Sumber : Percobaan Hewan Laboratorium (Soehardjono, 1993 dalam Wibowo, 2009)

Lampiran A. Surat Rekomendasi Kode Etik Penelitian Kesehatan

Lampiran B. Tabel Konversi Perhitungan Dosis untuk Berbagai Jenis Hewan dan Manusia (berdasarkan luas permukaan tubuh)

Mencit Tikus Marmut Kelinci Kucing Kera Anjing Manusia 20 g 200g 400 g 2 kg 2 kg

4 Kg

12 Kg

70 Kg Mencit 1,0 7,0 12,25 27,8 29,7 64,1 124,2 387,9 20 g Tikus 0,14 1,0 1,74 3.9 4,2 9,2 17,8 56,0 200g Marmut 0,08 0,57 1,0 2,25 2,4 5,2 10,2 31,5 400 g Kelinci 0,04 0,25 0,44 1,0 1,08 2,4 4,5 14,2 2 kg Kucing 0,03 0,23 0,41 0,92 1,0 2,2 4,1 13,0 2 kg Kera 0,016 0,11 0,19 0,42 0,45 1,0 1,9 6,1

4 Kg Anjing 0,008 0,06 0,10 0,22 0,24 0,52 1,0 3,1

12 Kg Manusia 0,0036 0,018 0,031 0,07 0,076 0,16 0,32 1,0

70 Kg

Sumber : Percobaan Hewan Laboratorium (Soehardjono, 1993 dalam Wibowo, 2009)

Lampiran C. Penentuan Dosis Aloksan

Pada peneliatian ini dengan menggunakan metode Etuk (2009), yaitu dengan menggunakan aloksan dengan dosis 180 mg/kg bb pada tikus untuk menginduksi diabetes melitus.

Faktor konversi dosis untuk tikus dengan berat badan 200 g pada mencit dengan berat badan 20 g adalah 0,14. Dosis untuk mencit dengan berat 20 g adalah = =

= 36 mg x 0,14 = 5,04 mg

Lampiran D. Penentuan Dosis Ekstrak

Pada penelitian ini dengan menggunakan metode Lumban Raja (2009), yaitu

dengan menggunakan ekstrak etanol biji mahoni dengan dosis bertingkat, yaitu 50mg/kg bb, 100mg/kg bb, 150mg/kg bb yang diberikan pada tikus.

Faktor konversi dosis untuk tikus dengan berat badan 200 g, pada mencit

dengan berat badan 20 g adalah 0,14. Dosis 1 untuk mencit dengan 20 g = 1,4 mg =

= =10 mgx0,14

=1,4 mg

Dosis 2 untuk mencit dengan 20 g = 2,8 mg

= = =20 mgx0,14 =2,8 mg

Dosis 3 untuk mencit dengan 20 g = 4,2 mg = = =30 mgx0,14

=4,2 mg

Lampiran E. Volume maksimum larutan sediaan uji yang dapat diberikan pada beberapa spesies hewan coba (mL)

Intravena Intramuscular Intraperitoneal Sub cutan Per oral Mencit (20-30g) 0,5 0,05 1,0 1,5-1,0 1,0 Tikus (100g) 1,0 0,1 2,5 2,5 5,0 Hamster (50g) - 0,1 1-2 2,5 2,5 Marmot (250g) - 0,25 2-5 5,0 10,0 Merpati (300g) 2,0 0,5 2,0 2,0 10,0 Kelinci (2,5kg) 5-10 0,5 10-20 5-10 20,0 Kucing (3kg) 5-10 1,0 10-20 5-10 50,0 Anjing (5kg) 10-20 5,0 20-50 10,0 100,0 Sumber: (Ritschel,1974 dalam Sulastry, 2009))

Lampiran F. Data Mentah Hasil Pengamatan No. Perlakuan Berat Badan (gram)

18 P2U3 26,27 96 588 174

14 P1U4 29,88 63 212 170

15 P1U5 33,18 75 204 172

16 P2U1 29,84 95 223 142

17 P2U2 31,17 140 201 149

19 P2U4 32,81 78 220 477

20 P2U5 28,25 57 245 126

21 P3U1 26,67 64 299 195

22 P3U2 32,98 89 218 132

23 P3U3 28,12 91 230 141

13 P1U3 28,05 108 201 167

KGD

Puasa

(mg/dl)

