Larutan Vitamin C Vitamin E Larutan Vitamin E

  

Lampiran A. Dokumentasi Gambar Pengukuran Diameter Tubulus Seminiferus

Testis Mencit

Penampang melintang tubulus seminiferus testis setelah pemberian vitamin C

dan E pada mencit yang dipajankan monosodium glutamat (MSG), pewarnaan

HE, Perbesaran 400x, (Konversi 20:1).

Lampiran B. Pembuatan Larutan MSG, Vitamin C dan E

  Serbuk MSG Ditimbang sebanyak 100 mg Dimasukkan ke dalam beaker glass

Ditambahkan aquadest sebanyak 0,2 ml

Diaduk dengan menggunakan spatula Larutan MSG

  Serbuk Vitamin C Ditimbang sebanyak 6,5 mg Dimasukkan ke dalam beaker glass

Ditambahkan aquadest sebanyak 0,2 ml

Diaduk dengan menggunakan spatula Larutan Vitamin C

  Vitamin E Diambil sebanyak0,65 mg Dilarutkan dengan minyak jarak “Oleum Riccini” 0,3 ml Larutan Vitamin E

Lampiran C. Pembuatan Preparat Histologis Testis

  Dibilas dengan NaCl 0,9% Difiksasi dalam BOUIN selama 1 malam Washing dalam alkohol 70% Dehidrasi dengan alkohol konsentrasi bertingkat mulai 70%, 80%, 96%, dan 100% Clearing dalam xylol Infiltrasi Embedding (penanaman) organ dalam cetakan kemudian dituangkan parafin murni, dibiarkan hingga didapatkan blok parafin

  Cutting (pemotongan) menggunakan mikrotom sehingga didapatkan pita-pita parafin Attaching (penempelan) pita parafin pada objek glass Deparafinasi dengan mencelupkan objek dalam xylol Dealkoholisasi dalam alkohol menurun dari 100%, 96%, 80%, hingga 70%

  Pewarnaan dengan mencelupkan dalam Hematoxilin selama 3-7 menit kemudian dibilas dengan air mengalir. Selanjutnya dicelupkan dalam alkohol 70%, lalu dicelupkan dalam Eosin selama 1-3 menit.

  Mounting yaitu menutup preparat dengan gelas penutup yang sebelumnya diberi Canada balsam Diberi label dan siap diamati

  Lampiran D. Dokumentasi Penelitian Vitamin E Castrol Oil Kandang Mencit Jarum Gavage Pemberian Perlakuan Pengambilan Organ

LAMPIRAN E. Data dan Analisis Statistik Berat Testis Mencit Rataan Berat Testis setelah Pemberian Vitamin C dan E pada mencit (Mus

P4 0,15 0,12 0,13 0,14 0,15 0,14 ± 0,01 Hasil Uji Statistik Berat Testis Mencit

  a

  Shapiro-Wilk Statistic df Sig. Statistic df Sig. berat_testis k- .367 5 .026 .684 5 .006 k+ .231

  musculus L.) yang Dipajankan Monosodium Glutamat (MSG) Perlakuan Ulangan x ± SD

  1

  2

  3

  4

  5 K- 0,13 0,14 0,13 0,14 0,14 0,14 ± 0,01 K+ 0,14 0,13 0,13 0,15 0,14 0,14 ± 0,01 P1 0,09 0,08 0,11 0,11 0,09 0,10 ± 0,01 P2 0,14 0,13 0,15 0,12 0,14 0,14 ± 0,01 P3 0,13 0,14 0,15 0,14 0,14 0,14 ± 0,01

  Tests of Normality

  kelomp ok Kolmogorov-Smirnov

• .881

  5 .314 p1 .273 5 .200

• .852

  5 .201 p2 .237 5 .200

• .961

  5 .814 p3 .300 5 .161 .883 5 .325 p4 .221 5 .200

• .902

  5 .421

  a. Lilliefors Significance Correction *. This is a lower bound of the true significance.

