Efek nefroprotektif pemberian jangka panjang infusa biji persea americana mill. terhadap kadar kreatinin dan gambaran histologi ginjal tikus terinduksi karbon tetraklorida.
PLAGIAT
PLAGIATMERUPAKAN
MERUPAKANTINDAKAN
TINDAKANTIDAK
TIDAKTERPUJI
TERPUJI
INTISARI
Penelitian ini bertujuan untuk mengetahui pengaruh nefroprotektif
pemberian jangka panjang infusa biji alpukat (Persea americana Mill.)
berdasarkan kadar kreatinin serum dan gambaran histologis ginjal tikus jantan
galur Wistar yang terinduksi karbon tetraklorida (CCl4), serta mendapatkan besar
dosis efektif pemberian infusa biji alpukat (Persea americana Mill.).
Jenis penelitian bersifat eksperimental murni dengan rancangan acak
lengkap pola searah. Penelitian ini menggunakan tikus jantan galur Wistar, umur
2-3 bulan, berat badan ± 150-250 gram. Tikus dibagi secara acak ke dalam enam
kelompok perlakuan, masing-masing kelompok terdiri dari 5 ekor tikus.
Kelompok I (kontrol nefrotoksin) diberi karbon tetraklorida 2 mL/kgBB secara
intraperitoneal. Kelompok II (kontrol negatif) diberi olive oil 2 mL/kgBB.
Kelompok III (kontrol infusa) diberikan infusa biji alpukat (Persea americana
Mill.) dosis 1142,86 mg/kgBB. Kelompok IV-VI (perlakuan) berturut-turut diberi
infusa biji alpukat (Persea americana Mill.) dengan dosis 360,71; 642,06; dan
1142,86 mg/kgBB secara peroral sekali sehari selama enam hari berturut-turut,
pada hari ke tujuh semua kelompok perlakuan diberi induksi karbon tetraklorida
dosis 2 mL/kgBB secara intraperitoneal. Empat puluh delapan jam pasca induksi
karbon tetraklorida, darah diambil melalui sinus orbitalis mata untuk diukur kadar
kreatinin serum dan pengambilan ginjal untuk dilakukan pencuplikan jaringan
ginjal. Kadar kreatinin serum dianalisis secara statistik dengan menggunakan One
Way ANOVA dan preparat histologis dianalisis serta dideskripsikan.
Berdasarkan hasil penelitan, infusa biji Persea americana Mill.
memberikan efek nefroprotektif dengan menurunkan kadar kreatinin serum pada
tikus yang terinduksi karbon tetraklorida. Gambaran histologis belum dapat
dibandingkan hasilnya karena gambaran hislotogis kelompok nefrotoksin tidak
mengalami perubahan yang berarti secara patologis. Jadi infusa biji Persea
americana Mill. dosis 360,71; 642,06; dan 1142,86 mg/kgBB memiliki efek
nefroprotektif berturut-turut 100%; 80,95%; dan 61,90% berdasarkan kadar
kreatinin serum. Dosis efektif infusa biji Persea americana Mill. yang
memberikan efek nefroprotektif paling efektif adalah 360,71 mg/kgBB.
Kata kunci
:
Persea americana Mill., infusa, nefroprotektif, karbon
tetraklorida, kreatinin serum
xix
PLAGIAT
PLAGIATMERUPAKAN
MERUPAKANTINDAKAN
TINDAKANTIDAK
TIDAKTERPUJI
TERPUJI
ABSTRACT
The purpose of this research is to determine the nephroprotective effect of
infusion of avocado’s seeds (Persea americana Mill.) based on creatinine serum
level and renal histology of male rats strained Wistar induced carbon tetrachloride
(CCl4), and also to determine the effective dose of infusa of avocado’s seeds
(Persea americana Mill.).
This study purely using randomized experimental design, complete with
its unidirectional pattern. The subject of these studies were male rats of Wistar
strain, aged 2-3 months, and ± 150-250 gram for its weight. Rats were divided
into six groups randomly, each group consist of five rats. Group I (nefrotoxin
control) was given carbon tetrachloride 2 mL/kgBW i.p. Group II (negative
control) was given olive oil 2 mL/kgBW. Group III (infusion control) was given
infusion of avocado’s seeds (Persea americana Mill.) with the highest dose
(1142.86 mg/kgBW). Group IV-VI were given infusion of avocado’s seeds
(Persea americana Mill.), the doses were 360,71; 642.06; and 1142.86 mg/kgBW
orally once a day during six days continuously and then in the seventh day all of
these groups were induced carbon tetrachloride 2 mL/kgBW i.p. Fourty eight
hours after induced carbon tetrachloride, the blood was collected from sinus
orbitalis to measure the creatinine serum level and also made the renal histology.
Creatinine serum level was analyzed statistically using ANOVA one way and the
renal histology was analyzed microscopically also described.
