B1J010115 14.
Lampiran 1. Spesifikasi Bahan Dan Peralatan
No.
1.
Nama Alat
Akuarium
Merek/Tipe
Kegunaan
Tempat pemeliharaan
ikan uji
2.
Aerator
Amara
Sumber oksigen
3.
Timbangan
analitik
Ohaus
4.
Gelas ukur
Iwaki TE32 PYREX
Menimbang bubuk
metiltestosteron dan
aliuzarin red
Mengukur volume
larutan
5.
Beaker glass
Tempat menampung
larutan
6.
Milimeter blok
Iwaki,
PYREX
50ml
-
7.
Pipet tetes
-
Untuk memindahkan
larutan
8.
Timbangan
analitik
CHQ
Menimbang berat
ikan uji
9.
Piring kecil
-
10.
Mikroskop
binokuler
Olympus
CH20
11.
Termostat
12.
Spuit injeksi
Sobo
Akuaking
Amara
OneMed
1ml
Tempat
mencampurkan milt
dan sel telur
Mengamati jaringan
histologis sirip ikan
uji
Setting temperatur
13.
Saringan
Mengukur panjang
tubuh ikan uji
Untuk injeksi
ovaprim ke tubuh
induk ikan uji
Memisahkan sel telur
dengan seminal
plasma sperma pada
saat pemijahan
bio.unsoed.ac.id
-
44
Tempat
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
No.
Nama Alat
Merek/Tipe
Kegunaan
Tempat
14.
Kamera
Nikon
Mendokumentasikan
jaringan histologis
sirip
Lab. Struktur
Perkembangan
Hewan
15.
Eye piece
micrometer
Olympus
Mengukur panjang dan
luas sirip ikan uji
Lab. Struktur
Perkembangan
Hewan
16.
Botol sampel
-
Meletakan sampel
sirip ikan uji
17.
Kertas label
-
Mencatat informasi
sampel
18.
Cavity slide
-
Meletakkan ikan yang
akan diamati dibawah
mikrooskop
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
19.
Magnetic stirer
Menghomogenkan
larutan alizarin red,
penjernih A, B, dan C
bio.unsoed.ac.id
44
Lab. Fisiologi
Tumbuhan
No.
1.
Nama Bahan
Ovaprim
Spesifikasi
Syndel 10ml
2.
Alkohol 70%
C2H5OH Mol: 46,07 g/mol
3.
17αMetiltestosteron
Mol:302,451 mg/L
4.
Pelet
Protein 41%, Lemak 6%, Serat 2,5%,
Abu 16%, Kelembaban 11%
5.
KOH 1%
Menjernihkan
ikan uji
6.
Asam asetat glasial
7.
Alizarin Red
8.
Gliserin PA
9.
Alkohol 1%
10.
KOH 2%
11.
KOH 4%
12.
Gliserin
13.
14.
15.
16.
Alkohol 96%
Akuades
Larva ikan nilem
Induk ikan nilem
Pembuatan larutan
alizarin red
Mewarnai sirip ikan
uji
Pembuatan pewarna
alizarin red
Pembuatan pewarna
alizarin red
Pelarut alizarin red
preparat histologis
Pembuatan larutan
penjernih A dan B
Pembuatan larutan
penjernih A, B dan C
Fiksasi ikan uji
Pelarut
Ikan uji
Penyedia ikan uji
C14H8O4. pH 5,8-7,2
Usia 1 hari penetasan
Matang gonad
bio.unsoed.ac.id
44
Kegunaan
Induksi induk ikan
uji
Pelarut
metiltestosteron
Larutan yang
digunakan sebagai
medium
pemeliharaan ikan uji
Pakan ikan uji
otot
Lampiran 2. Diagram alir penelitian
Pemijahan dan Penyediaan Ikan Uji
Persiapan Bak Pemeliharaan
Setting temperatur
Pembuatan Medium Pemeliharaan Ikan uji
Pemeliharaan Ikan Uji
Pengambilan data
perkembangan sirip secara
mikroskopis
Evaluasi Perkembangan sirip
secara makroskopis
Pewarnaan sirip
menggunakan metode
alizarin red
Analisis Data
Evaluasi hasil pewarnaan
bio.unsoed.ac.id
44
Lampiran 3. Hasil analisis ANOVA panjang dan luas sirip caudal ikan Nilem
(O. hasselti) yang dipelihara dalam medium dengan temperatur
dan kadar metiltestosteron berbeda.
1. Panjang Sirip Caudal
Tests of Between-Subjects Effects
Dependent Variable:PANJANG SIRIP CAUDAL
Type III Sum
of Squares
Source
Intercept
Hypothesis
Error
Suhu
Hypothesis
Error
Suhu * Ulangan
Hypothesis
Error
Hormon
Hypothesis
Error
Suhu * Hormon
Hypothesis
Error
a. MS(suhu * ulangan)
b. MS(Error)
df
Mean Square
6.706E7
1345985.642
260677.867
1345985.642
1345985.642
3202939.998
846965.477
3202939.998
3178602.112
3202939.998
F
1
6.706E7
6
2
6
6
18
3
18
6
18
a
Sig.
298.947
.000
.581
.588
1.261
.323
1.587
.227
2.977
.034
224330.940
130338.934
224330.940a
224330.940
177941.111b
282321.826
177941.111b
529767.019
177941.111b
Descriptives
Panjang Sirip Caudal
Interaksi
perlakuan
N
Mean
Std.
Deviation
95% Confidence Interval
for Mean
Std. Error
Minimum Maximum
Lower
Bound
t1m1
3 1.1723E3 431.43603 2.49090E2
t1m2
3 1.6596E3
t1m3
3 1.0961E3 207.11204 1.19576E2
t1m4
Upper Bound
100.5735
2244.0665
718.36
1577.00
51.85859 1436.4838
1882.7428
1574.30
1753.35
581.6552
1610.6448
868.90
1274.30
3 1.2199E3 504.06203 2.91020E2
-32.2262
2472.0928
914.44
1801.73
t2m1
3 2.1562E3 554.19036 3.19962E2
779.5448
3532.9152
1751.19
2787.80
t2m2
3 9.1890E2 456.11008 2.63335E2
-214.1402
2051.9402
439.85
1347.95
t2m3
3 1.7542E3 901.55101 5.20511E2
3993.8269
768.90
2537.80
t2m4
3 1.1037E3 119.19750
68.81871
807.5703
1399.7763
968.90
1195.25
t3m1
3 1.5567E3 313.22895 1.80843E2
778.6295
2334.8372
1195.25
1747.95
t3m2
3 1.3143E3 178.32206 1.02954E2
871.3601
1757.3132
1121.60
1473.46
t3m3
3 1.0117E3 220.48833 1.27299E2
463.9300
1559.3767
786.36
1227.00
t3m4
3 1.4146E3 512.43734 2.95856E2
141.6517
2687.5816
1068.90
2003.35
83.73793 1194.8705
1534.8645
439.85
2787.80
Total
89.82171
bio.unsoed.ac.id
-485.3269
36 1.3649E3 502.42757
73
Multiple Comparisons
Panjang Sirip Caudal
LSD
(I)
(J)
Interak Interak
Mean
si
si
Difference (I-J) Std. Error
t1m1
t1m2
t1m3
Sig.
Lower Bound Upper Bound
t1m2
-487.29333 3.55470E2
.183
-1220.9477
246.3611
t1m3
76.17000 3.55470E2
.832
-657.4844
809.8244
t1m4
-47.61333 3.55470E2
.895
-781.2677
686.0411
*
t2m1
-983.91000 3.55470E2
.011
-1717.5644
-250.2556
t2m2
253.42000 3.55470E2
.483
-480.2344
987.0744
t2m3
-581.93000 3.55470E2
.115
-1315.5844
151.7244
t2m4
68.64667 3.55470E2
.848
-665.0077
802.3011
t3m1
-384.41333 3.55470E2
.290
-1118.0677
349.2411
t3m2
-142.01667 3.55470E2
.693
-875.6711
591.6377
t3m3
160.66667 3.55470E2
.655
-572.9877
894.3211
t3m4
t1m1
-242.29667 3.55470E2
487.29333 3.55470E2
.502
.183
-975.9511
-246.3611
491.3577
1220.9477
t1m3
563.46333 3.55470E2
.126
-170.1911
1297.1177
t1m4
t2m1
439.68000 3.55470E2
-496.61667 3.55470E2
.228
.175
-293.9744
-1230.2711
1173.3344
237.0377
t2m2
740.71333* 3.55470E2
.048
7.0589
1474.3677
t2m3
-94.63667 3.55470E2
.792
-828.2911
639.0177
t2m4
t3m1
555.94000 3.55470E2
102.88000 3.55470E2
.131
.775
-177.7144
-630.7744
1289.5944
836.5344
t3m2
345.27667 3.55470E2
.341
-388.3777
1078.9311
t3m3
647.96000 3.55470E2
.081
-85.6944
1381.6144
t3m4
t1m1
244.99667 3.55470E2
-76.17000 3.55470E2
.497
.832
-488.6577
-809.8244
978.6511
657.4844
t1m2
-563.46333 3.55470E2
.126
-1297.1177
170.1911
t1m4
-123.78333 3.55470E2
.731
-857.4377
609.8711
*
t2m1
-1060.08000 3.55470E2
.006
-1793.7344
-326.4256
t2m2
177.25000 3.55470E2
.623
-556.4044
910.9044
t2m3
-658.10000 3.55470E2
.076
-1391.7544
75.5544
t2m4
t1m4
95% Confidence Interval
bio.unsoed.ac.id
-7.52333 3.55470E2
.983
-741.1777
726.1311
t3m1
-460.58333 3.55470E2
.207
-1194.2377
273.0711
t3m2
-218.18667 3.55470E2
.545
-951.8411
515.4677
t3m3
84.49667 3.55470E2
.814
-649.1577
818.1511
t3m4
-318.46667 3.55470E2
.379
-1052.1211
415.1877
t1m1
t1m2
47.61333 3.55470E2
-439.68000 3.55470E2
.895
.228
-686.0411
-1173.3344
781.2677
293.9744
t1m3
123.78333 3.55470E2
.731
-609.8711
857.4377
73
t2m1
t2m2
t2m3
t2m4
t2m1
-936.29667* 3.55470E2
.015
-1669.9511
-202.6423
t2m2
301.03333 3.55470E2
.405
-432.6211
1034.6877
t2m3
-534.31667 3.55470E2
.146
-1267.9711
199.3377
t2m4
t3m1
116.26000 3.55470E2
-336.80000 3.55470E2
.746
.353
-617.3944
-1070.4544
849.9144
396.8544
t3m2
-94.40333 3.55470E2
.793
-828.0577
639.2511
t3m3
208.28000 3.55470E2
.563
-525.3744
941.9344
t3m4
t1m1
-194.68333 3.55470E2
983.91000* 3.55470E2
.589
.011
-928.3377
250.2556
538.9711
1717.5644
t1m2
496.61667 3.55470E2
.175
-237.0377
1230.2711
t1m3
t1m4
*
1060.08000 3.55470E2
936.29667* 3.55470E2
.006
.015
326.4256
202.6423
1793.7344
1669.9511
t2m2
1237.33000* 3.55470E2
.002
503.6756
1970.9844
t2m3
401.98000 3.55470E2
.269
-331.6744
1135.6344
*
t2m4
t3m1
1052.55667 3.55470E2
599.49667 3.55470E2
.007
.105
318.9023
-134.1577
1786.2111
1333.1511
t3m2
841.89333* 3.55470E2
.026
108.2389
1575.5477
t3m3
1144.57667* 3.55470E2
.004
410.9223
1878.2311
t3m4
*
741.61333 3.55470E2
.048
7.9589
1475.2677
t1m1
-253.42000 3.55470E2
.483
-987.0744
480.2344
*
t1m2
-740.71333 3.55470E2
.048
-1474.3677
-7.0589
t1m3
-177.25000 3.55470E2
.623
-910.9044
556.4044
t1m4
t2m1
-301.03333 3.55470E2
-1237.33000* 3.55470E2
.405
.002
-1034.6877
-1970.9844
432.6211
-503.6756
t2m3
-835.35000* 3.55470E2
.027
-1569.0044
-101.6956
t2m4
-184.77333 3.55470E2
.608
-918.4277
548.8811
t3m1
-637.83333 3.55470E2
.085
-1371.4877
95.8211
t3m2
-395.43667 3.55470E2
.277
-1129.0911
338.2177
t3m3
-92.75333 3.55470E2
.796
-826.4077
640.9011
t3m4
-495.71667 3.55470E2
.176
-1229.3711
237.9377
t1m1
581.93000 3.55470E2
.115
-151.7244
1315.5844
t1m2
94.63667 3.55470E2
.792
-639.0177
828.2911
t1m3
658.10000 3.55470E2
.076
-75.5544
1391.7544
t1m4
534.31667 3.55470E2
.146
-199.3377
1267.9711
t2m1
t2m2
-401.98000 3.55470E2
835.35000* 3.55470E2
.027
101.6956
331.6744
1569.0044
t2m4
650.57667 3.55470E2
.080
-83.0777
1384.2311
t3m1
197.51667 3.55470E2
.584
-536.1377
931.1711
t3m2
439.91333 3.55470E2
.228
-293.7411
1173.5677
bio.unsoed.ac.id
.269
-1135.6344
*
t3m3
742.59667 3.55470E2
.047
8.9423
1476.2511
t3m4
339.63333 3.55470E2
.349
-394.0211
1073.2877
t1m1
-68.64667 3.55470E2
.848
-802.3011
665.0077
73
t3m1
t3m2
t3m3
t1m2
-555.94000 3.55470E2
.131
-1289.5944
177.7144
t1m3
7.52333 3.55470E2
.983
-726.1311
741.1777
t1m4
-116.26000 3.55470E2
.746
-849.9144
617.3944
*
t2m1
t2m2
-1052.55667 3.55470E2
184.77333 3.55470E2
.007
.608
-1786.2111
-548.8811
-318.9023
918.4277
t2m3
-650.57667 3.55470E2
.080
-1384.2311
83.0777
t3m1
-453.06000 3.55470E2
.215
-1186.7144
280.5944
t3m2
t3m3
-210.66333 3.55470E2
92.02000 3.55470E2
.559
.798
-944.3177
-641.6344
522.9911
825.6744
t3m4
-310.94333 3.55470E2
.390
-1044.5977
422.7111
t1m1
t1m2
384.41333 3.55470E2
-102.88000 3.55470E2
.290
.775
-349.2411
-836.5344
1118.0677
630.7744
t1m3
460.58333 3.55470E2
.207
-273.0711
1194.2377
t1m4
336.80000 3.55470E2
.353
-396.8544
1070.4544
t2m1
t2m2
-599.49667 3.55470E2
637.83333 3.55470E2
.105
.085
-1333.1511
-95.8211
134.1577
1371.4877
t2m3
-197.51667 3.55470E2
.584
-931.1711
536.1377
t2m4
453.06000 3.55470E2
.215
-280.5944
1186.7144
t3m2
242.39667 3.55470E2
.502
-491.2577
976.0511
t3m3
545.08000 3.55470E2
.138
-188.5744
1278.7344
t3m4
142.11667 3.55470E2
.693
-591.5377
875.7711
t1m1
142.01667 3.55470E2
.693
-591.6377
875.6711
t1m2
t1m3
-345.27667 3.55470E2
218.18667 3.55470E2
.341
.545
-1078.9311
-515.4677
388.3777
951.8411
t1m4
94.40333 3.55470E2
.793
-639.2511
828.0577
*
t2m1
-841.89333 3.55470E2
.026
-1575.5477
-108.2389
t2m2
395.43667 3.55470E2
.277
-338.2177
1129.0911
t2m3
-439.91333 3.55470E2
.228
-1173.5677
293.7411
t2m4
210.66333 3.55470E2
.559
-522.9911
944.3177
t3m1
-242.39667 3.55470E2
.502
-976.0511
491.2577
t3m3
302.68333 3.55470E2
.403
-430.9711
1036.3377
t3m4
-100.28000 3.55470E2
.780
-833.9344
633.3744
t1m1
-160.66667 3.55470E2
.655
-894.3211
572.9877
t1m2
-647.96000 3.55470E2
.081
-1381.6144
85.6944
bio.unsoed.ac.id
-84.49667 3.55470E2
.814
-818.1511
t1m3
t1m4
-208.28000 3.55470E2
.563
-941.9344
649.1577
525.3744
t2m1
-1144.57667* 3.55470E2
.004
-1878.2311
-410.9223
t2m2
92.75333 3.55470E2
.796
-640.9011
826.4077
*
t2m3
-742.59667 3.55470E2
.047
-1476.2511
-8.9423
t2m4
-92.02000 3.55470E2
.798
-825.6744
641.6344
t3m1
-545.08000 3.55470E2
.138
-1278.7344
188.5744
t3m2
-302.68333 3.55470E2
.403
-1036.3377
430.9711
73
t3m4
-402.96333 3.55470E2
.268
-1136.6177
330.6911
t1m1
242.29667 3.55470E2
.502
-491.3577
975.9511
t1m2
-244.99667 3.55470E2
.497
-978.6511
488.6577
t1m3
t1m4
318.46667 3.55470E2
194.68333 3.55470E2
.379
.589
-415.1877
-538.9711
1052.1211
928.3377
t2m1
-741.61333* 3.55470E2
.048
-1475.2677
-7.9589
t2m2
495.71667 3.55470E2
.176
-237.9377
1229.3711
t2m3
t2m4
-339.63333 3.55470E2
310.94333 3.55470E2
.349
.390
-1073.2877
-422.7111
394.0211
1044.5977
t3m1
-142.11667 3.55470E2
.693
-875.7711
591.5377
t3m2
100.28000 3.55470E2
.780
t3m3
402.96333 3.55470E2
.268
*. The mean difference is significant at the 0.05 level.
