Keterangan : y = nilai absorbansi x = konsentrasi asam askorbat

  Perpustakaan Unika LAMPIRAN Lampiran 1. KURVA STANDAR ASAM ASKORBAT

  Konsentrasi asam askorbat (ppm) 8 16 20 24

  32

  40 Absorbansi 1 0,5017 0,3582 0,2925 0,2158 0,0861 0,0133 Absorbansi 2 0,5514 0,3682 0,2993 0,2308 0,0798 0,0182 Absorbansi 3 0,5159 0,3842 0,3185 0,2467 0,1199 0,0221

  0.6 y = -0.016x + 0.6307

  2 R = 0.9815

  0.5

  0.4 si

  0.3 an rb

  0.2 so b a

  0.1

  5

  10

  15

  20

  25

  30

  35

  40

  45

• 0.1

  

konsentrasi asam askorbat

Berdasarkan hasil pembuatan kurva standar untuk analisa asam askorbat, diperoleh persamaan sebagai berikut: y = -0,016x +0,6307

  2 dengan R = 0,9815 Keterangan : y = nilai absorbansi x = konsentrasi asam askorbat

  Perpustakaan Unika Lampiran 2. KURVA STANDAR TOTAL FENOL

  Konsentrasi quercetin (ppm) 10 20 30 40 50 60 70 80 90 Absorbansi 0,0875 0,2574 0,4530 0,4949 0,6561 0,6946 0,8031 0,9111 1,0314

  1 Absorbansi 0,1501 0,2612 0,3856 0,4930 0,6531 0,7158 0,8203 0,9248 1,0640

  2 Absorbansi 0,0930 0,2850 0,3861 0,4847 0,6514 0,7205 0,8206 0,9315 1,0441

  3

  y = 0.0112x + 0.041

  kurva standar total fenol

  2 R = 0.9892

  1.2

  1

  i s

  0.8

  an

  0.6

  rb o s

  0.4

  ab

  0.2

  20

  40

  60 80 100

  Konsent

rasi quercetin (ppm)

  Berdasarkan hasil pembuatan kurva standar untuk analisa total fenol, diperoleh persamaan sebagai berikut: y = 0,0112x + 0,041

  2 dengan R = 0,9892 Keterangan : y = nilai absorbansi x = konsentrasi quercetin

  Perpustakaan Unika Lampiran 3. HASIL OBSERVASI METODE PEMASAKAN PADA 5 RUMAH MAKAN Tgl Nama Jns Pemasakan Jns Sayur Jumlah Jumlah Jumlah Proses Pemasakan Rumah Makan Sayur Air Minyak

  09-10-09 Agathis, Perebusan Kol putih 55 g 250 ml Sayur dimasukkan ke air mendidih o

  Plamongan Indah - Cap Cay kuah Kembang kol 80 g (±100

  C) dan dimasak selama 4 menit Sawi hijau 50 g 44 detik 16-10-09 Sawi Steamboat Perebusan putih 100 g 500 ml Sayur dimasukkan ke dalam air o

  House, DP Mall - steamboat Kembang kol 30 g mendidih (98

  C) dan direbus selama Sawi sendok 30 g 2-3 menit 17-10-09 Kelapa Gading, Penumisan Kailan ±50-60 g/ ±100 ml ± 50 ml sebelum ditumis, Kailan direbus ± 3 Sawi Jl. Gajah Mada - Ca aneka sayur putih jenis sayur menit dalam ± 220 ml air mendidih. Kembang kol Selanjutnya, seluruh sayuran ditumis o

  Sawi hijau selama 53 detik (suhu produk: 88

  C) Kembang Perebusan kol ± 40 g/ ±440 ml Sayuran dimasukkan ke dalam air o

• Cap cay kuah jenis sayur mendidih (±100

  C) dan dimasak Sawi putih selama 2 menit Sawi hijau o

  (suhu produk: 91

  C) Kol Brokoli 28-11-09 Ratna Berkah, Penumisan Sawi sendok 220 g ±180 ml ± 25 ml Sayuran dimasukkan ke dalam

