Efek Relaksasi Ekstrak Etanol Daun Pugun Tanoh (Curanga fel-terrae (Lour.) Merr.) Terhadap Kontraksi Otot Polos Ileum Marmut (Cavia porcellus) Terisolasi Secara In Vitro

  Lampiran 1. Hasil identifikasi tumbuhan

  Lampiran 2. Gambar Tumbuhan Pugun Tanoh ( Curanga Fel-terrae (Lour.) Merr.)

  Lampiran 3. Karakteristik daun Pugun Tanoh Gambar Daun Pugun Tanoh

  

Lampiran 4. Hasil pemeriksaan mikroskopik serbuk simplisia daun Pugun Tanoh

  a b c e f d g

  Gambar Mikroskopik serbuk simplisia Keterangan: a. Trikoma

  b. Sel epidermis

  c. Kristal kalsium oksalat bentuk prisma

  d. Berkas pembuluh angkut bentuk spiral

  e. Stomata tipe diasitik

  f. Sel tetangga

  g. Stomata tipe anomositik

  Lampiran 5. Bagan Pembuatan, skrining fitokimia dan karakterisasi serbuk simplisia Daun Pugun Tanoh dicuci sampai bersih dikeringkan di lemari pengering o dengan suhu ± 40 C Simplisia dihaluskan Serbuk simplisia daun Pugun Tanoh

  Skrining Fitokimia: Karakterisasi Simplisia:

• Alkaloid - Mikroskopik - Flavonoid - Makroskopik - Saponin - Penetapan kadar:
• Tanin air

  sari yang larut dalam etanol

• Glikosida
• sari yang larut dalam air
• Antrakuinon abu total
• Steroid/Triterpenoida
• abu yang tidak larut dalam asam

  Lampiran 6. Bagan Pembuatan, skrining fitokimia dan karakterisasi ekstrak Serbuk simplisia daun Pugun Tanoh diperkolasi dengan cairan penyari etanol 96% Ekstrak Cair dipekatkan dengan rotary evaporator dikeringkan dengan freeze dryer

  Ekstrak Kering Skrining Fitokimia: Karakterisasi Ekstrak:

• Alkaloid - Penetapan kadar:
• Flavonoid air
• sari yang larut dalam etanol
• Saponin
• sari yang larut dalam air
• Tanin abu total
• Glikosida abu yang tidak larut dalam asam
• Antrakuinon - Steroid/Triterpenoida Ekstrak etanol daun Pugun Tanoh

  Lampiran 7. Bagan kerja pengukuran kontraksi ileum marmut terisolasi Pengujian kontraksi seri konsentrasi Asetilkolin terhadap otot polos ileum

  Pengujian efek relaksasi ekstrak etanol daun Pugun

  Tanoh pada kontraksi otot polos ileum melalui induksi

  Asetilkolin Pengujian efek relaksasi Atropin Sulfat pada kontraksi otot polos ileum melalui induksi Asetilkolin

  Pengujian mekanisme aksi terhadap efek relaksasi ekstrak etanol daun Pugun Tanoh pada otot polos ileum melalui penghambatan produksi PGE

  2 oleh Aspirin

  Marmut dislokasi tulang belakang kepala Preparasi organ Ekuilibrasi selama 45 menit

  (diganti larutan tirode setiap 15 menit) Tahapan pengujian

  Lampiran 8. Perhitungan kadar air simplisia daun Pugun Tanoh volume air (ml )

  % Kadar air simplisia = x 100% berat sampel (g)

  No. Berat sampel (g) Volume awal (ml) Volume akhir (ml) 1. 5,043 1,90 2,20 2. 5,032 2,20 2,50 3. 5,021 2,50 2,80 volume akhir − volume awal

  % Kadar air = x 100% berat sampel

  2,20 - 1,90

  1. Kadar air = = x 100% 5,95% 5,043

  50 2,20 - 2,

  2. Kadar air = x 100% = 5,96% 5,032 2,80 2,50 -

  =

  3. Kadar air = x 100% 5 ,97% 5,021 5,95% + 5,96% + 5,97%

  % Rata-rata kadar air = = 5,96%

  3

  Lampiran 9. Perhitungan kadar abu total simplisia daun Pugun Tanoh berat abu (g)

  % Kadar abu total = x 100% berat sampel (g)

  No. Berat sampel (g) Berat abu (g) 1. 2,0171 0,1709 2. 2,0342 0,1760 3. 2,0221 0,1732

  0,1709

  1. Kadar abu total = = x 100% 8,47% 2,0171

  0,1760

  2. Kadar abu total = x 100% = 8,65% 2,0342 0,1732

  3. Kadar abu total = = x 100% 8,57% 2,0221

  

8,47%+ 8,655+8,57%

% Rata-rata kadar abu total = = 8,56%

  

