Uji Anti Hiperkolesterolemia Daun Pirdot (Saurauia vulcani K.) pada Mencit (Mus musculus L.) Jantan yang Diberi Diet Kuning Telur Puyuh
LAMPIRAN
Lampiran 1. Komposisi Kuning Telur Puyuh
Informasi Rinci Komposisi Kandungan Nutrisi/Gizi Pada Telur Puyuh :
Berat Telur Puyuh yang diteliti (Food Weight)
100 g
Bagian Telur yang dapat dikonsumsi (Bdd / Food Edible) 100 %
Kandungan Energi
168 kkal
Kandungan Protein
12,3 g
Kandungan Lemak
12,7 g
Kandungan Karbohidrat
1,2 g
Kandungan Kalsium
64 mg
Kandungan Fosfor
226 mg
Kandungan Zat Besi
3,65 mg
Kandungan Vitamin A
543 IU
Kandungan Vitamin B12
1,58 mg
Kandungan Vitamin E
1,08 mg
Kandungan Kolin
263,4 mg
Sumber Informasi Gizi : Berbagai publikasi Kementerian Kesehatan
Republik Indonesia
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Lampiran 2. Skrining fitokimia ektrak etanol daun pirdot (Saurauia vulcani .K)
(Sitorus, 2015)
No
Skrining
Simplisia
Ekstrak Etanol
1.
Alkanoid
-
-
2.
Flavonoid
+
+
3.
Glikosida
+
+
4.
Saponin
+
+
5.
Antraquinonglikosida
-
-
6.
Tanin
+
+
7.
Terpenoid/ steroid
+
+
Ket : Ada (+)
Tidak ada (-)
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Lampiran 3. Data Berat Badan mencit yang diberi diet kuning telur dan ekstrak
daun pirdot selama 30 hari.
a. Berat Badan Hari ke- 0
Perlakuan
KO
K1
P1
P2
P3
U1
26
26
25
26
25
U2
28
35
28
25
27
Ulangan
U3
U4
26
32
29
26
26
28
29
29
26
25
U5
25
25
29
25
28
Rata
Rata
STDEV
26.6
29.4
28
25.6
26.2
1.341641
4.159327
1.732051
0.547723
1.30384
Rata
Rata
STDEV
31.2
29.6
33.8
32.8
29.4
1.30384
5.59464
1.30384
1.48324
2.073644
Rata
Rata
STDEV
34.6
32.6
30.2
31
26.8
2.302173
4.827007
1.48324
1
1.30384
Rata
Rata
STDEV
36.6
32.4
28.2
30.4
26.2
3.847077
4.827007
1.923538
0.547723
2.167948
b. Berat Badan Hari ke- 10
Perlakuan
KO
K1
P1
P2
P3
U1
32
27
32
32
27
U2
33
26
33
31
29
Ulangan
U3
U4
30
34
35
33
31
30
37
35
35
28
U5
31
24
34
33
32
c. Berat Badan Hari ke- 20
Perlakuan
KO
K1
P1
P2
P3
U1
37
29
28
30
25
U2
36
36
30
30
26
Ulangan
U3
U4
35
35
31
32
28
31
37
32
32
27
U5
34
26
30
31
28
d. Berat Badan Hari ke- 30
Perlakuan
KO
K1
P1
P2
P3
U1
40
30
26
31
23
U2
37
35
27
30
25
Ulangan
U3
U4
38
36
29
30
28
30
36
31
31
27
U5
38
25
28
30
28
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Lampiran 4. Data Kadar Kolesterol Total mencit setela pemberian diet kung telur
dan ekstrak daun pirdot selama 30 hari.
a. Kadar Kolesterol Total Hari ke-0
Ulangan
Perlakuan
U1
U2
U3
U4
KO
K1
P1
P2
P3
124
112
105
109
116
115
119
102
117
105
105
109
113
123
121
176
157
107
110
125
b. Kadar Kolesterol Total Hari ke-10
Ulangan
Perlakuan
U1
U2
U3
U4
KO
K1
P1
P2
P3
140
117
172
210
210
134
122
193
160
193
191
167
189
225
213
156
158
176
206
281
c. Kadar Kolesterol Total Hari ke-20
Ulangan
Perlakuan
U1
U2
U3
U4
KO
K1
P1
P2
P3
134
127
150
196
132
140
279
146
146
143
107
145
165
206
171
207
158
143
157
245
d. Kadar Kolesterol Total Hari ke-10
Ulangan
Perlakuan
U1
U2
U3
U4
KO
K1
P1
P2
P3
Ket :
129
149
140
123
125
KO
K1
P1
P2
P3
115
224
143
117
140
105
222
158
182
146
154
192
132
146
224
U5
119
126
132
100
115
U5
136
157
199
159
167
U5
124
221
176
125
171
U5
147
239
155
110
150
Rata
Rata
STDEV
127.8
124.6
111.8
111.8
116.4
27.83343
19.26915
11.98749
8.700575
7.536577
Rata
Rata
STDEV
151.4
144.2
185.8
192
212.8
23.76552
22.94995
11.43241
30.5041
42.27529
Rata
Rata
STDEV
142.4
186
156
166
172.4
38.21387
62.88879
14.01785
34.13942
44.0772
Rata
Rata
STDEV
130
205.2
145.6
135.6
157
20.71232
35.74493
10.78425
29.24551
38.63936
: Kontrol Negatif
: Kontrol Positif
: Ekstrak Daun Pirdot 100 mg/kgBB
: Ekstrak Daun Pirdot 200 mg/kgBB
: Ekstrak Daun Pirdot 300 mg/kgBB
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Lampiran 5. Data Kadar Trigliserida, HDL, dan LDL Serum Darah Mencit
Setelah Pemberian Diet Kuning Telur Dan Ekstrak Daun Pirdot
Selama 30 Hari.
