Formulasi Sediaan Gel Ekstrak Buah Alpukat (Persea americana Mill.) dan Uji Aktivitasnya terhadap Pertumbuhan Rambut Tikus Putih
LAMPIRAN
(2)
(3)
Lampiran 3. Gambar Bahan dan Alat
Buah alpukat Irisan buah alpukat yang telah kering
Serbuk buah alpukat Ekstrak buah alpukat
(4)
Lampiran 3. (Lanjutan)
Viskometer Brookfield Hair analyzer
(5)
Lampiran 4. Gambar Sediaan Gel Ekstrak Buah Alpukat
Setelah selesai dibuat
Setelah 12 minggu penyimpanan
Formula 3% Formula 5% Formula 7% Minoksidil Basis
Basis Minoksidil
Formula 7% Formula 5%
(6)
Lampiran 5. Gambar Hasil Uji Homogenitas
Setelah selesai dibuat
(7)
Lampiran 6. Gambar salah satu contoh hasil pengukuran ketebalan rambut tikus putih
- Minggu I
- Minggu II
(8)
(9)
Lampiran 7. Gambar hasil pertumbuhan rambut tikus kontrol normal
Hari ke-
Tikus 1 Tikus 2 Tikus 3 Tikus 4
7
14
(10)
Lampiran 8. Gambar hasil pertumbuhan rambut tikus kontrol negatif
Hari ke-
Tikus 1 Tikus 2 Tikus 3 Tikus 4
7
14
(11)
Lampiran 9. Gambar hasil pertumbuhan rambut tikus formula 3%
Hari ke-
Tikus 1 Tikus 2 Tikus 3 Tikus 4
7
14
(12)
Lampiran 10. Gambar hasil pertumbuhan rambut tikus formula 5%
Hari ke-
Tikus 1 Tikus 2 Tikus 3 Tikus 4
7
14
(13)
Lampiran 11. Gambar hasil pertumbuhan rambut tikus formula 7%
Hari ke-
Tikus 1 Tikus 2 Tikus 3 Tikus 4
7
14
(14)
Lampiran 12. Gambar hasil pertumbuhan rambut tikus kontrol positif
Hari ke-
Tikus 1 Tikus 2 Tikus 3 Tikus 4
7
(15)
21
Lampiran 13. Data hasil perhitungan secara statistik
A. Ketebalan Rambut
- Minggu I
Tests of Normality
kelompok Kolmogorov-Smirnov
a
Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
Minggu I
kontrol normal .269 4 . .844 4 .207
kontrol negatif .192 4 . .971 4 .850
formula 3% .303 4 . .791 4 .086
formula 5% .283 4 . .863 4 .272
formula 7% .307 4 . .729 4 .024
kontrol positif .441 4 . .630 4 .001
(16)
Test of Homogeneity of Variances Minggu I
Levene Statistic df1 df2 Sig.
3.199 5 18 .031
Test Statisticsa,b
Minggu I
Chi-Square 17.580
df 5
Asymp. Sig. .004
a. Kruskal Wallis Test
b. Grouping Variable: kelompok Lampiran 13. (Lanjutan)
Mann-Whitney Test
kelompok Sig.
kontrol normal kontrol negatif .663
formula 3% .297
formula 5% .038
formula 7% .019
kontrol positif .018
kontrol negatif kontrol normal .663
formula 3% .384
formula 5% .108
formula 7% .037
kontrol positif .018
formula 3% kontrol normal .297
kontrol negatif .384
formula 5% .065
formula 7% .019
kontrol positif .017
formula 5% kontrol normal .038
kontrol negatif .108
(17)
formula 7% .134
kontrol positif .017
formula 7% kontrol normal .019
kontrol negatif .018
formula 3% .019
formula 5% .134
kontrol positif .096
kontrol positif kontrol normal .018
kontrol negatif .018
formula 3% .017
formula 5% .017
formula 7% .096
Lampiran 13. (Lanjutan)
- Minggu II
Test of Homogeneity of Variances Minggu II
Levene Statistic df1 df2 Sig.