3

  Lampiran 10. Perhitungan kadar abu tidak larut asam simplisia daun Pugun Tanoh berat abu (g)

  % Kadar abu total = x 100% berat sampel (g)

  No. Berat sampel (g) Berat abu (g) 1. 2,0171 0,0210 2. 2,0342 0,0209 3. 2,0221 0,0192

  0,0210

  1. Kadar abu total = = x 100% 1,04% 2,0171

  0.0209 =

  2. Kadar abu total = x 100% 1,03% 2,0342 0,0192

  3. Kadar abu total = = x 100% 0,95% 2,0221

  

1,04%+1,03%+0,95%

% Rata-rata kadar abu total = = 1,01%

  

3

  Lampiran 11. Perhitungan kadar sari larut air simplisia daun Pugun Tanoh % Kadar sari larut dalam air = berat sari (g) berat sampel (g) x

  100

  20 x

  100% No. Berat sampel (g) Berat sari (g) 1. 5,032 0,177 2. 5,035 0,158 3. 5,025 0,159

  1. Kadar sari larut dalam air = 17,58% 100% x

  20

100

x 5,032

  0,177 =

  2. Kadar sari larut dalam air = 15,69% 100% x

  20

100

x 5,035

  0,158 =

  3. Kadar sari larut dalam air = 15,82% 100% x

  20

100

x 5,025

  0,159 = % Rata-rata kadar sari larut dalam air= % 36 ,

  16

  3 15,82% 15,69% 17,58% =

  3 12,91% 12,29% 15,75% =

  20 100 x

5,044

  13

  65 ,

  

0,130

= % Rata-rata kadar sari larut dalam etanol = %

  20 100 x

5,035

  3. Kadar sari larut dalam etanol = 12,91% 100% x

  

0,124

=

  2. Kadar sari larut dalam etanol = 12,29% 100% x

  Lampiran 12. Perhitungan kadar sari larut etanol simplisia daun Pugun Tanoh % Kadar sari larut dalam etanol =

berat sari (g)

berat sampel (g)

x

  

0,160

=

  20 100 x

5,077

  1. Kadar sari larut dalam etanol = 15,75% 100% x

  100% No. Berat sampel (g) Berat sari (g) 1. 5,077 0,160 2. 5,044 0,124 3. 5,035 0,130

  20 x

  100

  Lampiran 13. Perhitungan kadar air ekstrak etanol daun Pugun Tanoh volume air (ml )

  % Kadar air simplisia = x 100% berat sampel (g)

  No. Berat sampel (g) Volume awal (ml) Volume akhir (ml) 1. 5,043 1,90 2,10 2. 5,032 2,10 2,30 3. 5,021 2,30 2,50 volume akhir − volume awal

  % Kadar air = x 100% berat sampel

• 2,10 1,90

  1. Kadar air = = x 100% 4,00% 5,002

  30 2,10 - 2,

  2. Kadar air = x 100% = 3,98% 5,021

• 2,50 2,30 =

  3. Kadar air = x 100% 3,99% 5,015

• + + 4,00% 3,98% 3,99%

  3,99%

  % Rata-rata kadar air = =

  3

  Lampiran 14. Perhitungan kadar abu total ekstrak etanol daun Pugun Tanoh % Kadar abu total = berat abu (g) berat sampel (g)

x 100%

No. Berat sampel (g) Berat abu (g)

  1. 1,9780 0,0323 2. 1,9949 0,0370 3. 2,0059 0,0393

  1. Kadar abu total = 1,63% 100% x 1,9780

  0,0323 =

  2. Kadar abu total = 1,85% 100% x 1,9949 0,0370

  =

  3. Kadar abu total = 96% , 1 100% x

  2,0059 0,0393 =

  % Rata-rata kadar abu total = % 81 ,

  1

  3 1,96% 1,85% 1,63% =

  Lampiran 15. Perhitungan kadar abu tidak larut asam ekstrak etanol daun Pugun Tanoh berat abu (g)

  % Kadar abu total = x 100% berat sampel (g)

  No. Berat sampel (g) Berat abu (g) 1. 1,8992 0,0068 2. 1,9922 0,0071 3. 1,8255 0,0042

  0,0068

  1. Kadar abu total = = x 100% 0,36% 1,8992

  0,0071 =

  2. Kadar abu total = x 100% 0,36% 1,9922 0,0042

  3. Kadar abu total = = x 100% 0,23% 1,8255

  0,36% 0,36% 0,23%

  % Rata-rata kadar abu total = = , 32 %

  3

  Lampiran 16. Perhitungan kadar sari larut air ekstrak etanol daun Pugun Tanoh berat sari (g) 100

  % Kadar sari larut dalam air = x 100% x berat sampel (g)

  20 No. Berat sampel (g) Berat sari (g) 1. 5,015 0,590 2. 5,028 0,597 3. 5,019 0,592

  