a. Trigliserida
Perlakuan
KO
K1
P1
P2
P3
U1
77
175
164
167
148
U2
118
102
150
81
112
Ulangan
U3
87
193
112
179
116
U4
144
134
107
76
105
U5
97
56
194
112
227
Rata Rata
104.6
132
145.4
123
141.6
STDEV
26.7451
55.3399
36.4664
47.8696
50.5104
U2
59
128
87
50
75
Ulangan
U3
70
82
77
64
75
U4
80
87
65
40
59
U5
60
108
91
48
89
Rata Rata
66.8
105.4
77.2
49.8
77
STDEV
8.58487
20.489
11.8406
8.78635
12
U2
32
76
26
51
43
Ulangan
U3
18
101
57
82
48
U4
45
78
46
91
144
U5
68
120
25
40
16
Rata Rata
42.4
93.4
39
61.4
52
STDEV
18.7697
18.0776
13.6198
23.4798
54.0971
b. HDL
Perlakuan
KO
K1
P1
P2
P3
U1
65
122
66
47
87
c. LDL
Perlakuan
KO
K1
P1
P2
P3
Ket :
KO
K1
P1
P2
P3
U1
49
92
41
43
9
: Kontrol Negatif
: Kontrol Positif
: Ekstrak Daun Pirdot 100 mg/kgBB
: Ekstrak Daun Pirdot 200 mg/kgBB
: Ekstrak Daun Pirdot 300 mg/kgBB
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Lampiran 6. Analisis Statistik Berat Badan Mencit Setelah Pemberian Diet
Kuning Telur Dan Ekstrak Daun Pirdot Selama 30 Hari.
a. Berat Badan Hari ke-0
Descriptives
berat badan H0
N
Mean
Std.
Std. Error
95% Confidence
Deviation
Minimum Maximum
Interval for Mean
Lower
Upper
Bound
Bound
KO
5 26.6000
1.34164
.60000
24.9341
28.2659
25.00
28.00
K1
5 29.4000
4.15933
1.86011
24.2355
34.5645
25.00
35.00
P1
5 28.0000
1.73205
.77460
25.8494
30.1506
25.00
29.00
P2
5 25.6000
.54772
.24495
24.9199
26.2801
25.00
26.00
P3
5 26.2000
1.30384
.58310
24.5811
27.8189
25.00
28.00
25 27.1600
2.44404
.48881
26.1511
28.1689
25.00
35.00
Total
Test of Homogeneity of Variances
berat badan H0
Levene Statistic
5.123
df1
df2
4
Sig.
20
.005
ANOVA
berat badan H0
Sum of Squares
df
Mean Square
Between Groups
46.960
4
11.740
Within Groups
96.400
20
4.820
143.360
24
Total
F
2.436
Sig.
.081
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b. Berat badan Hari ke-10
Descriptives
berat badan H10
N
Mean
Std.
Std. Error
95% Confidence
Deviation
Minimum
Maximum
Interval for Mean
Lower
Upper
Bound
Bound
KO
5 31.2000
1.30384
.58310
29.5811
32.8189
30.00
33.00
K1
5 29.6000
5.59464
2.50200
22.6533
36.5467
24.00
37.00
P1
5 33.8000
1.30384
.58310
32.1811
35.4189
32.00
35.00
P2
5 32.8000
1.48324
.66332
30.9583
34.6417
31.00
35.00
P3
5 29.4000
2.07364
.92736
26.8252
31.9748
27.00
32.00
25 31.3600
3.16070
.63214
30.0553
32.6647
24.00
37.00
Total
Test of Homogeneity of Variances
berat badan H10
Levene Statistic
df1
11.074
df2
4
Sig.
20
.000
ANOVA
berat badan H10
Sum of Squares
Between Groups
df
Mean Square
74.960
4
18.740
Within Groups
164.800
20
8.240
Total
239.760
24
F
Sig.
2.274
.097
c. Berat Badan Hari ke-20
Descriptives
berat badan H20
N
Mean
Std.
Std. Error
Deviation
95% Confidence
Minimum
Maximum
Interval for Mean
Lower
Upper
Bound
Bound
KO
5
34.6000
2.30217
1.02956
31.7415
37.4585
31.00
37.00
K1
5
32.6000
4.82701
2.15870
26.6065
38.5935
26.00
37.00
P1
5
30.2000
1.48324
.66332
28.3583
32.0417
28.00
32.00
P2
5
31.0000
1.00000
.44721
29.7583
32.2417
30.00
32.00
P3
5
26.8000
1.30384
.58310
25.1811
28.4189
25.00
28.00
25
31.0400
3.55293
.71059
29.5734
32.5066
25.00
37.00
Total
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Test of Homogeneity of Variances
berat badan H20
Levene Statistic
df1
8.107
df2
4
Sig.
20
.000
ANOVA
berat badan H20
Sum of Squares
df
Mean Square
Between Groups
168.960
4
42.240
Within Groups
134.000
20
6.700
Total
302.960
24
F
Sig.
6.304
.002
berat badan H20
a
Duncan
perlakuan
N
Subset for alpha = 0.05
1
2
3
P3
5
26.8000
P1
5
30.2000
P2
5
31.0000
K1
5
32.6000
KO
5
30.2000
32.6000
34.6000
Sig.
.051
.180
.236
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
d. Berat Badan Hari ke- 30
Descriptives
berat badan H30
N
Mean
Std.
Std.
