Efek Relaksasi Ekstrak Etanol Daun Pugun Tanoh (Curanga Fel-Terrae (Lour) Merr.) Terhadap Otot Polos Trakea Marmut Terisolasi Dan Pengaruhnya Pada Fosfodiesterase
Lampiran 1. Hasil identifikasi tanaman
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Lampiran 2. Karakteristik daun pugun tanoh
Makroskopik daun pugun tanoh
Serbuk simplisia daun pugun tanoh
Universitas Sumatera Utara
a
b
c
d
e
f
g
h
Mikroskopik serbuk simplisia daun pugun tanoh
Keterangan:
a. Sel epidermis
b. tulang daun
c. Trikoma
d. Kristal kalsium oksalat bentuk prisma
e. Berkas pembuluh angkut bentuk spiral
f. Stomata tipe diasitik
g. Stomata tipe anomositik
h. Sel tetangga
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Lampiran 3. Hasil karakterisasi Simplisia
1. Penetapan Kadar air
% Kadar air simplisia =
No.
1.
2.
3.
Berat sampel (g)
5,043
5,032
5,021
% Kadar air =
volume air (ml )
berat sampel (g)
Volume awal (ml)
1,80
2,10
2,40
volume akhir −volume awal
berat sampel
1. % Kadar air =
2. % Kadar air =
3. % Kadar air =
x 100%
2,10 − 1,80
5,043
2,40 − 2,10
5,032
2,70 − 2,40
5,021
% rata-rata kadar air =
Volume akhir (ml)
2,10
2,40
2,70
x 100%
x 100% = 5,95%
x 100% = 5,96%
x 100% = 5,97%
5,95% + 5,96% + 5,97%
3
= 5,96%
Universitas Sumatera Utara
2. Penetapan Kadar Sari Larut Air
% Kadar sari larut dalam air =
No.
1.
2.
3.
berat sari (g)
berat sampel (g)
Berat sampel (g)
5,032
5,035
5,025
1. % Kadar sari larut dalam air =
2. % Kadar sari larut dalam air =
3. % Kadar sari larut dalam air =
x
100
20
x 100%
Berat sari (g)
0,177
0,158
0,159
0,177
x
5,032
0,158
x
5,035
0,159
5,025
% rata-rata kadar sari larut dalam air =
x
100
20
100
20
100
20
x 100% = 17,58%
x 100% = 15,69%
x 100% = 15,82%
17,58% + 15,69% + 15,82%
3
= 16,36%
3. Penetapan Kadar Sari Larut Etanol
% Kadar sari larut dalam etanol =
No.
1.
2.
3.
Berat sampel (g)
5,077
5,044
5,035
1. % Kadar sari larut dalam air =
2. % Kadar sari larut dalam air =
3. % Kadar sari larut dalam air =
berat sari (g)
berat sampel (g)
x
100
20
x 100%
Berat sari (g)
0,160
0,124
0,130
0,160
5,077
0,124
5,044
0,130
5,035
% rata-rata kadar sari larut dalam air =
x
x
x
100
20
100
20
100
20
x 100% = 15,75%
x 100% = 12,29%
x 100% = 12,91%
15,75% + 12,29% + 12,91%
3
= 13,65%
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4. Penetapan Kadar Abu Total
berat abu (g)
% Kadar abu total =
No.
1.
2.
3.
berat sampel (g)
Berat sampel (g)
2,017
2,034
2,022
1. % Kadar abu total =
2. % Kadar abu total =
3. % Kadar abu total =
x 100%
Berat abu (g)
0,171
0,176
0,173
0,171
2,017
0,176
2,034
0,173
2,022
x 100% = 8,48%
x 100% = 8,65%
x 100% = 8,56%
% rata-rata kadar abu total =
8,48% +8,65%+8,56%
3
= 8,56%
5. Penetapan Kadar Abu Tidak Larut Asam
% Kadar abu total =
No.
1.
2.
3.
