PENGARUH LAMA WAKTU TRANSPORTASI SETELAH PEMOTONGAN TERHADAP STATUS INTI OOSIT SAPI YANG DIMATANGKAN SECARA IN-VITRO.
11||MNSP0R tASMTEL^H pl.Nil)|1)NCAN
^K
lrs rN1ll)(Jstts'\Pt
YANri otill]\TANcK,\NSt(.AR^ / ' r,77F'
r.ANr'\ u
(rleh lJ.lbcn
Sani
iDjbrsrhhin$nlai l,rulD.l..ll strr.diMs..r,tsdirjDr.t..lt. Jayaodi.llls)
RN(IIi,\SAN
liOa)a mcningkrtlan ptuduhivnrs sini mcLtrlui ncmr3|]ln r.tDdlosi trdiist ,l
=btr!.L rllemarilF.dLk! ed)i.drl. i nchk$nIi tDdit'iraptrnNan0unr.riulJ.lr
:.::_trlxn
ltxtuh lanN lel]ih ine0eutrtui-jliu lsknotogi l.(il\r\i ,r rr/, Nnrp.k.
-
-.:: r!i
(trt,kprontr[ri eDibri.t]dali0!Lurgm bLrr2n (di t!dr'btrh)
P. .lirirn inl b€nulurtr trrtrk Drcigdrhri psng:ruh ta r \ratru hns0otusircdrdrp
::.r l.tr raigrn..ri( srf. n,errcr.htri pcns.uh .lalir! hn\omsi terhsdop Itrgkd
':. 15ilosir \api. (r,An lanq DernuiJ-ri b.bc.apr lrfnscttumulL'r k.m|]ak d nrar.dgk.n
s::-: ,, rrfu nengelrakaD nfdiu JiM r9r) )-rtre disrpLcmcnrrsi denSri t r./.
- :,..rrr! r/l,/,o,. (lSI l) l0 rg/ml.s.rum saf 0t; dxn grnrrnisi'r 50 lL!htpdrca\rn
t: ori nrnl) !! anr 11.iir p.di suh! 13.5!c d:hn irktrtixlor tur i9:o
P(nrlurn adrah I Frlode hnrx \xlrL ransf.ft.s .rarn'm rairl l jan. 7jxm. r0
r! l i ro lr
. , J:h...J
.,{o,(t.t.
,".n
'
,n' i
'' lJ't'
.- n
'-.,
i:: nni Ltrrlir! olril urluk s.riip pcrhLnn Dr.ftrduttrn p3rurrDar kurtilr\..ri(
!e-rtif
:._:.i b. rmbxhiyr hDr \!rli' hn\0.nrsi srjd d n.rotr! Ljcolln ra esiir di
-. -ri!L[.ti pcrh.duf ] r! trrrrn (P.1051 x . bc.hrsaj i.rtikurn trn \rltu
:r_ii\ri\i. Tinlhl kciubnlad re trlgiprdr*2knr rn sponrjiljxn yairr srbAar j6 25
1r_ !trf! rctundrh nrdr D(lakuali \.[rr rnLnroodrs ]tjim!irtrseb.rar262j Oosn!rDg
:r. nr.n.rpri hhrf D,di0g ar.n Nlll dr|r]r rcrruli prda hcdDlaj thap scbc trnnya
::!ricrmlind \k!cr. ((lv). Gc.m ml veri.l. I]..itdo\m ((jvrD), Mclrlisc I. frfik
r T.l.,[]!c I l'.i\ctru* oori lc.linili\asi -,rr! LLcnsan n.isguiakan scmcn el]i dari
... rrn! borsrl diri scrrcn roi \i'neirrt \irig LtipfudLtsi .ch BtB Turh Srtrb
:.-rirmbuh Rrli rdi psscn{ric losirrang r..r'cnrli:rsi
:
-.