95% Confidence
Deviation
Error
Interval for Mean
Lower
Upper
Bound
Bound
Minimum
Maximum
KO
5 36.6000
3.84708
1.72047 31.8232
41.3768
30.00
40.00
K1
5 32.4000
4.82701
2.15870 26.4065
38.3935
25.00
36.00
P1
5 28.2000
1.92354
.86023 25.8116
30.5884
26.00
31.00
P2
5 30.4000
.54772
.24495 29.7199
31.0801
30.00
31.00
P3
5 26.2000
2.16795
.96954 23.5081
28.8919
23.00
28.00
25 30.7600
4.60326
.92065 28.8599
32.6601
23.00
40.00
Total
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Test of Homogeneity of Variances
berat badan H30
Levene Statistic
df1
3.487
df2
4
Sig.
20
.026
ANOVA
berat badan H30
Sum of Squares
df
Mean Square
Between Groups
321.360
4
80.340
Within Groups
187.200
20
9.360
Total
508.560
24
F
8.583
Sig.
.000
berat badan H30
a
Duncan
perlakuan
N
Subset for alpha = 0.05
1
2
3
P3
5
26.2000
P1
5
28.2000
28.2000
P2
5
30.4000
30.4000
K1
5
KO
5
Sig.
32.4000
36.6000
.052
.052
1.000
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Lampiran 7. Analisis Statistik Kadar Kolesterol Total Mencit Setelah Pemberian
Diet Kuning Telur Dan Ekstrak Daun Pirdot Selama 30 Hari.
a. Kadar Kolesterol Total Hari ke- 0
Descriptives
kolesterol total H0
N
Mean
Std.
Std.
95% Confidence
Deviation
Error
Interval for Mean
KO
5 127.8000
27.83343 12.44749
K1
5 124.6000
19.26915
P1
5 111.8000
P2
P3
Total
Minimum
Lower
Upper
Bound
Bound
Maximum
93.2402
162.3598
105.00
176.00
8.61742
100.6742
148.5258
109.00
157.00
11.98749
5.36097
96.9156
126.6844
102.00
132.00
5 111.8000
8.70057
3.89102
100.9968
122.6032
100.00
123.00
5 116.4000
7.53658
3.37046
107.0421
125.7579
105.00
125.00
25 118.4800
16.80555
3.36111
111.5430
125.4170
100.00
176.00
Test of Homogeneity of Variances
kolesterol total H0
Levene Statistic
1.511
df1
df2
4
Sig.
20
.237
ANOVA
kolesterol total H0
Sum of Squares
Df
Mean Square
Between Groups
1089.440
4
272.360
Within Groups
5688.800
20
284.440
Total
6778.240
24
F
Sig.
.958
.452
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b. Kadar Kolesterol Total Hari ke- 10
Descriptives
kolesterol total H10
N
Mean
Std.
Std.
95% Confidence
Deviation
Error
Interval for Mean
Lower
Upper
Bound
Bound
Minimum
Maximum
KO
5
151.4000
23.76552 10.62826
121.8912
180.9088
134.00
191.00
K1
5
144.2000
22.94995 10.26353
115.7039
172.6961
117.00
167.00
P1
5
185.8000
11.43241
5.11273
171.6048
199.9952
172.00
199.00
P2
5
192.0000
30.50410 13.64185
154.1242
229.8758
159.00
225.00
P3
5
212.8000
42.27529 18.90608
160.3083
265.2917
167.00
281.00
25
177.2400
36.70522
162.0888
192.3912
117.00
281.00
Total
7.34104
Test of Homogeneity of Variances
kolesterol total H10
Levene Statistic
df1
1.107
df2
4
Sig.
20
.381
ANOVA
kolesterol total H10
Sum of Squares
df
Mean Square
Between Groups
16574.960
4
4143.740
Within Groups
15759.600
20
787.980
Total
32334.560
24
F
5.259
Sig.
.005
kolesterol total H10
a
Duncan
perlakuan
N
Subset for alpha = 0.05
1
2
3
K1
5
144.2000
KO
5
151.4000
P1
5
P2
5
192.0000
P3
5
212.8000
Sig.
151.4000
185.8000
.689
185.8000
.067
.165
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
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c. Kadar Kolesterol Total Hari ke- 20
Descriptives
kolesterol total H20
N
Mean
Std.
Std. Error
95% Confidence
Deviation
Minimum Maximum
Interval for Mean
Lower
Upper
Bound
Bound
KO
5 142.4000
38.21387
17.08976
94.9512 189.8488
107.00
207.00
K1
5 186.0000
62.88879
28.12472 107.9133 264.0867
127.00
279.00
P1
5 156.0000
14.01785
6.26897 138.5945 173.4055
143.00
176.00
P2
5 166.0000
34.13942
15.26761 123.6103 208.3897
125.00
206.00
P3
5 172.4000
44.07720
19.71193 117.6709 227.1291
132.00
245.00
25 164.5600
40.98687
8.19737 147.6415 181.4785
107.00
279.00
Total
Test of Homogeneity of Variances
kolesterol total H20
Levene Statistic
2.095
df1
df2
4
Sig.
20
.119
ANOVA
kolesterol total H20
Sum of Squares
Between Groups
df
Mean Square
5437.760
4
1359.440
Within Groups
34880.400
20
1744.020
Total
40318.160
24
F
Sig.
.779
.552
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d. Kadar Kolesterol Total Hari ke- 30
Descriptives
kolesterol total H30
N
Mean
Std.
Std.
