22
4.06
-3.66
6.52
1.25
11.21
.56
10.74
-8.99
.54
-4.06
5.22
-10.74
-.56
*. The mean difference is significant at the .05 level.
Universitas Sumatera Utara
192
Oneway
Descriptives
KGD
N
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
Total
Mean
87.00
87.70
80.00
86.78
85.74
9
10
7
9
35
Std. Deviation
8.675
8.166
7.616
7.032
8.114
Std. Error
2.892
2.582
2.878
2.344
1.372
95% Confidence Interval for
Mean
Lower Bound
Upper Bound
80.33
93.67
81.86
93.54
72.96
87.04
81.37
92.18
82.96
88.53
Minimum
77
76
73
77
73
Maximum
106
107
94
97
107
Test of Homogeneity of Variances
KGD
Levene
Statistic
.037
df1
df2
3
Sig.
.990
31
ANOVA
KGD
Between Groups
Within Groups
Total
Sum of
Squares
293.030
1945.656
2238.686
df
3
31
34
Mean Square
97.677
62.763
F
1.556
Sig.
.220
Post Hoc Tests
Multiple Comparisons
Dependent Variable: KGD
LSD
(I) Pemberian obat
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
(J) Pemberian obat
Dosis 80 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Mean
Difference
(I-J)
-.700
7.000
.222
.700
7.700
.922
-7.000
-7.700
-6.778
-.222
-.922
6.778
Std. Error
3.640
3.992
3.735
3.640
3.904
3.640
3.992
3.904
3.992
3.735
3.640
3.992
Sig.
.849
.089
.953
.849
.058
.802
.089
.058
.100
.953
.802
.100
95% Confidence Interval
Lower Bound
Upper Bound
-8.12
6.72
-1.14
15.14
-7.39
7.84
-6.72
8.12
-.26
15.66
-6.50
8.35
-15.14
1.14
-15.66
.26
-14.92
1.36
-7.84
7.39
-8.35
6.50
-1.36
14.92
Universitas Sumatera Utara
193
Oneway
Descriptives
Ureum
N
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
Total
9
10
7
9
35
Mean
20.89
20.80
19.43
18.22
19.89
Std. Deviation
5.622
5.594
2.370
4.577
4.788
Std. Error
1.874
1.769
.896
1.526
.809
95% Confidence Interval for
Mean
Lower Bound
Upper Bound
16.57
25.21
16.80
24.80
17.24
21.62
14.70
21.74
18.24
21.53
Minimum
15
13
16
10
10
Maximum
33
29
22
25
33
Test of Homogeneity of Variances
Ureum
Levene
Statistic
1.685
df1
df2
3
Sig.
.191
31
ANOVA
Ureum
Between Groups
Within Groups
Total
Sum of
Squares
43.784
735.759
779.543
df
3
31
34
Mean Square
14.595
23.734
F
.615
Sig.
.611
Post Hoc Tests
Multiple Comparisons
Dependent Variable: Ureum
LSD
(I) Pemberian obat
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
(J) Pemberian obat
Dosis 80 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Mean
Difference
(I-J)
.089
1.460
2.667
-.089
1.371
2.578
-1.460
-1.371
1.206
-2.667
-2.578
-1.206
Std. Error
2.238
2.455
2.297
2.238
2.401
2.238
2.455
2.401
2.455
2.297
2.238
2.455
Sig.
.969
.556
.254
.969
.572
.258
.556
.572
.627
.254
.258
.627
95% Confidence Interval
Lower Bound
Upper Bound
-4.48
4.65
-3.55
6.47
-2.02
7.35
-4.65
4.48
-3.53
6.27
-1.99
7.14
-6.47
3.55
-6.27
3.53
-3.80
6.21
-7.35
2.02
-7.14
1.99
-6.21
3.80
Universitas Sumatera Utara
194
Oneway
Descriptives
Creatinin
N
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
Total
9
10
7
9
35
Mean
.8367
.8040
.7614
.8289
.8103
Std. Deviation
.25725
.26222
.16088
.17934
.21607
Std. Error
.08575
.08292
.06081
.05978
.03652
95% Confidence Interval for
Mean
Lower Bound
Upper Bound
.6389
1.0344
.6164
.9916
.6126
.9102
.6910
.9667
.7361
.8845
Minimum
.59
.52
.60
.59
.52
Maximum
1.41
1.33
1.10
1.08
1.41
Test of Homogeneity of Variances
Creatinin
Levene
Statistic
.867
df1
df2
3
Sig.
