Aktivitas Nefroprotektif Ekstrak Etanol Temu Mangga (Curcuma mangga Val) Pada Mencit Jantan yang Diinduksi Parasetamol
AKTIVITAS NEFROPROTEKTIF EKSTRAK ETANOL TEMU MANGGA
(Curcuma mangga Val) PADA MENCIT JANTAN YANG DIINDUKSI
PARASETAMOL
ABSTRAK
Temu mangga mengandung antioksidan yang dapat menangkal radikal
bebas dan mengurangi terbentuknya N-acetyl-parabenzoquinoneimine (NAPQI)
yang dihasilkan dari metabolisme parasetamol. Penelitian ini bertujuan untuk
mengidentifikasi apakah ekstrak etanol temu mangga memiliki aktivitas
nefroprotektif yaitu dapat menurunkan kadar serum kreatinin dan melindungi sel
ginjal dari nekrosis yang disebabkan oleh parasetamol terhadap mencit jantan.
Aktivitas ekstrak dievaluasi menggunakan kelompok kontrol normal,
kelompok kontrol negatif, kelompok kontrol positif dan kelompok perlakuan
ekstrak etanol temu mangga (EETM) dengan dosis 100 mg/kg bb, 200 mg/kg bb,
dan 400 mg/kg bb. Mencit kelompok kontrol normal dan kontrol negatif diberikan
CMC Na 0,5% selama 7 hari. Mencit kelompok kontrol positif diberikan
Curcuma® 58 mg/kg bb selama 7 hari. Ekstrak akan diberikan secara oral pada
mencit dengan dosis 100 mg/kg bb, 200 mg/kg bb dan 400 mg/kg bb. EETM
diberikan selama 7 hari. Untuk kelompok kontrol negatif, kontrol positif,
kelompok perlakuan EETM 100 mg/kg bb, EETM 200 mg/kg bb, EETM 400
mg/kg bb, pada hari ke-7, setelah 1 jam perlakuan, mencit diinduksi dengan
parasetamol 1,05 g/kg bb. Serum kadar kreatinin darah semua kelompok diuji.
Semua ginjal dipindahkan ke larutan formalin 10% untuk diuji histopatologi. Data
dianalisis dengan menggunakan uji One-Way ANOVA dan dilanjutkan dengan uji
Post Hoc Tuckey.
Berdasarkan hasil pengujian, pemberian parasetamol menyebabkan
kerusakan ginjal ditandai dengan peningkatan serum kreatinin serta gambaran
kerusakan histopatologi. Berdasarkan hasil analisis statistik, pemberian EETM
dosis 100, 200, dan 400 mg/kg bb mampu menghambat peningkatan kadar
kreatinin dan menunjukkan perbandingan berbeda signifikan dengan kontrol
negatif (p = 0,000) dan tidak berbeda signifikan dibandingkan kontrol positif (p =
0,432). Pada gambaran histopatologi sel ginjal dosis 400 mg/kg bb tidak
menunjukkan adanya kerusakan sel.
EETM dosis 400 mg/kg bb mempunyai aktivitas nefroprotektif dengan
kadar serum kreatinin = 0,5320 ± 0,0804 mg/dl, dan pada dosis 100 mg/kg bb,
200 mg/kg bb, 400 mg/kg bb tidak menunjukkan kerusakan jaringan ginjal pada
pemeriksaan histopatologi jaringan. Peningkatan dosis EETM dari dosis 100
mg/kg bb, 200 mg/kg bb, dan 400 mg/kg bb menunjukkan peningkatan aktivitas
neproprotektif.
Kata kunci :
temu mangga (Curcuma mangga Val), serum kreatinin, histologi
ginjal, ginjal, parasetamol
vii
Universitas Sumatera Utara
NEPHROPROTECTIVE ACTIVITY ETHANOL EXTRACT OF MANGO
GINGER (Curcuma mangga Val) IN PARACETAMOL-INDUCED MALE
MICE
ABSTRACT
Mango ginger contains antioxidants that can counteract free radicals and
reduce the formation of N-acetyl - parabenzoquinoneimine (NAPQI) resulting
from the metabolism of paracetamol. This study aimed to identify whether the
ethanol extract of mango ginger (EEMG) has nephroprotective activity that can
decrease serum creatinine levels and protect kidney cell from necrosis caused by
paracetamol to male mice.
