Efek Antihiperurisemia Ekstrak Etanol Biji Alpukat (Persea americana Mill.) pada Mencit Putih Jantan

Tanaman Alpukat

Lampiran 3. (Lanjutan)

Simplisia biji alpukat

Lampiran 4. Hasil pemeriksaan mikroskopik serbuk simplisia biji alpukat

Keteranga: 1 = parenkim 2 = xylem 3 = epidermis 4 = amilum

1 2 3 4

1. Perhitungan Penetapan Kadar Air

a. Berat sampel = 5,006 g Volume air = 0,15 ml Kadar air = ,

, � % = , %

b. Berat sampel = 5,015 Volume air = 0,30 ml Kadar air = ,

, x % = , %

c. Berat sampel = 5,010 Volume air = 0,20 ml Kadar air = ,

, x % = 3,992%

Kadar air rata-rata = , + , + , %= , %

No Berat sampel (g) Volume awal (ml) Volume akhir (ml)

1 5,006 1,0 1,15

2 5,015 1,15 1,45

3 5,010 1,45 1,65

Kadar air simplisia = e i

Lampiran 5. (Lanjutan)

2. Perhitungan Penetapan Kadar Sari Larut dalam Air

a. Berat sampel = 5,014 g Berat sari = 0,294 g Kadar sari = ,

, x x % = , % b. Berat sampel = 5,010 g

Berat sari = 0,201 g Kadar sari = ,

, x x % = , % c. Berat sampel = 5,008 g

Berat sari = 0,261 Kadar sari = ,

, x x % = , %

Kadar sari rata-rata = , + , + , %= , %

No Berat sampel (g) Berat cawan kosong (g) Berat cawan sari (g)

1 5,014 42,807 43,101

2 5,010 45,780 45,981

3 5,008 47,840 48,101

Kadar sari = e i

3. Perhitungan Penetapan Kadar Sari Larut dalam Etanol

a. Berat sampel = 5,010 g Berat sari = 0,227 g Kadar sari = ,

, x x % = , % b. Berat sampel = 5,003 g

Berat sari = 0,198 g Kadar sari = ,

, x x % = , % c. Berat sampel = 5,008 g

Berat sari = 0,178 g Kadar sari = ,

, x x % = , % Kadar sari rata-rata = , + , + , %= , %

No Berat sampel (g) Berat cawan kosong (g) Berat cawan sari (g)

1 5,004 42,958 43,185

2 5,003 45,008 45,206

3 5,008 46,834 47,012

Kadar sari = e i

Lampiran 5. (Lanjutan)

4. Perhitungan Penetapan Kadar Abu Total

a. Berat sampel = 2,005 g Berat abu = 0,154 g Kadar abu = ,

, x % = , %

b. Berat sampel = 2,011 g Berat abu = 0,161 g Kadar abu = ,

, x % = , %

c. Berat sampel = 2,008 g Berat abu = 0,144 g Kadar abu = ,

, x % = , %

Kadar abu total rata-rata = , + , + , %= , % Kadar abu total = e

5. Perhitungan Penetapan Kadar Abu Tidak Larut Asam

a. Berat sampel = 2,005 g Berat abu = 0,016 g Kadar abu = ,

, x % = , %

b. Berat sampel = 2,011 g Berat abu = 0,018 g Kadar abu = ,

, x % = , %

c. Berat sampel = 2,008 g Berat abu = 0,018 g Kadar abu = ,

, x % = , %

Kadar abu tak larut asam rata-rata = , + , + , %= , %

Kadar abu tidak larut asam = e

Lampiran 6. Perhitungan Hasil Pemeriksaan Karakterisasi Ekstrak 1. Perhitungan Penetapan Kadar Air

a. Berat sampel = 5,007 g Volume air = 0,1 ml Kadar air = ,

, � % = , %

b. Berat sampel = 5,013 Volume air = 0,1 ml Kadar air = ,

, x % = , %

c. Berat sampel = 5,011 Volume air = 0,1 ml Kadar air = ,

, x % = 1,996%

Kadar air rata-rata = , + , + , %= , %

No Berat sampel (g) Volume awal (ml) Volume akhir (ml)

