Uji Efektivitas Anti-Aging dari Krim Ekstrak Bunga Brokoli (Brassica oleracea L var. Italica Plenck) pada Marmut
Lampiran 1. Hasil identifikasi tumbuhan
Lampiran 2. Gambar bunga brokoli kukus dan simplisia bunga brokoli
A B Keterangan:
A: Bunga brokoli kukus
B: Bunga brokoli kukus yang dirajang
Lampiran 2 (lanjutan)
A B Keterangan:
A: simplisia bunga brokoli (Brassica oleraceae flos)
B: serbuk simplisia bunga brokoli
Lampiran 3. Gambar mikroskopik serbuk simplisia bunga brokoli
Keterangan: 1.
Rambut penutup 2. Serbuk sari 3. Parenkim 4. Xilem dengan penebalan bentuk spiral 5. Kristal Ca oksalat bentuk prisma
Lampiran 4. Perhitungan pemeriksaan karakterisasi serbuk simplisia bunga
brokoli
1. Perhitungan kadar air volume (ml)
= x 100% % kadar air
berat sampel(g)
1. Berat Sampel : 5,0000 g Volume Air : 0, 4 ml
0,4 ml
% kadar air x 100% 8 %
= = 5,000 g
2. Berat Sampel : 5,0010 g Volume Air : 0, 4 ml
0,4 ml % kadar air = 7,99 % x 100% =
5,001 g
3. Berat Sampel : 5,012 g Volume Air : 0, 4 ml
0,4 m l
% kadar air = x 100% = 7,98 %
5,012 g 8%+7,99%+7,98%
= 7,99 %
% kadar air rata-rata =
3
2. Perhitungan kadar sari larut air berat sari (g)
100 100% x x
% kadar sari larut air =
berat simplisia (g)
20 1. Berat simplisia = 5,042 g
Berat sari = 0,2577 g 0,2577 100 x x 100% =
% kadar sari larut air = 25,55 % 5,042
20
Lampiran 4 (lanjutan) 2.
Berat simplisia = 5,018 g Berat sari = 0,2627 g
0,2627 100 26,17 % x x 100%
% kadar sari larut air = = 5,018
20 3. Berat simplisia = 5,014 g
Berat sari = 0,2497 g 0,2497 100 x =
% kadar sari larut air = x 100% 24,90 % 5,014
20
25,55%+26,17%+24,90% % kadar sari larut air rata-rata =
3
= 25,54 %
berat sari (g) 100 x x 100% % kadar sari larut etanol = berat simplisia (g)
20 1. Berat simplisia = 5,007 g
Berat sari = 0,1280 g 0,1280 100 x x 12,78 %
% kadar sari larut etanol = 100% = 5,007
20 2. Berat simplisia = 5,005g
Berat sari = 0,1343 g 0,1343 100 x x
% kadar sari larut etanol = 100% = 13,41 % 5,005
20
Lampiran 4 (lanjutan) 3.
% kadar abu total = 0,112 2,047 x 100% = 5,47 %
% kadar abu total rata-rata = 5,47%+6,27%+5,87%
100% = 5,87 %
% kadar abu total = 0,118 2,010 x
3. Berat simplisia = 2,010 g Berat abu = 0,118 g
0,126 2,008 x 100% = 6,27 %
2. Berat simplisia = 2,008 g Berat abu = 0,126 g % kadar abu total =
1. Berat simplisia = 2,047 g Berat abu = 0,112 g
Berat simplisia = 5,008 g Berat sari = 0,1193 g
% kadar abu total = berat abu (g) berat simplisia (g) x 100%
3 = 12,70 % 4. Perhitungan kadar abu total
% kadar sari larut etanol rata-rata = 12,78%+13,41%+11,91%
20 x 100% = 11,91 %
5,008 x 100
% kadar sari larut etanol = 0,1193
3 = 5,87 %
Lampiran 4 (lanjutan)
5. Perhitungan kadar abu tidak larut asam
berat abu (g) % kadar abu tidak larut asam = x 100% berat simplisia (g)
1. Berat simplisia = 2,047 g Berat abu = 0,003 g
0,003 x 100% = 0,146 % % kadar abu tidak larut asam =
2,047 2. Berat simplisia = 2,008 g
Berat abu = 0,003 g 0,003 x 100% = 0,149 %
% kadar abu tidak larut asam = 2,008 3.