Hari ke 1

KGD Diabetes (mg/dl) Hari ke 2 KGD Akhir (mg/dl) Hari ke

3 Jumlah Sel Raksasa

1 KNU1 29,61 112 122 114

2 KNU2 33,57 126 128 124

3 KNU3 35,74 134 130 141

4 KNU4 32,68 142 206 130

5 KNU5 27,47 130 141 129

6 KPU1 30,90 62 241 249

7 KPU2 29,06 92 329 328

8 KPU3 33,50 54 218 208

9 KPU4 35,11 79 248 263

10 KPU5 32,55 96 238 268

11 P1U1 30,99 58 227 226

12 P1U2 31,95 53 202 187

24 P3U4 34,46 53 266 172

25 P3U5 22,41 66 367 168

10 Lampiran G. Analisis Statistik Rerata Kadar gula Darah

Tests of Normality

Kolmogorov-Smirnov(a) Shapiro-Wilk Perlakuan Statistic Df Sig. Statistic Df Sig. KGD_H_1 KN

,200 5 ,200(*) ,971 5 ,879 KP

,200 5 ,200(*) ,920 5 ,531 P1

,249 5 ,200(*) ,852 5 ,200 P2

,264 5 ,200(*) ,946 5 ,710 P3

,254 5 ,200(*) ,883 5 ,322 * This is a lower bound of the true significance. a Lilliefors Significance Correction

Test of Homogeneity of Variance

Levene Statistic df1 df2 Sig. KGD_H_1 Based on Mean

,739

4 20 ,576 Based on Median

,503

4 20 ,734 Based on Median and with

,503 4 13,222 ,735 adjusted df Based on trimmed mean

,745

4 20 ,572

ANOVA

KGD_H_1 Sum of Squares Df Mean Square F Sig.

Between Groups 11665,040 4 2916,260 6,759 ,001

Within Groups 8629,200 20 431,460

Total 20294,240

24 P3 ,178 5 ,200(*) ,930 5 ,597 * This is a lower bound of the true significance. a Lilliefors Significance Correction

52,20000(*) 13,13712 ,007 -93,6266 -10,7734 P1
5,20000 13,13712 1,000 -46,6266 36,226
21,80000 13,13712 1,000 -63,2266 19,6266 P3
1,20000 13,13712 1,000 -42,6266 40,2266 P2 KN -35,60000 13,13712 ,135 -77,0266 5,8266
4,00000 13,13712 1,000 -45,4266 37,4266 P1 1,20000 13,13712 1,000 -40,2266 42,626
20,60000 13,13712 1,000 -62,0266 20,8266 * The mean difference is significant at the .05 level.

4,00000 13,13712 1,000 -37,4266 45,4266 P1 KN

P1 ,284 5 ,200(*) ,824 5 ,125

KP ,363 5 ,030 ,787 5 ,063

KGD_H_2 KN ,351 5 ,044 ,728 5 ,018

Perlakuan Kolmogorov-Smirnov(a) Shapiro-Wilk Statistic Df Sig. Statistic df Sig.

Tests of Normality

P3 KN

P3 20,60000 13,13712 1,000 -20,8266 62,0266

P1 21,80000 13,13712 1,000 -19,6266 63,2266

KP 16,60000 13,13712 1,000 -24,8266 58,0266

5,20000 13,13712 1,000 -36,2266 46,6266 P2 -16,60000 13,13712 1,000 -58,0266 24,8266 P3

Multiple Comparisons

56,20000(*) 13,13712 ,004 14,7734 97,6266 KP KN

35,60000 13,13712 ,135 -5,8266 77,0266 P3

57,40000(*) 13,13712 ,003 15,9734 98,8266 P2

KN KP 52,20000(*) 13,13712 ,007 10,7734 93,6266 P1

Lower Bound Upper Bound Lower Bound

Lower Bound Upper Bound

Difference (I- J) Std. Error Sig. 95% Confidence Interval

(I) Perlakuan (J) Perlakuan Mean

Dependent Variable: KGD_H_1 Bonferroni

P2 ,420 5 ,004 ,642 5 ,002

Test of Homogeneity of Variance

Levene Statistic df1 df2 Sig. KGD_H_2 Based on Mean

3,719

4 20 ,020 Based on Median

,748

4 20 ,571 Based on Median and with

,748 4 5,267 ,598 adjusted df Based on trimmed mean

2,776

4 20 ,055

Tests of Normality

Kolmogorov-Smirnov(a) Shapiro-Wilk Perlakuan Statistic Df Sig. Statistic df Sig. KGD_H_2 KN

