EFEK PEMBERIAN EKSTRAK ETANOL BUNGA BROKOLI (Brassica oleracea L. var. botrytis L) TERHADAP STRUKTUR HISTOLOGIS GINJAL MENCIT (Mus musculus) YANG DIINDUKSI PARASETAMOL.
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Efek Pemberian Ekstrak
Etanol Bunga Brokoli (
L. var
L) terhadap Struktur
Histologis Ginjal Mencit (
) yang Diinduksi Parasetamol. Skripsi.
Fakultas Kedokteran Universitas Sebelas Maret Surakarta.
: Ekstrak etanol bunga brokoli diketahui mengandung senyawa
saponin, flavonoid, alkaloid, terpenoid, steroid,
(CHB),
sulforafan dan iberin sebagai senyawa antioksidan. Penelitian ini bertujuan untuk
membuktikan efek pemberian ekstrak etanol bunga brokoli terhadap kerusakan
struktur histologis ginjal mencit yang diinduksi parasetamol dan untuk
mengetahui adakah perbedaan efek renoprotektor dari dosis ekstrak yang berbeda.
! "
#
: Penelitian ini bersifat eksperimental laboratorik dengan
rancangan
. Sampel 30 ekor mencit dibagi
dalam 5 kelompok, masing.masing kelompok terdiri dari 6 ekor. Teknik sampling
yang dipakai adalah
. Mencit Kelompok Kontrol Normal
(KKN) dan Kontrol parasetamol (KKparaset) diberi akuades selama 14 hari.
Mencit Kelompok Perlakuan 1 (KP1), Perlakuan 2 (KP2) dan Perlakuan 3 (KP3)
diberi ekstrak etanol bunga brokoli dengan dosis masing.masing 42 mg/20 gBB;
56 mg/20 gBB dan 70 mg/20 gBB. Parasetamol diberikan pada kelompok
KKparaset, KP1, KP2 dan KP3 pada hari ke 12, 13, dan 14. Pada hari ke.15,
mencit dikorbankan dan ginjal mencit dibuat preparat dengan metode Blok
Parafin dan pengecatan Hematoksilin Eosin (HE). Gambaran struktur histologis
ginjal mencit dinilai berdasarkan penjumlahan inti sel epitel tubulus proksimal
yang mengalami piknosis, karioreksis dan kariolisis. Data dianalisis menggunakan
uji
ANOVA dan dilanjutkan uji
LSD
(α = 0,05).
$ % #
: Rerata kerusakan sel epitel tubulus proksimal ginjal pada KKN,
KKparaset, KP1, KP2 dan KP3 berturut.turut adalah 6,08 ± 1,505 ; 36,00 ± 2,763.
30,42 ± 2,065; 23,67 ± 1,826; dan 14,92 ± 1,621. Hasil uji
ANOVA
dilanjutkan
LSD menunjukkan adanya perbedaan
yang bermakna antara KKN.KKparaset, KKparaset.KP1, KKparaset.KP2,
KKparaset.KP3, KP1.KP2, KP2.KP3, KP1. KP3, KKN.KP1, KKN.KP2 dan
KKN.KP3.
&
: Ekstrak etanol bunga brokoli memiliki efek proteksi terhadap struktur
histologis ginjal mencit yang diinduksi parasetamol. Peningkatan dosis ekstrak
etanol bunga brokoli dapat meningkatkan efek renoprotektor.
':
Ekstrak etanol bunga brokoli, parasetamol, kerusakan struktur
histologis ginjal
commit to user
iv
perpustakaan.uns.ac.id
digilib.uns.ac.id
(
Effect of Ethanolic Extract
of Broccoli Flowers (
L. var
L) Againts Paracetamol.
Induced Renal Histological Structure in Mice (
). Mini Thesis.
Faculty of Medicine, Sebelas Maret University, Surakarta.
' "
) The Ethanolic extract of broccoli flowers contains saponin,
flavonoid, alkaloid, terpenoid, steroid, cyano hydroxy butene, sulforafan and
iberin as an antioxidant compounds. The objectives of this research are to know
the effect of ethanolic extract of broccoli in preventing the renal histologist
structure damage in mice was induced by paracetamol. And also to know is there
any difference renoprotector effect in difference doses.
