Gambaran Imunoekspresi p53 Mutan pada Karsinoma Sel Skuamosa Sinonasal
Abstrak
Pendahuluan : Karsinoma sel skuamosa sinonasal merupakan tumor ganas
yang terdapat pada kavum nasi dan sinus paranasal yang ditegakkan
berdasarkan hasil pemeriksaan histopatologi. Salah satu gen penekan tumor
(tumor suppressor genes) yaitu protein p53 yang berfungsi untuk menghambat
siklus sel, apoptosis, diferensiasi, penuaan sel dan menghambat angiogenesis.
Tujuan : Untuk mengetahui ekspresi p53 mutan pada karsinoma sel skuamosa
sinonasal.
Metode : Penelitian observasional yang dilakukan di RSUP. H. Adam
Malik Medan pada laboratorium Patologi Anatomi dengan pemeriksaan
immunohistokimia pada blok parafin dan data sampel penelitian
sebanyak 30 sampel.
Hasil Penelitian : Penderita karsinoma sel skuamosa sinonasal (SCC) paling
banyak ditemukan pada kelompok umur 41-60 tahun (63,3%), laki-laki (63,3%),
dan tipe non keratinizing squamous cell carcinoma 21 jaringan (70%), stadium
klinis yang terbanyak dijumpai pada stadium IV dengan 11 jaringan (36,7%).
Kesimpulan: Ekspresi protein p53 mutan yang terbanyak ditemukan
pada tipe non keratinizaing squamous cell carcinoma. Tidak terdapat
hubungan yang bermakna antara jenis histopatologi, stadium tumor
terhadap ekspresi protein p53 mutan dengan nilai p=0,440 (p>0,05).
Kata kunci : karsinoma sel skuamosa sinonasal, protein p53 mutan
v
Universitas Sumatera Utara
Abstract
Introduction : Squamous cell carcinoma of sinonasal (SCC) is a
malignant tumor in the nasal cavity and paranasal sinuses that was
established based on the results of histopathologic examination. p53
protein is one of the tumor suppressor genes that plays role in apoptosis,
differentiation, cellular aging, inhibiting the cell cycle and angiogenesis.
Aim: To determine the expression of p53 mutant in squamous cell
carcinoma of sinonasal
Method : The observational study was done in Anatomic Pathology Laboratory
of H. Adam Malik Medan General Hospital, by examining 30 paraffin blocks with
immunohistochemical stainning.
Result : Patients with SCC of the sinonasal was found higher in age of 4160(63,3%), male (63,3%). Non keratinizing squamous cell carcinoma type was
found in 21 samples (70%) and stage IV tumor in 11 sample (36,7%).
Conclusion : The expression of p53 protein mutant were observed mostly in
non keratinizing type SCC of the sinonasal. No significant correlation between
histopathologic type, tumor stadium and the expression of p53 protein mutant. p
value=0,440 (p>0,05).
Keyword : Squamous cell carcinoma of sinonasal, protein p53 mutant
vi
Universitas Sumatera Utara
Pendahuluan : Karsinoma sel skuamosa sinonasal merupakan tumor ganas
yang terdapat pada kavum nasi dan sinus paranasal yang ditegakkan
berdasarkan hasil pemeriksaan histopatologi. Salah satu gen penekan tumor
(tumor suppressor genes) yaitu protein p53 yang berfungsi untuk menghambat
siklus sel, apoptosis, diferensiasi, penuaan sel dan menghambat angiogenesis.
Tujuan : Untuk mengetahui ekspresi p53 mutan pada karsinoma sel skuamosa
sinonasal.
Metode : Penelitian observasional yang dilakukan di RSUP. H. Adam
Malik Medan pada laboratorium Patologi Anatomi dengan pemeriksaan
immunohistokimia pada blok parafin dan data sampel penelitian
sebanyak 30 sampel.
Hasil Penelitian : Penderita karsinoma sel skuamosa sinonasal (SCC) paling
banyak ditemukan pada kelompok umur 41-60 tahun (63,3%), laki-laki (63,3%),
dan tipe non keratinizing squamous cell carcinoma 21 jaringan (70%), stadium
klinis yang terbanyak dijumpai pada stadium IV dengan 11 jaringan (36,7%).
Kesimpulan: Ekspresi protein p53 mutan yang terbanyak ditemukan
pada tipe non keratinizaing squamous cell carcinoma. Tidak terdapat
hubungan yang bermakna antara jenis histopatologi, stadium tumor
terhadap ekspresi protein p53 mutan dengan nilai p=0,440 (p>0,05).
Kata kunci : karsinoma sel skuamosa sinonasal, protein p53 mutan
v
Universitas Sumatera Utara
Abstract
Introduction : Squamous cell carcinoma of sinonasal (SCC) is a
malignant tumor in the nasal cavity and paranasal sinuses that was
established based on the results of histopathologic examination. p53
protein is one of the tumor suppressor genes that plays role in apoptosis,
differentiation, cellular aging, inhibiting the cell cycle and angiogenesis.
Aim: To determine the expression of p53 mutant in squamous cell
carcinoma of sinonasal
Method : The observational study was done in Anatomic Pathology Laboratory
of H. Adam Malik Medan General Hospital, by examining 30 paraffin blocks with
immunohistochemical stainning.
Result : Patients with SCC of the sinonasal was found higher in age of 4160(63,3%), male (63,3%). Non keratinizing squamous cell carcinoma type was
found in 21 samples (70%) and stage IV tumor in 11 sample (36,7%).
Conclusion : The expression of p53 protein mutant were observed mostly in
non keratinizing type SCC of the sinonasal. No significant correlation between
histopathologic type, tumor stadium and the expression of p53 protein mutant. p
value=0,440 (p>0,05).
Keyword : Squamous cell carcinoma of sinonasal, protein p53 mutant
vi
Universitas Sumatera Utara