Berat sampel (g)
2,017
2,034
2,022
1. % Kadar abu tidak larut asam =
2. % Kadar abu tidak larut asam =
3. % Kadar abu tidak larut asam =
berat abu (g)
berat sampel (g)
x 100%
Berat abu (g)
0,021
0,021
0,019
0,021
2,017
0,021
2,034
0,019
2,022
% rata-rata kadar abu tidak larut asam =
x 100% = 1,04%
x 100% = 1,03%
x 100% = 0,94%
1,04%+1,03%+0,94%
3
= 1,00%
Universitas Sumatera Utara
Lampiran 4. Alat organ bath
Bejana organ
Monitor komputer dan seperangkat alat Power Lab
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Lampiran 5. Pola kenaikan kontraksi otot polos trakea marmut terisolasi setelah
diinduksi asetilkolin konsentrasi bertingkat
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Lampiran 6. Pola penurunan kontraksi otot polos trakea marmut terisolasi setelah
pemberian EEDPT
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Lampiran 7. Perhitungan effective concentration (EC80) asetilkolin terhadap otot
polos trakea
Log
konsentrasi
Ach
-8.00
-7.52
-7.00
-6.52
-6.00
-5.52
-5.00
-4.52
-4.00
-3.52
-3.00
-2.52
Keterangan:
% Kontraksi Trakea
I
II
III
3.6364
1.5504
9.0909
3.1008
10.9091
5.0387
10.9091
5.0387
9.0909
5.4263
8.1818
7.7519
13.6364 10.0775
22.7273 24.0310
40.0000 36.4341
55.4545 51.9380
79.0909 71.3178
100.0000 100.0000
Mean
IV
2.7586
1.6260
2.3929
3.4483
2.4390
4.5198
4.1379
3.2520
5.8344
4.8276
4.0650
6.2101
5.5172
4.8780
6.2281
5.5172
5.6910
6.7855
6.8965
7.3171
9.4819
11.0345
7.3171 16.2775
25.8621 16.2602 29.6391
40.6896 38.2114 46.5734
66.2069 66.6667 70.8206
100.0000 100.0000 100.0000
SEM
0.4456
1.3757
1.5477
1.4134
0.8628
0.6159
1.3901
3.7372
4.8082
3.7612
2.6732
0.0000
80 – �1
Log EC80 = �
× (�2 − �1 )� + �1
�2 – �1
X1
: Log. konsentrasi dengan respon tepat di bawah 80%
X2
: Log. konsentrasi dengan rspon tepat di atas 80%
Y1
: % respon tepat di bawah 80%
Y2
: % respon tepat di atas 80%
80 –79,0909
× (−2.5 − (−3))� + − 3
1. Log EC80 = �
100 – 79,0909
= -2,9791
EC80 = 1,05x10-3 M
80 –71,3178
2. Log EC80 = �
× (−2.5 − (−3))� + − 3
100 – 71,3178
= -2,8547
EC80 = 1,40x10-3 M
Universitas Sumatera Utara
80 –66,2069
× (−2.5 − (−3))� + − 3
3. Log EC80 = �
100 – 66,2069
= -2,8041
EC80 = 1,57x10-3 M
80 –66,6667
4. Log EC80 = �
× (−2.5 − (−3))� + − 3
100 – 66,6667
= -2,808
EC80 = 1,56x10-3 M
EC80 rata-rata =
1,05+1,40+1,57+1,56
4
x 10-3 M = 1,39x10-3 M
Universitas Sumatera Utara
Lampiran 8. Analisis uji-t independen nilai % relaksasi EEDPT terhadap
kontraksi otot polos trakea oleh pemberian asetilkolin 10-3 M
dengan inkubasi awal menggunakan teofilin 10-4 M selama 20
menit dengan % relaksasi EEDPT terhadap kontraksi otot polos
trakea oleh pemberian asetilkolin 10-3 M tanpa inkubasi sebagai
kontrol
Tests of Normality
Kolmogorov-Smirnov(a)
Statistic
df
Shapiro-Wilk
Sig.
Statistic
df
Sig.