-633.3744
-330.6911
833.9344
1136.6177
t3m4
2. Luas Sirip Caudal
Tests of Between-Subjects Effects
Dependent Variable:LUAS SIRIP CAUDAL
Type III Sum
of Squares
Source
Intercept
Suhu
Suhu * Ulangan
Hormon
Suhu * Hormon
Hypothesis
df
Mean Square
2.111E7
1
2.111E7
Error
1175414.280
6
195902.380a
Hypothesis
1220837.642
2
610418.821
a
Error
1175414.280
6
195902.380
Hypothesis
1175414.280
6
195902.380
Error
3336345.851
18
185352.547b
Hypothesis
2407338.413
3
802446.138
b
Error
3336345.851
18
Hypothesis
2797308.143
6
466218.024
Error
3336345.851
18
185352.547b
a. MS(Suhu * Ulangan)
b. MS(Error)
F
bio.unsoed.ac.id
73
Sig.
107.748
.000
3.116
.118
1.057
.423
4.329
.018
2.515
.060
185352.547
Multiple Comparisons
Descriptives
LUAS SIRIP
CAUDAL
Metiltestost
eron
KONTROL
1,6 nM
16 nM
32 nM
Total
Mean
Std.
Deviation
Std. Error
1.1736E3
6.0507E2
7.9179E2
4.9249E2
7.6573E2
595.87269
305.20786
775.91967
126.37564
559.01046
1.98624E2
1.01736E2
2.58640E2
42.12521
93.16841
N
9
9
9
9
36
95% Confidence Interval
for Mean
Lower
Bound
Upper
Bound
715.5361 1631.5927
370.4665 839.6735
195.3620 1388.2113
395.3446 589.6265
576.5847 954.8686
Minimum Maximum
374.99
1.75
124.45
222.34
1.75
2436.05
1081.06
2633.48
670.93
2633.48
LUAS SIRIP CAUDAL
LSD
(I)
(J)
METILTES METILTES
Mean
Std. Error
TOSTERO TOSTERO Difference (I-J)
N
N
95% Confidence Interval
Sig.
Lower Bound Upper Bound
KONTROL 1,6 nM
568.49444* 2.43383E2
.026
72.7388
1064.2501
16 nM
381.77778 2.43383E2
.127
-113.9779
877.5334
*
.009
185.3232
1176.8346
*
-568.49444 2.43383E2
.026
-1064.2501
-72.7388
16 nM
32 nM
-186.71667 2.43383E2
112.58444 2.43383E2
.449
.647
-682.4723
-383.1712
309.0390
608.3401
KONTROL
-381.77778 2.43383E2
.127
-877.5334
113.9779
186.71667 2.43383E2
.449
-309.0390
682.4723
299.30111 2.43383E2
-681.07889* 2.43383E2
.228
.009
-196.4546
-1176.8346
795.0568
-185.3232
-112.58444 2.43383E2
.647
-608.3401
383.1712
16 nM
-299.30111 2.43383E2
*. The mean difference is significant at the 0.05 level.
.228
-795.0568
196.4546
32 nM
1,6 nM
16 nM
681.07889 2.43383E2
KONTROL
1,6 nM
32 nM
32 nM
KONTROL
1,6 nM
bio.unsoed.ac.id
73
Lampiran 4. Hasil analisis ANOVA panjang dan luas sirip dorsal ikan Nilem
(O. hasselti) yang dipelihara dalam medium dengan temperatur
dan kadar metiltestosteron berbeda.
1. Panjang Sirip Dorsal
Tests of Between-Subjects Effects
Dependent Variable:PANJANG SIRIP DORSAL
Type III Sum
of Squares
Source
Intercept
df
Mean Square
Hypothesis
1.450E7
1
1.450E7
Error
Suhu
Hypothesis
Error
Suhu * Ulangan
Hypothesis
Error
Hormon
Hypothesis
Error
Suhu * Hormon
Hypothesis
Error
a. MS(Suhu * Ulangan)
b. MS(Error)
2590796.684
1043037.880
2590796.684
2590796.684
3245759.622
910321.145
3245759.622
148539.152
3245759.622
6
2
6
6
18
3
18
6
18
431799.447a
521518.940
431799.447a
431799.447
180319.979b
303440.382
180319.979b
24756.525
180319.979b
F
Sig.
33.571
.001
1.208
.362
2.395
.071
1.683
.206
.137
.989
2. Luas Sirip Dorsal
Tests of Between-Subjects Effects
Dependent Variable:LUAS SIRIP DORSAL
Type III Sum
of Squares
Source
Intercept
df
Mean Square
Hypothesis
1647188.287
1
1647188.287
Error
Suhu
Hypothesis
Error
Suhu * Ulangan
Hypothesis
Error
Hormon
Hypothesis
Error
Suhu * Hormon
Hypothesis
Error
a. MS(Suhu * Ulangan)
b. MS(Error)
677313.890
407069.857
677313.890
677313.890
355325.747
344106.914
355325.747
651482.341
355325.747
6
2
6
6
18
3
18
6
18
112885.648a
203534.929
112885.648a
112885.648
19740.319b
114702.305
19740.319b
108580.390
19740.319b
bio.unsoed.ac.id
73
F
Sig.
14.592
.009
1.803
.244
5.719
.002
5.811
.006
5.500
.002
Descriptives
LUAS SIRIP DORSAL
95% Confidence Interval
for Mean
Interaksi
t1m1
t1m2
t1m3
t1m4
t2m1
t2m2
t2m3
t2m4
t3m1
t3m2
t3m3
t3m4
Total
N
Mean
Std.
Deviation
Std. Error
Lower
Bound
3
3
3
3
3
3
3
3
3
3
3
3
36
1.4112E2
3.3387E2
47.4067
1.1452E2
7.1115E2
1.0631E2
5.0637E2
1.2644E2
2.3858E2
1.2216E2
87.0733
31.8633
2.1390E2
219.57102
158.44780
51.59707
177.67154
407.17165
151.33297
441.89402
111.57109
72.51268
76.66350
21.53881
27.66039
263.78014
1.26769E2
91.47988
29.78958
1.02579E2
2.35081E2
87.37213
2.55128E2
64.41560
41.86522
44.26169
12.43544
15.96973
43.96336
-404.3213
-59.7361
-80.7676
-326.8406
-300.3204
-269.6253
-591.3589
-150.7213
58.4452
-68.2794
33.5680
-36.8489
124.6544
Upper Bound Minimum Maximum
686.5680
727.4761
175.5809
555.8806
1722.6204
482.2386
1604.0923
403.5946
418.7082
312.6060
140.5787
100.5756
303.1551
4.32
180.67
3.24
9.05
281.21
15.68
76.42
3.24
154.86
76.42
66.42
14.86
3.24
Multiple Comparisons
LUAS SIRIP DORSAL
LSD
(I)
(J)
Mean
INTER INTER Difference
AKSI AKSI
(I-J)
t1m1
95% Confidence Interval
Std. Error
Sig.
Lower Bound
Upper Bound
t1m2
-192.74667 1.69365E2
.266
-542.2984
156.8051
t1m3
93.71667 1.69365E2
.585
-255.8351
443.2684
t1m4
26.60333 1.69365E2
.876
-322.9484
376.1551
t2m1
-570.02667* 1.69365E2
.003
-919.5784
-220.4749
t2m2
34.81667 1.69365E2
.839
-314.7351
bio.unsoed.ac.id
384.3684
t2m3
-365.24333* 1.69365E2
.041
-714.7951
-15.6916
t2m4
14.68667 1.69365E2
.932
-334.8651
364.2384
t3m1
-97.45333 1.69365E2
.570
-447.0051
252.0984
t3m2
18.96000 1.69365E2
.912
-330.5918
368.5118
t3m3
54.05000 1.69365E2
.752
-295.5018
403.6018
t3m4
109.26000 1.69365E2
.525
-240.2918
458.8118
73
394.39
497.09
104.12
319.65
1090.90
281.01
959.31
220.67
281.75
210.67
109.40
63.78
1090.90
t1m2
t1m3
t1m4
t2m1
t1m1
192.74667 1.69365E2
.266
-156.8051
542.2984
t1m3
286.46333 1.69365E2
.104
-63.0884
636.0151
t1m4
219.35000 1.69365E2
.208
-130.2018
568.9018
t2m1
-377.28000* 1.69365E2
.036
-726.8318
-27.7282
t2m2
227.56333 1.69365E2
.192
-121.9884
577.1151
t2m3
-172.49667 1.69365E2
.319
-522.0484
177.0551
t2m4
207.43333 1.69365E2
.233
-142.1184
556.9851
t3m1
95.29333 1.69365E2
.579
-254.2584
444.8451
t3m2
211.70667 1.69365E2
.223
-137.8451
561.2584
t3m3
246.79667 1.69365E2
.158
-102.7551
596.3484
t3m4
302.00667 1.69365E2
.087
-47.5451
651.5584
t1m1
-93.71667 1.69365E2
.585
-443.2684
255.8351
t1m2
-286.46333 1.69365E2
.104
-636.0151
63.0884
t1m4
-67.11333 1.69365E2
.695
-416.6651
282.4384
t2m1
-663.74333* 1.69365E2
.001
-1013.2951
-314.1916
t2m2
-58.90000 1.69365E2
.731
-408.4518
290.6518
t2m3
-458.96000* 1.69365E2
.012
-808.5118
-109.4082
t2m4
-79.03000 1.69365E2
.645
-428.5818
270.5218
t3m1
-191.17000 1.69365E2
.270
-540.7218
158.3818
t3m2
-74.75667 1.69365E2
.663
-424.3084
274.7951
t3m3
-39.66667 1.69365E2
.817
-389.2184
309.8851
t3m4
15.54333 1.69365E2
.928
-334.0084
365.0951
t1m1
-26.60333 1.69365E2
.876
-376.1551
322.9484
t1m2
-219.35000 1.69365E2
.208
-568.9018
130.2018
t1m3
67.11333 1.69365E2
.695
-282.4384
416.6651
t2m1
-596.63000* 1.69365E2
.002
-946.1818
-247.0782
t2m2
8.21333 1.69365E2
.962
-341.3384
357.7651
t2m3
-391.84667* 1.69365E2
.030
-741.3984
-42.2949
t2m4
-11.91667 1.69365E2
t3m1
-124.05667 1.69365E2
.471
-473.6084
225.4951
t3m2
-7.64333 1.69365E2
.964
-357.1951
341.9084
t3m3
27.44667 1.69365E2
.873
-322.1051
376.9984
t3m4
82.65667 1.69365E2
.630
-266.8951
432.2084
t1m1
570.02667* 1.69365E2
.003
220.4749
919.5784
t1m2
377.28000* 1.69365E2
.036
27.7282
726.8318
bio.unsoed.ac.id
.944
-361.4684
73
337.6351
t2m2
t2m3
t2m4
t1m3
663.74333* 1.69365E2
.001
314.1916
1013.2951
t1m4
596.63000* 1.69365E2
.002
247.0782
946.1818
t2m2
604.84333* 1.69365E2
.002
255.2916
954.3951
t2m3
204.78333 1.69365E2
.238
-144.7684
554.3351
t2m4
584.71333* 1.69365E2
.002
235.1616
934.2651
t3m1
472.57333* 1.69365E2
.010
123.0216
822.1251
t3m2
588.98667* 1.69365E2
.002
239.4349
938.5384
t3m3
624.07667* 1.69365E2
.001
274.5249
973.6284
t3m4
679.28667* 1.69365E2
.001
329.7349
1028.8384
t1m1
-34.81667 1.69365E2
.839
-384.3684
314.7351
t1m2
-227.56333 1.69365E2
.192
-577.1151
121.9884
t1m3
58.90000 1.69365E2
.731
-290.6518
408.4518
t1m4
-8.21333 1.69365E2
.962
-357.7651
341.3384
t2m1
-604.84333* 1.69365E2
.002
-954.3951
-255.2916
t2m3
-400.06000* 1.69365E2
.027
-749.6118
-50.5082
t2m4
-20.13000 1.69365E2
.906
-369.6818
329.4218
t3m1
-132.27000 1.69365E2
.442
-481.8218
217.2818
t3m2
-15.85667 1.69365E2
.926
-365.4084
333.6951
t3m3
19.23333 1.69365E2
.911
-330.3184
368.7851
t3m4
74.44333 1.69365E2
.664
-275.1084
423.9951
t1m1
365.24333* 1.69365E2
.041
15.6916
714.7951
t1m2
172.49667 1.69365E2
.319
-177.0551
522.0484
t1m3
458.96000* 1.69365E2
.012
109.4082
808.5118
t1m4
391.84667* 1.69365E2
.030
42.2949
741.3984
t2m1
-204.78333 1.69365E2
.238
-554.3351
144.7684
t2m2
400.06000* 1.69365E2
.027
50.5082
749.6118
t2m4
379.93000* 1.69365E2
.034
30.3782
729.4818
t3m1
267.79000 1.69365E2
.127
-81.7618
617.3418
t3m2
384.20333* 1.69365E2
t3m3
419.29333* 1.69365E2
.021
69.7416
768.8451
t3m4
474.50333* 1.69365E2
.010
124.9516
824.0551
t1m1
-14.68667 1.69365E2
.932
-364.2384
334.8651
t1m2
-207.43333 1.69365E2
.233
-556.9851
142.1184
t1m3
79.03000 1.69365E2
.645
-270.5218
428.5818
t1m4
11.91667 1.69365E2
.944
-337.6351
361.4684
bio.unsoed.ac.id
.033
34.6516
73
733.7551
t3m1
t3m2
t3m3
t2m1
-584.71333* 1.69365E2
.002
-934.2651
-235.1616
t2m2
20.13000 1.69365E2
.906
-329.4218
369.6818
t2m3
-379.93000* 1.69365E2
.034
-729.4818
-30.3782
t3m1
-112.14000 1.69365E2
.514
-461.6918
237.4118
t3m2
4.27333 1.69365E2
.980
-345.2784
353.8251
t3m3
39.36333 1.69365E2
.818
-310.1884
388.9151
t3m4
94.57333 1.69365E2
.582
-254.9784
444.1251
t1m1
97.45333 1.69365E2
.570
-252.0984
447.0051
t1m2
-95.29333 1.69365E2
.579
-444.8451
254.2584
t1m3
191.17000 1.69365E2
.270
-158.3818
540.7218
t1m4
124.05667 1.69365E2
.471
-225.4951
473.6084
t2m1
-472.57333* 1.69365E2
.010
-822.1251
-123.0216
t2m2
132.27000 1.69365E2
.442
-217.2818
481.8218
t2m3
-267.79000 1.69365E2
.127
-617.3418
81.7618
t2m4
112.14000 1.69365E2
.514
-237.4118
461.6918
t3m2
116.41333 1.69365E2
.498
-233.1384
465.9651
t3m3
151.50333 1.69365E2
.380
-198.0484
501.0551
t3m4
206.71333 1.69365E2
.234
-142.8384
556.2651
t1m1
-18.96000 1.69365E2
.912
-368.5118
330.5918
t1m2
-211.70667 1.69365E2
.223
-561.2584
137.8451
t1m3
74.75667 1.69365E2
.663
-274.7951
424.3084
t1m4
7.64333 1.69365E2
.964
-341.9084
357.1951
t2m1
-588.98667* 1.69365E2
.002
-938.5384
-239.4349
t2m2
15.85667 1.69365E2
.926
-333.6951
365.4084
t2m3
-384.20333* 1.69365E2
.033
-733.7551
-34.6516
t2m4
-4.27333 1.69365E2
.980
-353.8251
345.2784
t3m1
-116.41333 1.69365E2
.498
-465.9651
233.1384
t3m3
35.09000 1.69365E2
.838
-314.4618
384.6418
t3m4
90.30000 1.69365E2
t1m1
-54.05000 1.69365E2
.752
-403.6018
295.5018
t1m2
-246.79667 1.69365E2
.158
-596.3484
102.7551
t1m3
39.66667 1.69365E2
.817
-309.8851
389.2184
t1m4
-27.44667 1.69365E2
.873
-376.9984
322.1051
t2m1
-624.07667* 1.69365E2
.001
-973.6284
-274.5249
t2m2
-19.23333 1.69365E2
.911
-368.7851
330.3184
bio.unsoed.ac.id
.599
-259.2518
73
439.8518
t3m4
t2m3
-419.29333* 1.69365E2
.021
-768.8451
-69.7416
t2m4
-39.36333 1.69365E2
.818
-388.9151
310.1884
t3m1
-151.50333 1.69365E2
.380
-501.0551
198.0484
t3m2
-35.09000 1.69365E2
.838
-384.6418
314.4618
t3m4
55.21000 1.69365E2
.747
-294.3418
404.7618
t1m1
-109.26000 1.69365E2
.525
-458.8118
240.2918
t1m2
-302.00667 1.69365E2
.087
-651.5584
47.5451
t1m3
-15.54333 1.69365E2
.928
-365.0951
334.0084
t1m4
-82.65667 1.69365E2
.630
-432.2084
266.8951
t2m1
-679.28667* 1.69365E2
.001
-1028.8384
-329.7349
t2m2
-74.44333 1.69365E2
.664
-423.9951
275.1084
t2m3
-474.50333* 1.69365E2
.010
-824.0551
-124.9516
t2m4
-94.57333 1.69365E2
.582
-444.1251
254.9784
t3m1
-206.71333 1.69365E2
.234
-556.2651
142.8384
t3m2
-90.30000 1.69365E2
.599
-439.8518
259.2518
t3m3
-55.21000 1.69365E2
.747
-404.7618
294.3418
*. The mean difference is significant at the 0.05 level.
bio.unsoed.ac.id
73
Lampiran 5. Hasil analisis ANOVA panjang dan luas sirip pectoral ikan Nilem
(O. hasselti) yang dipelihara dalam medium dengan temperatur
dan kadar metiltestosteron berbeda.
