FIGURE 2 biomembra
laser energi spectrum is
for the com
Figure chitosan b
329 nm fo 160 mJ. C
of 120 mJ that the
increasing of electron
excited he The spectr
signal as l continuum
these resu analyzed
optimal co
Figure Ag I at
with laser of depth fr
biomembr emission w
Ag has be the higher
This mea increase, t
process tak
For fu deviation
measuring adjacent a
standard d 2 for las
mJ.
2. LIBS spectra
ane Sample-5 i ies 80, 120, and
s presented kep mparison.
2 shows LIB biomembrane
or varies of lase Control Sampl
J was also kept intensity of A
g laser energy. ns from the ato
ence more em rum also shows
laser energy in m emission of
ults, further ex on laser energ
ondition for the 3 shows the in
wavelength o r energies 80, 1
from the surfac rane Sample-5
was observed een evenly dist
r laser energy ans that the i
the peak becom ke place.
urther examina of the LIBS
g the intensity areas on the
deviation in th ser energy 80 a
a of the pressur in the range 327
d 160 mJ. Contr pt at laser energ
BS spectra of Sample-5 in
er energies 80 m le-1 spectrum
t for the comp Ag I peak
This describe oms and more
mission of spec s increasing in
ncreases, whic the plasma fo
xperiments we gy of 120 mJ
e chitosan biom ntensity of atom
of 328 nm obt 120, and 160 m
ce, of the press 5. The graph
for all depths tributed within
y, 160 mJ sho intensity of t
mes broad and ation of Fig.
intensity was from three ide
sample surfac he intensities o
and 120 mJ, an
rized chitosan 7 - 329 nm for
rol Sample-1 gy of 120 mJ
the pressurize the range 327
mJ, 120 mJ, an m at laser energ
arison. It show increases wit
es more numbe ions have bee
ctrum obtaine the backgroun
ch is due to th orm [14]. From
ere focused an J and stated a
membrane. mic emission o
tained by LIB mJ as a functio
surized chitosa shows that th
s indicating th n the sample. A
ows fluctuation the backgroun
d self adsorptio 3, the standar
s calculated b entical series o
ce. The overa of Ag I was
nd ~ 5 for 16 ed
- nd
gy ws
th er
en d.
nd he
m nd
as of
BS on
an he
hat At
n. nd
on rd
by of
all ~
60
FIGUR wavele
80, 120 for the
To chitosa
treatm 4. For
~ 20 μ Ag I
μm s inclusi
perform adsorp
treated is hea
increas was in
second after h
before Samp
intensi method
The membr
pressu chemic
produc result,
the las Ag I
other a
20 40
60 80
100 120
140 160
180 200
Intensity a.u.
RE 3. Intensity
ngth of 328 nm 0, and 160 mJ as
pressurized chit
study about th an biomembr
ent are introdu natural Samp
μm by laser e obtained was
second shot ion process oc
mance of t ption by heati
d membrane w ated to 35°C
sed to ~ 8.75 a nfluenced by t
d shot was also heating method
dripping it ple-4, gave int
ity of Sampl d was the high
ese results des rane properly w
ure method. cally and phy
ces strong cova providing a s
ser shot and pr at wavelength
adsorption met
20 40 60 8
y of atomic em obtained by LIB
s a variation of d tosan biomembra
he adsorption p rane, four m
uced. The resul ple-2 after first
energy of 120 ~ 6.67 a.u, wh
was null, i ccurred. To im
the adsorptio ing method w
with the same p Sample-3 an
a.u. This indica the temperatur
o zero. Further d was used. He
with 1000 tensity as low
le-5 adsorpti hest at ~ 21.5 a
scribe Ag liqui when pressuriz
This causes ysically with t
alent bonds in t strong rebound
roduced plasma h of 328 nm is h
hods.
80 100 120 140 16 Depth µm
Samp Samp
Samp
mission of Ag BS with laser en
depth from the s ane Sample-5.
process of Ag methods mem
lts are shown in t shot shot-1,
mJ, the intens hile at a depth
indicating tha mprove the dete
on processes was introduced
process of Sam nd the intensi
ates that the pr re. Result from
rmore an adsor ating the mem
ppm Ag sol w as, ~ 5.5 a.u
ion with pre a.u.
id penetrate int zed adsorption
Ag interact the membrane
the membrane d momentum d
a emission inte high compared
0 180 200 220
ple-5, 80mJ ple-5, 120mJ
ple-5, 160mJ
I at nergies
urface
in the mbrane
n Fig. depth
ity of of 40
at no ection
, an d, the
mple-2 ity is
rocess m the
rption mbrane
lution u. The
essure to the
n with both
e and . As a
during ensity
d with
34
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FIGURE 4 328 nm for
The ad
biomembr ions which
with N in only a pai
bond is ca ion is an
donor. Th sufficiently
relatively results of
carried ou relatively
Sample-2 produced
caused a membrane
N that acte a low inten
The p
breakdown of Ag ele
biomembr was analy
waveleng process re
the membr laser ener
process oc without in
treatment a
5 10
15 20
25
S 2
Intensity a.u.
4. Intensity of em
