Lampiran 4. Penghitungan Bakteri Vibrio di Tubuh Udang.
Hari Pengukuran 2
4 6
8 10
Infeksi tunggal
V. harveyi 10
7
cfuml
Koloni Hijau KHB
2000 200000
100000 300000
Total Vibrio TVC
3000 21000
~ ~
~ ~
~ 5600000
900000 300000
800000 560000
1300000 ~
~ 6900000
5900000 4600000
Rataan KHB
2000 200000
TVC
12000 600000
680000 1300000
6900000 5366667
KHB
0.00 0.00
0.00 0.00
0.03 3.73
Ko-infeksi IMNV dan
V. harveyi 10
7
cfuml
Koloni Hijau KHB
20000 40000
8000000 50000
70000 1570000 1030000
~ 2820000
~ 10500000
11000000 13400000
Total Vibrio TVC
3000 21000
310000 190000
~ ~
~ 13800000
500000 380000
1870000 1030000 ~
~ ~
13300000 13500000 14600000
Rataan KHB
45000 1300000
2820000 10500000
10800000
TVC 12000
345000 1450000
3000000 13300000
13966667
KHB
0.00 13.04
89.66 94.00
78.95 77.33
Control
Koloni Hijau KHB
Total Vibrio TVC
3000 21000
~ ~
~ ~
~ ~
70000 40000
100000 430000
~ ~
~ 2300000
~ 1200000
Rataan KHB
TVC
12000 55000
265000 3000000
2300000 1200000
KHB
0.00 0.00
0.00 0.00
0.00 0.00
Lampiran 5. Perhitungan Bakteri Vibrio di Air Pemeliharaan.
Hari Pengukuran 2
4 6
8 10
Infeksi tunggal
V. harveyi 10
7
cfuml
Koloni Hijau KHB
~ ~
~ ~
~ ~
1240000 980000
~ 2470000 100000
680000 250000
180000 360000
Total Vibrio
TVC ~
~ ~
~ ~
~ 50000
110000 1650000
~ ~
~ ~
5300000 ~
3100000 ~
6300000
Rataan KHB
1110000 2470000
100000 465000
270000
TVC
80000 1650000
3000000 5300000
3100000 6300000
KHB
67.27 82.33
1.89 15
4.29
Ko-infeksi IMNV dan
V. harveyi 10
7
cfuml
Koloni Hijau KHB
~ ~
~ ~
~ ~
2840000 1820000 ~ ~
~ 10100000
~ 6500000
~ 8700000
Total Vibrio
TVC ~
~ ~
~ ~
~ ~
~ 50000
110000 ~
~ ~
3300000 ~
17000000 ~
14500000 ~
16700000
Rataan KHB
2330000 3000000
10100000 6500000
8700000
TVC
80000 3000000
3300000 17000000
14500000 16700000
KHB
77.67 90.91
59.41 44.83
52.10
Control
Koloni Hijau KHB
Total Vibrio
TVC ~
~ ~
~ ~
~ 50000
110000 ~
~ ~
4200000 ~
7300000 2300000
~ 9800000
Rataan KHB
TVC
80000 3000000
4200000 7300000
2300000 9800000
KHB
Lampiran 6. Hasil Analisis Uji T MINITAB 16 Jumlah Bakteri Vibrio Hijau Berpendar dan Total Vibrio pada
Perlakuan Infeksi Tunggal
V. harveyi 10
7
cfuml dan Ko-infeksi IMNV dengan
V. harveyi 10
7
cfuml pengambilan sampel di 10
dpi.
1. Analisis uji T MINITAB 16 jumlah bakteri Vibrio hijau berpendar.
Two-sample T for Ko-infeksi vs Infeksi Tunggal N Mean StDev SE Mean
Ko-infeksi 3 10800000 2705550 1562050 Infeksi Tunggal 3 200000 100000 57735
Difference = mu Ko-infeksi - mu Infeksi Tunggal Estimate for difference: 10600000
95 CI for difference: 3874452, 17325548 T-Test of difference = 0 vs not =: T-Value = 6.78 P-Value = 0.021
DF = 2
2. Analisis uji T MINITAB 16 jumlah total Vibrio.
Two-sample T for Ko-infeksi vs Infeksi Tunggal N Mean StDev SE Mean
Ko-infeksi 3 13966667 568624 328295 Infeksi Tunggal 3 5366667 680686 392994
Difference = mu Ko-infeksi - mu Infeksi Tunggal Estimate for difference: 8600000
99 CI for difference: 5609008, 11590992 T-Test of difference = 0 vs not =: T-Value = 16.79 P-Value = 0.000
DF = 3
Lampiran 7. Bobot Udang Uji gram pada Percobaan 1.