1 5,007 1,0 1,10

2 5,013 1,10 1,20

3 5,011 1,20 1,30

Kadar air simplisia = e i

2. Perhitungan Penetapan Kadar Sari Larut dalam Air

a. Berat sampel = 5,012 g Berat sari = 0,023 g Kadar sari = ,

, x x % = , %

b. Berat sampel = 5,015 g Berat sari = 0,020 g Kadar sari = ,

, x x % = , %

c. Berat sampel = 5,008 g Berat sari = 0,039 Kadar sari = ,

, x x % = , %

Kadar sari rata-rata = , + , + , %= , %

No Berat sampel (g) Berat cawan kosong (g) Berat cawan sari (g)

1 5,012 43,105 43,128

2 5,015 45,081 45,101

3 5,008 42,134 42,173

Kadar sari = e i

Lampiran 6. (Lanjutan)

3. Perhitungan Penetapan Kadar Sari Larut dalam Etanol

a. Berat sampel = 5,015 g Berat sari = 0,231 g Kadar sari = ,

, x x % = , % b. Berat sampel = 5,008 g

Berat sari = 0,285 g Kadar sari = ,

, x x % = , % c. Berat sampel = 5,010 g

Berat sari = 0,199 g Kadar sari = ,

, x x % = , % Kadar sari rata-rata = , + , + , %= , %

No Berat sampel (g) Berat cawan kosong (g) Berat cawan sari (g)

1 5,015 42,113 42,344

2 5,008 47,158 47,443

3 5,010 45,916 46,115

Kadar sari = e i

4. Perhitungan Penetapan Kadar Abu Total

a. Berat sampel = 2,015 g Berat abu = 0,119 g Kadar abu = ,

, x % = , %

b. Berat sampel = 2,008 g Berat abu = 0,109 g Kadar abu = ,

, x % = , %

c. Berat sampel = 2,012 g Berat abu = 0,111 g Kadar abu = ,

, x % = , %

Kadar abu total rata-rata = , + , + , %= , % Kadar abu total = e

Lampiran 6. (Lanjutan)

5. Perhitungan Penetapan Kadar Abu Tidak Larut Asam

d. Berat sampel = 2,008 g Berat abu = 0,015 g Kadar abu = ,

, x % = , %

e. Berat sampel = 2,016 g Berat abu = 0,016 g Kadar abu = ,

, x % = , %

f. Berat sampel = 2,006 g Berat abu = 0,014 g Kadar abu = ,

, x % = , %

Kadar abu tak larut asam rata-rata = , + , + , %= , % Kadar abu tidak larut asam = e

Serbuk simplisia Biji alpukat

Simplisia

Dicuci, ditiriskan, dipotong menjadi bagian kecil dan ditimbang sebagai berat basah

Dikeringkan dalam lemari pengering

Ditimbang berat kering Dihaluskan

Karakterisasi simplisia Skrining Fitokimia Ekstraksi

Perkolat

Ekstrak kental

Hasil

Diperkolasi dengan

etanol 96%

Diuapkan dengan

rotary evaporator

Diuji pada

mencit hiperurisemia

Makroskopik

Mikroskopik

Penetapan kadar air

Penetapan kadar sari

yang larut dalam air

Penetapan kadar sari

yang larut dalam etanol

Penetapan kadar abu

total

Penetapan kadar abu

yang tidak larut dalam asam Pemeriksaan: -Alkaloid -Flavonoid -Glikosida -Saponin -Tanin -Steroid/triterpenoid

