In Vitro Degradation and Gas Production of Tropical Browse Plants in Different Status

IN VITRO DEGRADATION AND GAS PRODUCTION
OF TROPICAL BROWSE PLANTS
IN DIFFERENT STATUS

Astuti, D.A. 1, A.S.H. Baba 2, N.A. Meta 1 and A. Fitri 1
1 Faculty

of Animal Sciences, Kampus IPB, Darmaga Bogor 16680 Indonesia.
2 Faculty of Science, Universiti Malaya,50603 Kuala Lumpur, Malaysia,

Introduction
¾

Tropical
p
browse may
y constitute an important
p
fodder for
ruminant semisemi-intensive farming

¾

Problem with tropical browse plants is high content of
secondary compound (tannins and saponin) which
reduce the nutritive value (Fall et al.,
al., 1998).

¾

Limited information about tropical browse plants were
fermented in different status, such a single ingredient,
mix with grass or in a complete ration for small ruminant.

Objectives of the research
¾ to

evaluate in vitro dry matter degradation and
gas p
g
production of tropical

p
browse p
plants :
as a single forage
mix with native grass
and in the complete ration

Materials and Methods
¾ Five

tropical browse plants (TBP):
Leucaena leucochepala,
Moringa oleifera
oleifera,
Calliandra calothyrsus,
Gli i idi sepium.,
Gliricidia
i
Artocarpus heterophyllus
¾ Mix grass : TBP = 70 : 30

¾ Mix grass : TBP : by product = 70 : 20 : 10

M i
Moringa
oleifera
l if

Artocarpus heterophyllus

Gliricidia sepium

Callyandra calothyrus

Leucaena
leucocephala

Two fistulated sheep as donor rumen fluid

In vitro DMD (Baba
(Baba et al., 2002)

and
d gas production
d ti
rate
t (Close
((Cl
Cl
Close
and
d Menke,
M k 1996)

Result and Discussion

D e g ra d a tio n   D M   (% )

  In vitro dry matter deg radation with rumen s heep 
(as  a forag e)
50
40

30
20
10
0
Mix G ras s

M. Oleifera

C .callothyrs us

L.
leucocephala

G . s epium

D e g ra d a ti o n   D M   (% )

In vitro dry matter deg radation with rumen s heep
(Mix with Gras s ) )
(Mix with Gras

60
50
40
30
20
10
0
MG +L.
leuchocephala

MG  + M.
oleifera

MG  + C .
callothyrs us

MG  + G .
s epium

MG +A.

heterophyllus

M
G

M
G

on

.  h

h.

..
et
er
. ..

uc

t..
.

i..
.

al
lo

ep

.  c

.  s

.  l
e

+A

+L

+C

+G

nt
ra
te

le
ife
.. .

ce

+M
.  o

+C

M
G

M
G

M
G

M
G

De
eg radation  DM (% )

In vitro dry matter deg radation  with rumen s heep
 (in R ation) 

70
60
50
40
30
20
10
0

Total Gas Production with Rumen Sheep
(as a single forage)
Leucena
leucocephala
Calliandra
C ll th
Callothyrsus
Kelor

T o tta l G a s (m
m l)

200,00
150,00
100,00

Gliricidia Sp

50 00
50,00

grass field

0,00
1

2

3

4

5

Time of Incubation (Hour)

6

Artocarpus
Heterophyllus

Total Gas Production in Ruminal sheep
(Mix with Grass)
T o t a l G a s ( m l)

150,00
GF+Leucena L

100 00
100,00
50,00
,

GF + Calliandra
C
GF + kelor
kl

0,00

GF + Gliricidia Sp

0

2

4

8

12

Time of Incubation (Hour)

24

G

T o t a l G a s ( m l)

Total Gas Production in Ruminal sheep
(in Ration)

GF+Leucena
l+concentrate

150,00

GF+Calliandra
C+Concentrate

100,00

GF+kelor+Concentrate

50,00

RL+Gliricidia
Sp+Concentrate

0,00
0

2

4

8

12

Time of Incubation (Hour)

24

GF+AH+concentrate
GF+Concentrate

Conclusion
¾

The highest DMD was found in M oleifera as
ration, while the highest gas production was M
oleifera as single forage

¾

Moringa
g leaves better be used as p
part of the
ration for ruminants.

ACKNOWLEGEMENT
¾ Thanks

for International Research
Collaboration Grant, Directorate General
Higher Education Republic of Indonesia

Thank you

Further result
¾ In

vivo experiment :

Sh
Sheep
ffed
d with
ith M.oleifera
M.
M oleifera
l if
i th
in
the ration
ti h
has
good immune response with higher
l
lymphocyte,
h
t nethrophyl
th h l and
d IIgG
G compared
d
to other forages