.468
31
ANOVA
Creatinin
Between Groups
Within Groups
Total
Sum of
Squares
.026
1.561
1.587
df
3
31
34
Mean Square
.009
.050
F
.175
Sig.
.912
Post Hoc Tests
Multiple Comparisons
Dependent Variable: Creatinin
LSD
(I) Pemberian obat
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
(J) Pemberian obat
Dosis 80 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Mean
Difference
(I-J)
.03267
.07524
.00778
-.03267
.04257
-.02489
-.07524
-.04257
-.06746
-.00778
.02489
.06746
Std. Error
.10310
.11308
.10578
.10310
.11058
.10310
.11308
.11058
.11308
.10578
.10310
.11308
Sig.
.753
.511
.942
.753
.703
.811
.511
.703
.555
.942
.811
.555
95% Confidence Interval
Lower Bound
Upper Bound
-.1776
.2429
-.1554
.3059
-.2080
.2235
-.2429
.1776
-.1830
.2681
-.2352
.1854
-.3059
.1554
-.2681
.1830
-.2981
.1632
-.2235
.2080
-.1854
.2352
-.1632
.2981
Universitas Sumatera Utara
195
Oneway
Descriptives
Ox-LDL (pre)
N
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
Total
Mean
238.33
144.50
251.14
225.44
210.77
9
10
7
9
35
Std. Deviation
67.067
94.151
58.453
76.054
85.171
Std. Error
22.356
29.773
22.093
25.351
14.397
95% Confidence Interval for
Mean
Lower Bound
Upper Bound
186.78
289.89
77.15
211.85
197.08
305.20
166.98
283.91
181.51
240.03
Minimum
134
44
127
77
44
Maximum
317
277
297
302
317
Test of Homogeneity of Variances
Ox-LDL (pre)
Levene
Statistic
2.444
df1
df2
3
Sig.
.083
31
ANOVA
Ox-LDL (pre)
Between Groups
Within Groups
Total
Sum of
Squares
64102.592
182539.6
246642.2
df
3
31
34
Mean Square
21367.531
5888.374
F
3.629
Sig.
.024
Post Hoc Tests
Multiple Comparisons
Dependent Variable: Ox-LDL (pre)
LSD
(I) Pemberian obat
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
(J) Pemberian obat
Dosis 80 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Mean
Difference
Std. Error
(I-J)
93.833*
35.258
-12.810
38.671
12.889
36.174
-93.833*
35.258
-106.643*
37.816
-80.944*
35.258
12.810
38.671
106.643*
37.816
25.698
38.671
-12.889
36.174
80.944*
35.258
-25.698
38.671
Sig.
.012
.743
.724
.012
.008
.029
.743
.008
.511
.724
.029
.511
95% Confidence Interval
Lower Bound
Upper Bound
21.92
165.74
-91.68
66.06
-60.89
86.67
-165.74
-21.92
-183.77
-29.52
-152.85
-9.04
-66.06
91.68
29.52
183.77
-53.17
104.57
-86.67
60.89
9.04
152.85
-104.57
53.17
*. The mean difference is significant at the .05 level.
Universitas Sumatera Utara
196
Oneway
Descriptives
Ox-LDL (post)
N
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
Total
Mean
265.78
261.50
302.43
283.00
276.31
9
10
7
9
35
Std. Deviation
72.475
54.062
20.582
53.796
54.877
Std. Error
24.158
17.096
7.779
17.932
9.276
95% Confidence Interval for
Mean
Lower Bound
Upper Bound
210.07
321.49
222.83
300.17
283.39
321.46
241.65
324.35
257.46
295.17
Minimum
84
163
276
184
84
Maximum
329
313
331
338
338
Test of Homogeneity of Variances
Ox-LDL (post)
Levene
Statistic
1.244
df1
df2
3
Sig.
.311
31
ANOVA
Ox-LDL (post)
Between Groups
Within Groups
Total
Sum of
Squares
8369.773
94019.770
102389.5
df
3
31
34
Mean Square
2789.924
3032.896
F
.920
Sig.
.443
Post Hoc Tests
Multiple Comparisons
Dependent Variable: Ox-LDL (post)
LSD
(I) Pemberian obat
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Placebo
(J) Pemberian obat
Dosis 80 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 120 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Placebo
Dosis 40 mg
Dosis 80 mg
Dosis 120 mg
Mean
Difference
(I-J)
4.278
-36.651
-17.222
-4.278
-40.929
-21.500
36.651
40.929
19.429
17.222
21.500
-19.429
Std. Error
25.304
27.754
25.961
25.304
27.140
25.304
27.754
27.140
27.754
25.961
25.304
27.754
Sig.