Lampiran 8. Bagan Pembuatan Ekstrak

Dimasukkan ke dalam sebuah bejana Dituangi dengan pelarut etanol 96% dengan perbandingan 1 : 7,5

Ditutup

Dibiarkan selama 24 jam terlindung dari cahaya sambil sesekali di aduk

Diserkai, diperas

Dituang ke dalam perkolator

Direndam selama 24 jam terlindung dari cahaya

Dibiarkan menetes sebanyak 1 tetes per 3 detik

Ditampung tetesan ke dalam wadah terlindung dari cahaya hingga tetesan berwarna hampir bening

Dipekatkan dengan alat rotary evaporator pada temperature ±40oC

Dipekatkan lagi dengan alat blow dryer 300 g serbuk simplisia

Ampas Maserat

Ekstrak cair

Ekstrak kental 55,43 g

Rotary Evaporator

Lampiran 9. (lanjutan)

Seperangkat alat pengukur kadar asam urat EasyTouch® (Vial strip asam urat, GCU Meter, strip asam urat)

Kristal potassium oxonate Larutan potassium oxonate

Lampiran 9. (lanjutan)

a. Larutan potassium oxonate 0,8%, dosis 300 mg/kg bb (bb mencit, mis : 25 g) Sebanyak 80 mg kalium oksonat dilarutkan dalam 10 ml akua bidestilata steril. Maka kadar larutan kalium oksonat = � = 0,8%

Jumlah obat = dosis x bb = � � �

� = 7,5 mg Konsentrasi obat 0,8% = , � = � = 8 mg/ml Jumlah larutan obat = ℎ

� =

, �

�/ = 0,9375 ml

b. Suspensi allopurinol 0,4%, dosis 10 mg/kg bb (bb mencit, mis : 25 g) Tiap tablet allopurinol mengandung 100 mg allopurinol dilarutkan dalam 25 ml CMC-Na 0,5%. Maka kadar suspensi allopurinol = � = 0,4%

Jumlah obat = dosis x bb = � � �

� = 0,25 mg Konsentrasi obat 0,4% = , � = � = 4 mg/ml Jumlah larutan obat = ℎ

� =

, �

�/ = 0,0625 ml

c. Suspensi EEBA 1%, dosis 50 mg/kg bb, 100 mg/kg bb, 200 mg/kg bb (bb mencit, mis : 25 g)

Sebanyak 250 mg EEBA disuspensikan dalam 25 ml CMC-Na 0,5%. Maka kadar suspensi EEBA = � = 1%

Jumlah obat = dosis x bb = � � �

� = 1,25 mg Konsentrasi obat 0,8% = � = � = 10 mg/ml Jumlah larutan obat = ℎ

� =

, �

Lampiran 11. Hasil pengukuran kadar asam urat sesudah pemberian EEBA dan allopurinol Perlakuan Berat Badan (g) KAU Normal 1 jam setelah diinduksi

Kadar Asam Urat (mg/dl) 30' 60' 90' 120'