,351 5 ,044 ,728 5 ,018 KP

,363 5 ,030 ,787 5 ,063 P1

,284 5 ,200(*) ,824 5 ,125 P2

,420 5 ,004 ,642 5 ,002 P3

,178 5 ,200(*) ,930 5 ,597 * This is a lower bound of the true significance. a Lilliefors Significance Correction

Test of Homogeneity of Variance

Levene Statistic df1 df2 Sig. KGD_H_2 Based on Mean

1,673

4 20 ,196 Based on Median

,548

4 20 ,702 Based on Median and with

,548 4 9,418 ,705 adjusted df Based on trimmed mean

1,350

4 20 ,286

Kruskal-Wallis Test Ranks

Perlakuan N Mean Rank KGD_H_2 KN 5 3,80

KP 5 17,90 P1 5 9,10 P2 5 15,30 P3 5 18,90 Total

25 Test Statistics(a,b) KGD_H_2

Chi-Square 15,146

4 Asymp. Sig. ,004 a Kruskal Wallis Test b Grouping Variable: Perlakuan

Mann-Whitney Test Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_2 KN 5 3,00 15,00

KP 5 8,00 40,00 Total

10 Test Statistics(b) KGD_H_2

Mann-Whitney U ,000

Wilcoxon W 15,000

2,611 Asymp. Sig. (2-tailed)

,009 Exact Sig. [2*(1-tailed

,008(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_2 KN 5 3,60 18,00

P1 5 7,40 37,00 Total

10 Test Statistics(b) KGD_H_2

Mann-Whitney U 3,000

Wilcoxon W 18,000

1,984 Asymp. Sig. (2-tailed)

,047 Exact Sig. [2*(1-tailed

,056(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_2 KN 5 3,20 16,00

P2 5 7,80 39,00 Total

Test Statistics(b)

KGD_H_2 Mann-Whitney U

1,000 Wilcoxon W

16,000 Z

2,402 Asymp. Sig. (2-tailed)

,016 Exact Sig. [2*(1-tailed

,016(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_2 KN 5 3,00 15,00

P3 5 8,00 40,00 Total

10 Test Statistics(b) KGD_H_2

Mann-Whitney U ,000

Wilcoxon W 15,000

2,611 Asymp. Sig. (2-tailed)

,009 Exact Sig. [2*(1-tailed

,008(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_2 KP 5 7,80 39,00

P1 5 3,20 16,00 Total

10 Test Statistics(b) KGD_H_2

Mann-Whitney U 1,000

Wilcoxon W 16,000

2,402 Asymp. Sig. (2-tailed)

,016 Exact Sig. [2*(1-tailed

,016(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan Perlakuan N Mean Rank Sum of Ranks

KGD_H_2 KP 5 6,00 30,00 P2 5 5,00 25,00 Total

10 Test Statistics(b) KGD_H_2

Mann-Whitney U 10,000

Wilcoxon W 25,000

,522 Asymp. Sig. (2-tailed)

,602 Exact Sig. [2*(1-tailed

,690(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_4 KP 5 5,10 25,50

P3 5 5,90 29,50 Total

10 Test Statistics(b) KGD_H_4

Mann-Whitney U 10,500

Wilcoxon W 25,500

,419 Asymp. Sig. (2-tailed)

,675 Exact Sig. [2*(1-tailed

,690(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_2 P1 5 4,30 21,50

P2 5 6,70 33,50 Total

10 Test Statistics(b) KGD_H_2

Mann-Whitney U 6,500

Wilcoxon W 21,500

1,257 Asymp. Sig. (2-tailed)

,209 Exact Sig. [2*(1-tailed ,222(a)

Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_2 P1 5 3,20 16,00

P3 5 7,80 39,00 Total

10 Test Statistics(b) KGD_H_2

Mann-Whitney U 1,000

Wilcoxon W 16,000

2,402 Asymp. Sig. (2-tailed)

,016 Exact Sig. [2*(1-tailed

,016(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_2 P2 5 4,80 24,00

P3 5 6,20 31,00 Total

10 Test Statistics(b) KGD_H_2

Mann-Whitney U 9,000

Wilcoxon W 24,000

,731 Asymp. Sig. (2-tailed)

,465 Exact Sig. [2*(1-tailed

,548(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Tests of Normality

Kolmogorov-Smirnov(a) Shapiro-Wilk Perlakuan Statistic df Sig. Statistic df Sig. KGD_H_16 KN

,203 5 ,200(*) ,976 5 ,914 KP

,256 5 ,200(*) ,952 5 ,753 P1

,294 5 ,184 ,781 5 ,057 P2

,405 5 ,007 ,661 5 ,004 P3 ,200 5 ,200(*) ,950 5 ,734 * This is a lower bound of the true significance. a Lilliefors Significance Correction

Test of Homogeneity of Variance

Levene Statistic df1 df2 Sig. KGD_H_16 Based on Mean

4,204

4 20 ,012 Based on Median

,888

4 20 ,489 Based on Median and with

,888 4 4,701 ,536 adjusted df Based on trimmed mean

3,137

4 20 ,037

Tests of Normality

Kolmogorov-Smirnov(a) Shapiro-Wilk Perlakuan Statistic df Sig. Statistic df Sig. KGD_H_16 KN

,203 5 ,200(*) ,976 5 ,914 KP

,256 5 ,200(*) ,952 5 ,753 P1

,294 5 ,184 ,781 5 ,057 P2

,405 5 ,007 ,661 5 ,004 P3

,200 5 ,200(*) ,950 5 ,734 * This is a lower bound of the true significance. a Lilliefors Significance Correction

Test of Homogeneity of Variance

Levene Statistic df1 df2 Sig. KGD_H_16 Based on Mean

3,016

4 20 ,042 Based on Median

,929

4 20 ,467 Based on Median and with

,929 4 5,395 ,511 adjusted df Based on trimmed mean

2,341

4 20 ,090

Kruskal-Wallis Test Ranks

Perlakuan N Mean Rank KGD_H_16 KN 5 3,90

KP 5 21,80 P1 5 15,10 P2 5 12,60 P3 5 11,60 Total

25 Test Statistics(a,b) KGD_H_16 Chi-Square 15,407

4 Asymp. Sig. ,004 a Kruskal Wallis Test b Grouping Variable: Perlakuan

Mann-Whitney Test Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_16 KN 5 3,00 15,00

KP 5 8,00 40,00 Total

10 Test Statistics(b) KGD_H_16

Mann-Whitney U ,000

Wilcoxon W 15,000

2,611 Asymp. Sig. (2-tailed)

,009 Exact Sig. [2*(1-tailed

,008(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_16 KN 5 3,00 15,00

P1 5 8,00 40,00 Total

10 Test Statistics(b) KGD_H_16

Mann-Whitney U ,000

Wilcoxon W 15,000

2,611 Asymp. Sig. (2-tailed)

,009 Exact Sig. [2*(1-tailed

,008(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_16 KN 5 3,60 18,00

P2 5 7,40 37,00 Total

Test Statistics(b)

KGD_H_16 Mann-Whitney U

3,000 Wilcoxon W

18,000 Z

1,984 Asymp. Sig. (2-tailed)

,047 Exact Sig. [2*(1-tailed

,056(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_16 KN 5 3,30 16,50

P3 5 7,70 38,50 Total

10 Test Statistics(b) KGD_H_16

Mann-Whitney U 1,500

Wilcoxon W 16,500

2,305 Asymp. Sig. (2-tailed)

,021 Exact Sig. [2*(1-tailed

,016(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_16 KP 5 7,80 39,00

P1 5 3,20 16,00 Total

10 Test Statistics(b) KGD_H_16

Mann-Whitney U 1,000

Wilcoxon W 16,000

2,402 Asymp. Sig. (2-tailed)