0,590 100

  1. Kadar sari larut dalam air = = x x 100% 58,82% 5,015

  20

0,597 100

  2. Kadar sari larut dalam air = x x 100% = 59,37% 5,028

  20

0,592 100

  3. Kadar sari larut dalam air = = x x 100% 58,98% 5,019

  20 58,82% 59,37% 58,98%

  % Rata-rata kadar sari larut dalam air= = 59 , 06 %

  3

  Lampiran 17. Perhitungan kadar sari larut etanol ekstrak etanol daun Pugun Tanoh % Kadar sari larut dalam etanol =

berat sari (g)

berat sampel (g)

x

  100

  20 x

  100% No. Berat sampel (g) Berat sari (g) 1. 5,035 0,714 2. 5,019 0,703 3. 5,021 0,707

  a. Kadar sari larut dalam etanol = 70,90% 100% x

  20 100

x

5,035

  0,714 = b. Kadar sari larut dalam etanol = 70,03% 100% x

  20 100

x

5,019

  0,703 = c. Kadar sari larut dalam etanol =

  70,40% 100% x

  20 100

x

5,021

  0,707 = % Rata-rata kadar sari larut dalam etanol= %

  44 ,

  70

  3 70,40% 70,03% 70,90% =

  Konsentras i Asetilkolin (M)

  1

  2

  3 1 x 10-8 1,3514 2,4390 13,7255 5,8386 3,9559 3 x 10-8 5,4054 3,6538 19,6078 9,5572 5,0505 1 x 10-7 5,4054 4,8780 18,8235 9,7023 4,5631 3 x 10-7 6,0811 13,4146 19,6078 13,0345 3,9094 1 x 10-6 10,1351 40,2439 19,6078 23,3289 8,8886 3 x 10-6 25,0000 71,9512 31,3725 42,7746 14,7038 1 x 10-5 47,2973 91,4634 56,8627 65,2078 13,4151 3 x 10-5 79,7297 91,4634 80,3922 83,8618 3,8056 1 x 10-4 97,2973 91,4634 92,1569 93,6392 1,8400 3 x 10-4 97,9730 92,6829 88,2353 92,9637 2,8145 1 x 10-3 91,8919 95,1220 92,1569 93,0569 1,0354 3 x 10-3 100,0000 100,0000 100,0000 100,0000 0,0000

  % Kontraksi Ileum* Rerata SEM Lampiran 18. Data pengujian kontraksi seri konsentrasi asetilkolin terhadap otot polos ileum

  1. Tabel data pengujian kontraksi seri konsentrasi asetilkolin terhadap otot polos ileum Keterangan :

• = %kontraksi dihitung dari titik kontraksi maksimal yang dicapai oleh pemberian asetilkolin Media = larutan tirode

  Lampiran 18. (Lanjutan)

  80 ₁ − ) = × (

  80

  2

  1

  1 � − � + _{2 1}

  − Keterangan: X : Log. konsentrasi dengan respons tepat di bawah 80%

  1 X : Log. konsentrasi dengan respons tepat di atas 80%

  2 Y : %respons tepat di bawah 80%

  1 Y 2 : %respons tepat di atas 80%

  80 − 79,7297 = × . 80 � �−4 − (−4,5)�� + (−4,5) 97,2973

  − 79,7297 =

  80 −4,492

• 5

  80 EC = 3,22 x 10 M

  80 − 71,9512 = × . 80 � �−5 − (−5,5)�� + (−5,5) 91,4634

  − 71,9512 = 80 −5,294

• 6

  80 EC = 5,08 x 10 M

  80 − 56,8627 = × . 80 � �−4,5 − (−5)�� + (−5) 80,3922

  − 56,8627 =

  80 −4,508

• 5

  80 EC = 3,10 x 10 M

  −5 −6 −5

  

3,22 + 5,08 + 3,10

  10

  10

  10 Rerata EC 80 asetilkolin =

  3

• 5

  = 2,28 x 10 M

• 5

  10,7788 1,5215 1,0 26,3889 20,0000 19,2308 24,0964 25,6757 28,0000

  5,7461 Rerata SEM % Relaksasi Ileum* Keterangan :

  99,7713 4,5525 4,0 87,5000 110,0000 96,1538 126,5060 116,2162 112,0000 108,0627

  91,6422 3,8605 3,5 86,1111 102,5000 89,7436 116,8675 105,4054 98,0000

  80,8723 2,5126 3,0 81,9444 91,2500 83,3333 108,4337 91,8919 93,0000

  63,2099 3,3164 2,5 79,1667 88,7500 70,5128 80,7229 81,0811 85,0000

  42,7169 2,2022 2,0 75,0000 57,5000 56,4103 60,2410 58,1081 72,0000

  23,8986 1,4515 1,5 45,8333 40,0000 38,4615 42,1687 37,8378 52,0000

  6 0,5 13,8889 8,7500 8,9744 10,8434 16,2162 6,0000

  5

  4

  3

  2

  1

  M Dosis Ekstrak (mg/ml)