  

Test of Homogeneity of Variance

Levene Statistic df1 df2 Sig.

  berat_testis Based on Mean 2.486

  5 24 .060 Based on Median .898

  5 24 .498 Based on Median and with adjusted df

  .898 5 16.777 .505 Based on trimmed mean 2.466

  5 24 .061

  5 .200

Kruskal-Wallis Test Ranks

  kelomp ok N Mean Rank berat_testis k-

  5

  16.40 k+

  5

  18.00 p1

  5

  3.00 p2

  5

  17.10 p3

  5

  19.80 p4

  5

  18.70 Total

  30

  a,b Test Statistics

  berat_testis Chi-Square 13.449 df

  5 Asymp. Sig. .020

  a. Kruskal Wallis Test

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis k-

  5

  5.20

  26.00 k+

  5

  5.80

  29.00 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U 11.000 Wilcoxon W 26.000 Z -.346 Asymp. Sig. (2-tailed) .729

  a

  Exact Sig. [2*(1-tailed Sig.)] .841 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis k-

  5

  8.00

  40.00 p1

  5

  3.00

  15.00 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U .000 Wilcoxon W 15.000 Z -2.668 Asymp. Sig. (2-tailed) .008

  a

  Exact Sig. [2*(1-tailed Sig.)] .008 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis k-

  5

  5.40

  27.00 p2

  5

  5.60

  28.00 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U 12.000 Wilcoxon W 27.000 Z -.113 Asymp. Sig. (2-tailed) .910

  a

  Exact Sig. [2*(1-tailed Sig.)] 1.000 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis k-

  5

  4.70

  23.50 p3

  5

  6.30

  31.50 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U 8.500 Wilcoxon W 23.500 Z -.956 Asymp. Sig. (2-tailed) .339

  a

  Exact Sig. [2*(1-tailed Sig.)] .421 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis k-

  5

  5.10

  25.50 p4

  5

  5.90

  29.50 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U 10.500 Wilcoxon W 25.500 Z -.438 Asymp. Sig. (2-tailed) .661

  a

  Exact Sig. [2*(1-tailed Sig.)] .690 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis k+

  5

  8.00

  40.00 p1

  5

  3.00

  15.00 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U .000 Wilcoxon W 15.000 Z -2.643 Asymp. Sig. (2-tailed) .008

  a

  Exact Sig. [2*(1-tailed Sig.)] .008 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis k+

  5

  5.70

  28.50 p2

  5

  5.30

  26.50 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U 11.500 Wilcoxon W 26.500 Z -.219 Asymp. Sig. (2-tailed) .827

  a

  Exact Sig. [2*(1-tailed Sig.)] .841 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis k+

  5

  5.10

  25.50 p3

  5

  5.90

  29.50 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U 10.500 Wilcoxon W 25.500 Z -.454 Asymp. Sig. (2-tailed) .650

  a

  Exact Sig. [2*(1-tailed Sig.)] .690 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis k+

  5

  5.40

  27.00 p4

  5

  5.60

  28.00 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U 12.000 Wilcoxon W 27.000 Z -.108 Asymp. Sig. (2-tailed) .914

  a

  Exact Sig. [2*(1-tailed Sig.)] 1.000 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis p1

  5

  3.00

  15.00 p2

  5

  8.00

  40.00 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U .000 Wilcoxon W 15.000 Z -2.635 Asymp. Sig. (2-tailed) .008

  a

  Exact Sig. [2*(1-tailed Sig.)] .008 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis p1

  5

  3.00

  15.00 p3

  5

  8.00

  40.00 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U .000 Wilcoxon W 15.000 Z -2.660 Asymp. Sig. (2-tailed) .008

  a

  Exact Sig. [2*(1-tailed Sig.)] .008 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis p1

  5

  3.00

  15.00 p4

  5

  8.00

  40.00 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U .000 Wilcoxon W 15.000 Z -2.635 Asymp. Sig. (2-tailed) .008

  a

  Exact Sig. [2*(1-tailed Sig.)] .008 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis p2

  5

  5.00

  25.00 p3

  5

  6.00

  30.00 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U 10.000 Wilcoxon W 25.000 Z -.561 Asymp. Sig. (2-tailed) .575

  a

  Exact Sig. [2*(1-tailed Sig.)] .690 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis p2

  5

  5.20

  26.00 p4

  5

  5.80

  29.00 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U 11.000 Wilcoxon W 26.000 Z -.323 Asymp. Sig. (2-tailed) .746

  a

  Exact Sig. [2*(1-tailed Sig.)] .841 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  Ranks

  kelomp ok N Mean Rank Sum of Ranks berat_testis p3

  5

  5.60

  28.00 p4

  5

  5.40

  27.00 Total

  10

  b Test Statistics

  berat_testis Mann-Whitney U 12.000 Wilcoxon W 27.000 Z -.110 Asymp. Sig. (2-tailed) .913

  a

  Exact Sig. [2*(1-tailed Sig.)] 1.000 a. Not corrected for ties.