11.916 5 18 .000
Tests of Normality
kelompok
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
Minggu II kontrol normal .283 4 . .863 4 .024
kontrol negatif .307 4 . .729 4 .272
formula 3% .307 4 . .729 4 .024
formula 5% .250 4 . .945 4 .683
formula 7% .252 4 . .916 4 .513
kontrol positif .300 4 . .842 4 .200
(18)
Test Statisticsa,b
Minggu II
Chi-Square 21.693
Df 5
Asymp. Sig. .001
a. Kruskal Wallis Test
b. Grouping Variable: kelompok
Lampiran 13. (Lanjutan)
Mann-Whitney Test
kelompok Sig.
kontrol normal kontrol negatif 1.000
formula 3% .018
formula 5% .019
formula 7% .019
kontrol positif .019
kontrol negatif kontrol normal 1.000
formula 3% .019
formula 5% .019
formula 7% .020
kontrol positif .019
formula 3% kontrol normal .018
kontrol negatif .019
formula 5% .019
formula 7% .019
kontrol positif .019
formula 5% kontrol normal .019
(19)
formula 3% .018
formula 7% .020
kontrol positif .019
formula 7% kontrol normal .019
kontrol negatif .020
formula 3% .019
formula 5% .020
kontrol positif .058
kontrol positif kontrol normal .019
kontrol negatif .019
formula 3% .019
formula 5% .019
formula 7% .058
Lampiran 13. (Lanjutan)
- Minggu III
Tests of Normality
Kelompok
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
Minggu III kontrol normal .252 4 . .916 4 .513
kontrol negatif .251 4 . .927 4 .574
formula 3% .304 4 . .811 4 .123
formula 5% .298 4 . .849 4 .224
formula 7% .237 4 . .939 4 .650
kontrol positif .198 4 . .958 4 .764
a. Lilliefors Significance Correction
Test of Homogeneity of Variances Minggu III
(20)
Test of Homogeneity of Variances Minggu III
Levene Statistic df1 df2 Sig.
1.906 5 18 .143
Lampiran 13. (Lanjutan)
Tukey HSD (I) kelompok (J) kelompok
Mean Difference
(I-J) Sig.
kontrol normal kontrol negatif .001250 .987
formula 3% -.007000* .024
formula 5% -.022500* .000
formula 7% -.026000* .000
kontrol positif -.032500* .000
kontrol negatif kontrol normal -.001250 .987
formula 3% -.008250* .006
formula 5% -.023750* .000
formula 7% -.027250* .000
kontrol positif -.033750* .000
formula 3% kontrol normal .007000* .024
kontrol negatif .008250* .006
formula 5% -.015500* .000
ANOVA Minggu III
Sum of Squares df Mean Square F Sig.
Between Groups .004 5 .001 105.870 .000
Within Groups .000 18 .000
(21)
formula 7% -.019000* .000
kontrol positif -.025500* .000
formula 5% kontrol normal .022500* .000
kontrol negatif .023750* .000
formula 3% .015500* .000
formula 7% -.003500 .507
kontrol positif -.010000* .001
formula 7% kontrol normal .026000* .000
kontrol negatif .027250* .000
formula 3% .019000* .000
formula 5% .003500 .507
kontrol positif -.006500* .040
kontrol positif kontrol normal .032500* .000
kontrol negatif .033750* .000
formula 3% .025500* .000
formula 5% .010000* .001
formula 7% .006500* .040
*. The mean difference is significant at the 0.05 level.