.867
.196
.512
.867
.142
.402
.196
.142
.489
.512
.402
.489
95% Confidence Interval
Lower Bound
Upper Bound
-47.33
55.89
-93.25
19.95
-70.17
35.73
-55.89
47.33
-96.28
14.42
-73.11
30.11
-19.95
93.25
-14.42
96.28
-37.18
76.03
-35.73
70.17
-30.11
73.11
-76.03
37.18
Universitas Sumatera Utara
197
NPar Tests (Dosis 40 mg)
One-Sample Kolmogorov-Smirnov Test
a,b
Normal Parameters
Mean
Std. Deviation
N
Kolesterol
(pre)
Kolesterol
(post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida
(pre)
Trigiserida
(post)
Ox-LDL (pre)
Ox-LDL (post)
Most Extreme Differences
Absolute
Positive
Negative
Kolmogorov-Smirnov Z
Asymp. Sig. (2-tailed)
9
214.11
64.449
.237
.110
-.237
.712
.691
9
228.11
48.048
.266
.266
-.190
.799
.547
9
9
9
9
137.67
137.44
45.67
47.44
48.003
28.076
18.527
14.492
.145
.121
.177
.166
.120
.121
.177
.166
-.145
-.106
-.132
-.102
.434
.364
.532
.499
.992
.999
.940
.965
9
158.11
95.241
.189
.189
-.124
.567
.904
9
163.44
86.986
.190
.179
-.190
.571
.900
9
9
238.33
265.78
67.067
72.475
.188
.287
.168
.192
-.188
-.287
.564
.860
.909
.450
a. Test distribution is Normal.
b. Calculated from data.
T-Test
Paired Samples Statistics
Pair
1
Pair
2
Pair
3
Pair
4
Pair
5
Kolesterol (pre)
Kolesterol (post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida (pre)
Trigiserida (post)
Ox-LDL (pre)
Ox-LDL (post)
Mean
214.11
228.11
137.67
137.44
45.67
47.44
158.11
163.44
238.33
265.78
N
Std. Error
Mean
21.483
16.016
16.001
9.359
6.176
4.831
31.747
28.995
22.356
24.158
Std. Deviation
64.449
48.048
48.003
28.076
18.527
14.492
95.241
86.986
67.067
72.475
9
9
9
9
9
9
9
9
9
9
Paired Samples Correlations
N
Pair 1
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) &
Kolesterol (post)
LDL (pre) & LDL (post)
HDL (pre) & HDL (post)
Trigiserida (pre) &
Trigiserida (post)
Ox-LDL (pre) & Ox-LDL
(post)
Correlation
Sig.
9
.852
.004
9
9
.956
.881
.000
.002
9
.947
.000
9
.499
.172
Paired Samples Test
Paired Differences
Std. Deviation
Std. Error
Mean
-14.000
34.406
11.469
-40.447
12.447
-1.221
8
.257
.222
-1.778
22.703
8.969
7.568
2.990
-17.229
-8.672
17.674
5.116
.029
-.595
8
8
.977
.569
-5.333
30.859
10.286
-29.053
18.387
-.518
8
.618
-27.444
70.009
23.336
-81.258
26.369
-1.176
8
.273
Mean
Pair 1
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) Kolesterol (post)
LDL (pre) - LDL (post)
HDL (pre) - HDL (post)
Trigiserida (pre) Trigiserida (post)
Ox-LDL (pre) - Ox-LDL
(post)
95% Confidence
Interval of the
Difference
Lower
Upper
t
df
Sig. (2-tailed)
Universitas Sumatera Utara
198
NPar Tests (Dosis 80 mg)
One-Sample Kolmogorov-Smirnov Test
a,b
Normal Parameters
Mean
Std. Deviation
N
Kolesterol
(pre)
Kolesterol
(post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida
(pre)
Trigiserida
(post)
Ox-LDL (pre)
Ox-LDL (post)
Most Extreme Differences
Absolute
Positive
Negative
Kolmogorov-Smirnov Z
Asymp. Sig. (2-tailed)
10
197.60
36.078
.143
.137
-.143
.454
.986
10
199.20
36.411
.142
.142
-.132
.450
.988
10
10
10
10
125.00
121.20
48.10
45.70
33.400
20.682
10.408
10.853
.183
.147
.154
.198
.115
.106
.154
.198
-.183
-.147
-.143
-.162
.579
.465
.486
.627
.891
.982
.972
.827
10
123.60
71.071
.206
.206
-.167
.653
.787
10
146.60
110.368
.356
.356
-.222
1.126
.158
10
10
144.50
261.50
94.151
54.062
.199
.313
.199
.170
-.178
-.313
.628
.989
.825
.282
a. Test distribution is Normal.
b. Calculated from data.
