PRODUKSt SENYAWA BIOpLASIK P(3HB) DARI MINYAK KELAPA SAWIT DALAM BIOREAKTOR KAPASITAS 100 LITER DAN PEMANFAATANNYA SEBAGAI KEMASAN RAMAH LINGKUNGAN.
PRODUKSI SENYAIIA BIOPLASTIK P(3HBI
ALAIII BIOREAKTOR
I{APASITAS lOO LITER DAN PE]\iANFAATANNYA SEEACAI
akma DFma3f ' d:i aihoii Ascrei
-
bilyolEa,EsUsMab
da{hizd t c4 Fs d@md4cph,
€sae (NMR) ie hqna prcFd*
lotis we d 175 .c ft
(6c).
rH
d irc
nrb'
.c @{&r
€
(/ri, d he P€HB) sdrd vs 700 @o !
r
s
FEry
dd(rJ nd ,!r3
n4iers
d
um*an sem6
sehu:jd
(Pase. rsss)
an neryerabkai ebh dan sau
I'di.e'ewdre) shnsa
ratbD dan
rd*
6,ha €ah dbrukan
nurek
wr[n
jlb
P(3NA) di
dtai shy!
yans bdeune s
an masiesln (Lee ar
seiy*, pb3rkhd,hbruE
al
1se9
(boprtrrhdbpo*aipedamaka oeh
poims PI3EB) ie6eb
,esared,m (tui oka e,,r,1e3e) D'r
dmlai
keadaan nskh@nyaiq
eb lereklbi lesoo
P(3HB)
y,ns
eb h
r4si Pae
{oduks
*ry ,
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ks (Futu
dafoo, rsesr
D
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nsrunqan (bodescdsbe polmea sebasa bahai
dprc
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|s
sery
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ndaan bl€n
nrlii!
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R&Blseama 14Fm
sobary
3
n
@
Prcduksi
13Fn
tupbstik PttHB) daan bbteaktat
dan se
ais
reagenlDramaan
vdl!
6Fm seEah
dar lee3a
r3!h $mpa 66lam smpe
roe dai Yoo reel)
dambi
P(3HB)
Fru 1ed4a
d
rebrmrya d amb r dai
Larubo
N
P13HB)
Fre bah
3e
aijlhya
P13NB)
F4 €i'd
d ddamrya
henseidap re6eb
yaklndandkdnsbi padasdu ram lDramafl
c€{edesi trhad'o sery:ft
2004)
P(3HB)
*iy,
dib,ndnsbn deruai P(3NB)
ircdan 1r
rusnetk ht (NMFJ yat
NMR
lnrk
hiimd {rukd kmb P(3HB) Ffs dhasibndenean
F4deendabnoeh ooiard (re36,
P(3HB)
(GPC) denea' pe arui
k
ororom dan
mstods
1r36b)
Fru dhtrkao dGnlbn den@n
u !k ah! ebur (derrt
$
be6r bemi mdeku (Mu). puabiomor bed moretur (Mi) seda hdeb
bn
oreh
B
ham
er,r nee)
Mqd
Prcfi
pd
reme
*,
nd iediseipada
sntu
P(3BB) yans
P(3NB)d deanboek
jam ke 43
d*lmu4rya
d
mbr
sllk dmenb4,kn r;bh Trd
Bopo (usAl sesh
bed'nFmq
!F
d
beaenbs
tunilkan
Fe
Ganb{
1
iq deiq: i safqd pe$i (ra*ek@nensart yanedrijukn
arabe 1)
dai peiuonaf ion*nbs
smdi!h lcamb{r) se€*lame30bEebur sumbershoi!nheies
dan bak€r
nua nere*umdd
F(:HB)
d dahn serya hiso: hensos
peish*:nF(3NBIya4 d enrhhfdeh Leeert tlees)
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kdis
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lam
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b
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b
Penuunaibenrk
3am nedilm
dapar menekan
luhd Fre
petumblhai ser(sbib!ry
banyak
udd
er,
Ha ii jlea
dbnd3
less) D snpDs !,buh
menehdorsbhn mnFk kehpa sawr netud,
l1
lt
I
I
I
beBPl3HBl Pensduanrlkkb!r1r.)dan s!h! pqa hai kaG1r!):eda d8i
P13ts8)
!