  b. Grouping Variable: kelompok

  LAMPIRAN F. Data dan Analisis Statistik Volume Testis Mencit Rataan Volume Testis setelah Pemberian Vitamin C dan E pada Mencit (Mus musculus L.) yang Dipajankan Monosodium Glutamat (MSG) Perlakuan Ulangan x ± SD

  1

  2

  3

  4

  5 K(-) 121,71 117,28 95,7 135,64 102,16 114,49 ± 15,91 K(+) 121,71 124,42 135,64 95,7 95,7 114,63 ± 18,06 P1 73,27 88,65 83,73 73,27 68,04 77,39 ± 8,49 P2 102,16 68,04 105,51 103,04 121,71 100,09 ± 19,60 P3 117,28 135,64 101,32 117,28 103,41 114,98 ± 13,76

P4 103,41 117,28 135,64 91,82 121,71 113,97 ± 16,90 Hasil Uji Statistik Volume Testis Mencit

  

Tests of Normality

  KELOM POK Kolmogorov-Smirnov

  a

  Shapiro-Wilk Statistic df Sig. Statistic df Sig.

• .966
• .859
• .913
• .903
• .985

  5 .846 K+ .253 5 .200

  VOLUME_TESTIS K- .181 5 .200

  5 .484 P2 .342 5 .056 .866

  5 .250 P3 .234 5 .200

  5 .426 P4 .178 5 .200

  5 .958

  a. Lilliefors Significance Correction *. This is a lower bound of the true significance.

  

Test of Homogeneity of Variance

Levene Statistic df1 df2 Sig.

  VOLUME_TESTIS Based on Mean .569

  5 24 .723 Based on Median .300

  5 24 .908 Based on Median and with adjusted df

  .300 5 18.887 .907 Based on trimmed mean .550

  5 24 .736

  5 .226 P1 .286 5 .200

  P2 K- -14.40600 10.03512 1.000 -47.1043 18.2923 K+ -14.54200 10.03512 1.000 -47.2403 18.1563 P1 22.70000 10.03512 .495 -9.9983 55.3983 P3 -14.89400 10.03512 1.000 -47.5923 17.8043

  P2 14.40600 10.03512 1.000 -18.2923 47.1043 P3 -.48800 10.03512 1.000 -33.1863 32.2103 P4 .52600 10.03512 1.000 -32.1723 33.2243

  P4 -36.58000

  P2 -22.70000 10.03512 .495 -55.3983 9.9983 P3 -37.59400

  K+ -37.24200

  P1 K- -37.10600

  P2 14.54200 10.03512 1.000 -18.1563 47.2403 P3 -.35200 10.03512 1.000 -33.0503 32.3463 P4 .66200 10.03512 1.000 -32.0363 33.3603

  K+ K- .13600 10.03512 1.000 -32.5623 32.8343 P1 37.24200

  K- K+ -.13600 10.03512 1.000 -32.8343 32.5623 P1 37.10600

  ANOVA

  95% Confidence Interval Lower Bound Upper Bound

  Mean Difference (I-J) Std. Error Sig.

  (J) KELOM POK

  VOLUME_TESTIS Bonferroni (I) KELOM POK

  29 Multiple Comparisons

  VOLUME_TESTIS Sum of Squares df Mean Square F Sig. Between Groups 5721.774 5 1144.355 4.545 .005 Within Groups 6042.218 24 251.759 Total 11763.992

• 10.03512 .017 4.4077 69.8043
• 10.03512 .016 4.5437 69.9403
• 10.03512 .017 -69.8043 -4.4077
• 10.03512 .016 -69.9403 -4.5437
• 10.03512 .015 -70.2923 -4.8957
• 10.03512 .019 -69.2783 -3.8817

P4 -13.88000 10.03512 1.000 -46.5783 18.8183 P3 K- .48800 10.03512 1.000 -32.2103 33.1863

  K+ .35200 10.03512 1.000 -32.3463 33.0503 P1 37.59400

• 10.03512 .015 4.8957 70.2923

  P2 14.89400 10.03512 1.000 -17.8043 47.5923 P4 1.01400 10.03512 1.000 -31.6843 33.7123

  P4 K- -.52600 10.03512 1.000 -33.2243 32.1723 K+ -.66200 10.03512 1.000 -33.3603 32.0363 P1 36.58000

• 10.03512 .019 3.8817 69.2783

  P2 13.88000 10.03512 1.000 -18.8183 46.5783 P3 -1.01400 10.03512 1.000 -33.7123 31.6843 *. The mean difference is significant at the 0.05 level.

