marmorata n. nebulosa MOLECULAR PHYLOGENY AND GENETIC DIVERSITY OF FRESHWATER EEL GENUS Anguilla IN INDONESIA
44 A. b. pacifica supported by 99 replicatons bootstrap subspecies that
inhabit in eastern of Indonesia water have three nucleotide diagnostic T at 449
th
, T at 466
th
and T at 711
th
and three amino acid diagnostic L, M, L at codon 156
th
, 231
th
and 236
th
respectively with all species. A. celebensis supported by 99 replicatons bootstrap be identified clearly
with five nucleotide diagnostics T at 182
th
, T at 518
th
, T at 582
th
, G at 605
th
and T at 734
th
with all Anguillid species. According to Figure 4, A. celebesensis consist of two clades bootstrap 100. Clade-1 has one nucleotide diagnostic T at 746
th
, supported with 81 replication bootstrap and clade-2 has two substitution
nucleotide diagnostic G at 542
th
and T at 981
th
with all Anguillid species, supported with 99 replication bootstrap. Noticing the topology branch of clade-2,
it divided into two subclade, which supported by significant bootstrap 83 and 99, however we did not find diagnostic nucleotides for the subclade. Genetic distance
within species A. celebesensis also have the large value in comparison to other species, this fact supports high divergence of this species.
A. interioris supported by 99 replicatons bootstrap have two substitution nucleotide diagnostics T at 536
th
and T at 920
th
with all species. In accordance Fig. 7 A. interioris divided into two clades, clades-1 support by 78 bootstrap
have one nucleotide diagnostic G at 873
th
and clade-2 support by 71 bootstrap have 1 nucleotide diagnostic T at 650
th
. There was one specimen Men 4 out of both of clade.
Table 8. Nucleotide diagnostic of each species and clade on the species
nucleotide position nucleotide position nucleotide position nucleotide position nucleotide position nucleotide position nucleotide position C
nt-38 T
nt-182 A
nt-113 T
nt-449 nd
nd G
nt-338 T
nt-536 T
nt-68 T
nt-518 T
nt-305 T
nt-466 T
nt-410 T
nt-920 A
nt-95 T
nt-582 T
nt-482 T
nt-711 T
nt-696 T
nt-101 G
nt-605 G
nt-635 G
nt-986 T
nt-152 T
nt-734 T
nt-644 T
nt-164 T
nt-705 G
nt-392 T
nt-878 T
nt-435 A
nt-465 T
nt-467 A
nt-491 T
nt-686 G
nt-710 T
nt-755 nd
nd T
nt-746 C
nt-215 nd
nd C
nt-20 nd
nd G
nt-893 G
nt-386 G
nt-902 nd
nd G
nt-542 G
nt- 362 nd
nd G
nt-485 nd
nd T
nt-650 T
nt-891 G
nt-620
A. n. nebulosa A. interioris
Nucleotide diagnostic for
Species and subspecies
Clade 1 Clade 2
A. b. bicolor A. borneensis
A. celebesensis A. marmorata