Pengaruh Beberapa Jenis Pakan Terhadap Pertumbuhan Rotifera (Brachionus sp)

LAMPIRAN

Universitas Sumatera Utara

1. Alat dan Bahan
Alat

Botol Ukuran 250 ml

Mikroskop Cahaya

Aerator

Bohlam 5 Watt

Haemocytometer

Akuarium Mini

Universitas Sumatera Utara


Termometer

Corong

Pipet Tetes

pH Meter

Tisu

Jarum Suntik

Universitas Sumatera Utara

Kertas Label

Spidol

Bahan


Vitamin B Kompleks Cair

Ragi Roti

Ampas Tahu

Universitas Sumatera Utara

Aquadest

Alkohol 70 %

Universitas Sumatera Utara

2.

Prosedur Penelitian

Bibit dari Alam


Proses Pemasukan Bibit
Yang Sudah Dikultur

Pemberian Pakan Ragi Roti dan Vitamin B Kompleks

Universitas Sumatera Utara

Media Uji

Tampilan Media Siap Untuk Diuji

Universitas Sumatera Utara

3.

Rotifera (Brachionus sp)

Universitas Sumatera Utara

4. Tabel Pengamatan Pertumbuhan Harian Brachionus sp

Perlakuan

A (Fitoplankton)

B (Ampas Tahu)

C (Ragi Roti)

D (Vit. B Kompleks)

Pengamatan

Ulangan

1
2
3
Jumlah
Rata-rata
1

2
3
Jumlah
Rata-rata
1
2
3
Jumlah
Rata-rata
1
2
3
Jumlah
Rata-rata

Awal

1 (Hari ke-2)

2 (Hari ke-4)


3 (Hari k3-6)

4 (Hari k3-8)

5 (Hari ke-10)

0.001
0.001
0.001
0.003
0.001
0.001
0.001
0.001
0.003
0.001
0.001
0.001
0.001

0.003
0.001
0.001
0.001
0.001
0.003
0.001

2
1
1
4
1.33
2
3
3
8
2.67
1
0.3

0.3
1.6
0.53
2
2
2
6
2.00

2
1
1
4
1.33
12
15
10
37
12.33
1

0.3
1
2.3
0.77
15
25
10
50
16.67

2
1
2
5
1.67
22
16
15
53
17.67

5
1
1
7
2.33
5
5
5
15
5.00

2
2
2
6
2.00
60
18
25
103

34.33
40
30
20
90
30.00
3
5
80
88
29.33

1
1
1
3
1.00
50
15
20
85
28.33
30
20
15
65
21.67
2
2
60
64
21.33

Universitas Sumatera Utara

5. Tabel Hasil Pertumbuhan Populasi dan Pertumbuhan Harian Brachionus
sp pada Perlakuan

Pertumbuhan Populasi Brachionus sp
Perlakuan

I
8
96
47
25

A (Fitoplankton)
B ( Ampas Tahu)
C ( Ragi Roti)
D ( Vit. B Kompleks )

Ulangan
II
III
5
6
52
53
31.6
22.3
37
97

Jumlah

Rata-rata

19
201
100.9
159

6.33
67
33.63
53

Pertumbuhan Harian Populasi Brachionus sp Pada Setiap Perlakuan.
Media Perlakuan

Waktu Pengamatan
Fitoplankton

Ampas Tahu

Ragi Roti

Vitamin B Kompleks

Hari Pertama

0.001

0.001

0.001

0.001

Hari Ke-2

1.33

2.67

0.53

2.00

Hari Ke-4

1.33

12.33

0.77

16.67

Hari Ke-6

1.67

17.67

2.33

5.00

Hari Ke-8

2.00

34.33

30.00

29.33

Hari ke 10

1.00

18.33

15.00

9.66

Universitas Sumatera Utara

6. Tabel Analisis Anova Sidik Ragam Pertumbuhan Populasi Rotifera
(Brachionus sp)

Perlakuan
A (Fitoplankton)
B ( Ampas Tahu)
C ( Ragi Roti)
D ( Vit. B Kompleks )
Jumlah
Rata-rata

I
8
96
47
25
176
44

Ulangan
II
5
52
31.6
37
125.6
31.4

III
6
53
22.3
97
178.3
44.575





19
201
100.9
159
479.9

6.33
67
33.63
53
39.99

DB Total

= Jumlah seluruh observasi- 1 = n – 1 = 12 – 1 = 11

DB Perlakuan

= Banyaknya perlakuan – 1 = 4 – 1 = 3

DB Galat

= DB total - DB perlakuan
= t (r –1) = (Jumlah perlakuan) (jumlah ulangan – 1)
= 11 – 3 = 8