,016 Exact Sig. [2*(1-tailed

,016(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_16 KP 5 7,00 35,00

P2 5 4,00 20,00 Total

10 Test Statistics(b) KGD_H_16

Mann-Whitney U 5,000

Wilcoxon W 20,000

1,567 Asymp. Sig. (2-tailed)

,117 Exact Sig. [2*(1-tailed

,151(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_16 KP 5 8,00 40,00

P3 5 3,00 15,00 Total

10 Test Statistics(b) KGD_H_16

Mann-Whitney U ,000

Wilcoxon W 15,000

2,611 Asymp. Sig. (2-tailed)

,009 Exact Sig. [2*(1-tailed

,008(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_16 P1 5 6,40 32,00

P2 5 4,60 23,00 Total

Test Statistics(b)

KGD_H_16 Mann-Whitney U

8,000 Wilcoxon W

23,000 Z

,940 Asymp. Sig. (2-tailed)

,347 Exact Sig. [2*(1-tailed

,421(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_16 P1 5 6,50 32,50

P3 5 4,50 22,50 Total

10 Test Statistics(b) KGD_H_16

Mann-Whitney U 7,500

Wilcoxon W 22,500

1,048 Asymp. Sig. (2-tailed)

,295 Exact Sig. [2*(1-tailed

,310(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Ranks

Perlakuan N Mean Rank Sum of Ranks KGD_H_16 P2 5 5,60 28,00

P3 5 5,40 27,00 Total

10 Test Statistics(b) KGD_H_16

Mann-Whitney U 12,000

Wilcoxon W 27,000

,104 Asymp. Sig. (2-tailed)

,917 Exact Sig. [2*(1-tailed

1,000(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Lampiran H. Analisis Statistik Rerata Berat Badan Tests of Normality

Kolmogorov-Smirnov(a) Shapiro-Wilk Perlakuan Statistic df Sig. Statistic df Sig. Berat_badan KN

,204 5 ,200(*) ,969 5 ,867 KP

,155 5 ,200(*) ,990 5 ,980 P1

,137 5 ,200(*) ,990 5 ,981 P2

,127 5 ,200(*) ,992 5 ,987 P3

,197 5 ,200(*) ,954 5 ,764 * This is a lower bound of the true significance. a Lilliefors Significance Correction

Test of Homogeneity of Variance

Levene Statistic df1 df2 Sig. Berat_badan Based on Mean

1,840

4 20 ,161 Based on Median

1,137

4 20 ,368 Based on Median and with

1,137 4 13,449 ,381 adjusted df Based on trimmed mean

1,867

4 20 ,156

ANOVA

Berat_badan Sum of Squares Df Mean Square F Sig.

Between Groups 38,900 4 9,725 ,967 ,447

Within Groups 201,206 20 10,060

Total 240,106

Lampiran I. Analisis Statistik Tingkat Perbandingan Jumlah sel Raksasa NPar Tests Kruskal-Wallis Test Ranks

NPar Tests Mann-Whitney Test Ranks

10 Test Statistics(b) Jumlah_Giant_

,151(a) a Not corrected for ties. b Grouping Variable: Perlakuan

,130 Exact Sig. [2*(1-tailed Sig.)]

20,500 Z

5,500 Wilcoxon W

Cell Mann-Whitney U

KP 5 6,90 34,50 Total

Perlakuan N Mean Rank Jumlah_Giant_Cell KN 5 4,50

Perlakuan N Mean Rank Sum of Ranks Jumlah_Giant_Cell KN 5 4,10 20,50

4 Asymp. Sig. ,067 a Kruskal Wallis Test b Grouping Variable: Perlakuan

8,784 Df

Cell Chi-Square

25 Test Statistics(a,b) Jumlah_Giant_

KP 5 13,50 P1 5 15,70 P2 5 15,60 P3 5 15,70 Total

1,514 Asymp. Sig. (2-tailed)

NPar Tests Mann-Whitney Test Ranks

Perlakuan N Mean Rank Sum of Ranks Jumlah_Giant_Cell KN 5 3,00 15,00

P1 5 8,00 40,00 Total

10 Test Statistics(b) Jumlah_Giant_

Cell Mann-Whitney U

,000 Wilcoxon W

15,000 Z

2,652 Asymp. Sig. (2-tailed)

