Efek Hipoglikemik Polisakarida Larut Air (PLA) Umbi Talas Kimpul (Xanthosoma sagittifolium) Terhadap Glukosa Darah Tikus Wistar
Lampiran 1. Kurva Standard Glukosa, Fenol-Sulfat, DNS
Absorbansi
Kurva standar glukosa
0,749
0,8
0,7
0,6
0,5
0,4
0,3
0,2
0,1
0
0,598
0,423
y = 0,087x - 0,115
R² = 0,996
0,209
0,068
0
2
4
6
8
10
12
Konsentrasi (µg/ml)
Kurva standar glukosa untuk penentuan kadar glukosa dengan metode Nelson-somogyi
Kurva standard fenol-sulfat
0,626
0,7
0,532
Absorbansi
0,6
0,437
0,5
0,341
0,4
0,3
y = 0,010x + 0,013
R² = 0,996
0,208
0,2
0,114
0,1
0
0
10
20
30
40
50
60
70
Konsentrasi (mg/ml)
Kurva standard glukosa untuk penentuan total gula dengan metode fenol-sulfat
Universitas Sumatera Utara
Absorbansi
Kurva standard DNS
0,851
0,9
0,8
0,7
0,6
0,5
0,4
0,3
0,2
0,1
0
0,572
0,394
0,151
0
0,05
0,1
y = 4,556x - 0,305
R² = 0,993
0,15
0,2
0,25
0,3
Konsentrasi (mg/ml)
Kurva standard glukosa untuk gula pereduksi dengan metode DNS
Universitas Sumatera Utara
Lampiran 2. Analisis sidik ragam rendemen PLA dari berbagai metode
ekstraksi
Descriptives
Rendemen
Ektraksi Air
Ekstraksi Papain
Ekstraksi Ragi
tempe
Total
N
Mean
Std.
Deviation
Std.
Error
3
3
13.5333
13.8233
.35642
.15567
95% Confidence Minim Maxi
Interval for Mean
um
mum
Lower
Upper
Bound Bound
.20578 12.6479 14.4187 13.24 13.93
.08988 13.4366 14.2100 13.66 13.97
3
12.3533
.23861
.13776 11.7606 12.9461
12.08
12.52
9
13.2367
.71185
.23728 12.6895 13.7838
12.08
13.97
ANOVA
Rendemen
Between Groups
Within Groups
Total
Sum of
Squares
3.637
.416
4.054
df
Mean Square
2
6
8
1.819
.069
F
26.206
Sig.
.001
Rendemen PLA Talas Kimpul
Rendemen
N
Subset for alpha = 0.05
1
2
Ekstraksi Ragi tempe
3
12.3533
Ektraksi Air
3
13.5333
Duncana
Ekstraksi Papain
3
13.8233
Sig.
1.000
.226
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Universitas Sumatera Utara
Lampiran 3. Analisis Sidik Ragam Kadar Air PLA dari berbagai metode
ekstraksi
Descriptives
Kadar air PLA talas kimpul
N Mean
Ekstraksi dengan air
Ekstraksi dengan
papain
Ekstraksi dengan ragi
tempe
Total
Std.
Deviation
Std.
Error
3
3.7100
95% Confidence Minim Maxim
Interval for Mean um
um
Lower Upper
Bound Bound
.01000 .00577 3.6852 3.7348
3.70
3.72
3
2.4800
.02000 .01155
2.4303 2.5297
2.46
2.50
3
2.8633
.14844 .08570
2.4946 3.2321
2.70
2.99
9
3.0178
.55020 .18340
2.5949 3.4407
2.46
3.72
ANOVA
Kadar Air PLA talas kimpul
Sum of Squares
Between Groups
2.377
Within Groups
.045
Total
2.422
df
2
6
8
Mean Square
1.188
.008
F
158.212
Sig.
.000
Kadar Air PLA Talas Kimpul
Kadar Air
N
Subset for alpha = 0.05
1
2
3
Ekstraksi dengan papain
3
2.4800
Ekstraksi dengan ragi tempe
3
2.8633
Duncana
Ekstraksi dengan air
3
3.7100
Sig.
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Universitas Sumatera Utara
Lampiran 4. Analisis sidik ragam kadar Serat PLA talas kimpul dari berbagai
metode ekstraksi
Descriptives
Persentase kadar serat PLA
Ekstraksi dengan air
Ekstraksi dengan
papain
Ekstraksi dengan ragi
tempe
Total
N
Mean
Std.
Deviation
Std.
Error
95% Confidence Mini Maxim
Interval for
mum
um
Mean
Lower Upper
Bound Bound
3
.4800
.01000
.00577
.4552
.5048
.47
.49
3
.4400
.01732
.01000
.3970
.4830
.42
.45
3
.3300
.01000
.00577
.3052
.3548
.32
.34
9
.4167
.06819
.02273
.3643
.4691
.32
.49
ANOVA
Persentase kadar serat PLA
Sum of
Squares
Between Groups
.036
Within Groups
.001
Total
.037
df
2
6
8
Mean
Square
.018
.000
F
108.600
Sig.
.000
Persentase kadar serat PLA
SeratKasar
N
Subset for alpha = 0.05
1
2
3
Ekstraksi dengan ragi tempe
3
.3300
Ekstraksi dengan papain
3
.4400
Duncana
Ekstraksi dengan air
3
.4800
Sig.