lcdins-!i diFro ct pad. p.rtrIun
trtrsponasi ,l .r.rh r,rtu lcbt\ar 6t 62 dad lc.rndxh orda p$aLran tan \raLtr,
ir rrnu scl,c$r ll50. Pchilrs. ..sn reftini issi padr bcha|aip.rL.Irn lanuN]kru
:\'nr!i .rirnn s.b.gni !'mhc. oo\ir trrftrlirkrn rd]nrr n.rtrclt n ]rng \rngrt i)rh
: rrlltr
ri.r.rlr.riLro linili:Aioor
r
t)rJ3liN trrLtr hD!Nnari 10-ll
l.bih
iaor
fur
: :rh drrl lior \rlru I m mrfunirlki brh\yr ovrlnrn d,rsih mdnl)Url.j
torcdli
- :ji!$,..r oo!1rnrul p JuL\ enlnlo i !irnr r)r|d disihprt[xn hah$] trDa N.krr
, .:idrsjnchprngrn,hjrjrbl[\difkor\tr1cnri..\nrftk lrrnsltam neDiiaicii tui
oresk
I. PEI{D,/\' II]I,IJAN
l.n\ep |EDbanlud.n p.(cmx[rn adrlrh Dc\{iudkrn k.nd( o.rlh.tan }rD{
-rjtr. .fisicn dxi rxnlgrh Irrg
.ri\i k
dicnikan ol.h
. lc'nxmnun Drenles ai[an
:f,*r scd.
Jrlam
-:.mxlxn
--:-sulan
N]. du {nkrur
|ioduksj dcnlxn
tehuruhai
tcmi ai
Lcmaml]ufDya untuk nc )unbdig re.hadap pcmbrngtrnrn qilavilr.
!*mpdxn [er[ peid3Fhi dxi
!T.n
lcmampuiflr memcnlhi
CcrbiilaD
rrrl
hiduC, pcrbritan ]ineturgir,
tedNrhlhr ek.noni. l\lrnrir scbalai pcngge.r(
Dre',jad
htr |.rc,mk.tr !.brgri irdunri
hioLosis
lcnl
t.nrtr.dgunrd
hirgga ncfjodj tomodirrs
\cdr mdnr)Lr)ai niri ko icrsirliings Khusus unru[ rer k epidiDituxnq
::rpunrlipcr
slrrns.uktrc rrc*rdlhm mctrirgkalkai k.rrhrnan FD3xn. khur'snyr
::r:: l,clnni. nefiir.qliatk klrl1r5 ttsrn' d.u
-:. ig[.rl|n
::!r1 Dila
n ndrnrLn drD krs.j.r,rsrln nrsrr!kar
nrbsirtr\i imo.r. sckxljstrj
hctaLLLi
ncmh€.d]txai ckononrj
usrhr Dremnc.ftia( rju ptuduk\i ccrcft'rlln letrh diti(trkrn bcn]rlri
-:it3 h,,il \cctrru refdekrirf klrilnrril d.ngrr pcrn{ldxn popu]$i rcnrI drr
:.l.i:\diri ILrlnrl]f |tD f.Lkrtri nrodrl5l ne. unir ic mk dcngrn pr.{rn tiscnrj,rlj
l.i
llBl. Ttun\ler
l\nhri(rlr)diii VcdilisrsirJ,!r, (ftV)
UprJl Dmingkrlkrn pFdukiivilrr srni nchLi pctrc..pxn lc[no.gi t enitn.si,]
i.
.
Scbrlri r t.nrr
I rodtrlsi
cmbrio
drhfr nct.l,sarran Tl: dihrrCkaf
mrnipn
trig tbir Drcretrtrrn![rn. ncmbuti] p!tu.irr trtrr,tr
-:-.]!mbaig[rr t.kfk irtriJr]lsi!r ddaf !nb.io. tloni'ig. raNlci k. kjmen d j
-:_:rb.'1, pclcmxlrtr k.rrrr
ir-:n!i-!cnrlik(a;dd.r.9q1).Tc[noLosilentisi,rr/r,mcrucrr]irck!togirnr,lr
-;u[ii.mh.o|trdr ]iqk0 F. budntd lL{rfuljrht.
irr. r,{ fircfttixlr\:.tungLrirD [c!iki ]ris mcLiptrr Fnorif!ii
'
.:1. !in !p.mrbToadrn
krltu, cmtiio.
oo5ir.
ftnile!