Suspensi CMC-Na dosis 1% BB

20 1.7 10.4 10.8 11.1 11.5 12.1

23.5 1.5 2.6 2.7 2.8 3 3

20 1.5 2.3 2.4 2.6 2.6 2.6

23.3 1.5 2.7 2.7 2.7 2.6 2.7

27.2 1.6 3 3.3 3.4 3.4 3.4

Suspensi Allopurinol dosis 10 mg/kg BB

25.2 1.5 3.3 3 2.7 2.3 2

25.2 1.6 4.5 3.8 2.7 2.5 2.1

21.1 1.7 7.4 6.8 5.8 4.2 3.9

25.7 1.5 6 5.3 4.5 4 3.1

26.5 1.7 8.1 7.6 6.5 5.4 4.3

Suspensi EEBA dosis 50 mg/kg BB

25.2 1.5 2.7 2.6 2.4 2 1.9

23.8 1.7 5.4 5 4.6 3.9 3

23.2 1.5 2.6 2.5 2.3 2 1.9

25.2 1.5 2.7 2.6 2.4 2 1.9

25.2 1.5 2.9 2.7 2.4 2.2 2

Suspensi EEBA dosis 100 mg/kg

BB

26.9 1.6 4.6 4 3.5 2.8 2

26.9 1.6 3.8 3.5 2.8 2.4 2.1

23 1.5 2.7 2.5 2.3 2.1 1.8

26.2 1.7 5.1 4.9 4 3.5 2.8

27.8 1.5 2.8 2.6 2.4 2.2 1.9

Suspensi EEBA dosis 200 mg/kg

BB

24.3 1.5 2.8 2.6 2.3 2 1.8

24.3 1.6 4.4 3.8 3.4 2.5 2

25 1.7 5.3 4.8 4.2 3.5 2.5

20.6 1.6 3.6 2.4 2.2 2 1.9

pemberian EEBA dan allopurinol

Perlakuan

Berat Badan (g)

% Penurunan Kadar Asam Urat

30' 60' 90' 120'

Suspensi CMC-Na dosis 1% BB

20 -3.85 -6.73 -10.58 -16.35 23.5 -3.85 -7.69 -15.38 -15.38 20 -4.35 -13.04 -13.04 -13.04

23.3 0.00 0.00 3.70 0.00

27.2 -10.00 -13.33 -13.33 -13.33

Rata-Rata -4.41 -8.16 -9.73 -11.62

Suspensi Allopurinol dosis 10 mg/kg BB

25.2 9.09 18.18 30.30 39.39 25.2 15.56 40.00 44.44 53.33 21.1 8.11 21.62 43.24 47.30 25.7 11.67 25.00 33.33 48.33 26.5 6.17 19.75 33.33 46.91

Rata-Rata 10.12 24.91 36.93 47.05

Suspensi EEBA dosis 50 mg/kg BB

25.2 16.67 27.78 33.33 44.44 23.8 7.41 14.81 27.78 44.44 23.2 3.85 11.54 23.08 26.92 25.2 3.70 11.11 25.93 29.63 25.2 8.00 17.24 24.14 31.03

Rata-Rata 7.93 16.50 26.85 35.29

Suspensi EEBA dosis 100 mg/kg BB

26.9 13.04 23.90 39.13 56.52 26.9 7.89 26.32 36.84 47.37 23 7.41 14.81 22.22 33.33 26.2 3.92 21.57 31.37 45.10 27.8 7.14 14.29 25.00 35.71

Rata-Rata 7.88 20.18 30.91 43.61

Suspensi EEBA dosis 200 mg/kg BB

24.3 7.14 17.86 28.57 35.71 24.3 13.64 22.73 43.18 54.55 25 9.43 20.75 33.96 52.83 20.6 16.67 27.78 36.11 47.22 24.5 13.33 23.33 30.00 40.00

Lampiran 13. Hasil Analisis One way Anava rata-rata persen penurunan kadar asam urat pada menit ke-30

Post-Hoc Tests Multiple Comparison Tuckey HSD

(I) Perlakuan (J) Perlakuan

Mean Difference

(I-J)

Std.

Error Sig.

95% Confidence Interval Lower Bound Upper Bound kontrol_CMC allopurinol -14.53000 2.50913 .000 -22.0383 -7.0217