Ffs dh4ibooeh Enq'€+
Biadibaidoetai deiFi
R edfopra P(3HB)yaiqdh3s
ebr (td)
dan
rh!
PeE han
P13HB) yanq
d
raioe
bd
)a
bporbn odam rar an n d percbh P(3
t,13 oc sdaiqr P13NB) y,is dMs
d
ree5r
i
menunjlkai
bdw k!3ibs
datr PI3NB) vans
dhasbn
deh
pene q,n bemr moeru P(3HB) Y:ns
Pi3HBl
tumedAr Menud.od(1es4.
membei'uhya menlad
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p*ar
pdlans pensenbaisai p(3NB)
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P(3NB) Fns
s€€
e€E btr.
feme
uh!lefuj70.c@isuhu
osac an mtur neaijllkan
pe4ouiaai
por
ner
P(3H B)
ddan bhd !@ n 3ehi+a
I
Abe H, Dd Y.Htree6)
sddraedr
d
(a.3nyd@qblrlrc kid
sh
po(3 hydoryburyEre) ,id
cordMA0s4tuymscbmsty
h@6insahsM" sa
P{ q1{o
A Maj d €0d3). Bbdesddd di
p;y6bE P(3Hs) d P(3HB.6iHV)!
/ai
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;io,me,
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oo tb
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oo . da! 0.; @, n,r.d4.
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Y dai sosa. K (leeb) N(€ar naenee
pd(rr'hydre4blryEta) aod a oFvmer or lr'
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Do Y (rseoa) Poy
F hyd@!. biodes)
4.
lcts
Prb
chaprs
Poterer
Do, Y lroeob) Mcdo€a'sn
chaore, 3 ucH Pud
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ad
Po
1re7o rhe R.e
afi Fequabr d
Efe4y
Fd! r aidDoi Y (res3r EfrcedpEdudoodpoyhydrc4aranoaEston
p:olbyAtd]genseu,,p,lsa|dl
farod al a/ n$&)
daeood.
erd (1e$b) rheRde d
NADN,nd NAoPH LnGd
A€rd,€rli
Y (ree7) Mccbiry oi bhary bends or porl(&3
hydrc4buryca.dland p0y(5) kdcacdl &1@ 33 153erss3
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3
Y
Mqd, M r
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demit curure
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(res3t rhe des€dd0o of PfB aid P(3EB/ru copoymeE
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s ai Huis z lretl
ALAIII BIOREAKTOR
I{APASITAS lOO LITER DAN PE]\iANFAATANNYA SEEACAI
akma DFma3f ' d:i aihoii Ascrei
-
bilyolEa,EsUsMab
da{hizd t c4 Fs d@md4cph,
€sae (NMR) ie hqna prcFd*
lotis we d 175 .c ft
(6c).
rH
d irc
nrb'
.c @{&r
€
(/ri, d he P€HB) sdrd vs 700 @o !
r
s
FEry
dd(rJ nd ,!r3
n4iers
d
um*an sem6
sehu:jd
(Pase. rsss)
an neryerabkai ebh dan sau
I'di.e'ewdre) shnsa
ratbD dan
rd*
6,ha €ah dbrukan
nurek
wr[n
jlb
P(3NA) di
dtai shy!
