KESIMPULAN DAN SARAN

A. Kesimpulan

1. Kerapatan elektron ikatan rangkap C-C MIRA-1 dapat menjadi asimetris dengan adanya pengaruh induksi dari gugus – gugus asam amino dan rantai samping MIRA-1.

2. Ikatan rangkap MIRA-1 dapat terpolarisasi sebagai pengaruh dari lekukan mutasi. Atom O karbonil dari ikatan peptida yang dekat dengan atom O karbonil bagian maleimida dapat menginduksi atom O tersebut untuk memberikan tarikan elektron yang lebih besar sehingga ikatan rangkap C-C kerapatan elektronnya semakin rendah dan dapat terpolarisasi. Polarisasi memberikan kemudahan serangan gugus tiol yang mengindikasi kebolehjadian yang tinggi pada pembentukan adduct tiol.

3. Bagian alifatik MIRA-1 mempunyai gugus karbonil yang memudahkan untuk mendapat induksi dari asam amino yang menjadikan kerapatan elektron ikatan rangkap C-C menjadi rendah dan mempunyai tingkat polarisasi tinggi. Bagian alifatik bersifat fleksibel dapat bergerak pada posisi tertentu dan menyebabkan ikatan rangkap C-C mempunyai kerapatan elektron yang berbeda antar atom C-nya.

B. Saran

Perlunya dilakukan studi interaksi dengan memperbanyak variasi konformasi kompleks p53 termutasi Y220C dengan MIRA-1.

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Bergman, A.R. Fersht, P. Hainaut, K.G. Wiman, and V.J.N. Bykov. 2009. PRIMA-1 Reactivates Mutant p53 by Covalent Binding to the Core Domain. Cancer Cell. 15: 376 –388.

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Lampiran 1. Perhitungan nilai ∆occupancy ikatan rangkap C-C MIRA-1

Nilai occupancy ikatan rangkap C-C

KESIMPULAN DAN SARAN