T-Test
Paired Samples Statistics
Pair
1
Pair
2
Pair
3
Pair
4
Pair
5
Kolesterol (pre)
Kolesterol (post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida (pre)
Trigiserida (post)
Ox-LDL (pre)
Ox-LDL (post)
Mean
197.60
199.20
125.00
121.20
48.10
45.70
123.60
146.60
144.50
261.50
N
Std. Deviation
36.078
36.411
33.400
20.682
10.408
10.853
71.071
110.368
94.151
54.062
10
10
10
10
10
10
10
10
10
10
Std. Error
Mean
11.409
11.514
10.562
6.540
3.291
3.432
22.475
34.902
29.773
17.096
Paired Samples Correlations
N
Pair 1
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) &
Kolesterol (post)
LDL (pre) & LDL (post)
HDL (pre) & HDL (post)
Trigiserida (pre) &
Trigiserida (post)
Ox-LDL (pre) & Ox-LDL
(post)
Correlation
Sig.
10
.726
.017
10
10
.787
.938
.007
.000
10
.712
.021
10
-.340
.336
Paired Samples Test
Paired Differences
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) Kolesterol (post)
LDL (pre) - LDL (post)
HDL (pre) - HDL (post)
Trigiserida (pre) Trigiserida (post)
Ox-LDL (pre) - Ox-LDL
(post)
t
Std. Deviation
Std. Error
Mean
-1.600
26.825
8.483
-20.790
17.590
-.189
9
.855
3.800
2.400
21.369
3.777
6.757
1.194
-11.486
-.302
19.086
5.102
.562
2.009
9
9
.588
.075
-23.000
77.866
24.623
-78.702
32.702
-.934
9
.375
-117.000
123.500
39.054
-205.346
-28.654
-2.996
9
.015
Mean
Pair 1
95% Confidence
Interval of the
Difference
Lower
Upper
df
Sig. (2-tailed)
Universitas Sumatera Utara
199
NPar Tests (Dosis 120 mg)
One-Sample Kolmogorov-Smirnov Test
a,b
Normal Parameters
Mean
Std. Deviation
N
Kolesterol
(pre)
Kolesterol
(post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida
(pre)
Trigiserida
(post)
Ox-LDL (pre)
Ox-LDL (post)
Most Extreme Differences
Absolute
Positive
Negative
Kolmogorov-Smirnov Z
Asymp. Sig. (2-tailed)
7
214.71
50.255
.229
.179
-.229
.605
.858
7
215.71
31.952
.221
.164
-.221
.585
.883
7
7
7
7
139.57
138.57
50.29
47.71
45.048
33.040
6.157
4.821
.163
.162
.216
.181
.145
.162
.216
.168
-.163
-.094
-.155
-.181
.431
.428
.572
.478
.992
.993
.899
.976
7
125.86
42.807
.248
.162
-.248
.657
.781
7
144.43
47.233
.126
.126
-.096
.334
1.000
7
7
251.14
302.43
58.453
20.582
.288
.212
.216
.212
-.288
-.187
.763
.562
.606
.910
a. Test distribution is Normal.
b. Calculated from data.
T-Test
Paired Samples Statistics
Pair
1
Pair
2
Pair
3
Pair
4
Pair
5
Kolesterol (pre)
Kolesterol (post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida (pre)
Trigiserida (post)
Ox-LDL (pre)
Ox-LDL (post)
Mean
214.71
215.71
139.57
138.57
50.29
47.71
125.86
144.43
251.14
302.43
N
7
7
7
7
7
7
7
7
7
7
Std. Deviation
50.255
31.952
45.048
33.040
6.157
4.821
42.807
47.233
58.453
20.582
Std. Error
Mean
18.995
12.077
17.026
12.488
2.327
1.822
16.180
17.852
22.093
7.779
Paired Samples Correlations
N
Pair 1
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) &
Kolesterol (post)
LDL (pre) & LDL (post)
HDL (pre) & HDL (post)
Trigiserida (pre) &
Trigiserida (post)
Ox-LDL (pre) & Ox-LDL
(post)
Correlation
Sig.