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Universitas Sumatera Utara
Lampiran 5. Analisis sidik ragam kadar pati PLA talas kimpul dari berbagai
metode ekstraksi
Oneway
Descriptives
Kadar Pati
N
Ekstraksi Air
Ekstraksi Papain
Ekstraksi Ragi Tempe
Total
Mean
3
3
3
9
Std.
Deviation
.1379
.1353
.1309
.1347
Std.
Error
.00145
.00040
.00199
.00331
.00084
.00023
.00115
.00110
95% Confidence Mini Maxi
Interval for
mum mum
Mean
Lower Upper
Bound Bound
.1343 .1415
.14
.14
.1343 .1363
.14
.14
.1259 .1358
.13
.13
.1321 .1372
.13
.14
ANOVA
Kadar Pati
Between Groups
Within Groups
Total
Sum of
Squares
.000
.000
.000
df
Mean Square
2
6
8
.000
.000
F
18.234
Sig.
.003
Kadar Pati
Perlakuan
N
Ekstraksi Ragi Tempe
Ekstraksi Papain
Duncana
Ekstraksi Air
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Subset for alpha = 0.05
1
2
3
.1309
3
.1353
3
.1379
1.000
.074
Universitas Sumatera Utara
Lampiran 6. Analisis sidik ragam kadar glukosa PLA talas kimpul dari
berbagai metode ekstraksi
Descriptives
Kadar glukosa
N
Ekstraksi air
Ekstraksi papain
Ekstraksi Ragi Tempe
Total
3
3
3
9
Mean
Std.
Deviation
.0529
.0537
.0541
.0536
Std.
Error
.00228
.00141
.00083
.00149
.00132
.00081
.00048
.00050
95% Confidence Mini Maxi
Interval for
mum mum
Mean
Lower Upper
Bound Bound
.0473 .0586
.05
.05
.0502 .0572
.05
.06
.0520 .0561
.05
.06
.0524 .0547
.05
.06
ANOVA
Kadar glukosa
Between Groups
Within Groups
Total
Sum of
Squares
.000
.000
.000
df
Mean Square
2
6
8
.000
.000
F
.382
Sig.
.698
Kadar Glukosa
Perlakuan
Ekstraksi air
Ekstraksi papain
Duncana
Ekstraksi Ragi Tempe
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
N
Subset for alpha
= 0.05
1
3
.0529
3
.0537
3
.0541
.439
Universitas Sumatera Utara
Lampiran 7. Analisis sidik ragam kadar gula pereduksi PLA talas kimpul dari
berbagai metode ekstraksi
Oneway
Descriptives
Gula reduksi
N
Ekstraksi Air
Ekstraksi Papain
Ekstraksi Ragi Tempe
Total
3
3
3
9
Mean
Std.
Deviation
.08047
.08243
.08377
.08222
Std.
Error
.000737
.001850
.001692
.001943
.000426
.001068
.000977
.000648
95% Confidence Mini Maxi
Interval for
mum mum
Mean
Lower Upper
Bound Bound
.07864 .08230 .080 .081
.07784 .08703 .081 .084
.07956 .08797 .082 .085
.08073 .08372 .080 .085
ANOVA
Gula reduksi
Between Groups
Within Groups
Total
Sum of
Squares
.000
.000
.000
df
Mean Square
2
6
8
.000
.000
F
3.632
Sig.
.093
Gula reduksi
Perlakuan
N
Ekstraksi Air
Ekstraksi Papain
Duncana
Ekstraksi Ragi Tempe
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Subset for alpha = 0.05
1
2
3
.08047
3
.08243
.08243
3
.08377
.162
.321
Universitas Sumatera Utara
Lampiran 8. Analisis sidik ragam total gula PLA talas kimpul dari berbagai
metode ekstraksi
Descriptives
Total gula
N
Ekstraksi air
Ekstraksi papain
Ekstraksi ragi tempe
Total
3
3
3
9
Mean
Std.
Deviation
1.7980
1.7846
1.7711
1.7846
Std.
Error
.28782
.06062
.08313
.15327
.16617
.03500
.04800
.05109
95% Confidence Mini Maxi
Interval for
mum mum
Mean
Lower Upper
Bound Bound
1.0830 2.5130
1.56
2.12
1.6340 1.9352
1.72
1.84
1.5646 1.9776
1.68
1.84
1.6668 1.9024
1.56
2.12
ANOVA
Total gula
Between Groups
Within Groups
Total
Sum of
Squares
.001
.187
.188
df
Mean Square
2
6
8
.001
.031
F
.017
Sig.
.983
Total gula
Perlakuan
Ekstraksi ragi tempe
Ekstraksi papain
Duncana
Ekstraksi air
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
N
Subset for alpha
= 0.05
1
3
1.7711
3
1.7846
3
1.7980
.862
Universitas Sumatera Utara
Lampiran 9. Analisis sidik ragam daya larut PLA talas kimpul dari berbagai
metode ekstraksi
Descriptives
Daya larut PLA
Ekstraksi dengan air
Ekstraksi dengan
papain
Ekstraksi dengan
ragi tempe
Total
N
Mean
Std.