1. Panjang Sirip Pectoral
Tests of Between-Subjects Effects
Dependent Variable:PANJANG SIRIP
PECTORAL
Type III Sum
of Squares
Source
Intercept
Hypothesis
Error
Suhu
Hypothesis
Error
Suhu * Ulangan
Hypothesis
Error
Hormon
Hypothesis
Error
Suhu * Hormon
Hypothesis
Error
a. MS(Suhu * Ulangan)
b. MS(Error)
df
3.302E7
235660.396
160167.880
235660.396
235660.396
1205159.976
157692.190
1205159.976
264515.166
1205159.976
Mean Square
F
1
3.302E7
6
2
6
6
18
3
18
6
18
a
39276.733
80083.940
39276.733a
39276.733
66953.332b
52564.063
66953.332b
44085.861
66953.332b
Sig.
840.711
.000
2.039
.211
.587
.737
.785
.518
.658
.684
2. Luas Sirip Pectoral
Tests of Between-Subjects Effects
Dependent Variable:LUAS SIRIP PECTORAL
Type III Sum
of Squares
Source
Intercept
Hypothesis
Error
Suhu
Hypothesis
Error
Suhu * Ulangan
Hypothesis
Error
Hormon
Hypothesis
Error
Suhu * Hormon
Hypothesis
Error
a. MS(Suhu * Ulangan)
b. MS(Error)
6334122.844
178644.242
23865.812
178644.242
178644.242
336287.995
137784.693
336287.995
181570.797
336287.995
df
Mean Square
1
6334122.844
6
2
6
6
18
3
18
6
18
a
bio.unsoed.ac.id
73
F
29774.040
11932.906
29774.040a
29774.040
18682.666b
45928.231
18682.666b
30261.800
18682.666b
Sig.
212.740
.000
.401
.686
1.594
.206
2.458
.096
1.620
.199
Lampiran 6. Hasil analisis ANOVA panjang dan luas sirip anal ikan Nilem
(O. hasselti) yang dipelihara dalam medium dengan temperatur
dan kadar metiltestosteron berbeda.
1. Panjang Sirip Anal
Tests of Between-Subjects Effects
Dependent Variable:PANJANG SIRIP ANAL
Type III Sum
of Squares
Source
Intercept
Hypothesis
Error
Suhu
Hypothesis
Error
Suhu * Ulangan
Hypothesis
Error
Hormon
Hypothesis
Error
Suhu * Hormon
Hypothesis
Error
a. MS(Suhu * Ulangan)
b. MS(Error)
df
Mean Square
1.283E7
522773.348
208797.072
522773.348
522773.348
1374520.002
1099169.873
1374520.002
1104762.639
1374520.002
F
1
1.283E7
6
2
6
6
18
3
18
6
18
a
Sig.
147.247
.000
1.198
.365
1.141
.379
4.798
.013
2.411
.069
Minimum
Maximum
87128.891
104398.536
87128.891a
87128.891
76362.222b
366389.958
76362.222b
184127.107
76362.222b
Descriptives
PANJANG SIRIP
ANAL
Metil
testosteron
KONTROL
1,6 nM
16 nM
32 nM
Total
N
9
9
9
9
36
Mean
8.8416E2
5.0951E2
5.7475E2
4.1946E2
5.9697E2
Std.
Deviation
Std. Error
380.16387 1.26721E2
214.15475 71.38492
360.38300 1.20128E2
284.76991 94.92330
350.91810 58.48635
95% Confidence
Interval for Mean
Lower
Bound
591.9402 1176.3798
344.8950 674.1228
297.7329 851.7627
200.5698 638.3569
478.2364 715.7036
bio.unsoed.ac.id
73
Upper
Bound
150.00
161.75
168.10
68.10
68.10
1421.60
837.15
1421.60
866.20
1421.60
Multiple Comparisons
PANJANG SIRIP ANAL
LSD
(I)
(J)
METILTES METILTES
Mean
Std. Error
TOSTERO TOSTERO Difference (I-J)
N
N
95% Confidence Interval
Sig.
374.65111* 1.49324E2
KONTROL 1,6 nM
*
Lower Bound Upper Bound
.017
70.4885
678.8137
16 nM
309.41222 1.49324E2
.046
5.2496
613.5748
32 nM
464.69667* 1.49324E2
.004
160.5341
768.8592
-374.65111 1.49324E2
.017
-678.8137
-70.4885
16 nM
-65.23889 1.49324E2
.665
-369.4015
238.9237
32 nM
90.04556 1.49324E2
.551
-214.1170
394.2081
-309.41222 1.49324E2
.046
-613.5748
-5.2496
1,6 nM
65.23889 1.49324E2
.665
-238.9237
369.4015
32 nM
155.28444 1.49324E2
.306
-148.8781
459.4470
-464.69667 1.49324E2
-90.04556 1.49324E2
.004
.551
-768.8592
-394.2081
-160.5341
214.1170
16 nM
-155.28444 1.49324E2
*. The mean difference is significant at the 0.05 level.
.306
-459.4470
148.8781
1,6 nM
16 nM
32 nM
KONTROL
KONTROL
KONTROL
1,6 nM
*
*
*
2. Luas Sirip Anal
Tests of Between-Subjects Effects
Dependent Variable:LUAS SIRIP ANAL
Type III Sum
of Squares
Source
Intercept
Suhu
Suhu * Ulangan
Hormon
Suhu * Hormon
Hypothesis
Mean Square
F
1
277623.610
6
2
6
6
18
3
18
6
a
9601.297
16079.496
9601.297a
9601.297
2647.986b
16324.507
2647.986b
11295.988
47663.751
18
bio.unsoed.ac.id
2647.986b
Error
Hypothesis
Error
Hypothesis
Error
Hypothesis
Error
Hypothesis
Error
277623.610
df
57607.783
32158.991
57607.783
57607.783
47663.751
48973.522
47663.751
67775.931
a. MS(Suhu * Ulangan)
b. MS(Error)
73
Sig.
28.915
.002
1.675
.264
3.626
.016
6.165
.005
4.266
.008
Descriptives
Luas Sirip Anal
95% Confidence Interval
for Mean
Interaksi
N
t1m1
t1m2
t1m3
t1m4
t2m1
t2m2
t2m3
t2m4
t3m1
t3m2
t3m3
t3m4
Total
3
3
3
3
3
3
3
3
3
3
3
3
36
Mean
Std.
Deviation Std. Error Lower Bound Upper Bound Minimum Maximum
42.3467 28.27991 16.32741
1.2628E2 45.16820 26.07787
43.1533
3.18957 1.84150
38.9433 15.60585 9.01004
2.4781E2 104.98236 60.61160
49.9100 26.63805 15.37949
1.5797E2 142.16000 82.07611
63.5667 77.30838 44.63402
1.3891E2 50.00094 28.86805
38.1533 23.85924 13.77514
82.6333 87.14720 50.31446
24.1200 31.00330 17.89977
87.8167 85.21904 14.20317
-27.9045
14.0760
35.2300
.1762
-12.9807
-16.2626
-195.1750
-128.4780
14.7041
-21.1163
-133.8523
-52.8965
58.9827
112.5978
238.4840
51.0767
77.7104
508.6007
116.0826
511.1150
255.6113
263.1225
97.4230
299.1190
101.1365
116.6506
25.75
90.06
41.02
21.02
152.16
21.29
15.81
6.83
101.08
13.92
21.55
3.65
3.65
Multiple Comparisons
Luas Sirip Anal
LSD
(I)
(J)
INTER INTER
Mean
AKSI AKSI Difference (I-J) Std. Error
t1m1
Sig.
Lower Bound Upper Bound
t1m2
-83.93333
54.07596
.134
-195.5406
27.6740
t1m3
-.80667
54.07596
.988
-112.4140
110.8006
t1m4
3.40333
54.07596
.950
-108.2040
115.0106
t2m1
*
54.07596
.001
-317.0706
-93.8560
t2m2
-7.56333
54.07596
.890
-119.1706
104.0440
t2m3
*
54.07596
.043
-227.2306
-4.0160
t2m4
-21.22000
54.07596
.698
-132.8273
90.3873
t3m1
-96.56667
54.07596
.087
-208.1740
15.0406
t3m2
4.19333
54.07596
.939
-107.4140
115.8006
t3m3
-40.28667
54.07596
.464
-151.8940
71.3206
t3m4
t1m2
95% Confidence Interval
-205.46333
-115.62333
bio.unsoed.ac.id
18.22667 54.07596
.739
-93.3806
129.8340
t1m1
83.93333
54.07596
.134
-27.6740
195.5406
t1m3
83.12667
54.07596
.137
-28.4806
194.7340
t1m4
t2m1
87.33667
-121.53000*
54.07596
54.07596
.119
.034
-24.2706
-233.1373
198.9440
-9.9227
t2m2
76.37000
54.07596
.171
-35.2373
187.9773
t2m3
-31.69000
54.07596
.563
-143.2973
79.9173
t2m4
62.71333
54.07596
.258
-48.8940
174.3206
73
75.00
176.89
46.82
49.52
360.13
73.98
300.13
151.62
195.60
61.62
182.43
59.79
360.13
t1m3
t1m4
t2m1
t3m1
-12.63333
54.07596
.817
-124.2406
98.9740
t3m2
88.12667
54.07596
.116
-23.4806
199.7340
t3m3
43.64667
54.07596
.428
-67.9606
155.2540
t3m4
t1m1
102.16000
.80667
54.07596
54.07596
.071
.988
-9.4473
-110.8006
213.7673
112.4140
t1m2
-83.12667
54.07596
.137
-194.7340
28.4806
t1m4
4.21000
54.07596
.939
-107.3973
115.8173
t2m1
t2m2
*
-204.65667
-6.75667
54.07596
54.07596
.001
.902
-316.2640
-118.3640
-93.0494
104.8506
t2m3
-114.81667*
54.07596
.044
-226.4240
-3.2094
t2m4
t3m1
-20.41333
-95.76000
54.07596
54.07596
.709
.089
-132.0206
-207.3673
91.1940
15.8473
t3m2
5.00000
54.07596
.927
-106.6073
116.6073
t3m3
-39.48000
54.07596
.472
-151.0873
72.1273
t3m4
t1m1
19.03333
-3.40333
54.07596
54.07596
.728
.950
-92.5740
-115.0106
130.6406
108.2040
t1m2
-87.33667
54.07596
.119
-198.9440
24.2706
t1m3
-4.21000
54.07596
.939
-115.8173
107.3973
t2m1
*
54.07596
.001
-320.4740
-97.2594
t2m2
-10.96667
54.07596
.841
-122.5740
100.6406
t2m3
*
54.07596
.038
-230.6340
-7.4194
t2m4
-24.62333
54.07596
.653
-136.2306
86.9840
t3m1
t3m2
-99.97000
.79000
54.07596
54.07596
.077
.988
-211.5773
-110.8173
11.6373
112.3973
t3m3
-43.69000
54.07596
.427
-155.2973
67.9173
t3m4
14.82333
54.07596
.786
-96.7840
126.4306
t1m1
*
54.07596
.001
93.8560
317.0706
*
54.07596
.034
9.9227
233.1373
t1m3
*
204.65667
54.07596
.001
93.0494
316.2640
t1m4
208.86667*
54.07596
.001
97.2594
320.4740
t2m2
*
54.07596
.001
86.2927
309.5073
t2m3
89.84000
54.07596
.110
-21.7673
201.4473
t2m4
*
54.07596
.002
72.6360
295.8506
t3m1
108.89667
54.07596
.055
-2.7106
220.5040
t3m2
t3m3
*
209.65667
165.17667*
54.07596
.005
53.5694
321.2640
276.7840
t3m4
223.69000*
54.07596
.000
112.0827
335.2973
t1m1
7.56333
54.07596
.890
-104.0440
119.1706
t1m2
-76.37000
54.07596
.171
-187.9773
35.2373
t1m3
6.75667
54.07596
.902
-104.8506
118.3640
t1m4
10.96667
54.07596
.841
-100.6406
122.5740
t2m1
*
54.07596
.001
-309.5073
-86.2927
t1m2
t2m2
-208.86667
-119.02667
205.46333
121.53000
197.90000
184.24333
bio.unsoed.ac.id
54.07596
.001
98.0494
-197.90000
73
t2m3
t2m4
t3m1
t2m3
-108.06000
54.07596
.057
-219.6673
3.5473
t2m4
-13.65667
54.07596
.803
-125.2640
97.9506
t3m1
-89.00333
54.07596
.113
-200.6106
22.6040
t3m2
t3m3
11.75667
-32.72333
54.07596
54.07596
.830
.551
-99.8506
-144.3306
123.3640
78.8840
t3m4
25.79000
54.07596
.638
-85.8173
137.3973
t1m1
*
115.62333
54.07596
.043
4.0160
227.2306
t1m2
t1m3
31.69000
114.81667*
54.07596
54.07596
.563
.044
-79.9173
3.2094
143.2973
226.4240
t1m4
119.02667*
54.07596
.038
7.4194
230.6340
t2m1
t2m2
-89.84000
108.06000
54.07596
54.07596
.110
.057
-201.4473
-3.5473
21.7673
219.6673
t2m4
94.40333
54.07596
.094
-17.2040
206.0106
t3m1
19.05667
54.07596
.728
-92.5506
130.6640
t3m2
t3m3
*
119.81667
75.33667
54.07596
54.07596
.036
.176
8.2094
-36.2706
231.4240
186.9440
t3m4
133.85000*
54.07596
.021
22.2427
245.4573
t1m1
21.22000
54.07596
.698
-90.3873
132.8273
t1m2
-62.71333
54.07596
.258
-174.3206
48.8940
t1m3
20.41333
54.07596
.709
-91.1940
132.0206
t1m4
24.62333
54.07596
.653
-86.9840
136.2306
t2m1
*
54.07596
.002
-295.8506
-72.6360
t2m2
t2m3
13.65667
-94.40333
54.07596
54.07596
.803
.094
-97.9506
-206.0106
125.2640
17.2040
t3m1
-75.34667
54.07596
.176
-186.9540
36.2606
t3m2
25.41333
54.07596
.643
-86.1940
137.0206
t3m3
-19.06667
54.07596
.727
-130.6740
92.5406
t3m4
39.44667
54.07596
.473
-72.1606
151.0540
t1m1
96.56667
54.07596
.087
-15.0406
208.1740
t1m2
12.63333
54.07596
.817
-98.9740
124.2406
t1m3
95.76000
54.07596
.089
-15.8473
207.3673
t1m4
99.97000
54.07596
.077
-11.6373
211.5773
t2m1
-108.89667
54.07596
.055
-220.5040
2.7106
t2m2
89.00333
54.07596
.113
-22.6040
200.6106
t2m3
t2m4
t3m2
-184.24333
bio.unsoed.ac.id
-19.05667 54.07596
.728
-130.6640
75.34667
54.07596
.176
-36.2606
92.5506
186.9540
t3m2
100.76000
54.07596
.075
-10.8473
212.3673
t3m3
56.28000
54.07596
.308
-55.3273
167.8873
t3m4
*
54.07596
.044
3.1860
226.4006
t1m1
-4.19333
54.07596
.939
-115.8006
107.4140
t1m2
-88.12667
54.07596
.116
-199.7340
23.4806
t1m3
-5.00000
54.07596
.927
-116.6073
106.6073
114.79333
73
t1m4
-.79000
54.07596
.988
-112.3973
110.8173
t2m1
*
54.07596
.001
-321.2640
-98.0494
t2m2
-11.75667
54.07596
.830
-123.3640
99.8506
t2m3
t2m4
*
-119.81667
-25.41333
54.07596
54.07596
.036
.643
-231.4240
-137.0206
-8.2094
86.1940
t3m1
-100.76000
54.07596
.075
-212.3673
10.8473
t3m3
-44.48000
54.07596
.419
-156.0873
67.1273
t3m4
t1m1
14.03333
40.28667
54.07596
54.07596
.797
.464
-97.5740
-71.3206
125.6406
151.8940
t1m2
-43.64667
54.07596
.428
-155.2540
67.9606
t1m3
t1m4
39.48000
43.69000
54.07596
54.07596
.472
.427
-72.1273
-67.9173
151.0873
155.2973
t2m1
-165.17667*
54.07596
.005
-276.7840
-53.5694
t2m2
32.72333
54.07596
.551
-78.8840
144.3306
t2m3
t2m4
-75.33667
19.06667
54.07596
54.07596
.176
.727
-186.9440
-92.5406
36.2706
130.6740
t3m1
-56.28000
54.07596
.308
-167.8873
55.3273
t3m2
44.48000
54.07596
.419
-67.1273
156.0873
t3m4
58.51333
54.07596
.290
-53.0940
170.1206
t1m1
-18.22667
54.07596
.739
-129.8340
93.3806
t1m2
-102.16000
54.07596
.071
-213.7673
9.4473
t1m3
-19.03333
54.07596
.728
-130.6406
92.5740
t1m4
t2m1
-14.82333
-223.69000*
54.07596
54.07596
.786
.000
-126.4306
-335.2973
96.7840
-112.0827
t2m2
-25.79000
54.07596
.638
-137.3973
85.8173
t2m3
*
54.07596
.021
-245.4573
-22.2427
t2m4
-39.44667
54.07596
.473
-151.0540
72.1606
t3m1
*
54.07596
.044
-226.4006
-3.1860
-14.03333
54.07596
.797
-125.6406
97.5740
t3m3
-58.51333 54.07596
.290
*. The mean difference is significant at the 0.05 level.
