Penggunaan Probiotik, Prebiotik dan Kombinasinya untuk Meningkatkan Respon Imun dan Resistensi Ikan Nila Oreochromis Niloticus terhadap infeksi Streptococcus agalactiae
Penggunaan Probiotik, Prebiotik dan Kombinasinya
untuk Meningkatkan Respon Imun dan Resistensi
Ikan Nila Oreochromis Niloticus terhadap infeksi Streptococcus agalactiae
Application of Probiotic, Prebiotic and Their Combinations
for Increasing Immune Response and Resistance of Nile Tilapia
Oreochromis Niloticus against Streptococcus agalactiae Infection
WIDANARNI, ACHMAD FAROUQ DAN MUNTI YUHANA
ABSTRACT
Tilapia have been cultured to meet increasing market demand on fish. Some efforts have been done to increase
tilapia production. However, some parasitical and bacterial diseases often caused mass mortality in fish culture. One of
the bacterial disease that recently attacked tilapia is streptococcosis caused by Streptococcus agalactiae. In this study,
fish feed supplemented with probiotic, prebiotic and synbiotic were applied to enhance the immune response and fish
resistance against Streptococcus agalactiae infection. Treatments used in this study, were as follows : feed containing
1% (v/v) of probiotic, feed containing 2% ( v/v) of prebiotic and feed containing mixed of 1% ( v/v) of probiotic + 2% (v/v)
of prebiotic (defined as synbiotic). In controls (positive and negative controls), fish were fed by fish feed without
containing neither probiotic and prebiotic. In this study, we used all male (monosex) red tilapia with the average of
body weight of ± 13 gram. Fish were fed by supplemented feed within the first 30 days before challenge test by
injection of 109 CFU/ml of Streptococcus agalactiae. The results demonstrated that the survival rate of the fish
challenged by Streptococcus agalactiae in control group (+) was lowest (50%) than others. The survival rate of the fish
fed by supplemented feed containing probiotic, prebiotic and synbiotic were 76%, 76% and 80%, respectively. These
results were statistically different compared to that of control (50%) (P
untuk Meningkatkan Respon Imun dan Resistensi
Ikan Nila Oreochromis Niloticus terhadap infeksi Streptococcus agalactiae
Application of Probiotic, Prebiotic and Their Combinations
for Increasing Immune Response and Resistance of Nile Tilapia
Oreochromis Niloticus against Streptococcus agalactiae Infection
WIDANARNI, ACHMAD FAROUQ DAN MUNTI YUHANA
ABSTRACT
Tilapia have been cultured to meet increasing market demand on fish. Some efforts have been done to increase
tilapia production. However, some parasitical and bacterial diseases often caused mass mortality in fish culture. One of
the bacterial disease that recently attacked tilapia is streptococcosis caused by Streptococcus agalactiae. In this study,
fish feed supplemented with probiotic, prebiotic and synbiotic were applied to enhance the immune response and fish
resistance against Streptococcus agalactiae infection. Treatments used in this study, were as follows : feed containing
1% (v/v) of probiotic, feed containing 2% ( v/v) of prebiotic and feed containing mixed of 1% ( v/v) of probiotic + 2% (v/v)
of prebiotic (defined as synbiotic). In controls (positive and negative controls), fish were fed by fish feed without
containing neither probiotic and prebiotic. In this study, we used all male (monosex) red tilapia with the average of
body weight of ± 13 gram. Fish were fed by supplemented feed within the first 30 days before challenge test by
injection of 109 CFU/ml of Streptococcus agalactiae. The results demonstrated that the survival rate of the fish
challenged by Streptococcus agalactiae in control group (+) was lowest (50%) than others. The survival rate of the fish
fed by supplemented feed containing probiotic, prebiotic and synbiotic were 76%, 76% and 80%, respectively. These
results were statistically different compared to that of control (50%) (P