EEBA_50 -12.33600 2.50913 .001 -19.8443 -4.8277 EEBA_100 -12.29000 2.50913 .001 -19.7983 -4.7817 EEBA_200 -16.45200 2.50913 .000 -23.9603 -8.9437 allopurinol kontrol_CMC 14.53000 2.50913 .000 7.0217 22.0383 EEBA_50 2.19400 2.50913 .903 -5.3143 9.7023 EEBA_100 2.24000 2.50913 .896 -5.2683 9.7483 EEBA_200 -1.92200 2.50913 .937 -9.4303 5.5863 EEBA_50 kontrol_CMC 12.33600 2.50913 .001 4.8277 19.8443 allopurinol -2.19400 2.50913 .903 -9.7023 5.3143 EEBA_100 .04600 2.50913 1.000 -7.4623 7.5543 EEBA_200 -4.11600 2.50913 .490 -11.6243 3.3923 EEBA_100 kontrol_CMC 12.29000 2.50913 .001 4.7817 19.7983 allopurinol -2.24000 2.50913 .896 -9.7483 5.2683 EEBA_50 -.04600 2.50913 1.000 -7.5543 7.4623 EEBA_200 -4.16200 2.50913 .480 -11.6703 3.3463 EEBA_200 kontrol_CMC 16.45200 2.50913 .000 8.9437 23.9603 allopurinol 1.92200 2.50913 .937 -5.5863 9.4303

Homogeneous Subsets

persenpenurunanKAU Perlakuan

N

Subset for alpha = 0.05

1 2

Tukey HSDa

kontrol_CMC 5 -4.4100 EEBA_100 5 7.8800

EEBA_50 5 7.9260

allopurinol 5 10.1200 EEBA_200 5 12.0420

Sig. 1.000 .480

Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 5.000

Lampiran 14. Hasil Analisis One way Anava rata-rata persen penurunan kadar asam urat pada menit ke-60

Post-Hoc Tests Multiple Comparison Tuckey HSD (I) Perlakuan (J) Perlakuan Mean Differenc e (I-J) Std.

Error Sig.

95% Confidence Interval Lower Bound Upper Bound kontrol_CMC allopurinol

-33.06800

3.96071 .000 -44.9199

-21.2161

EEBA_50

-24.65400

3.96071 .000 -36.5059

-12.8021

EEBA_100

-28.33600

3.96071 .000 -40.1879

-16.4841

EEBA_200

-30.64800

3.96071 .000 -42.4999

-18.7961 allopurinol kontrol_CMC 33.06800 3.96071 .000 21.2161 44.9199 EEBA_50 8.41400 3.96071 .249 -3.4379 20.2659 EEBA_100 4.73200 3.96071 .754 -7.1199 16.5839 EEBA_200 2.42000 3.96071 .972 -9.4319 14.2719 EEBA_50 kontrol_CMC 24.65400 3.96071 .000 12.8021 36.5059

allopurinol -8.41400 3.96071 .249 -20.2659

3.4379 EEBA_100 -3.68200 3.96071 .882

-15.5339

8.1699 EEBA_200 -5.99400 3.96071 .566

-17.8459

5.8579 EEBA_100 kontrol_CMC 28.33600 3.96071 .000 16.4841 40.1879

allopurinol -4.73200 3.96071 .754 -16.5839

7.1199 EEBA_50 3.68200 3.96071 .882 -8.1699 15.5339 EEBA_200 -2.31200 3.96071 .976

-14.1639

9.5399 EEBA_200

kontrol_CMC 30.64800 3.96071 .000 18.7961 42.4999 allopurinol -2.42000 3.96071 .972

-14.2719

9.4319 EEBA_50 5.99400 3.96071 .566 -5.8579 17.8459 EEBA_100 2.31200 3.96071 .976 -9.5399 14.1639 *. The mean difference is significant at the 0,05 level

Homogeneous Subsets

persenpenurunanKAU

Perlakuan

N

Subset for alpha = 0.05

1 2

Tukey HSDa kontrol_CMC 5 -8.1580

EEBA_50 5 16.4960

EEBA_100 5 20.1780

EEBA_200 5 22.4900

allopurinol 5 24.9100

Sig. 1.000 .249

Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 5.000.

Lampiran 15. Hasil Analisis One way Anava rata-rata persen penurunan kadar asam urat pada menit ke-90

Post-Hoc Tests Multiple Comparison Tuckey HSD

(I) Perlakuan (J) Perlakuan

Mean Difference

(I-J)

Std.