  Gombel - ca sawi sendok minyak panas, lalu ditumis selama ± 1,5-2 menit (suhu produk: 83,3

  C) Penumisan Brokoli ± 130 g ±180 ml ± 25 ml Sayuran dimasukkan ke dalam

• ca brokoli + sayuran lain hingga ± minyak panas, kemudian ditumis (jamur, wortel, 200 g selama ± 1,5-2 menit (suhu produk: kapri)

  82,1

  C) 11-12-09 Mitra Keluarga, Penumisan sawi sendok ± 160 g ±430 ml ±15 ml Mula-mula sawi sendok direbus Bukit Sari - ca sawi sendok (untuk dalam air mendidih selama 30 detik; merebus) Kemudian baru ditumis selama 45 detik (suhu produk: 75

  C)

  50

  Perpustakaan Unika Lampiran 4. HASIL UJI HARDNESS BEBERAPA SAYURAN BRASSICA

  Perlakuan Rebus 2 menit Kukus 2 menit Kukus 3 menit Kukus 4 menit Hardness Sawi 2153,04 ± 2323,79 ± 2481,81 ± 2320,71± a a a a sendok (gf) 202,74 549,96 251,41 361,89 3625,23 ± 4712, 89 ± 4270,03 ± 4798,02 ± Hardness a b ab b

  Brokoli (gf) 632,93 845, 63 1349,71 1070,88 Hardness Sawi 1472, 41 ± 1266,30 ± 1458,23 ± 2454,27 ± a a a b

  Hijau (gf) 661,52 498,11 570,84 572,12 Hardness Kol 2236,09 ± 3593,30 ± 2758,88 ± 3570,24 ± a a a a

  (gf) 1780,87 1559,35 1128,92 871,97 Hardness Sawi 1789,28 ± 2160,57 ± 2210,46 ± 2308,77 ± a b b b

  Putih (gf) 464,95 325,67 444,56 163,57 Semua nilai merupakan mean ± standar deviasi

  ● ● Nilai dengan superscript yang berbeda antar kolom menunjukkan adanya perbedaan nyata antar perlakuan (p

  ≤ 0,05) dengan menggunakan uji wilayah Duncan

LAMPIRAN 5. ANALISA VARIANSI ONE-WAY TERHADAP HARDNESS BEBERAPA SAYURAN BRASSICA

  Total 5766256.362

  Perpustakaan Unika

  .185 .304 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 9.000.

  2 rebus 2 menit 9 3625.224777 kukus 3 menit 9 4270.028139 4270.028139 kukus 2 menit 9 4712.892181 kukus 4 menit 9 4798.015619 Sig.

  1

  Perlakuan N

  Duncan Subset for alpha = .05

   brokoli

  .090 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 9.000.

  1 rebus 2 menit 9 2153.037504 kukus 4 menit 9 2320.709569 kukus 2 menit 9 2323.790351 kukus 3 menit 9 2481.809724 Sig.

  Subset for alpha = .05 Perlakuan N

  35 Sawi sendok Duncan

  

ANOVA

Sum of Squares df Mean Square F Sig.

  Sawi sendok Between Groups 486662.628 3 162220.876 1.207 .323

  35 Sawi putih Between Groups 1393259.237 3 464419.746 3.398 .030

  Total 72916176.291

  Within Groups 61102770.738 32 1909461.586

  35 Kol Between Groups 11813405.553 3 3937801.851 2.062 .125

  Total 18466795.996

  Within Groups 10711239.567 32 334726.236

  35 Sawi hijau Between Groups 7755556.428 3 2585185.476 7.723 .001

  Total 40450456.210

  Within Groups 32673611.428 32 1021050.357

  35 brokoli Between Groups 7776844.782 3 2592281.594 2.539 .074

  Total 4788501.444

  Within Groups 4301838.816 32 134432.463

  Within Groups 4372997.125 32 136656.160

  53 Perpustakaan Unika sawi hijau

  Duncan Subset for alpha = .05

  Perlakuan N

  1

  2 kukus 2 menit 9 1266.301864 kukus 3 menit 9 1458.232525 rebus 2 menit 9 1472.409679 kukus 4 menit 9 2454.267873 Sig.