  Lampiran 19. Data pengujian efek relaksasi ekstrak etanol daun Pugun Tanoh pada kontraksi ileum melalui induksi asetilkolin 2,28 x 10

• 5
• = %relaksasi dihitung dari titik kontraksi maksimal yang dicapai oleh pemberian asetilkolin 2,28 x 10

  M Media = larutan tirode

• 5

  11,6324 4,0444 3 x 10-8 7,6923 26,6667 7,1429 44,0678 40,0000 57,5000

  4,9396 Rerata SEM % Relaksasi Ileum* Keterangan :

  4,9396 3 x 10-5 126,9231 120,0000 114,2857 130,5085 121,0000 148,7500 126,9112

  4,9396 1 x 10-5 126,9231 120,0000 114,2857 130,5085 121,0000 148,7500 126,9112

  4,9396 3 x 10-6 126,9231 120,0000 114,2857 130,5085 121,0000 148,7500 126,9112

  3,9734 1 x 10-6 126,9231 120,0000 114,2857 130,5085 121,0000 148,7500 126,9112

  10,3789 3 x 10-7 122,1154 114,2857 105,9524 123,7288 121,0000 135,0000 120,3471

  30,5116 8,3342 1 x 10-7 65,3846 71,4286 50,0000 93,2203 98,0000 120,0000 83,0056

  6 1 x 10-8 6,7308 2,8571 5,9524 15,2542 9,0000 30,0000

  5

  4

  3

  2

  1

  M Konsentras i Atropin Sulfat (M)

  Lampiran 20. Data Pengujian efek relaksasi atropin sulfat pada kontraksi ileum melalui induksi asetilkolin 2,28 x 10

• 5
• = %relaksasi dihitung dari titik kontraksi maksimal yang dicapai oleh pemberian asetilkolin 2,28 x 10

  M Media = larutan tirode

• 5
• 4

  25,9625 4,4606 1,0 27,8571 37,9747 41,1765 49,3151 56,8966 59,1837

  4,0433 Rerata SEM % Relaksasi Ileum* Keterangan :

  3,6477 4,0 124,2857 112,6582 126,4706 123,2877 100,0000 116,3265 117,1715

  5,5755 3,5 122,8571 106,3291 120,5882 117,8082 100,0000 110,2041 112,9645

  5,2893 3,0 120,0000 97,4684 113,2353 117,8082 84,4828 110,2041 107,1998

  84,4145 4,2297 2,5 109,2857 86,0759 100,0000 100,0000 77,5862 110,2041 97,1920

  69,2041 4,2919 2,0 93,5714 67,0886 85,2941 93,1507 77,5862 89,7959

  45,4006 4,8929 1,5 77,1429 51,8987 64,7059 72,6027 67,2414 81,6327

  6 0,5 18,5714 22,7848 16,1765 19,1781 36,2069 42,8571

  5

  4

  3

  2

  1

  M selama 20 menit Dosis Ekstrak (mg/ml)

  M dengan inkubasi awal menggunakan aspirin 10

  

Lampiran 21. Data efek relaksasi ekstrak etanol daun Pugun Tanoh terhadap kontraksi otot polos ileum oleh pemberian asetilkolin

2,28 x 10

• 5
• = %relaksasi dihitung dari titik kontraksi maksimal yang dicapai oleh pemberian asetilkolin 2,28 x 10

  M Media = larutan tirode

• 5

  Lampiran 22. Data AUC efek relaksasi ekstrak etanol daun Pugun Tanoh pada kontraksi ileum melalui induksi asetilkolin 2,28 x 10

  M

  1

  2

  3

  4

  5

  6

  7 1,0 10,0695 18,0556 30,2083 38,5417 40,2778 42,0139 43,4028 222,5694 2,0 7,1875 15,0000 24,3750 36,5625 45,0000 48,4375 53,1250 229,6875 3,0 7,0513 14,4231 23,7180 31,7308 38,4615 43,2692 46,4744 205,1282 4,0 8,7350 16,5663 25,6024 35,2410 47,2892 56,3253 60,8434 250,6025

  5,0 10,4730 15,8784 23,9865 34,7973 43,2433 49,3243 55,4054 233,1081 6,0 8,5000 20,0000 31,0000 39,2500 44,5000 47,7500 52,5000 243,5000 Rerata

  8,6694 16,6539 26,4817 36,0205 43,1286 47,8534 51,9585 230,7659 SEM

  0,5791 0,8462 1,3339 1,1193 1,3243 2,0769 2,5544 6,5492 Total AUC* Marmut

  Keterangan :

