THE ANTIBACTERIAL ACTIVITY OF BLACK CUMIN SEED (Nigella Sativa) INFUSION AGAINST Staphylococcus aureus AND Shigella flexneri IN VITRO.

ABSTRACT
Traditional medicine is an ancient medical practice that existed in human
societies before the application of modern science of health and is still in use today.
Although modern medicine is extensively spread, traditional medicine still mainstay
of about 75-80% of world populations, mainly in the developing countries such as
Asian and African countries, for primary health care. Black cumin seeds (Nigella
sativa) are an indigenous plant which is used empirically to treat range of hepatic and
digestive disorders. The Staphylococcus aureus and Shigella flexneri are etiology of
digestive disorders such as food poisoning and dysenteric diarrhea. Phytochemical
evaluation of the seed shows that its volatile oil contains thymoquinone which has an
antibacterial effect.
The objective of this research is to determine the antibacterial effect of black
cumin seed infusion against Staphylococcus aureus and Shigella flexneri. This study
is a experimental study. Infusion method is used in the experiment because it is an
easiest procedure, where everyone will able to do by themselves.
Result of the research showed there is no diameter of inhibition zone of 100%,
75%, 50%, and 25% of black cumin seed infusion.
In conclusion, after the microbial experiment was done the infusion of black
cumin seed is not proven has antibacterial effect against Staphylococcus aureus and
Shigella flexneri in vitro.

Keywords: Black Cumin seed infusion, Staphylococcus aureus, Shigella flexneri.

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