Molecular Mechanism of Synthesized Potential Anticancer Agent Chalcone in Leukemia Cell Line K562 | Novilla | Journal of the Medical Sciences (Berkala ilmu Kedokteran) 17777 75410 1 PB
Novilla
et al.,
of cytotoxic
J Med Sci, Volume
49, No.
4, Mechanism
2017 October:
153-164 activity of chalcone derivatives against K562 leukemia cell lines
Mechanism of cytotoxic activity of
chalcone derivatives against K562
leukemia cell lines
Arina Novilla,1,2*, Indwiani Astuti3, Jumina4, Hery Suwito5, Mustofa3
1
Doctoral Program of Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta,
2
Department of Medical Laboratory Technology, School of Health Sciences Jenderal
Achmad Yani, Cimahi, 3Department of Pharmacology and Therapy, Faculty of Medicine,
Universitas Gadjah Mada, Yogyakarta, 4Department of Chemistry, Faculty of Mathematics
and Natural Sciences, Universitas Gadjah Mada, Yogyakarta, 5Department of Biology,
Faculty of Science and Technology, Airlangga University, Surabaya, Indonesia
DOI: http://dx.doi.org/10.19106/JMedSci004904201701
ABSTRACT
Two chalcone derivatives i.e. (E)-1-(4-aminophenyl)-3-(2,3dimethoxyphenyl)-prop-2-en-1one (Compound-1), and (E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one) (Compound-2),
has been proven to have potential cytotoxic activity. The aim of this study was to
evaluate the effect of these compounds on PI3K/Akt signalling pathway in K562 cell
lines. After incubation with the tested compounds, AKT, caspase-3, STAT3 and cyclin
D1 concentrations were measured using ELISA. Furthermore, cell cycle was analysed
using lowcytometry. Imatinib and isotretinoin were used as positive control, whereas
cell culture without treatment was used as negative control. The AKT concentration after
treatment with Compound-1 and -2 was signiicantly lower than that control, imatinib
and isotretinoin (p
et al.,
of cytotoxic
J Med Sci, Volume
49, No.
4, Mechanism
2017 October:
153-164 activity of chalcone derivatives against K562 leukemia cell lines
Mechanism of cytotoxic activity of
chalcone derivatives against K562
leukemia cell lines
Arina Novilla,1,2*, Indwiani Astuti3, Jumina4, Hery Suwito5, Mustofa3
1
Doctoral Program of Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta,
2
Department of Medical Laboratory Technology, School of Health Sciences Jenderal
Achmad Yani, Cimahi, 3Department of Pharmacology and Therapy, Faculty of Medicine,
Universitas Gadjah Mada, Yogyakarta, 4Department of Chemistry, Faculty of Mathematics
and Natural Sciences, Universitas Gadjah Mada, Yogyakarta, 5Department of Biology,
Faculty of Science and Technology, Airlangga University, Surabaya, Indonesia
DOI: http://dx.doi.org/10.19106/JMedSci004904201701
ABSTRACT
Two chalcone derivatives i.e. (E)-1-(4-aminophenyl)-3-(2,3dimethoxyphenyl)-prop-2-en-1one (Compound-1), and (E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one) (Compound-2),
has been proven to have potential cytotoxic activity. The aim of this study was to
evaluate the effect of these compounds on PI3K/Akt signalling pathway in K562 cell
lines. After incubation with the tested compounds, AKT, caspase-3, STAT3 and cyclin
D1 concentrations were measured using ELISA. Furthermore, cell cycle was analysed
using lowcytometry. Imatinib and isotretinoin were used as positive control, whereas
cell culture without treatment was used as negative control. The AKT concentration after
treatment with Compound-1 and -2 was signiicantly lower than that control, imatinib
and isotretinoin (p