Faktor Koreksi (FK)

= (GT)2 = (479,9)2 = 230304,01 = 19192,00
n
12
12

JK Total

= (n-observasi total )2 – FK
= {(8)2 + (5)2 + (6)2 + (96)2 + (52)2 + (53)2 + (47)2 +
(31,6)2

+(22,3)2 +(25)2+ (37)2 + (97)2 } –

19192,00
= 10769,85

JK Perlakuan

= ∑ (total perlakuan)2 – FK
r
= (19)2 + (201)2 + (100.9)2 + (159)2 – 19192,00
3
= 6215,9366

Universitas Sumatera Utara

JK Galat

= JK Total – JK Perlakuan
= 10769,85 – 6215,9366
= 4553,91

KT Perlakuan

= JK Perlakuan = 6215,9366 = 2071.9788
DB Perlakuan
3

KT Galat

= JK Galat = 4553,91 = 569.2387
T (r - 1)
8

F Hitung Perlakuan

= KT Perlakuan = 2071.9788
KT Galat

F Hitung Ulangan

= KT Ulangan
KT Galat

JK

= 221.78083

Sumber Variasi

DB

KT

PERLAKUAN

3

6215.9366 2071.9788

ULANGAN
GALAT
TOTAL

2
8
11

443.56167 221.78083
4553.91
569.2387
10769.85

F hit
3.63991204
0.38960954

F tabel
5%
4.76

Universitas Sumatera Utara

1%
9.78

7. Hasil Analisis Anova Menggunakan Program SPSS 17

Correlations
Notes
Output Created

20-Nov-2013 09:50:19

Comments
Input

Active Dataset

DataSet0er

Filter



Weight



Split File



N of Rows in Working Data File
Missing Value Handling

Definition of Missing

4
User-defined missing values are treated as
missing.

Cases Used

Statistics for each pair of variables are based
on all the cases with valid data for that pair.

Syntax

CORRELATIONS
/VARIABLES=Fitoplankton AmpasTahu
RagiRoti VitaminBKompleks
/PRINT=TWOTAIL NOSIG
/STATISTICS DESCRIPTIVES
/MISSING=PAIRWISE.

Resources

Processor Time

0:00:00.000

Elapsed Time

0:00:00.032

[DataSet0]
Descriptive Statistics
Mean

Std. Deviation

N

Fitoplankton

6.33

1.528

3

Ampas Tahu

67.00

25.120

3

Ragi Roti

33.633

12.4749

3

Vit. B Kompleks

53.000

38.5746

3

Universitas Sumatera Utara

Correlations
Fitoplankton
Fitoplankton

Pearson Correlation

Ampas Tahu
1

.755

-.339

.200

.455

.780

3

3

3

3

Pearson Correlation

.951

1

.920

-.613

Sig. (2-tailed)

.200

.256

.580

N

N
Ragi Roti

3

3

3

3

Pearson Correlation

.755

.920

1

-.873

Sig. (2-tailed)

.455

.256

3

3

3

3

-.339

-.613

-.873

1

.780

.580

.324

3

3

3

N
Vit. B Kompleks

Pearson Correlation
Sig. (2-tailed)
N

REGRESSION
LISTWISE

Vit. B Kompleks

.951

Sig. (2-tailed)

Ampas Tahu

Ragi Roti

/DESCRIPTIVES MEAN STDDEV CORR SIG N

.324

/MISSING

/STATISTICS COEFF OUTS R ANOVA COLLIN TOL CHANGE ZPP

/CRITERIA=PIN(.05) POUT(.10)

/NOORIGIN

/DEPENDENT Fitoplankton

/METHOD=ENTER AmpasTahu RagiRoti VitaminBKompleks

/RESIDUALS

HIST(ZRESID) NORM(ZRESID).

Universitas Sumatera Utara

3

Regression

Notes
Output Created

20-Nov-2013 09:51:15

Comments
Input

Active Dataset

DataSet0

Filter



Weight



Split File



N of Rows in Working Data File
Missing Value Handling

Definition of Missing

4
User-defined missing values are treated as
missing.

Cases Used

Statistics are based on cases with no
missing values for any variable used.