Based on these result of the research, seeds infusion of Persea americana
Mill. gave nephroprotective effect for reducing creatinine level serum of rats
induced carbon tetrachloride. Renal histology results cannot be compared because
renal histology of control nefrotoxin group do not change pathologically. Thus
dose infusion of avocado’s seeds (Persea americana Mill.) 360,71; 642.06; and
1142.86 mg/kgBW had nephroprotective 100%, 80.95%, and 61.90% based on
creatinine serum level. Effective dose of infusion of avocado’s seeds (Persea
americana Mill.) which gave the most effective nephroprotective effect was
360,71 mg/kgBW.
Keywords
:
Persea americana Mill., infusa, nephroprotective,
carbon tetrachloride, creatinine serum
xx
JURNAL FARMASI SAINS DAN KOMUNITAS, November 2013, hlm. 95-100
ISSN : 1693-5683
Vol. 10 No. 2
POTENSI TAPE SEBAGAI PANGANAN TRADISIONAL TERHADAP
EFEK HEPATOTOKSIK TIKUS TERINDUKSI KARBON-TETRAKLORIDA
GIDION KRISNADI YOSEPH, CHRISTIANA DESTIA ANGGRAENI, NI LUH PUTU
DIAN PRAWITA PUTRI, ABEDNEGO YOGA DWI PRASETYO, PHEBE HENDRA
Fakultas Farmasi Universitas Sanata Dharma Yogyakarta
Abstract: The purpose of this study was to determine the effect of “Tape” that has an alcoholic
compound, to the hepatotoxic effect in the rat induced Carbon Tetrachloride. And also determine
which fermentation time of the Tape can give effect to the hepatotoxic in the rat induced Carbon
tetrachloride. Healthy rats were randomly divided into 7 groups of 5 animals in each. Group 1
received carbon tetrachloride 2ml/kgBW treated intraperitoneal. Group 2-4 were given “Tape”
suspension 18g/kgBW once daily for 6 days with different fermentation time (3, 5, 7 days). Group
5-7 were given “Tape” suspension 18g/KgBW once daily for 6 days with different fermentation
time and carbon tetrachloride was given on the 7th day. Blood sample from all groups was
obtained by sinus orbitalis after 24 hours application for estimation the serum level of
transaminase. The result showed that “Tape” suspension had a potential effect to reduce the
hepatotoxicity induced by carbon tetrachloride. “Tape” suspension with 5 days fermentation
was significantly decreased serum level of transaminase (P
PLAGIATMERUPAKAN
MERUPAKANTINDAKAN
TINDAKANTIDAK
TIDAKTERPUJI
TERPUJI
INTISARI
Penelitian ini bertujuan untuk mengetahui pengaruh nefroprotektif
pemberian jangka panjang infusa biji alpukat (Persea americana Mill.)
berdasarkan kadar kreatinin serum dan gambaran histologis ginjal tikus jantan
galur Wistar yang terinduksi karbon tetraklorida (CCl4), serta mendapatkan besar
dosis efektif pemberian infusa biji alpukat (Persea americana Mill.).
Jenis penelitian bersifat eksperimental murni dengan rancangan acak
lengkap pola searah. Penelitian ini menggunakan tikus jantan galur Wistar, umur
2-3 bulan, berat badan ± 150-250 gram. Tikus dibagi secara acak ke dalam enam
kelompok perlakuan, masing-masing kelompok terdiri dari 5 ekor tikus.
Kelompok I (kontrol nefrotoksin) diberi karbon tetraklorida 2 mL/kgBB secara
intraperitoneal. Kelompok II (kontrol negatif) diberi olive oil 2 mL/kgBB.
Kelompok III (kontrol infusa) diberikan infusa biji alpukat (Persea americana
Mill.) dosis 1142,86 mg/kgBB. Kelompok IV-VI (perlakuan) berturut-turut diberi
infusa biji alpukat (Persea americana Mill.) dengan dosis 360,71; 642,06; dan
1142,86 mg/kgBB secara peroral sekali sehari selama enam hari berturut-turut,
pada hari ke tujuh semua kelompok perlakuan diberi induksi karbon tetraklorida
dosis 2 mL/kgBB secara intraperitoneal. Empat puluh delapan jam pasca induksi
karbon tetraklorida, darah diambil melalui sinus orbitalis mata untuk diukur kadar
kreatinin serum dan pengambilan ginjal untuk dilakukan pencuplikan jaringan
ginjal. Kadar kreatinin serum dianalisis secara statistik dengan menggunakan One
Way ANOVA dan preparat histologis dianalisis serta dideskripsikan.