  .483 1.000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 9.000.

   kol

  Duncan Subset for alpha = .05

  Perlakuan N

  1 rebus 2 menit 9 2236.086059 kukus 3 menit 9 2758.875863 kukus 4 menit 9 3570.236999 kukus 2 menit 9 3593.300551 Sig.

  .064 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 9.000.

   sawi putih

  Duncan Subset for alpha = .05

  Perlakuan N

  1

  2 rebus 2 menit 9 1789.280786 kukus 2 menit 9 2160.570167 kukus 3 menit 9 2210.459620 kukus 4 menit 9 2308.765295 Sig.

  1.000 .430 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 9.000.

  LAMPIRAN 6. ANALISA NORMALITAS DATA PENGUJIAN KOMPONEN KIMIAWI SAWI PUTIH DAN SAWI SENDOK Tests of Normality

  Kukus .204 6 .200(*)

  Tumis .121 18 .200(*) * This is a lower bound of the true significance. a Lilliefors Significance Correction

  Kukus .196 18 .067

  Rebus .144 18 .200(*)

  Kadar air sawi putih Segar .197 18 .063

  Tumis .299 6 .101

  Kukus .293 6 .116

  Rebus .312 6 .069

  Fenol sawi putih Segar .298 6 .105

  Tumis .224 6 .200(*)

  Kukus .277 6 .169

  Rebus .284 6 .142

  Askorbat sawi putih Segar .187 6 .200(*)

  Tumis .266 6 .200(*)

  Rebus .151 6 .200(*)

  Kolmogorov-Smirnov(a) Perlakuan Statistic df Sig.

  AO sawi putih Segar .273 6 .184

  Tumis .138 18 .200(*)

  Kukus .199 18 .058

  Rebus .125 18 .200(*)

  Kadar air sawi sendok Segar .109 18 .200(*)

  Fenol sawi sendok segar .246 6 .200(*) rebus .289 6 .129 kukus .219 6 .200(*) tumis .175 6 .200(*)

  Tumis .242 6 .200(*)

  Kukus .156 6 .200(*)

  Rebus .225 6 .200(*)

  Askorbat sawi sendok Segar .303 6 .091

  Tumis .199 6 .200(*)

  Kukus .316 6 .062

  Rebus .243 6 .200(*)

  AO sawi sendok Segar .214 6 .200(*)

  Perpustakaan Unika

LAMPIRAN 7. ANALISA VARIANSI ONE-WAY DATA PENGUJIAN

  Homogeneous Subsets AO sawi sendok

  fenol sawi sendok Perpustakaan Unika

  1.000 .492 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 6.000.

  2 segar 6 26.1642 tumis 6 29.4733 rebus 6 29.9342 kukus 6 30.0242 Sig.

  1

  Perlakuan N

  Duncan Subset for alpha = .05

  askorbat sawi sendok

  1.000 1.000 .080 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 6.000.

  3 segar 6 31.6219 rebus 6 38.9802 tumis 6 52.4310 kukus 6 56.5152 Sig.

  2

  1

  Perlakuan N

  Duncan Subset for alpha = .05

  

KOMPONEN KIMIAWI PADA SAWI SENDOK

ANOVA

  Sum of Squares df

  Total 1538.902

  Within Groups 37.756 68 .555

  23 kdr_air_sawi_sendok Between Groups 1501.145 3 500.382 901.196 .000

  Total 1487613.084

  Within Groups 185232.883 20 9261.644

  23 Fenol sawi sendok Between Groups 1302380.201 3 434126.734 46.874 .000

  Total 93.958

  Within Groups 33.078 20 1.654

  23 Askorbat sawi sendok Between Groups 60.880 3 20.293 12.270 .000

  2711.267

  293.392 20 14.670 Total

  2417.875 3 805.958 54.941 .000 Within Groups

  Mean Square F Sig. AO sawi sendok Between Groups

  71 Post Hoc Tests

  56 Perpustakaan Unika

  Duncan Subset for alpha = .05

  Perlakuan N

  1

  2

  3 rebus 6 881.6042 tumis 6 1252.7232 kukus 6 1312.5030 segar 6 1528.5417 Sig.