Deviation
Std.
Error
3
76.8400
.54342 .31374
95% Confidence
Mini Maxi
Interval for Mean mum mum
Lower
Upper
Bound
Bound
75.4901 78.1899 76.36 77.43
3
80.6800
.17776 .10263
80.2384
81.1216 80.48 80.82
3
82.5200
.04359 .02517
82.4117
82.6283 82.47 82.55
9
80.0133
2.52614 .84205
78.0716
81.9551 76.36 82.55
ANOVA
Daya larut PLA
Between Groups
Within Groups
Total
Perlakuan
Sum of
Squares
50.394
.658
51.051
df
Mean Square
2
6
8
25.197
.110
F
229.898
Sig.
.000
Daya larut PLA
N
Subset for alpha = 0.05
1
2
3
Ekstraksi dengan air
3
76.8400
Ekstraksi dengan papain
3
80.6800
Duncana
Ekstraksi dengan ragi tempe
3
82.5200
Sig.
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Universitas Sumatera Utara
Lampiran 10. Analisis sidik ragam viskositas PLA talas kimpul dari berbagai
metode ekstraksi
Descriptives
Viskositas PLA
Ekstraksi dengan
air
Ekstraksi dengan
papain
Ekstraksi dengan
ragi tempe
Total
N
Mean
Std.
Std. Error
Deviation
95% Confidence Minim Maxi
Interval for Mean
um
mum
Lower
Upper
Bound
Bound
3
1.0267
.04163
.02404
.9232
1.1301
.98
1.06
3
.9700
.01000
.00577
.9452
.9948
.96
.98
3
.9667
.00577
.00333
.9523
.9810
.96
.97
9
.9878
.03632
.01211
.9599
1.0157
.96
1.06
ANOVA
Viskositas PLA
Between Groups
Within Groups
Total
Sum of
Squares
.007
.004
.011
df
Mean Square
2
6
8
.003
.001
F
5.482
Sig.
.044
Viskositas PLA
Viskositas (cP)
N
Ekstraksi dengan ragi tempe
3
Ekstraksi dengan papain
3
Duncana
Ekstraksi dengan air
3
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Subset for alpha = 0.05
1
2
.9667
.9700
1.0267
.875
1.000
Universitas Sumatera Utara
Lampiran 11. Analisis sidik ragam dextrose equivalen PLA talas kimpul dari
berbagai metode ekstraksi
Descriptives
Dextrose equivalen
N
Ekstraksi Air
Ekstraksi Papain
Ekstraksi Ragi Tempe
Total
3
3
3
9
Mean
Std.
Deviation
4.5483
4.6227
4.7374
4.6362
Std.
Error
.68818
.16025
.27979
.38884
95% Confidence Mini Maxi
Interval for
mum mum
Mean
Lower Upper
Bound Bound
.39732
.09252
.16154
.12961
2.8388
4.2247
4.0424
4.3373
6.2578
5.0208
5.4325
4.9350
3.79
4.48
4.46
3.79
5.12
4.80
5.02
5.12
ANOVA
Dextrose Equivalen
Between Groups
Within Groups
Total
Sum of
Squares
.054
1.155
1.210
df
Mean Square
2
6
8
Dextrose Equivalen
Perlakuan
Ekstraksi Air
Ekstraksi Papain
Duncana
Ekstraksi Ragi Tempe
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
F
.027
.193
Sig.
.141
N
3
3
3
.871
Subset for
alpha = 0.05
1
4.5483
4.6227
4.7374
.627
Universitas Sumatera Utara
Lampiran 12. Analisis sidik ragam derajat polimerisasi PLA talas kimpul dari
berbagai metode ekstraksi
Descriptives
Derajat polimerisasi
N
Ekstraksi air
Ekstraksi Papain
Ekstraksi Ragi
tempe
Total
Mean
3
3
Std.
Std. Error 95% Confidence Minim Maxi
Deviation
Interval for Mean
um
mum
Lower
Upper
Bound Bound
22.3495
3.60892 2.08361 13.3845 31.3146 19.52 26.41
21.6494
.74248
.42867 19.8050 23.4938 20.84 22.30
3
21.1578
1.25210
.72290 18.0474 24.2682
19.93
22.44
9
21.7189
2.01366
.67122 20.1711 23.2667
19.52
26.41
ANOVA
Derajat polimerisasi
Between Groups
Within Groups
Total
Sum of
Squares
2.152
30.287
32.439
df
Mean Square
2
6
8
1.076
5.048
F
Sig.
.213
.814
Derajat Polimerisasi
Perlakuan
Ekstraksi Ragi tempe
Ekstraksi Papain
Duncana
Ekstraksi air
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
N
3
3
3
Subset for
alpha = 0.05
1
21.1578
21.6494
22.3495
.552
Universitas Sumatera Utara
Lampiran 13. Hasil Analisa Sidik Ragam Kadar Glukosa Darah Tikus
Perminggu
Descriptives
Rerata Kadar Glukosa Darah perminggu
Normal
Negatif
Positif
Perlakuan
Total
N
Mean
5
5
5
5
20
68.5370
217.4220
156.3782
158.8000
150.2843
Std.
Deviation
Std.