,008 Exact Sig. [2*(1-tailed

,008(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

NPar Tests Mann-Whitney Test Ranks

Perlakuan N Mean Rank Sum of Ranks Jumlah_Giant_Cell KN 5 3,40 17,00

P2 5 7,60 38,00 Total

10 Test Statistics(b) Jumlah_Giant_

Cell Mann-Whitney U

2,000 Wilcoxon W

17,000 Z

2,227 Asymp. Sig. (2-tailed)

,026 Exact Sig. [2*(1-tailed

,032(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

NPar Tests Mann-Whitney Test Ranks

Perlakuan N Mean Rank Sum of Ranks Jumlah_Giant_Cell KN 5 3,00 15,00

P3 5 8,00 40,00 Total

10 Test Statistics(b) Jumlah_Giant_

Cell Mann-Whitney U

,000 Wilcoxon W

15,000 Z

2,660 Asymp. Sig. (2-tailed)

,008 Exact Sig. [2*(1-tailed

,008(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

NPar Tests Mann-Whitney Test Ranks

Perlakuan N Mean Rank Sum of Ranks Jumlah_Giant_Cell KP 5 5,30 26,50

P1 5 5,70 28,50 Total

10 Test Statistics(b) Jumlah_Giant_

Cell Mann-Whitney U

11,500 Wilcoxon W

26,500 Z

,210 Asymp. Sig. (2-tailed)

,834 Exact Sig. [2*(1-tailed

,841(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

NPar Tests Mann-Whitney Test Ranks

Perlakuan N Mean Rank Sum of Ranks Jumlah_Giant_Cell KP 5 5,10 25,50

P2 5 5,90 29,50 Total

10 Test Statistics(b) Jumlah_Giant_

Cell Mann-Whitney U

10,500 Wilcoxon W

25,500 Z

,419 Asymp. Sig. (2-tailed)

,675 Exact Sig. [2*(1-tailed ,690(a)

Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

NPar Tests Mann-Whitney Test Ranks

Perlakuan N Mean Rank Sum of Ranks Jumlah_Giant_Cell KP 5 5,20 26,00

P3 5 5,80 29,00 Total

10 Test Statistics(b) Jumlah_Giant_

Cell Mann-Whitney U

11,000 Wilcoxon W

26,000 Z

,317 Asymp. Sig. (2-tailed)

,751 Exact Sig. [2*(1-tailed

,841(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

NPar Tests Mann-Whitney Test Ranks

Perlakuan N Mean Rank Sum of Ranks Jumlah_Giant_Cell P1 5 5,40 27,00

P2 5 5,60 28,00 Total

10 Test Statistics(b) Jumlah_Giant_

Cell Mann-Whitney U

12,000 Wilcoxon W

27,000 Z

,105 Asymp. Sig. (2-tailed)

,916 Exact Sig. [2*(1-tailed

1,000(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

NPar Tests Mann-Whitney Test Ranks

Perlakuan N Mean Rank Sum of Ranks Jumlah_Giant_Cell P1 5 5,60 28,00

P3 5 5,40 27,00

Total

10 Test Statistics(b) Jumlah_Giant_

Cell Mann-Whitney U

12,000 Wilcoxon W

27,000 Z

,106 Asymp. Sig. (2-tailed)

,915 Exact Sig. [2*(1-tailed

1,000(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

NPar Tests Mann-Whitney Test Ranks

Perlakuan N Mean Rank Sum of Ranks Jumlah_Giant_Cell P2 5 5,50 27,50

P3 5 5,50 27,50 Total

10 Test Statistics(b) Jumlah_Giant_

Cell Mann-Whitney U

12,500 Wilcoxon W

27,500 Z

,000 Asymp. Sig. (2-tailed)

1,000 Exact Sig. [2*(1-tailed

1,000(a) Sig.)] a Not corrected for ties. b Grouping Variable: Perlakuan

Lampiran J. Alur Penelitian

Pemeliharaan mencit Puasa ± 16

Diberi diet standar Induksi aloksan 180 mg/kg BB Induksi

aloksan 180

mg/kg BB +

ekstrak

etanol biji

mahoni dosis

mg/kg BB

Induksi aloksan 180 mg/kg BB + ekstrak etanol biji mahoni dosis 100 mg/kg BB

Induksi aloksan 180 mg/kg BB + ekstrak etanol biji mahoni dosis 150 mg/kg BB

Pengukuran Kadar Gula darah Pengukuran Berat badan

Histologi limpa mencit

Lampiran K. Prosedur Pengolahan Ekstrak Biji Mahoni

Biji mahoni Dibersihkan dari kulit pembungkusnya Dikeringkan tidak pada sinar matahari secara langsung Dihaluskan Serbuk biji mahoni

Lampiran L. Prosedur Ekstrak Etanol Biji Mahoni

Serbuk biji mahoni Dimasukkan ke dalam botol berwarna gelap Ditambahkan pelarut etanol 96% Ditutup dan dibiarkan selama dua hari terlindung dari cahaya Disaring

Maserat Diuapkan menggunakan vakum putar pada suhu 40 C Dikeringkan Ekstrak Etanol biji mahoni

Lampiran M. Prosedur Pembuatan Suspensi Ekstrak Etanol Biji Mahoni

0,5 g CMC Ditaburkan dalam lumpang yang berisi aquades panas 10 ml Didiamkan 15 menit

Digerus hingga berbentuk gel

Gel CMC

Ekstrak etanol biji mahoni Ditambahkan gel CMC sedikit demi sedikit Digerus sampai terbentuk suspensi Dicukupkan volumenya sampai 100 ml dengan akuades

Larutan ekstrak

Lampiran N. Prosedur Pembuatan Preparat Histologis Limpa

Organ Limpa Difiksasi selama 1 hari

Dicuci dengan alkohol 70%

Didehidrasi dengan alkohol bertingkat

Dijernihkan dengan Xylol

Diinfiltrasi Ditanam dalam kotak kecil yang berisi parafin Dipotong pita parafin dengan menggunakan mikrotom

Direndam dalam air dingin

Direndam dalam air hangat

Ditempelkan pada objek gelas

Direkatkan dengan pemanasan di atas hot plate Dideparafinasi Diwarnai Ditutup dengan Canada balsam Preparat

Sumber : Percobaan Hewan Laboratorium (Soehardjono, 1993 dalam Wibowo, 2009)

Lampiran B. Tabel Konversi Perhitungan Dosis untuk Berbagai Jenis Hewan dan Manusia (berdasarkan luas permukaan tubuh)

Lampiran C. Penentuan Dosis Aloksan

Lampiran D. Penentuan Dosis Ekstrak

Lampiran E. Volume maksimum larutan sediaan uji yang dapat diberikan pada beberapa spesies hewan coba (mL)

Lampiran F. Data Mentah Hasil Pengamatan No. Perlakuan Berat Badan (gram)

Kruskal-Wallis Test Ranks

Mann-Whitney Test Ranks

Kruskal-Wallis Test Ranks

Mann-Whitney Test Ranks

Lampiran H. Analisis Statistik Rerata Berat Badan Tests of Normality

Lampiran I. Analisis Statistik Tingkat Perbandingan Jumlah sel Raksasa NPar Tests Kruskal-Wallis Test Ranks

NPar Tests Mann-Whitney Test Ranks

NPar Tests Mann-Whitney Test Ranks

NPar Tests Mann-Whitney Test Ranks

NPar Tests Mann-Whitney Test Ranks

NPar Tests Mann-Whitney Test Ranks

NPar Tests Mann-Whitney Test Ranks

NPar Tests Mann-Whitney Test Ranks

NPar Tests Mann-Whitney Test Ranks

NPar Tests Mann-Whitney Test Ranks

NPar Tests Mann-Whitney Test Ranks

Lampiran J. Alur Penelitian

Pemeliharaan mencit Puasa ± 16

Lampiran K. Prosedur Pengolahan Ekstrak Biji Mahoni

Lampiran L. Prosedur Ekstrak Etanol Biji Mahoni

Lampiran M. Prosedur Pembuatan Suspensi Ekstrak Etanol Biji Mahoni

Lampiran N. Prosedur Pembuatan Preparat Histologis Limpa

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