Institutional Repository | Satya Wacana Christian University: Aktivitas Antioksidan, Kandungan Fenolik Total dan Uji Organoleptik pada Tempe dengan Ekstrak Daun Beluntas (Pluchea indica L.)

Aktivitas Antioksidan, Kandungan Fenolik Total dan Uji Organoleptik pada
Tempe dengan Ekstrak Daun Beluntas (Pluchea indica L.)

(Antioxidant Activity, Total Phenolic Content, and Organoleptic Test in Tempe with
Marsh Fleabane Leaf Extract (Pluchea indica L.))
Panawidha Magatra 1*, Lusiawati Dewi 2** , dan Lydia Ninan Lestario 2**
1

Mahasiswa Program Studi Kimia, Fakultas Sains dan Matematika , Universitas Kristen Satya
Wacana, Salatiga, Indonesia
2
Dosen Program Studi Kimia, Fakultas Sains dan Matematika, Universitas Kristen Satya
Wacana, Salatiga, Indonesia
Jl. Diponegoro 52-60 Salatiga 50711
*Mobile : 085641523214, email: [email protected]

Abstract
This aims of this research were to determine the antioxidant activity and IC50 of
marsh fleabane leaf extract, established the total phenolic content, and conducted an
organoleptic test of tempe, in which the soybeans were soaked with marsh fleabane leaf
extract at various concentrations (0%, 1%, 3%, 4.5%, and 6%). The results showed that

the highest antioxidant activity was obtained from soaked tempe with a 4.5% marsh
fleabane leaf extract (90.96% ± 1.81) while the antioxidant activity of tempe without
marsh fleabane leaf extract was only 63,07% ± 4,65 and IC50 of marsh fleabane leaves
extract was 0.15g/100mL aquadest. The highest total phenolic content was obtained
from soaked tempe with a 6% marsh fleabane leaf extract (340,80 ± 40,33 mg/100g)
while total phenolic content of tempe without marsh fleabane leaves extract was only
129 mg/100 g ± 24.31. The results of the organoleptic test showed that the preferred
organoleptic test was obtained from tempe without marsh fleabane leaf extract (3.96 of
5). The preferred tempe, in which the soybeans had been soaked at various
concentrations with marsh fleabane leaf extract ,was 1.5% (3.14 of 5).

Keywords: antioxidant activity, total phenolic content, tempe, marsh fleabane leaves
(Pluchea indica L.)

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