Sel Penghasil Lisozim Terdeteksi Pada Kelenjar Ludah Sapi dengan Teknik Imunohistokimia
Sel Penghasil Lisozim Terdeteksi Pada Kelenjar Ludah Sapi
dengan Teknik Imunohistokimia
(LYSOZYME PRODUCING CELLS ARE DETECTED TN T H E ROVIATE
SALIVARY GLANDS BY JMMUNOHTSTOCHEMTC'AIA TEC:HL%'IQIJEI
l D t l p ; ~ r t t ~ n ~Anntomr,
vn
Fisioiogi dnn Fal+makologi, Fakultas Kcdokteran H e w a n ,
I n s t l t u t Pcrtaninn Bogor, J1. Agatis Wing 8.T,t. 2, K n m p u s TPR Ilnrmngn Bogor 16680.
TeIp. 025 1-626064,
E-mail: iik-gu~.isG,yahoo.corn
ABSTRAK
1, .I ~. .O ' L I l~~I c l r s i ~1);tktcriolitik
it
dcng;~ncarhamt:r.usak ikat;in glikosid;~p;ld:i clirlcling 11:-ikt,eriwhingga
pt:lacnk;111scl peng;~hsillisozinl pads kclerljijr ludiih sapi pcrlu di1;jkukan. na1;trn penelit,ian ini, sel
pe~lgh;-lsillizozirll d i p e r i k s ; ~~ n r n g g u n a k a nteknik pewarhna;ln histolog'i rian irnunohistokimia. H;~sil
p ~ n r l i t i a nm r n u n j u k k a n biihwa keienjar parotis s:ipi be~nsifi~t
serous rnurrii. Kctlenj;~rsul)m;lntlil,~~l;~ris
bagian nsinarnyn terdiri atas sel-sel serous dnn sel-sel mukous. Sel-sel :i:;i~lnr.irlukr,us tt?rlihat lellih
domin;in ,jik;i ciil~andingkandengan sel-sel asinar serous. Sel-sel asinar kelenjnr pa~qc~tis
s:ipi bt'rr;lksi
positifd(5ngnn ii-ltcnsit;is st!dang (++i
tcn-hadap pewarnaan imunohistokirnia. Sel-sel epitel duktus ktllthrGnr
hcrcnksi scdnng ( + + smnpai dcngnn k u a t ( + + + I , Pnla sebaran lisozinl yang terdeteksi pada pent:!ili;in ini
n ~ r n u ~ ? j u k k ukcmiripan
n
dcngan hcwan mamalia pada umumnya. Sel-sel asinnr serous pad;] ke1rnj:ir
submandibularis rllcmbcrikan rcaksi positif dcngan ill tcnsistas rcndah (+) san-lpai dengan sedang ( + + )
dnn sel-scl epitel d u k t u s kclcnjnr dcngan intcnsitas kuat (+++ ). 1,isoxim ditemukan pada bagian s ~ l - s e l
asirlnr serous dnn sel-sel epitcl duktris kclcrljar parolis dnn submandibularis.
Kata kunci: irnunohistokimia, lisozim, kclanjar lurinl1
I,y*o/.irnrl
. ..
is ~ i i ~ ) i ~of
l ~i ~l tl d! u c i n pdirect bacteriolytic action by hydrolyzing the glycosidc bonds found
in b;lcterial cell wall. A study is thrr.~for.ecorlductetl to detect the lysozymc-producing cells in thc bovine
s:iliv:~rygl:ln(ls. In thi4 study, tissue prepnr.:~tionwas rxaminerl using histolofi-lcaland imn~unol~istochcmicnl
staining. 'Yhr! results showed that bvvirle parotid glands were purely serous acinar*cclls. The s u b m ; ~ n d i h u l n ~
t;rl;111tl,however, contained both rllucous and st:rous :ir:in:ir cells, but the mucous ~ c i n acr d l s were found
to hc I I I O ~ Cdominant t h a n the serous ncinnr cells. Immunrihistochemica1 staining on parotid gland using
antik~otly;ig;linst lyzozirne gave a moderate positive: rt?ilct.ion(++-Ito the acinnr cells and n strong positive
1 +++ 1 to the cpithcliill cclls of the ducts. The distr.ibution ~):~ttctrn
of lyst~zylneproducing crlls urns
~+cnction
similar to tllose of otlzcr mammalian species. In the submnndiliuli~i-ygl;intl, ;I weak i+)t o mediurn ( + + )
positive reaction was drtcctrd in the :tcinnr serous cclls, hilt n .strr>ng11,rwit i v ~
react,ion I +++) ivns dtltclvteri
i n the cpithclinl cclls of t l i r l tlucts. I A y s o Z ~ m
was
c ibun(l 111 t l i r . stht.r,rl:: :~r:in;-11.
~ ~ 1i-tncl
1 s rpithc1i;il rt.115 of the
r l t l r t s in 110th parotid :inti sul)rn:lnriil~ul;l~.y
gl;~ncls.