Error Sig.

95% Confidence Interval Lower Bound Upper Bound kontrol_CMC allopurinol -46.65400 4.03786 .000 -58.7368 -34.5712

EEBA_50 -36.57800 4.03786 .000 -48.6608 -24.4952 EEBA_100 -40.63800 4.03786 .000 -52.7208 -28.5552 EEBA_200 -44.09000 4.03786 .000 -56.1728 -32.0072 allopurinol kontrol_CMC 46.65400 4.03786 .000 34.5712 58.7368 EEBA_50 10.07600 4.03786 .131 -2.0068 22.1588 EEBA_100 6.01600 4.03786 .580 -6.0668 18.0988 EEBA_200 2.56400 4.03786 .967 -9.5188 14.6468 EEBA_50 kontrol_CMC 36.57800 4.03786 .000 24.4952 48.6608 allopurinol -10.07600 4.03786 .131 -22.1588 2.0068 EEBA_100 -4.06000 4.03786 .850 -16.1428 8.0228 EEBA_200 -7.51200 4.03786 .369 -19.5948 4.5708 EEBA_100 kontrol_CMC 40.63800 4.03786 .000 28.5552 52.7208 allopurinol -6.01600 4.03786 .580 -18.0988 6.0668 EEBA_50 4.06000 4.03786 .850 -8.0228 16.1428 EEBA_200 -3.45200 4.03786 .910 -15.5348 8.6308 EEBA_200 kontrol_CMC 44.09000 4.03786 .000 32.0072 56.1728 allopurinol -2.56400 4.03786 .967 -14.6468 9.5188 EEBA_50 7.51200 4.03786 .369 -4.5708 19.5948 EEBA_100 3.45200 4.03786 .910 -8.6308 15.5348

Homogeneous Subsets

persenpenurunanKAU

Perlakuan

N

Subset for alpha = 0.05

1 2

Tukey HSDa kontrol_CMC 5 -9.7260

EEBA_50 5 26.8520

EEBA_100 5 30.9120

EEBA_200 5 34.3640

allopurinol 5 36.9280

Sig. 1.000 .131

Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 5.000.

Lampiran 16. Hasil Analisis One way Anava rata-rata persen penurunan kadar asam urat pada menit ke-120

Post-Hoc Tests Multiple Comparison Tuckey HSD (I) Perlakuan (J) Perlakuan Mean Difference (I-J) Std.

Error Sig.

95% Confidence Interval Lower Bound Upper Bound kontrol_CMC

allopurinol -58.67200 4.85430 .000 -73.1979

-44.1461 EEBA_50 -46.91200 4.85430 .000

-61.4379

-32.3861 EEBA_100 -55.22600 4.85430 .000

-69.7519

-40.7001 EEBA_200 -57.68200 4.85430 .000

-72.2079

-43.1561 allopurinol

kontrol_CMC 58.67200 4.85430 .000 44.1461 73.1979 EEBA_50 11.76000 4.85430 .150 -2.7659 26.2859 EEBA_100 3.44600 4.85430 .952

-11.0799

17.9719 EEBA_200 .99000 4.85430 1.000

-13.5359

15.5159 EEBA_50

kontrol_CMC 46.91200 4.85430 .000 32.3861 61.4379 allopurinol -11.76000 4.85430 .150

-26.2859

2.7659 EEBA_100 -8.31400 4.85430 .449

-22.8399

6.2119 EEBA_200 -10.77000 4.85430 .213

-25.2959

3.7559 EEBA_100

kontrol_CMC 55.22600 4.85430 .000 40.7001 69.7519 allopurinol -3.44600 4.85430 .952