95% Confidence
Deviation
Error
Interval for Mean
Lower
Upper
Bound
Bound
Minimum
Maximum
KO
5 130.0000 20.71232
9.26283 104.2823 155.7177
105.00
154.00
K1
5 205.2000 35.74493 15.98562 160.8168 249.5832
149.00
239.00
P1
5 145.6000 10.78425
132.00
158.00
P2
5 135.6000 29.24551 13.07899
99.2869 171.9131
110.00
182.00
P3
5 157.0000 38.63936 17.28005 109.0229 204.9771
125.00
224.00
105.00
239.00
Total
4.82286 132.2096 158.9904
25 154.6800 38.05229
7.61046 138.9728 170.3872
Test of Homogeneity of Variances
kolesterol total H30
Levene Statistic
df1
1.281
df2
4
Sig.
20
.311
ANOVA
kolesterol total H30
Sum of Squares
df
Mean Square
Between Groups
18066.240
4
4516.560
Within Groups
16685.200
20
834.260
Total
34751.440
24
F
5.414
Sig.
.004
kolesterol total H30
a
Duncan
perlakuan
N
Subset for alpha = 0.05
1
2
KO
5
130.0000
P2
5
135.6000
P1
5
145.6000
P3
5
157.0000
K1
5
Sig.
205.2000
.190
1.000
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
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Lampiran 8. Analisis Statistik Kadar Trigliserida Mencit Setelah Pemberian Diet
Kuning Telur Dan Ekstrak Daun Pirdot Selama 30 Hari
Descriptives
Trigliserida
N
Mean
Std.
Std.
95% Confidence
Deviation
Error
Interval for Mean
Lower
Upper
Bound
Bound
Minimum
Maximum
KO
5
104.6000 26.74509 11.96077
71.3916
137.8084
77.00
144.00
K1
5
132.0000 55.33986 24.74874
63.2865
200.7135
56.00
193.00
P1
5
145.4000 36.46642 16.30828 100.1210
190.6790
107.00
194.00
P2
5
123.0000 47.86961 21.40794
63.5620
182.4380
76.00
179.00
P3
5
141.6000 50.51039 22.58894
78.8831
204.3169
105.00
227.00
8.67366 111.4184
147.2216
56.00
227.00
Total
25
129.3200 43.36831
Test of Homogeneity of Variances
Trigliserida
Levene Statistic
df1
.791
df2
4
Sig.
20
.545
ANOVA
Trigliserida
Sum of Squares
Between Groups
df
Mean Square
5337.840
4
1334.460
Within Groups
39801.600
20
1990.080
Total
45139.440
24
F
Sig.
.671
.620
Trigliserida
a
Duncan
Perlakuan
N
Subset for alpha = 0.05
1
KO
5
104.6000
P2
5
123.0000
K1
5
132.0000
P3
5
141.6000
P1
5
145.4000
Sig.
.208
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
39
Lampiran 9. Analisis Statistik Kadar HDL Mencit Setelah Pemberian Diet Kuning
Telur Dan Ekstrak Daun Pirdot Selama 30 Hari
Descriptives
HDL
N
Mean
Std.
Std.
95% Confidence
Deviation
Error
Interval for Mean
Minimum
Maximum
Lower
Upper
Bound
Bound
56.1405
77.4595
59.00
80.00
KO
5
66.8000
8.58487 3.83927
K1
5 105.4000
20.48902 9.16297
79.9595 130.8405
82.00
128.00
P1
5
77.2000
11.84061 5.29528
62.4979
91.9021
65.00
91.00
P2
5
49.8000
8.78635 3.92938
38.8903
60.7097
40.00
64.00
P3
5
77.0000
12.00000 5.36656
62.1000
91.9000
59.00
89.00
25
75.2400
21.97969 4.39594
66.1672
84.3128
40.00
128.00
Total
Test of Homogeneity of Variances
HDL
Levene Statistic
df1
2.328
df2
4
Sig.
20
.091
ANOVA
HDL
Sum of Squares
df
Mean Square
Between Groups
8174.960
4
2043.740
Within Groups
3419.600
20
170.980
11594.560
24
Total
F
11.953
Sig.
.000
HDL
a
Duncan
Perlakuan
N
Subset for alpha = 0.05
1
2
3
P2
5
49.8000
KO
5
66.8000
P3
5
77.0000
P1
5
77.2000
K1
5
Sig.
66.8000
105.4000
.053
.248
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
40
Lampiran 8. Analisis Statistik Kadar LDL Mencit Setelah Pemberian Diet Kuning
Telur Dan Ekstrak Daun Pirdot Selama 30 Hari
Descriptives
LDL
N
Mean
Std.
Std. Error
95% Confidence
Deviation
Minimum
Maximum
Interval for Mean
Lower
Upper
Bound
Bound
KO
5 42.4000 18.76966
8.39405
19.0944
65.7056
18.00
68.00
K1
5 93.4000 18.07761
8.08455
70.9537 115.8463
76.00
120.00
P1
5 39.0000 13.61984
6.09098
22.0887
55.9113
25.00
57.00
P2
5 61.4000 23.47978
10.50048
32.2460
90.5540
40.00
91.00
P3
5 52.0000 54.09713
24.19297
-15.1705 119.1705
9.00
144.00
25 57.6400 33.47372
6.69474
9.00
144.00
Total
43.8227
71.4573
Test of Homogeneity of Variances
LDL
Levene Statistic
df1
1.802
df2
4
Sig.
20
.168
ANOVA
LDL
Sum of Squares
Between Groups
df
Mean Square
9522.160
4
2380.540
Within Groups
17369.600
20
868.480
Total
26891.760
24
F
2.741
Sig.
.057
LDL
a
Duncan
Perlakuan
N
Subset for alpha = 0.05
1
2
P1
5
39.0000
KO
5
42.4000
P3
5
52.0000
P2
5
61.4000
K1
5
Sig.