K c y Word: I ~ n m u n o l ~ i s t o c h c : ~ ~I.yso;lym~,
~ i c i ~ l , S:3li1,;1r.ygliind;:
?Turna1 Veteriner, Mare t 2007
10
dengan Teknik Imunohistokimia
(LYSOZYME PRODUCING CELLS ARE DETECTED TN T H E ROVIATE
SALIVARY GLANDS BY JMMUNOHTSTOCHEMTC'AIA TEC:HL%'IQIJEI
l D t l p ; ~ r t t ~ n ~Anntomr,
vn
Fisioiogi dnn Fal+makologi, Fakultas Kcdokteran H e w a n ,
I n s t l t u t Pcrtaninn Bogor, J1. Agatis Wing 8.T,t. 2, K n m p u s TPR Ilnrmngn Bogor 16680.
TeIp. 025 1-626064,
E-mail: iik-gu~.isG,yahoo.corn
ABSTRAK
1, .I ~. .O ' L I l~~I c l r s i ~1);tktcriolitik
it
dcng;~ncarhamt:r.usak ikat;in glikosid;~p;ld:i clirlcling 11:-ikt,eriwhingga
pt:lacnk;111scl peng;~hsillisozinl pads kclerljijr ludiih sapi pcrlu di1;jkukan. na1;trn penelit,ian ini, sel
pe~lgh;-lsillizozirll d i p e r i k s ; ~~ n r n g g u n a k a nteknik pewarhna;ln histolog'i rian irnunohistokimia. H;~sil
p ~ n r l i t i a nm r n u n j u k k a n biihwa keienjar parotis s:ipi be~nsifi~t
serous rnurrii. Kctlenj;~rsul)m;lntlil,~~l;~ris
bagian nsinarnyn terdiri atas sel-sel serous dnn sel-sel mukous. Sel-sel :i:;i~lnr.irlukr,us tt?rlihat lellih
domin;in ,jik;i ciil~andingkandengan sel-sel asinar serous. Sel-sel asinar kelenjnr pa~qc~tis
s:ipi bt'rr;lksi
positifd(5ngnn ii-ltcnsit;is st!dang (++i
tcn-hadap pewarnaan imunohistokirnia. Sel-sel epitel duktus ktllthrGnr
hcrcnksi scdnng ( + + smnpai dcngnn k u a t ( + + + I , Pnla sebaran lisozinl yang terdeteksi pada pent:!ili;in ini
n ~ r n u ~ ? j u k k ukcmiripan
n
dcngan hcwan mamalia pada umumnya. Sel-sel asinnr serous pad;] ke1rnj:ir
submandibularis rllcmbcrikan rcaksi positif dcngan ill tcnsistas rcndah (+) san-lpai dengan sedang ( + + )
dnn sel-scl epitel d u k t u s kclcnjnr dcngan intcnsitas kuat (+++ ). 1,isoxim ditemukan pada bagian s ~ l - s e l
asirlnr serous dnn sel-sel epitcl duktris kclcrljar parolis dnn submandibularis.
Kata kunci: irnunohistokimia, lisozim, kclanjar lurinl1
I,y*o/.irnrl
. ..
is ~ i i ~ ) i ~of
l ~i ~l tl d! u c i n pdirect bacteriolytic action by hydrolyzing the glycosidc bonds found
in b;lcterial cell wall. A study is thrr.~for.ecorlductetl to detect the lysozymc-producing cells in thc bovine
s:iliv:~rygl:ln(ls. In thi4 study, tissue prepnr.:~tionwas rxaminerl using histolofi-lcaland imn~unol~istochcmicnl
staining. 'Yhr! results showed that bvvirle parotid glands were purely serous acinar*cclls. The s u b m ; ~ n d i h u l n ~
t;rl;111tl,however, contained both rllucous and st:rous :ir:in:ir cells, but the mucous ~ c i n acr d l s were found
to hc I I I O ~ Cdominant t h a n the serous ncinnr cells. Immunrihistochemica1 staining on parotid gland using
antik~otly;ig;linst lyzozirne gave a moderate positive: rt?ilct.ion(++-Ito the acinnr cells and n strong positive
1 +++ 1 to the cpithcliill cclls of the ducts. The distr.ibution ~):~ttctrn
of lyst~zylneproducing crlls urns
~+cnction
similar to tllose of otlzcr mammalian species. In the submnndiliuli~i-ygl;intl, ;I weak i+)t o mediurn ( + + )
positive reaction was drtcctrd in the :tcinnr serous cclls, hilt n .strr>ng11,rwit i v ~
react,ion I +++) ivns dtltclvteri
i n the cpithclinl cclls of t l i r l tlucts. I A y s o Z ~ m
was
c ibun(l 111 t l i r . stht.r,rl:: :~r:in;-11.
~ ~ 1i-tncl
1 s rpithc1i;il rt.115 of the
r l t l r t s in 110th parotid :inti sul)rn:lnriil~ul;l~.y
gl;~ncls.
K c y Word: I ~ n m u n o l ~ i s t o c h c : ~ ~I.yso;lym~,
~ i c i ~ l , S:3li1,;1r.ygliind;:
?Turna1 Veteriner, Mare t 2007
10