Bebenpr |]tnelirirn poduk\l ernhio scc..a
refluk scpcftl sipi
(l
r/,
rcunsoi (r
199,1
dln
z rrlm lchh
ditaporkan padi b.rbrgri
r/., 2001). lterhau niey zt
BocdioDo ?!
,lls9l).do'nba(Bro{n./r/.,l993.drnla*\rndi.rrl.,200t).tambing(pr*srrc.rd,
199,1)
Janpadrkucins.rniinsdancheehh(Bevc.idg.danJabi.u..
In,Jtr[sicnbrtu,
Dr am
d norone
i?rD
o.sir r-rng dilunaltln
di RPll (Runiah Po.org Il.N.n)
dip.rolch n.laluircknLk
rl.
,
'nlrah
Lomplck. Kcddtrlr
dri
nc
b.nlur Lllnrah.tnf) I.thl d
l{flt Dr.ruFtar
di|c6lch dxri
sumber
I 'jjrn
rr!,
rlV
ooslr dari he\aD
Nlenu.u( ihne
sc(chh ncmobigan
naisponasi
rrnuksl eirhrio ,r
lcbih mrhal dai mcmblr'hkxn psrtahn ratrB cbih
ri li.r.n
RPll dcngan Lrbo.nroriLLm
rp.di \vilN
an8
dari hcrqn hidrp lxng
did teAcdia lal.m junnrh han)rk. scdrigltrtr rxns dil](o.h de4r.
r.\rik arrr,, trt ,ir cl.ril
Jtrlrir \r.kn,
dqd bensatdrrisapi ya !
xri di petul.h
(Ol'Ll) d.ngrn
Koch.r././.,20021 Oorit
1999 dxn
msn !an!
Oryr,lnr U/
I993).
.lu'
.r
)'tr.
diCDl.ns adrhh
!1..(200:l)
orrriun
irrrt arrrn
harus dikot!ksi
Peniis(dr xrltt' lsjak rrc\str
nrcntrrun[rn
v]rlilnrs oorir
raDS
rcnnasutrxhap ncn ratrsaf doi r.nilisasirrr
diNrodg
dilunxtaf um'k
rirn, Rcdasartaf
.iparai di rtat !fr!L D,ctrlcuhui lxmr *r[ltr rxnsp.turi .\r.iuD $0i ftrh
\m.ron?ri sanpri lc hbo{rornim FIV )rDS tcrbrik dxn
Drsfda|alkai rtrlko
-mdafgrd oosn )_rd! ri,r!sL mrka nJntr!s tr,.Lrkuk. pcncliliai d.rgrD jLldut
Pcigrrun
Lrhr \txklu T..nspodxsis.icrah
ilpirrog
di
M|t,nqrxn Sccrm r, t ?rr.
F.Rordngrn tcrhrd3n
:
5lr(6 t riOosi.
V, KESTMPULAN DAN SARAN
Bederkan h.sil dri
r
penelirian inimak!dap.r
di6rik bcbcrjpr kesinrputan:
l-an!rvaku tanspotusi menpongrruhj viabitiras
hcngalami pemarangan nri d.n fe.lilisasi,
b.
Pe6enrac pemalaq. n
PcMn rase
oos ir
narang
m en uru
un
n dcngan
datr komperensi oosit unruk
rrro
senatin lrmanya
rlk waktr' ran sporhsi
\yakr u
rrn
sponas i.
da. Bja,nnEsing
4. 7, t0
frasins adalah 56.25. 46.25. 31.75 dan 26 25%
c. P.nenbse @sir
tertijniiisrsi mciurun detrgan senrakin tafranla lvaktu tnnspotusi.
Pe6enraseoosnrcrlcnilissi unlukwakturansponlsi,l,T, t0drn
masing aditah 62.62.
