PENGARUH SARI BUAH MENGKUDU Morinda citrifolia L. TERHADAP RESPON FISIOLOGIS IKAN GURAMI Osphronemus gouramy PADA KEPADATAN YANG BERBEDA = THE INFLUENCE OF SQUEEZED NONI Morinda citrifolia L. ON PHYSIOLOGICAL RESPONSE OF GOURAMY Osphronemus gouramy AT DIF
RINGKASAN
ALIF FAIZATUL ROSYIDAH. Pengaruh Sari Buah Mengkudu Morinda
citrifolia L. terhadap Respon Fisiologis Ikan Gurami Osphronemus gouramy
pada Kepadatan yang Berbeda. Dosen Pembimbing Akhmad Taufiq Mukti
dan Serta Laksmi Sulmartiwi.Ikan gurami merupakan ikan air tawar yang memiliki gizi tinggi dan nilai
ekonomis penting serta banyak digemari oleh masyarakat Indonesia. Pada kondisi
budidaya, ikan sering menjadi subyek terhadap perubahan atau stresor seperti
handling , kepadatan, transportasi, dan perubahan kualitas air. Padat tebar dalam
budidaya yang berbeda selain dapat menyebabkan kompetisi ruang gerak dan
perebutan oksigen terlarut pada ikan, juga dapat menyebabkan ikan mengalami
stres. Buah mengkudu dapat dimanfaatkan dengan membuat sari buah mengkudu.
Penelitian ini bertujuan untuk mengetahui pengaruh pemberian sari buah
mengkudu terhadap respon fisiologi ikan gurami pada kepadatan yang berbeda.
Penelitian ini menggunakan metode eksperimental dengan rancangan acak
lengkap faktorial yang terdiri dari dua faktor yaitu konsentrasi sari buah
mengkudu (0%, 1% dan 2%) dan kepadatan ikan gurami (10, 20 dan 30 ekor).
Masing-masing perlakuan diulang tiga kali. Parameter yang diamati pada
penelitian ini adalah glukosa darah, sel darah merah (eritrosit), sel darah putih
(leukosit) dan buka tutup operculum (tachiventilasi) ikan gurami. Analisis data
menggunakan Analisis Varian (ANAVA) dan dilanjutkan Uji Jarak Berganda
Duncan
Hasil penelitian ini menunjukkan bahwa perendaman sari buah mengkudu
pada kepadatan yang berbeda berpengaruh nyata (p<0,05) terhadap glukosa darah,
sel darah merah (eritrosit), sel darah putih (leukosit) dan buka tutup operculum
(tachiventilasi) ikan gurami. Perlakuan optimal yaitu penggunaan konsentrasi 2%
sari buah mengkudu dengan kepadatan 3 ekor/literv
SUMMARY
ALIF FAIZATUL ROSYIDAH. The Influence of Squeezed Noni Morinda
citrifolia L. on Physiological Response of Gouramy Osphronemus gouramy at
Different Density. Advisor Akhmad Taufiq Mukti and Laksmi Sulmartiwi.Gouramy is a freshwater fish that has high nutrition and important
economic value and much favored by the people of Indonesia. In aquaculture
conditions, fish are often subjected to changes or stressors such as handling,
density, transportation, and changes in water quality. Solid stocking in a different
cultivation can additionally lead to competition of space and seizure of dissolved
oxygen in fish, can also cause fish to experience stress. Noni fruit can be utilized
by making juice mengkudu.This study aims to determine the effect of noni freshly squeezed on
physiology response of gouramy fish at different densities. This research used
experimental method with complete factorial randomized design consisting of two
factors it is concentration of Noni juice (0%, 1% and 2%) and gouramy density
(10, 20 and 30). Each is repeated three times. Parameters observed in this study
were blood glucose, red blood cells (erythrocytes), white blood cells (leukocytes)
and open the lid of the operculum (tachiventilation) of gouramy. Data analysis
using Analysis of Variant (ANAVA) and continued Duncan Multiple Range Test.
The results of this study showed that immersion of noni juice extract at
different density significantly (p <0.05) on blood glucose, red blood cells
(erythrocytes), white blood cells (leukocytes) and open the lid of the gouramy
operculum (tachiventilation). Optimal treatment is the use of 2% concentration of
squeezed noni with density 3 fish/liter.vi
Kusriningrum, RS., 2008. Buku Ajar Perancangan Percobaan. Dani Abadi.
Surabaya, hal 43-51.
Lagler, K. F., J. E. Bardach., R. R. Miller., D. R. M. Passino. 1977. Ichtiology.
John Wiley & Sons, Inc. United State of America. 191-371
Mahasri, G., A.S Mubarak., dan M.A Alamsjah. 2007. Bahan Ajar Manajemen
Kualitas Air Faultas Perikanan dan Kelautan. Universitas Airlangga. 125hal.Moyle, P. B, dan J. J. Cech, Jr. 2004. Fishes An Introduction to Ichthyology.
Prentice Hall, Upper Saddle River. 15p.
Nayak B. S., G. N. Isitor., A. Maxwell., V. Bhogadi., D. D. Ramdath. 2008.
Wound Healing Activity of Morinda citrifolia Fruit Juice on Diabetes Induced Rats. J. Wound Care. 16(2):83-86.
Nayak, S. dan Mengi, S., 2010. Immunostimulant Activity of Noni (Morinda
citrifolia ) on T and B Lymphocytes. Pharmaceutical Biology, 48: 724 –731.Neiffer, D.L, and Stamper, M.A. 2009. Fish sedation, Anesthesia, Analgesia, and
Euthanasia Consideration, Methods, and Types of Drugs. ILAR Journal50(4): 343-60.
Nelson, C. 2006. Species Profiles for Pacific Island Agroforestry
www.traditionaltree.org
“Morinda citrifolia”.
Nuryati, S., Suparman dan Hadiroseyani. 2008. Penggunaan Ekstrak Daun
Paci-paci (Leucas sp.) untuk Pencegahan Penyakit Mikotik pada IkanGurame (Osphronemus gouramy Lac.). Jurnal Akuakultur Indonesia. 7(2): 205-212 hlm
Pichavant, K., V. Maxime, M.T. Theubault, H. Ollivier, J. P. Garnier, B.