Error
1.1070
.4951
1.0437
.4667
51.0784 22.8429
46.2869 20.7001
63.0430 14.0968
95% Confidence
Interval for Mean
Lower
Upper
Bound
Bound
67.1623
69.9116
216.1260 218.7180
92.9559 219.8005
101.3272 216.2728
120.7793 179.7893
Mini
mum
Maxi
mum
67.15
216.25
91.83
101.71
67.15
69.96
218.82
220.29
218.62
220.29
ANOVA
Kadar Glukosa Darah
Between Groups
Within Groups
Total
Sum of Squares
56498.756
19015.218
75513.973
df
3
16
19
Mean Square
18832.919
1188.451
F
15.847
Sig.
.000
Rerata Kadar Glukosa Darah Tiap Minggu
Perlakuan
N
Subset for alpha = 0.05
1
2
3
Normal
5
68.5370
Positif
5
156.3782
a
Duncan
Perlakuan
5
158.8000
Negatif
5
217.4220
Sig.
1.000
.913
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 14. Hasil Sidik Ragam Glucose Tolerance Test
Descriptives
Glucose Tolerance Test
menit ke 0
menit ke 30
menit ke 60
menit ke 90
menit ke 120
Total
N
Mean
3
3
3
3
3
15
112.5000
129.5333
101.9100
97.2067
75.0000
103.2300
Std.
Deviation
5.10565
5.62932
4.58429
2.35239
4.52019
18.98823
Std.
Error
95% Confidence
Interval for Mean
Lower
Upper
Bound
Bound
Mini
mum
Maxi
mum
2.94775 99.8169 125.1831 109.19
3.25009 115.5493 143.5173 126.10
2.64674 90.5220 113.2980 98.64
1.35815 91.3630 103.0503 94.64
2.60973 63.7712 86.2288 71.69
4.90274 92.7147 113.7453 71.69
118.38
136.03
107.15
99.26
80.15
136.03
ANOVA
Glucose Tolerance Test
Between Groups
Within Groups
Total
Sum of
Squares
4838.262
209.477
5047.739
df
4
10
14
Mean Square
1209.566
20.948
F
Sig.
57.742
Glucose Tolerance Test
Perlakuan
N
Subset for alpha = 0.01
1
2
3
menit ke 120
3
75.0000
menit ke 90
3
97.2067
menit ke 60
3
101.9100 101.9100
Duncana
menit ke 0
3
112.5000
menit ke 30
3
Sig.
1.000
.237
.018
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
.000
4
129.5333
1.000
Universitas Sumatera Utara
Lampiran 15. Hasil Sidik Ragam Berat Badan Tikus
Descriptives
Berat Badan Tikus
Normal
Negatif
Positif
Perlakuan
Total
N
Mean
6
6
6
6
24
220.666
176.333
200.000
200.833
199.458
Std.
Deviation
1.0328
6.3770
6.4187
6.6156
16.8806
Std.
Error
.4216
2.6034
2.6204
2.7008
3.4457
95% Confidence Interval
for Mean
Lower
Upper
Bound
Bound
219.5828
221.7505
169.6410
183.0256
193.2640
206.7360
193.8906
207.7760
192.3303
206.5864
Mini
mum
Maxi
mum
219.00
169.00
188.00
191.00
169.00
222.00
187.00
206.00
208.00
222.00
ANOVA
Berat Badan Tikus
Between Groups
Within Groups
Total
Sum of Squares
5920.458
633.500
6553.958
df
3
20
23
Mean Square
1973.486
31.675
F
62.304
Sig.
.000
Berat Badan Tikus
Perlakuan
N
Subset for alpha = 0.01
1
2
3
Negatif
6
176.3333
Positif
6
200.0000
a
Duncan
Perlakuan
6
200.8333
Normal
6
220.6667
Sig.
1.000
.800
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6.000.