Berdasarkan hasil penelitan, infusa biji Persea americana Mill.
memberikan efek nefroprotektif dengan menurunkan kadar kreatinin serum pada
tikus yang terinduksi karbon tetraklorida. Gambaran histologis belum dapat
dibandingkan hasilnya karena gambaran hislotogis kelompok nefrotoksin tidak
mengalami perubahan yang berarti secara patologis. Jadi infusa biji Persea
americana Mill. dosis 360,71; 642,06; dan 1142,86 mg/kgBB memiliki efek
nefroprotektif berturut-turut 100%; 80,95%; dan 61,90% berdasarkan kadar
kreatinin serum. Dosis efektif infusa biji Persea americana Mill. yang
memberikan efek nefroprotektif paling efektif adalah 360,71 mg/kgBB.
Kata kunci
:
Persea americana Mill., infusa, nefroprotektif, karbon
tetraklorida, kreatinin serum
xix
PLAGIAT
PLAGIATMERUPAKAN
MERUPAKANTINDAKAN
TINDAKANTIDAK
TIDAKTERPUJI
TERPUJI
ABSTRACT
The purpose of this research is to determine the nephroprotective effect of
infusion of avocado’s seeds (Persea americana Mill.) based on creatinine serum
level and renal histology of male rats strained Wistar induced carbon tetrachloride
(CCl4), and also to determine the effective dose of infusa of avocado’s seeds
(Persea americana Mill.).
This study purely using randomized experimental design, complete with
its unidirectional pattern. The subject of these studies were male rats of Wistar
strain, aged 2-3 months, and ± 150-250 gram for its weight. Rats were divided
into six groups randomly, each group consist of five rats. Group I (nefrotoxin
control) was given carbon tetrachloride 2 mL/kgBW i.p. Group II (negative
control) was given olive oil 2 mL/kgBW. Group III (infusion control) was given
infusion of avocado’s seeds (Persea americana Mill.) with the highest dose
(1142.86 mg/kgBW). Group IV-VI were given infusion of avocado’s seeds
(Persea americana Mill.), the doses were 360,71; 642.06; and 1142.86 mg/kgBW
orally once a day during six days continuously and then in the seventh day all of
these groups were induced carbon tetrachloride 2 mL/kgBW i.p. Fourty eight
hours after induced carbon tetrachloride, the blood was collected from sinus
orbitalis to measure the creatinine serum level and also made the renal histology.
Creatinine serum level was analyzed statistically using ANOVA one way and the
renal histology was analyzed microscopically also described.
Based on these result of the research, seeds infusion of Persea americana
Mill. gave nephroprotective effect for reducing creatinine level serum of rats
induced carbon tetrachloride. Renal histology results cannot be compared because
renal histology of control nefrotoxin group do not change pathologically. Thus
dose infusion of avocado’s seeds (Persea americana Mill.) 360,71; 642.06; and
1142.86 mg/kgBW had nephroprotective 100%, 80.95%, and 61.90% based on
creatinine serum level. Effective dose of infusion of avocado’s seeds (Persea
americana Mill.) which gave the most effective nephroprotective effect was
360,71 mg/kgBW.
Keywords
:
Persea americana Mill., infusa, nephroprotective,
carbon tetrachloride, creatinine serum
xx
JURNAL FARMASI SAINS DAN KOMUNITAS, November 2013, hlm. 95-100
ISSN : 1693-5683
Vol. 10 No. 2
POTENSI TAPE SEBAGAI PANGANAN TRADISIONAL TERHADAP
EFEK HEPATOTOKSIK TIKUS TERINDUKSI KARBON-TETRAKLORIDA
GIDION KRISNADI YOSEPH, CHRISTIANA DESTIA ANGGRAENI, NI LUH PUTU
DIAN PRAWITA PUTRI, ABEDNEGO YOGA DWI PRASETYO, PHEBE HENDRA
Fakultas Farmasi Universitas Sanata Dharma Yogyakarta
Abstract: The purpose of this study was to determine the effect of “Tape” that has an alcoholic
compound, to the hepatotoxic effect in the rat induced Carbon Tetrachloride. And also determine
which fermentation time of the Tape can give effect to the hepatotoxic in the rat induced Carbon
tetrachloride. Healthy rats were randomly divided into 7 groups of 5 animals in each. Group 1
received carbon tetrachloride 2ml/kgBW treated intraperitoneal. Group 2-4 were given “Tape”
suspension 18g/kgBW once daily for 6 days with different fermentation time (3, 5, 7 days). Group
5-7 were given “Tape” suspension 18g/KgBW once daily for 6 days with different fermentation
time and carbon tetrachloride was given on the 7th day. Blood sample from all groups was
obtained by sinus orbitalis after 24 hours application for estimation the serum level of
transaminase. The result showed that “Tape” suspension had a potential effect to reduce the
hepatotoxicity induced by carbon tetrachloride. “Tape” suspension with 5 days fermentation
was significantly decreased serum level of transaminase (P