-170.1206
53.0940
t3m3
t3m4
t3m2
-209.65667
-133.85000
-114.79333
bio.unsoed.ac.id
73
Lampiran 7. Hasil analisis ANOVA panjang dan luas sirip abdominal ikan
Nilem (O. hasselti) yang dipelihara dalam medium dengan
temperatur dan kadar metiltestosteron berbeda.
1. Panjang Sirip Abdominal
Tests of Between-Subjects Effects
Dependent Variable:PANJANG SIRIP ABDOMINAL
Type III Sum
of Squares
Source
Intercept
Hypothesis
df
3.035E7
Error
Suhu
Hypothesis
Error
Suhu * Ulangan
Hypothesis
Error
Hormon
Hypothesis
Error
Suhu * Hormon
Hypothesis
Error
a. MS(Suhu * Ulangan)
b. MS(Error)
1224357.107
3807916.632
1224357.107
1224357.107
3391247.814
547611.738
3391247.814
705800.436
3391247.814
Mean Square
F
1
3.035E7
6
2
6
6
18
3
18
6
18
a
204059.518
1903958.316
204059.518a
204059.518
188402.656b
182537.246
188402.656b
117633.406
188402.656b
Sig.
148.719
.000
9.330
.014
1.083
.409
.969
.429
.624
.709
Descriptives
PANJANG SIRIP ABDOMINAL
95% Confidence
Interval for Mean
Temperat
ur
RUANG
27 C
30 C
Total
N
Mean
12
12
12
36
7.3494E2
1.3751E3
6.4438E2
9.1814E2
Std.
Deviation
Std. Error
Lower
Bound
Upper
Bound
483.85867 1.39678E2 427.5067 1042.3650
500.37365 1.44445E2 1057.1869 1693.0314
221.48140 63.93617 503.6624 785.1076
525.81729 87.63621 740.2324 1096.0543
Minimum Maximum
303.24
657.02
277.97
277.97
2021.60
2274.30
985.53
2274.30
2. Luas Sirip Abdominal
Tests of Between-Subjects Effects
Dependent Variable:LUAS SIRIP ABDOMINAL
Source
Intercept
Suhu
Suhu * Ulangan
Hormon
Type III Sum
of Squares
df
Mean Square
bio.unsoed.ac.id
Hypothesis
210752.151
1
210752.151
Error
Hypothesis
Error
Hypothesis
Error
Hypothesis
Error
101504.462
50137.835
101504.462
101504.462
146046.438
29179.143
146046.438
73
6
2
6
6
18
3
18
16917.410a
25068.917
16917.410a
16917.410
8113.691b
9726.381
8113.691b
F
Sig.
12.458
.012
1.482
.300
2.085
.106
1.199
.339
Suhu * Hormon
Hypothesis
Error
a. MS(Suhu * Ulangan)
b. MS(Error)
93295.429
146046.438
6
18
bio.unsoed.ac.id
73
15549.238
8113.691b
1.916
.133
Lampiran 8. Hasil analisis ANOVA panjang tubuh ikan Nilem (O. hasselti)
yang dipelihara dalam medium dengan temperatur dan kadar
metiltestosteron berbeda.
Tests of Between-Subjects Effects
Dependent Variable:PANJANG TUBUH
Type III Sum of
Squares
Source
Intercept
Hypothesis
Error
Hypothesis
Error
Hypothesis
Error
Hypothesis
Error
Hypothesis
Error
Suhu
Suhu * ulangan
Hormon
Suhu * hormon
df
1854.738
21.396
10.089
21.396
21.396
47.444
.616
47.444
69.444
47.444
Mean Square
F
1
1854.738
6
2
6
6
18
3
18
6
18
a
3.566
5.044
3.566a
3.566
2.636b
.205
2.636b
11.574
2.636b
Sig.
520.108
.000
1.415
.314
1.353
.286
.078
.971
4.391
.007
a. MS(suhu * ulangan)
b. MS(Error)
Descriptives
Panjang Tubuh
Interaksi
Perlakuan
N
Mean
Std.
Deviation
Std.
Error
95% Confidence Interval
for Mean
Lower
Bound
Minimum Maximum
Upper Bound
t1m1
3
6.3500
1.05825
.61098
3.7212
8.9788
5.13
7.02
t1m2
3
7.3900
1.58767
.91664
3.4460
11.3340
6.02
9.13
t1m3
3
6.1267
1.01908
.58837
3.5951
8.6582
5.24
7.24
t1m4
3
6.9333
1.39543
.80565
3.4669
10.3998
5.45
8.22
t2m1
3
9.5733
2.05627 1.18719
4.4653
14.6814
7.56
11.67
t2m2
3
4.3700
.38314
2.7215
6.0185
3.67
4.99
t2m3
3
7.6267
3.14518 1.81587
-.1864
15.4397
4.22
10.42
t2m4
3
6.1000
2.05939 1.18899
.9842
11.2158
4.61
8.45
t3m1
3
5.6800
9.3905
4.42
7.33
t3m2
3
9.4600
1.19578
.69039
6.4895
12.4305
8.24
10.63
t3m3
3
8.3933
2.30622 1.33149
2.6644
14.1223
5.90
10.45
t3m4
3
8.1300
.11000
.06351
7.8567
8.4033
8.02
8.24
Total
36
7.1778
2.06321
.34387
6.4797
7.8759
3.67
11.67
.66363
bio.unsoed.ac.id
1.49369 .86238
1.9695
73
Multiple Comparisons
Panjang Tubuh
LSD
(I)
Interaksi
t1m1
t1m2
t1m3
(J)
Interaksi
95% Confidence Interval
Std. Error
Sig.
Lower Bound
Upper Bound
t1m2
-1.04000
1.38283
.459
-3.8940
1.8140
t1m3
.22333
1.38283
.873
-2.6307
3.0774
t1m4
-.58333
1.38283
.677
-3.4374
2.2707
t2m1
*
1.38283
.028
-6.0774
-.3693
t2m2
1.98000
1.38283
.165
-.8740
4.8340
t2m3
-1.27667
1.38283
.365
-4.1307
1.5774
t2m4
.25000
1.38283
.858
-2.6040
3.1040
t3m1
.67000
1.38283
.632
-2.1840
3.5240
t3m2
*
1.38283
.034
-5.9640
-.2560
t3m3
-2.04333
1.38283
.153
-4.8974
.8107
t3m4
t1m1
-1.78000
1.04000
1.38283
1.38283
.210
.459
-4.6340
-1.8140
1.0740
3.8940
t1m3
1.26333
1.38283
.370
-1.5907
4.1174
t1m4
t2m1
.45667
-2.18333
1.38283
1.38283
.744
.127
-2.3974
-5.0374
3.3107
.6707
t2m2
3.02000*
1.38283
.039
.1660
5.8740
t2m3
-.23667
1.38283
.866
-3.0907
2.6174
t2m4
t3m1
1.29000
1.71000
1.38283
1.38283
.360
.228
-1.5640
-1.1440
4.1440
4.5640
t3m2
-2.07000
1.38283
.147
-4.9240
.7840
t3m3
-1.00333
1.38283
.475
-3.8574
1.8507
t3m4
t1m1
-.74000
-.22333
1.38283
1.38283
.597
.873
-3.5940
-3.0774
2.1140
2.6307
t1m2
-1.26333
1.38283
.370
-4.1174
1.5907
t1m4
-.80667
1.38283
.565
-3.6607
2.0474
t2m1
*
1.38283
.020
-6.3007
-.5926
t2m2
1.75667
1.38283
.216
-1.0974
4.6107
t2m3
-1.50000
1.38283
.289
-4.3540
1.3540
-2.8274
2.8807
t2m4
t1m4
Mean
Difference (IJ)
-3.22333
-3.11000
-3.44667
bio.unsoed.ac.id
.02667
1.38283
.985
t3m1
.44667
1.38283
.749
-2.4074
3.3007
t3m2
*
1.38283
.024
-6.1874
-.4793
t3m3
-2.26667
1.38283
.114
-5.1207
.5874
t3m4
-2.00333
1.38283
.160
-4.8574
.8507
t1m1
t1m2
.58333
-.45667
1.38283
1.38283
.677
.744
-2.2707
-3.3107
3.4374
2.3974
t1m3
.80667
1.38283
.565
-2.0474
3.6607
-3.33333
73
t2m1
t2m2
t2m3
t2m1
-2.64000
1.38283
.068
-5.4940
.2140
t2m2
2.56333
1.38283
.076
-.2907
5.4174
t2m3
-.69333
1.38283
.621
-3.5474
2.1607
t2m4
t3m1
.83333
1.25333
1.38283
1.38283
.552
.374
-2.0207
-1.6007
3.6874
4.1074
t3m2
-2.52667
1.38283
.080
-5.3807
.3274
t3m3
-1.46000
1.38283
.302
-4.3140
1.3940
t3m4
t1m1
-1.19667
3.22333*
1.38283
1.38283
.395
.028
-4.0507
.3693
1.6574
6.0774
t1m2
2.18333
1.38283
.127
-.6707
5.0374
t1m3
t1m4
*
3.44667
2.64000
1.38283
1.38283
.020
.068
.5926
-.2140
6.3007
5.4940
t2m2
5.20333*
1.38283
.001
2.3493
8.0574
t2m3
1.94667
1.38283
.172
-.9074
4.8007
t2m4
t3m1
*
3.47333
3.89333*
1.38283
1.38283
.019
.010
.6193
1.0393
6.3274
6.7474
t3m2
.11333
1.38283
.935
-2.7407
2.9674
t3m3
1.18000
1.38283
.402
-1.6740
4.0340
t3m4
1.44333
1.38283
.307
-1.4107
4.2974
t1m1
-1.98000
1.38283
.165
-4.8340
.8740
t1m2
*
1.38283
.039
-5.8740
-.1660
t1m3
-1.75667
1.38283
.216
-4.6107
1.0974
t1m4
t2m1
-2.56333
-5.20333*
1.38283
1.38283
.076
.001
-5.4174
-8.0574
.2907
-2.3493
t2m3
-3.25667*
1.38283
.027
-6.1107
-.4026
t2m4
-1.73000
1.38283
.223
-4.5840
1.1240
t3m1
-1.31000
1.38283
.353
-4.1640
1.5440
t3m2
-5.09000
*
1.38283
.001
-7.9440
-2.2360
t3m3
-4.02333
*
1.38283
.008
-6.8774
-1.1693
t3m4
-3.76000*
1.38283
.012
-6.6140
-.9060
t1m1
1.27667
1.38283
.365
-1.5774
4.1307
t1m2
.23667
1.38283
.866
-2.6174
3.0907
t1m3
1.50000
1.38283
.289
-1.3540
4.3540
t1m4
.69333
1.38283
.621
-2.1607
3.5474
.9074
6.1107
t2m1
t2m2
t2m4
-3.02000
bio.unsoed.ac.id
-1.94667
1.38283
.172
*
1.38283
.027
-4.8007
.4026
t2m4
1.52667
1.38283
.281
-1.3274
4.3807
t3m1
1.94667
1.38283
.172
-.9074
4.8007
t3m2
-1.83333
1.38283
.197
-4.6874
1.0207
t3m3
-.76667
1.38283
.584
-3.6207
2.0874
t3m4
-.50333
1.38283
.719
-3.3574
2.3507
t1m1
-.25000
1.38283
.858
-3.1040
2.6040
3.25667
73
t3m1
t3m2
t3m3
t1m2
-1.29000
1.38283
.360
-4.1440
1.5640
t1m3
-.02667
1.38283
.985
-2.8807
2.8274
t1m4
-.83333
1.38283
.552
-3.6874
2.0207
t2m1
t2m2
*
-3.47333
1.73000
1.38283
1.38283
.019
.223
-6.3274
-1.1240
-.6193
4.5840
t2m3
-1.52667
1.38283
.281
-4.3807
1.3274
t3m1
.42000
1.38283
.764
-2.4340
3.2740
t3m2
t3m3
*
-3.36000
-2.29333
1.38283
1.38283
.023
.110
-6.2140
-5.1474
-.5060
.5607
t3m4
-2.03000
1.38283
.155
-4.8840
.8240
t1m1
t1m2
-.67000
-1.71000
1.38283
1.38283
.632
.228
-3.5240
-4.5640
2.1840
1.1440
t1m3
-.44667
1.38283
.749
-3.3007
2.4074
t1m4
-1.25333
1.38283
.374
-4.1074
1.6007
t2m1
t2m2
*
-3.89333
1.31000
1.38283
1.38283
.010
.353
-6.7474
-1.5440
-1.0393
4.1640
t2m3
-1.94667
1.38283
.172
-4.8007
.9074
t2m4
-.42000
1.38283
.764
-3.2740
2.4340
t3m2
*
1.38283
.012
-6.6340
-.9260
t3m3
-2.71333
1.38283
.061
-5.5674
.1407
t3m4
-2.45000
1.38283
.089
-5.3040
.4040
t1m1
*
3.11000
1.38283
.034
.2560
5.9640
t1m2
t1m3
2.07000
3.33333*
1.38283
1.38283
.147
.024
-.7840
.4793
4.9240
6.1874
t1m4
2.52667
1.38283
.080
-.3274
5.3807
t2m1
-.11333
1.38283
.935
-2.9674
2.7407
t2m2
*
1.38283
.001
2.2360
7.9440
t2m3
1.83333
1.38283
.197
-1.0207
4.6874
t2m4
*
3.36000
1.38283
.023
.5060
6.2140
t3m1
3.78000*
1.38283
.012
.9260
6.6340
t3m3
1.06667
1.38283
.448
-1.7874
3.9207
t3m4
1.33000
1.38283
.346
-1.5240
4.1840
t1m1
2.04333
1.38283
.153
-.8107
4.8974
t1m2
1.00333
1.38283
.475
-1.8507
3.8574
5.1207
4.3140
t1m3
t1m4
-3.78000
5.09000
bio.unsoed.ac.id
2.26667
1.38283
.114
1.46000
1.38283
.302
-.5874
-1.3940
t2m1
-1.18000
1.38283
.402
-4.0340
1.6740
t2m2
*
1.38283
.008
1.1693
6.8774
t2m3
.76667
1.38283
.584
-2.0874
3.6207
t2m4
2.29333
1.38283
.110
-.5607
5.1474
t3m1
2.71333
1.38283
.061
-.1407
5.5674
t3m2
-1.06667
1.38283
.448
-3.9207
1.7874
4.02333
73
t3m4
.26333
1.38283
.851
-2.5907
3.1174
t1m1
1.78000
1.38283
.210
-1.0740
4.6340
t1m2
.74000
1.38283
.597
-2.1140
3.5940
t1m3
t1m4
2.00333
1.19667
1.38283
1.38283
.160
.395
-.8507
-1.6574
4.8574
4.0507
t2m1
-1.44333
1.38283
.307
-4.2974
1.4107
t2m2
*
1.38283
.012
.9060
6.6140
t2m3
t2m4
.50333
2.03000
1.38283
1.38283
.719
.155
-2.3507
-.8240
3.3574
4.8840
t3m1
2.45000
1.38283
.089
-.4040
5.3040
t3m2
-1.33000
1.38283
t3m3
-.26333
1.38283
*. The mean difference is significant at the 0.05 level.
.346
.851
-4.1840
-3.1174
1.5240
2.5907
t3m4
3.76000
bio.unsoed.ac.id
73
No.