-17.9719

11.0799 EEBA_50 8.31400 4.85430 .449 -6.2119 22.8399 EEBA_200 -2.45600 4.85430 .986

-16.9819

12.0699 EEBA_200

Homogeneous Subsets

Perlakuan

N

Subset for alpha = 0.05

1 2

Tukey HSDa

kontrol_CMC 5 -11.6200 EEBA_50 5 35.2920 EEBA_100 5 43.6060 EEBA_200 5 46.0620 allopurinol 5 47.0520

Sig. 1.000 .150

Means for groups in homogeneous subsets are displayed. b. Uses Harmonic Mean Sample Size = 5.000

Lampiran 17. Hasil Analisis One way Anava rata-rata persen penurunan kadar asam urat pada menit ke-120

ANOVA

Sum of Squares df Mean Square F Sig. Between Groups 318.579 2 159.290 2.119 .163 Within Groups 901.904 12 75.159

Total 1220.483 14

Homogenous Subset perlakuan

N

Subset for alpha = 0.05

1 Tukey HSDa EEBA 50 5 35.2920

EEBA100 5 43.6060 EEBA 200 5 46.0620

Sig. .164

Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 5.000.

Multiple Comparisons (I) perlakuan (J) perlakuan

Mean Difference

(I-J)

Std.

Error Sig.

95% Confidence Interval Lower Bound Upper Bound Tukey HSD

EEBA 50 EEBA100 -8.31400 5.48302 .318 -22.9419 6.3139 EEBA 200 -10.77000 5.48302 .164 -25.3979 3.8579 EEBA100 EEBA 50 8.31400 5.48302 .318 -6.3139 22.9419 EEBA 200 -2.45600 5.48302 .896 -17.0839 12.1719 EEBA 200 EEBA 50 10.77000 5.48302 .164 -3.8579 25.3979

Homogeneous Subsets

persenpenurunanKAU

Perlakuan

N

Subset for alpha = 0.05

1 2

Tukey HSDa kontrol_CMC 5 -8.1580

EEBA_50 5 16.4960

EEBA_100 5 20.1780 EEBA_200 5 22.4900 allopurinol 5 24.9100

Sig. 1.000 .249

Means for groups in homogeneous subsets are displayed.

a. Uses Harmonic Mean Sample Size = 5.000.

80

Perlakuan

Mean Difference

(I-J)

Std.

Error Sig.

Interval Lower Bound

Upper Bound kontrol_CMC allopurinol -46.65400 4.03786 .000 -58.7368 -34.5712

EEBA_50 -36.57800 4.03786 .000 -48.6608 -24.4952 EEBA_100 -40.63800 4.03786 .000 -52.7208 -28.5552 EEBA_200 -44.09000 4.03786 .000 -56.1728 -32.0072 allopurinol kontrol_CMC 46.65400 4.03786 .000 34.5712 58.7368 EEBA_50 10.07600 4.03786 .131 -2.0068 22.1588 EEBA_100 6.01600 4.03786 .580 -6.0668 18.0988 EEBA_200 2.56400 4.03786 .967 -9.5188 14.6468 EEBA_50 kontrol_CMC 36.57800 4.03786 .000 24.4952 48.6608 allopurinol -10.07600 4.03786 .131 -22.1588 2.0068 EEBA_100 -4.06000 4.03786 .850 -16.1428 8.0228 EEBA_200 -7.51200 4.03786 .369 -19.5948 4.5708 EEBA_100 kontrol_CMC 40.63800 4.03786 .000 28.5552 52.7208 allopurinol -6.01600 4.03786 .580 -18.0988 6.0668 EEBA_50 4.06000 4.03786 .850 -8.0228 16.1428 EEBA_200 -3.45200 4.03786 .910 -15.5348 8.6308 EEBA_200 kontrol_CMC 44.09000 4.03786 .000 32.0072 56.1728 allopurinol -2.56400 4.03786 .967 -14.6468 9.5188 EEBA_50 7.51200 4.03786 .369 -4.5708 19.5948 EEBA_100 3.45200 4.03786 .910 -8.6308 15.5348

Homogeneous Subsets

persenpenurunanKAU

Perlakuan

N

Subset for alpha = 0.05

1 2

Tukey HSDa kontrol_CMC 5 -9.7260

EEBA_50 5 26.8520

EEBA_100 5 30.9120 EEBA_200 5 34.3640 allopurinol 5 36.9280

Sig. 1.000 .131

Means for groups in homogeneous subsets are displayed.

a. Uses Harmonic Mean Sample Size = 5.000.