,13 6,1,
44.79 dan
tjjindasing
t2 50%
Bc.dasarkar hasil pcnclirirn dan kesi'npulai drtam penetiri{
inj n:ka
dapd
dikcrukanm bcberapa s..aD kbr8 ibo.ikur.
a. Un(uk mcdap.tkan penenEsD o.sir marang yafg r$baik
harus dikoleksi
osiinyr datafr *ak!u
ktrrang dari 4
*.a
jrm, ufruk msjnperlah.r
!i$ilibsdarkompcrensioostrdnluIfcrlitisasiryrropcrLLkajiar].bihlrnjui
b PerlukrjarlcbihLnjtrlie
pe;kernbancan
.mbri. lr
ada
'/'r,.
p pengrnrh
l.m
wa(to hn
spo das i
1.d.d ap
Ailotro
T.l.L. SlhN.az, N.L fner Md M.l-.l.eibaried,Ruttedec. 1996. AsOdclr oftDlti!1.
aid $agc rhar aflcsr
Itro maru.arioi and dcvclopmcnr ol boline oflcs
',
Therioreidlosy 45:9:14 956.
dndrr N.Jrbrour r993. Porchiial dfdsisled redlrique ior
olcndanAeFusspeciesi', oaDtivc. VcloinrryRcmrJ. l.ll : 159-163
IreveridSd, r) R
lhc consc{ariod
Bo.diono, A., Y. Rusi'€trroN arld R.A. Godku. 200i Developdeni olr,
t.odMed
"u, Ani'r
capriic en bryos cu Ltu
diltctonlondniors. lhe7ndlnr M*ring Bi.rech
Rco..n. Ktrnrnlll China.
r.d
Bmckcll e.G.
and C. Oliphadr. 1975. Crpxcluridr
Rcprod.l2:260-274.
.lribbir sneftrrozo! d rtru.
Bio.
Bm.kEtr,B.G.mdK. .Zuelko.l99lAnalyshollier.di'v.lvrdi'irhernyrruproducrion
.lbovii.
dmbryos. Thcriogerolos/ 39: 1l-16.
Bturu.B.W.andT.R zirwic. 1993. Producrio otihccp cnibrjiJ;" u'/a |cnitiz.rion d d
dcvclopmenl oa progenl, lolosiDg siielc rnd hvi'i embrlos r.netcr
Thcrioqcnolosy. 19: 152j l5l7
Chc. L.. P.T. Rus*i ed W.J. Lirsef. 199i. rd.rional dijtlit $':tuci!c sp.cificny
ov. appingrh.tolmno(ic p.ore,, kifrscp3tcd.2. Eur. ofl. niochcfr.20i:237 29.1.
Co*ioni. A.C. I{D. Reirl'br.h, J. SctrNanz V.S.N.
Ij
Fltrj, JL. R.llriquc! rnd A
Bmndclli. 2001. SPrm FuDciior and Prodr(ion ol Bovinc Edbr_vos in Viao Afi.r
Srimp Up lnh DilTer.ni CalciuD ornl Cafcinc Devetopncfi
Shc.p. Gamcr
Rcsearh 16i I59-l?0.
ii
D.
V.. N. Crcze! M. Ahmed-Ali,
lvanino and Y Cognic. t992.
marrarionrnd ldliLi/!ril .l8oa{ooc}rcs. The.iogetrol(gv. 37:t049 1060
Smedr.
.t
rrm
Djuwita r. 200r. Kaji:n Morlologid!r FunEsi Biot.sis O.rir Domb! Scrct.h Kriop.cseryrsi
l)rnern Metodr virrivikrsl. Di$ndsl P.sdsarjam l l'u, Bogoi.
t.en,ncy,lR.l93T.CullureolAfinalCclk.AnranlaLolBasicTcchnique.AhnR.tlss,
1937. 1,
rtru
lcniLiTriior in
nmiia s. L
Reprod. i,eft. Suppl.
1991. Conad.topln Equncncnr lor rhe
tlrn s'bsc!Nni efi brlo'ilc dc!clopnenr
1031 1091.
