28 considerations of Good Food Production Practice for home industry that
was issued by The Indonesian National Agency of Drug and Food Control 2003 and are designed based on the capacity and capability of the jamu
gendong producers by taking into account their financial status.
3.3.2 Developing Guidelines For a Good Jamu Gendong Production
Practice GJGPP Indonesian National Agency of Drug and Food Control, 2003
Guideline development is based on the observations and laboratory results that was previously conducted. Development was by taking into
considerations of Good Food Production Practice for home industry that was issued by The Indonesian National Agency of Drug and Food Control
2003 and the results of evaluation of jamu gendong production process. Other considerations was based on the capacity and capability of the jamu
gendong producers thats practical and easy to follow by considering their financial status.
3.3.3 Implementation of Good Jamu Gendong Production Practice
GJGPP
Guidelines were implemented on the producers that has participated since the beginning of the study. This was conducted by
visiting the location of the production and perform a socialization and training to the producers regarding the guidelines and carry out a carefull
supervision throughout processing to ensure the applicabillity of the guidelines and identify the Critical Control Points in the implemented
processing using the CCP decision tree.
3.3.4 Product and Facility Microbiological Evaluation Before and After
Implementation BAM, 2010
Product from the production was taken to the laboratory for evaluation of microbiological quality Total Plate Count product,
environmental and bottle, Salmonella sp. and Staphylococcus sp. Analyzes and pH. Product evaluation is conducted before and after implementation to
see the improvements of the product microbiological parameters. Products that was evaluated were beras kencur and temulawak extract. Methods that
was used are as follows:
3.3.4.1 Environmental Air Evaluation
Salustiano et al 2003
The number of airborne microorganisms of jamu gendong processing
areas of the production site was evaluated by culture settling plate
method. Five point in the area were evaluated. The numbers of
aerobic plate count were determined using plate count agar PCA
by opening petri dishes
containing 15-20 ml of culture media and were distributed at the production site and exposed for about
15 to 30 minutes. The Petri dishes were closed and incubated at
48 ± 2 h at 35°C. The
29 colonies were counted using standard counting rules and the results were
expressed as colonyhrm
2
.
3.3.4.2 Microbial Contamination of Jamu Gendong Bottle
Fifty mL of buffer phosphate was rinsed into the investigated bottle. Serial dilutions were prepared to achieve readable numbers of colonies
according to BAM 2010 in the range of 25-250 colonies. These dilutions generally were 10
-1
up to 10
-6
. Ten mL of the investigated sample was aseptically transfered to 90
mL of diluent 10
-1
, then 1 mL from previous dilutions was aseptically dispensed into the next dilutions. One mL of the last three dilutions was
aseptically dispensed onto duplicate Petri dishes using a pipette and sterile tips. Fifteen to twenty mL of plate count agar which was tempered
– heated and kept at 45 ± 1ºC in the liquid state was aseptically poured over
pour-plate method and immediately mixed thoroughly and uniformly by a circular and side-to-side motion of plates on flat level surface. After
solidified, the agar plates were inverted and incubate promptly for 48 ± 2 h at 35°C.
∑ ∑
3.3.4.3 Product Total Plate Count BAM, 2010
Serial dilutions were prepared according to to achieve readable numbers of colonies according to BAM 2010 in the range of 25-250
colonies. These dilutions generally were 10
-1
up to 10
-7
. Total plate count evaluation procedures are as stated in 4.2. The
colonies were counted using standard counting rules and the results were expressed as CFUmL. Counts computed should be in the range of 25-250
colonies.
∑ [
where: N = Number of colonies per ml or g of product
∑ C = Sum of all colonies on all plates counted n1 = Number of plates in first dilution counted
n2 = Number of plates in second dilution counted d = Dilution from which the first counts were obtained