Universitas Sumatera Utara
Lampiran 16. Dokumentasi Kegiatan Penelitian
Penimbangan berat badan tikus
Nicotinamide dan Streptozotocin
Pengambilan darah secara retro orbital plexus
PLA setelah dilarutkan dengan aquades
Proses analisa in situ pada shaker water bath (pada suhu 37 0 C)
Universitas Sumatera Utara
Injeksi Nicotinamide dan streptozotocin
Kandang Percobaan
Hasil sentrifugasi digesta
Darah tikus setelah oksidasi
Penampakan secara visual tikus
P1, P2, P3, P4
Universitas Sumatera Utara
Lampiran 17. Kromatogram Gas Chromatography Analisa SCFA
Kromatogram Standar SCFA (Short Chain Fatty Acid)
Kromatogram tikus kontrol normal 1
Universitas Sumatera Utara
Kromatogram tikus kontrol normal 2
Kromatogram tikus kontrol normal 2
Universitas Sumatera Utara
Kromatogram tikus kelompok negatif 1
Kromatogram tikus kelompok negatif 2
Universitas Sumatera Utara
Kromatogram tikus kelompok negatif 3
Kromatogram tikus kelompok positif 1
Universitas Sumatera Utara
Kromatogram tikus kelompok positif 2
Kromatogram tikus kelompok positif 3
Universitas Sumatera Utara
Kromatogram tikus kelompok perlakuan 1
Kromatogram tikus kelompok perlakuan 2
Universitas Sumatera Utara
Kromatogram tikus kelompok perlakuan 3
Universitas Sumatera Utara
Absorbansi
Kurva standar glukosa
0,749
0,8
0,7
0,6
0,5
0,4
0,3
0,2
0,1
0
0,598
0,423
y = 0,087x - 0,115
R² = 0,996
0,209
0,068
0
2
4
6
8
10
12
Konsentrasi (µg/ml)
Kurva standar glukosa untuk penentuan kadar glukosa dengan metode Nelson-somogyi
Kurva standard fenol-sulfat
0,626
0,7
0,532
Absorbansi
0,6
0,437
0,5
0,341
0,4
0,3
y = 0,010x + 0,013
R² = 0,996
0,208
0,2
0,114
0,1
0
0
10
20
30
40
50
60
70
Konsentrasi (mg/ml)
Kurva standard glukosa untuk penentuan total gula dengan metode fenol-sulfat
Universitas Sumatera Utara
Absorbansi
Kurva standard DNS
0,851
0,9
0,8
0,7
0,6
0,5
0,4
0,3
0,2
0,1
0
0,572
0,394
0,151
0
0,05
0,1
y = 4,556x - 0,305
R² = 0,993
0,15
0,2
0,25
0,3
Konsentrasi (mg/ml)
Kurva standard glukosa untuk gula pereduksi dengan metode DNS
Universitas Sumatera Utara
Lampiran 2. Analisis sidik ragam rendemen PLA dari berbagai metode
ekstraksi
Descriptives
Rendemen
Ektraksi Air
Ekstraksi Papain
Ekstraksi Ragi
tempe
Total
N
Mean
Std.
Deviation
Std.
Error
3
3
13.5333
13.8233
.35642
.15567
95% Confidence Minim Maxi
Interval for Mean
um
mum
Lower
Upper
Bound Bound
.20578 12.6479 14.4187 13.24 13.93
.08988 13.4366 14.2100 13.66 13.97
3
12.3533
.23861
.13776 11.7606 12.9461
12.08
12.52
9
13.2367
.71185
.23728 12.6895 13.7838
12.08
13.97
ANOVA
Rendemen
Between Groups
Within Groups
Total
Sum of
Squares
3.637
.416
4.054
df
Mean Square
2
6
8
1.819
.069
F
26.206
Sig.
.001
Rendemen PLA Talas Kimpul
Rendemen
N
Subset for alpha = 0.05
1
2
Ekstraksi Ragi tempe
3
12.3533
Ektraksi Air
3
13.5333
Duncana
Ekstraksi Papain
3
13.8233
Sig.
1.000
.226
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Universitas Sumatera Utara
Lampiran 3. Analisis Sidik Ragam Kadar Air PLA dari berbagai metode
ekstraksi
Descriptives
Kadar air PLA talas kimpul
N Mean
Ekstraksi dengan air
Ekstraksi dengan
papain
Ekstraksi dengan ragi
tempe
Total
Std.
Deviation
Std.
Error
3
3.7100
95% Confidence Minim Maxim
Interval for Mean um
um
Lower Upper
Bound Bound
.01000 .00577 3.6852 3.7348
3.70
3.72
3
2.4800
.02000 .01155
2.4303 2.5297
2.46
2.50
3
2.8633
.14844 .08570
2.4946 3.2321
2.70
2.99
9
3.0178
.55020 .18340
2.5949 3.4407
2.46
3.72
ANOVA
Kadar Air PLA talas kimpul
Sum of Squares
Between Groups
2.377
Within Groups
.045
Total
2.422
df
2
6
8
Mean Square
1.188
.008
F
158.212
Sig.
.000
Kadar Air PLA Talas Kimpul
Kadar Air
N
Subset for alpha = 0.05
1
2
3
Ekstraksi dengan papain
3
2.4800
Ekstraksi dengan ragi tempe
3
2.8633
Duncana
Ekstraksi dengan air
3
3.7100
Sig.
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Universitas Sumatera Utara
Lampiran 4. Analisis sidik ragam kadar Serat PLA talas kimpul dari berbagai
metode ekstraksi
Descriptives
Persentase kadar serat PLA
Ekstraksi dengan air
Ekstraksi dengan
papain
Ekstraksi dengan ragi
tempe
Total
N
Mean
Std.
Deviation
Std.
Error
95% Confidence Mini Maxim
Interval for
mum
um
Mean
Lower Upper
Bound Bound
3
.4800
.01000
.00577
.4552
.5048
.47
.49
3
.4400
.01732
.01000
.3970
.4830
.42
.45
3
.3300
.01000
.00577
.3052
.3548
.32
.34
9
.4167
.06819
.02273
.3643
.4691
.32
.49
ANOVA
Persentase kadar serat PLA
Sum of
Squares
Between Groups
.036
Within Groups
.001
Total
.037
df
2
6
8
Mean
Square
.018
.000
F
108.600
Sig.
.000
Persentase kadar serat PLA
SeratKasar
N
Subset for alpha = 0.05
1
2
3
Ekstraksi dengan ragi tempe
3
.3300
Ekstraksi dengan papain
3
.4400
Duncana
Ekstraksi dengan air
3
.4800
Sig.