Co'Llon,
I I99l
l-rbo' .ry P,od!.ri.i.lCr(le
Edrbrtds
tli(iechrlosr. ni Aari.trl1trrxl
^K
lrs rN1ll)(Jstts'\Pt
YANri otill]\TANcK,\NSt(.AR^ / ' r,77F'
r.ANr'\ u
(rleh lJ.lbcn
Sani
iDjbrsrhhin$nlai l,rulD.l..ll strr.diMs..r,tsdirjDr.t..lt. Jayaodi.llls)
RN(IIi,\SAN
liOa)a mcningkrtlan ptuduhivnrs sini mcLtrlui ncmr3|]ln r.tDdlosi trdiist ,l
=btr!.L rllemarilF.dLk! ed)i.drl. i nchk$nIi tDdit'iraptrnNan0unr.riulJ.lr
:.::_trlxn
ltxtuh lanN lel]ih ine0eutrtui-jliu lsknotogi l.(il\r\i ,r rr/, Nnrp.k.
-
-.:: r!i
(trt,kprontr[ri eDibri.t]dali0!Lurgm bLrr2n (di t!dr'btrh)
P. .lirirn inl b€nulurtr trrtrk Drcigdrhri psng:ruh ta r \ratru hns0otusircdrdrp
::.r l.tr raigrn..ri( srf. n,errcr.htri pcns.uh .lalir! hn\omsi terhsdop Itrgkd
':. 15ilosir \api. (r,An lanq DernuiJ-ri b.bc.apr lrfnscttumulL'r k.m|]ak d nrar.dgk.n
s::-: ,, rrfu nengelrakaD nfdiu JiM r9r) )-rtre disrpLcmcnrrsi denSri t r./.
- :,..rrr! r/l,/,o,. (lSI l) l0 rg/ml.s.rum saf 0t; dxn grnrrnisi'r 50 lL!htpdrca\rn
t: ori nrnl) !! anr 11.iir p.di suh! 13.5!c d:hn irktrtixlor tur i9:o
P(nrlurn adrah I Frlode hnrx \xlrL ransf.ft.s .rarn'm rairl l jan. 7jxm. r0
r! l i ro lr
. , J:h...J
.,{o,(t.t.
,".n
'
,n' i
'' lJ't'
.- n
'-.,
i:: nni Ltrrlir! olril urluk s.riip pcrhLnn Dr.ftrduttrn p3rurrDar kurtilr\..ri(
!e-rtif
:._:.i b. rmbxhiyr hDr \!rli' hn\0.nrsi srjd d n.rotr! Ljcolln ra esiir di
-. -ri!L[.ti pcrh.duf ] r! trrrrn (P.1051 x . bc.hrsaj i.rtikurn trn \rltu
:r_ii\ri\i. Tinlhl kciubnlad re trlgiprdr*2knr rn sponrjiljxn yairr srbAar j6 25
1r_ !trf! rctundrh nrdr D(lakuali \.[rr rnLnroodrs ]tjim!irtrseb.rar262j Oosn!rDg
:r. nr.n.rpri hhrf D,di0g ar.n Nlll dr|r]r rcrruli prda hcdDlaj thap scbc trnnya
::!ricrmlind \k!cr. ((lv). Gc.m ml veri.l. I]..itdo\m ((jvrD), Mclrlisc I. frfik
r T.l.,[]!c I l'.i\ctru* oori lc.linili\asi -,rr! LLcnsan n.isguiakan scmcn el]i dari
... rrn! borsrl diri scrrcn roi \i'neirrt \irig LtipfudLtsi .ch BtB Turh Srtrb
:.-rirmbuh Rrli rdi psscn{ric losirrang r..r'cnrli:rsi
:
-.