! " %) This was laboratory experimental with the post test only controlled
group design. Samples were 30 mices devided into 5 groups, each group has 6
mices. Sampling technique in this research was consecutive sampling. Mices of
normal control group KKN and parasetamol control group (KKparaset) will be
given aquadest for 14 days. The first (KP1), second (KP2) and third (KP3)
treatment group will be given with ethanolic extract of broccoli flowers dose 42
mg/20 gBB, 56 mg/20 gBB and 70 mg/20 gBB for 14 days. While the
paracetamol in toxic dose was given on to KKparaset, KP1, KP2 and KP3 on day
12, 13, and 14. On day 15, the mices was terminated and the kidneys was made
preparations by paraffin block methods and HE staining. Kidneys histological
structure features assessed by sum of proximal tubule cells of renal damage that
undergo pycnosis, karyorexys and karyolysis. Data were analized by One.Way
ANOVA test (α = 0,05) and continued by Post Hoc Multiple Comparisons LSD
test (α = 0,05).
% %: Mean of proximal tubulus epitel cells of renal damage on KKN,
KKparaset, KP1, KP2 and KP3 was 6.08 ± 1.505 ; 36.00 ± 2.763. 30.42 ± 2.065;
23.67 ± 1.826; dan 14.92 ± 1.621. The result of One.Way ANOVA and Post Hoc
Multiple Comparisons LSD showed there was significant difference between
group of KKN.KKparaset, KKparaset.KP1, KKparaset.KP2, KKparaset.KP3,
KP1.KP2, KP2.KP3, KP1. KP3, KKN.KP1, KKN.KP2 dan KKN.KP3.
(" ' % " : The ethanolic extract of broccoli flowers showed renoprotector effect
against paracetamol.induced renal histologic structure in mice. The increasing
dose of ethanolic extract of broccoli can increase renoprotector effect.
*+" %) Ethanolic extract of broccoli flower, paracetamol, renal histologic
structure damage
commit to user
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perpustakaan.uns.ac.id
digilib.uns.ac.id
Efek Pemberian Ekstrak
Etanol Bunga Brokoli (
L. var
L) terhadap Struktur
Histologis Ginjal Mencit (
) yang Diinduksi Parasetamol. Skripsi.
Fakultas Kedokteran Universitas Sebelas Maret Surakarta.
: Ekstrak etanol bunga brokoli diketahui mengandung senyawa
saponin, flavonoid, alkaloid, terpenoid, steroid,
(CHB),
sulforafan dan iberin sebagai senyawa antioksidan. Penelitian ini bertujuan untuk
membuktikan efek pemberian ekstrak etanol bunga brokoli terhadap kerusakan
struktur histologis ginjal mencit yang diinduksi parasetamol dan untuk
mengetahui adakah perbedaan efek renoprotektor dari dosis ekstrak yang berbeda.
! "
#
: Penelitian ini bersifat eksperimental laboratorik dengan
rancangan
. Sampel 30 ekor mencit dibagi
dalam 5 kelompok, masing.masing kelompok terdiri dari 6 ekor. Teknik sampling
yang dipakai adalah
. Mencit Kelompok Kontrol Normal
(KKN) dan Kontrol parasetamol (KKparaset) diberi akuades selama 14 hari.
Mencit Kelompok Perlakuan 1 (KP1), Perlakuan 2 (KP2) dan Perlakuan 3 (KP3)
diberi ekstrak etanol bunga brokoli dengan dosis masing.masing 42 mg/20 gBB;
56 mg/20 gBB dan 70 mg/20 gBB. Parasetamol diberikan pada kelompok
KKparaset, KP1, KP2 dan KP3 pada hari ke 12, 13, dan 14. Pada hari ke.15,
mencit dikorbankan dan ginjal mencit dibuat preparat dengan metode Blok
Parafin dan pengecatan Hematoksilin Eosin (HE). Gambaran struktur histologis
ginjal mencit dinilai berdasarkan penjumlahan inti sel epitel tubulus proksimal
yang mengalami piknosis, karioreksis dan kariolisis. Data dianalisis menggunakan
uji
ANOVA dan dilanjutkan uji
LSD
(α = 0,05).