  

LAMPIRAN G. Data dan Analisis Statistik Diameter Tubulus Seminiferus Testis

Mencit

Rataan Diameter Tubulus Seminiferus Testis setelah Pemberian Vitamin C dan

E pada Mencit (Mus musculus L.) yang Dipajankan Monosodium Glutamat

(MSG) Perlakuan Ulangan x ± SD

  1

  2

  3

  4

  5 K- 245,2 173,51 243,66 230,88 218,4 222,31 ± 29,36

K+ 203,96 226,14 219,92 227,02 242,7 223,95 ± 13,98

P1 173,51 203,09 169,29 196,43 169,29 182,32 ± 16,18

  

P2 215,72 203,09 169,29 215,19 226,03 205,86 ± 22,00

P3 212,74 239,2 212,8 206,92 196,43 213,62 ± 15,78

P4 213,85 214,06 221,6 220,32 243,66 222,70 ± 12,24

Hasil Uji Statistik Diameter Tubulus Seminiferus Testis Mencit

  Tests of Normality

a

  Kolmogorov-Smirnov Shapiro-Wilk KELOM POK Statistic df Sig. Statistic df Sig.

• DIAMETER K- .246

  5 .200 .840 5 .165

• K+ .213

  5 .200 .965 5 .840 P1 .307 5 .139 .804

  5 .087

• P2 .264

  5 .200 .858 5 .221

• P3 .246

  5 .200 .944 5 .697 P4 .336 5 .067 .781

  5 .056

  a. Lilliefors Significance Correction *. This is a lower bound of the true significance.

  Test of Homogeneity of Variance Levene Statistic df1 df2 Sig.

  DIAMETER Based on Mean .898

  5 24 .499 Based on Median .412

  5 24 .836 P2 K- -304.28200 223.64228 1.000 -1032.9939 424.4299 K+ -333.70400 223.64228 1.000 -1062.4159 395.0079

  P2 -434.10600 223.64228 .961 -1162.8179 294.6059 P3 -591.07600 223.64228 .214 -1319.7879 137.6359 P4 -744.54000

  95% Confidence Interval Lower Bound Upper Bound

  K+ -767.81000

  P1 K- -738.38800

  P2 333.70400 223.64228 1.000 -395.0079 1062.4159 P3 176.73400 223.64228 1.000 -551.9779 905.4459 P4 23.27000 223.64228 1.000 -705.4419 751.9819

  K+ K- 29.42200 223.64228 1.000 -699.2899 758.1339 P1 767.81000

  P2 304.28200 223.64228 1.000 -424.4299 1032.9939 P3 147.31200 223.64228 1.000 -581.3999 876.0239 P4 -6.15200 223.64228 1.000 -734.8639 722.5599

  K- K+ -29.42200 223.64228 1.000 -758.1339 699.2899 P1 738.38800

  Mean Difference (I-J) Std. Error Sig.

  Based on Median and with adjusted df .412 5 16.405 .834

  (J) KELOM POK

  29 Multiple Comparisons DIAMETER Bonferroni (I) KELOM POK

  DIAMETER Sum of Squares df Mean Square F Sig. Between Groups 2191495.311 5 438299.062 3.505 .016 Within Groups 3000952.077 24 125039.670 Total 5192447.388

  ANOVA

  5 24 .532

  Based on trimmed mean .844

• 223.64228 .045 9.6761 1467.0999
• 223.64228 .033 39.0981 1496.5219
• 223.64228 .045 -1467.0999 -9.6761
• 223.64228 .033 -1496.5219 -39.0981
• 223.64228 .042 -1473.2519 -15.8281

P3 K- -147.31200 223.64228 1.000 -876.0239 581.3999 K+ -176.73400 223.64228 1.000 -905.4459 551.9779 P1 591.07600 223.64228 .214 -137.6359 1319.7879 P2 156.97000 223.64228 1.000 -571.7419 885.6819 P4 -153.46400 223.64228 1.000 -882.1759 575.2479