Bousquet, M. Diouris, G. Nonnotte. 2002. Effects of Hypoxia and Subsequent Recovery on Turbot Scophthalmus maximus. Hormonal Changes and Anaerobic Metabolism. Marine Ecology Proggram Series. Vol. 225: 275-285.
Porchas, M. M., L. R. M. Cordova and R. R Enriquez. 2009. Cortisol and glucosa:
Reliable Indicators of Fish Stress. Pan - American Journal of AquaticSciences, 4(2):158 –178
Rahardjo, M.F., Sjafei,D. S., Affandi. R., & Sulistiono. 2011. Ikhtiologi. Jakarta.
Lubuk Agung. 396 hal.
Randall, D.J. and S.F. Perry. 1992. Catecholamines. In Fish Physiology. Edited by
D.J Randall and W.S Hoar. Academic Press, New York. 12:255-300.
Rethinam, P; Sivaraman, K. 2007. Noni (Morinda citrifolia L.) The Miracle Fruit
- A Holistic Review. International Journal of noni Research. 2 (1). pp.5-6.Robert, T.R. 1992. Systematic Revision of the Southeast Asian Anabantoid Fish
Genus Osphronemus, with Description of Two New Spesies. IchthyolExplor, Freshwater 2(4):351-360.
Ross LG, Ross B. 2008. Anasthetic and Sedative Techniques for Aquatic
Animals. Blackwell: United State of America. Pp.10-15.
Saanin, H. 1984. Taksonomi dan Kunci Identifikasi Ikan. Binacipta, Jakarta. p:
33-44.Sanjaya, Y. 2011. Pengaruh Konsentrasi Larutan Daun Bandotan (Ageratum
conyzoides) Terhadap Tingkat Kelulusan dan Buka Tutup Operkulum Benih Ikan Koi (Cyprinus Carpio) dalam Transportasi Sistem Tertutup. Skripsi.Fakultas Perikanan dan Kelautan. Universitas Airlangga. Surabaya. v,24 hal.
Schreck CB, Moyle PB. 1990. Methods for Fish Biology. American Fisheries
Society. Maryland. USA. 553 p.
Setiawati, M., T. I. Winarno, M. A. Suprayudi, I. Mokoginta, W. Manalu. 2007.
Mineral Besi Sebagai Peningkat Vitalitas Ikan Kerapu Bebek (Cromileptes altivelis) Saat Kondisi Stres Hipoksia. Jurnal Ilmu Pertanian Indonesia. 12 (1): 8-14.
Sitawati, R. I. 2002. Interaksi Antara Deterjen, Tingkat Stres dan Uji Tantang
Bakteri Aeromonas hydriphila pada Ikan Mas (Cyprinus carpio Lin.Skripsi.Program Studi Budidaya Perairan Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor. Bogor. 53 hal.
Standar Nasional Indonesia. 2009. Standar Nasional Indonesia Pakan Buatan
Untuk Ikan Gurami (Osphronemus gouramy Lac.). Jakarta. BadanStandardisasi Nasional. Steward, M. 1991. Animal Physiology. Thomson Litho Ltd., London. 486 p.
Takashima, dan Hibiya. 1995. An Atlas of Fish Normal and Pathologucal
Features. Japan : Kodansha. Pp.147.
Tantarpale, V.T., S.H Rathod and Sunita Kapil. 2012. Temperature Stress on
Opercular Beats and Respiratory Rate of Freshwater Fish Channa punctatus . International Journal of Scientific and Research Publications, 2: 1-5.
Tavarez-Dias, M., Sandrim. E.F.S., Moraes, F. R and Carneiro, P. C. F. 2001.
Physiological Responses of “Tambaqui” Colossoma macropomum (Characidae) to Acute Stress. Boletim do Instituto de Pesca, 27 (1): 43-48.
Utami, E.T., R. Fitrianti, Mahriani, S. Fajariyah. 2002. Efek Kondisi Hiperglemik
terhadap Struktur Ovarium dan Siklus Estrus Mencit Mus musculus L.Jurnal Ilmu Dasar 10 (2): 219-224.
Vail, B. 2004. Diabetes mellitus. Current Diagnosis and Treatment in Family
Medicine. USA. McGraw-Hill Companies.
Wang, M.Y., West, B.J., Jensen, C.J., Nowicki, D., Chen, S., Palu, A.K., dan
Anderson, G., 2002. Morinda citrifolia (Noni): A literature review and recent advances in Noni research, Acta Pharmacologica Sinica, 23 (13): 1127-1141.
Wedemeyer GA. 1996. Physiology of Fish in Intensive Culture System. Chapman
and Hall. 115 Fifth Avenue New York. 89: 1-17Wedemeyer, G. A. and D. J. McLeay. 1981. Methods for Determining the
Tolerance of Fishes to Environtmental Stressor. Didalam : Pickering, A. D.
(editor). Stress and Fish. Academic Press, Inc. London. hal . 247-276.
Wendelaar, B.S.E. 1997. The Stress Response in Fish. Physiological Review.
77: 591-625.