Lampiran 13. (Lanjutan)
B. Panjang Rambut
Tests of Normality
Kelompok
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
Minggu I kontrol normal .250 4 . .945 4 .683
kontrol negatif .275 4 . .887 4 .371
formula 3% .333 4 . .804 4 .111
formula 5% .226 4 . .976 4 .880
formula 7% .250 4 . .953 4 .734
kontrol positif .215 4 . .946 4 .689
Minggu II kontrol normal .192 4 . .971 4 .850
(22)
formula 3% .238 4 . .895 4 .407
formula 5% .232 4 . .912 4 .492
formula 7% .155 4 . .998 4 .995
kontrol positif .221 4 . .912 4 .494
Minggu II kontrol normal .214 4 . .950 4 .717
kontrol negatif .256 4 . .954 4 .740
formula 3% .234 4 . .910 4 .483
formula 5% .188 4 . .977 4 .885
formula 7% .320 4 . .888 4 .374
kontrol positif .252 4 . .858 4 .255
a. Lilliefors Significance Correction
Test of Homogeneity of Variances
Levene Statistic df1 df2 Sig.
Minggu I 2.385 5 18 .079
Minggu II 2.068 5 18 .117
Minggu III 1.892 5 18 .146
Lampiran 13. (Lanjutan)
ANOVA
Sum of Squares df Mean Square F Sig.
Minggu I Between Groups 36.693 5 7.339 159.513 .000
Within Groups .828 18 .046
Total 37.522 23
Minggu II Between Groups 71.080 5 14.216 92.482 .000
Within Groups 2.767 18 .154
Total 73.847 23
Minggu III Between Groups 123.902 5 24.780 48.639 .000
Within Groups 9.171 18 .509
(23)
Lampiran 13. (Lanjutan)
Minggu I Tukey HSD (I) kelompok (J) kelompok
Mean Difference
(I-J) Sig.
kontrol normal kontrol negatif -.28750 .436
formula 3% -1.21250* .000
formula 5% -1.48750* .000
formula 7% -2.30000* .000
kontrol positif -3.67500* .000
kontrol negatif kontrol normal .28750 .436
formula 3% -.92500* .000
formula 5% -1.20000* .000
formula 7% -2.01250* .000
kontrol positif -3.38750* .000
formula 3% kontrol normal 1.21250* .000
(24)
formula 5% -.27500 .482
formula 7% -1.08750* .000
kontrol positif -2.46250* .000
formula 5% kontrol normal 1.48750* .000
kontrol negatif 1.20000* .000
formula 3% .27500 .482
formula 7% -.81250* .001
kontrol positif -2.18750* .000
formula 7% kontrol normal 2.30000* .000
kontrol negatif 2.01250* .000
formula 3% 1.08750* .000
formula 5% .81250* .001
kontrol positif -1.37500* .000 kontrol positif kontrol normal 3.67500* .000
kontrol negatif 3.38750* .000
formula 3% 2.46250* .000
formula 5% 2.18750* .000
formula 7% 1.37500* .000
*. The mean difference is significant at the 0.05 level.
Lampiran 13. (Lanjutan)
Minggu II Tukey HSD (I) kelompok (J) kelompok
Mean Difference
(I-J) Sig.
kontrol normal kontrol negatif .02500 1.000
formula 3% -1.43750* .001
formula 5% -2.52500* .000
formula 7% -3.05000* .000
kontrol positif -4.82500* .000
kontrol negatif kontrol normal -.02500 1.000
formula 3% -1.46250* .001
formula 5% -2.55000* .000
formula 7% -3.07500* .000
kontrol positif -4.85000* .000
(25)
kontrol negatif 1.46250* .001
formula 5% -1.08750* .011
formula 7% -1.61250* .000
kontrol positif -3.38750* .000
formula 5% kontrol normal 2.52500* .000
kontrol negatif 2.55000* .000
formula 3% 1.08750* .011
formula 7% -.52500 .437
kontrol positif -2.30000* .000
formula 7% kontrol normal 3.05000* .000
kontrol negatif 3.07500* .000
formula 3% 1.61250* .000
formula 5% .52500 .437
kontrol positif -1.77500* .000
kontrol positif kontrol normal 4.82500* .000
kontrol negatif 4.85000* .000
formula 3% 3.38750* .000
formula 5% 2.30000* .000
formula 7% 1.77500* .000
*. The mean difference is significant at the 0.05 level.