1.
Nama Alat
Akuarium
Merek/Tipe
Kegunaan
Tempat pemeliharaan
ikan uji
2.
Aerator
Amara
Sumber oksigen
3.
Timbangan
analitik
Ohaus
4.
Gelas ukur
Iwaki TE32 PYREX
Menimbang bubuk
metiltestosteron dan
aliuzarin red
Mengukur volume
larutan
5.
Beaker glass
Tempat menampung
larutan
6.
Milimeter blok
Iwaki,
PYREX
50ml
-
7.
Pipet tetes
-
Untuk memindahkan
larutan
8.
Timbangan
analitik
CHQ
Menimbang berat
ikan uji
9.
Piring kecil
-
10.
Mikroskop
binokuler
Olympus
CH20
11.
Termostat
12.
Spuit injeksi
Sobo
Akuaking
Amara
OneMed
1ml
Tempat
mencampurkan milt
dan sel telur
Mengamati jaringan
histologis sirip ikan
uji
Setting temperatur
13.
Saringan
Mengukur panjang
tubuh ikan uji
Untuk injeksi
ovaprim ke tubuh
induk ikan uji
Memisahkan sel telur
dengan seminal
plasma sperma pada
saat pemijahan
bio.unsoed.ac.id
-
44
Tempat
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
No.
Nama Alat
Merek/Tipe
Kegunaan
Tempat
14.
Kamera
Nikon
Mendokumentasikan
jaringan histologis
sirip
Lab. Struktur
Perkembangan
Hewan
15.
Eye piece
micrometer
Olympus
Mengukur panjang dan
luas sirip ikan uji
Lab. Struktur
Perkembangan
Hewan
16.
Botol sampel
-
Meletakan sampel
sirip ikan uji
17.
Kertas label
-
Mencatat informasi
sampel
18.
Cavity slide
-
Meletakkan ikan yang
akan diamati dibawah
mikrooskop
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
Lab. Struktur
Perkembangan
Hewan
19.
Magnetic stirer
Menghomogenkan
larutan alizarin red,
penjernih A, B, dan C
bio.unsoed.ac.id
44
Lab. Fisiologi
Tumbuhan
No.
1.
Nama Bahan
Ovaprim
Spesifikasi
Syndel 10ml
2.
Alkohol 70%
C2H5OH Mol: 46,07 g/mol
3.
17αMetiltestosteron
Mol:302,451 mg/L
4.
Pelet
Protein 41%, Lemak 6%, Serat 2,5%,
Abu 16%, Kelembaban 11%
5.
KOH 1%
Menjernihkan
ikan uji
6.
Asam asetat glasial
7.
Alizarin Red
8.
Gliserin PA
9.
Alkohol 1%
10.
KOH 2%
11.
KOH 4%
12.
Gliserin
13.
14.
15.
16.
Alkohol 96%
Akuades
Larva ikan nilem
Induk ikan nilem
Pembuatan larutan
alizarin red
Mewarnai sirip ikan
uji
Pembuatan pewarna
alizarin red
Pembuatan pewarna
alizarin red
Pelarut alizarin red
preparat histologis
Pembuatan larutan
penjernih A dan B
Pembuatan larutan
penjernih A, B dan C
Fiksasi ikan uji
Pelarut
Ikan uji
Penyedia ikan uji
C14H8O4. pH 5,8-7,2
Usia 1 hari penetasan
Matang gonad
bio.unsoed.ac.id
44
Kegunaan
Induksi induk ikan
uji
Pelarut
metiltestosteron
Larutan yang
digunakan sebagai
medium
pemeliharaan ikan uji
Pakan ikan uji
otot
Lampiran 2. Diagram alir penelitian
Pemijahan dan Penyediaan Ikan Uji
Persiapan Bak Pemeliharaan
Setting temperatur
Pembuatan Medium Pemeliharaan Ikan uji
Pemeliharaan Ikan Uji
Pengambilan data
perkembangan sirip secara
mikroskopis
Evaluasi Perkembangan sirip
secara makroskopis
Pewarnaan sirip
menggunakan metode
alizarin red
Analisis Data
Evaluasi hasil pewarnaan
bio.unsoed.ac.id
44
Lampiran 3. Hasil analisis ANOVA panjang dan luas sirip caudal ikan Nilem
(O. hasselti) yang dipelihara dalam medium dengan temperatur
dan kadar metiltestosteron berbeda.
1. Panjang Sirip Caudal
Tests of Between-Subjects Effects
Dependent Variable:PANJANG SIRIP CAUDAL
Type III Sum
of Squares
Source
Intercept
Hypothesis
Error
Suhu
Hypothesis
Error
Suhu * Ulangan
Hypothesis
Error
Hormon
Hypothesis
Error
Suhu * Hormon
Hypothesis
Error
a. MS(suhu * ulangan)
b. MS(Error)
df
Mean Square
6.706E7
1345985.642
260677.867
1345985.642
1345985.642
3202939.998
846965.477
3202939.998
3178602.112
3202939.998
F
1
6.706E7
6
2
6
6
18
3
18
6
18
a
Sig.
298.947
.000
.581
.588
1.261
.323
1.587
.227
2.977
.034
224330.940
130338.934
224330.940a
224330.940
177941.111b
282321.826
177941.111b
529767.019
177941.111b
Descriptives
Panjang Sirip Caudal
Interaksi
perlakuan
N
Mean
Std.
Deviation
95% Confidence Interval
for Mean
Std. Error
Minimum Maximum
Lower
Bound
t1m1
3 1.1723E3 431.43603 2.49090E2
t1m2
3 1.6596E3
t1m3
3 1.0961E3 207.11204 1.19576E2
t1m4
Upper Bound
100.5735
2244.0665
718.36
1577.00
51.85859 1436.4838
1882.7428
1574.30
1753.35
581.6552
1610.6448
868.90
1274.30
3 1.2199E3 504.06203 2.91020E2
-32.2262
2472.0928
914.44
1801.73
t2m1
3 2.1562E3 554.19036 3.19962E2
779.5448
3532.9152
1751.19
2787.80
t2m2
3 9.1890E2 456.11008 2.63335E2
-214.1402
2051.9402
439.85
1347.95
t2m3
3 1.7542E3 901.55101 5.20511E2
3993.8269
768.90
2537.80
t2m4
3 1.1037E3 119.19750
68.81871
807.5703
1399.7763
968.90
1195.25
t3m1
3 1.5567E3 313.22895 1.80843E2
778.6295
2334.8372
1195.25
1747.95
t3m2
3 1.3143E3 178.32206 1.02954E2
871.3601
1757.3132
1121.60
1473.46
t3m3
3 1.0117E3 220.48833 1.27299E2
463.9300
1559.3767
786.36
1227.00
t3m4
3 1.4146E3 512.43734 2.95856E2
141.6517
2687.5816
1068.90
2003.35
83.73793 1194.8705
1534.8645
439.85
2787.80
Total
89.82171
bio.unsoed.ac.id
-485.3269
36 1.3649E3 502.42757
73
Multiple Comparisons
Panjang Sirip Caudal
LSD
(I)
(J)
Interak Interak
Mean
si
si
Difference (I-J) Std. Error
t1m1
t1m2
t1m3
Sig.
Lower Bound Upper Bound
t1m2
-487.29333 3.55470E2
.183
-1220.9477
246.3611
t1m3
76.17000 3.55470E2
.832
-657.4844
809.8244
t1m4
-47.61333 3.55470E2
.895
-781.2677
686.0411
*
t2m1
-983.91000 3.55470E2
.011
-1717.5644
-250.2556
t2m2
253.42000 3.55470E2
.483
-480.2344
987.0744
t2m3
-581.93000 3.55470E2
.115
-1315.5844
151.7244
t2m4
68.64667 3.55470E2
.848
-665.0077
802.3011
t3m1
-384.41333 3.55470E2
.290
-1118.0677
349.2411
t3m2
-142.01667 3.55470E2
.693
-875.6711
591.6377
t3m3
160.66667 3.55470E2
.655
-572.9877
894.3211
t3m4
t1m1
-242.29667 3.55470E2
487.29333 3.55470E2
.502
.183
-975.9511
-246.3611
491.3577
1220.9477
t1m3
563.46333 3.55470E2
.126
-170.1911
1297.1177
t1m4
t2m1
439.68000 3.55470E2
-496.61667 3.55470E2
.228
.175
-293.9744
-1230.2711
1173.3344
237.0377
t2m2
740.71333* 3.55470E2
.048
7.0589
1474.3677
t2m3
-94.63667 3.55470E2
.792
-828.2911
639.0177
t2m4
t3m1
555.94000 3.55470E2
102.88000 3.55470E2
.131
.775
-177.7144
-630.7744
1289.5944
836.5344
t3m2
345.27667 3.55470E2
.341
-388.3777
1078.9311
t3m3
647.96000 3.55470E2
.081
-85.6944
1381.6144
t3m4
t1m1
244.99667 3.55470E2
-76.17000 3.55470E2
.497
.832
-488.6577
-809.8244
978.6511
657.4844
t1m2
-563.46333 3.55470E2
.126
-1297.1177
170.1911
t1m4
-123.78333 3.55470E2
.731
-857.4377
609.8711
*
t2m1
-1060.08000 3.55470E2
.006
-1793.7344
-326.4256
t2m2
177.25000 3.55470E2
.623
-556.4044
910.9044
t2m3
-658.10000 3.55470E2
.076
-1391.7544
75.5544
t2m4
t1m4
95% Confidence Interval
bio.unsoed.ac.id
-7.52333 3.55470E2
.983
-741.1777
726.1311
t3m1
-460.58333 3.55470E2
.207
-1194.2377
273.0711
t3m2
-218.18667 3.55470E2
.545
-951.8411
515.4677
t3m3
84.49667 3.55470E2
.814
-649.1577
818.1511
t3m4
-318.46667 3.55470E2
.379
-1052.1211
415.1877
t1m1
t1m2
47.61333 3.55470E2
-439.68000 3.55470E2
.895
.228
-686.0411
-1173.3344
781.2677
293.9744
t1m3
123.78333 3.55470E2
.731
-609.8711
857.4377
73
t2m1
t2m2
t2m3
t2m4
t2m1
-936.29667* 3.55470E2
.015
-1669.9511
-202.6423
t2m2
301.03333 3.55470E2
.405
-432.6211
1034.6877
t2m3
-534.31667 3.55470E2
.146
-1267.9711
199.3377
t2m4
t3m1
116.26000 3.55470E2
-336.80000 3.55470E2
.746
.353
-617.3944
-1070.4544
849.9144
396.8544
t3m2
-94.40333 3.55470E2
.793
-828.0577
639.2511
t3m3
208.28000 3.55470E2
.563
-525.3744
941.9344
t3m4
t1m1
-194.68333 3.55470E2
983.91000* 3.55470E2
.589
.011
-928.3377
250.2556
538.9711
1717.5644
t1m2
496.61667 3.55470E2
.175
-237.0377
1230.2711
t1m3
t1m4
*
1060.08000 3.55470E2
936.29667* 3.55470E2
.006
.015
326.4256
202.6423
1793.7344
1669.9511
t2m2
1237.33000* 3.55470E2
.002
503.6756
1970.9844
t2m3
401.98000 3.55470E2
.269
-331.6744
1135.6344
*
t2m4
t3m1
1052.55667 3.55470E2
599.49667 3.55470E2
.007
.105
318.9023
-134.1577
1786.2111
1333.1511
t3m2
841.89333* 3.55470E2
.026
108.2389
1575.5477
t3m3
1144.57667* 3.55470E2
.004
410.9223
1878.2311
t3m4
*
741.61333 3.55470E2
.048
7.9589
1475.2677
t1m1
-253.42000 3.55470E2
.483
-987.0744
480.2344
*
t1m2
-740.71333 3.55470E2
.048
-1474.3677
-7.0589
t1m3
-177.25000 3.55470E2
.623
-910.9044
556.4044
t1m4
t2m1
-301.03333 3.55470E2
-1237.33000* 3.55470E2
.405
.002
-1034.6877
-1970.9844
432.6211
-503.6756
t2m3
-835.35000* 3.55470E2
.027
-1569.0044
-101.6956
t2m4
-184.77333 3.55470E2
.608
-918.4277
548.8811
t3m1
-637.83333 3.55470E2
.085
-1371.4877
95.8211
t3m2
-395.43667 3.55470E2
.277
-1129.0911
338.2177
t3m3
-92.75333 3.55470E2
.796
-826.4077
640.9011
t3m4
-495.71667 3.55470E2
.176
-1229.3711
237.9377
t1m1
581.93000 3.55470E2
.115
-151.7244
1315.5844
t1m2
94.63667 3.55470E2
.792
-639.0177
828.2911
t1m3
658.10000 3.55470E2
.076
-75.5544
1391.7544
t1m4
534.31667 3.55470E2
.146
-199.3377
1267.9711
t2m1
t2m2
-401.98000 3.55470E2
835.35000* 3.55470E2
.027
101.6956
331.6744
1569.0044
t2m4
650.57667 3.55470E2
.080
-83.0777
1384.2311
t3m1
197.51667 3.55470E2
.584
-536.1377
931.1711
t3m2
439.91333 3.55470E2
.228
-293.7411
1173.5677
bio.unsoed.ac.id
.269
-1135.6344
*
t3m3
742.59667 3.55470E2
.047
8.9423
1476.2511
t3m4
339.63333 3.55470E2
.349
-394.0211
1073.2877
t1m1
-68.64667 3.55470E2
.848
-802.3011
665.0077
73
t3m1
t3m2
t3m3
t1m2
-555.94000 3.55470E2
.131
-1289.5944
177.7144
t1m3
7.52333 3.55470E2
.983
-726.1311
741.1777
t1m4
-116.26000 3.55470E2
.746
-849.9144
617.3944
*
t2m1
t2m2
-1052.55667 3.55470E2
184.77333 3.55470E2
.007
.608
-1786.2111
-548.8811
-318.9023
918.4277
t2m3
-650.57667 3.55470E2
.080
-1384.2311
83.0777
t3m1
-453.06000 3.55470E2
.215
-1186.7144
280.5944
t3m2
t3m3
-210.66333 3.55470E2
92.02000 3.55470E2
.559
.798
-944.3177
-641.6344
522.9911
825.6744
t3m4
-310.94333 3.55470E2
.390
-1044.5977
422.7111
t1m1
t1m2
384.41333 3.55470E2
-102.88000 3.55470E2
.290
.775
-349.2411
-836.5344
1118.0677
630.7744
t1m3
460.58333 3.55470E2
.207
-273.0711
1194.2377
t1m4
336.80000 3.55470E2
.353
-396.8544
1070.4544
t2m1
t2m2
-599.49667 3.55470E2
637.83333 3.55470E2
.105
.085
-1333.1511
-95.8211
134.1577
1371.4877
t2m3
-197.51667 3.55470E2
.584
-931.1711
536.1377
t2m4
453.06000 3.55470E2
.215
-280.5944
1186.7144
t3m2
242.39667 3.55470E2
.502
-491.2577
976.0511
t3m3
545.08000 3.55470E2
.138
-188.5744
1278.7344
t3m4
142.11667 3.55470E2
.693
-591.5377
875.7711
t1m1
142.01667 3.55470E2
.693
-591.6377
875.6711
t1m2
t1m3
-345.27667 3.55470E2
218.18667 3.55470E2
.341
.545
-1078.9311
-515.4677
388.3777
951.8411
t1m4
94.40333 3.55470E2
.793
-639.2511
828.0577
*
t2m1
-841.89333 3.55470E2
.026
-1575.5477
-108.2389
t2m2
395.43667 3.55470E2
.277
-338.2177
1129.0911
t2m3
-439.91333 3.55470E2
.228
-1173.5677
293.7411
t2m4
210.66333 3.55470E2
.559
-522.9911
944.3177
t3m1
-242.39667 3.55470E2
.502
-976.0511
491.2577
t3m3
302.68333 3.55470E2
.403
-430.9711
1036.3377
t3m4
-100.28000 3.55470E2
.780
-833.9344
633.3744
t1m1
-160.66667 3.55470E2
.655
-894.3211
572.9877
t1m2
-647.96000 3.55470E2
.081
-1381.6144
85.6944
bio.unsoed.ac.id
-84.49667 3.55470E2
.814
-818.1511
t1m3
t1m4
-208.28000 3.55470E2
.563
-941.9344
649.1577
525.3744
t2m1
-1144.57667* 3.55470E2
.004
-1878.2311
-410.9223
t2m2
92.75333 3.55470E2
.796
-640.9011
826.4077
*
t2m3
-742.59667 3.55470E2
.047
-1476.2511
-8.9423
t2m4
-92.02000 3.55470E2
.798
-825.6744
641.6344
t3m1
-545.08000 3.55470E2
.138
-1278.7344
188.5744
t3m2
-302.68333 3.55470E2
.403
-1036.3377
430.9711
73
t3m4
-402.96333 3.55470E2
.268
-1136.6177
330.6911
t1m1
242.29667 3.55470E2
.502
-491.3577
975.9511
t1m2
-244.99667 3.55470E2
.497
-978.6511
488.6577
t1m3
t1m4
318.46667 3.55470E2
194.68333 3.55470E2
.379
.589
-415.1877
-538.9711
1052.1211
928.3377
t2m1
-741.61333* 3.55470E2
.048
-1475.2677
-7.9589
t2m2
495.71667 3.55470E2
.176
-237.9377
1229.3711
t2m3
t2m4
-339.63333 3.55470E2
310.94333 3.55470E2
.349
.390
-1073.2877
-422.7111
394.0211
1044.5977
t3m1
-142.11667 3.55470E2
.693
-875.7711
591.5377
t3m2
100.28000 3.55470E2
.780
t3m3
402.96333 3.55470E2
.268
*. The mean difference is significant at the 0.05 level.
