Korelasi Kadar Homosistein Paternal dan Fragmentasi DNA Sperma pada Pasangan dengan Keguguran Berulang Idiopatik Dini

CORRELATION OF PATERNAL HOMOCYSTEINE LEVEL
AND SPERM DNA FRAGMENTATION IN COUPLE
WITH IDIOPATHIC RECURRENT EARLY PREGNANCY LOSS
ABSTRACT
Background: Recurrent pregnancy loss is the most traumatic event for
couple in the effort to obtain offspring. Various etiologies from women side
have been extensively studied, but more than half of them still remain
unknown. Embryo as the result of unification of sperm and oocyte, and any
defect in spermatogenesis will also influence the quality of embryo which will
determine the fate of the pregnancy. Routine semen analysis failed to support
the evidence of influence of defective sperm in recurrent pregnancy loss.
Currently, examination of sperm DNA fragmentation has been added to
evaluate the quality of sperm beside the routine semen analysis. We
hipothesized that high sperm DNA fragmentation play a role in incidence of
idiopathic recurrent early pregnancy loss. Beside that the cause of sperm
DNA fragmentation are numerous and remain controversial. We would like to
know whether paternal hyperhomocysteinemia has impact to high sperm DNA
fragmentation and incidence of idiophatic recurrent early pregnancy loss.
Objectives: To find the correlation of paternal homocysteine level with sperm
DNA fragmentation in couple with recurrent pregnancy loss.
Methods: An observational prospective analysis study has been conducted

by recruiting 40 cases of idiopathic recurrent early pregnancy loss and 40
cases of control from normal fertile population who attended to HFC, Fertility
Clinic, Division of Reproductive Endocrinology and Fertility, Department of
Obstetric & Gyneacology, Medical School, USU, H.Adam Malik Hospital
according to inclusion and exclusion criteria.
Blood and semen sample were collected for routine semen analysis, sperm
DNA fragmentation, serum and seminal homocysteine.
The results were analyzed to find the correlation between sperm DNA
fragmentation, serum homocysteine, seminal homocysteine and the
incidence of idiopathic recurrent early pregnancy loss.
Result: There was a significant correlation between incidence of idiopathic
recurrent early pregnancy loss with sperm DNA fragmentation (p