  1.000 .295 1.000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 6.000.

  kdr air sawi sendok

  Duncan Subset for alpha = .05 perlakuan N

  1

  2

  3 tumis 18 83.9365 segar 18 94.2049 kukus 18 94.3496 rebus 18 94.8469 Sig.

  1.000 .562 1.000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 18.000.

  Perpustakaan Unika

LAMPIRAN 8. ANALISA VARIANSI ONE-WAY DATA PENGUJIAN KOMPONEN KIMIAWI PADA SAWI PUTIH ANOVA

  Sum of Mean Squares df Square F Sig. AO sawi putih Between Groups

  96.561 3 32.187 6.213 .004 Within Groups

  103.613 20 5.181 Total

  200.174

  23 Askorbat sawi putih Between Groups 205.292 3 68.431 11.129 .000

  Within Groups 122.982 20 6.149

  Total 328.273

  23 Fenol sawi putih Between Groups 66168.042 3 22056.014 2.225 .117

  Within Groups 198229.150 20 9911.458

  Total 264397.192

  23 Kdr air sawi putih Between Groups 1496.528 3 498.843 2278.149 .000

  Within Groups 14.890 68 .219

  Total 1511.418

  71 Post Hoc Tests

  Homogeneous Subsets AO sawi putih

  Duncan Subset for alpha = .05

  Perlakuan N

  1

  2 rebus 6 17.6949 segar 6 18.1468 kukus 6 21.5112 tumis 6 22.2583 Sig.

  .735 .576 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 6.000.

  askorbat sawi putih

  Duncan Subset for alpha = .05

  Perlakuan N

  1

  2

  3 segar 6 16.2217 rebus 6 18.6192 18.6192 tumis 6 21.4725 21.4725 kukus 6 23.9858 Sig.

  .110 .060 .094 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 6.000.

  58 Perpustakaan Unika fenol sawi putih

  Duncan Subset for alpha = .05

  Perlakuan N

  1

  2 rebus 6 262.3631 tumis 6 330.1309 330.1309 segar 6 372.9524 372.9524 kukus 6 401.8929 Sig.

  .082 .251 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 6.000.

  kdr air sawi putih

  Duncan Subset for alpha = .05 perlakuan N

  1

  2

  3 tumis 18 86.0464 kukus 18 96.3846 segar 18 96.4063 rebus 18 96.9020 Sig.

  1.000 .890 1.000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 18.000.

  Perpustakaan Unika LAMPIRAN 9. ANALISA KORELASI AKTIVITAS ANTIOKSIDAN, ASAM ASKORBAT, DAN TOTAL FENOL PADA SAWI SENDOK ► Sawi sendok Segar Correlations

  AO sendok Askorbat Fenol sendok segar sendok segar segar AO sendok segar Pearson Correlation 1 .532 -.636

  Sig. (2-tailed) .278 .174

  N

  6

  6

  6 Askorbat sendok segar Pearson Correlation .532 1 -.919(**)

  Sig. (2-tailed) .278 .009

  N

  6

  6

  6 Fenol sendok segar Pearson Correlation

• .636 -.919(**)

  1 Sig. (2-tailed) .174 .009

  N

  6

  6

  6 ** Correlation is significant at the 0.01 level (2-tailed).

  ► Sawi sendok rebus Correlations

  AO sendok Askorbat Fenol sendok rebus sendok rebus rebus AO sendok rebus Pearson Correlation 1 -.204 -.345

  Sig. (2-tailed) .698 .503

  N

  6

  6

  6 Askorbat sendok rebus Pearson Correlation

• .204 1 .844(*)

  Sig. (2-tailed) .698 .034

  N

  6

  6

  6 Fenol sendok rebus Pearson Correlation

• .345 .844(*)

  1 Sig. (2-tailed) .503 .034

  N

  6

  6

  6 * Correlation is significant at the 0.05 level (2-tailed).