• 5
• = Area dibawah kurva dari %relaksasi dihitung dari titik kontraksi maksimal yang dicapai oleh pemberian asetilkolin 2,28 x 10

  M Media = larutan tirode

• 5
• 4

  5

  10,6913 Total AUC* Marmut Keterangan :

  SEM 2,2138 1,8322 2,0648 2,2567 2,6459 2,2737 1,9046

  Rerata 17,8408 28,6512 38,4046 45,4016 51,0979 55,0411 57,5340 293,9712

  3,0 14,3383 26,4706 37,5000 46,3235 53,3088 58,4559 61,7647 298,1618 4,0 17,1233 30,4795 41,4384 48,2877 54,4521 58,9041 60,2740 310,9589 5,0 23,2759 31,0345 36,2069 38,7931 40,5173 46,1207 50,0000 265,9483 6,0 25,5102 35,2041 42,8572 50,0000 55,1021 55,1021 56,6327 320,4082

  7 1,0 11,6071 26,2500 42,6786 50,7143 57,3214 60,7143 61,7857 311,0714 2,0 15,1899 22,4684 29,7468 38,2911 45,8861 50,9494 54,7468 257,2785

  6

  4

  3

  2

  1

  M selama 20 menit

  M dengan inkubasi awal menggunakan aspirin 10

  

Lampiran 23. Data AUC efek relaksasi ekstrak etanol daun Pugun Tanoh terhadap kontraksi otot polos ileum oleh pemberian asetilkolin

2,28 x 10

• 5
• = Area dibawah kurva dari %relaksasi dihitung dari titik kontraksi maksimal yang dicapai oleh pemberian asetilkolin 2,28 x 10

  M Media = larutan tirode

  Lampiran 24. Hasil uji korelasi efek relaksasi ekstrak etanol daun Pugun Tanoh

• 5

  pada kontraksi ileum melalui induksi asetilkolin 2,28 x 10 M Descriptive Statistics

  Mean Std. Deviation N Dosis 2.250 1.2247

  8 %relaksasi 65.119088 36.1624654

  8 Correlations _** Dosis %relaksasi

  Dosis Pearson Correlation 1 .988 Sig. (2-tailed) .000 N

  8 _**

  8 %relaksasi Pearson Correlation .988

  1 Sig. (2-tailed) .000 N

  8

  8 **. Correlation is significant at the 0.01 level (2-tailed).

  Model Summary

  Adjusted R Std. Error of the Model R R Square Square Estimate _a 1 .988 .975 .971 6.1216966 a. Predictors: (Constant), dosis

  Lampiran 25. Hasil uji-t independen nilai %relaksasi ekstrak etanol daun Pugun

• 7

  Tanoh 4 mg/ml dengan nilai %relaksasi atropin sulfat 3 x 10 M

• 5

  terhadap kontraksi otot polos ileum oleh asetilkolin 2,28 x 10 M Descriptives

  Perlakuan Statistic Std. Error

  % relaksasi ACh+Ekstrak Mean 108.062667 5.7461201 95% Confidence Lower Bound 93.291795 Interval for Mean

  Upper Bound 122.833539 5% Trimmed Mean 108.180407 Median 111.000000 Variance 198.107 Std. Deviation 14.0750621 Minimum 87.5000 Maximum 126.5060 Range 39.0060 Interquartile Range 24.7983 Skewness -.376 .845 Kurtosis -.555 1.741

  ACh+Atropin Mean 120.347050 3.9734305 sulfat 95% Confidence Lower Bound 110.133022 Interval for Mean

  Upper Bound 130.561078 5% Trimmed Mean 120.332700 Median 121.557700

  Lampiran 25. (Lanjutan) Tests of Normality

  Perlakuan Kolmogorov-Smirnov ^a Shapiro-Wilk Statistic df Sig. Statistic df Sig.

  % relaksasi ACh+Ekstrak .221 6 .200 ^* .963 6 .844 ACh+Atropin sulfat .197 6 .200 ^* .969 6 .887

  a. Lilliefors Significance Correction *. This is a lower bound of the true significance.

  Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean % relaksasi ACh+Ekstrak 6 108.062667 14.0750621 5.7461201

  ACh+Atropin sulfat 6 120.347050 9.7328772 3.9734305

  

Independent Samples Test

Levene's Test for

  Equality of

Variances t-test for Equality of Means

95% Confidence Interval of the Difference

  F Sig. t df Sig.