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Universitas Sumatera Utara
Lampiran 5. Analisis sidik ragam kadar pati PLA talas kimpul dari berbagai
metode ekstraksi
Oneway
Descriptives
Kadar Pati
N
Ekstraksi Air
Ekstraksi Papain
Ekstraksi Ragi Tempe
Total
Mean
3
3
3
9
Std.
Deviation
.1379
.1353
.1309
.1347
Std.
Error
.00145
.00040
.00199
.00331
.00084
.00023
.00115
.00110
95% Confidence Mini Maxi
Interval for
mum mum
Mean
Lower Upper
Bound Bound
.1343 .1415
.14
.14
.1343 .1363
.14
.14
.1259 .1358
.13
.13
.1321 .1372
.13
.14
ANOVA
Kadar Pati
Between Groups
Within Groups
Total
Sum of
Squares
.000
.000
.000
df
Mean Square
2
6
8
.000
.000
F
18.234
Sig.
.003
Kadar Pati
Perlakuan
N
Ekstraksi Ragi Tempe
Ekstraksi Papain
Duncana
Ekstraksi Air
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Subset for alpha = 0.05
1
2
3
.1309
3
.1353
3
.1379
1.000
.074
Universitas Sumatera Utara
Lampiran 6. Analisis sidik ragam kadar glukosa PLA talas kimpul dari
berbagai metode ekstraksi
Descriptives
Kadar glukosa
N
Ekstraksi air
Ekstraksi papain
Ekstraksi Ragi Tempe
Total
3
3
3
9
Mean
Std.
Deviation
.0529
.0537
.0541
.0536
Std.
Error
.00228
.00141
.00083
.00149
.00132
.00081
.00048
.00050
95% Confidence Mini Maxi
Interval for
mum mum
Mean
Lower Upper
Bound Bound
.0473 .0586
.05
.05
.0502 .0572
.05
.06
.0520 .0561
.05
.06
.0524 .0547
.05
.06
ANOVA
Kadar glukosa
Between Groups
Within Groups
Total
Sum of
Squares
.000
.000
.000
df
Mean Square
2
6
8
.000
.000
F
.382
Sig.
.698
Kadar Glukosa
Perlakuan
Ekstraksi air
Ekstraksi papain
Duncana
Ekstraksi Ragi Tempe
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
N
Subset for alpha
= 0.05
1
3
.0529
3
.0537
3
.0541
.439
Universitas Sumatera Utara
Lampiran 7. Analisis sidik ragam kadar gula pereduksi PLA talas kimpul dari
berbagai metode ekstraksi
Oneway
Descriptives
Gula reduksi
N
Ekstraksi Air
Ekstraksi Papain
Ekstraksi Ragi Tempe
Total
3
3
3
9
Mean
Std.
Deviation
.08047
.08243
.08377
.08222
Std.
Error
.000737
.001850
.001692
.001943
.000426
.001068
.000977
.000648
95% Confidence Mini Maxi
Interval for
mum mum
Mean
Lower Upper
Bound Bound
.07864 .08230 .080 .081
.07784 .08703 .081 .084
.07956 .08797 .082 .085
.08073 .08372 .080 .085
ANOVA
Gula reduksi
Between Groups
Within Groups
Total
Sum of
Squares
.000
.000
.000
df
Mean Square
2
6
8
.000
.000
F
3.632
Sig.
.093
Gula reduksi
Perlakuan
N
Ekstraksi Air
Ekstraksi Papain
Duncana
Ekstraksi Ragi Tempe
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Subset for alpha = 0.05
1
2
3
.08047
3
.08243
.08243
3
.08377
.162
.321
Universitas Sumatera Utara
Lampiran 8. Analisis sidik ragam total gula PLA talas kimpul dari berbagai
metode ekstraksi
Descriptives
Total gula
N
Ekstraksi air
Ekstraksi papain
Ekstraksi ragi tempe
Total
3
3
3
9
Mean
Std.
Deviation
1.7980
1.7846
1.7711
1.7846
Std.
Error
.28782
.06062
.08313
.15327
.16617
.03500
.04800
.05109
95% Confidence Mini Maxi
Interval for
mum mum
Mean
Lower Upper
Bound Bound
1.0830 2.5130
1.56
2.12
1.6340 1.9352
1.72
1.84
1.5646 1.9776
1.68
1.84
1.6668 1.9024
1.56
2.12
ANOVA
Total gula
Between Groups
Within Groups
Total
Sum of
Squares
.001
.187
.188
df
Mean Square
2
6
8
.001
.031
F
.017
Sig.
.983
Total gula
Perlakuan
Ekstraksi ragi tempe
Ekstraksi papain
Duncana
Ekstraksi air
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
N
Subset for alpha
= 0.05
1
3
1.7711
3
1.7846
3
1.7980
.862
Universitas Sumatera Utara
Lampiran 9. Analisis sidik ragam daya larut PLA talas kimpul dari berbagai
metode ekstraksi
Descriptives
Daya larut PLA
Ekstraksi dengan air
Ekstraksi dengan
papain
Ekstraksi dengan
ragi tempe
Total
N
Mean
Std.
Deviation
Std.