lcdins-!i diFro ct pad. p.rtrIun
trtrsponasi ,l .r.rh r,rtu lcbt\ar 6t 62 dad lc.rndxh orda p$aLran tan \raLtr,
ir rrnu scl,c$r ll50. Pchilrs. ..sn reftini issi padr bcha|aip.rL.Irn lanuN]kru
:\'nr!i .rirnn s.b.gni !'mhc. oo\ir trrftrlirkrn rd]nrr n.rtrclt n ]rng \rngrt i)rh
: rrlltr
ri.r.rlr.riLro linili:Aioor
r
t)rJ3liN trrLtr hD!Nnari 10-ll
l.bih
iaor
fur
: :rh drrl lior \rlru I m mrfunirlki brh\yr ovrlnrn d,rsih mdnl)Url.j
torcdli
- :ji!$,..r oo!1rnrul p JuL\ enlnlo i !irnr r)r|d disihprt[xn hah$] trDa N.krr
, .:idrsjnchprngrn,hjrjrbl[\difkor\tr1cnri..\nrftk lrrnsltam neDiiaicii tui
oresk
I. PEI{D,/\' II]I,IJAN
l.n\ep |EDbanlud.n p.(cmx[rn adrlrh Dc\{iudkrn k.nd( o.rlh.tan }rD{
-rjtr. .fisicn dxi rxnlgrh Irrg
.ri\i k
dicnikan ol.h
. lc'nxmnun Drenles ai[an
:f,*r scd.
Jrlam
-:.mxlxn
--:-sulan
N]. du {nkrur
|ioduksj dcnlxn
tehuruhai
tcmi ai
Lcmaml]ufDya untuk nc )unbdig re.hadap pcmbrngtrnrn qilavilr.
!*mpdxn [er[ peid3Fhi dxi
!T.n
lcmampuiflr memcnlhi
CcrbiilaD
rrrl
hiduC, pcrbritan ]ineturgir,
tedNrhlhr ek.noni. l\lrnrir scbalai pcngge.r(
Dre',jad
htr |.rc,mk.tr !.brgri irdunri
hioLosis
lcnl
t.nrtr.dgunrd
hirgga ncfjodj tomodirrs
\cdr mdnr)Lr)ai niri ko icrsirliings Khusus unru[ rer k epidiDituxnq
::rpunrlipcr
slrrns.uktrc rrc*rdlhm mctrirgkalkai k.rrhrnan FD3xn. khur'snyr
::r:: l,clnni. nefiir.qliatk klrl1r5 ttsrn' d.u
-:. ig[.rl|n
::!r1 Dila
n ndrnrLn drD krs.j.r,rsrln nrsrr!kar
nrbsirtr\i imo.r. sckxljstrj
hctaLLLi
ncmh€.d]txai ckononrj
usrhr Dremnc.ftia( rju ptuduk\i ccrcft'rlln letrh diti(trkrn bcn]rlri
-:it3 h,,il \cctrru refdekrirf klrilnrril d.ngrr pcrn{ldxn popu]$i rcnrI drr
:.l.i:\diri ILrlnrl]f |tD f.Lkrtri nrodrl5l ne. unir ic mk dcngrn pr.{rn tiscnrj,rlj
l.i
llBl. Ttun\ler
l\nhri(rlr)diii VcdilisrsirJ,!r, (ftV)
UprJl Dmingkrlkrn pFdukiivilrr srni nchLi pctrc..pxn lc[no.gi t enitn.si,]
i.
.
Scbrlri r t.nrr
I rodtrlsi
cmbrio
drhfr nct.l,sarran Tl: dihrrCkaf
mrnipn
trig tbir Drcretrtrrn![rn. ncmbuti] p!tu.irr trtrr,tr
-:-.]!mbaig[rr t.kfk irtriJr]lsi!r ddaf !nb.io. tloni'ig. raNlci k. kjmen d j
-:_:rb.'1, pclcmxlrtr k.rrrr
ir-:n!i-!cnrlik(a;dd.r.9q1).Tc[noLosilentisi,rr/r,mcrucrr]irck!togirnr,lr
-;u[ii.mh.o|trdr ]iqk0 F. budntd lL{rfuljrht.
irr. r,{ fircfttixlr\:.tungLrirD [c!iki ]ris mcLiptrr Fnorif!ii
'
.:1. !in !p.mrbToadrn
krltu, cmtiio.
oo5ir.
ftnile!
