Slide Presentasi Fermentasi Asam Cuka
Fermentation Proccess of Vinegar
The Usage of Vinegar
Bottled
Dressings &
Sauces
Pickles
Mustard
Other Processed
Foods
Tomato Products
Other
(Crisco Company, 2005)
Balsamic
Distilled
Malt
Wine
Champagne
Sherry
Formation of vinegar
Acetic acid bacteria well know for their ability
to spoil wines because they can produce
large amounts of acetic acid from ethanol.
(Joyeux et al, 1984)
Acetic acid bacteria
Acetobacteriaceae
Gluconobacter
Characteristic of Acetic Acid Bacteria
“
▸Gram-negative
▸Ellipsoidal to rod-shaped cells
▸Aerobic
(Bergey’s Manual of Systematic Bacteriology)
▸Acid tolerant
▸Utilize ethanol as thei energy source
▸Non pathogenic
(Poblet et al, 2000)
Chemical reaction and Formulation
(Kehrer, 1921)
ORLEANS
Method
GENERATOR
Method
O
R
L
E
A
N
S
Drill a hole
Adding fresh vinegar
Bacteria settle into the liquid
Formation of GSL
Fermentation
Solution Volume Setup
Process Timing
Temperature setup
1 Gallons = 30k-35k Btu
G
E
N
E
R
A
T
O
R
Generator Mash Preparation
GM 1 mash [16000 gallon (72738L)]
GM 2 mash [5 gallon (22.731L)]
13500 gallon (61372L) process water
4.22 gallon (19.18L) distill water
150 gallon (682L) 10% filter vinegar
0.047 gallon (0.214L) 10% filter vinegar
2144 gallon (9747L) 190 proof alcohol
0.67 gallon (3.05L) 190 proof alcohol
80 lb (36.3kg) *nutrient
0.064 lb (0.029kg) *nutrient
How about filtration?
MASH
FILTRATION
PASTEURIZATION
Vinegar Qualities
OTHER GASES
Citric acid
Tartaric acid
Malic acid
Lactic acid
Acetoin
Ethyl acetate
Btyrocaltone
Meso-2,3 Butanediol
2-Phenylethanol
Etc
Quality
Control
Alcohol Measurement
pH and Titratable Acidity
10 ml vinegar solution
Adding 1 N NaOH
Adding dry phenolphthalein
(0,002 gram)
Result
PACKAGING
MAPS
$200
£1.59
THANKS!
The Usage of Vinegar
Bottled
Dressings &
Sauces
Pickles
Mustard
Other Processed
Foods
Tomato Products
Other
(Crisco Company, 2005)
Balsamic
Distilled
Malt
Wine
Champagne
Sherry
Formation of vinegar
Acetic acid bacteria well know for their ability
to spoil wines because they can produce
large amounts of acetic acid from ethanol.
(Joyeux et al, 1984)
Acetic acid bacteria
Acetobacteriaceae
Gluconobacter
Characteristic of Acetic Acid Bacteria
“
▸Gram-negative
▸Ellipsoidal to rod-shaped cells
▸Aerobic
(Bergey’s Manual of Systematic Bacteriology)
▸Acid tolerant
▸Utilize ethanol as thei energy source
▸Non pathogenic
(Poblet et al, 2000)
Chemical reaction and Formulation
(Kehrer, 1921)
ORLEANS
Method
GENERATOR
Method
O
R
L
E
A
N
S
Drill a hole
Adding fresh vinegar
Bacteria settle into the liquid
Formation of GSL
Fermentation
Solution Volume Setup
Process Timing
Temperature setup
1 Gallons = 30k-35k Btu
G
E
N
E
R
A
T
O
R
Generator Mash Preparation
GM 1 mash [16000 gallon (72738L)]
GM 2 mash [5 gallon (22.731L)]
13500 gallon (61372L) process water
4.22 gallon (19.18L) distill water
150 gallon (682L) 10% filter vinegar
0.047 gallon (0.214L) 10% filter vinegar
2144 gallon (9747L) 190 proof alcohol
0.67 gallon (3.05L) 190 proof alcohol
80 lb (36.3kg) *nutrient
0.064 lb (0.029kg) *nutrient
How about filtration?
MASH
FILTRATION
PASTEURIZATION
Vinegar Qualities
OTHER GASES
Citric acid
Tartaric acid
Malic acid
Lactic acid
Acetoin
Ethyl acetate
Btyrocaltone
Meso-2,3 Butanediol
2-Phenylethanol
Etc
Quality
Control
Alcohol Measurement
pH and Titratable Acidity
10 ml vinegar solution
Adding 1 N NaOH
Adding dry phenolphthalein
(0,002 gram)
Result
PACKAGING
MAPS
$200
£1.59
THANKS!