Effect of Diabetes Condition on Topical Treatment of Binahong Leaf Fraction in Wound Healing Process | Kintoko | Majalah Obat Tradisional (Traditional Medicine Journal) 27921 61651 1 PB
Submitted : 02-12-2016
Revised : 25-05-2017
Accepted : 14-06-2017
Trad. Med. J., May - August 2017
Vol. 22(2), p 103-110
ISSN-p : 1410-5918 ISSN-e : 2406-9086
Effect of Diabetes Condition on Topical Treatment of Binahong Leaf
Fraction in Wound Healing Process
Pengaruh Kondisi Diabetes pada Pemberian Topikal Fraksi Daun Binahong dalam
Proses Penyembuhan Luka
Kintoko1*, Hanifah Karimatulhajj2, Trie Yuni Elfasyari2, Ersi Arviana Ihsan2, Teguh Adiyas Putra2,
Puspawan Hariadi2, Citra Ariani1 , Nurkhasanah1
1Fakultas
Farmasi, Universitas Ahmad Dahlan. Yogyakarta
Farmasi, Universitas Ahmad Dahlan, Yogyakarta
2Pascasarjana
ABSTRACT
Diabetes mellitus (DM) is a metabolic disease which is becoming the first number of health problem in
Indonesia, based on the results of the Basic Health Research of Ministry of Health in 2013. One of the diabetes
complications affected by high levels of blood glucose is diabetic ulcers wich 85% the number of cases was
overed by amputation as the result of improper handling. Herbal treatments could be an alternative
treatment of diabetic ulcers, one of them is binahong plant. The results of previous studies have shown the
ability of ethanolic extract of binahong leaf in accelerating wound healing in diabetic rats. This study would
be a continued study to test five kinds of binahong leaf fractions (FDB) with gradual solvent polarity in
accelerating wound healing in diabetic ulcer. Wound healing parameters observed were percentage of
wound healing from the wound diameter contraction. The test begins with ethanolic extract of binahong leaf
fractionation to produce fractions of hexane (FHDB), chloroform (FKDB), ethyl acetate (FEADB), and ethanol
(FEDB). Water fraction (FADB) was obtained from ethanolic leaf extract residues of binahong leaf extraction.
Each fraction activity was tested topically twice daily on dorsal of test animals that created wounds using a
punch biopsy 5 mm diameters. Grouping of test animals were divided into 13 groups with normal control
group (non diabetes), negative control (diabetes + topical application of biocream®), positive control
(diabetes + topical application of madecassol), and 10 diabetes groups with topical application of 5 kinds of
binahong leaf fractions with each fractions consist of a concentrations of 5% and 10% with biocream® as a
vehicle. Diabetic parameters measured include blood glucose levels (KGD) and weight lost percentage (PB%)
in day 1 and 10 during a 10-day treatment. The results showed the influence of KGD in the condition of
diabetes on wound healing rats diabetic ulcers which topically treated with binahong leaf fractions. FADB
10% has significant differences potention in wound healing process in diabetic rats macroscopically that
accelerates wound contraction compared with negative and positive control groups (p
Revised : 25-05-2017
Accepted : 14-06-2017
Trad. Med. J., May - August 2017
Vol. 22(2), p 103-110
ISSN-p : 1410-5918 ISSN-e : 2406-9086
Effect of Diabetes Condition on Topical Treatment of Binahong Leaf
Fraction in Wound Healing Process
Pengaruh Kondisi Diabetes pada Pemberian Topikal Fraksi Daun Binahong dalam
Proses Penyembuhan Luka
Kintoko1*, Hanifah Karimatulhajj2, Trie Yuni Elfasyari2, Ersi Arviana Ihsan2, Teguh Adiyas Putra2,
Puspawan Hariadi2, Citra Ariani1 , Nurkhasanah1
1Fakultas
Farmasi, Universitas Ahmad Dahlan. Yogyakarta
Farmasi, Universitas Ahmad Dahlan, Yogyakarta
2Pascasarjana
ABSTRACT
Diabetes mellitus (DM) is a metabolic disease which is becoming the first number of health problem in
Indonesia, based on the results of the Basic Health Research of Ministry of Health in 2013. One of the diabetes
complications affected by high levels of blood glucose is diabetic ulcers wich 85% the number of cases was
overed by amputation as the result of improper handling. Herbal treatments could be an alternative
treatment of diabetic ulcers, one of them is binahong plant. The results of previous studies have shown the
ability of ethanolic extract of binahong leaf in accelerating wound healing in diabetic rats. This study would
be a continued study to test five kinds of binahong leaf fractions (FDB) with gradual solvent polarity in
accelerating wound healing in diabetic ulcer. Wound healing parameters observed were percentage of
wound healing from the wound diameter contraction. The test begins with ethanolic extract of binahong leaf
fractionation to produce fractions of hexane (FHDB), chloroform (FKDB), ethyl acetate (FEADB), and ethanol
(FEDB). Water fraction (FADB) was obtained from ethanolic leaf extract residues of binahong leaf extraction.
Each fraction activity was tested topically twice daily on dorsal of test animals that created wounds using a
punch biopsy 5 mm diameters. Grouping of test animals were divided into 13 groups with normal control
group (non diabetes), negative control (diabetes + topical application of biocream®), positive control
(diabetes + topical application of madecassol), and 10 diabetes groups with topical application of 5 kinds of
binahong leaf fractions with each fractions consist of a concentrations of 5% and 10% with biocream® as a
vehicle. Diabetic parameters measured include blood glucose levels (KGD) and weight lost percentage (PB%)
in day 1 and 10 during a 10-day treatment. The results showed the influence of KGD in the condition of
diabetes on wound healing rats diabetic ulcers which topically treated with binahong leaf fractions. FADB
10% has significant differences potention in wound healing process in diabetic rats macroscopically that
accelerates wound contraction compared with negative and positive control groups (p