yans bdeune s
an masiesln (Lee ar
seiy*, pb3rkhd,hbruE
al
1se9
(boprtrrhdbpo*aipedamaka oeh
poims PI3EB) ie6eb
,esared,m (tui oka e,,r,1e3e) D'r
dmlai
keadaan nskh@nyaiq
eb lereklbi lesoo
P(3HB)
y,ns
eb h
r4si Pae
{oduks
*ry ,
bopa{r po(3hdoktud€u FN ha€ants d
ks (Futu
dafoo, rsesr
D
Fs€f
Md4sa
bqsE dere d6r( s eb (sbhb!
nsrunqan (bodescdsbe polmea sebasa bahai
dprc
hdokbdGrl dai behai d4 nD
|s
sery
D booadr
P'(3
ndaan bl€n
nrlii!
m
iiroprus d.
(NB).3orbhka
id,
um
rua
'
$turaohdb
n{!n rutbn a@t ltr{ @n adned Mb
dai m ny* re apa s! I betuo s
liabf
med um
i dad
d*ha*4
neiladi6 deiqan
daam
hiai n
Ebh
KzHPorsssn 1NhnztsPql
]0
mldaibrubnviamn,
b,csh dssao
ne
5
1
t
m/ lM4id
dbn lwah
d, ls*)
bah
eissorcare odia (B B€ln cedond
R&Blseama 14Fm
sobary
3
n
@
Prcduksi
13Fn
tupbstik PttHB) daan bbteaktat
dan se
ais
reagenlDramaan
vdl!
6Fm seEah
dar lee3a
r3!h $mpa 66lam smpe
roe dai Yoo reel)
dambi
P(3HB)
Fru 1ed4a
d
rebrmrya d amb r dai
Larubo
N
P13HB)
Fre bah
3e
aijlhya
P13NB)
F4 €i'd
d ddamrya
henseidap re6eb
yaklndandkdnsbi padasdu ram lDramafl
c€{edesi trhad'o sery:ft
2004)
P(3HB)
*iy,
dib,ndnsbn deruai P(3NB)
ircdan 1r
rusnetk ht (NMFJ yat
NMR
lnrk
hiimd {rukd kmb P(3HB) Ffs dhasibndenean
F4deendabnoeh ooiard (re36,
P(3HB)
(GPC) denea' pe arui
k
ororom dan
mstods
1r36b)
Fru dhtrkao dGnlbn den@n
u !k ah! ebur (derrt
$
be6r bemi mdeku (Mu). puabiomor bed moretur (Mi) seda hdeb
bn
oreh
B
ham
er,r nee)
Mqd
Prcfi
pd
reme
*,
nd iediseipada
sntu
P(3BB) yans
P(3NB)d deanboek
jam ke 43
d*lmu4rya
d
mbr
sllk dmenb4,kn r;bh Trd
Bopo (usAl sesh
bed'nFmq
!F
d
beaenbs
tunilkan
Fe
Ganb{
1
iq deiq: i safqd pe$i (ra*ek@nensart yanedrijukn
arabe 1)
dai peiuonaf ion*nbs
smdi!h lcamb{r) se€*lame30bEebur sumbershoi!nheies
dan bak€r
nua nere*umdd
F(:HB)
d dahn serya hiso: hensos
peish*:nF(3NBIya4 d enrhhfdeh Leeert tlees)
a
fi!
3"e
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5E
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e
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Me d
kdis
#
lam
(raber 1)
b
43
Grhqki €dhd
b
Penuunaibenrk
3am nedilm
dapar menekan
luhd Fre
petumblhai ser(sbib!ry
banyak
udd
er,
Ha ii jlea
dbnd3
less) D snpDs !,buh
menehdorsbhn mnFk kehpa sawr netud,
l1
lt
I
I
I
beBPl3HBl Pensduanrlkkb!r1r.)dan s!h! pqa hai kaG1r!):eda d8i
P13ts8)
!
Ffs dh4ibooeh Enq'€+
Biadibaidoetai deiFi
R edfopra P(3HB)yaiqdh3s
ebr (td)
dan
rh!