$ % #
: Rerata kerusakan sel epitel tubulus proksimal ginjal pada KKN,
KKparaset, KP1, KP2 dan KP3 berturut.turut adalah 6,08 ± 1,505 ; 36,00 ± 2,763.
30,42 ± 2,065; 23,67 ± 1,826; dan 14,92 ± 1,621. Hasil uji
ANOVA
dilanjutkan
LSD menunjukkan adanya perbedaan
yang bermakna antara KKN.KKparaset, KKparaset.KP1, KKparaset.KP2,
KKparaset.KP3, KP1.KP2, KP2.KP3, KP1. KP3, KKN.KP1, KKN.KP2 dan
KKN.KP3.
&
: Ekstrak etanol bunga brokoli memiliki efek proteksi terhadap struktur
histologis ginjal mencit yang diinduksi parasetamol. Peningkatan dosis ekstrak
etanol bunga brokoli dapat meningkatkan efek renoprotektor.
':
Ekstrak etanol bunga brokoli, parasetamol, kerusakan struktur
histologis ginjal
commit to user
iv
perpustakaan.uns.ac.id
digilib.uns.ac.id
(
Effect of Ethanolic Extract
of Broccoli Flowers (
L. var
L) Againts Paracetamol.
Induced Renal Histological Structure in Mice (
). Mini Thesis.
Faculty of Medicine, Sebelas Maret University, Surakarta.
' "
) The Ethanolic extract of broccoli flowers contains saponin,
flavonoid, alkaloid, terpenoid, steroid, cyano hydroxy butene, sulforafan and
iberin as an antioxidant compounds. The objectives of this research are to know
the effect of ethanolic extract of broccoli in preventing the renal histologist
structure damage in mice was induced by paracetamol. And also to know is there
any difference renoprotector effect in difference doses.
! " %) This was laboratory experimental with the post test only controlled
group design. Samples were 30 mices devided into 5 groups, each group has 6
mices. Sampling technique in this research was consecutive sampling. Mices of
normal control group KKN and parasetamol control group (KKparaset) will be
given aquadest for 14 days. The first (KP1), second (KP2) and third (KP3)
treatment group will be given with ethanolic extract of broccoli flowers dose 42
mg/20 gBB, 56 mg/20 gBB and 70 mg/20 gBB for 14 days. While the
paracetamol in toxic dose was given on to KKparaset, KP1, KP2 and KP3 on day
12, 13, and 14. On day 15, the mices was terminated and the kidneys was made
preparations by paraffin block methods and HE staining. Kidneys histological
structure features assessed by sum of proximal tubule cells of renal damage that
undergo pycnosis, karyorexys and karyolysis. Data were analized by One.Way
ANOVA test (α = 0,05) and continued by Post Hoc Multiple Comparisons LSD
test (α = 0,05).
% %: Mean of proximal tubulus epitel cells of renal damage on KKN,
KKparaset, KP1, KP2 and KP3 was 6.08 ± 1.505 ; 36.00 ± 2.763. 30.42 ± 2.065;
23.67 ± 1.826; dan 14.92 ± 1.621. The result of One.Way ANOVA and Post Hoc
Multiple Comparisons LSD showed there was significant difference between
group of KKN.KKparaset, KKparaset.KP1, KKparaset.KP2, KKparaset.KP3,
KP1.KP2, KP2.KP3, KP1. KP3, KKN.KP1, KKN.KP2 dan KKN.KP3.
(" ' % " : The ethanolic extract of broccoli flowers showed renoprotector effect
against paracetamol.induced renal histologic structure in mice. The increasing
dose of ethanolic extract of broccoli can increase renoprotector effect.
*+" %) Ethanolic extract of broccoli flower, paracetamol, renal histologic
structure damage
commit to user
v
perpustakaan.uns.ac.id
digilib.uns.ac.id
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