Zahl IH, Kiessling A, Samuelsen OB, Hansen MK. 2011. Anaesthesia of
Atlantic Halibut (Hippoglossus hippoglossus) Effect of Pre-AnaestheticSedation and Importance of Body Weight and Water Temperature. Aquaculture. 42:1235
- –1245
Zonneveld N., E. A Huisman and J. H. Boon. 1991. Prinsip-prinsip Budidaya
Ikan. Gramedia Pustaka Utama. Jakarta. hal 164-298
LAMPIRAN Lampiran 1. Data glukosa darah ikan gurami
34
42
40 A1B2
41
42
3 A1B1
2
1
32 Jam ke-72 Perlakuan Ulangan
33
46 A1B3
34 A3B3
35
34
35 A3B2
37
38
39 A3B1
40
38
44
46
43
32
32
31
30
32 A3B3
31
31
33 A3B2
31
30 A3B1
45
33
36
35 A2B3
32
34
40 A2B2
38
36
48 A2B1
42 A2B3
Jam ke-6 Perlakuan Ulangan
1
74 A2B1
58
62
59 A2B3
57
61
66 A2B2
60
61
71
55
72
69 A1B3
70
67
54 A1B2
58
56
3 A1B1
2
56 A3B1
54
46 A2B2
49
43
44
47 A2B1
48
49
46 A1B3
48
47
42 A1B2
46
61 A3B2
3 A1B1
2
1
54 Jam ke- 24 Perlakuan Ulangan
52
53
58 A3B3
55
52
41 Lampiran 1. (lanjutan) Jam ke-144 Perlakuan Ulangan
1
34
29
29
31 A3B3
30
30
33 A3B2
30
32
34 A3B1
32
31
32 A2B3
33
2
37 A2B2
36
35
44 A2B1
45
44
45 A1B3
43
40
30 A1B2
36
35
3 A1B1
31
Lampiran 2. Data Sel Darah Merah (Eritrosit) Ikan Gurami
Jam ke-6 Perlakuan Ulangan
1
2
3 A1B1 3210000 3250000 3185000 A1B2 3170000 3290000 3300000 A1B3 3290000 3280000 3360000 A2B1 3350000 3290000 3280000 A2B2 3250000 3290000 3310000 A2B3 3290000 3250000 3270000 A3B1 3200000 3260000 3195000 A3B2 3180000 3170000 3200000 A3B3 3100000 3195000 3180000 Jam ke-24 Perlakuan Ulangan
1
2
3 A1B1 2640000 2690000 2720000 A1B2 2670000 2710000 2740000 A1B3 2800000 2770000 2750000 A2B1 2710000 2740000 2780000 A2B2 2690000 2740000 2670000 A2B3 2740000 2640000 2710000 A3B1 2560000 2470000 2590000 A3B2 2490000 2540000 2525000 A3B3 2390000 2320000 2250000 Jam ke-72 Perlakuan Ulangan
1
2
3 A1B1 2310000 2380000 2390000 A1B2 2500000 2450000 2430000 A1B3 2520000 2550000 2510000 A2B1 2400000 2430000 2425000 A2B2 2390000 2400000 2310000 A2B3 2350000 2310000 2390000 A3B1 2330000 2300000 2390000 A3B2 2310000 2280000 2350000 A3B3 2210000 2270000 2190000 Lampiran 2. (lanjutan) Jam ke-144 Perlakuan Ulangan
1
2
3 A1B1 2260000 2210000 2300000 A1B2 2220000 2360000 2270000 A1B3 2390000 2400000 2360000 A2B1 2370000 2390000 2340000 A2B2 2350000 2370000 2340000 A2B3 2350000 2380000 2310000 A3B1 2300000 2350000 2340000 A3B2 2300000 2290000 2230000 A3B3 2190000 2180000 2250000
Lampiran 3. Data Sel Darah Putih (Leukosit) Ikan Gurami
Jam ke-6 Perlakuan Ulangan
1
2
3 A1B1 478000 471500 465000 A1B2 488000 493000 498500 A1B3 509500 500000 499500 A2B1 485000 481000 492000 A2B2 457000 445500 455000 A2B3 441500 445000 442000 A3B1 439000 445000 439000 A3B2 445000 442000 430000 A3B3 410500 407000 413500 Jam ke-24 Perlakuan Ulangan
1
2
3 A1B1 435000 442500 440500 A1B2 482000 480500 481000 A1B3 490000 491000 492500 A2B1 490000 485500 475000 A2B2 450500 445000 448000 A2B3 439500 450000 435500 A3B1 427500 423000 429000 A3B2 425000 423500 421500 A3B3 410000 405000 406500 Jam ke-72 Perlakuan Ulangan
1
2
3 A1B1 427000 435000 429000 A1B2 480500 473500 475500 A1B3 488000 476000 481000 A2B1 467500 471000 476000 A2B2 445000 441000 440500 A2B3 436000 435500 432000 A3B1 423000 430500 424000 A3B2 415000 423000 419000 A3B3 405000 409500 402500 Lampiran 3. (lanjutan) Jam ke-144 Perlakuan Ulangan
1
2
3 A1B1 420000 425000 421000 A1B2 449500 445000 455000 A1B3 451500 459500 449000 A2B1 441500 455000 443000 A2B2 440500 439500 441000 A2B3 431000 433000 430500 A3B1 418500 420000 425000 A3B2 412500 413000 407000 A3B3 401000 407000 405500
Lampiran 4. Data Tachiventilasi Ikan Gurami
Jam ke-24 Ulangan Perlakuan
1
2
3 A1B1 232 242 239 A1B2 238 241 243 A1B3 250 249 247 A2B1 230 227 229 A2B2 234 228 229 A2B3 229 230 235 A3B1 216 209 212 A3B2 224 218 221 A3B3 224 221 232 Jam ke-72 Ulangan Perlakuan
1
2
