Uji aktivitas antibakteri fraksi n-heksana, fraksi kloroform, dan fraksi etanol kulit buah manggis (garcinia mangostana l.) terhadap escherichia coli resisten amoksisilin.
PLAGIAT
PLAGIATMERUPAKAN
MERUPAKANTINDAKAN
TINDAKANTIDAK
TIDAKTERPUJI
TERPUJI
UJI AKTIVITAS ANTIBAKTERI FRAKSI N-HEKSANA, FRAKSI
KLOROFORM, DAN FRAKSI ETANOL KULIT BUAH MANGGIS
(Garcinia mangostana L.) TERHADAP Escherichia coli RESISTEN
AMOKSISILIN
Christiana Destia Anggraeni
108114074
INTISARI
Garcinia mangostana L. merupakan tanaman yang diketahui memiliki
aktivitas antibakteri terhadap Escherichia coli. Resistensi Escherichia coli terhadap
amoksisilin banyak ditemui di masyarakat sehingga dilakukan penelitian tentang
aktivitas antibakteri dari Garcinia mangostana L. terhadap Escherichia coli yang
resisten amoksisilin.
Penelitian ini merupakan eksperimental murni rancangan acak lengkap pola
searah. Kultur Escherichia coli resisten amoksisilin didapat dengan metode adaptif
gradual. Ekstraksi bertingkat dengan metode maserasi kulit buah manggis
menggunakan pelarut n-heksana, kloroform, dan etanol. Uji aktivitas antibakteri
masing-masing fraksi menggunakan metode difusi sumuran (variasi konsentrasi 25%;
12,5%, 6,25%, 3,125%; 1,5625%).
Hasil penelitian menunjukkan bahwa Escherichia coli dapat menjadi resisten
terhadap amoksisilin ditunjukkan dengan meningkatnya nilai KHM menjadi 10 kali
lipat, yaitu dari 4 µg/ml menjadi >42 µg/ml. Hasil dari uji aktivitas antibakteri
menunjukkan bahwa fraksi n-heksana dan kloroform tidak memiliki aktivitas
antibakteri terhadap Escherichia coli resisten amoksisilin, sedangkan fraksi etanol
mempunyai aktivitas antibakteri terhadap Escherichia coli resisten amoksisilin. Hasil
analisis statistik menggunakan uji ANOVA dilanjutkan dengan uji Post Hoc
menggunakan t-test menunjukkan bahwa konsentrasi 25%; 12,5%; 6,25% dan
3,125% fraksi etanol mempunyai aktivitas antibakteri yang berbeda bermakna
terhadap kontrol negatif.
Kata kunci: fraksi n-heksana, fraksi kloroform, fraksi etanol, Garcinia mangostana L,
antibakteri, Escherichia coli, resisten, amoksisilin.
PLAGIAT
PLAGIATMERUPAKAN
MERUPAKANTINDAKAN
TINDAKANTIDAK
TIDAKTERPUJI
TERPUJI
ABSTRACT
Garcinia mangostana L. is a plant that is known have antibacterial activity
against Escherichia coli. Escherichia coli resistant to Amoxicillin mostly found in
societies that antibacterial activity of Garcinia mangostana L against amoxicillin
resistant Escherichia coli is studied.
This study purely experimental, completely randomized design. amoxicillin
resistant Escherichia coli cultures obtained by gradual adaptive method. Terraced
extraction by maceration using n-hexane, chloroform, and ethanol as solvent.
Antibacterial activity test using diffusion method (variations concentration are 25%;
12,5%, 6,25%, 3,125%; 1,5625%).
The result showed that Escherichia coli can become resistant to amoxicillin
indicated by increasing MIC to 10 fold, from 4 µg/ml to >42 µg/ml. The result of
antibacterial activity test showed that n-hexane and chloroform fraction do not have
antibacterial activity againts amoxicillin resistant Escherichia coli, while the ethanol
fraction has antibacterial activity againts amoxicillin resistant Escherichia coli.