Lampiran 13. (Lanjutan)
Minggu III Tukey HSD (I) kelompok (J) kelompok
Mean Difference
(I-J) Sig.
kontrol normal kontrol negatif -.50250 .913
formula 3% -2.85000* .000
formula 5% -3.93500* .000
formula 7% -4.67250* .000
kontrol positif -6.47500* .000
kontrol negatif kontrol normal .50250 .913
formula 3% -2.34750* .002
formula 5% -3.43250* .000
formula 7% -4.17000* .000
kontrol positif -5.97250* .000
(26)
kontrol negatif 2.34750* .002
formula 5% -108.500 .307
formula 7% -1.82250* .021
kontrol positif -3.62500* .000
formula 5% kontrol normal 3.93500* .000
kontrol negatif 3.43250* .000
formula 3% 108.500 .307
formula 7% -.73750 .691
kontrol positif -2.54000* .001
formula 7% kontrol normal 4.67250* .000
kontrol negatif 4.17000* .000
formula 3% 1.82250* .021
formula 5% .73750 .691
kontrol positif -1.80250* .023
kontrol positif kontrol normal 6.47500* .000
kontrol negatif 5.97250* .000
formula 3% 3.62500* .000
formula 5% 2.54000* .001
formula 7% 1.80250* .023
(1)
formula 7% -.019000* .000
kontrol positif -.025500* .000
formula 5% kontrol normal .022500* .000
kontrol negatif .023750* .000
formula 3% .015500* .000
formula 7% -.003500 .507
kontrol positif -.010000* .001
formula 7% kontrol normal .026000* .000
kontrol negatif .027250* .000
formula 3% .019000* .000
formula 5% .003500 .507
kontrol positif -.006500* .040
kontrol positif kontrol normal .032500* .000
kontrol negatif .033750* .000
formula 3% .025500* .000
formula 5% .010000* .001
formula 7% .006500* .040
*. The mean difference is significant at the 0.05 level.
Lampiran 13. (Lanjutan)
B. Panjang Rambut
Tests of Normality
Kelompok
Kolmogorov-Smirnova Shapiro-Wilk Statistic df Sig. Statistic df Sig. Minggu I kontrol normal .250 4 . .945 4 .683
kontrol negatif .275 4 . .887 4 .371
formula 3% .333 4 . .804 4 .111
formula 5% .226 4 . .976 4 .880
formula 7% .250 4 . .953 4 .734
kontrol positif .215 4 . .946 4 .689 Minggu II kontrol normal .192 4 . .971 4 .850 kontrol negatif .200 4 . .973 4 .861
(2)
formula 3% .238 4 . .895 4 .407
formula 5% .232 4 . .912 4 .492
formula 7% .155 4 . .998 4 .995
kontrol positif .221 4 . .912 4 .494 Minggu II kontrol normal .214 4 . .950 4 .717 kontrol negatif .256 4 . .954 4 .740
formula 3% .234 4 . .910 4 .483
formula 5% .188 4 . .977 4 .885
formula 7% .320 4 . .888 4 .374
kontrol positif .252 4 . .858 4 .255 a. Lilliefors Significance Correction
Test of Homogeneity of Variances
Levene Statistic df1 df2 Sig.
Minggu I 2.385 5 18 .079
Minggu II 2.068 5 18 .117
Minggu III 1.892 5 18 .146
Lampiran 13. (Lanjutan)
ANOVA
Sum of Squares df Mean Square F Sig.