Bebenpr |]tnelirirn poduk\l ernhio scc..a
refluk scpcftl sipi
(l
r/,
rcunsoi (r
199,1
dln
z rrlm lchh
ditaporkan padi b.rbrgri
r/., 2001). lterhau niey zt
BocdioDo ?!
,lls9l).do'nba(Bro{n./r/.,l993.drnla*\rndi.rrl.,200t).tambing(pr*srrc.rd,
199,1)
Janpadrkucins.rniinsdancheehh(Bevc.idg.danJabi.u..
In,Jtr[sicnbrtu,
Dr am
d norone
i?rD
o.sir r-rng dilunaltln
di RPll (Runiah Po.org Il.N.n)
dip.rolch n.laluircknLk
rl.
,
'nlrah
Lomplck. Kcddtrlr
dri
nc
b.nlur Lllnrah.tnf) I.thl d
l{flt Dr.ruFtar
di|c6lch dxri
sumber
I 'jjrn
rr!,
rlV
ooslr dari he\aD
Nlenu.u( ihne
sc(chh ncmobigan
naisponasi
rrnuksl eirhrio ,r
lcbih mrhal dai mcmblr'hkxn psrtahn ratrB cbih
ri li.r.n
RPll dcngan Lrbo.nroriLLm
rp.di \vilN
an8
dari hcrqn hidrp lxng
did teAcdia lal.m junnrh han)rk. scdrigltrtr rxns dil](o.h de4r.
r.\rik arrr,, trt ,ir cl.ril
Jtrlrir \r.kn,
dqd bensatdrrisapi ya !
xri di petul.h
(Ol'Ll) d.ngrn
Koch.r././.,20021 Oorit
1999 dxn
msn !an!
Oryr,lnr U/
I993).
.lu'
.r
)'tr.
diCDl.ns adrhh
!1..(200:l)
orrriun
irrrt arrrn
harus dikot!ksi
Peniis(dr xrltt' lsjak rrc\str
nrcntrrun[rn
v]rlilnrs oorir
raDS
rcnnasutrxhap ncn ratrsaf doi r.nilisasirrr
diNrodg
dilunxtaf um'k
rirn, Rcdasartaf
.iparai di rtat !fr!L D,ctrlcuhui lxmr *r[ltr rxnsp.turi .\r.iuD $0i ftrh
\m.ron?ri sanpri lc hbo{rornim FIV )rDS tcrbrik dxn
Drsfda|alkai rtrlko
-mdafgrd oosn )_rd! ri,r!sL mrka nJntr!s tr,.Lrkuk. pcncliliai d.rgrD jLldut
Pcigrrun
Lrhr \txklu T..nspodxsis.icrah
ilpirrog
di
M|t,nqrxn Sccrm r, t ?rr.
F.Rordngrn tcrhrd3n
:
5lr(6 t riOosi.
V, KESTMPULAN DAN SARAN
Bederkan h.sil dri
r
penelirian inimak!dap.r
di6rik bcbcrjpr kesinrputan:
l-an!rvaku tanspotusi menpongrruhj viabitiras
hcngalami pemarangan nri d.n fe.lilisasi,
b.
Pe6enrac pemalaq. n
PcMn rase
oos ir
narang
m en uru
un
n dcngan
datr komperensi oosit unruk
rrro
senatin lrmanya
rlk waktr' ran sporhsi
\yakr u
rrn
sponas i.
da. Bja,nnEsing
4. 7, t0
frasins adalah 56.25. 46.25. 31.75 dan 26 25%
c. P.nenbse @sir
tertijniiisrsi mciurun detrgan senrakin tafranla lvaktu tnnspotusi.
Pe6enraseoosnrcrlcnilissi unlukwakturansponlsi,l,T, t0drn
masing aditah 62.62.
