Institutional Repository | Satya Wacana Christian University: Aktivitas Antioksidan Ekstrak Fenolik Hasil Recovery Limbah Padat Jamu
Aktivitas Antioksidan Ekstrak Fenolik Hasil Recovery Limbah Padat Jamu
(Antioxidant Activity of Phenolic Extract From Recovery Yield of Solid Herb Waste)
Wening Galih Bhagawati*, Hartati Soetjipto**, A. Ign Kristijanto**
*) Mahasiswa Program Studi Kimia Fakultas Sains dan Matematika
**) Dosen Program Studi Kimia Fakultas Sains dan Matematika
Universitas Kristen Satya Wacana
Jalan Diponegoro 52 – 60, Salatiga – 50711
[email protected]
ABSTRACT
The purposes of this study were: firstly, to determined the content of phenolic compounds of
solid herb waste. Secondly, to determined its antioxidant activity and Sun Protection Factor
(SPF) value. The extraction has been conducted by maceration method using ethanol and ethyl
acetate with two levels of temperature (which were 270C and 500C) and three levels of
extraction time (which were 90, 120 and 150 minutes). The content, antioxidant activity and SPF
value of phenolic compounds were determined by spectrometric method.
The result of the study showed that: 1) the optimum phenolic compounds has obtained on
extraction condition of 270C and 90 minutes using ethanol solvent, with amount of 13.14 ± 0.87
mg GAE/gram sample, 2) the IC50 of ethanol extract has obtained on concentration of 5,000.67
ppm. Meanwhile, it had SPF value of 17.19 ± 0.01 on concentration of 900 µg/mL.
Keyword: antioxidant, phenolic, herb, SPF value, wastes
(Antioxidant Activity of Phenolic Extract From Recovery Yield of Solid Herb Waste)
Wening Galih Bhagawati*, Hartati Soetjipto**, A. Ign Kristijanto**
*) Mahasiswa Program Studi Kimia Fakultas Sains dan Matematika
**) Dosen Program Studi Kimia Fakultas Sains dan Matematika
Universitas Kristen Satya Wacana
Jalan Diponegoro 52 – 60, Salatiga – 50711
[email protected]
ABSTRACT
The purposes of this study were: firstly, to determined the content of phenolic compounds of
solid herb waste. Secondly, to determined its antioxidant activity and Sun Protection Factor
(SPF) value. The extraction has been conducted by maceration method using ethanol and ethyl
acetate with two levels of temperature (which were 270C and 500C) and three levels of
extraction time (which were 90, 120 and 150 minutes). The content, antioxidant activity and SPF
value of phenolic compounds were determined by spectrometric method.
The result of the study showed that: 1) the optimum phenolic compounds has obtained on
extraction condition of 270C and 90 minutes using ethanol solvent, with amount of 13.14 ± 0.87
mg GAE/gram sample, 2) the IC50 of ethanol extract has obtained on concentration of 5,000.67
ppm. Meanwhile, it had SPF value of 17.19 ± 0.01 on concentration of 900 µg/mL.
Keyword: antioxidant, phenolic, herb, SPF value, wastes