Berat simplisia = 2,010 g Berat abu = 0,003 g
0,003 x
% kadar abu tidak larut dalam asam = 100% = 0,149 %
2,010 0,146%+0,149%+0,149% % kadar abu tidak larut asam rata -rata =
3 0,148 % =
Lampiran 5. Bagan kerja pembuatan serbuk simplisia bunga brokoli
Bunga brokoli dibersihkan dari pengotor dicuci hingga bersih dikukus selama 3 menit ditiriskan hingga tidak ada lagi air diiris bagian bakal bunga brokoli ditimbang
Bunga brokoli 3,4 kg dikeringkan di lemari pengering Simplisia kering 430 g dihaluskan
Serbuk simplisia karakterisasi simplisia
- Makroskopik - Mikroskopik - Kadar air
- Kadar sari yang larut dalam air
- Kadar sari yang larut dalam etanol
- Kadar abu total
- Kadar abu yang tidak larut dalam asam
Lampiran 6. Bagan kerja pembuatan ekstrak bunga brokoli
Serbuk Simplisia 300 g ditambah etanol 96% dimasukkan ke dalam bejana tertutup sekurangkurangnya selama 3 jam dimasukkan ke dalam perkolator didiamkan selama 24 jam
Simplisia dalam perkolator ditambahkan cairan penyari sampai satu lapis diatas serbuk ditutup dengan alumunium foil
Simplisia dan pelarut berwarna hijau tua dibuka kran perkolator dengan kecepatan pengaliran 1 ml/menit. Ekstrak etanol encer 5 L dipekatkan dengan alat rotary
evaporator pada suhu 50°C
Ekstrak etanol kental dimasukkan ke dalam alat freeze dryer Ekstrak kental 67,2 g
Lampiran 7. Gambar sediaan krim ekstrak bunga brokoli setelah selesai dibuat
A B C Keterangan:
A: Krim blanko
B: Krim ekstrak bunga brokoli 1%
C: Krim ekstrak bunga brokoli 3%
Lampiran 8. Gambar sediaan krim ekstrak bunga brokoli setelah 12 minggu
A B C Keterangan:
A: Krim blanko
B: Krim ekstrak bunga brokoli 1%
C: Krim ekstrak bunga brokoli 3%
Lampiran 9. Gambar alat penelitian
A B C Keterangan:
A: Lampu UV 366 nm
B: Alat moisture checker
C: Alat skin analyzer Aramo
Lampiran 10. Gambar hewan percobaan penelitian (marmut)
A B Keterangan:
A: Perawatan marmut
B: Marmut dengan punggung yang dicukur
Lampiran 11. Contoh pengukuran kondisi kulit marmut dengan skin analyzer
1.kadar air (moisture) Sebelum penyinaran Setelah penyinaran Pemulihan minggu I Pemulihan minggu II Pemulihan minggu III
Lampiran 11 (lanjutan)
Pemulihan minggu IV 2. kehalusan (evenness) dan besar pori (pore)
Sebelum penyinaran
Lampiran 11 (lanjutan)
Setelah penyinaran Pemulihan minggu I
Lampiran 11 (lanjutan)
Pemulihan minggu II Pemulihan minggu III
Lampiran 11 (lanjutan)
Pemulihan minggu IV 3.
Jumlah noda (spot) Sebelum penyinaran
Lampiran 11 (lanjutan)
Setelah penyinaran Pemulihan minggu I
Lampiran 11 (lanjutan)
Pemulihan minggu II Pemulihan minggu III
Lampiran 11 (lanjutan)
Pemulihan minggu IV 4.
Keriput (wrinkle) Sebelum penyinaran
Lampiran 11 (lanjutan)
Setelah penyinaran Pemulihan minggu I
Lampiran 11 (lanjutan)
Pemulihan minggu II Pemulihan minggu III
Lampiran 11 (lanjutan)
Pemulihan minggu IV
Lampiran 12. Data statistik hasil pengukuran kondisi kulit marmut 1.
FORMULA N Subset for alpha = .05
EKSTRAK BUNGA BROKOLI 3% 3 17,33 EKSTRAK BUNGA BROKOLI 1% 3 17,67 Sig.