PeE han
P13HB) yanq
d
raioe
bd
)a
bporbn odam rar an n d percbh P(3
t,13 oc sdaiqr P13NB) y,is dMs
d
ree5r
i
menunjlkai
bdw k!3ibs
datr PI3NB) vans
dhasbn
deh
pene q,n bemr moeru P(3HB) Y:ns
Pi3HBl
tumedAr Menud.od(1es4.
membei'uhya menlad
tdnss ls, dtapai Fda Fm [e
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bse$ prc
k
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p*ar
pdlans pensenbaisai p(3NB)
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P(3NB) Fns
s€€
e€E btr.
feme
uh!lefuj70.c@isuhu
osac an mtur neaijllkan
pe4ouiaai
por
ner
P(3H B)
ddan bhd !@ n 3ehi+a
I
Abe H, Dd Y.Htree6)
sddraedr
d
(a.3nyd@qblrlrc kid
sh
po(3 hydoryburyEre) ,id
cordMA0s4tuymscbmsty
h@6insahsM" sa
P{ q1{o
A Maj d €0d3). Bbdesddd di
p;y6bE P(3Hs) d P(3HB.6iHV)!
/ai
1P,tn
;io,me,
Dsd€d srab 30
!o
'
.
@bbr
tF13
oo tb
Paihydnx@ka-bs.
tua
or n
fehtuq tutrusi,
e
I
1t1\:
sepb-d*
tn
e{i.hyd'oryab€i3), Jund s,hs
oo . da! 0.; @, n,r.d4.
n
D.3 dtiq D ti,rahlaagsahr
amF6 o' Fry1p4d,o4buv€e) dda
Y dai sosa. K (leeb) N(€ar naenee
pd(rr'hydre4blryEta) aod a oFvmer or lr'
hydo4blt€t) and (&hydro@EEre) itan AhataenT su@h!3 H16
Do Y (rseoa) Poy
F hyd@!. biodes)
4.
lcts
Prb
chaprs
Poterer
Do, Y lroeob) Mcdo€a'sn
chaore, 3 ucH Pud
Fotet'i
ad
Po
1re7o rhe R.e
afi Fequabr d
Efe4y
Fd! r aidDoi Y (res3r EfrcedpEdudoodpoyhydrc4aranoaEston
p:olbyAtd]genseu,,p,lsa|dl
farod al a/ n$&)
daeood.
erd (1e$b) rheRde d
NADN,nd NAoPH LnGd
A€rd,€rli
Y (ree7) Mccbiry oi bhary bends or porl(&3
hydrc4buryca.dland p0y(5) kdcacdl &1@ 33 153erss3
.oloyaneB
Y 3id oo Y
3
Y
Mqd, M r
A
(reae)
hrdrclbltEb ard 4+.^rc4b!tE€ bi rbr@as
r
11es4l
nsh
E
demit curure
or fudia,enos
(res3t rhe des€dd0o of PfB aid P(3EB/ru copoymeE
Matd MrA Hor K
thydoryh't€rc) iron Pbib o
3'dPot iqslDo Y aid FdLd3
Y (re*) PrcdEhn d Doi
iEcl
P3!e
wJ (rsst
&dera
ror{(s!pprl!r3
A lreee)
uriLdoioiPoy B ldo4bltncAcd
B
&heotd
and
eeid
c afE
F s or
F |s5rr F m,on
b
and
s{tu G (1se2r BEde4adal$doot
E tls33)
coire
B 1ree8l 83.1e'r
and
and
Erpre$m
n
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c (re!e) E{rer.i or clrre by hrbN of
asumddon pdylD
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H
ehs
1
a Ieeo) Fdmron d
kno!
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a
b
eids or udous
had!h.d3d.eiqlhro(3hydo4dh^odes);sde polner[ids trr J
Y:mene
r
1rse3).
Ybd
d
zhao
Po
s ai Huis z lretl