-633.3744
-330.6911
833.9344
1136.6177
t3m4
2. Luas Sirip Caudal
Tests of Between-Subjects Effects
Dependent Variable:LUAS SIRIP CAUDAL
Type III Sum
of Squares
Source
Intercept
Suhu
Suhu * Ulangan
Hormon
Suhu * Hormon
Hypothesis
df
Mean Square
2.111E7
1
2.111E7
Error
1175414.280
6
195902.380a
Hypothesis
1220837.642
2
610418.821
a
Error
1175414.280
6
195902.380
Hypothesis
1175414.280
6
195902.380
Error
3336345.851
18
185352.547b
Hypothesis
2407338.413
3
802446.138
b
Error
3336345.851
18
Hypothesis
2797308.143
6
466218.024
Error
3336345.851
18
185352.547b
a. MS(Suhu * Ulangan)
b. MS(Error)
F
bio.unsoed.ac.id
73
Sig.
107.748
.000
3.116
.118
1.057
.423
4.329
.018
2.515
.060
185352.547
Multiple Comparisons
Descriptives
LUAS SIRIP
CAUDAL
Metiltestost
eron
KONTROL
1,6 nM
16 nM
32 nM
Total
Mean
Std.
Deviation
Std. Error
1.1736E3
6.0507E2
7.9179E2
4.9249E2
7.6573E2
595.87269
305.20786
775.91967
126.37564
559.01046
1.98624E2
1.01736E2
2.58640E2
42.12521
93.16841
N
9
9
9
9
36
95% Confidence Interval
for Mean
Lower
Bound
Upper
Bound
715.5361 1631.5927
370.4665 839.6735
195.3620 1388.2113
395.3446 589.6265
576.5847 954.8686
Minimum Maximum
374.99
1.75
124.45
222.34
1.75
2436.05
1081.06
2633.48
670.93
2633.48
LUAS SIRIP CAUDAL
LSD
(I)
(J)
METILTES METILTES
Mean
Std. Error
TOSTERO TOSTERO Difference (I-J)
N
N
95% Confidence Interval
Sig.
Lower Bound Upper Bound
KONTROL 1,6 nM
568.49444* 2.43383E2
.026
72.7388
1064.2501
16 nM
381.77778 2.43383E2
.127
-113.9779
877.5334
*
.009
185.3232
1176.8346
*
-568.49444 2.43383E2
.026
-1064.2501
-72.7388
16 nM
32 nM
-186.71667 2.43383E2
112.58444 2.43383E2
.449
.647
-682.4723
-383.1712
309.0390
608.3401
KONTROL
-381.77778 2.43383E2
.127
-877.5334
113.9779
186.71667 2.43383E2
.449
-309.0390
682.4723
299.30111 2.43383E2
-681.07889* 2.43383E2
.228
.009
-196.4546
-1176.8346
795.0568
-185.3232
-112.58444 2.43383E2
.647
-608.3401
383.1712
16 nM
-299.30111 2.43383E2
*. The mean difference is significant at the 0.05 level.
.228
-795.0568
196.4546
32 nM
1,6 nM
16 nM
681.07889 2.43383E2
KONTROL
1,6 nM
32 nM
32 nM
KONTROL
1,6 nM
bio.unsoed.ac.id
73
Lampiran 4. Hasil analisis ANOVA panjang dan luas sirip dorsal ikan Nilem
(O. hasselti) yang dipelihara dalam medium dengan temperatur
dan kadar metiltestosteron berbeda.
1. Panjang Sirip Dorsal
Tests of Between-Subjects Effects
Dependent Variable:PANJANG SIRIP DORSAL
Type III Sum
of Squares
Source
Intercept
df
Mean Square
Hypothesis
1.450E7
1
1.450E7
Error
Suhu
Hypothesis
Error
Suhu * Ulangan
Hypothesis
Error
Hormon
Hypothesis
Error
Suhu * Hormon
Hypothesis
Error
a. MS(Suhu * Ulangan)
b. MS(Error)
2590796.684
1043037.880
2590796.684
2590796.684
3245759.622
910321.145
3245759.622
148539.152
3245759.622
6
2
6
6
18
3
18
6
18
431799.447a
521518.940
431799.447a
431799.447
180319.979b
303440.382
180319.979b
24756.525
180319.979b
F
Sig.
33.571
.001
1.208
.362
2.395
.071
1.683
.206
.137
.989
2. Luas Sirip Dorsal
Tests of Between-Subjects Effects
Dependent Variable:LUAS SIRIP DORSAL
Type III Sum
of Squares
Source
Intercept
df
Mean Square
Hypothesis
1647188.287
1
1647188.287
Error
Suhu
Hypothesis
Error
Suhu * Ulangan
Hypothesis
Error
Hormon
Hypothesis
Error
Suhu * Hormon
Hypothesis
Error
a. MS(Suhu * Ulangan)
b. MS(Error)
677313.890
407069.857
677313.890
677313.890
355325.747
344106.914
355325.747
651482.341
355325.747
6
2
6
6
18
3
18
6
18
112885.648a
203534.929
112885.648a
112885.648
19740.319b
114702.305
19740.319b
108580.390
19740.319b
bio.unsoed.ac.id
73
F
Sig.
14.592
.009
1.803
.244
5.719
.002
5.811
.006
5.500
.002
Descriptives
LUAS SIRIP DORSAL
95% Confidence Interval
for Mean
Interaksi
t1m1
t1m2
t1m3
t1m4
t2m1
t2m2
t2m3
t2m4
t3m1
t3m2
t3m3
t3m4
Total
N
Mean
Std.
Deviation
Std. Error
Lower
Bound
3
3
3
3
3
3
3
3
3
3
3
3
36
1.4112E2
3.3387E2
47.4067
1.1452E2
7.1115E2
1.0631E2
5.0637E2
1.2644E2
2.3858E2
1.2216E2
87.0733
31.8633
2.1390E2
219.57102
158.44780
51.59707
177.67154
407.17165
151.33297
441.89402
111.57109
72.51268
76.66350
21.53881
27.66039
263.78014
1.26769E2
91.47988
29.78958
1.02579E2
2.35081E2
87.37213
2.55128E2
64.41560
41.86522
44.26169
12.43544
15.96973
43.96336
-404.3213
-59.7361
-80.7676
-326.8406
-300.3204
-269.6253
-591.3589
-150.7213
58.4452
-68.2794
33.5680
-36.8489
124.6544
Upper Bound Minimum Maximum
686.5680
727.4761
175.5809
555.8806
1722.6204
482.2386
1604.0923
403.5946
418.7082
312.6060
140.5787
100.5756
303.1551
4.32
180.67
3.24
9.05
281.21
15.68
76.42
3.24
154.86
76.42
66.42
14.86
3.24
Multiple Comparisons
LUAS SIRIP DORSAL
LSD
(I)
(J)
Mean
INTER INTER Difference
AKSI AKSI
(I-J)
t1m1
95% Confidence Interval
Std. Error
Sig.
Lower Bound
Upper Bound
t1m2
-192.74667 1.69365E2
.266
-542.2984
156.8051
t1m3
93.71667 1.69365E2
.585
-255.8351
443.2684
t1m4
26.60333 1.69365E2
.876
-322.9484
376.1551
t2m1
-570.02667* 1.69365E2
.003
-919.5784
-220.4749
t2m2
34.81667 1.69365E2
.839
-314.7351
bio.unsoed.ac.id
384.3684
t2m3
-365.24333* 1.69365E2
.041
-714.7951
-15.6916
t2m4
14.68667 1.69365E2
.932
-334.8651
364.2384
t3m1
-97.45333 1.69365E2
.570
-447.0051
252.0984
t3m2
18.96000 1.69365E2
.912
-330.5918
368.5118
t3m3
54.05000 1.69365E2
.752
-295.5018
403.6018
t3m4
109.26000 1.69365E2
.525
-240.2918
458.8118
73
394.39
497.09
104.12
319.65
1090.90
281.01
959.31
220.67
281.75
210.67
109.40
63.78
1090.90
t1m2
t1m3
t1m4
t2m1
t1m1
192.74667 1.69365E2
.266
-156.8051
542.2984
t1m3
286.46333 1.69365E2
.104
-63.0884
636.0151
t1m4
219.35000 1.69365E2
.208
-130.2018
568.9018
t2m1
-377.28000* 1.69365E2
.036
-726.8318
-27.7282
t2m2
227.56333 1.69365E2
.192
-121.9884
577.1151
t2m3
-172.49667 1.69365E2
.319
-522.0484
177.0551
t2m4
207.43333 1.69365E2
.233
-142.1184
556.9851
t3m1
95.29333 1.69365E2
.579
-254.2584
444.8451
t3m2
211.70667 1.69365E2
.223
-137.8451
561.2584
t3m3
246.79667 1.69365E2
.158
-102.7551
596.3484
t3m4
302.00667 1.69365E2
.087
-47.5451
651.5584
t1m1
-93.71667 1.69365E2
.585
-443.2684
255.8351
t1m2
-286.46333 1.69365E2
.104
-636.0151
63.0884
t1m4
-67.11333 1.69365E2
.695
-416.6651
282.4384
t2m1
-663.74333* 1.69365E2
.001
-1013.2951
-314.1916
t2m2
-58.90000 1.69365E2
.731
-408.4518
290.6518
t2m3
-458.96000* 1.69365E2
.012
-808.5118
-109.4082
t2m4
-79.03000 1.69365E2
.645
-428.5818
270.5218
t3m1
-191.17000 1.69365E2
.270
-540.7218
158.3818
t3m2
-74.75667 1.69365E2
.663
-424.3084
274.7951
t3m3
-39.66667 1.69365E2
.817
-389.2184
309.8851
t3m4
15.54333 1.69365E2
.928
-334.0084
365.0951
t1m1
-26.60333 1.69365E2
.876
-376.1551
322.9484
t1m2
-219.35000 1.69365E2
.208
-568.9018
130.2018
t1m3
67.11333 1.69365E2
.695
-282.4384
416.6651
t2m1
-596.63000* 1.69365E2
.002
-946.1818
-247.0782
t2m2
8.21333 1.69365E2
.962
-341.3384
357.7651
t2m3
-391.84667* 1.69365E2
.030
-741.3984
-42.2949
t2m4
-11.91667 1.69365E2
t3m1
-124.05667 1.69365E2
.471
-473.6084
225.4951
t3m2
-7.64333 1.69365E2
.964
-357.1951
341.9084
t3m3
27.44667 1.69365E2
.873
-322.1051
376.9984
t3m4
82.65667 1.69365E2
.630
-266.8951
432.2084
t1m1
570.02667* 1.69365E2
.003
220.4749
919.5784
t1m2
377.28000* 1.69365E2
.036
27.7282
726.8318
bio.unsoed.ac.id
.944
-361.4684
73
337.6351
t2m2
t2m3
t2m4
t1m3
663.74333* 1.69365E2
.001
314.1916
1013.2951
t1m4
596.63000* 1.69365E2
.002
247.0782
946.1818
t2m2
604.84333* 1.69365E2
.002
255.2916
954.3951
t2m3
204.78333 1.69365E2
.238
-144.7684
554.3351
t2m4
584.71333* 1.69365E2
.002
235.1616
934.2651
t3m1
472.57333* 1.69365E2
.010
123.0216
822.1251
t3m2
588.98667* 1.69365E2
.002
239.4349
938.5384
t3m3
624.07667* 1.69365E2
.001
274.5249
973.6284
t3m4
679.28667* 1.69365E2
.001
329.7349
1028.8384
t1m1
-34.81667 1.69365E2
.839
-384.3684
314.7351
t1m2
-227.56333 1.69365E2
.192
-577.1151
121.9884
t1m3
58.90000 1.69365E2
.731
-290.6518
408.4518
t1m4
-8.21333 1.69365E2
.962
-357.7651
341.3384
t2m1
-604.84333* 1.69365E2
.002
-954.3951
-255.2916
t2m3
-400.06000* 1.69365E2
.027
-749.6118
-50.5082
t2m4
-20.13000 1.69365E2
.906
-369.6818
329.4218
t3m1
-132.27000 1.69365E2
.442
-481.8218
217.2818
t3m2
-15.85667 1.69365E2
.926
-365.4084
333.6951
t3m3
19.23333 1.69365E2
.911
-330.3184
368.7851
t3m4
74.44333 1.69365E2
.664
-275.1084
423.9951
t1m1
365.24333* 1.69365E2
.041
15.6916
714.7951
t1m2
172.49667 1.69365E2
.319
-177.0551
522.0484
t1m3
458.96000* 1.69365E2
.012
109.4082
808.5118
t1m4
391.84667* 1.69365E2
.030
42.2949
741.3984
t2m1
-204.78333 1.69365E2
.238
-554.3351
144.7684
t2m2
400.06000* 1.69365E2
.027
50.5082
749.6118
t2m4
379.93000* 1.69365E2
.034
30.3782
729.4818
t3m1
267.79000 1.69365E2
.127
-81.7618
617.3418
t3m2
384.20333* 1.69365E2
t3m3
419.29333* 1.69365E2
.021
69.7416
768.8451
t3m4
474.50333* 1.69365E2
.010
124.9516
824.0551
t1m1
-14.68667 1.69365E2
.932
-364.2384
334.8651
t1m2
-207.43333 1.69365E2
.233
-556.9851
142.1184
t1m3
79.03000 1.69365E2
.645
-270.5218
428.5818
t1m4
11.91667 1.69365E2
.944
-337.6351
361.4684
bio.unsoed.ac.id
.033
34.6516
73
733.7551
t3m1
t3m2
t3m3
t2m1
-584.71333* 1.69365E2
.002
-934.2651
-235.1616
t2m2
20.13000 1.69365E2
.906
-329.4218
369.6818
t2m3
-379.93000* 1.69365E2
.034
-729.4818
-30.3782
t3m1
-112.14000 1.69365E2
.514
-461.6918
237.4118
t3m2
4.27333 1.69365E2
.980
-345.2784
353.8251
t3m3
39.36333 1.69365E2
.818
-310.1884
388.9151
t3m4
94.57333 1.69365E2
.582
-254.9784
444.1251
t1m1
97.45333 1.69365E2
.570
-252.0984
447.0051
t1m2
-95.29333 1.69365E2
.579
-444.8451
254.2584
t1m3
191.17000 1.69365E2
.270
-158.3818
540.7218
t1m4
124.05667 1.69365E2
.471
-225.4951
473.6084
t2m1
-472.57333* 1.69365E2
.010
-822.1251
-123.0216
t2m2
132.27000 1.69365E2
.442
-217.2818
481.8218
t2m3
-267.79000 1.69365E2
.127
-617.3418
81.7618
t2m4
112.14000 1.69365E2
.514
-237.4118
461.6918
t3m2
116.41333 1.69365E2
.498
-233.1384
465.9651
t3m3
151.50333 1.69365E2
.380
-198.0484
501.0551
t3m4
206.71333 1.69365E2
.234
-142.8384
556.2651
t1m1
-18.96000 1.69365E2
.912
-368.5118
330.5918
t1m2
-211.70667 1.69365E2
.223
-561.2584
137.8451
t1m3
74.75667 1.69365E2
.663
-274.7951
424.3084
t1m4
7.64333 1.69365E2
.964
-341.9084
357.1951
t2m1
-588.98667* 1.69365E2
.002
-938.5384
-239.4349
t2m2
15.85667 1.69365E2
.926
-333.6951
365.4084
t2m3
-384.20333* 1.69365E2
.033
-733.7551
-34.6516
t2m4
-4.27333 1.69365E2
.980
-353.8251
345.2784
t3m1
-116.41333 1.69365E2
.498
-465.9651
233.1384
t3m3
35.09000 1.69365E2
.838
-314.4618
384.6418
t3m4
90.30000 1.69365E2
t1m1
-54.05000 1.69365E2
.752
-403.6018
295.5018
t1m2
-246.79667 1.69365E2
.158
-596.3484
102.7551
t1m3
39.66667 1.69365E2
.817
-309.8851
389.2184
t1m4
-27.44667 1.69365E2
.873
-376.9984
322.1051
t2m1
-624.07667* 1.69365E2
.001
-973.6284
-274.5249
t2m2
-19.23333 1.69365E2
.911
-368.7851
330.3184
bio.unsoed.ac.id
.599
-259.2518
73
439.8518
t3m4
t2m3
-419.29333* 1.69365E2
.021
-768.8451
-69.7416
t2m4
-39.36333 1.69365E2
.818
-388.9151
310.1884
t3m1
-151.50333 1.69365E2
.380
-501.0551
198.0484
t3m2
-35.09000 1.69365E2
.838
-384.6418
314.4618
t3m4
55.21000 1.69365E2
.747
-294.3418
404.7618
t1m1
-109.26000 1.69365E2
.525
-458.8118
240.2918
t1m2
-302.00667 1.69365E2
.087
-651.5584
47.5451
t1m3
-15.54333 1.69365E2
.928
-365.0951
334.0084
t1m4
-82.65667 1.69365E2
.630
-432.2084
266.8951
t2m1
-679.28667* 1.69365E2
.001
-1028.8384
-329.7349
t2m2
-74.44333 1.69365E2
.664
-423.9951
275.1084
t2m3
-474.50333* 1.69365E2
.010
-824.0551
-124.9516
t2m4
-94.57333 1.69365E2
.582
-444.1251
254.9784
t3m1
-206.71333 1.69365E2
.234
-556.2651
142.8384
t3m2
-90.30000 1.69365E2
.599
-439.8518
259.2518
t3m3
-55.21000 1.69365E2
.747
-404.7618
294.3418
*. The mean difference is significant at the 0.05 level.
bio.unsoed.ac.id
73
Lampiran 5. Hasil analisis ANOVA panjang dan luas sirip pectoral ikan Nilem
(O. hasselti) yang dipelihara dalam medium dengan temperatur
dan kadar metiltestosteron berbeda.