  P4 K- 6.15200 223.64228 1.000 -722.5599 734.8639 K+ -23.27000 223.64228 1.000 -751.9819 705.4419 P1 744.54000

  P1 434.10600 223.64228 .961 -294.6059 1162.8179 P3 -156.97000 223.64228 1.000 -885.6819 571.7419 P4 -310.43400 223.64228 1.000 -1039.1459 418.2779

• 223.64228 .042 15.8281 1473.2519

  P2 310.43400 223.64228 1.000 -418.2779 1039.1459 P3 153.46400 223.64228 1.000 -575.2479 882.1759 *. The mean difference is significant at the 0.05 level. a. Lilliefors Significance Correction *. This is a lower bound of the true significance.

  5 .553

  5

  44

  41

  1 76 143

  6,95 37,6 ± 10,55 19,6 ± 4,72 23,4 ± 6,83 38,8 ± 10,21 37,4 ± 4,83 Spermatid

  43 x ± SD 38,6 ±

  33

  14

  23

  

52

  46

  39

  71

  39

  24

  22

  

38

  34

  4

  39

  43

  33

  15

  91

  2

  43

  43

  5 .619 p4 .187 5 .200

  5 .959 p3 .193 5 .200

  5 .529 p1 .311 5 .129 .906 5 .445 p2 .188 5 .200

  5 .622 k+ .225 5 .200

  Shapiro-Wilk Statistic df Sig. Statistic df Sig. spermatogonium k- .195 5 .200

  a

  kelomp ok Kolmogorov-Smirnov

  42,4 ± 4,22 45,6 ± 7,37 93,8 ± 28,84 96,6 ± 22,88 Hasil Uji Statistik Jumlah Sel Spermatogenik Testis Mencit

Tests of Normality

  22,86

103,6±

26,35

  39 93 s128 x ± SD 101,6±

  

72

  98

  47 56 118 111 5 122

  91 4 128 112

  45 50 119

  

99

  84

  3

  82

  48

  39

  36

  

92

  

26

  3

  Lampiran H. Data dan Analisis Statistik Jumlah Sel Spermatogenik Testis Mencit Sel yang Diamati Ulangan K (-) K(+) P1 P2 P3 P4 Spermatogonium

  34

  4

  49

  47

  25

  26

  

32

  32

  3

  40

  39

  14

  

45

  

46

  41

  2

  54

  25

  17

  34

  

36

  30

  1

  43

  26

  36

  

43

  53

  24

  14

  

29

  41

  2

  30

  26

  21

  24

  29

  24

  1

  5,59 39,4 ± 5,98 25,8 ± 7,36 25,8 ± 6,30 37,4 ± 9,32 48,6 ± 5,59 Sp. Primer

  53 x ± SD 36,4 ±

  31

  29

  29

  

38

  36

  5

  47

  45

• .934
• .920
• .985
• .933
• .924

  

Test of Homogeneity of Variance

Levene Statistic df1 df2 Sig.

  spermatogonium Based on Mean .589

  5 24 .708 Based on Median .407

  5 24 .839 Based on Median and with adjusted df

  .407 5 19.977 .838 Based on trimmed mean .572

  5 24 .721

  ANOVA

  Spermatogonium Sum of Squares df Mean Square F Sig. Between Groups 1896.967 5 379.393 8.156 .000 Within Groups 1116.400 24 46.517 Total 3013.367

  29 Multiple Comparisons Spermatogonium Bonferroni (I) kelomp ok

  (J) kelomp ok

  Mean Difference (I-J) Std. Error Sig.

  95% Confidence Interval Lower Bound Upper Bound k- k+ -3.00000 4.31354 1.000 -17.0552 11.0552 p1 10.60000 4.31354 .324 -3.4552 24.6552 p2 10.60000 4.31354 .324 -3.4552 24.6552 p3 -1.00000 4.31354 1.000 -15.0552 13.0552 p4 -12.20000 4.31354 .139 -26.2552 1.8552 k+ k- 3.00000 4.31354 1.000 -11.0552 17.0552 p1 13.60000 4.31354 .065 -.4552 27.6552 p2 13.60000 4.31354 .065 -.4552 27.6552 p3 2.00000 4.31354 1.000 -12.0552 16.0552 p4 -9.20000 4.31354 .651 -23.2552 4.8552 p1 k- -10.60000 4.31354 .324 -24.6552 3.4552 k+ -13.60000 4.31354 .065 -27.6552 .4552 p2 .00000 4.31354 1.000 -14.0552 14.0552 p3 -11.60000 4.31354 .192 -25.6552 2.4552 p4 -22.80000