3 A1B1 237 239 233 A1B2 232 238 241 A1B3 243 241 239 A2B1 216 220 213 A2B2 224 228 221 A2B3 230 232 229 A3B1 201 212 205 A3B2 208 212 210 A3B3 211 213 216 Jam ke-144 Ulangan Perlakuan
1
2
3 A1B1 218 224 220 A1B2 230 234 229 A1B3 235 237 241 A2B1 212 205 211 A2B2 209 213 211 A2B3 218 214 212 A3B1 192 195 203 A3B2 200 197 201 A3B3 210 207 209
Lampiran 5. Data tingkat kelangsungan hidup ikan gurami
60
60
70
60
65 A3B3
55
65
80 A3B2
90
80
60 A3B1
60
50
65 A2B3
Perlakuan Ulangan
1
80 A2B2
70
80
60 A2B1
50
50
55 A1B3
50
65
90 A1B2
80
90
3 A1B1
2
55
Lampiran 6. Data kualitas air selama pemeliharaan ikan gurami
Perlak uan Ulan gan
Jam ke-6 Jam ke-24 Jam ke-144 Ph suhu Do amon ia Ph Suhu Do Amo nia Ph Suhu Do amon ia A1B1
1 7,42 29,1 4,66 7 28,3 5,25 7,30 28,2 4,57 2 7,48 29,7 5,05 7,24 28,3 5,77 7,58 28,7 6,16 3 7,62 28,6 5,68 7,28 27,7 6,12 7,53 28,3 5,69 A1B2 1 7,3 28,8 3,34 6,69 28,4 2,53 7,46 28,2 5,91 2 6,84 28,7 2,18 6,44 27,8 2,79 7,64 28,2 5,32 3 7,08 28,9 2,24 6,47 28,2 2,33 7,53 28,1 5,69 A1B3 1 6,66 29,4 2,07 6,23 28,6 2,26 7,45 28,8 5,65 2 6,52 29,6 2,12 6,65 28,7 2,89 7,26 28,8 4,28 3 6,57 29,1 2,07 5,42 28,5 2,12 7,41 28,7 5,48 A2B1 1 7,32 29,1 5,46 7,08 28,4 4,99 7,34 28,9 4,63 2 7,53
29 5,42 6,96 28,2 5,25 7,41 28,8 5,13
3 7 28,8 3,07 6,67 28,6 2,65 7,47 28,5 4,92 A2B2 1 7,31 29 3,31 6,48 28,3 2,62 7,43 28,2 4,22 2 6,94
29 3 6,39 28,4 2,45 7,40 28,3 5,2 3 7,09 28,8 2,5 6,56 28,3 2,86 7,38 28,9 5,60 A2B3 1 6,56 29,3 2,1 6,52 29,1 2,39 7,48 28,7 5,35 2 6,74 29,1 2,15 6,39 28,4 2,14 7,51 28,4 5,75
3 6,66 28,8 2,11 5,62 28,1 2,09 7,61 28,4 6,11 A3B1 1 7,09 29,2 3,79 6,87 28,7 3,74 7,15 29 2,68 2 7,06 29,5 3,08 6,74 28,7 2,76 7,34 28,9 4,59 3 7,18 28,7 4,51 6,98 27,9 4,74 7,29 28,4 5,43 A3B2 1 7 28,9 2,23 6,60 28,2 2,30 7,48 28 4,26 2 6,74 29,4 2,08 6,44 29,6 2,13 7,34 28,9 4,69 3 7 28,6 3,84 6,64 27,9 2,49 7,04 28,5 3,11 A3B3 1 6,70 29,1 2,1 6,05 28,4 2,08 7,39 28,2 5,61 2 6,87 29,2 2,75 6,45 28,4 2,19 7,39 28,3 5,81 3 7 29,3 2,26 6,63 28,4 2,74 7,58 28,5 4,85
Lampiran 7. Hasil analisis varian (ANAVA) glukosa darah ikan gurami
Corrected Model 1008.741
52.00
74.00 Test of Homogeneity of Variances Levene Statistic df1 df2 Sig.
1.218
8 18 .343
Tests of Between-Subjects Effects
Source Type III Sum of Squares df Mean Square F Sig.
Partial Eta Squared
a
52.00
8 126.093 20.026 .000 .899 Intercept 97800.926 1 97800.926 1.553E4 .000 .999 perlakuan_A 523.185
2 261.593 41.547 .000 .822 perlakuan_B 47.185 2 23.593 3.747 .044 .294 perlakuan_A * perlakuan_B
438.370 4 109.593 17.406 .000 .795 Error
113.333 18 6.296 Total
98923.000
27 Corrected Total 1122.074
26
54.00 Total 27 60.1852 6.56938 1.26428 57.5864 62.7839
Jam ke-6
Descriptives
N Mean Std.
71.00
Deviation Std. Error 95% Confidence Interval for
Mean Minimum Maximum Lower Bound Upper Bound
A1B1 3 56.0000 2.00000 1.15470 51.0317 60.9683
54.00
58.00 A1B2 3 68.6667 1.52753 .88192 64.8721 72.4612
67.00
70.00 A1B3 3 72.3333 1.52753 .88192 68.5388 76.1279
74.00 A2B1 3 62.3333 3.21455 1.85592 54.3479 70.3187
52.00
60.00
66.00 A2B2 3 59.0000 2.00000 1.15470 54.0317 63.9683
57.00
61.00 A2B3 3 58.6667 3.05505 1.76383 51.0775 66.2558
56.00
62.00 A3B1 3 56.6667 3.78594 2.18581 47.2619 66.0715
54.00
61.00 A3B2 3 55.0000 3.00000 1.73205 47.5476 62.4524
58.00 A3B3 3 53.0000 1.00000 .57735 50.5159 55.4841 Lampiran 7. (lanjutan) ANOVA Sum of Squares Df Mean Square F Sig.
Between Groups 1008.741 8 126.093 20.026 .000 Within Groups 113.333 18 6.296 Total 1122.074
26 Subset for alpha = 0.05 perlakuan kombinasi N
1
2
3
4 A3B3 3 53.0000 A3B2 3 55.0000 55.0000 A1B1 3 56.0000 56.0000 A3B1 3 56.6667 56.6667 A2B3 3 58.6667 58.6667 A2B2 3 59.0000 59.0000 A2B1 3 62.3333 A1B2
3 68.6667
A1B3
3 72.3333 Sig.
.116 .094 .106 .090
Jam ke-24
Descriptives
95% Confidence Interval for Mean Std.