1. Panjang Sirip Pectoral
Tests of Between-Subjects Effects
Dependent Variable:PANJANG SIRIP
PECTORAL
Type III Sum
of Squares
Source
Intercept
Hypothesis
Error
Suhu
Hypothesis
Error
Suhu * Ulangan
Hypothesis
Error
Hormon
Hypothesis
Error
Suhu * Hormon
Hypothesis
Error
a. MS(Suhu * Ulangan)
b. MS(Error)
df
3.302E7
235660.396
160167.880
235660.396
235660.396
1205159.976
157692.190
1205159.976
264515.166
1205159.976
Mean Square
F
1
3.302E7
6
2
6
6
18
3
18
6
18
a
39276.733
80083.940
39276.733a
39276.733
66953.332b
52564.063
66953.332b
44085.861
66953.332b
Sig.
840.711
.000
2.039
.211
.587
.737
.785
.518
.658
.684
2. Luas Sirip Pectoral
Tests of Between-Subjects Effects
Dependent Variable:LUAS SIRIP PECTORAL
Type III Sum
of Squares
Source
Intercept
Hypothesis
Error
Suhu
Hypothesis
Error
Suhu * Ulangan
Hypothesis
Error
Hormon
Hypothesis
Error
Suhu * Hormon
Hypothesis
Error
a. MS(Suhu * Ulangan)
b. MS(Error)
6334122.844
178644.242
23865.812
178644.242
178644.242
336287.995
137784.693
336287.995
181570.797
336287.995
df
Mean Square
1
6334122.844
6
2
6
6
18
3
18
6
18
a
bio.unsoed.ac.id
73
F
29774.040
11932.906
29774.040a
29774.040
18682.666b
45928.231
18682.666b
30261.800
18682.666b
Sig.
212.740
.000
.401
.686
1.594
.206
2.458
.096
1.620
.199
Lampiran 6. Hasil analisis ANOVA panjang dan luas sirip anal ikan Nilem
(O. hasselti) yang dipelihara dalam medium dengan temperatur
dan kadar metiltestosteron berbeda.
1. Panjang Sirip Anal
Tests of Between-Subjects Effects
Dependent Variable:PANJANG SIRIP ANAL
Type III Sum
of Squares
Source
Intercept
Hypothesis
Error
Suhu
Hypothesis
Error
Suhu * Ulangan
Hypothesis
Error
Hormon
Hypothesis
Error
Suhu * Hormon
Hypothesis
Error
a. MS(Suhu * Ulangan)
b. MS(Error)
df
Mean Square
1.283E7
522773.348
208797.072
522773.348
522773.348
1374520.002
1099169.873
1374520.002
1104762.639
1374520.002
F
1
1.283E7
6
2
6
6
18
3
18
6
18
a
Sig.
147.247
.000
1.198
.365
1.141
.379
4.798
.013
2.411
.069
Minimum
Maximum
87128.891
104398.536
87128.891a
87128.891
76362.222b
366389.958
76362.222b
184127.107
76362.222b
Descriptives
PANJANG SIRIP
ANAL
Metil
testosteron
KONTROL
1,6 nM
16 nM
32 nM
Total
N
9
9
9
9
36
Mean
8.8416E2
5.0951E2
5.7475E2
4.1946E2
5.9697E2
Std.
Deviation
Std. Error
380.16387 1.26721E2
214.15475 71.38492
360.38300 1.20128E2
284.76991 94.92330
350.91810 58.48635
95% Confidence
Interval for Mean
Lower
Bound
591.9402 1176.3798
344.8950 674.1228
297.7329 851.7627
200.5698 638.3569
478.2364 715.7036
bio.unsoed.ac.id
73
Upper
Bound
150.00
161.75
168.10
68.10
68.10
1421.60
837.15
1421.60
866.20
1421.60
Multiple Comparisons
PANJANG SIRIP ANAL
LSD
(I)
(J)
METILTES METILTES
Mean
Std. Error
TOSTERO TOSTERO Difference (I-J)
N
N
95% Confidence Interval
Sig.
374.65111* 1.49324E2
KONTROL 1,6 nM
*
Lower Bound Upper Bound
.017
70.4885
678.8137
16 nM
309.41222 1.49324E2
.046
5.2496
613.5748
32 nM
464.69667* 1.49324E2
.004
160.5341
768.8592
-374.65111 1.49324E2
.017
-678.8137
-70.4885
16 nM
-65.23889 1.49324E2
.665
-369.4015
238.9237
32 nM
90.04556 1.49324E2
.551
-214.1170
394.2081
-309.41222 1.49324E2
.046
-613.5748
-5.2496
1,6 nM
65.23889 1.49324E2
.665
-238.9237
369.4015
32 nM
155.28444 1.49324E2
.306
-148.8781
459.4470
-464.69667 1.49324E2
-90.04556 1.49324E2
.004
.551
-768.8592
-394.2081
-160.5341
214.1170
16 nM
-155.28444 1.49324E2
*. The mean difference is significant at the 0.05 level.
.306
-459.4470
148.8781
1,6 nM
16 nM
32 nM
KONTROL
KONTROL
KONTROL
1,6 nM
*
*
*
2. Luas Sirip Anal
Tests of Between-Subjects Effects
Dependent Variable:LUAS SIRIP ANAL
Type III Sum
of Squares
Source
Intercept
Suhu
Suhu * Ulangan
Hormon
Suhu * Hormon
Hypothesis
Mean Square
F
1
277623.610
6
2
6
6
18
3
18
6
a
9601.297
16079.496
9601.297a
9601.297
2647.986b
16324.507
2647.986b
11295.988
47663.751
18
bio.unsoed.ac.id
2647.986b
Error
Hypothesis
Error
Hypothesis
Error
Hypothesis
Error
Hypothesis
Error
277623.610
df
57607.783
32158.991
57607.783
57607.783
47663.751
48973.522
47663.751
67775.931
a. MS(Suhu * Ulangan)
b. MS(Error)
73
Sig.
28.915
.002
1.675
.264
3.626
.016
6.165
.005
4.266
.008
Descriptives
Luas Sirip Anal
95% Confidence Interval
for Mean
Interaksi
N
t1m1
t1m2
t1m3
t1m4
t2m1
t2m2
t2m3
t2m4
t3m1
t3m2
t3m3
t3m4
Total
3
3
3
3
3
3
3
3
3
3
3
3
36
Mean
Std.
Deviation Std. Error Lower Bound Upper Bound Minimum Maximum
42.3467 28.27991 16.32741
1.2628E2 45.16820 26.07787
43.1533
3.18957 1.84150
38.9433 15.60585 9.01004
2.4781E2 104.98236 60.61160
49.9100 26.63805 15.37949
1.5797E2 142.16000 82.07611
63.5667 77.30838 44.63402
1.3891E2 50.00094 28.86805
38.1533 23.85924 13.77514
82.6333 87.14720 50.31446
24.1200 31.00330 17.89977
87.8167 85.21904 14.20317
-27.9045
14.0760
35.2300
.1762
-12.9807
-16.2626
-195.1750
-128.4780
14.7041
-21.1163
-133.8523
-52.8965
58.9827
112.5978
238.4840
51.0767
77.7104
508.6007
116.0826
511.1150
255.6113
263.1225
97.4230
299.1190
101.1365
116.6506
25.75
90.06
41.02
21.02
152.16
21.29
15.81
6.83
101.08
13.92
21.55
3.65
3.65
Multiple Comparisons
Luas Sirip Anal
LSD
(I)
(J)
INTER INTER
Mean
AKSI AKSI Difference (I-J) Std. Error
t1m1
Sig.
Lower Bound Upper Bound
t1m2
-83.93333
54.07596
.134
-195.5406
27.6740
t1m3
-.80667
54.07596
.988
-112.4140
110.8006
t1m4
3.40333
54.07596
.950
-108.2040
115.0106
t2m1
*
54.07596
.001
-317.0706
-93.8560
t2m2
-7.56333
54.07596
.890
-119.1706
104.0440
t2m3
*
54.07596
.043
-227.2306
-4.0160
t2m4
-21.22000
54.07596
.698
-132.8273
90.3873
t3m1
-96.56667
54.07596
.087
-208.1740
15.0406
t3m2
4.19333
54.07596
.939
-107.4140
115.8006
t3m3
-40.28667
54.07596
.464
-151.8940
71.3206
t3m4
t1m2
95% Confidence Interval
-205.46333
-115.62333
bio.unsoed.ac.id
18.22667 54.07596
.739
-93.3806
129.8340
t1m1
83.93333
54.07596
.134
-27.6740
195.5406
t1m3
83.12667
54.07596
.137
-28.4806
194.7340
t1m4
t2m1
87.33667
-121.53000*
54.07596
54.07596
.119
.034
-24.2706
-233.1373
198.9440
-9.9227
t2m2
76.37000
54.07596
.171
-35.2373
187.9773
t2m3
-31.69000
54.07596
.563
-143.2973
79.9173
t2m4
62.71333
54.07596
.258
-48.8940
174.3206
73
75.00
176.89
46.82
49.52
360.13
73.98
300.13
151.62
195.60
61.62
182.43
59.79
360.13
t1m3
t1m4
t2m1
t3m1
-12.63333
54.07596
.817
-124.2406
98.9740
t3m2
88.12667
54.07596
.116
-23.4806
199.7340
t3m3
43.64667
54.07596
.428
-67.9606
155.2540
t3m4
t1m1
102.16000
.80667
54.07596
54.07596
.071
.988
-9.4473
-110.8006
213.7673
112.4140
t1m2
-83.12667
54.07596
.137
-194.7340
28.4806
t1m4
4.21000
54.07596
.939
-107.3973
115.8173
t2m1
t2m2
*
-204.65667
-6.75667
54.07596
54.07596
.001
.902
-316.2640
-118.3640
-93.0494
104.8506
t2m3
-114.81667*
54.07596
.044
-226.4240
-3.2094
t2m4
t3m1
-20.41333
-95.76000
54.07596
54.07596
.709
.089
-132.0206
-207.3673
91.1940
15.8473
t3m2
5.00000
54.07596
.927
-106.6073
116.6073
t3m3
-39.48000
54.07596
.472
-151.0873
72.1273
t3m4
t1m1
19.03333
-3.40333
54.07596
54.07596
.728
.950
-92.5740
-115.0106
130.6406
108.2040
t1m2
-87.33667
54.07596
.119
-198.9440
24.2706
t1m3
-4.21000
54.07596
.939
-115.8173
107.3973
t2m1
*
54.07596
.001
-320.4740
-97.2594
t2m2
-10.96667
54.07596
.841
-122.5740
100.6406
t2m3
*
54.07596
.038
-230.6340
-7.4194
t2m4
-24.62333
54.07596
.653
-136.2306
86.9840
t3m1
t3m2
-99.97000
.79000
54.07596
54.07596
.077
.988
-211.5773
-110.8173
11.6373
112.3973
t3m3
-43.69000
54.07596
.427
-155.2973
67.9173
t3m4
14.82333
54.07596
.786
-96.7840
126.4306
t1m1
*
54.07596
.001
93.8560
317.0706
*
54.07596
.034
9.9227
233.1373
t1m3
*
204.65667
54.07596
.001
93.0494
316.2640
t1m4
208.86667*
54.07596
.001
97.2594
320.4740
t2m2
*
54.07596
.001
86.2927
309.5073
t2m3
89.84000
54.07596
.110
-21.7673
201.4473
t2m4
*
54.07596
.002
72.6360
295.8506
t3m1
108.89667
54.07596
.055
-2.7106
220.5040
t3m2
t3m3
*
209.65667
165.17667*
54.07596
.005
53.5694
321.2640
276.7840
t3m4
223.69000*
54.07596
.000
112.0827
335.2973
t1m1
7.56333
54.07596
.890
-104.0440
119.1706
t1m2
-76.37000
54.07596
.171
-187.9773
35.2373
t1m3
6.75667
54.07596
.902
-104.8506
118.3640
t1m4
10.96667
54.07596
.841
-100.6406
122.5740
t2m1
*
54.07596
.001
-309.5073
-86.2927
t1m2
t2m2
-208.86667
-119.02667
205.46333
121.53000
197.90000
184.24333
bio.unsoed.ac.id
54.07596
.001
98.0494
-197.90000
73
t2m3
t2m4
t3m1
t2m3
-108.06000
54.07596
.057
-219.6673
3.5473
t2m4
-13.65667
54.07596
.803
-125.2640
97.9506
t3m1
-89.00333
54.07596
.113
-200.6106
22.6040
t3m2
t3m3
11.75667
-32.72333
54.07596
54.07596
.830
.551
-99.8506
-144.3306
123.3640
78.8840
t3m4
25.79000
54.07596
.638
-85.8173
137.3973
t1m1
*
115.62333
54.07596
.043
4.0160
227.2306
t1m2
t1m3
31.69000
114.81667*
54.07596
54.07596
.563
.044
-79.9173
3.2094
143.2973
226.4240
t1m4
119.02667*
54.07596
.038
7.4194
230.6340
t2m1
t2m2
-89.84000
108.06000
54.07596
54.07596
.110
.057
-201.4473
-3.5473
21.7673
219.6673
t2m4
94.40333
54.07596
.094
-17.2040
206.0106
t3m1
19.05667
54.07596
.728
-92.5506
130.6640
t3m2
t3m3
*
119.81667
75.33667
54.07596
54.07596
.036
.176
8.2094
-36.2706
231.4240
186.9440
t3m4
133.85000*
54.07596
.021
22.2427
245.4573
t1m1
21.22000
54.07596
.698
-90.3873
132.8273
t1m2
-62.71333
54.07596
.258
-174.3206
48.8940
t1m3
20.41333
54.07596
.709
-91.1940
132.0206
t1m4
24.62333
54.07596
.653
-86.9840
136.2306
t2m1
*
54.07596
.002
-295.8506
-72.6360
t2m2
t2m3
13.65667
-94.40333
54.07596
54.07596
.803
.094
-97.9506
-206.0106
125.2640
17.2040
t3m1
-75.34667
54.07596
.176
-186.9540
36.2606
t3m2
25.41333
54.07596
.643
-86.1940
137.0206
t3m3
-19.06667
54.07596
.727
-130.6740
92.5406
t3m4
39.44667
54.07596
.473
-72.1606
151.0540
t1m1
96.56667
54.07596
.087
-15.0406
208.1740
t1m2
12.63333
54.07596
.817
-98.9740
124.2406
t1m3
95.76000
54.07596
.089
-15.8473
207.3673
t1m4
99.97000
54.07596
.077
-11.6373
211.5773
t2m1
-108.89667
54.07596
.055
-220.5040
2.7106
t2m2
89.00333
54.07596
.113
-22.6040
200.6106
t2m3
t2m4
t3m2
-184.24333
bio.unsoed.ac.id
-19.05667 54.07596
.728
-130.6640
75.34667
54.07596
.176
-36.2606
92.5506
186.9540
t3m2
100.76000
54.07596
.075
-10.8473
212.3673
t3m3
56.28000
54.07596
.308
-55.3273
167.8873
t3m4
*
54.07596
.044
3.1860
226.4006
t1m1
-4.19333
54.07596
.939
-115.8006
107.4140
t1m2
-88.12667
54.07596
.116
-199.7340
23.4806
t1m3
-5.00000
54.07596
.927
-116.6073
106.6073
114.79333
73
t1m4
-.79000
54.07596
.988
-112.3973
110.8173
t2m1
*
54.07596
.001
-321.2640
-98.0494
t2m2
-11.75667
54.07596
.830
-123.3640
99.8506
t2m3
t2m4
*
-119.81667
-25.41333
54.07596
54.07596
.036
.643
-231.4240
-137.0206
-8.2094
86.1940
t3m1
-100.76000
54.07596
.075
-212.3673
10.8473
t3m3
-44.48000
54.07596
.419
-156.0873
67.1273
t3m4
t1m1
14.03333
40.28667
54.07596
54.07596
.797
.464
-97.5740
-71.3206
125.6406
151.8940
t1m2
-43.64667
54.07596
.428
-155.2540
67.9606
t1m3
t1m4
39.48000
43.69000
54.07596
54.07596
.472
.427
-72.1273
-67.9173
151.0873
155.2973
t2m1
-165.17667*
54.07596
.005
-276.7840
-53.5694
t2m2
32.72333
54.07596
.551
-78.8840
144.3306
t2m3
t2m4
-75.33667
19.06667
54.07596
54.07596
.176
.727
-186.9440
-92.5406
36.2706
130.6740
t3m1
-56.28000
54.07596
.308
-167.8873
55.3273
t3m2
44.48000
54.07596
.419
-67.1273
156.0873
t3m4
58.51333
54.07596
.290
-53.0940
170.1206
t1m1
-18.22667
54.07596
.739
-129.8340
93.3806
t1m2
-102.16000
54.07596
.071
-213.7673
9.4473
t1m3
-19.03333
54.07596
.728
-130.6406
92.5740
t1m4
t2m1
-14.82333
-223.69000*
54.07596
54.07596
.786
.000
-126.4306
-335.2973
96.7840
-112.0827
t2m2
-25.79000
54.07596
.638
-137.3973
85.8173
t2m3
*
54.07596
.021
-245.4573
-22.2427
t2m4
-39.44667
54.07596
.473
-151.0540
72.1606
t3m1
*
54.07596
.044
-226.4006
-3.1860
-14.03333
54.07596
.797
-125.6406
97.5740
t3m3
-58.51333 54.07596
.290
*. The mean difference is significant at the 0.05 level.