61.4000
93.4000
.284
.101
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 1. Komposisi Kuning Telur Puyuh
Informasi Rinci Komposisi Kandungan Nutrisi/Gizi Pada Telur Puyuh :
Berat Telur Puyuh yang diteliti (Food Weight)
100 g
Bagian Telur yang dapat dikonsumsi (Bdd / Food Edible) 100 %
Kandungan Energi
168 kkal
Kandungan Protein
12,3 g
Kandungan Lemak
12,7 g
Kandungan Karbohidrat
1,2 g
Kandungan Kalsium
64 mg
Kandungan Fosfor
226 mg
Kandungan Zat Besi
3,65 mg
Kandungan Vitamin A
543 IU
Kandungan Vitamin B12
1,58 mg
Kandungan Vitamin E
1,08 mg
Kandungan Kolin
263,4 mg
Sumber Informasi Gizi : Berbagai publikasi Kementerian Kesehatan
Republik Indonesia
Universitas Sumatera Utara
26
Lampiran 2. Skrining fitokimia ektrak etanol daun pirdot (Saurauia vulcani .K)
(Sitorus, 2015)
No
Skrining
Simplisia
Ekstrak Etanol
1.
Alkanoid
-
-
2.
Flavonoid
+
+
3.
Glikosida
+
+
4.
Saponin
+
+
5.
Antraquinonglikosida
-
-
6.
Tanin
+
+
7.
Terpenoid/ steroid
+
+
Ket : Ada (+)
Tidak ada (-)
Universitas Sumatera Utara
27
Lampiran 3. Data Berat Badan mencit yang diberi diet kuning telur dan ekstrak
daun pirdot selama 30 hari.
a. Berat Badan Hari ke- 0
Perlakuan
KO
K1
P1
P2
P3
U1
26
26
25
26
25
U2
28
35
28
25
27
Ulangan
U3
U4
26
32
29
26
26
28
29
29
26
25
U5
25
25
29
25
28
Rata
Rata
STDEV
26.6
29.4
28
25.6
26.2
1.341641
4.159327
1.732051
0.547723
1.30384
Rata
Rata
STDEV
31.2
29.6
33.8
32.8
29.4
1.30384
5.59464
1.30384
1.48324
2.073644
Rata
Rata
STDEV
34.6
32.6
30.2
31
26.8
2.302173
4.827007
1.48324
1
1.30384
Rata
Rata
STDEV
36.6
32.4
28.2
30.4
26.2
3.847077
4.827007
1.923538
0.547723
2.167948
b. Berat Badan Hari ke- 10
Perlakuan
KO
K1
P1
P2
P3
U1
32
27
32
32
27
U2
33
26
33
31
29
Ulangan
U3
U4
30
34
35
33
31
30
37
35
35
28
U5
31
24
34
33
32
c. Berat Badan Hari ke- 20
Perlakuan
KO
K1
P1
P2
P3
U1
37
29
28
30
25
U2
36
36
30
30
26
Ulangan
U3
U4
35
35
31
32
28
31
37
32
32
27
U5
34
26
30
31
28
d. Berat Badan Hari ke- 30
Perlakuan
KO
K1
P1
P2
P3
U1
40
30
26
31
23
U2
37
35
27
30
25
Ulangan
U3
U4
38
36
29
30
28
30
36
31
31
27
U5
38
25
28
30
28
Universitas Sumatera Utara
28
Lampiran 4. Data Kadar Kolesterol Total mencit setela pemberian diet kung telur
dan ekstrak daun pirdot selama 30 hari.
a. Kadar Kolesterol Total Hari ke-0
Ulangan
Perlakuan
U1
U2
U3
U4
KO
K1
P1
P2
P3
124
112
105
109
116
115
119
102
117
105
105
109
113
123
121
176
157
107
110
125
b. Kadar Kolesterol Total Hari ke-10
Ulangan
Perlakuan
U1
U2
U3
U4
KO
K1
P1
P2
P3
140
117
172
210
210
134
122
193
160
193
191
167
189
225
213
156
158
176
206
281
c. Kadar Kolesterol Total Hari ke-20
Ulangan
Perlakuan
U1
U2
U3
U4
KO
K1
P1
P2
P3
134
127
150
196
132
140
279
146
146
143
107
145
165
206
171
207
158
143
157
245
d. Kadar Kolesterol Total Hari ke-10
Ulangan
Perlakuan
U1
U2
U3
U4
KO
K1
P1
P2
P3
Ket :
129
149
140
123
125
KO
K1
P1
P2
P3
115
224
143
117
140
105
222
158
182
146
154
192
132
146
224
U5
119
126
132
100
115
U5
136
157
199
159
167
U5
124
221
176
125
171
U5
147
239
155
110
150
Rata
Rata
STDEV
127.8
124.6
111.8
111.8
116.4
27.83343
19.26915
11.98749
8.700575
7.536577
Rata
Rata
STDEV
151.4
144.2
185.8
192
212.8
23.76552
22.94995
11.43241
30.5041
42.27529
Rata
Rata
STDEV
142.4
186
156
166
172.4
38.21387
62.88879
14.01785
34.13942
44.0772
Rata
Rata
STDEV
130
205.2
145.6
135.6
157
20.71232
35.74493
10.78425
29.24551
38.63936
: Kontrol Negatif
: Kontrol Positif
: Ekstrak Daun Pirdot 100 mg/kgBB
: Ekstrak Daun Pirdot 200 mg/kgBB
: Ekstrak Daun Pirdot 300 mg/kgBB
Universitas Sumatera Utara
29
Lampiran 5. Data Kadar Trigliserida, HDL, dan LDL Serum Darah Mencit
Setelah Pemberian Diet Kuning Telur Dan Ekstrak Daun Pirdot
Selama 30 Hari.
