Pemberian Beberapa Jenis Antioksidan terhadap Peningkatan Ketahanan Salinitas pada Turunan F4 Kedelai berdasarkan Aktivitas Enzim Peroksidase (POD) dan Superoksida Dismutase (SOD)

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ABSTRAK

Lailasari Hutabarat, Pemberian Beberapa Jenis Antioksidan terhadap
Peningkatan Ketahanan Salinitas pada Turunan F4 Kedelai Berdasarkan Aktivitas
Enzin Peroksidase (POD) dan Superoksida Dismutase (SOD). Dibimbing
oleh Dr. Diana Sofia Hanfiah, SP., MP. dan Prof. Dr. Ir. Rosmayati, MS.
Peningkatan produksi kedelai dapat dilakukan dengan memanfaatan lahan
marginal seperti lahan salin melalui pemanfaatan genotipe hasil persilangan antara
toleran salin dengan berproduksi tinggi dan pemberian beberapa antioksidan yaitu
asam askorbat, asam salisilat, dan α-tokoferol untuk melindungi sel-sel tanaman dari
efek kondisi stres garam. Penelitian dilaksanakan di rumah plastik di lahan percobaan
Fakultas Pertanian Universitas Sumatera Utara, analisis karakter fisiologi dan biokimia
tanaman di Laboratorium Kultur Jaringan Tanaman USU, Balai Penelitian Sungei
Putih, Laboratorium Sentral USU bulan Februari sampai Agustus 2016. Penelitian
menggunakan Rancangan Acak Kelompok Satu Faktor yaitu perlakuan antioksidan
dan perlakuan diulang dua kali.
Hasil analisis data menunjukkan bahwa perlakuan antioksidan tidak
memberikan pengaruh yang nyata terhadap peningkatan ketahanan salinitas pada
turunan F4 kedelai. Secara deskriptif perlakuan asam askorbat 750 ppm memberikan

rataan tertinggi terhadap klorofil daun dan bobot biji per tanaman, perlakuan asam
salisilat 250 tertinggi pada enzim SOD, dan perlakuan asam salisilat 500 ppm tertinggi
pada enzim POD.
Kata kunci : antioksidan, salinitas, POD, SOD, kedelai

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Universitas Sumatera Utara

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ABSTRACT

Lailasari Hutabarat, Giving various type of antioxidant to Increase Resilience
Salinity on Enzyme Activity Based F4 Soybean peroxidase (POD) and superoxide
dismutase (SOD). Supervisied by Dr. Diana Sofia Hanfiah, SP., MP. and
Prof. Dr. Ir. Rosmayati, MS.
The production of soybean can be increased through a marginal land use such
as saline land, using genotype as the result of crossing the high-production tolerant
saline and giving various of antioxidant, such as ascorbic acid, salicylic acid, and
alpha-tocopherol to protect the plant cells from the effect salt-stress. This research

carried out in the plastic house in experimental field of Faculty of Agriculture
University of North Sumatera, physiology and biochemistry analysis in Plant Tissue
Culture Laboratory University of North Sumatera, Sungei Putih Research Center and
Central Laboratory of University of North Sumatera on February – August 2016. And
used factorial Randomized Block Design with one factor were antioxidant and two
replications.
The results showed that antioxidants not significantly effect to the increase of
tolerance salinity in the F4 generation of soybean. Descriptively, 750 ppm of ascorbic
acid treatment has given the highest average to chlorophyll analysis and weight of
seeds per sample, the highest treatment of 250 ppm of salicylic acid was to SOD
enzyme, and the highest treatment of 500 ppm of salicylic acid was to POD enzyme.
Keywords : antioxidant, salinity, POD, SOD, soybean

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Universitas Sumatera Utara