N Mean Deviation Std. Error Lower Bound Upper Bound Minimum Maximum A1B1 3 45.6667 3.51188 2.02759 36.9427 54.3907
42.00
49.00 A1B2 3 47.0000 1.00000 .57735 44.5159 49.4841
46.00
48.00 A1B3 3 48.0000 1.00000 .57735 45.5159 50.4841
47.00
49.00 A2B1 3 44.3333 1.52753 .88192 40.5388 48.1279
43.00
46.00 A2B2 3 42.0000 1.00000 .57735 39.5159 44.4841
41.00
43.00 A2B3 3 39.0000 1.00000 .57735 36.5159 41.4841
38.00
40.00 A3B1 3 36.6667 1.52753 .88192 32.8721 40.4612
35.00
38.00 A3B2 3 34.3333 .57735 .33333 32.8991 35.7676
34.00
35.00 A3B3 3 33.0000 1.00000 .57735 30.5159 35.4841
32.00
34.00 Total 27 41.1111 5.52848 1.06396 38.9241 43.2981
32.00
49.00 Lampiran 7. (lanjutan)
Tests of Between-Subjects Effects
Type III Sum Partial Eta Source of Squares df Mean Square F Sig. Squared
a
Corrected Model 750.000 8 93.750 37.780 .000 .944 Intercept 45633.333 1 45633.333 1.839E4 .000 .999 perlakuan_A 678.222
2 339.111 136.657 .000 .938 perlakuan_B 22.222 2 11.111 4.478 .026 .332 perlakuan_A *
49.556 4 12.389 4.993 .007 .526 perlakuan_B Error
44.667 18 2.481 Total
46428.000
27 Corrected Total 794.667
26 Test of Homogeneity of Variances Levene Statistic df1 df2 Sig.
1.644
8 18 .181
ANOVA Sum of Squares df Mean Square F Sig.
Between Groups 750.000 8 93.750 37.780 .000 Within Groups
44.667 18 2.481 Total
794.667
26 Subset for alpha = 0.05 perlakuan kombinasi N
1
2
3
4
5
6 A3B3 3 33.0000 A3B2 3 34.3333 34.3333 A3B1 3 36.6667 36.6667 A2B3 3 39.0000 A2B2 3 42.0000 A2B1 3 44.3333 44.3333 A1B1
3 45.6667 45.6667
A1B2
3 47.0000 47.0000
A1B3
3 48.0000 Sig.
.314 .086 .086 .086 .064 .102 Lampiran 7. (lanjutan) Jam ke-72
Descriptives
95% Confidence Interval for Mean Std.
N Mean Deviation Std. Error Lower Bound Upper Bound Minimum Maximum A1B1 3 41.0000 1.00000 .57735 38.5159 43.4841
40.00
42.00 A1B2 3 44.0000 2.00000 1.15470 39.0317 48.9683
42.00
46.00 A1B3 3 46.3333 1.52753 .88192 42.5388 50.1279
45.00
48.00 A2B1 3 38.0000 2.00000 1.15470 33.0317 42.9683
36.00
40.00 A2B2 3 33.6667 1.52753 .88192 29.8721 37.4612
32.00
35.00 A2B3 3 33.0000 3.00000 1.73205 25.5476 40.4524
30.00
36.00 A3B1 3 32.0000 1.00000 .57735 29.5159 34.4841
31.00
33.00 A3B2 3 31.3333 .57735 .33333 29.8991 32.7676
31.00
32.00 A3B3 3 31.0000 1.00000 .57735 28.5159 33.4841
30.00
32.00 Total 27 36.7037 5.77671 1.11173 34.4185 38.9889
30.00
48.00 Tests of Between-Subjects Effects Type III Sum
Partial Eta Source of Squares df Mean Square F Sig. Squared
a
Corrected Model 817.630 8 102.204 36.793 .000 .942
Intercept 36373.370 1 36373.370 1.309E4 .000 .999 perlakuan_A
728.963 2 364.481 131.213 .000 .936 perlakuan_B 2.074 2 1.037 .373 .694 .040 perlakuan_A *
86.593 4 21.648 7.793 .001 .634 perlakuan_B Error
50.000 18 2.778 Total
37241.000
27 Corrected Total 867.630
26 Test of Homogeneity of Variances Levene Statistic df1 df2 Sig.
.826
8 18 .591
ANOVA Sum of Squares df Mean Square F Sig.
Between Groups 817.630 8 102.204 36.793 .000 Within Groups
50.000 18 2.778 Total
867.630
26
Lampiran 7. (lanjutan)
Subset for alpha = 0.05 perlakuan kombinasi N
1
2
3
4 A3B3 3 31.0000 A3B2 3 31.3333 A3B1 3 32.0000 A2B3 3 33.0000 A2B2 3 33.6667 A2B1 3 38.0000 A1B1 3 41.0000 A1B2
3 44.0000
A1B3
3 46.3333 Sig.
.093 1.000 1.000 .104
Jam ke-144
Descriptives
95% Confidence Interval for Mean Std.
N Mean Deviation Std. Error Lower Bound Upper Bound Minimum Maximum A1B1 3 33.6667 3.21455 1.85592 25.6813 41.6521
30.00
36.00 A1B2 3 42.6667 2.51661 1.45297 36.4151 48.9183
40.00
45.00 A1B3 3 44.3333 .57735 .33333 42.8991 45.7676
44.00
45.00 A2B1 3 36.0000 1.00000 .57735 33.5159 38.4841
35.00
37.00 A2B2 3 33.0000 1.00000 .57735 30.5159 35.4841
32.00
34.00 A2B3 3 32.3333 1.52753 .88192 28.5388 36.1279
31.00
34.00 A3B1 3 31.6667 1.52753 .88192 27.8721 35.4612
30.00
33.00 A3B2 3 30.3333 .57735 .33333 28.8991 31.7676
30.00
31.00 A3B3 3 29.6667 1.15470 .66667 26.7982 32.5351
29.00
31.00 Total 27 34.8519 5.23820 1.00809 32.7797 36.9240
29.00
45.00 Lampiran 7. (lanjutan)
Tests of Between-Subjects Effects
Type III Sum Partial Eta Source of Squares df Mean Square F Sig. Squared
a
Corrected Model 662.741 8 82.843 29.431 .000 .929
Intercept 32795.593 1 32795.593 1.165E4 .000 .998 perlakuan_A
436.074 2 218.037 77.461 .000 .896 perlakuan_B 15.630 2 7.815 2.776 .089 .236 perlakuan_A *
211.037 4 52.759 18.743 .000 .806 perlakuan_B Error
50.667 18 2.815 Total
33509.000
27 Corrected Total 713.407
26 Test of Homogeneity of Variances Levene Statistic df1 df2 Sig.
2.477
8 18 .052
ANOVA Sum of Squares df Mean Square F Sig.