a. Trigliserida
Perlakuan
KO
K1
P1
P2
P3
U1
77
175
164
167
148
U2
118
102
150
81
112
Ulangan
U3
87
193
112
179
116
U4
144
134
107
76
105
U5
97
56
194
112
227
Rata Rata
104.6
132
145.4
123
141.6
STDEV
26.7451
55.3399
36.4664
47.8696
50.5104
U2
59
128
87
50
75
Ulangan
U3
70
82
77
64
75
U4
80
87
65
40
59
U5
60
108
91
48
89
Rata Rata
66.8
105.4
77.2
49.8
77
STDEV
8.58487
20.489
11.8406
8.78635
12
U2
32
76
26
51
43
Ulangan
U3
18
101
57
82
48
U4
45
78
46
91
144
U5
68
120
25
40
16
Rata Rata
42.4
93.4
39
61.4
52
STDEV
18.7697
18.0776
13.6198
23.4798
54.0971
b. HDL
Perlakuan
KO
K1
P1
P2
P3
U1
65
122
66
47
87
c. LDL
Perlakuan
KO
K1
P1
P2
P3
Ket :
KO
K1
P1
P2
P3
U1
49
92
41
43
9
: Kontrol Negatif
: Kontrol Positif
: Ekstrak Daun Pirdot 100 mg/kgBB
: Ekstrak Daun Pirdot 200 mg/kgBB
: Ekstrak Daun Pirdot 300 mg/kgBB
Universitas Sumatera Utara
30
Lampiran 6. Analisis Statistik Berat Badan Mencit Setelah Pemberian Diet
Kuning Telur Dan Ekstrak Daun Pirdot Selama 30 Hari.
a. Berat Badan Hari ke-0
Descriptives
berat badan H0
N
Mean
Std.
Std. Error
95% Confidence
Deviation
Minimum Maximum
Interval for Mean
Lower
Upper
Bound
Bound
KO
5 26.6000
1.34164
.60000
24.9341
28.2659
25.00
28.00
K1
5 29.4000
4.15933
1.86011
24.2355
34.5645
25.00
35.00
P1
5 28.0000
1.73205
.77460
25.8494
30.1506
25.00
29.00
P2
5 25.6000
.54772
.24495
24.9199
26.2801
25.00
26.00
P3
5 26.2000
1.30384
.58310
24.5811
27.8189
25.00
28.00
25 27.1600
2.44404
.48881
26.1511
28.1689
25.00
35.00
Total
Test of Homogeneity of Variances
berat badan H0
Levene Statistic
5.123
df1
df2
4
Sig.
20
.005
ANOVA
berat badan H0
Sum of Squares
df
Mean Square
Between Groups
46.960
4
11.740
Within Groups
96.400
20
4.820
143.360
24
Total
F
2.436
Sig.
.081
Universitas Sumatera Utara
31
b. Berat badan Hari ke-10
Descriptives
berat badan H10
N
Mean
Std.
Std. Error
95% Confidence
Deviation
Minimum
Maximum
Interval for Mean
Lower
Upper
Bound
Bound
KO
5 31.2000
1.30384
.58310
29.5811
32.8189
30.00
33.00
K1
5 29.6000
5.59464
2.50200
22.6533
36.5467
24.00
37.00
P1
5 33.8000
1.30384
.58310
32.1811
35.4189
32.00
35.00
P2
5 32.8000
1.48324
.66332
30.9583
34.6417
31.00
35.00
P3
5 29.4000
2.07364
.92736
26.8252
31.9748
27.00
32.00
25 31.3600
3.16070
.63214
30.0553
32.6647
24.00
37.00
Total
Test of Homogeneity of Variances
berat badan H10
Levene Statistic
df1
11.074
df2
4
Sig.
20
.000
ANOVA
berat badan H10
Sum of Squares
Between Groups
df
Mean Square
74.960
4
18.740
Within Groups
164.800
20
8.240
Total
239.760
24
F
Sig.
2.274
.097
c. Berat Badan Hari ke-20
Descriptives
berat badan H20
N
Mean
Std.
Std. Error
Deviation
95% Confidence
Minimum
Maximum
Interval for Mean
Lower
Upper
Bound
Bound
KO
5
34.6000
2.30217
1.02956
31.7415
37.4585
31.00
37.00
K1
5
32.6000
4.82701
2.15870
26.6065
38.5935
26.00
37.00
P1
5
30.2000
1.48324
.66332
28.3583
32.0417
28.00
32.00
P2
5
31.0000
1.00000
.44721
29.7583
32.2417
30.00
32.00
P3
5
26.8000
1.30384
.58310
25.1811
28.4189
25.00
28.00
25
31.0400
3.55293
.71059
29.5734
32.5066
25.00
37.00
Total
Universitas Sumatera Utara
32
Test of Homogeneity of Variances
berat badan H20
Levene Statistic
df1
8.107
df2
4
Sig.
20
.000
ANOVA
berat badan H20
Sum of Squares
df
Mean Square
Between Groups
168.960
4
42.240
Within Groups
134.000
20
6.700
Total
302.960
24
F
Sig.
6.304
.002
berat badan H20
a
Duncan
perlakuan
N
Subset for alpha = 0.05
1
2
3
P3
5
26.8000
P1
5
30.2000
P2
5
31.0000
K1
5
32.6000
KO
5
30.2000
32.6000
34.6000
Sig.
.051
.180
.236
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
d. Berat Badan Hari ke- 30
Descriptives
berat badan H30
N
Mean
Std.
Std.