EEPT 1mg
.173
10
.200(*)
.940
10
.556
EEPT 2 mg
.158
10
.200(*)
.917
10
.330
EEPT 3 mg
.120
10
.200(*)
.966
10
.847
EEPT 4 mg
.156
10
.200(*)
.925
10
.402
EEPT 5 mg
.218
10
.196
.909
10
.274
EEPT 6 mg
.167
10
.200(*)
.927
10
.414
EEPT 7 mg
.226
10
.158
.859
10
.075
EEPT 8 mg
.233
10
.134
.896
10
.197
* This is a lower bound of the true significance.
a Lilliefors Significance Correction
T-Test
Group Statistics
EEPT 1mg
EEPT 2 mg
EEPT 3 mg
EEPT 4 mg
EEPT 5 mg
EEPT 6 mg
EEPT 7 mg
EEPT 8 mg
perlakuan
Ach+EEPT
N
Mean
Std.
Deviation
Std. Error
Mean
5
6.531060
3.6038342
1.6116836
TPH+Ach+EEPT
5
6.098880
2.8385225
1.2694258
Ach+EEPT
5
15.660680
9.9471030
4.4484797
TPH+Ach+EEPT
5
15.054960
5.1239574
2.2915034
Ach+EEPT
5
26.868900
9.1365506
4.0859896
TPH+Ach+EEPT
5
28.479840
7.4536696
3.3333824
Ach+EEPT
5
38.245280
11.5233604
5.1534035
TPH+Ach+EEPT
5
47.325980
11.8075767
5.2805088
Ach+EEPT
5
54.075720
10.8513720
4.8528811
TPH+Ach+EEPT
5
69.505460
13.3574299
5.9736243
Ach+EEPT
5
72.163680
11.3417506
5.0721851
TPH+Ach+EEPT
5
91.465180
19.3137252
8.6373605
Ach+EEPT
5
93.339120
5.7756517
2.5829500
TPH+Ach+EEPT
5
105.970680
14.1201446
6.3147206
Ach+EEPT
5
123.514800
9.8573786
4.4083537
TPH+Ach+EEPT
5
130.347980
19.1238187
8.5524317
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Independent Samples Test
Levene's Test
for Equality of
Variances
F
EEPT
1mg
EEPT
2 mg
EEPT
3 mg
EEPT
4 mg
EEPT
5 mg
EEPT
6 mg
EEPT
7 mg
EEPT
8 mg
Equal
variances
as sumed
Equal
variances
not
as sumed
Equal
variances
as sumed
Equal
variances
not
as sumed
Equal
variances
as sumed
Equal
variances
not
as sumed
Equal
variances
as sumed
Equal
variances
not
as sumed
Equal
variances
as sumed
Equal
variances
not
as sumed
Equal
variances
as sumed
Equal
variances
not
as sumed
Equal
variances
as sumed
Equal
variances
not
as sumed
Equal
variances
as sumed
Equal
variances
not
as sumed
.266
1.337
.308
.017
1.163
.768
.815
1.172
Sig.
.620
.281
.594
.901
.312
.406
.393
.311
t-test for Equality of Means
t
df
Sig.
(2-tail
ed)
Mean
Difference
Std.
Error
Differenc
e
95% Confidence
Interval of the
Difference
Lower
Upper
.211
8
.838
.4321800
2.05158
-4.29876
5.16312
.211
7.584
.839
.4321800
2.05158
-4.34435
5.20871
.121
8
.907
.6057200
5.00399
-10.9335
12.1450
.121
5.983
.908
.6057200
5.00399
-11.6470
12.8584
-.305
8
.768
-1.610940
5.27321
-13.7710
10.5491
-.305
7.690
.768
-1.610940
5.27321
-13.8569
10.6350
-1.231
8
.253
-9.080700
7.37844
-26.0954
7.93401
-1.231
7.995
.253
-9.080700
7.37844
-26.0972
7.93577
-2.005
8
.080
-15.42974
7.69640
-33.1777
2.31820
-2.005
7.678
.081
-15.42974
7.69640
-33.3081
2.44867
-1.927
8
.090
-19.30150
10.0165
-42.3997
3.79668
-1.927
6.466
.099
-19.30150
10.0165
-43.3896
4.78656
-1.851
8
.101
-12.63156
6.82256
-28.3644
3.10129
-1.851
5.302
.120
-12.63156
6.82256
-29.8732
4.61011
-.710
8
.498
-6.833180
9.62173
-29.0209
15.3546
-.710
5.985
.504
-6.833180
9.62173
-30.3907
16.7243
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Lampiran 9. Uji korelasi regresi antara dosis EEDPT dengan besarnya efek
relaksasi otot polos trakea
Correlations
De scri ptive Statistics
Mean
dosis E EP T
4.50
% relaksas i 53.799963
St d. Deviat ion
2.449
40.3856117
N
8
8
Correlations
dosis EEPT
% relaksas i
Pearson Correlation
Sig. (2-tailed)
N
Pearson Correlation
Sig. (2-tailed)