Syntax

REGRESSION
/DESCRIPTIVES MEAN STDDEV CORR
SIG N
/MISSING LISTWISE
/STATISTICS COEFF OUTS R ANOVA
COLLIN TOL CHANGE ZPP
/CRITERIA=PIN(.05) POUT(.10)
/NOORIGIN
/DEPENDENT Fitoplankton
/METHOD=ENTER AmpasTahu RagiRoti
VitaminBKompleks
/RESIDUALS HIST(ZRESID)
NORM(ZRESID).

Resources

Processor Time

0:00:00.063

Elapsed Time

0:00:00.077

Memory Required
Additional Memory Required for

1956 bytes
640 bytes

Residual Plots

[DataSet

Universitas Sumatera Utara

Warnings
For the final model with dependent variable Fitoplankton, the variance- covariance matrix is singular.
Influence statistics cannot be computed.

Descriptive Statistics
Mean

Std. Deviation

N

Fitoplankton

6.33

1.528

3

Ampas Tahu

67.00

25.120

3

Ragi Roti

33.633

12.4749

3

Vit. B Kompleks

53.000

38.5746

3

Correlations
Fitoplankton
Pearson Correlation

N

Ragi Roti

Vit. B Kompleks

Fitoplankton

1.000

.951

.755

-.339

Ampas Tahu

.951

1.000

.920

-.613

Ragi Roti

.755

.920

1.000

-.873

-.339

-.613

-.873

1.000

Fitoplankton

.

.100

.228

.390

Ampas Tahu

.100

.

.128

.290

Ragi Roti

.228

.128

.

.162

Vit. B Kompleks

.390

.290

.162

.

Fitoplankton

3

3

3

3

Ampas Tahu

3

3

3

3

Ragi Roti

3

3

3

3

Vit. B Kompleks

3

3

3

3

Vit. B Kompleks
Sig. (1-tailed)

Ampas Tahu

Universitas Sumatera Utara

Model Summaryb
Change Statistics

Std. Error of the
Model

R

R Square

1.000a

1

Adjusted R Square

1.000

Estimate

.

R Square Change
.

1.000

F Change

df1
.

df2
2

Sig. F Change
0

.

a. Predictors: (Constant), Vit. B Kompleks, Ampas Tahu
b. Dependent Variable: Fitoplankton
Model
1

Variables Entered Variables Removed
Vit. B Kompleks,

Method

. Enter

a

Ampas Tahu

a. Tolerance = .000 limits reached.

Universitas Sumatera Utara

ANOVAb
Model
1

Sum of Squares
Regression
Residual
Total

df

Mean Square

F

4.667

2

2.333

.000

0

.

4.667

2

Sig.
.a

.

a. Predictors: (Constant), Vit. B Kompleks, Ampas Tahu
b. Dependent Variable: Fitoplankton

Coefficientsa
Standardized
Unstandardized Coefficients
Model

B

1(Constant)

Coefficients

Std. Error

Correlations

Beta

.663

.000

Ampas Tahu

.072

.000

Vit. B Kompleks

.015

.000

t

Sig.

Zero-order

Partial

Collinearity Statistics
Part

Tolerance

VIF

.

.

1.191

.

.

.951

1.000

.941

.624

1.602

.390

.

.

-.339

1.000

.308

.624

1.602

a. Dependent Variable: Fitoplankton

Universitas Sumatera Utara

Excluded Variablesb
Collinearity Statistics
Minimum
Model
1

Beta In

t
.a

Ragi Roti

Sig.
.

Partial Correlation
.

Tolerance
.

VIF

.000

Tolerance
.

.000

a. Predictors in the Model: (Constant), Vit. B Kompleks, Ampas Tahu
b. Dependent Variable: Fitoplankton

Collinearity Diagnosticsa
Variance Proportions

Dimensio
Model

n

Eigenvalue

Condition Index

(Constant)

Ampas Tahu

Vit. B Kompleks

1

1

2.701

1.000

.00

.01

.02

2

.281

3.101

.00

.07

.36

3

.018

12.114

.99

.93

.62

a. Dependent Variable: Fitoplankton

Universitas Sumatera Utara

Residuals Statisticsa
Minimum

Maximum

Mean

Std. Deviation

N

Predicted Value

5.00

8.00

6.33

1.528

3

Residual

.000

.000

.000

.000

3

-.873

1.091

.000

1.000

3

.

.

.

.

0

Std. Predicted Value
Std. Residual
a. Dependent Variable: Fitoplankton

Universitas Sumatera Utara

Universitas Sumatera Utara