• 4.31354 .000 -36.8552 -8.7448 p2 k- -10.60000 4.31354 .324 -24.6552 3.4552 k+ -13.60000 4.31354 .065 -27.6552 .4552 p1 .00000 4.31354 1.000 -14.0552 14.0552 p3 -11.60000 4.31354 .192 -25.6552 2.4552 p4 -22.80000
• 4.31354 .000 -36.8552 -8.7448 p3 k- 1.00000 4.31354 1.000 -13.0552 15.0552 k+ -2.00000 4.31354 1.000 -16.0552 12.0552 p1 11.60000 4.31354 .192 -2.4552 25.6552 p2 11.60000 4.31354 .192 -2.4552 25.6552 p4 -11.20000 4.31354 .237 -25.2552 2.8552 p4 k- 12.20000 4.31354 .139 -1.8552 26.2552 k+ 9.20000 4.31354 .651 -4.8552 23.2552 p1 22.80000
• 4.31354 .000 8.7448 36.8552 p2 22.80000
• 4.31354 .000 8.7448 36.8552 p3 11.20000 4.31354 .237 -2.8552 25.2552

  Tests of Normality a

  Kolmogorov-Smirnov Shapiro-Wilk kelom pok Statistic df Sig. Statistic df Sig.

• spermatosit_primer k- .235

  5 .200 .939 5 .661

• k+ .193

  5 .200 .959 5 .799 p1 .294 5 .181 .825 5 .127

• p2 .253

  5 .200 .954 5 .769

• p3 .140

  5 .200 .994 5 .993

• p4 .230

  5 .200 .941 5 .672

  a. Lilliefors Significance Correction *. This is a lower bound of the true significance.

  Test of Homogeneity of Variance

  Levene Statistic df1 df2 Sig. spermatosit_primer Based on Mean 1.008

  5 24 .434 Based on Median .854

  5 24 .526 Based on Median and .854 5 21.252 .528 with adjusted df Based on trimmed mean 1.029

  5 24 .423

  ANOVA

  spermatosit_primer Sum of Squares Df Mean Square F Sig. Between Groups 1899.067 5 379.813 6.400 .001 Within Groups 1424.400 24 59.350 Total 3323.467

  29

  Multiple Comparisons

  spermatosit_primer Bonferroni (I) kelomp ok

  (J) kelomp ok

  Mean Difference (I-J) Std. Error Sig.

  95% Confidence Interval Lower Bound Upper Bound k- k+ 1.00000 4.87237 1.000 -14.8760 16.8760 p1 19.00000

• 4.87237 .010 3.1240 34.8760 p2 15.40000 4.87237 .063 -.4760 31.2760 p3 -.20000 4.87237 1.000 -16.0760 15.6760 p4 1.20000 4.87237 1.000 -14.6760 17.0760 k+ k- -1.00000 4.87237 1.000 -16.8760 14.8760 p1 18.00000
• 4.87237 .017 2.1240 33.8760 p2 14.40000 4.87237 .103 -1.4760 30.2760 p3 -1.20000 4.87237 1.000 -17.0760 14.6760 p4 .20000 4.87237 1.000 -15.6760 16.0760 p1 k- -19.00000
• 4.87237 .010 -34.8760 -3.1240 k+ -18.00000
• 4.87237 .017 -33.8760 -2.1240 p2 -3.60000 4.87237 1.000 -19.4760 12.2760 p3 -19.20000
• 4.87237 .009 -35.0760 -3.3240 p4 -17.80000
• 4.87237 .019 -33.6760 -1.9240 p2 k- -15.40000 4.87237 .063 -31.2760 .4760 k+ -14.40000 4.87237 .103 -30.2760 1.4760 p1 3.60000 4.87237 1.000 -12.2760 19.4760 p3 -15.60000 4.87237 .057 -31.4760 .2760 p4 -14.20000 4.87237 .114 -30.0760 1.6760 p3 k- .20000 4.87237 1.000 -15.6760 16.0760 k+ 1.20000 4.87237 1.000 -14.6760 17.0760 p1 19.20000
• 4.87237 .009 3.3240 35.0760 p2 15.60000 4.87237 .057 -.2760 31.4760 p4 1.40000 4.87237 1.000 -14.4760 17.2760 p4 k- -1.20000 4.87237 1.000 -17.0760 14.6760 k+ -.20000 4.87237 1.000 -16.0760 15.6760 p1 17.80000
• 4.87237 .019 1.9240 33.6760

p2 14.20000 4.87237 .114 -1.6760 30.0760 p3 -1.40000 4.87237 1.000 -17.2760 14.4760