  ► Sawi sendok kukus Correlations

  AO sendok Askorbat Fenol sendok kukus sendok kukus kukus AO sendok kukus Pearson Correlation 1 .113 .175

  Sig. (2-tailed) .831 .740

  N

  6

  6

  6 Askorbat sendok kukus Pearson Correlation .113 1 -.814(*)

  Sig. (2-tailed) .831 .049

  N

  6

  6

  6 Fenol sendok kukus Pearson Correlation .175 -.814(*)

  1 Sig. (2-tailed) .740 .049

  N

  6

  6

  6 * Correlation is significant at the 0.05 level (2-tailed).

  60 Perpustakaan Unika

  ► Sawi sendok tumis Correlations

  AO sendok Askorbat Fenol sendok tumis sendok tumis tumis AO sendok tumis Pearson Correlation 1 -.132 -.046

  Sig. (2-tailed) .804 .931

  N

  6

  6

  6 Askorbat sendok tumis Pearson Correlation

• .132 1 -.443

  Sig. (2-tailed) .804 .379

  N

  6

  6

  6 Fenol sendok tumis Pearson Correlation

• .046 -.443

  1 Sig. (2-tailed) .931 .379

  N

  6

  6

  6

  Perpustakaan Unika LAMPIRAN 10. ANALISA KORELASI AKTIVITAS ANTIOKSIDAN, ASAM ASKORBAT, DAN TOTAL FENOL PADA SAWI PUTIH ► Sawi putih Segar Correlations

  AO putih Askorbat putih Fenol putih segar segar segar AO putih segar Pearson Correlation 1 -.335 .101

  Sig. (2-tailed) .516 .849

  N

  6

  6

  6 Askorbat putih segar Pearson Correlation

• .335 1 -.494

  Sig. (2-tailed) .516 .319

  N

  6

  6

  6 Fenol putih segar Pearson Correlation .101 -.494

  1 Sig. (2-tailed) .849 .319

  N

  6

  6

  6

  ► Sawi putih rebus Correlations

  AO putih Askorbat putih Fenol putih rebus rebus rebus AO putih rebus Pearson Correlation 1 -.653 .590

  Sig. (2-tailed) .160 .218

  N

  6

  6

  6 Askorbat putih rebus Pearson Correlation

• .653 1 -.987(**)

  Sig. (2-tailed) .160 .000

  N

  6

  6

  6 Fenol putih rebus Pearson Correlation .590 -.987(**)

  1 Sig. (2-tailed) .218 .000

  N

  6

  6

  6 ** Correlation is significant at the 0.01 level (2-tailed).

  ► Sawi putih kukus Correlations

  AO putih Askorbat putih Fenol putih kukus kukus kukus AO putih kukus Pearson Correlation 1 -.853(*) .883(*)

  Sig. (2-tailed) .031 .020

  N

  6

  6

  6 Askorbat putih kukus Pearson Correlation

• .853(*) 1 -.986(**)

  Sig. (2-tailed) .031 .000

  N

  6

  6

  6 Fenol putih kukus Pearson Correlation .883(*) -.986(**)

  1 Sig. (2-tailed) .020 .000

  N

  6

  6

  6 * Correlation is significant at the 0.05 level (2-tailed).

• Correlation is significant at the 0.01 level (2-tailed).

  62 Perpustakaan Unika

  ► Sawi putih tumis Correlations

  AO putih Askorbat putih Fenol putih tumis tumis tumis AO putih tumis Pearson Correlation 1 -.956(**) .959(**)

  Sig. (2-tailed) .003 .003

  N

  6

  6

  6 Askorbat putih tumis Pearson Correlation

• .956(**) 1 -.872(*)

  Sig. (2-tailed) .003 .023

  N

  6

  6

  6 Fenol putih tumis Pearson Correlation .959(**) -.872(*)

  1 Sig. (2-tailed) .003 .023

  N

  6

  6

  6 ** Correlation is significant at the 0.01 level (2-tailed).

• Correlation is significant at the 0.05 level (2-tailed).