95% Confidence
Deviation
Error
Interval for Mean
Lower
Upper
Bound
Bound
Minimum
Maximum
KO
5 36.6000
3.84708
1.72047 31.8232
41.3768
30.00
40.00
K1
5 32.4000
4.82701
2.15870 26.4065
38.3935
25.00
36.00
P1
5 28.2000
1.92354
.86023 25.8116
30.5884
26.00
31.00
P2
5 30.4000
.54772
.24495 29.7199
31.0801
30.00
31.00
P3
5 26.2000
2.16795
.96954 23.5081
28.8919
23.00
28.00
25 30.7600
4.60326
.92065 28.8599
32.6601
23.00
40.00
Total
Universitas Sumatera Utara
33
Test of Homogeneity of Variances
berat badan H30
Levene Statistic
df1
3.487
df2
4
Sig.
20
.026
ANOVA
berat badan H30
Sum of Squares
df
Mean Square
Between Groups
321.360
4
80.340
Within Groups
187.200
20
9.360
Total
508.560
24
F
8.583
Sig.
.000
berat badan H30
a
Duncan
perlakuan
N
Subset for alpha = 0.05
1
2
3
P3
5
26.2000
P1
5
28.2000
28.2000
P2
5
30.4000
30.4000
K1
5
KO
5
Sig.
32.4000
36.6000
.052
.052
1.000
Universitas Sumatera Utara
34
Lampiran 7. Analisis Statistik Kadar Kolesterol Total Mencit Setelah Pemberian
Diet Kuning Telur Dan Ekstrak Daun Pirdot Selama 30 Hari.
a. Kadar Kolesterol Total Hari ke- 0
Descriptives
kolesterol total H0
N
Mean
Std.
Std.
95% Confidence
Deviation
Error
Interval for Mean
KO
5 127.8000
27.83343 12.44749
K1
5 124.6000
19.26915
P1
5 111.8000
P2
P3
Total
Minimum
Lower
Upper
Bound
Bound
Maximum
93.2402
162.3598
105.00
176.00
8.61742
100.6742
148.5258
109.00
157.00
11.98749
5.36097
96.9156
126.6844
102.00
132.00
5 111.8000
8.70057
3.89102
100.9968
122.6032
100.00
123.00
5 116.4000
7.53658
3.37046
107.0421
125.7579
105.00
125.00
25 118.4800
16.80555
3.36111
111.5430
125.4170
100.00
176.00
Test of Homogeneity of Variances
kolesterol total H0
Levene Statistic
1.511
df1
df2
4
Sig.
20
.237
ANOVA
kolesterol total H0
Sum of Squares
Df
Mean Square
Between Groups
1089.440
4
272.360
Within Groups
5688.800
20
284.440
Total
6778.240
24
F
Sig.
.958
.452
Universitas Sumatera Utara
35
b. Kadar Kolesterol Total Hari ke- 10
Descriptives
kolesterol total H10
N
Mean
Std.
Std.
95% Confidence
Deviation
Error
Interval for Mean
Lower
Upper
Bound
Bound
Minimum
Maximum
KO
5
151.4000
23.76552 10.62826
121.8912
180.9088
134.00
191.00
K1
5
144.2000
22.94995 10.26353
115.7039
172.6961
117.00
167.00
P1
5
185.8000
11.43241
5.11273
171.6048
199.9952
172.00
199.00
P2
5
192.0000
30.50410 13.64185
154.1242
229.8758
159.00
225.00
P3
5
212.8000
42.27529 18.90608
160.3083
265.2917
167.00
281.00
25
177.2400
36.70522
162.0888
192.3912
117.00
281.00
Total
7.34104
Test of Homogeneity of Variances
kolesterol total H10
Levene Statistic
df1
1.107
df2
4
Sig.
20
.381
ANOVA
kolesterol total H10
Sum of Squares
df
Mean Square
Between Groups
16574.960
4
4143.740
Within Groups
15759.600
20
787.980
Total
32334.560
24
F
5.259
Sig.
.005
kolesterol total H10
a
Duncan
perlakuan
N
Subset for alpha = 0.05
1
2
3
K1
5
144.2000
KO
5
151.4000
P1
5
P2
5
192.0000
P3
5
212.8000
Sig.
151.4000
185.8000
.689
185.8000
.067
.165
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
36
c. Kadar Kolesterol Total Hari ke- 20
Descriptives
kolesterol total H20
N
Mean
Std.
Std. Error
95% Confidence
Deviation
Minimum Maximum
Interval for Mean
Lower
Upper
Bound
Bound
KO
5 142.4000
38.21387
17.08976
94.9512 189.8488
107.00
207.00
K1
5 186.0000
62.88879
28.12472 107.9133 264.0867
127.00
279.00
P1
5 156.0000
14.01785
6.26897 138.5945 173.4055
143.00
176.00
P2
5 166.0000
34.13942
15.26761 123.6103 208.3897
125.00
206.00
P3
5 172.4000
44.07720
19.71193 117.6709 227.1291
132.00
245.00
25 164.5600
40.98687
8.19737 147.6415 181.4785
107.00
279.00
Total
Test of Homogeneity of Variances
kolesterol total H20
Levene Statistic
2.095
df1
df2
4
Sig.
20
.119
ANOVA
kolesterol total H20
Sum of Squares
Between Groups
df
Mean Square
5437.760
4
1359.440
Within Groups
34880.400
20
1744.020
Total
40318.160
24
F
Sig.
.779
.552
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37
d. Kadar Kolesterol Total Hari ke- 30
Descriptives
kolesterol total H30
N
Mean
Std.
Std.