N
dosis EEPT
1
% relaksas i
.981**
.000
8
8
1
.981**
.000
8
8
**. Correlation is s ignificant at the 0.01 level (2-tailed).
Regression
De scri ptive Statistics
Mean
% relaksas i 53.799963
dosis E EP T
4.50
St d. Deviat ion
40.3856117
2.449
N
8
8
Correl ations
Pearson Correlation
Sig. (1-tailed)
N
% relaksas i
dosis E EP T
% relaksas i
dosis E EP T
% relaksas i
dosis E EP T
% relaksas i
1.000
.981
.
.000
8
8
dosis E EP T
.981
1.000
.000
.
8
8
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b
Va riables Entere d/Re moved
Model
1
Variables
Entered
dosis a
EE PT
Variables
Removed
Method
.
Enter
a. All reques ted variables ent ered.
b. Dependent Variable: % relaksasi
Model Summary
Model
1
R
R Square
a
.981
.963
Adjusted
R Square
.957
Std. Error of
the Estimate
8.4039254
a. Predictors: (Constant), dosis EEPT
Coeffi cientsa
Model
1
(Const ant)
dosis E EP T
Unstandardized
Coeffic ients
B
St d. E rror
-19.003
6.548
16.178
1.297
St andardiz ed
Coeffic ients
Beta
.981
t
-2. 902
12.476
Sig.
.027
.000
a. Dependent Variable: % relak sas i
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Lampiran 2. Karakteristik daun pugun tanoh
Makroskopik daun pugun tanoh
Serbuk simplisia daun pugun tanoh
Universitas Sumatera Utara
a
b
c
d
e
f
g
h
Mikroskopik serbuk simplisia daun pugun tanoh
Keterangan:
a. Sel epidermis
b. tulang daun
c. Trikoma
d. Kristal kalsium oksalat bentuk prisma
e. Berkas pembuluh angkut bentuk spiral
f. Stomata tipe diasitik
g. Stomata tipe anomositik
h. Sel tetangga
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Lampiran 3. Hasil karakterisasi Simplisia
1. Penetapan Kadar air
% Kadar air simplisia =
No.
1.
2.
3.
Berat sampel (g)
5,043
5,032
5,021
% Kadar air =
volume air (ml )
berat sampel (g)
Volume awal (ml)
1,80
2,10
2,40
volume akhir −volume awal
berat sampel
1. % Kadar air =
2. % Kadar air =
3. % Kadar air =
x 100%
2,10 − 1,80
5,043
2,40 − 2,10
5,032
2,70 − 2,40
5,021
% rata-rata kadar air =
Volume akhir (ml)
2,10
2,40
2,70
x 100%
x 100% = 5,95%
x 100% = 5,96%
x 100% = 5,97%
5,95% + 5,96% + 5,97%
3
= 5,96%
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2. Penetapan Kadar Sari Larut Air
% Kadar sari larut dalam air =
No.
1.
2.
3.
berat sari (g)
berat sampel (g)
Berat sampel (g)
5,032
5,035
5,025
1. % Kadar sari larut dalam air =
2. % Kadar sari larut dalam air =
3. % Kadar sari larut dalam air =
x
100
20
x 100%
Berat sari (g)
0,177
0,158
0,159
0,177
x
5,032
0,158
x
5,035
0,159
5,025
% rata-rata kadar sari larut dalam air =
x
100
20
100
20
100
20
x 100% = 17,58%
x 100% = 15,69%
x 100% = 15,82%
17,58% + 15,69% + 15,82%
3
= 16,36%
3. Penetapan Kadar Sari Larut Etanol
% Kadar sari larut dalam etanol =
No.