Error
3
76.8400
.54342 .31374
95% Confidence
Mini Maxi
Interval for Mean mum mum
Lower
Upper
Bound
Bound
75.4901 78.1899 76.36 77.43
3
80.6800
.17776 .10263
80.2384
81.1216 80.48 80.82
3
82.5200
.04359 .02517
82.4117
82.6283 82.47 82.55
9
80.0133
2.52614 .84205
78.0716
81.9551 76.36 82.55
ANOVA
Daya larut PLA
Between Groups
Within Groups
Total
Perlakuan
Sum of
Squares
50.394
.658
51.051
df
Mean Square
2
6
8
25.197
.110
F
229.898
Sig.
.000
Daya larut PLA
N
Subset for alpha = 0.05
1
2
3
Ekstraksi dengan air
3
76.8400
Ekstraksi dengan papain
3
80.6800
Duncana
Ekstraksi dengan ragi tempe
3
82.5200
Sig.
1.000
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Universitas Sumatera Utara
Lampiran 10. Analisis sidik ragam viskositas PLA talas kimpul dari berbagai
metode ekstraksi
Descriptives
Viskositas PLA
Ekstraksi dengan
air
Ekstraksi dengan
papain
Ekstraksi dengan
ragi tempe
Total
N
Mean
Std.
Std. Error
Deviation
95% Confidence Minim Maxi
Interval for Mean
um
mum
Lower
Upper
Bound
Bound
3
1.0267
.04163
.02404
.9232
1.1301
.98
1.06
3
.9700
.01000
.00577
.9452
.9948
.96
.98
3
.9667
.00577
.00333
.9523
.9810
.96
.97
9
.9878
.03632
.01211
.9599
1.0157
.96
1.06
ANOVA
Viskositas PLA
Between Groups
Within Groups
Total
Sum of
Squares
.007
.004
.011
df
Mean Square
2
6
8
.003
.001
F
5.482
Sig.
.044
Viskositas PLA
Viskositas (cP)
N
Ekstraksi dengan ragi tempe
3
Ekstraksi dengan papain
3
Duncana
Ekstraksi dengan air
3
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Subset for alpha = 0.05
1
2
.9667
.9700
1.0267
.875
1.000
Universitas Sumatera Utara
Lampiran 11. Analisis sidik ragam dextrose equivalen PLA talas kimpul dari
berbagai metode ekstraksi
Descriptives
Dextrose equivalen
N
Ekstraksi Air
Ekstraksi Papain
Ekstraksi Ragi Tempe
Total
3
3
3
9
Mean
Std.
Deviation
4.5483
4.6227
4.7374
4.6362
Std.
Error
.68818
.16025
.27979
.38884
95% Confidence Mini Maxi
Interval for
mum mum
Mean
Lower Upper
Bound Bound
.39732
.09252
.16154
.12961
2.8388
4.2247
4.0424
4.3373
6.2578
5.0208
5.4325
4.9350
3.79
4.48
4.46
3.79
5.12
4.80
5.02
5.12
ANOVA
Dextrose Equivalen
Between Groups
Within Groups
Total
Sum of
Squares
.054
1.155
1.210
df
Mean Square
2
6
8
Dextrose Equivalen
Perlakuan
Ekstraksi Air
Ekstraksi Papain
Duncana
Ekstraksi Ragi Tempe
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
F
.027
.193
Sig.
.141
N
3
3
3
.871
Subset for
alpha = 0.05
1
4.5483
4.6227
4.7374
.627
Universitas Sumatera Utara
Lampiran 12. Analisis sidik ragam derajat polimerisasi PLA talas kimpul dari
berbagai metode ekstraksi
Descriptives
Derajat polimerisasi
N
Ekstraksi air
Ekstraksi Papain
Ekstraksi Ragi
tempe
Total
Mean
3
3
Std.
Std. Error 95% Confidence Minim Maxi
Deviation
Interval for Mean
um
mum
Lower
Upper
Bound Bound
22.3495
3.60892 2.08361 13.3845 31.3146 19.52 26.41
21.6494
.74248
.42867 19.8050 23.4938 20.84 22.30
3
21.1578
1.25210
.72290 18.0474 24.2682
19.93
22.44
9
21.7189
2.01366
.67122 20.1711 23.2667
19.52
26.41
ANOVA
Derajat polimerisasi
Between Groups
Within Groups
Total
Sum of
Squares
2.152
30.287
32.439
df
Mean Square
2
6
8
1.076
5.048
F
Sig.
.213
.814
Derajat Polimerisasi
Perlakuan
Ekstraksi Ragi tempe
Ekstraksi Papain
Duncana
Ekstraksi air
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
N
3
3
3
Subset for
alpha = 0.05
1
21.1578
21.6494
22.3495
.552
Universitas Sumatera Utara
Lampiran 13. Hasil Analisa Sidik Ragam Kadar Glukosa Darah Tikus
Perminggu
Descriptives
Rerata Kadar Glukosa Darah perminggu
Normal
Negatif
Positif
Perlakuan
Total
N
Mean
5
5
5
5
20
68.5370
217.4220
156.3782
158.8000
150.2843
Std.
Deviation
Std.