Between Groups 662.741 8 82.843 29.431 .000 Within Groups
50.667 18 2.815 Total
713.407
26 Subset for alpha = 0.05 perlakuan kombinasi N
1
2
3
4
5 A3B3 3 29.6667 A3B2 3 30.3333 30.3333 A3B1 3 31.6667 31.6667 31.6667 A2B3 3 32.3333 32.3333 32.3333 A2B2 3 33.0000 33.0000 33.0000 A1B1 3 33.6667 33.6667 A2B1 3 36.0000 A1B2
3 42.6667
A1B3
3 44.3333 Sig.
.089 .089 .196 .051 .239
Lampiran 8. Hasil analisis varian (ANAVA) sel darah merah (eritrosit) ikan gurami
Jam ke-6
Descriptives
95% Confidence Interval for Mean N Mean Std. Deviation Std. Error Lower Bound Upper Bound Minimum Maximum
A1B1 3 3.2150E6 32787.19262 1.89297E4 3.1336E6 3.2964E6
3.18E6
3.25E6 A1B2 3 3.2533E6 72341.78138 4.17665E4 3.0736E6 3.4330E6
3.17E6
3.30E6 A1B3 3 3.3100E6 43588.98944 2.51661E4 3.2017E6 3.4183E6
3.28E6
3.36E6 A2B1 3 3.3067E6 37859.38897 2.18581E4 3.2126E6 3.4007E6
3.28E6
3.35E6 A2B2 3 3.2833E6 30550.50463 1.76383E4 3.2074E6 3.3592E6
3.25E6
3.31E6 A2B3 3 3.2700E6 20000.00000 1.15470E4 3.2203E6 3.3197E6
3.25E6
3.29E6 A3B1 3 3.2183E6 36170.89069 2.08833E4 3.1285E6 3.3082E6
3.20E6
3.26E6 A3B2 3 3.1833E6 15275.25232 8.81917E3 3.1454E6 3.2213E6
3.17E6
3.20E6 A3B3 3 3.1583E6 51071.84482 2.94863E4 3.0315E6 3.2852E6
3.10E6
3.20E6 Total 27 3.2443E6 61888.60501 1.19105E4 3.2198E6 3.2687E6
3.10E6
3.36E6
Tests of Between-Subjects Effects
Type III Sum of Partial Eta
Source Squares df Mean Square F Sig. Squared
a
Corrected Model 6.934E10 8 8.667E9 5.157 .002 .696 Intercept 2.842E14 1 2.842E14 1.691E5 .000 1.000 perlakuan_A 4.811E10
2 2.406E10 14.315 .000 .614 perlakuan_B 2.463E8 2 1.231E8 .073 .930 .008 perlakuan_A *
2.098E10 4 5.244E9 3.120 .041 .409 perlakuan_B Error
3.025E10 18 1.681E9 Total
2.843E14
27 Corrected Total 9.959E10
26 Test of Homogeneity of Variances Levene Statistic df1 df2 Sig.
2.304
8 18 .067 Lampiran 8. (lanjutan) ANOVA Sum of Squares df Mean Square F Sig.
Between Groups 6.934E10 8 8.667E9 5.157 .002 Within Groups
3.025E10 18 1.681E9 Total
9.959E10
26 Subset for alpha = 0.05 perlakuan kombinasi N
1
2
3
4 A3B3 3 3.1583E6 A3B2 3 3.1833E6 3.1833E6 A1B1 3 3.2150E6 3.2150E6 3.2150E6 A3B1 3 3.2183E6 3.2183E6 3.2183E6 A1B2 3 3.2533E6 3.2533E6 3.2533E6 A2B3 3 3.2700E6 3.2700E6 A2B2 3 3.2833E6 3.2833E6 A2B1
3 3.3067E6
A1B3
3 3.3100E6 Sig.
.116 .069 .081 .144
Jam ke-24
Descriptives
95% Confidence Interval for Mean N Mean Std. Deviation Std. Error Lower Bound Upper Bound Minimum Maximum
A1B1 3 2.6833E6 40414.51884 2.33333E4 2.5829E6 2.7837E6
2.64E6
2.72E6 A1B2 3 2.7067E6 35118.84584 2.02759E4 2.6194E6 2.7939E6
2.67E6
2.74E6 A1B3 3 2.7733E6 25166.11478 1.45297E4 2.7108E6 2.8358E6
2.75E6
2.80E6 A2B1 3 2.7433E6 35118.84584 2.02759E4 2.6561E6 2.8306E6
2.71E6
2.78E6 A2B2 3 2.7000E6 36055.51275 2.08167E4 2.6104E6 2.7896E6
2.67E6
2.74E6 A2B3 3 2.6967E6 51316.01439 2.96273E4 2.5692E6 2.8241E6
2.64E6
2.74E6 A3B1 3 2.5400E6 62449.97998 3.60555E4 2.3849E6 2.6951E6
2.47E6
2.59E6 A3B2 3 2.5183E6 25658.00720 1.48137E4 2.4546E6 2.5821E6
2.49E6
2.54E6 A3B3 3 2.3200E6 70000.00000 4.04145E4 2.1461E6 2.4939E6
2.25E6
2.39E6 Total 27 2.6313E6 1.44193E5 2.77499E4 2.5743E6 2.6883E6
2.25E6
2.80E6 Lampiran 8. (lanjutan)
Tests of Between-Subjects Effects
Type III Sum Partial Eta
Source of Squares Df Mean Square F Sig. Squared
a
Corrected Model 5.043E11 8 6.304E10 31.303 .000 .933 Intercept 1.869E14 1 1.869E14 9.283E4 .000 1.000 perlakuan_A 3.990E11
2 1.995E11 99.054 .000 .917 perlakuan_B 1.706E10 2 8.529E9 4.235 .031 .320 perlakuan_A *
8.830E10 4 2.208E10 10.962 .000 .709 perlakuan_B Error
3.625E10 18 2.014E9 Total
1.875E14
27 Corrected Total 5.406E11
26 Test of Homogeneity of Variances Levene Statistic df1 df2 Sig.
.750
8 18 .649
ANOVA Sum of Squares df Mean Square F Sig.
