Potensi Leukemia Inhibitory Factor dalam peningkatan kemampuan conditioned medium untuk pengarahan embryonic stem cells mencit menjadi cardiomyocyte
POTENSI LEUKEMIA INHIBITORY FACTOR DALAM
PENINGKATAN KEMAMPUAN CONDITIONED MEDIUM
UNTUK PENGARAHAN EMBRYONIC STEM CELLS
MENCIT MENJADI CARDIOMYOCYTE
DWI AGUSTINA
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
BOGOR
2009
PERNYATAAN MENGENAI TESIS DAN
SUMBER INFORMASI
Dengan ini saya menyatakan bahwa tesis Potensi Leukemia Inhibitory
Factor Dalam Peningkatan Kemampuan Conditioned Medium Untuk Pengarahan
Embryonic Stem Cells Mencit Menjadi Cardiomyocyte adalah karya saya dengan
arahan dari komisi pembimbing dan belum diajukan dalam bentuk apa pun kepada
perguruan tinggi mana pun. Sumber informasi yang berasal atau dikutip dari
karya yang diterbitkan dari penulis lain telah disebutkan dalam teks dan
dicantumkan dalam Daftar Pustaka di bagian akhir tesis ini.
Bogor, September 2009
Dwi Agustina
NRP: B151060061
ABSTRACT
DWI AGUSTINA. The Potency of Leukemia Inhibitory Factor in Increasing the
Ability of Conditioned Medium to Promote Mouse Embryonic Stem Cells into
Cardiomyocyte. Under Direction of ITA DJUWITA and ADI WINARTO.
Embryonic stem cells (ESC) are self-renewing and pluripotent cells
derived from the inner cell mass of blastocyst stage embryo that capable to
differentiate into all type of cells including cardiomyocyte (cardiac muscle cell).
Recently, there are several published methods to increase the number of
cardiomyocyte differentiated from mouse ESC. In the present study, we
examined the effect of conditioned medium (CM) collected from cardiomyocyte
primary cultured of neonatal mouse modified with leukemia inhibitory factor
(LIF), designed as CML, on the differentiation of mouse ESC toward
cardiomyocyte. The media tested were: (1) DMEM, (2) DMEM+CML0, and (3)
DMEM+ CML10, with the ratio of DMEM and CML was 1:1. The three media
were tested on to ESC colonies, each consisting of 8 colonies. Differentiation of
ESC into cardiomyocyte were indicated by: (1) the presence of beating area in
ESC colonies, and (2) the genes expression of Nkx2.5 and -MHC in
cardiomyocyte differentiated from ESC. The results of these research showed
that: (1) ESC colony treated with DMEM+CML10 showed the number of
cardiomyocyte-like cells (indicated by beating area) was higher than the
DMEM+CML0 and DMEM (P
PENINGKATAN KEMAMPUAN CONDITIONED MEDIUM
UNTUK PENGARAHAN EMBRYONIC STEM CELLS
MENCIT MENJADI CARDIOMYOCYTE
DWI AGUSTINA
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
BOGOR
2009
PERNYATAAN MENGENAI TESIS DAN
SUMBER INFORMASI
Dengan ini saya menyatakan bahwa tesis Potensi Leukemia Inhibitory
Factor Dalam Peningkatan Kemampuan Conditioned Medium Untuk Pengarahan
Embryonic Stem Cells Mencit Menjadi Cardiomyocyte adalah karya saya dengan
arahan dari komisi pembimbing dan belum diajukan dalam bentuk apa pun kepada
perguruan tinggi mana pun. Sumber informasi yang berasal atau dikutip dari
karya yang diterbitkan dari penulis lain telah disebutkan dalam teks dan
dicantumkan dalam Daftar Pustaka di bagian akhir tesis ini.
Bogor, September 2009
Dwi Agustina
NRP: B151060061
ABSTRACT
DWI AGUSTINA. The Potency of Leukemia Inhibitory Factor in Increasing the
Ability of Conditioned Medium to Promote Mouse Embryonic Stem Cells into
Cardiomyocyte. Under Direction of ITA DJUWITA and ADI WINARTO.
Embryonic stem cells (ESC) are self-renewing and pluripotent cells
derived from the inner cell mass of blastocyst stage embryo that capable to
differentiate into all type of cells including cardiomyocyte (cardiac muscle cell).
Recently, there are several published methods to increase the number of
cardiomyocyte differentiated from mouse ESC. In the present study, we
examined the effect of conditioned medium (CM) collected from cardiomyocyte
primary cultured of neonatal mouse modified with leukemia inhibitory factor
(LIF), designed as CML, on the differentiation of mouse ESC toward
cardiomyocyte. The media tested were: (1) DMEM, (2) DMEM+CML0, and (3)
DMEM+ CML10, with the ratio of DMEM and CML was 1:1. The three media
were tested on to ESC colonies, each consisting of 8 colonies. Differentiation of
ESC into cardiomyocyte were indicated by: (1) the presence of beating area in
ESC colonies, and (2) the genes expression of Nkx2.5 and -MHC in
cardiomyocyte differentiated from ESC. The results of these research showed
that: (1) ESC colony treated with DMEM+CML10 showed the number of
cardiomyocyte-like cells (indicated by beating area) was higher than the
DMEM+CML0 and DMEM (P