,13 6,1,
44.79 dan
tjjindasing
t2 50%
Bc.dasarkar hasil pcnclirirn dan kesi'npulai drtam penetiri{
inj n:ka
dapd
dikcrukanm bcberapa s..aD kbr8 ibo.ikur.
a. Un(uk mcdap.tkan penenEsD o.sir marang yafg r$baik
harus dikoleksi
osiinyr datafr *ak!u
ktrrang dari 4
*.a
jrm, ufruk msjnperlah.r
!i$ilibsdarkompcrensioostrdnluIfcrlitisasiryrropcrLLkajiar].bihlrnjui
b PerlukrjarlcbihLnjtrlie
pe;kernbancan
.mbri. lr
ada
'/'r,.
p pengrnrh
l.m
wa(to hn
spo das i
1.d.d ap
Ailotro
T.l.L. SlhN.az, N.L fner Md M.l-.l.eibaried,Ruttedec. 1996. AsOdclr oftDlti!1.
aid $agc rhar aflcsr
Itro maru.arioi and dcvclopmcnr ol boline oflcs
',
Therioreidlosy 45:9:14 956.
dndrr N.Jrbrour r993. Porchiial dfdsisled redlrique ior
olcndanAeFusspeciesi', oaDtivc. VcloinrryRcmrJ. l.ll : 159-163
IreveridSd, r) R
lhc consc{ariod
Bo.diono, A., Y. Rusi'€trroN arld R.A. Godku. 200i Developdeni olr,
t.odMed
"u, Ani'r
capriic en bryos cu Ltu
diltctonlondniors. lhe7ndlnr M*ring Bi.rech
Rco..n. Ktrnrnlll China.
r.d
Bmckcll e.G.
and C. Oliphadr. 1975. Crpxcluridr
Rcprod.l2:260-274.
.lribbir sneftrrozo! d rtru.
Bio.
Bm.kEtr,B.G.mdK. .Zuelko.l99lAnalyshollier.di'v.lvrdi'irhernyrruproducrion
.lbovii.
dmbryos. Thcriogerolos/ 39: 1l-16.
Bturu.B.W.andT.R zirwic. 1993. Producrio otihccp cnibrjiJ;" u'/a |cnitiz.rion d d
dcvclopmenl oa progenl, lolosiDg siielc rnd hvi'i embrlos r.netcr
Thcrioqcnolosy. 19: 152j l5l7
Chc. L.. P.T. Rus*i ed W.J. Lirsef. 199i. rd.rional dijtlit $':tuci!c sp.cificny
ov. appingrh.tolmno(ic p.ore,, kifrscp3tcd.2. Eur. ofl. niochcfr.20i:237 29.1.
Co*ioni. A.C. I{D. Reirl'br.h, J. SctrNanz V.S.N.
Ij
Fltrj, JL. R.llriquc! rnd A
Bmndclli. 2001. SPrm FuDciior and Prodr(ion ol Bovinc Edbr_vos in Viao Afi.r
Srimp Up lnh DilTer.ni CalciuD ornl Cafcinc Devetopncfi
Shc.p. Gamcr
Rcsearh 16i I59-l?0.
ii
D.
V.. N. Crcze! M. Ahmed-Ali,
lvanino and Y Cognic. t992.
marrarionrnd ldliLi/!ril .l8oa{ooc}rcs. The.iogetrol(gv. 37:t049 1060
Smedr.
.t
rrm
Djuwita r. 200r. Kaji:n Morlologid!r FunEsi Biot.sis O.rir Domb! Scrct.h Kriop.cseryrsi
l)rnern Metodr virrivikrsl. Di$ndsl P.sdsarjam l l'u, Bogoi.
t.en,ncy,lR.l93T.CullureolAfinalCclk.AnranlaLolBasicTcchnique.AhnR.tlss,
1937. 1,
rtru
lcniLiTriior in
nmiia s. L
Reprod. i,eft. Suppl.
1991. Conad.topln Equncncnr lor rhe
tlrn s'bsc!Nni efi brlo'ilc dc!clopnenr
1031 1091.
Co'Llon,
I I99l
l-rbo' .ry P,od!.ri.i.lCr(le
Edrbrtds
tli(iechrlosr. ni Aari.trl1trrxl