Between Groups 5.043E11 8 6.304E10 31.303 .000 Within Groups
3.625E10 18 2.014E9 Total
5.406E11
26 Subset for alpha = 0.05 perlakuan kombinasi N
1
2
3
4 A3B3 3 2.3200E6 A3B2 3 2.5183E6 A3B1 3 2.5400E6 A1B1 3 2.6833E6 A2B3 3 2.6967E6 2.6967E6 A2B2 3 2.7000E6 2.7000E6 A1B2 3 2.7067E6 2.7067E6 A2B1 3 2.7433E6 2.7433E6 A1B3
3 2.7733E6 Sig.
1.000 .562 .157 .074 Lampiran 8. (lanjutan) Jam ke-72
Descriptives
95% Confidence Interval for Mean Std.
N Mean Deviation Std. Error Lower Bound Upper Bound Minimum Maximum A1B1 3 2.3600E6 43588.98944 2.51661E4 2.2517E6 2.4683E6
2.31E6
2.39E6 A1B2 3 2.4600E6 36055.51275 2.08167E4 2.3704E6 2.5496E6
2.43E6
2.50E6 A1B3 3 2.5267E6 20816.65999 1.20185E4 2.4750E6 2.5784E6
2.51E6
2.55E6 A2B1 3 2.4183E6 16072.75127 9.27961E3 2.3784E6 2.4583E6
2.40E6
2.43E6 A2B2 3 2.3667E6 49328.82862 2.84800E4 2.2441E6 2.4892E6
2.31E6
2.40E6 A2B3 3 2.3500E6 40000.00000 2.30940E4 2.2506E6 2.4494E6
2.31E6
2.39E6 A3B1 3 2.3400E6 45825.75695 2.64575E4 2.2262E6 2.4538E6
2.30E6
2.39E6 A3B2 3 2.3133E6 35118.84584 2.02759E4 2.2261E6 2.4006E6
2.28E6
2.35E6 A3B3 3 2.2233E6 41633.31999 2.40370E4 2.1199E6 2.3268E6
2.19E6
2.27E6 Total 27 2.3731E6 89669.78039 1.72570E4 2.3377E6 2.4086E6
2.19E6
2.55E6
Tests of Between-Subjects Effects
Type III Sum of Partial Eta
Source Squares df Mean Square F Sig. Squared
a
Corrected Model 1.831E11 8 2.288E10 15.853 .000 .876 Intercept 1.521E14 1 1.521E14 1.053E5 .000 1.000 perlakuan_A 1.108E11
2 5.541E10 38.383 .000 .810 perlakuan_B 8.019E8 2 4.009E8 .278 .761 .030 perlakuan_A *
7.146E10 4 1.786E10 12.376 .000 .733 perlakuan_B Error
2.598E10 18 1.444E9 Total
1.523E14
27 Corrected Total 2.091E11
26 Test of Homogeneity of Variances Levene Statistic df1 df2 Sig.
.784
8 18 .623 Lampiran 8. (lanjutan) ANOVA Sum of Squares Df Mean Square F Sig.
Between Groups 1.831E11 8 2.288E10 15.853 .000 Within Groups
2.598E10 18 1.444E9 Total
2.091E11
26 Subset for alpha = 0.05 perlakuan kombinasi N
1
2
3
4
5 A3B3 3 2.2233E6 A3B2 3 2.3133E6 A3B1 3 2.3400E6 A2B3 3 2.3500E6 2.3500E6 A1B1 3 2.3600E6 2.3600E6 A2B2 3 2.3667E6 2.3667E6 A2B1 3 2.4183E6 2.4183E6 A1B2 3 2.4600E6 A1B3
3 2.5267E6 Sig.
1.000 .138 .057 .196 1.000
Jam ke-144
Descriptives
95% Confidence Interval for Mean
N Mean Std. Deviation Std. Error Lower Bound Upper Bound Minimum Maximum A1B1 3 2.2567E6 45092.49753 2.60342E4 2.1447E6 2.3687E6
2.21E6
2.30E6 A1B2 3 2.2833E6 70945.98885 4.09607E4 2.1071E6 2.4596E6
2.22E6
2.36E6 A1B3 3 2.3833E6 20816.65999 1.20185E4 2.3316E6 2.4350E6
2.36E6
2.40E6 A2B1 3 2.3667E6 25166.11478 1.45297E4 2.3042E6 2.4292E6
2.34E6
2.39E6 A2B2 3 2.3533E6 15275.25232 8.81917E3 2.3154E6 2.3913E6
2.34E6
2.37E6 A2B3 3 2.3467E6 35118.84584 2.02759E4 2.2594E6 2.4339E6
2.31E6
2.38E6 A3B1 3 2.3300E6 26457.51311 1.52753E4 2.2643E6 2.3957E6
2.30E6
2.35E6 A3B2 3 2.2733E6 37859.38897 2.18581E4 2.1793E6 2.3674E6
2.23E6
2.30E6 A3B3 3 2.2067E6 37859.38897 2.18581E4 2.1126E6 2.3007E6
2.18E6
2.25E6 Total 27 2.3111E6 64945.73672 1.24988E4 2.2854E6 2.3368E6
2.18E6
2.40E6
Tests of Between-Subjects Effects Type III Sum Partial Eta
Source of Squares df Mean Square F Sig. Squared
a
Corrected Model 8.333E10 8 1.042E10 7.120 .000 .760 Intercept 1.442E14 1 1.442E14 9.858E4 .000 1.000 perlakuan_A 3.309E10
2 1.654E10 11.309 .001 .557 perlakuan_B 9.556E8 2 4.778E8 .327 .726 .035 perlakuan_A *
4.929E10 4 1.232E10 8.423 .001 .652 perlakuan_B Error
2.633E10 18 1.463E9 Total
1.443E14
27 Corrected Total 1.097E11
26 Test of Homogeneity of Variances Levene Statistic df1 df2 Sig.
1.347
8 18 .284
ANOVA Sum of Squares df Mean Square F Sig.