1.
2.
3.
Berat sampel (g)
5,077
5,044
5,035
1. % Kadar sari larut dalam air =
2. % Kadar sari larut dalam air =
3. % Kadar sari larut dalam air =
berat sari (g)
berat sampel (g)
x
100
20
x 100%
Berat sari (g)
0,160
0,124
0,130
0,160
5,077
0,124
5,044
0,130
5,035
% rata-rata kadar sari larut dalam air =
x
x
x
100
20
100
20
100
20
x 100% = 15,75%
x 100% = 12,29%
x 100% = 12,91%
15,75% + 12,29% + 12,91%
3
= 13,65%
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4. Penetapan Kadar Abu Total
berat abu (g)
% Kadar abu total =
No.
1.
2.
3.
berat sampel (g)
Berat sampel (g)
2,017
2,034
2,022
1. % Kadar abu total =
2. % Kadar abu total =
3. % Kadar abu total =
x 100%
Berat abu (g)
0,171
0,176
0,173
0,171
2,017
0,176
2,034
0,173
2,022
x 100% = 8,48%
x 100% = 8,65%
x 100% = 8,56%
% rata-rata kadar abu total =
8,48% +8,65%+8,56%
3
= 8,56%
5. Penetapan Kadar Abu Tidak Larut Asam
% Kadar abu total =
No.
1.
2.
3.
Berat sampel (g)
2,017
2,034
2,022
1. % Kadar abu tidak larut asam =
2. % Kadar abu tidak larut asam =
3. % Kadar abu tidak larut asam =
berat abu (g)
berat sampel (g)
x 100%
Berat abu (g)
0,021
0,021
0,019
0,021
2,017
0,021
2,034
0,019
2,022
% rata-rata kadar abu tidak larut asam =
x 100% = 1,04%
x 100% = 1,03%
x 100% = 0,94%
1,04%+1,03%+0,94%
3
= 1,00%
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Lampiran 4. Alat organ bath
Bejana organ
Monitor komputer dan seperangkat alat Power Lab
Universitas Sumatera Utara
Lampiran 5. Pola kenaikan kontraksi otot polos trakea marmut terisolasi setelah
diinduksi asetilkolin konsentrasi bertingkat
Universitas Sumatera Utara
Lampiran 6. Pola penurunan kontraksi otot polos trakea marmut terisolasi setelah
pemberian EEDPT
Universitas Sumatera Utara
Lampiran 7. Perhitungan effective concentration (EC80) asetilkolin terhadap otot
polos trakea
Log
konsentrasi
Ach
-8.00
-7.52
-7.00
-6.52
-6.00
-5.52
-5.00
-4.52
-4.00
-3.52
-3.00
-2.52
Keterangan:
% Kontraksi Trakea
I
II
III
3.6364
1.5504
9.0909
3.1008
10.9091
5.0387
10.9091
5.0387
9.0909
5.4263
8.1818
7.7519
13.6364 10.0775
22.7273 24.0310
40.0000 36.4341
55.4545 51.9380
79.0909 71.3178
100.0000 100.0000
Mean
IV
2.7586
1.6260
2.3929
3.4483
2.4390
4.5198
4.1379
3.2520
5.8344
4.8276
4.0650
6.2101
5.5172
4.8780
6.2281
5.5172
5.6910
6.7855
6.8965
7.3171
9.4819
11.0345
7.3171 16.2775
25.8621 16.2602 29.6391
40.6896 38.2114 46.5734
66.2069 66.6667 70.8206
100.0000 100.0000 100.0000
SEM
0.4456
1.3757
1.5477
1.4134
0.8628
0.6159
1.3901
3.7372
4.8082
3.7612
2.6732
0.0000
80 – �1
Log EC80 = �
× (�2 − �1 )� + �1
�2 – �1
X1
: Log. konsentrasi dengan respon tepat di bawah 80%
X2
: Log. konsentrasi dengan rspon tepat di atas 80%
Y1
: % respon tepat di bawah 80%
Y2
: % respon tepat di atas 80%
80 –79,0909
× (−2.5 − (−3))� + − 3
1. Log EC80 = �
100 – 79,0909
= -2,9791
EC80 = 1,05x10-3 M
80 –71,3178
2. Log EC80 = �
× (−2.5 − (−3))� + − 3
100 – 71,3178
= -2,8547
EC80 = 1,40x10-3 M
Universitas Sumatera Utara
80 –66,2069
× (−2.5 − (−3))� + − 3
3. Log EC80 = �
100 – 66,2069
= -2,8041
EC80 = 1,57x10-3 M
80 –66,6667
4. Log EC80 = �
× (−2.5 − (−3))� + − 3
100 – 66,6667
= -2,808
EC80 = 1,56x10-3 M
EC80 rata-rata =
1,05+1,40+1,57+1,56
4
x 10-3 M = 1,39x10-3 M
Universitas Sumatera Utara
Lampiran 8. Analisis uji-t independen nilai % relaksasi EEDPT terhadap
kontraksi otot polos trakea oleh pemberian asetilkolin 10-3 M
dengan inkubasi awal menggunakan teofilin 10-4 M selama 20
menit dengan % relaksasi EEDPT terhadap kontraksi otot polos
trakea oleh pemberian asetilkolin 10-3 M tanpa inkubasi sebagai
kontrol
Tests of Normality
Kolmogorov-Smirnov(a)
Statistic
df
Shapiro-Wilk
Sig.
Statistic
df
Sig.
EEPT 1mg
.173
10
.200(*)
.940
10
.556
EEPT 2 mg
.158
10
.200(*)
.917
10
.330
EEPT 3 mg
.120
10
.200(*)
.966
10
.847
EEPT 4 mg
.156
10
.200(*)
.925
10
.402
EEPT 5 mg
.218
10
.196
.909
10
.274
EEPT 6 mg
.167
10
.200(*)
.927
10
.414
EEPT 7 mg
.226
10
.158
.859
10
.075
EEPT 8 mg
.233
10
.134
.896
10
.197
* This is a lower bound of the true significance.
a Lilliefors Significance Correction
T-Test
Group Statistics
EEPT 1mg
EEPT 2 mg
EEPT 3 mg
EEPT 4 mg
EEPT 5 mg
EEPT 6 mg
EEPT 7 mg
EEPT 8 mg
perlakuan
Ach+EEPT
N
Mean
Std.
Deviation
Std. Error
Mean
5
6.531060
3.6038342
1.6116836
TPH+Ach+EEPT
5
6.098880
2.8385225
1.2694258
Ach+EEPT
5
15.660680
9.9471030
4.4484797
TPH+Ach+EEPT
5
15.054960
5.1239574
2.2915034
Ach+EEPT
5
26.868900
9.1365506
4.0859896
TPH+Ach+EEPT
5
28.479840
7.4536696
3.3333824
Ach+EEPT
5
38.245280
11.5233604
5.1534035
TPH+Ach+EEPT
5
47.325980
11.8075767
5.2805088
Ach+EEPT
5
54.075720
10.8513720
4.8528811
TPH+Ach+EEPT
5
69.505460
13.3574299
5.9736243
Ach+EEPT
5
72.163680
11.3417506
5.0721851
TPH+Ach+EEPT
5
91.465180
19.3137252
8.6373605
Ach+EEPT
5
93.339120
5.7756517
2.5829500
TPH+Ach+EEPT
5
105.970680
14.1201446
6.3147206
Ach+EEPT
5
123.514800
9.8573786
4.4083537
TPH+Ach+EEPT
5
130.347980
19.1238187
8.5524317
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Independent Samples Test
Levene's Test
for Equality of
Variances
F
EEPT
1mg
EEPT
2 mg
EEPT
3 mg
EEPT
4 mg
EEPT
5 mg
EEPT
6 mg
EEPT
7 mg
EEPT
8 mg
Equal
variances
as sumed
Equal
variances
not
as sumed
Equal
variances
as sumed
Equal
variances
not
as sumed
Equal
variances
as sumed
Equal
variances
not
as sumed
Equal
variances
as sumed
Equal
variances
not
as sumed
Equal
variances
as sumed
Equal
variances
not
as sumed
Equal
variances
as sumed
Equal
variances
not
as sumed
Equal
variances
as sumed
Equal
variances
not
as sumed
Equal
variances
as sumed
Equal
variances
not
as sumed
.266
1.337
.308
.017
1.163
.768
.815
1.172
Sig.
