Directory UMM :Data Elmu:jurnal:O:Organic Geochemistry:Vol32.Issue1.2001:
Organic Geochemistry 32 (2001) 11±20
www.elsevier.nl/locate/orggeochem
Molecular mass distribution and ¯uorescence characteristics of
dissolved organic ligands for copper(II) in Lake Biwa, Japan
F. Wu a,b,*, Eiichiro Tanoue a
a
Institute for Hydrospheric-Atmospheric Sciences, Nagoya University, Nagoya 464-8601, Japan
b
The State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry,
Chinese Academy of Sciences, Guiyang, People's Republic of China
Received 15 May 2000; accepted 24 October 2000
(returned to author for revision 16 October 2000)
Abstract
Molecular mass distribution and ¯uorescence characteristics of organic ligands for copper(II) in freshwater were, for
the ®rst time, investigated using ultra®ltration, immobilized metal ion anity chromatography (IMAC) and threedimensional excitation-emission matrix spectroscopy (3DEEM). Two types of humic-like ¯uorescence (Peaks A and B)
were found in all molecular ligand fractions, while an additional type of protein-like ¯uorescence (Peak C) was only
observed in the 0.1 mm-GF/F molecular ligand fractions. The Ex/Em maxima of Peak A in the
www.elsevier.nl/locate/orggeochem
Molecular mass distribution and ¯uorescence characteristics of
dissolved organic ligands for copper(II) in Lake Biwa, Japan
F. Wu a,b,*, Eiichiro Tanoue a
a
Institute for Hydrospheric-Atmospheric Sciences, Nagoya University, Nagoya 464-8601, Japan
b
The State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry,
Chinese Academy of Sciences, Guiyang, People's Republic of China
Received 15 May 2000; accepted 24 October 2000
(returned to author for revision 16 October 2000)
Abstract
Molecular mass distribution and ¯uorescence characteristics of organic ligands for copper(II) in freshwater were, for
the ®rst time, investigated using ultra®ltration, immobilized metal ion anity chromatography (IMAC) and threedimensional excitation-emission matrix spectroscopy (3DEEM). Two types of humic-like ¯uorescence (Peaks A and B)
were found in all molecular ligand fractions, while an additional type of protein-like ¯uorescence (Peak C) was only
observed in the 0.1 mm-GF/F molecular ligand fractions. The Ex/Em maxima of Peak A in the