• . The mean difference is significant at the 0.05 level.

  Tests of Normality

a

  Kolmogorov-Smirnov Shapiro-Wilk kelomp ok Statistic df Sig. Statistic df Sig.

• spermatid k- .214

  5 .200 .915 5 .496

• k+ .175

  5 .200 .974 5 .902

• p1 .170

  5 .200 .962 5 .822

• p2 .215

  5 .200 .896 5 .391

• p3 .261

  5 .200 .867 5 .254

• p4 .197

  5 .200 .962 5 .825

  a. Lilliefors Significance Correction *. This is a lower bound of the true significance.

  Test of Homogeneity of Variance Levene Statistic df1 df2 Sig.

  spermatid Based on Mean 2.168

  5 24 .092 Based on Median 1.578

  5 24 .204 Based on Median and with 1.578 5 14.940 .226 adjusted df Based on trimmed mean 2.188

  5 24 .089

  ANOVA

  Spermatid Sum of Squares df Mean Square F Sig. Between Groups 17849.900 5 3569.980 9.545 .000 Within Groups 8976.800 24 374.033 Total 26826.700

  29

  Multiple Comparisons

  Spermatid Bonferroni (I) kelomp ok

  (J) kelomp ok

  Mean Difference (I-J) Std. Error Sig.

  95% Confidence Interval Lower Bound Upper Bound k- k+ -2.00000 13.27705 1.000 -45.2617 41.2617 p1 59.20000

• 13.27705 .002 15.9383 102.4617 p2 56.00000
• 13.27705 .005 12.7383 99.2617 p3 7.80000 13.27705 1.000 -35.4617 51.0617 p4 5.00000 13.27705 1.000 -38.2617 48.2617 k+ k- 2.00000 13.27705 1.000 -41.2617 45.2617 p1 61.20000
• 13.27705 .002 17.9383 104.4617 p2 58.00000
• 13.27705 .003 14.7383 101.2617 p3 9.80000 13.27705 1.000 -33.4617 53.0617 p4 7.00000 13.27705 1.000 -36.2617 50.2617 p1 k- -59.20000
• 13.27705 .002 -102.4617 -15.9383 k+ -61.20000
• 13.27705 .002 -104.4617 -17.9383 p2 -3.20000 13.27705 1.000 -46.4617 40.0617 p3 -51.40000
• 13.27705 .011 -94.6617 -8.1383 p4 -54.20000
• 13.27705 .006 -97.4617 -10.9383 p2 k- -56.00000
• 13.27705 .005 -99.2617 -12.7383 k+ -58.00000
• 13.27705 .003 -101.2617 -14.7383 p1 3.20000 13.27705 1.000 -40.0617 46.4617 p3 -48.20000
• 13.27705 .020 -91.4617 -4.9383 p4 -51.00000
• 13.27705 .012 -94.2617 -7.7383 p3 k- -7.80000 13.27705 1.000 -51.0617 35.4617 k+ -9.80000 13.27705 1.000 -53.0617 33.4617 p1 51.40000
• 13.27705 .011 8.1383 94.6617 p2 48.20000
• 13.27705 .020 4.9383 91.4617 p4 -2.80000 13.27705 1.000 -46.0617 40.4617 p4 k- -5.00000 13.27705 1.000 -48.2617 38.2617 k+ -7.00000 13.27705 1.000 -50.2617 36.2617 p1 54.20000
• 13.27705 .006 10.9383 97.4617
• 13.27705 .012 7.7383 94.2617 p3 2.80000 13.27705 1.000 -40.4617 46.0617 *. The mean difference is significant at the 0.05 level.

  p2 51.00000

  Lampiran I. Rekomendasi Persetujuan Etik Penelitian Kesehatan