Statistical analysing using ANOVA followed by Post Hoc using t-test showed that
concentration 25%; 12,5%; 6,25%; and 3,125% of ethanol fraction have a significant
difference of antibacterial activity to the negative control.
Keyword: n-hexane fraction, chloroform fraction, ethanol fraction, Garcinia
mangostana L., antibacterial, Escherichia coli, resistance, amoxicillin
JURNAL FARMASI SAINS DAN KOMUNITAS, November 2013, hlm. 95-100
ISSN : 1693-5683
Vol. 10 No. 2
POTENSI TAPE SEBAGAI PANGANAN TRADISIONAL TERHADAP
EFEK HEPATOTOKSIK TIKUS TERINDUKSI KARBON-TETRAKLORIDA
GIDION KRISNADI YOSEPH, CHRISTIANA DESTIA ANGGRAENI, NI LUH PUTU
DIAN PRAWITA PUTRI, ABEDNEGO YOGA DWI PRASETYO, PHEBE HENDRA
Fakultas Farmasi Universitas Sanata Dharma Yogyakarta
Abstract: The purpose of this study was to determine the effect of “Tape” that has an alcoholic
compound, to the hepatotoxic effect in the rat induced Carbon Tetrachloride. And also determine
which fermentation time of the Tape can give effect to the hepatotoxic in the rat induced Carbon
tetrachloride. Healthy rats were randomly divided into 7 groups of 5 animals in each. Group 1
received carbon tetrachloride 2ml/kgBW treated intraperitoneal. Group 2-4 were given “Tape”
suspension 18g/kgBW once daily for 6 days with different fermentation time (3, 5, 7 days). Group
5-7 were given “Tape” suspension 18g/KgBW once daily for 6 days with different fermentation
time and carbon tetrachloride was given on the 7th day. Blood sample from all groups was
obtained by sinus orbitalis after 24 hours application for estimation the serum level of
transaminase. The result showed that “Tape” suspension had a potential effect to reduce the
hepatotoxicity induced by carbon tetrachloride. “Tape” suspension with 5 days fermentation
was significantly decreased serum level of transaminase (P
PLAGIATMERUPAKAN
MERUPAKANTINDAKAN
TINDAKANTIDAK
TIDAKTERPUJI
TERPUJI
UJI AKTIVITAS ANTIBAKTERI FRAKSI N-HEKSANA, FRAKSI
KLOROFORM, DAN FRAKSI ETANOL KULIT BUAH MANGGIS
(Garcinia mangostana L.) TERHADAP Escherichia coli RESISTEN
AMOKSISILIN
Christiana Destia Anggraeni
108114074
INTISARI
Garcinia mangostana L. merupakan tanaman yang diketahui memiliki
aktivitas antibakteri terhadap Escherichia coli. Resistensi Escherichia coli terhadap
amoksisilin banyak ditemui di masyarakat sehingga dilakukan penelitian tentang
aktivitas antibakteri dari Garcinia mangostana L. terhadap Escherichia coli yang
resisten amoksisilin.
Penelitian ini merupakan eksperimental murni rancangan acak lengkap pola
searah. Kultur Escherichia coli resisten amoksisilin didapat dengan metode adaptif
gradual. Ekstraksi bertingkat dengan metode maserasi kulit buah manggis
menggunakan pelarut n-heksana, kloroform, dan etanol. Uji aktivitas antibakteri
masing-masing fraksi menggunakan metode difusi sumuran (variasi konsentrasi 25%;
12,5%, 6,25%, 3,125%; 1,5625%).