  (2- tailed) Mean Difference

  Std. Error Difference Lower Upper

  Lampiran 26. Hasil uji-t independen nilai %relaksasi ekstrak etanol daun Pugun Tanoh terhadap kontraksi otot polos ileum oleh asetilkolin 2,28 x

• 5 -4

  Descriptives

  Perlakuan Statistic Std. Error

  0.5 ACh+Ekstrak Mean 10.778817 1.5215237 mg/mL 95% Confidence Lower Bound 6.867615 Interval for Mean

  Upper Bound 14.690018 5% Trimmed Mean 10.742230 Median 9.908900 Variance 13.890 Std. Deviation 3.7269567 Minimum 6.0000 Maximum 16.2162 Range 10.2162 Interquartile Range 6.4082 Skewness .391 .845 Kurtosis -.747 1.741

  ACh+Aspirin10- Mean 25.962467 4.4605503

  4M+Ekstrak 95% Confidence Lower Bound 14.496257 Interval for Mean

  Upper Bound 37.428676 5% Trimmed Mean 25.567541 Median 20.981450

  Lampiran 26. (Lanjutan)

  1 mg/mL ACh+Ekstrak Mean 23.898633 1.4515248 95% Confidence Lower Bound 20.167370 Interval for Mean

  Upper Bound 27.629897 5% Trimmed Mean 23.930104 Median 24.886050 Variance 12.642 Std. Deviation 3.5554950 Minimum 19.2308 Maximum 28.0000 Range 8.7692 Interquartile Range 6.9840 Skewness -.473 .845 Kurtosis -1.707 1.741

  ACh+Aspirin10- Mean 45.400617 4.8929084

  4M+Ekstrak 95% Confidence Lower Bound 32.822995 Interval for Mean

  Upper Bound 57.978238 5% Trimmed Mean 45.609530 Median 45.245800 Variance 143.643 Std. Deviation 11.9851289 Minimum 27.8571 Maximum 59.1837

  Lampiran 26. (Lanjutan)

  1.5 ACh+Ekstrak Mean 42.716883 2.2021505 mg/mL 95% Confidence Lower Bound 37.056075 Interval for Mean

  Upper Bound 48.377691 5% Trimmed Mean 42.472215 Median 41.084350 Variance 29.097 Std. Deviation 5.3941451 Minimum 37.8378 Maximum 52.0000 Range 14.1622 Interquartile Range 9.0694 Skewness 1.179 .845 Kurtosis .753 1.741

  ACh+Aspirin10- Mean 69.204050 4.2918838

  4M+Ekstrak 95% Confidence Lower Bound 58.171412 Interval for Mean

  Upper Bound 80.236688 5% Trimmed Mean 69.474978 Median 69.922050 Variance 110.522 Std. Deviation 10.5129253 Minimum 51.8987 Maximum 81.6327

  Lampiran 26. (Lanjutan)

  2 mg/mL ACh+Ekstrak Mean 63.209900 3.3163864 95% Confidence Lower Bound 54.684857 Interval for Mean

  Upper Bound 71.734943 5% Trimmed Mean 62.932650 Median 59.174550 Variance 65.991 Std. Deviation 8.1234545 Minimum 56.4103 Maximum 75.0000 Range 18.5897 Interquartile Range 15.5224 Skewness .928 .845 Kurtosis -1.548 1.741

  ACh+Aspirin10- Mean 84.414483 4.2297189

  4M+Ekstrak 95% Confidence Lower Bound 73.541645 Interval for Mean

  Upper Bound 95.287322 5% Trimmed Mean 84.868315 Median 87.545000 Variance 107.343 Std. Deviation 10.3606531 Minimum 67.0886 Maximum 93.5714

  Lampiran 26. (Lanjutan)

  2.5 ACh+Ekstrak Mean 80.872250 2.5125704 mg/mL 95% Confidence Lower Bound 74.413482 Interval for Mean

  Upper Bound 87.331018 5% Trimmed Mean 81.010122 Median 80.902000 Variance 37.878 Std. Deviation 6.1545153 Minimum 70.5128 Maximum 88.7500 Range 18.2372 Interquartile Range 8.9343 Skewness -.717 .845 Kurtosis 1.394 1.741

  ACh+Aspirin10- Mean 97.191983 5.2892883

  4M+Ekstrak 95% Confidence Lower Bound 83.595435 Interval for Mean

  Upper Bound 110.788532 5% Trimmed Mean 97.558298 Median 100.000000 Variance 167.859 Std. Deviation 12.9560575 Minimum 77.5862 Maximum 110.2041

  Lampiran 26. (Lanjutan)

  3 mg/mL ACh+Ekstrak Mean 91.642217 3.8604810 95% Confidence Lower Bound 81.718534 Interval for Mean

  Upper Bound 101.565899 5% Trimmed Mean 91.248124 Median 91.570950 Variance 89.420 Std. Deviation 9.4562086 Minimum 81.9444 Maximum 108.4337 Range 26.4893 Interquartile Range 13.8723 Skewness 1.153 .845 Kurtosis 1.902 1.741

