KAJIAN IN VITRO PENGGUNAAN KROMIUM (Cr) ORGANIK DALAM RANSUM SAPI PERAH TERHADAP POPULASI MIKROBA RUMEN DAN PRODUKSI GAS TOTAL.
KAJIAN IN VITRO PENGGUNAAN KROMIUM ORGANIK DALAM
RANSUM SAPI PERAH TERHADAP POPULASI MIKROBA RUMEN
DAN PRODUKSI GAS TOTAL
Panji Akbar Wijayadikusumah
ABSTRAK
Penelitian dilaksanakan selama satu bulan, dari bulan Februari sampai
Maret 2013 di Laboratorium Nutrisi Ternak Ruminansia dan Kimia Makanan
Ternak dan di Laboratorium Mikrobiologi dan Penanganan Limbah
Peternakan, Fakultas Peternakan, Universitas Padjadjaran. Penelitian bertujuan
untuk mengetahui pengaruh penggunaan kromium organik dalam ransum sapi
perah terhadap populasi mikroba rumen dan produksi gas total yang dikaji
secara in vitro. Penelitian dilakukan dengan metode eksperimental, rancangan
percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan
empat perlakuan penggunaan kromium organik, yaitu : R1 = 0 ppm, R2 = 1,5
ppm, R3 = 3 ppm dan R4 = 4,5 ppm, masing-masing perlakuan diulang
sebanyak lima kali. Data yang diperoleh dianalisis dengan menggunakan
Analisis Sidik Ragam, kemudian u ntuk mengetahui pebedaan antar perlakuan
dilakukan uji lanjut menggunakan, Uji Jarak Berganda Duncan. Peubah yang
diukur adalah jumlah populasi mikroba rumen dan produksi gas total. Hasil
penelitian menunjukkan bahwa penggunaan kromium organik dalam ransum
sapi perah berpengaruh terhadap jumlah total populasi bakteri dan produksi gas
total, tetapi tidak berpengaruh terhadap jumlah populasi protozoa. Penggunaan
4,5 ppm kromium organik dalam ransum menghasilkan nilai rataan total
populasi bakteri, populasi protozoa dan produksi gas tertinggi.
Kata Kunci : Kromium organik, ransum sapi perah, populasi mikroba
rumen, produksi gas total
IN VITRO STUDY OF ORGANIC CHROMIUM IN DAIRY CATTLE
RATION ON RUMEN MICROBES POPULATION AND TOTAL GAS
PRODUCTION
Panji Akbar Wijayadikusumah
ABSTRACT
This research was conducted for one month, from February to March
2013 at Laboratory of Animal Nutrition of Ruminant and Feed Chemistry and
at Laboratory Microbiology and Animal Husbandry Waste Management,
Faculty of Animal Husbandry, Padjadjaran University. The study aimed to
determine the effect of the use of organic chromium in the ration of Dairy
Cattle on rumen microbial population and total gas production which was
studied in In Vitro. Research was carried out by the experimental method,
experimental design used was completely randomized design (CRD) with four
treatments using organic chromium, namely: R1 = 0 ppm, R2 = 1.5 ppm, R3 =
R4 = 3 ppm and 4.5 ppm and each treatment was repeated five times. The data
obtained were analyzed by using the Fingerprint Analysis of Variety and then
to determine the average difference among treatments, it was done the tested
further with Duncan Multiple Range Test. The variables measured were the
number of rumen microbial population and total gas production. The results
were showed that the use of organic chromium in dairy cow rations affected
the total bacterial population and total gas production, but had no effect on
protozoa population. The use of 4.5 ppm chromium in the ration of organic
produced average value of the total population of bacteria, protozoa population
and the highest gas production.
Keywords: Chromium organic, dairy cattle rations, rumen microbial
populations, total gas production
RANSUM SAPI PERAH TERHADAP POPULASI MIKROBA RUMEN
DAN PRODUKSI GAS TOTAL
Panji Akbar Wijayadikusumah
ABSTRAK
Penelitian dilaksanakan selama satu bulan, dari bulan Februari sampai
Maret 2013 di Laboratorium Nutrisi Ternak Ruminansia dan Kimia Makanan
Ternak dan di Laboratorium Mikrobiologi dan Penanganan Limbah
Peternakan, Fakultas Peternakan, Universitas Padjadjaran. Penelitian bertujuan
untuk mengetahui pengaruh penggunaan kromium organik dalam ransum sapi
perah terhadap populasi mikroba rumen dan produksi gas total yang dikaji
secara in vitro. Penelitian dilakukan dengan metode eksperimental, rancangan
percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan
empat perlakuan penggunaan kromium organik, yaitu : R1 = 0 ppm, R2 = 1,5
ppm, R3 = 3 ppm dan R4 = 4,5 ppm, masing-masing perlakuan diulang
sebanyak lima kali. Data yang diperoleh dianalisis dengan menggunakan
Analisis Sidik Ragam, kemudian u ntuk mengetahui pebedaan antar perlakuan
dilakukan uji lanjut menggunakan, Uji Jarak Berganda Duncan. Peubah yang
diukur adalah jumlah populasi mikroba rumen dan produksi gas total. Hasil
penelitian menunjukkan bahwa penggunaan kromium organik dalam ransum
sapi perah berpengaruh terhadap jumlah total populasi bakteri dan produksi gas
total, tetapi tidak berpengaruh terhadap jumlah populasi protozoa. Penggunaan
4,5 ppm kromium organik dalam ransum menghasilkan nilai rataan total
populasi bakteri, populasi protozoa dan produksi gas tertinggi.
Kata Kunci : Kromium organik, ransum sapi perah, populasi mikroba
rumen, produksi gas total
IN VITRO STUDY OF ORGANIC CHROMIUM IN DAIRY CATTLE
RATION ON RUMEN MICROBES POPULATION AND TOTAL GAS
PRODUCTION
Panji Akbar Wijayadikusumah
ABSTRACT
This research was conducted for one month, from February to March
2013 at Laboratory of Animal Nutrition of Ruminant and Feed Chemistry and
at Laboratory Microbiology and Animal Husbandry Waste Management,
Faculty of Animal Husbandry, Padjadjaran University. The study aimed to
determine the effect of the use of organic chromium in the ration of Dairy
Cattle on rumen microbial population and total gas production which was
studied in In Vitro. Research was carried out by the experimental method,
experimental design used was completely randomized design (CRD) with four
treatments using organic chromium, namely: R1 = 0 ppm, R2 = 1.5 ppm, R3 =
R4 = 3 ppm and 4.5 ppm and each treatment was repeated five times. The data
obtained were analyzed by using the Fingerprint Analysis of Variety and then
to determine the average difference among treatments, it was done the tested
further with Duncan Multiple Range Test. The variables measured were the
number of rumen microbial population and total gas production. The results
were showed that the use of organic chromium in dairy cow rations affected
the total bacterial population and total gas production, but had no effect on
protozoa population. The use of 4.5 ppm chromium in the ration of organic
produced average value of the total population of bacteria, protozoa population
and the highest gas production.
Keywords: Chromium organic, dairy cattle rations, rumen microbial
populations, total gas production