.620
.281
.594
.901
.312
.406
.393
.311
t-test for Equality of Means
t
df
Sig.
(2-tail
ed)
Mean
Difference
Std.
Error
Differenc
e
95% Confidence
Interval of the
Difference
Lower
Upper
.211
8
.838
.4321800
2.05158
-4.29876
5.16312
.211
7.584
.839
.4321800
2.05158
-4.34435
5.20871
.121
8
.907
.6057200
5.00399
-10.9335
12.1450
.121
5.983
.908
.6057200
5.00399
-11.6470
12.8584
-.305
8
.768
-1.610940
5.27321
-13.7710
10.5491
-.305
7.690
.768
-1.610940
5.27321
-13.8569
10.6350
-1.231
8
.253
-9.080700
7.37844
-26.0954
7.93401
-1.231
7.995
.253
-9.080700
7.37844
-26.0972
7.93577
-2.005
8
.080
-15.42974
7.69640
-33.1777
2.31820
-2.005
7.678
.081
-15.42974
7.69640
-33.3081
2.44867
-1.927
8
.090
-19.30150
10.0165
-42.3997
3.79668
-1.927
6.466
.099
-19.30150
10.0165
-43.3896
4.78656
-1.851
8
.101
-12.63156
6.82256
-28.3644
3.10129
-1.851
5.302
.120
-12.63156
6.82256
-29.8732
4.61011
-.710
8
.498
-6.833180
9.62173
-29.0209
15.3546
-.710
5.985
.504
-6.833180
9.62173
-30.3907
16.7243
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Lampiran 9. Uji korelasi regresi antara dosis EEDPT dengan besarnya efek
relaksasi otot polos trakea
Correlations
De scri ptive Statistics
Mean
dosis E EP T
4.50
% relaksas i 53.799963
St d. Deviat ion
2.449
40.3856117
N
8
8
Correlations
dosis EEPT
% relaksas i
Pearson Correlation
Sig. (2-tailed)
N
Pearson Correlation
Sig. (2-tailed)
N
dosis EEPT
1
% relaksas i
.981**
.000
8
8
1
.981**
.000
8
8
**. Correlation is s ignificant at the 0.01 level (2-tailed).
Regression
De scri ptive Statistics
Mean
% relaksas i 53.799963
dosis E EP T
4.50
St d. Deviat ion
40.3856117
2.449
N
8
8
Correl ations
Pearson Correlation
Sig. (1-tailed)
N
% relaksas i
dosis E EP T
% relaksas i
dosis E EP T
% relaksas i
dosis E EP T
% relaksas i
1.000
.981
.
.000
8
8
dosis E EP T
.981
1.000
.000
.
8
8
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b
Va riables Entere d/Re moved
Model
1
Variables
Entered
dosis a
EE PT
Variables
Removed
Method
.
Enter
a. All reques ted variables ent ered.
b. Dependent Variable: % relaksasi
Model Summary
Model
1
R
R Square
a
.981
.963
Adjusted
R Square
.957
Std. Error of
the Estimate
8.4039254
a. Predictors: (Constant), dosis EEPT
Coeffi cientsa
Model
1
(Const ant)
dosis E EP T
Unstandardized
Coeffic ients
B
St d. E rror
-19.003
6.548
16.178
1.297
St andardiz ed
Coeffic ients
Beta
.981
t
-2. 902
12.476
Sig.
.027
.000
a. Dependent Variable: % relak sas i
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