82

(I) Perlakuan (J) Perlakuan Mean Difference (I-J) Std.

Error Sig.

95% Confidence Interval Lower Bound Upper Bound kontrol_CMC

allopurinol -58.67200 4.85430 .000 -73.1979

-44.1461 EEBA_50 -46.91200 4.85430 .000

-61.4379

-32.3861 EEBA_100 -55.22600 4.85430 .000

-69.7519

-40.7001 EEBA_200 -57.68200 4.85430 .000

-72.2079

-43.1561 allopurinol

kontrol_CMC 58.67200 4.85430 .000 44.1461 73.1979 EEBA_50 11.76000 4.85430 .150 -2.7659 26.2859 EEBA_100 3.44600 4.85430 .952

-11.0799

17.9719 EEBA_200 .99000 4.85430 1.000

-13.5359

15.5159 EEBA_50

kontrol_CMC 46.91200 4.85430 .000 32.3861 61.4379 allopurinol -11.76000 4.85430 .150

-26.2859

2.7659 EEBA_100 -8.31400 4.85430 .449

-22.8399

6.2119 EEBA_200 -10.77000 4.85430 .213

-25.2959

3.7559 EEBA_100

kontrol_CMC 55.22600 4.85430 .000 40.7001 69.7519 allopurinol -3.44600 4.85430 .952

-17.9719

11.0799 EEBA_50 8.31400 4.85430 .449 -6.2119 22.8399 EEBA_200 -2.45600 4.85430 .986

-16.9819

12.0699 EEBA_200

kontrol_CMC 57.68200 4.85430 .000 43.1561 72.2079 allopurinol -.99000 4.85430 1.000

-15.5159

13.5359 EEBA_50 10.77000 4.85430 .213 -3.7559 25.2959 EEBA_100 2.45600 4.85430 .986

-12.0699

16.9819

*. The mean difference is significant at the 0,05 level

Homogeneous Subsets

Perlakuan

N

Subset for alpha = 0.05 1 2 Tukey

HSDa

kontrol_CMC 5 -11.6200

EEBA_50 5

35.2920 EEBA_100 5

43.6060 EEBA_200 5

46.0620 allopurinol 5

47.0520 Sig.

1.000 .150

Means for groups in homogeneous subsets are displayed.

b.

Uses Harmonic Mean Sample Size = 5.000

84

Within Groups 901.904 12 75.159 Total 1220.483 14

Homogenous Subset

perlakuan

N

Subset for alpha = 0.05

1 Tukey HSDa EEBA 50 5 35.2920

EEBA100 5 43.6060 EEBA 200 5 46.0620

Sig. .164

Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 5.000.

Multiple Comparisons

(I) perlakuan (J) perlakuan

Mean Difference

(I-J)

Std.

Error Sig.

95% Confidence Interval Lower Bound

Upper Bound Tukey

HSD

EEBA 50 EEBA100 -8.31400 5.48302 .318 -22.9419 6.3139 EEBA 200 -10.77000 5.48302 .164 -25.3979 3.8579 EEBA100 EEBA 50 8.31400 5.48302 .318 -6.3139 22.9419 EEBA 200 -2.45600 5.48302 .896 -17.0839 12.1719 EEBA 200 EEBA 50 10.77000 5.48302 .164 -3.8579 25.3979 EEBA100 2.45600 5.48302 .896 -12.1719 17.0839