Minggu I Between Groups 36.693 5 7.339 159.513 .000
Within Groups .828 18 .046
Total 37.522 23
Minggu II Between Groups 71.080 5 14.216 92.482 .000
Within Groups 2.767 18 .154
Total 73.847 23
Minggu III Between Groups 123.902 5 24.780 48.639 .000
Within Groups 9.171 18 .509
(3)
Lampiran 13. (Lanjutan)
Minggu I
Tukey HSD
(I) kelompok (J) kelompok
Mean Difference
(I-J) Sig.
kontrol normal kontrol negatif -.28750 .436
formula 3% -1.21250* .000
formula 5% -1.48750* .000
formula 7% -2.30000* .000
kontrol positif -3.67500* .000 kontrol negatif kontrol normal .28750 .436
formula 3% -.92500* .000
formula 5% -1.20000* .000
formula 7% -2.01250* .000
kontrol positif -3.38750* .000
formula 3% kontrol normal 1.21250* .000
(4)
formula 5% -.27500 .482
formula 7% -1.08750* .000
kontrol positif -2.46250* .000
formula 5% kontrol normal 1.48750* .000
kontrol negatif 1.20000* .000
formula 3% .27500 .482
formula 7% -.81250* .001
kontrol positif -2.18750* .000
formula 7% kontrol normal 2.30000* .000
kontrol negatif 2.01250* .000
formula 3% 1.08750* .000
formula 5% .81250* .001
kontrol positif -1.37500* .000 kontrol positif kontrol normal 3.67500* .000 kontrol negatif 3.38750* .000
formula 3% 2.46250* .000
formula 5% 2.18750* .000
formula 7% 1.37500* .000
*. The mean difference is significant at the 0.05 level.
Lampiran 13. (Lanjutan)
Minggu II
Tukey HSD
(I) kelompok (J) kelompok
Mean Difference
(I-J) Sig.
kontrol normal kontrol negatif .02500 1.000
formula 3% -1.43750* .001
formula 5% -2.52500* .000
formula 7% -3.05000* .000
kontrol positif -4.82500* .000
kontrol negatif kontrol normal -.02500 1.000
formula 3% -1.46250* .001
formula 5% -2.55000* .000
formula 7% -3.07500* .000
kontrol positif -4.85000* .000
(5)
kontrol negatif 1.46250* .001
formula 5% -1.08750* .011
formula 7% -1.61250* .000
kontrol positif -3.38750* .000
formula 5% kontrol normal 2.52500* .000
kontrol negatif 2.55000* .000
formula 3% 1.08750* .011
formula 7% -.52500 .437
kontrol positif -2.30000* .000
formula 7% kontrol normal 3.05000* .000
kontrol negatif 3.07500* .000
formula 3% 1.61250* .000
formula 5% .52500 .437
kontrol positif -1.77500* .000
kontrol positif kontrol normal 4.82500* .000
kontrol negatif 4.85000* .000
formula 3% 3.38750* .000
formula 5% 2.30000* .000
formula 7% 1.77500* .000
*. The mean difference is significant at the 0.05 level.
Lampiran 13. (Lanjutan)
Minggu III
Tukey HSD
(I) kelompok (J) kelompok
Mean Difference
(I-J) Sig.
kontrol normal kontrol negatif -.50250 .913
formula 3% -2.85000* .000
formula 5% -3.93500* .000
formula 7% -4.67250* .000
kontrol positif -6.47500* .000 kontrol negatif kontrol normal .50250 .913
formula 3% -2.34750* .002
formula 5% -3.43250* .000
formula 7% -4.17000* .000
kontrol positif -5.97250* .000
(6)
kontrol negatif 2.34750* .002
formula 5% -108.500 .307
formula 7% -1.82250* .021
kontrol positif -3.62500* .000
formula 5% kontrol normal 3.93500* .000
kontrol negatif 3.43250* .000
formula 3% 108.500 .307
formula 7% -.73750 .691
kontrol positif -2.54000* .001
formula 7% kontrol normal 4.67250* .000
kontrol negatif 4.17000* .000
formula 3% 1.82250* .021
formula 5% .73750 .691
kontrol positif -1.80250* .023 kontrol positif kontrol normal 6.47500* .000
kontrol negatif 5.97250* .000
formula 3% 3.62500* .000
formula 5% 2.54000* .001
formula 7% 1.80250* .023