1 BLANKO 3 12,00
2
FORMULA N Subset for alpha = .05
a
Tukey HSD
Setelah Penyinaran dengan UV
,707 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
1 BLANKO 3 31,67 EKSTRAK BUNGA BROKOLI 1% 3 32,33 EKSTRAK BUNGA BROKOLI 3% 3 32,33 Sig.
a
Kadar air (moisture)
8 Sebelum Penyinaran dengan UV Tukey HSD
8 P.Minggu IV Between Groups 40,222 2 20,111 13,923 ,006 Within Groups 8,667 6 1,444 Total 48,889
8 P. Minggu III Between Groups 136,222 2 68,111 11,145 ,010 Within Groups 36,667 6 6,111 Total 172,889
8 P. MingguII Between Groups 257,556 2 128,778 30,500 ,001 Within Groups 25,333 6 4,222 Total 282,889
8 P. Minggu I Between Groups 333,556 2 166,778 21,141 ,002 Within Groups 47,333 6 7,889 Total 380,889
8 Setelah UV Between Groups 60,667 2 30,333 34,125 ,001 Within Groups 5,333 6 ,889 Total 66,000
Within Groups 6,000 6 1,000 Total 6,889
Mean Square F Sig. SebelumUV Between Groups ,889 2 ,444 ,444 ,661
Sum of Squares df
ANOVA
1,000 ,903 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
Lampiran 12 (lanjutan) Pemulihan minggu I
Pemulihan minggu III
EKSTRAK BUNGA BROKOLI 1% 3 33,67 EKSTRAK BUNGA BROKOLI 3% 3 35,00 Sig.
1 BLANKO 3 30,00
2
Tukey HSD FORMULA N Subset for alpha = .05
Pemulihan minggu IV
1,000 ,361 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
EKSTRAK BUNGA BROKOLI 1% 3 29,00 EKSTRAK BUNGA BROKOLI 3% 3 32,00 Sig.
1 BLANKO 3 22,67
2
FORMULA N Subset for alpha = .05
a
Tukey HSD
,403 1,000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
Tukey HSD
EKSTRAK BUNGA BROKOLI 1% 3 21,33 EKSTRAK BUNGA BROKOLI 3% 3 31,33 Sig.
1 BLANKO 3 19,00
2
FORMULA N Subset for alpha = .05
a
Tukey HSD
Pemulihan minggu II
,154 1,000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
EKSTRAK BUNGA BROKOLI 1% 3 20,33 EKSTRAK BUNGA BROKOLI 3% 3 30,00 Sig.
1 BLANKO 3 15,33
2
FORMULA N Subset for alpha = .05
a
1,000 ,417 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
Lampiran 12 (lanjutan) 2.
a
1 BLANKO 3 42,67 EKSTRAK BUNGA BROKOLI 1% 3 45,00 EKSTRAK BUNGA BROKOLI 3% 3 49,33 Sig.
FORMULA N Subset for alpha = .05
a
Tukey HSD
Setelah Penyinaran dengan UV
,760 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
1 BLANKO 3 32,33 EKSTRAK BUNGA BROKOLI 1% 3 36,67 EKSTRAK BUNGA BROKOLI 3% 3 37,67 Sig.
FORMULA N Subset for alpha = .05
8 Sebelum Penyinaran dengan UV Tukey HSD
Kehalusan (evenness)
8 P.Minggu IV Between Groups 24,889 2 12,444 ,191 ,831 Within Groups 390,667 6 65,111 Total 415,556
8 P. Minggu III Between Groups 76,222 2 38,111 ,905 ,453 Within Groups 252,667 6 42,111 Total 328,889
8 P. MingguII Between Groups 89,556 2 44,778 ,993 ,424 Within Groups 270,667 6 45,111 Total 360,222
8 P. Minggu I Between Groups 48,222 2 24,111 ,838 ,478 Within Groups 172,667 6 28,778 Total 220,889
8 Setelah UV Between Groups 68,667 2 34,333 ,626 ,567 Within Groups 329,333 6 54,889 Total 398,000
Within Groups 490,000 6 81,667 Total 538,222
Mean Square F Sig. SebelumUV Between Groups 48,222 2 24,111 ,295 ,755
Sum of Squares df
ANOVA
,547 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
Lampiran 12 (lanjutan) Pemulihan minggu I
Tukey HSD
EKSTRAK BUNGA BROKOLI 3% 3 35,33 EKSTRAK BUNGA BROKOLI 1% 3 38,00 Sig.
1 BLANKO 3 34,00
FORMULA N Subset for alpha = .05
a
Tukey HSD
Pemulihan minggu IV
,498 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
3 35,00 EKSTRAK BUNGA BROKOLI 1% 3 41,00 Sig.
1 EKSTRAK BUNGA BROKOLI 3% 3 34,67 BLANKO
FORMULA N Subset for alpha = .05
a
Pemulihan minggu III
Tukey HSD
,457 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
3 36,67 EKSTRAK BUNGA BROKOLI 1% 3 43,00 Sig.
1 EKSTRAK BUNGA BROKOLI 3% 3 36,00 BLANKO
FORMULA N Subset for alpha = .05
a
Tukey HSD
Pemulihan minggu II
,448 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
3 40,00 EKSTRAK BUNGA BROKOLI 1% 3 43,00 Sig.
1 EKSTRAK BUNGA BROKOLI 3% 3 37,33 BLANKO
FORMULA N Subset for alpha = .05
a
,822 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
Lampiran 12 (lanjutan) 3.
FORMULA N
1 BLANKO 3 45,00 EKSTRAK BUNGA BROKOLI 3% 3 46,33 EKSTRAK BUNGA BROKOLI 1% 3 49,67 Sig.
FORMULA N Subset for alpha = .05
a
Tukey HSD
Setelah Penyinaran dengan UV
,064 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
3 12,00 EKSTRAK BUNGA BROKOLI 1% 3 23,00 Sig.
1 EKSTRAK BUNGA BROKOLI 3% 3 9,67 BLANKO
1 Subset for alpha = .05
a
Besar pori (pore)
8 Sebelum Penyinaran dengan UV Tukey HSD
8 P.Minggu IV Between Groups 96,222 2 48,111 1,473 ,302 Within Groups 196,000 6 32,667 Total 292,222
8 P. Minggu III Between Groups 272,667 2 136,333 3,229 ,112 Within Groups 253,333 6 42,222 Total 526,000
8 P. MingguII Between Groups 576,222 2 288,111 4,329 ,069 Within Groups 399,333 6 66,556 Total 975,556
8 P. Minggu I Between Groups 817,556 2 408,778 2,719 ,144 Within Groups 902,000 6 150,333 Total 1719,556
8 Setelah UV Between Groups 34,667 2 17,333 ,062 ,941 Within Groups 1679,333 6 279,889 Total 1714,000
Within Groups 194,667 6 32,444 Total 498,889
Mean Square F Sig. SebelumUV Between Groups 304,222 2 152,111 4,688 ,059
Sum of Squares df
ANOVA
,938 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
Lampiran 12 (lanjutan) Pemulihan minggu I
Pemulihan minggu III
1 EKSTRAK BUNGA BROKOLI 3% 3 9,67 BLANKO 3 13,33 EKSTRAK BUNGA BROKOLI 1% 3 17,67 Sig.
FORMULA N Subset for alpha = .05
a
Tukey HSD
Pemulihan minggu IV
,118 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
1 EKSTRAK BUNGA BROKOLI 3% 3 12,00 EKSTRAK BUNGA BROKOLI 1% 3 22,33 BLANKO 3 24,67 Sig.
FORMULA N Subset for alpha = .05
a
Tukey HSD
,065 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
Tukey HSD
1 EKSTRAK BUNGA BROKOLI 3% 3 12,33 EKSTRAK BUNGA BROKOLI 1% 3 26,00 BLANKO 3 31,33 Sig.
FORMULA N Subset for alpha = .05
a
Tukey HSD
Pemulihan minggu II
,127 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
3 41,67 Sig.
1 EKSTRAK BUNGA BROKOLI 3% 3 18,33 EKSTRAK BUNGA BROKOLI 1% 3 30,67 BLANKO
FORMULA N Subset for alpha = .05
a
,275 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
Lampiran 12 (lanjutan) 4.
FORMULA N Subset for alpha = .05
3 54,33 Sig.
1 EKSTRAK BUNGA BROKOLI 1% 3 49,33 EKSTRAK BUNGA BROKOLI 3% 3 54,00 BLANKO
FORMULA N Subset for alpha = .05
a
Tukey HSD
Setelah Penyinaran dengan UV
,136 ,079 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
EKSTRAK BUNGA BROKOLI 1% 3 20,67 20,67 EKSTRAK BUNGA BROKOLI 3% 3 27,00 Sig.
1 BLANKO 3 15,33
2
a
Banyaknya noda (spot)
8 Sebelum Penyinaran dengan UV Tukey HSD
8 P.Minggu IV Between Groups 180,667 2 90,333 3,535 ,097 Within Groups 153,333 6 25,556 Total 334,000
8 P. Minggu III Between Groups 364,667 2 182,333 40,024 ,000 Within Groups 27,333 6 4,556 Total 392,000
8 P. MingguII Between Groups 466,667 2 233,333 18,103 ,003 Within Groups 77,333 6 12,889 Total 544,000
8 P. Minggu I Between Groups 427,556 2 213,778 62,065 ,000 Within Groups 20,667 6 3,444 Total 448,222
8 Setelah UV Between Groups 46,889 2 23,444 ,238 ,795 Within Groups 591,333 6 98,556 Total 638,222
Within Groups 49,333 6 8,222 Total 254,000
Mean Square F Sig. SebelumUV Between Groups 204,667 2 102,333 12,446 ,007
Sum of Squares df
ANOVA
,817 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
Lampiran 12 (lanjutan) Pemulihan minggu I
Pemulihan minggu III
3 26,33 Sig.