  ACh+Aspirin10- Mean 107.199800 5.5755376

  4M+Ekstrak 95% Confidence Lower Bound 92.867424 Interval for Mean

  Upper Bound 121.532176 5% Trimmed Mean 107.750733 Median 111.719700 Variance 186.520 Std. Deviation 13.6572222 Minimum 84.4828 Maximum 120.0000

  Lampiran 26. (Lanjutan)

  3.5 ACh+Ekstrak Mean 99.771267 4.5525168 mg/mL 95% Confidence Lower Bound 88.068650 Interval for Mean

  Upper Bound 111.473884 5% Trimmed Mean 99.580374 Median 100.250000 Variance 124.352 Std. Deviation 11.1513432 Minimum 86.1111 Maximum 116.8675 Range 30.7564 Interquartile Range 19.4355 Skewness .353 .845 Kurtosis -.300 1.741

  ACh+Aspirin10- Mean 112.964450 3.6477042

  4M+Ekstrak 95% Confidence Lower Bound 103.587728 Interval for Mean

  Upper Bound 122.341172 5% Trimmed Mean 113.135106 Median 114.006150 Variance 79.834 Std. Deviation 8.9350139 Minimum 100.0000 Maximum 122.8571

  Lampiran 26. (Lanjutan)

  4 mg/mL ACh+Ekstrak Mean 108.062667 5.7461201 95% Confidence Lower Bound 93.291795 Interval for Mean

  Upper Bound 122.833539 5% Trimmed Mean 108.180407 Median 111.000000 Variance 198.107 Std. Deviation 14.0750621 Minimum 87.5000 Maximum 126.5060 Range 39.0060 Interquartile Range 24.7983 Skewness -.376 .845 Kurtosis -.555 1.741

  ACh+Aspirin10- Mean 117.171450 4.0432550

  4M+Ekstrak 95% Confidence Lower Bound 106.777932 Interval for Mean

  Upper Bound 127.564968 5% Trimmed Mean 117.608800 Median 119.807100 Variance 98.087 Std. Deviation 9.9039115 Minimum 100.0000 Maximum 126.4706

  Lampiran 26. (Lanjutan) Tests of Normality _a

  Kolmogorov-Smirnov Shapiro-Wilk _* Perlakuan Statistic df Sig. Statistic df Sig. 0.5 mg/mL ACh+Ekstrak .186 6 .200 .963 6 .839

  ACh+Aspirin10- .281 6 .150 .842 6 .136 _*

  4M+Ekstrak 1 mg/mL ACh+Ekstrak .197 6 .200 .907 _* 6 .416

  ACh+Aspirin10- .165 6 .200 .954 6 .770 _*

  4M+Ekstrak _* 1.5 mg/mL ACh+Ekstrak .207 6 .200 .888 6 .308

  ACh+Aspirin10- .168 6 .200 .964 6 .851

  4M+Ekstrak 2 mg/mL ACh+Ekstrak .309 6 .075 .796 _* 6 .055

  ACh+Aspirin10- .200 6 .200 .885 6 .293 _*

  4M+Ekstrak _* 2.5 mg/mL ACh+Ekstrak .224 6 .200 .947 6 .719

  ACh+Aspirin10- .252 6 .200 .900 6 .375

  4M+Ekstrak 3 mg/mL ACh+Ekstrak .276

• _* 6 .170 .877

  6 .256 ACh+Aspirin10- .254 6 .200 .890 6 .319 _*

  4M+Ekstrak _* 3.5 mg/mL ACh+Ekstrak .149 6 .200 .971 6 .898

  ACh+Aspirin10- .206 6 .200 .940 6 .659

  Lampiran 26. (Lanjutan) Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean 0.5 mg/mL ACh+Ekstrak 6 10.778817 3.7269567 1.5215237

  ACh+Aspirin10-4M+Ekstrak 6 25.962467 10.9260721 4.4605503 1 mg/mL ACh+Ekstrak 6 23.898633 3.5554950 1.4515248

  ACh+Aspirin10-4M+Ekstrak 6 45.400617 11.9851289 4.8929084 1.5 mg/mL ACh+Ekstrak 6 42.716883 5.3941451 2.2021505

  ACh+Aspirin10-4M+Ekstrak 6 69.204050 10.5129253 4.2918838 2 mg/mL ACh+Ekstrak 6 63.209900 8.1234545 3.3163864

  ACh+Aspirin10-4M+Ekstrak 6 84.414483 10.3606531 4.2297189 2.5 mg/mL ACh+Ekstrak 6 80.872250 6.1545153 2.5125704

  ACh+Aspirin10-4M+Ekstrak 6 97.191983 12.9560575 5.2892883 3 mg/mL ACh+Ekstrak 6 91.642217 9.4562086 3.8604810

  ACh+Aspirin10-4M+Ekstrak 6 107.199800 13.6572222 5.5755376 3.5 mg/mL ACh+Ekstrak 6 99.771267 11.1513432 4.5525168