95% Confidence
Deviation
Error
Interval for Mean
Lower
Upper
Bound
Bound
Minimum
Maximum
KO
5 130.0000 20.71232
9.26283 104.2823 155.7177
105.00
154.00
K1
5 205.2000 35.74493 15.98562 160.8168 249.5832
149.00
239.00
P1
5 145.6000 10.78425
132.00
158.00
P2
5 135.6000 29.24551 13.07899
99.2869 171.9131
110.00
182.00
P3
5 157.0000 38.63936 17.28005 109.0229 204.9771
125.00
224.00
105.00
239.00
Total
4.82286 132.2096 158.9904
25 154.6800 38.05229
7.61046 138.9728 170.3872
Test of Homogeneity of Variances
kolesterol total H30
Levene Statistic
df1
1.281
df2
4
Sig.
20
.311
ANOVA
kolesterol total H30
Sum of Squares
df
Mean Square
Between Groups
18066.240
4
4516.560
Within Groups
16685.200
20
834.260
Total
34751.440
24
F
5.414
Sig.
.004
kolesterol total H30
a
Duncan
perlakuan
N
Subset for alpha = 0.05
1
2
KO
5
130.0000
P2
5
135.6000
P1
5
145.6000
P3
5
157.0000
K1
5
Sig.
205.2000
.190
1.000
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
38
Lampiran 8. Analisis Statistik Kadar Trigliserida Mencit Setelah Pemberian Diet
Kuning Telur Dan Ekstrak Daun Pirdot Selama 30 Hari
Descriptives
Trigliserida
N
Mean
Std.
Std.
95% Confidence
Deviation
Error
Interval for Mean
Lower
Upper
Bound
Bound
Minimum
Maximum
KO
5
104.6000 26.74509 11.96077
71.3916
137.8084
77.00
144.00
K1
5
132.0000 55.33986 24.74874
63.2865
200.7135
56.00
193.00
P1
5
145.4000 36.46642 16.30828 100.1210
190.6790
107.00
194.00
P2
5
123.0000 47.86961 21.40794
63.5620
182.4380
76.00
179.00
P3
5
141.6000 50.51039 22.58894
78.8831
204.3169
105.00
227.00
8.67366 111.4184
147.2216
56.00
227.00
Total
25
129.3200 43.36831
Test of Homogeneity of Variances
Trigliserida
Levene Statistic
df1
.791
df2
4
Sig.
20
.545
ANOVA
Trigliserida
Sum of Squares
Between Groups
df
Mean Square
5337.840
4
1334.460
Within Groups
39801.600
20
1990.080
Total
45139.440
24
F
Sig.
.671
.620
Trigliserida
a
Duncan
Perlakuan
N
Subset for alpha = 0.05
1
KO
5
104.6000
P2
5
123.0000
K1
5
132.0000
P3
5
141.6000
P1
5
145.4000
Sig.
.208
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
39
Lampiran 9. Analisis Statistik Kadar HDL Mencit Setelah Pemberian Diet Kuning
Telur Dan Ekstrak Daun Pirdot Selama 30 Hari
Descriptives
HDL
N
Mean
Std.
Std.
95% Confidence
Deviation
Error
Interval for Mean
Minimum
Maximum
Lower
Upper
Bound
Bound
56.1405
77.4595
59.00
80.00
KO
5
66.8000
8.58487 3.83927
K1
5 105.4000
20.48902 9.16297
79.9595 130.8405
82.00
128.00
P1
5
77.2000
11.84061 5.29528
62.4979
91.9021
65.00
91.00
P2
5
49.8000
8.78635 3.92938
38.8903
60.7097
40.00
64.00
P3
5
77.0000
12.00000 5.36656
62.1000
91.9000
59.00
89.00
25
75.2400
21.97969 4.39594
66.1672
84.3128
40.00
128.00
Total
Test of Homogeneity of Variances
HDL
Levene Statistic
df1
2.328
df2
4
Sig.
20
.091
ANOVA
HDL
Sum of Squares
df
Mean Square
Between Groups
8174.960
4
2043.740
Within Groups
3419.600
20
170.980
11594.560
24
Total
F
11.953
Sig.
.000
HDL
a
Duncan
Perlakuan
N
Subset for alpha = 0.05
1
2
3
P2
5
49.8000
KO
5
66.8000
P3
5
77.0000
P1
5
77.2000
K1
5
Sig.
66.8000
105.4000
.053
.248
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
40
Lampiran 8. Analisis Statistik Kadar LDL Mencit Setelah Pemberian Diet Kuning
Telur Dan Ekstrak Daun Pirdot Selama 30 Hari
Descriptives
LDL
N
Mean
Std.
Std. Error
95% Confidence
Deviation
Minimum
Maximum
Interval for Mean
Lower
Upper
Bound
Bound
KO
5 42.4000 18.76966
8.39405
19.0944
65.7056
18.00
68.00
K1
5 93.4000 18.07761
8.08455
70.9537 115.8463
76.00
120.00
P1
5 39.0000 13.61984
6.09098
22.0887
55.9113
25.00
57.00
P2
5 61.4000 23.47978
10.50048
32.2460
90.5540
40.00
91.00
P3
5 52.0000 54.09713
24.19297
-15.1705 119.1705
9.00
144.00
25 57.6400 33.47372
6.69474
9.00
144.00
Total
43.8227
71.4573
Test of Homogeneity of Variances
LDL
Levene Statistic
df1
1.802
df2
4
Sig.
20
.168
ANOVA
LDL
Sum of Squares
Between Groups
df
Mean Square
9522.160
4
2380.540
Within Groups
17369.600
20
868.480
Total
26891.760
24
F
2.741
Sig.
.057
LDL
a
Duncan
Perlakuan
N
Subset for alpha = 0.05
1
2
P1
5
39.0000
KO
5
42.4000
P3
5
52.0000
P2
5
61.4000
K1
5
Sig.
61.4000
93.4000
.284
.101
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara