Fingerprinting kelapa sawit (Elaeis guinensis Jacq.) Berdasarkan Marka Simple Sequence Repeats (SSR) dan Random Amplified Polymorphism DNA (RAPD)
ABSTRAK
Arnen Pasaribu, Fingerprinting kelapa sawit (Elaeis guinensis Jacq.) Berdasarkan
Marka Simple Sequence Repeats (SSR) dan Random Amplified Polymorphism
DNA (RAPD). Dibimbing oleh Dr. Ir. Lollie Agustina P.Putri, M.Si. dan Prof. Dr.
Drs. Dwi Suryanto, M.Sc.
Kelapa sawit (E. guineensis) merupakan tanaman yang memiliki nilai
ekonomi tinggi, pengamatan tingkat molekuler perlu dilakukan untuk memberikan
informasi awal pada program persilangan tanaman. Tujuan penelitian adalah
untuk menganalisis keragaman genetik kelapa sawit dan primer yang dapat
dijadikan sebagai marker hasil fingerprinting kelapa sawit (E. guineensis) Varietas
DxP Unggul Socfindo. Analisis menggunakan marka RAPD dan SSR. Jumlah
sampel yang digunakan sebanyak 30, analisis data dilakukan dengan
menggunakan softwere Microsoft Excel 2007, Gen Alex 3.0 dan DARwin 6.0.
Kesimpulan penelitian menunjukkan hasil bahwa rataan nilai PIC marka RAPD
sebesar 0.349 dan marka SSR sebesar 0.338, marker fingerprinting teridentifikasi
pada FR-304 (120 bp) dan OPC-12 (291 bp), pita spesifik teridentifikasi pada
OPI-20 (3053 bp) dan FR-3693 (496 bp.
Kata Kunci : Kelapa sawit, SSR, RAPD, fingerprinting
Universitas Sumatera Utara
ABSTRACT
Arnen Pasaribu, Fingerprinting of oil palm (Elaeis guinensis Jacq.) based on
Simple Sequence Repeats (SSR) and Random Amplified Polymorphism DNA
(RAPD) markers. Supervised by Dr. Ir. Lollie Agustina P.Putri, M.Si. and Prof.
Dr. Drs. Dwi Suryanto, M.Sc.
Oil palm has a high economic value, evaluation in moleculer level is
needed to give first information in plant breeding program. The objective of this
research to identification about genetic variance, fingerprinting marker and
specific bands. Molecuer variance was used RAPD and SSR marker. The number
of samples waere used 30, Microsoft Excel 2007, Gen Alex 3.0 and DARwin 6.0
were used. to analysis of the data The conclusions of this research showed that
means of PIC were 0.349 for RAPD marker and 0.338 for SSR marker,
fingerprinting markers were identification on FR-304 (120 bp) and OPC-12 (291
bp), specific bands were identification on OPI-20 (3053 bp) and FR-3693 (496
bp).
Keywords : Oil palm, SSR, RAPD, fingerprinting
Universitas Sumatera Utara
Arnen Pasaribu, Fingerprinting kelapa sawit (Elaeis guinensis Jacq.) Berdasarkan
Marka Simple Sequence Repeats (SSR) dan Random Amplified Polymorphism
DNA (RAPD). Dibimbing oleh Dr. Ir. Lollie Agustina P.Putri, M.Si. dan Prof. Dr.
Drs. Dwi Suryanto, M.Sc.
Kelapa sawit (E. guineensis) merupakan tanaman yang memiliki nilai
ekonomi tinggi, pengamatan tingkat molekuler perlu dilakukan untuk memberikan
informasi awal pada program persilangan tanaman. Tujuan penelitian adalah
untuk menganalisis keragaman genetik kelapa sawit dan primer yang dapat
dijadikan sebagai marker hasil fingerprinting kelapa sawit (E. guineensis) Varietas
DxP Unggul Socfindo. Analisis menggunakan marka RAPD dan SSR. Jumlah
sampel yang digunakan sebanyak 30, analisis data dilakukan dengan
menggunakan softwere Microsoft Excel 2007, Gen Alex 3.0 dan DARwin 6.0.
Kesimpulan penelitian menunjukkan hasil bahwa rataan nilai PIC marka RAPD
sebesar 0.349 dan marka SSR sebesar 0.338, marker fingerprinting teridentifikasi
pada FR-304 (120 bp) dan OPC-12 (291 bp), pita spesifik teridentifikasi pada
OPI-20 (3053 bp) dan FR-3693 (496 bp.
Kata Kunci : Kelapa sawit, SSR, RAPD, fingerprinting
Universitas Sumatera Utara
ABSTRACT
Arnen Pasaribu, Fingerprinting of oil palm (Elaeis guinensis Jacq.) based on
Simple Sequence Repeats (SSR) and Random Amplified Polymorphism DNA
(RAPD) markers. Supervised by Dr. Ir. Lollie Agustina P.Putri, M.Si. and Prof.
Dr. Drs. Dwi Suryanto, M.Sc.
Oil palm has a high economic value, evaluation in moleculer level is
needed to give first information in plant breeding program. The objective of this
research to identification about genetic variance, fingerprinting marker and
specific bands. Molecuer variance was used RAPD and SSR marker. The number
of samples waere used 30, Microsoft Excel 2007, Gen Alex 3.0 and DARwin 6.0
were used. to analysis of the data The conclusions of this research showed that
means of PIC were 0.349 for RAPD marker and 0.338 for SSR marker,
fingerprinting markers were identification on FR-304 (120 bp) and OPC-12 (291
bp), specific bands were identification on OPI-20 (3053 bp) and FR-3693 (496
bp).
Keywords : Oil palm, SSR, RAPD, fingerprinting
Universitas Sumatera Utara