1 2
3 4
5 6
7 8
1
2.2 3.2 2.4 2.4 2.7 2.6 2.9 3.1
2
2.4 2.7 2 2.3 2.8 3.1 3.3 2.3
3
2.9 3 3.3 2.4 2.5 2.9 2.6 2.8
4
3 2.1 2.8 2.3 3.1 3.2 2.4 2.3
5
2.7 2.4 3.4 2.3 3.2 3.4 2.7 2.4
6
3 3.2 2.9 2.1 2.3 2.7 2.4 2.6
7
2.1 2.1 2.8 2.5 2.3 2.6 2.1 2.9
8
2.5 2.6 2.6 2.9 3.3 2.6 3.1 3.3
9
2.9 2.4 2.8 2.2 3.5 3.5 2.6 3.2
10
3.1 2.5 2.3 2.9 2.3 2.1 2.5 3.2
11
3.6 2.5 2.3 2.8 2.4 2.1 3.6 2.6
12
2.7 2.1 2.9 2.8 2.8 3.6 3.2 2.5
13
2.4 2.3 2.2 3.4 2.5 3 3.4 2.8
14
2.7 2.7 2.9 2.1 2.9 2.3 2.6 2.9
15
2.4 2.9 2.5 2.8 3.1 2.3 2.3 2.8
16
2.1 2.8 2.3 2.5 2.9 2.9 3.1 2.1
17
2.3 2.2 3.4 2.8 2.5 2.6 3.6 2.9
18
2.3 3.5 3.5 3 3.2 2.5 2.9 3.2
19
2.2 2.8 2.3 3.3 2.4 2.4 2.9 2.6
20
2.4 3.3 2.8 2.4 3.1 2.4 2.7 2.9
21
2.3 2.6 2.5 2.8 2.3 3.4 3.1 2.5
22
2.6 2.3 2.6 3.2 2.4 3 2.5 2.4
23
3.3 2.4 2.6 2.1 2.9 2.2 2.4 2.3
24
3.1 2.8 2.6 2.9 2.6 2.4 2.8 3.3
W total = 519.9 gram Jumlah = 192
udang
Rataan = 2.708 gramudang STDEV = 0.395 gram
Lampiran 8. Bobot Udang Uji gram pada Percobaan 2.
1 2
3 4
5 6
7 8
9 10
11 12
1
3.4 2.9 2.6 2.9 2.5 2.5 3.2 2.9 3 3.4 2.7 3.4
2
2.8 3.1 3.4 2.8 3 2.4 3.5 2.9 2.9 2.5 3.2 3.2
3
3 2.7 3.4 2.6 3 2.9 2.6 2.6 2.7 2.6 3.2 2.9
4
3.5 2.6 3.2 3.4 3.1 3 3.2 2.8 2.7 2.8 2.9 2.6
5
2.5 2.5 2.7 3.2 3.2 2.9 2.8 3.5 3.2 2.9 2.8 3
6
2.8 2.8 3.5 2.6 2.5 2.8 2.5 3.3 3.2 3
3 2.8
7
2.6 2.9 2.8 2.5 2.3 3.4 3.2 2.8 3 2.6 2.6 2.8
8
2.7 2.5 2.4 3.1 2.9 2.5 2.6 2.6 2.6 2.6 2.7 3.5
9
3.4 2.9 2.6 2.5 3 3.4 3.2 2.9 2.6 2.8 2.8
3
10
3.3 2.7 2.4 3.1 2.5 3.3 3 2.7 2.5 3.4 2.9 3.5
11
3.4 2.6 2.4 2.9 2.5 3.3 2.5 3.6 3.1 2.9 2.8 2.8
12
2.4 3 2.8 3.1 3.2 2.9 3.2 2.6
3 3.1 2.5 3.3
13
3.2 2.5 3.2 2.9 2.8 3 2.7 2.9 2.6 3.2 3.3 3.4
14
3 3.5 3.1 2.6 2.4 2.8 3.2 3.1 2.8 2.9 2.6 2.7
15
2.8 3 2.7 2.9 2.6 3.4 2.8 2.6 3.3 3.4 3.4 2.9
W total
= 523.8 gram
Jumlah
= 180 udang
Rataan
= 2.91 gramudang
STDEV = 0.312
gram
ABSTRACT
ANWAR HASAN. Co-infection of Infectious Myonecrosis Virus IMNV and Vibrio harveyi
in Pacific White Shrimp Litopenaeus vannamei. Under direction of SUKENDA and WIDANARNI.
L. vannamei production in Indonesia was growing intensively. In 2006,
IMN infectious myonecrosis disease was confirmed spread to Indonesia shrimp farm and declined shrimp production. The study was conducted to investigate
effect of dose of V. harveyi on co-infection with IMNV in L. vannamei as well as development of viral infection. Shrimps juvenile were oral infected with IMNV
infected shrimps 10 feeding rate during 3 days and co-infected with 10
6
, 10
7
and 10
8
cfuml V. harveyi. Mortality rate was 0 in control and single infection of V. harveyi except 10
8
cfuml treatment. Mortality pattern demonstrated on co- infection was faster and higher than single IMNV infection in 14 days
observation. The density of green colony Vibrio in hepatopancreas of co-infected shrimps collected in 2, 4, 6, 8 and 10 days post infection were higher than V.
harveyi
single infected significantly in 10 days post infection. There were no difference of IMN disease development between co-infection and IMNV single
infection. It was confirmed by visual gross sign appeared, tissue and lymphoid organ histophatology, organ abnormality, and PCR test. In conclusion, IMN
disease caused higher and faster mortality on co-infection with V. harveyi, but not affect to IMN disease development.
Keywords: co-infection, L. vannamei, V. harveyi, IMNV, mortality, gross sign
I. PENDAHULUAN
1.1 Latar Belakang
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