1 EKSTRAK BUNGA BROKOLI 3% 3 16,67 EKSTRAK BUNGA BROKOLI 1% 3 17,00 BLANKO
FORMULA N Subset for alpha = .05
a
Tukey HSD
Pemulihan minggu IV
,980 1,000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
1 EKSTRAK BUNGA BROKOLI 1% 3 22,00 EKSTRAK BUNGA BROKOLI 3% 3 22,33 BLANKO 3 35,67 Sig.
2
FORMULA N Subset for alpha = .05
a
Tukey HSD
,528 1,000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
Tukey HSD
3 42,00 Sig.
1 EKSTRAK BUNGA BROKOLI 3% 3 25,33 EKSTRAK BUNGA BROKOLI 1% 3 28,67 BLANKO
2
FORMULA N Subset for alpha = .05
a
Tukey HSD
Pemulihan minggu II
,150 1,000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
3 47,00 Sig.
1 EKSTRAK BUNGA BROKOLI 3% 3 31,00 EKSTRAK BUNGA BROKOLI 1% 3 34,33 BLANKO
2
FORMULA N Subset for alpha = .05
a
,125 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
Lampiran 12 (lanjutan) 5.
FORMULA N Subset for alpha = .05
1 BLANKO 3 48,67 EKSTRAK BUNGA BROKOLI 1% 3 48,67 EKSTRAK BUNGA BROKOLI 3% 3 62,67 Sig.
1
FORMULA N Subset for alpha = .05
a
Tukey HSD
Setelah Penyinaran dengan UV
,174 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
1 BLANKO 3 5,00 EKSTRAK BUNGA BROKOLI 1% 3 5,33 EKSTRAK BUNGA BROKOLI 3% 3 8,00 Sig.
8 Sebelum Penyinaran dengan UV Tukey HSD
Keriput (wrinkle)
8 P.Minggu IV Between Groups 822,889 2 411,444 13,921 ,006 Within Groups 177,333 6 29,556 Total 1000,222
8 P. Minggu III Between Groups 380,667 2 190,333 7,167 ,026 Within Groups 159,333 6 26,556 Total 540,000
8 P. MingguII Between Groups 250,889 2 125,444 4,498 ,064 Within Groups 167,333 6 27,889 Total 418,222
8 P. Minggu I Between Groups 294,000 2 147,000 4,594 ,062 Within Groups 192,000 6 32,000 Total 486,000
8 Setelah UV Between Groups 392,000 2 196,000 2,841 ,136 Within Groups 414,000 6 69,000 Total 806,000
Within Groups 18,667 6 3,111 Total 34,889
Mean Square F Sig. SebelumUV Between Groups 16,222 2 8,111 2,607 ,153
Sum of Squares df
ANOVA
,178 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
Lampiran 12 (lanjutan) Pemulihan minggu I
a
1 EKSTRAK BUNGA BROKOLI 3% 3 6,33 EKSTRAK BUNGA BROKOLI 1% 3 14,00 BLANKO 3 29,33 Sig.
2
Tukey HSD FORMULA N Subset for alpha = .05
Pemulihan minggu IV
,461 ,108 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
1 EKSTRAK BUNGA BROKOLI 3% 3 14,00 EKSTRAK BUNGA BROKOLI 1% 3 19,33 19,33 BLANKO 3 29,67 Sig.
2
FORMULA N Subset for alpha = .05
Tukey HSD
Tukey HSD FORMULA N Subset for alpha = .05
Pemulihan minggu III
,079 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
1 EKSTRAK BUNGA BROKOLI 1% 3 22,33 EKSTRAK BUNGA BROKOLI 3% 3 23,33 BLANKO 3 34,00 Sig.
FORMULA N Subset for alpha = .05
a
Tukey HSD
Pemulihan minggu II
,052 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.
1 EKSTRAK BUNGA BROKOLI 1% 3 26,67 EKSTRAK BUNGA BROKOLI 3% 3 33,67 BLANKO 3 40,67 Sig.
,271 1,000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 3,000.