Error
1.1070
.4951
1.0437
.4667
51.0784 22.8429
46.2869 20.7001
63.0430 14.0968
95% Confidence
Interval for Mean
Lower
Upper
Bound
Bound
67.1623
69.9116
216.1260 218.7180
92.9559 219.8005
101.3272 216.2728
120.7793 179.7893
Mini
mum
Maxi
mum
67.15
216.25
91.83
101.71
67.15
69.96
218.82
220.29
218.62
220.29
ANOVA
Kadar Glukosa Darah
Between Groups
Within Groups
Total
Sum of Squares
56498.756
19015.218
75513.973
df
3
16
19
Mean Square
18832.919
1188.451
F
15.847
Sig.
.000
Rerata Kadar Glukosa Darah Tiap Minggu
Perlakuan
N
Subset for alpha = 0.05
1
2
3
Normal
5
68.5370
Positif
5
156.3782
a
Duncan
Perlakuan
5
158.8000
Negatif
5
217.4220
Sig.
1.000
.913
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.
Universitas Sumatera Utara
Lampiran 14. Hasil Sidik Ragam Glucose Tolerance Test
Descriptives
Glucose Tolerance Test
menit ke 0
menit ke 30
menit ke 60
menit ke 90
menit ke 120
Total
N
Mean
3
3
3
3
3
15
112.5000
129.5333
101.9100
97.2067
75.0000
103.2300
Std.
Deviation
5.10565
5.62932
4.58429
2.35239
4.52019
18.98823
Std.
Error
95% Confidence
Interval for Mean
Lower
Upper
Bound
Bound
Mini
mum
Maxi
mum
2.94775 99.8169 125.1831 109.19
3.25009 115.5493 143.5173 126.10
2.64674 90.5220 113.2980 98.64
1.35815 91.3630 103.0503 94.64
2.60973 63.7712 86.2288 71.69
4.90274 92.7147 113.7453 71.69
118.38
136.03
107.15
99.26
80.15
136.03
ANOVA
Glucose Tolerance Test
Between Groups
Within Groups
Total
Sum of
Squares
4838.262
209.477
5047.739
df
4
10
14
Mean Square
1209.566
20.948
F
Sig.
57.742
Glucose Tolerance Test
Perlakuan
N
Subset for alpha = 0.01
1
2
3
menit ke 120
3
75.0000
menit ke 90
3
97.2067
menit ke 60
3
101.9100 101.9100
Duncana
menit ke 0
3
112.5000
menit ke 30
3
Sig.
1.000
.237
.018
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
.000
4
129.5333
1.000
Universitas Sumatera Utara
Lampiran 15. Hasil Sidik Ragam Berat Badan Tikus
Descriptives
Berat Badan Tikus
Normal
Negatif
Positif
Perlakuan
Total
N
Mean
6
6
6
6
24
220.666
176.333
200.000
200.833
199.458
Std.
Deviation
1.0328
6.3770
6.4187
6.6156
16.8806
Std.
Error
.4216
2.6034
2.6204
2.7008
3.4457
95% Confidence Interval
for Mean
Lower
Upper
Bound
Bound
219.5828
221.7505
169.6410
183.0256
193.2640
206.7360
193.8906
207.7760
192.3303
206.5864
Mini
mum
Maxi
mum
219.00
169.00
188.00
191.00
169.00
222.00
187.00
206.00
208.00
222.00
ANOVA
Berat Badan Tikus
Between Groups
Within Groups
Total
Sum of Squares
5920.458
633.500
6553.958
df
3
20
23
Mean Square
1973.486
31.675
F
62.304
Sig.
.000
Berat Badan Tikus
Perlakuan
N
Subset for alpha = 0.01
1
2
3
Negatif
6
176.3333
Positif
6
200.0000
a
Duncan
Perlakuan
6
200.8333
Normal
6
220.6667
Sig.
1.000
.800
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6.000.
Universitas Sumatera Utara
Lampiran 16. Dokumentasi Kegiatan Penelitian
Penimbangan berat badan tikus
Nicotinamide dan Streptozotocin
Pengambilan darah secara retro orbital plexus
PLA setelah dilarutkan dengan aquades
Proses analisa in situ pada shaker water bath (pada suhu 37 0 C)
Universitas Sumatera Utara
Injeksi Nicotinamide dan streptozotocin
Kandang Percobaan
Hasil sentrifugasi digesta
Darah tikus setelah oksidasi
Penampakan secara visual tikus
P1, P2, P3, P4
Universitas Sumatera Utara
Lampiran 17. Kromatogram Gas Chromatography Analisa SCFA
Kromatogram Standar SCFA (Short Chain Fatty Acid)
Kromatogram tikus kontrol normal 1
Universitas Sumatera Utara
Kromatogram tikus kontrol normal 2
Kromatogram tikus kontrol normal 2
Universitas Sumatera Utara
Kromatogram tikus kelompok negatif 1
Kromatogram tikus kelompok negatif 2
Universitas Sumatera Utara
Kromatogram tikus kelompok negatif 3
Kromatogram tikus kelompok positif 1
Universitas Sumatera Utara
Kromatogram tikus kelompok positif 2
Kromatogram tikus kelompok positif 3
Universitas Sumatera Utara
Kromatogram tikus kelompok perlakuan 1
Kromatogram tikus kelompok perlakuan 2
Universitas Sumatera Utara
Kromatogram tikus kelompok perlakuan 3
Universitas Sumatera Utara