Hasil penelitian menunjukkan bahwa Escherichia coli dapat menjadi resisten
terhadap amoksisilin ditunjukkan dengan meningkatnya nilai KHM menjadi 10 kali
lipat, yaitu dari 4 µg/ml menjadi >42 µg/ml. Hasil dari uji aktivitas antibakteri
menunjukkan bahwa fraksi n-heksana dan kloroform tidak memiliki aktivitas
antibakteri terhadap Escherichia coli resisten amoksisilin, sedangkan fraksi etanol
mempunyai aktivitas antibakteri terhadap Escherichia coli resisten amoksisilin. Hasil
analisis statistik menggunakan uji ANOVA dilanjutkan dengan uji Post Hoc
menggunakan t-test menunjukkan bahwa konsentrasi 25%; 12,5%; 6,25% dan
3,125% fraksi etanol mempunyai aktivitas antibakteri yang berbeda bermakna
terhadap kontrol negatif.
Kata kunci: fraksi n-heksana, fraksi kloroform, fraksi etanol, Garcinia mangostana L,
antibakteri, Escherichia coli, resisten, amoksisilin.
PLAGIAT
PLAGIATMERUPAKAN
MERUPAKANTINDAKAN
TINDAKANTIDAK
TIDAKTERPUJI
TERPUJI
ABSTRACT
Garcinia mangostana L. is a plant that is known have antibacterial activity
against Escherichia coli. Escherichia coli resistant to Amoxicillin mostly found in
societies that antibacterial activity of Garcinia mangostana L against amoxicillin
resistant Escherichia coli is studied.
This study purely experimental, completely randomized design. amoxicillin
resistant Escherichia coli cultures obtained by gradual adaptive method. Terraced
extraction by maceration using n-hexane, chloroform, and ethanol as solvent.
Antibacterial activity test using diffusion method (variations concentration are 25%;
12,5%, 6,25%, 3,125%; 1,5625%).
The result showed that Escherichia coli can become resistant to amoxicillin
indicated by increasing MIC to 10 fold, from 4 µg/ml to >42 µg/ml. The result of
antibacterial activity test showed that n-hexane and chloroform fraction do not have
antibacterial activity againts amoxicillin resistant Escherichia coli, while the ethanol
fraction has antibacterial activity againts amoxicillin resistant Escherichia coli.
Statistical analysing using ANOVA followed by Post Hoc using t-test showed that
concentration 25%; 12,5%; 6,25%; and 3,125% of ethanol fraction have a significant
difference of antibacterial activity to the negative control.
Keyword: n-hexane fraction, chloroform fraction, ethanol fraction, Garcinia
mangostana L., antibacterial, Escherichia coli, resistance, amoxicillin
JURNAL FARMASI SAINS DAN KOMUNITAS, November 2013, hlm. 95-100
ISSN : 1693-5683
Vol. 10 No. 2
POTENSI TAPE SEBAGAI PANGANAN TRADISIONAL TERHADAP
EFEK HEPATOTOKSIK TIKUS TERINDUKSI KARBON-TETRAKLORIDA
GIDION KRISNADI YOSEPH, CHRISTIANA DESTIA ANGGRAENI, NI LUH PUTU
DIAN PRAWITA PUTRI, ABEDNEGO YOGA DWI PRASETYO, PHEBE HENDRA
Fakultas Farmasi Universitas Sanata Dharma Yogyakarta
Abstract: The purpose of this study was to determine the effect of “Tape” that has an alcoholic
compound, to the hepatotoxic effect in the rat induced Carbon Tetrachloride. And also determine
which fermentation time of the Tape can give effect to the hepatotoxic in the rat induced Carbon
tetrachloride. Healthy rats were randomly divided into 7 groups of 5 animals in each. Group 1
received carbon tetrachloride 2ml/kgBW treated intraperitoneal. Group 2-4 were given “Tape”
suspension 18g/kgBW once daily for 6 days with different fermentation time (3, 5, 7 days). Group
5-7 were given “Tape” suspension 18g/KgBW once daily for 6 days with different fermentation
time and carbon tetrachloride was given on the 7th day. Blood sample from all groups was
obtained by sinus orbitalis after 24 hours application for estimation the serum level of
transaminase. The result showed that “Tape” suspension had a potential effect to reduce the
hepatotoxicity induced by carbon tetrachloride. “Tape” suspension with 5 days fermentation
was significantly decreased serum level of transaminase (P