  ACh+Aspirin10-4M+Ekstrak 6 112.964450 8.9350139 3.6477042 4 mg/mL ACh+Ekstrak 6 108.062667 14.0750621 5.7461201

  ACh+Aspirin10-4M+Ekstrak 6 117.171450 9.9039115 4.0432550

  Independent Samples Test Levene's Test for

  Lampiran 26. (Lanjutan)

  1 Equal 8.631 .015 -4.213 10 .002 -21.5019833 5.1036729 -32.8736751 -10.1302915 mg/mL variances assumed Equal -4.213 5.873 .006 -21.5019833 5.1036729 -34.0558149 -8.9481517 variances not assumed

  1.5 Equal 1.966 .191 -5.491 10 .000 -26.4871667 4.8238712 -37.2354215 -15.7389118 mg/mL variances assumed Equal -5.491 7.462 .001 -26.4871667 4.8238712 -37.7521835 -15.2221498 variances not assumed

  2 Equal .218 .651 -3.945 10 .003 -21.2045833 5.3748433 -33.1804806 -9.2286861 mg/mL variances assumed Equal -3.945 9.461 .003 -21.2045833 5.3748433 -33.2735207 -9.1356460 variances not assumed

  2.5 Equal 3.806 .080 -2.787 10 .019 -16.3197333 5.8557306 -29.3671142 -3.2723525 mg/mL variances assumed Equal -2.787 7.147 .026 -16.3197333 5.8557306 -30.1087344 -2.5307322 variances not assumed

  3 Equal 1.455 .255 -2.294 10 .045 -15.5575833 6.7815878 -30.6679026 -.4472641 mg/mL variances assumed Equal -2.294 8.898 .048 -15.5575833 6.7815878 -30.9254013 -.1897654 variances not assumed

  3.5 Equal .125 .731 -2.262 10 .047 -13.1931833 5.8336228 -26.1913049 -.1950618 mg/mL variances assumed Equal -2.262 9.546 .048 -13.1931833 5.8336228 -26.2755448 -.1108219 variances not

  

Lampiran 27. Hasil uji-t independen nilai AUC dari %relaksasi ekstrak etanol

daun Pugun Tanoh terhadap kontraksi otot polos ileum oleh

• 5 -4

  asetilkolin 2,28 x 10 M dengan inkubasi aspirin 10 M dan tanpa inkubasi Descriptives

  Perlakuan Statistic Std. Error

  % relaksasi ACh+Ekstrak Mean 108.062667 5.7461201 95% Confidence Lower Bound 93.291795 Interval for Mean

  Upper Bound 122.833539 5% Trimmed Mean 108.180407 Median 111.000000 Variance 198.107 Std. Deviation 14.0750621 Minimum 87.5000 Maximum 126.5060 Range 39.0060 Interquartile Range 24.7983 Skewness -.376 .845 Kurtosis -.555 1.741

  ACh+Atropin Mean 120.347050 3.9734305 sulfat 95% Confidence Lower Bound 110.133022 Interval for Mean

  Upper Bound 130.561078 5% Trimmed Mean 120.332700

  Lampiran 27. (Lanjutan) Tests of Normality

  Perlakuan Kolmogorov-Smirnov ^a Shapiro-Wilk Statistic df Sig. Statistic df Sig.

  AUC ACh+Ekstrak .140 6 .200 ^* .975 6 .923 ACh+Aspirin10-

  4M+Ekstrak .242 6 .200 ^* .863 6 .198

  a. Lilliefors Significance Correction *. This is a lower bound of the true significance.

  Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean AUC ACh+Ekstrak 6 230.765950 16.0422637 6.5492267

  ACh+Aspirin10-4M+Ekstrak 6 293.971183 26.1882113 10.6912925

  Independent Samples Test Levene's Test for

  Equality of Variances t-test for Equality of Means 95% Confidence Interval of the Difference

  F Sig. T Df Sig.

  (2- tailed) Mean Difference

  Std. Error Difference Lower Upper

  Lampiran 28. Gambar preparasi organ ileum marmut ileum

  A B benang pengika A

  B

  Lampiran 29. Gambar alat organ bath A

  Lampiran 29. (Lanjutan)

C

  Lampiran 30. Gambar pola kontraksi dan relaksasi ileum marmut terisolasi

Gambar a. Pola kontraksi otot polos organ ileum terisolasi yang dikontraksi

dengan pemberian seri konsentrasi asetilkolin (10

• 8
• 3 M).
• – 3 x 10

  Lampiran 30. (Lanjutan)

Gambar c. Pola relaksasi setelah pemberian seri konsentrasi atropin sulfat pada

otot polos ileum terisolasi yang dikontraksi dengan asetilkolin 2,28 x

• 5 10 M.