Between Groups 8.333E10 8 1.042E10 7.120 .000 Within Groups
2.633E10 18 1.463E9 Total
1.097E11
26 Subset for alpha = 0.05 perlakuan kombinasi N
1
2
3
4
5 A3B3 3 2.2067E6 A1B1 3 2.2567E6 2.2567E6 A3B2 3 2.2733E6 2.2733E6 2.2733E6 A1B2 3 2.2833E6 2.2833E6 2.2833E6 A3B1 3 2.3300E6 2.3300E6 2.3300E6 A2B3 3 2.3467E6 2.3467E6 A2B2 3 2.3533E6 2.3533E6 A2B1
3 2.3667E6
A1B3
3 2.3833E6 Sig.
.057 .430 .102 .053 .140
Lampiran 9. Hasil analisis varian (ANAVA) sel darah putih (leukosit) ikan gurami
Jam ke-6
Descriptives
95% Confidence Interval for Mean
N Mean Std. Deviation Std. Error Lower Bound Upper Bound Minimum Maximum A1B1 3 4.7150E5 6500.00000 3.75278E3 455353.1049 487646.8951
4.65E5
4.78E5 A1B2 3 4.9317E5 5251.98375 3.03223E3 480120.0158 506213.3176
4.88E5
4.98E5 A1B3 3 5.0300E5 5634.71383 3.25320E3 489002.5949 516997.4051
5.00E5
5.10E5 A2B1 3 4.8600E5 5567.76436 3.21455E3 472168.9066 499831.0934
4.81E5
4.92E5 A2B2 3 4.5250E5 6144.10286 3.54730E3 437237.2024 467762.7976
4.46E5
4.57E5 A2B3 3 4.4283E5 1892.96945 1.09291E3 438130.9365 447535.7301
4.42E5
4.45E5 A3B1 3 4.4100E5 3464.10162 2.00000E3 432394.6945 449605.3055
4.39E5
4.45E5 A3B2 3 4.3900E5 7937.25393 4.58258E3 419282.7682 458717.2318
4.30E5
4.45E5 A3B3 3 4.1033E5 3253.20355 1.87824E3 402251.9277 418414.7390
4.07E5
4.14E5 Total 27 4.5993E5 29515.21892 5.68021E3 448250.0942 471601.7577
4.07E5
5.10E5
Tests of Between-Subjects Effects
Type III Sum Partial Eta
Source of Squares Df Mean Square F Sig. Squared
a
Corrected Model 2.213E10 8 2.766E9 95.943 .000 .977 Intercept 5.711E12 1 5.711E12 1.981E5 .000 1.000 perlakuan_A 1.573E10
2 7.864E9 272.726 .000 .968 perlakuan_B 9.319E8 2 4.660E8 16.160 .000 .642 perlakuan_A *
5.472E9 4 1.368E9 47.443 .000 .913 perlakuan_B Error
5.190E8 18 2.883E7 Total
5.734E12
27 Corrected Total 2.265E10
26 Test of Homogeneity of Variances Levene Statistic df1 df2 Sig.
.998
8 18 .470
Lampiran 9. (lanjutan)
ANOVA Sum of Squares Df Mean Square F Sig.
Between Groups 2.213E10 8 2.766E9 95.943 .000 Within Groups
5.190E8 18 2.883E7 Total
2.265E10
26 Subset for alpha = 0.05 perlakuan kombinasi N
1
2
3
4
5
6 A3B3 3 4.1033E5 A3B2 3 4.3900E5 A3B1 3 4.4100E5 A2B3 3 4.4283E5 A2B2 3 4.5250E5 A1B1 3 4.7150E5 A2B1
3 4.8600E5
A1B2
3 4.9317E5
A1B3
3 5.0300E5 Sig.
1.000 .419 1.000 1.000 .119 1.000
Jam ke-24
Descriptives
95% Confidence Interval for Mean
N Mean Std. Deviation Std. Error Lower Bound Upper Bound Minimum Maximum A1B1 3 4.3933E5 3883.72673 2.24227E3 429685.6213 448981.0454
4.35E5
4.42E5 A1B2 3 4.8117E5 763.76262 4.40959E2 479269.3751 483063.9582
4.80E5
4.82E5 A1B3 3 4.9117E5 1258.30574 7.26483E2 488040.8619 494292.4714
4.90E5
4.92E5 A2B1 3 4.8350E5 7697.40216 4.44410E3 464378.5930 502621.4070
4.75E5
4.90E5 A2B2 3 4.4783E5 2753.78527 1.58990E3 440992.5515 454674.1152
4.45E5
4.50E5 A2B3 3 4.4167E5 7488.88065 4.32371E3 423063.2558 460270.0775
4.36E5
4.50E5 A3B1 3 4.2650E5 3122.49900 1.80278E3 418743.2825 434256.7175
4.23E5
4.29E5 A3B2 3 4.2333E5 1755.94229 1.01379E3 418971.3309 427695.3358
4.22E5
4.25E5 A3B3 3 4.0717E5 2565.80072 1.48137E3 400792.8643 413540.4690
4.05E5
4.10E5 Total 27 4.4907E5 28768.87464 5.53657E3 437693.4863 460454.6618
4.05E5
4.92E5
Lampiran 9. (lanjutan)
Tests of Between-Subjects Effects
Type III Sum of Partial Eta
Source Squares df Mean Square F Sig. Squared
a
Corrected Model 2.120E10 8 2.650E9 149.449 .000 .985 Intercept 5.445E12 1 5.445E12 3.071E5 .000 1.000 perlakuan_A 1.296E10
2 6.479E9 365.385 .000 .976 perlakuan_B 8.274E7 2 4.137E7 2.333 .126 .206 perlakuan_A *
8.159E9 4 2.040E9 115.040 .000 .962 perlakuan_B Error
3.192E8 18 1.773E7 Total
5.467E12
27 Corrected Total 2.152E10
26 Test of Homogeneity of Variances Levene Statistic df1 df2 Sig.
3.199
8 18 .019
ANOVA Sum of Squares Df Mean Square F Sig.
Between Groups 2.120E10 8 2.650E9 149.449 .000 Within Groups
3.192E8 18 1.773E7 Total
2.152E10
26 Subset for alpha = 0.05 perlakuan kombinasi N
1
2
3
4