7
.870
.011
7
7
.852
.857
.015
.014
7
.878
.009
7
.330
.469
Paired Samples Test
Paired Differences
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) Kolesterol (post)
LDL (pre) - LDL (post)
HDL (pre) - HDL (post)
Trigiserida (pre) Trigiserida (post)
Ox-LDL (pre) - Ox-LDL
(post)
t
Std. Deviation
Std. Error
Mean
-1.000
27.459
10.379
-26.395
24.395
-.096
6
.926
1.000
2.571
24.194
3.207
9.144
1.212
-21.375
-.395
23.375
5.538
.109
2.121
6
6
.916
.078
-18.571
22.619
8.549
-39.491
2.348
-2.172
6
.073
-51.286
55.190
20.860
-102.328
-.244
-2.459
6
.049
Mean
Pair 1
95% Confidence
Interval of the
Difference
Lower
Upper
df
Sig. (2-tailed)
Universitas Sumatera Utara
200
NPar Tests (Plasebo)
One-Sample Kolmogorov-Smirnov Test
a,b
Normal Parameters
Mean
Std. Deviation
N
Kolesterol
(pre)
Kolesterol
(post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida
(pre)
Trigiserida
(post)
Ox-LDL (pre)
Ox-LDL (post)
Most Extreme Differences
Absolute
Positive
Negative
Kolmogorov-Smirnov Z
Asymp. Sig. (2-tailed)
9
187.22
27.833
.199
.199
-.169
.598
.868
9
201.89
26.582
.172
.129
-.172
.517
.952
9
9
9
9
118.44
127.11
43.44
44.78
25.279
24.700
5.790
10.390
.177
.237
.136
.308
.121
.237
.107
.308
-.177
-.222
-.136
-.199
.531
.710
.408
.923
.940
.694
.996
.362
9
125.56
72.236
.236
.236
-.127
.707
.699
9
124.56
68.608
.214
.214
-.197
.642
.805
9
9
225.44
283.00
76.054
53.796
.259
.196
.157
.153
-.259
-.196
.776
.589
.584
.879
a. Test distribution is Normal.
b. Calculated from data.
T-Test
Paired Samples Statistics
Pair
1
Pair
2
Pair
3
Pair
4
Pair
5
Mean
187.22
201.89
118.44
127.11
43.44
44.78
125.56
124.56
225.44
283.00
Kolesterol (pre)
Kolesterol (post)
LDL (pre)
LDL (post)
HDL (pre)
HDL (post)
Trigiserida (pre)
Trigiserida (post)
Ox-LDL (pre)
Ox-LDL (post)
N
Std. Error
Mean
9.278
8.861
8.426
8.233
1.930
3.463
24.079
22.869
25.351
17.932
Std. Deviation
27.833
26.582
25.279
24.700
5.790
10.390
72.236
68.608
76.054
53.796
9
9
9
9
9
9
9
9
9
9
Paired Samples Correlations
N
Pair 1
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) &
Kolesterol (post)
LDL (pre) & LDL (post)
HDL (pre) & HDL (post)
Trigiserida (pre) &
Trigiserida (post)
Ox-LDL (pre) & Ox-LDL
(post)
Correlation
Sig.
9
.432
.245
9
9
.583
.733
.099
.025
9
.943
.000
9
-.268
.486
Paired Samples Test
Paired Differences
Std. Deviation
Std. Error
Mean
-14.667
29.009
9.670
-36.965
7.631
-1.517
8
.168
-8.667
-1.333
22.814
7.297
7.605
2.432
-26.203
-6.943
8.870
4.276
-1.140
-.548
8
8
.287
.599
1.000
24.005
8.002
-17.452
19.452
.125
8
.904
-57.556
104.261
34.754
-137.697
22.586
-1.656
8
.136
Mean
Pair 1
Pair 2
Pair 3
Pair 4
Pair 5
Kolesterol (pre) Kolesterol (post)
LDL (pre) - LDL (post)
HDL (pre) - HDL (post)
Trigiserida (pre) Trigiserida (post)
Ox-LDL (pre) - Ox-LDL
(post)
95% Confidence
Interval of the
Difference
Lower
Upper
t
df
Sig. (2-tailed)
Universitas Sumatera Utara
201
Kebun Buncis di Berastagi
Laboratorium Obat Tradisional Fakultas Farmasi USU
Universitas Sumatera Utara
202
Penyuluhan dan Pengambilan Darah di Rumah Sakit Putri Hijau
Universitas Sumatera Utara
203
Eijkman Institute Jakarta
Universitas Sumatera Utara