-170.1206
53.0940
t3m3
t3m4
t3m2
-209.65667
-133.85000
-114.79333
bio.unsoed.ac.id
73
Lampiran 7. Hasil analisis ANOVA panjang dan luas sirip abdominal ikan
Nilem (O. hasselti) yang dipelihara dalam medium dengan
temperatur dan kadar metiltestosteron berbeda.
1. Panjang Sirip Abdominal
Tests of Between-Subjects Effects
Dependent Variable:PANJANG SIRIP ABDOMINAL
Type III Sum
of Squares
Source
Intercept
Hypothesis
df
3.035E7
Error
Suhu
Hypothesis
Error
Suhu * Ulangan
Hypothesis
Error
Hormon
Hypothesis
Error
Suhu * Hormon
Hypothesis
Error
a. MS(Suhu * Ulangan)
b. MS(Error)
1224357.107
3807916.632
1224357.107
1224357.107
3391247.814
547611.738
3391247.814
705800.436
3391247.814
Mean Square
F
1
3.035E7
6
2
6
6
18
3
18
6
18
a
204059.518
1903958.316
204059.518a
204059.518
188402.656b
182537.246
188402.656b
117633.406
188402.656b
Sig.
148.719
.000
9.330
.014
1.083
.409
.969
.429
.624
.709
Descriptives
PANJANG SIRIP ABDOMINAL
95% Confidence
Interval for Mean
Temperat
ur
RUANG
27 C
30 C
Total
N
Mean
12
12
12
36
7.3494E2
1.3751E3
6.4438E2
9.1814E2
Std.
Deviation
Std. Error
Lower
Bound
Upper
Bound
483.85867 1.39678E2 427.5067 1042.3650
500.37365 1.44445E2 1057.1869 1693.0314
221.48140 63.93617 503.6624 785.1076
525.81729 87.63621 740.2324 1096.0543
Minimum Maximum
303.24
657.02
277.97
277.97
2021.60
2274.30
985.53
2274.30
2. Luas Sirip Abdominal
Tests of Between-Subjects Effects
Dependent Variable:LUAS SIRIP ABDOMINAL
Source
Intercept
Suhu
Suhu * Ulangan
Hormon
Type III Sum
of Squares
df
Mean Square
bio.unsoed.ac.id
Hypothesis
210752.151
1
210752.151
Error
Hypothesis
Error
Hypothesis
Error
Hypothesis
Error
101504.462
50137.835
101504.462
101504.462
146046.438
29179.143
146046.438
73
6
2
6
6
18
3
18
16917.410a
25068.917
16917.410a
16917.410
8113.691b
9726.381
8113.691b
F
Sig.
12.458
.012
1.482
.300
2.085
.106
1.199
.339
Suhu * Hormon
Hypothesis
Error
a. MS(Suhu * Ulangan)
b. MS(Error)
93295.429
146046.438
6
18
bio.unsoed.ac.id
73
15549.238
8113.691b
1.916
.133
Lampiran 8. Hasil analisis ANOVA panjang tubuh ikan Nilem (O. hasselti)
yang dipelihara dalam medium dengan temperatur dan kadar
metiltestosteron berbeda.
Tests of Between-Subjects Effects
Dependent Variable:PANJANG TUBUH
Type III Sum of
Squares
Source
Intercept
Hypothesis
Error
Hypothesis
Error
Hypothesis
Error
Hypothesis
Error
Hypothesis
Error
Suhu
Suhu * ulangan
Hormon
Suhu * hormon
df
1854.738
21.396
10.089
21.396
21.396
47.444
.616
47.444
69.444
47.444
Mean Square
F
1
1854.738
6
2
6
6
18
3
18
6
18
a
3.566
5.044
3.566a
3.566
2.636b
.205
2.636b
11.574
2.636b
Sig.
520.108
.000
1.415
.314
1.353
.286
.078
.971
4.391
.007
a. MS(suhu * ulangan)
b. MS(Error)
Descriptives
Panjang Tubuh
Interaksi
Perlakuan
N
Mean
Std.
Deviation
Std.
Error
95% Confidence Interval
for Mean
Lower
Bound
Minimum Maximum
Upper Bound
t1m1
3
6.3500
1.05825
.61098
3.7212
8.9788
5.13
7.02
t1m2
3
7.3900
1.58767
.91664
3.4460
11.3340
6.02
9.13
t1m3
3
6.1267
1.01908
.58837
3.5951
8.6582
5.24
7.24
t1m4
3
6.9333
1.39543
.80565
3.4669
10.3998
5.45
8.22
t2m1
3
9.5733
2.05627 1.18719
4.4653
14.6814
7.56
11.67
t2m2
3
4.3700
.38314
2.7215
6.0185
3.67
4.99
t2m3
3
7.6267
3.14518 1.81587
-.1864
15.4397
4.22
10.42
t2m4
3
6.1000
2.05939 1.18899
.9842
11.2158
4.61
8.45
t3m1
3
5.6800
9.3905
4.42
7.33
t3m2
3
9.4600
1.19578
.69039
6.4895
12.4305
8.24
10.63
t3m3
3
8.3933
2.30622 1.33149
2.6644
14.1223
5.90
10.45
t3m4
3
8.1300
.11000
.06351
7.8567
8.4033
8.02
8.24
Total
36
7.1778
2.06321
.34387
6.4797
7.8759
3.67
11.67
.66363
bio.unsoed.ac.id
1.49369 .86238
1.9695
73
Multiple Comparisons
Panjang Tubuh
LSD
(I)
Interaksi
t1m1
t1m2
t1m3
(J)
Interaksi
95% Confidence Interval
Std. Error
Sig.
Lower Bound
Upper Bound
t1m2
-1.04000
1.38283
.459
-3.8940
1.8140
t1m3
.22333
1.38283
.873
-2.6307
3.0774
t1m4
-.58333
1.38283
.677
-3.4374
2.2707
t2m1
*
1.38283
.028
-6.0774
-.3693
t2m2
1.98000
1.38283
.165
-.8740
4.8340
t2m3
-1.27667
1.38283
.365
-4.1307
1.5774
t2m4
.25000
1.38283
.858
-2.6040
3.1040
t3m1
.67000
1.38283
.632
-2.1840
3.5240
t3m2
*
1.38283
.034
-5.9640
-.2560
t3m3
-2.04333
1.38283
.153
-4.8974
.8107
t3m4
t1m1
-1.78000
1.04000
1.38283
1.38283
.210
.459
-4.6340
-1.8140
1.0740
3.8940
t1m3
1.26333
1.38283
.370
-1.5907
4.1174
t1m4
t2m1
.45667
-2.18333
1.38283
1.38283
.744
.127
-2.3974
-5.0374
3.3107
.6707
t2m2
3.02000*
1.38283
.039
.1660
5.8740
t2m3
-.23667
1.38283
.866
-3.0907
2.6174
t2m4
t3m1
1.29000
1.71000
1.38283
1.38283
.360
.228
-1.5640
-1.1440
4.1440
4.5640
t3m2
-2.07000
1.38283
.147
-4.9240
.7840
t3m3
-1.00333
1.38283
.475
-3.8574
1.8507
t3m4
t1m1
-.74000
-.22333
1.38283
1.38283
.597
.873
-3.5940
-3.0774
2.1140
2.6307
t1m2
-1.26333
1.38283
.370
-4.1174
1.5907
t1m4
-.80667
1.38283
.565
-3.6607
2.0474
t2m1
*
1.38283
.020
-6.3007
-.5926
t2m2
1.75667
1.38283
.216
-1.0974
4.6107
t2m3
-1.50000
1.38283
.289
-4.3540
1.3540
-2.8274
2.8807
t2m4
t1m4
Mean
Difference (IJ)
-3.22333
-3.11000
-3.44667
bio.unsoed.ac.id
.02667
1.38283
.985
t3m1
.44667
1.38283
.749
-2.4074
3.3007
t3m2
*
1.38283
.024
-6.1874
-.4793
t3m3
-2.26667
1.38283
.114
-5.1207
.5874
t3m4
-2.00333
1.38283
.160
-4.8574
.8507
t1m1
t1m2
.58333
-.45667
1.38283
1.38283
.677
.744
-2.2707
-3.3107
3.4374
2.3974
t1m3
.80667
1.38283
.565
-2.0474
3.6607
-3.33333
73
t2m1
t2m2
t2m3
t2m1
-2.64000
1.38283
.068
-5.4940
.2140
t2m2
2.56333
1.38283
.076
-.2907
5.4174
t2m3
-.69333
1.38283
.621
-3.5474
2.1607
t2m4
t3m1
.83333
1.25333
1.38283
1.38283
.552
.374
-2.0207
-1.6007
3.6874
4.1074
t3m2
-2.52667
1.38283
.080
-5.3807
.3274
t3m3
-1.46000
1.38283
.302
-4.3140
1.3940
t3m4
t1m1
-1.19667
3.22333*
1.38283
1.38283
.395
.028
-4.0507
.3693
1.6574
6.0774
t1m2
2.18333
1.38283
.127
-.6707
5.0374
t1m3
t1m4
*
3.44667
2.64000
1.38283
1.38283
.020
.068
.5926
-.2140
6.3007
5.4940
t2m2
5.20333*
1.38283
.001
2.3493
8.0574
t2m3
1.94667
1.38283
.172
-.9074
4.8007
t2m4
t3m1
*
3.47333
3.89333*
1.38283
1.38283
.019
.010
.6193
1.0393
6.3274
6.7474
t3m2
.11333
1.38283
.935
-2.7407
2.9674
t3m3
1.18000
1.38283
.402
-1.6740
4.0340
t3m4
1.44333
1.38283
.307
-1.4107
4.2974
t1m1
-1.98000
1.38283
.165
-4.8340
.8740
t1m2
*
1.38283
.039
-5.8740
-.1660
t1m3
-1.75667
1.38283
.216
-4.6107
1.0974
t1m4
t2m1
-2.56333
-5.20333*
1.38283
1.38283
.076
.001
-5.4174
-8.0574
.2907
-2.3493
t2m3
-3.25667*
1.38283
.027
-6.1107
-.4026
t2m4
-1.73000
1.38283
.223
-4.5840
1.1240
t3m1
-1.31000
1.38283
.353
-4.1640
1.5440
t3m2
-5.09000
*
1.38283
.001
-7.9440
-2.2360
t3m3
-4.02333
*
1.38283
.008
-6.8774
-1.1693
t3m4
-3.76000*
1.38283
.012
-6.6140
-.9060
t1m1
1.27667
1.38283
.365
-1.5774
4.1307
t1m2
.23667
1.38283
.866
-2.6174
3.0907
t1m3
1.50000
1.38283
.289
-1.3540
4.3540
t1m4
.69333
1.38283
.621
-2.1607
3.5474
.9074
6.1107
t2m1
t2m2
t2m4
-3.02000
bio.unsoed.ac.id
-1.94667
1.38283
.172
*
1.38283
.027
-4.8007
.4026
t2m4
1.52667
1.38283
.281
-1.3274
4.3807
t3m1
1.94667
1.38283
.172
-.9074
4.8007
t3m2
-1.83333
1.38283
.197
-4.6874
1.0207
t3m3
-.76667
1.38283
.584
-3.6207
2.0874
t3m4
-.50333
1.38283
.719
-3.3574
2.3507
t1m1
-.25000
1.38283
.858
-3.1040
2.6040
3.25667
73
t3m1
t3m2
t3m3
t1m2
-1.29000
1.38283
.360
-4.1440
1.5640
t1m3
-.02667
1.38283
.985
-2.8807
2.8274
t1m4
-.83333
1.38283
.552
-3.6874
2.0207
t2m1
t2m2
*
-3.47333
1.73000
1.38283
1.38283
.019
.223
-6.3274
-1.1240
-.6193
4.5840
t2m3
-1.52667
1.38283
.281
-4.3807
1.3274
t3m1
.42000
1.38283
.764
-2.4340
3.2740
t3m2
t3m3
*
-3.36000
-2.29333
1.38283
1.38283
.023
.110
-6.2140
-5.1474
-.5060
.5607
t3m4
-2.03000
1.38283
.155
-4.8840
.8240
t1m1
t1m2
-.67000
-1.71000
1.38283
1.38283
.632
.228
-3.5240
-4.5640
2.1840
1.1440
t1m3
-.44667
1.38283
.749
-3.3007
2.4074
t1m4
-1.25333
1.38283
.374
-4.1074
1.6007
t2m1
t2m2
*
-3.89333
1.31000
1.38283
1.38283
.010
.353
-6.7474
-1.5440
-1.0393
4.1640
t2m3
-1.94667
1.38283
.172
-4.8007
.9074
t2m4
-.42000
1.38283
.764
-3.2740
2.4340
t3m2
*
1.38283
.012
-6.6340
-.9260
t3m3
-2.71333
1.38283
.061
-5.5674
.1407
t3m4
-2.45000
1.38283
.089
-5.3040
.4040
t1m1
*
3.11000
1.38283
.034
.2560
5.9640
t1m2
t1m3
2.07000
3.33333*
1.38283
1.38283
.147
.024
-.7840
.4793
4.9240
6.1874
t1m4
2.52667
1.38283
.080
-.3274
5.3807
t2m1
-.11333
1.38283
.935
-2.9674
2.7407
t2m2
*
1.38283
.001
2.2360
7.9440
t2m3
1.83333
1.38283
.197
-1.0207
4.6874
t2m4
*
3.36000
1.38283
.023
.5060
6.2140
t3m1
3.78000*
1.38283
.012
.9260
6.6340
t3m3
1.06667
1.38283
.448
-1.7874
3.9207
t3m4
1.33000
1.38283
.346
-1.5240
4.1840
t1m1
2.04333
1.38283
.153
-.8107
4.8974
t1m2
1.00333
1.38283
.475
-1.8507
3.8574
5.1207
4.3140
t1m3
t1m4
-3.78000
5.09000
bio.unsoed.ac.id
2.26667
1.38283
.114
1.46000
1.38283
.302
-.5874
-1.3940
t2m1
-1.18000
1.38283
.402
-4.0340
1.6740
t2m2
*
1.38283
.008
1.1693
6.8774
t2m3
.76667
1.38283
.584
-2.0874
3.6207
t2m4
2.29333
1.38283
.110
-.5607
5.1474
t3m1
2.71333
1.38283
.061
-.1407
5.5674
t3m2
-1.06667
1.38283
.448
-3.9207
1.7874
4.02333
73
t3m4
.26333
1.38283
.851
-2.5907
3.1174
t1m1
1.78000
1.38283
.210
-1.0740
4.6340
t1m2
.74000
1.38283
.597
-2.1140
3.5940
t1m3
t1m4
2.00333
1.19667
1.38283
1.38283
.160
.395
-.8507
-1.6574
4.8574
4.0507
t2m1
-1.44333
1.38283
.307
-4.2974
1.4107
t2m2
*
1.38283
.012
.9060
6.6140
t2m3
t2m4
.50333
2.03000
1.38283
1.38283
.719
.155
-2.3507
-.8240
3.3574
4.8840
t3m1
2.45000
1.38283
.089
-.4040
5.3040
t3m2
-1.33000
1.38283
t3m3
-.26333
1.38283
*. The mean difference is significant at the 0.05 level.
.346
.851
-4.1840
-3.1174
1.5240
2.5907
t3m4
3.76000
bio.unsoed.ac.id
73