The EEMG activity was evaluated using normal control group, negative
control group, positive control group and EEMG with dose 100 mg/kg of body
weight, 200 mg/kg of body weigh, 400 mg/kg of body weight. Normal control and
negative control groups were given CMC Na 0.5% for 7 days. Mice positive
control group was given Curcuma® 58 mg/kg of body weight for 7 days. The
EEMG was given orally in mice at doses of 100 mg/kg of body weight, 200
mg/kg of body weight, and 400 mg/kg of body weight for 7 days. For negative
control group, positive control group, EEMG treatment group dose 100 mg/kg of
body weight, 200 mg/kg of body weight, 400 mg/kg of body weight, on day 7
after one hour of treatment, the mice was induced with paracetamol 1.05 g/kg of
body weight. Blood serum creatinine levels of all the groups were tested. The
kidneys of all experimental animal were transferred to 10% formalin solution for
histopathologic testing. Data were analyzed using One Way ANOVA test and
continued with Post Hoc Tuckey test.
Based on the test results, paracetamol administration caused renal demage
characterized by increased serum creatinine and histopathologic damage features.
Based on statistical analysis, EEMG with dose 100 mg/kg of body weight, 200
mg/kg of body weigh, 400 mg/kg of body weight were able to inhibit the increase
of creatinine levels and showed a significantly different ratio from negative
control (p = 0.000) and did not different significantly from positive control (p =
0.432). In the histopathology of renal cell dose 400 mg/kg of body weight did not
show any cell damage.
Mango ginger ethanolic extract doses of 400 mg/kg of body weight had
nephroprotective activity with serum creatinine = 0.5320 ± 0.0804 mg/dl, and at
dose 100 mg/kg of body weight, 200 mg/kg of body weight, 400 mg/kg of body
weight did not show renal tissue damage in tissue histophatologic examination.
Increased doses of EEMG from 100 mg/kg of body weight, 200 mg/kg of body
weight, and 400 mg/kg of body weight indicated increased neproprotective
activity.
Key words : mango ginger (Curcuma mango Val), serum creatinine, renal
histology, kidney, paracetamol
viii
Universitas Sumatera Utara
(Curcuma mangga Val) PADA MENCIT JANTAN YANG DIINDUKSI
PARASETAMOL
ABSTRAK
Temu mangga mengandung antioksidan yang dapat menangkal radikal
bebas dan mengurangi terbentuknya N-acetyl-parabenzoquinoneimine (NAPQI)
yang dihasilkan dari metabolisme parasetamol. Penelitian ini bertujuan untuk
mengidentifikasi apakah ekstrak etanol temu mangga memiliki aktivitas
nefroprotektif yaitu dapat menurunkan kadar serum kreatinin dan melindungi sel
ginjal dari nekrosis yang disebabkan oleh parasetamol terhadap mencit jantan.
Aktivitas ekstrak dievaluasi menggunakan kelompok kontrol normal,
kelompok kontrol negatif, kelompok kontrol positif dan kelompok perlakuan
ekstrak etanol temu mangga (EETM) dengan dosis 100 mg/kg bb, 200 mg/kg bb,
dan 400 mg/kg bb. Mencit kelompok kontrol normal dan kontrol negatif diberikan
CMC Na 0,5% selama 7 hari. Mencit kelompok kontrol positif diberikan
Curcuma® 58 mg/kg bb selama 7 hari. Ekstrak akan diberikan secara oral pada
mencit dengan dosis 100 mg/kg bb, 200 mg/kg bb dan 400 mg/kg bb. EETM
diberikan selama 7 hari. Untuk kelompok kontrol negatif, kontrol positif,
kelompok perlakuan EETM 100 mg/kg bb, EETM 200 mg/kg bb, EETM 400
mg/kg bb, pada hari ke-7, setelah 1 jam perlakuan, mencit diinduksi dengan
parasetamol 1,05 g/kg bb. Serum kadar kreatinin darah semua kelompok diuji.
Semua ginjal dipindahkan ke larutan formalin 10% untuk diuji histopatologi. Data
dianalisis dengan menggunakan uji One-Way ANOVA dan dilanjutkan dengan uji
Post Hoc Tuckey.
Berdasarkan hasil pengujian, pemberian parasetamol menyebabkan
kerusakan ginjal ditandai dengan peningkatan serum kreatinin serta gambaran
kerusakan histopatologi. Berdasarkan hasil analisis statistik, pemberian EETM
dosis 100, 200, dan 400 mg/kg bb mampu menghambat peningkatan kadar
kreatinin dan menunjukkan perbandingan berbeda signifikan dengan kontrol
negatif (p = 0,000) dan tidak berbeda signifikan dibandingkan kontrol positif (p =
0,432). Pada gambaran histopatologi sel ginjal dosis 400 mg/kg bb tidak
menunjukkan adanya kerusakan sel.
EETM dosis 400 mg/kg bb mempunyai aktivitas nefroprotektif dengan
kadar serum kreatinin = 0,5320 ± 0,0804 mg/dl, dan pada dosis 100 mg/kg bb,
200 mg/kg bb, 400 mg/kg bb tidak menunjukkan kerusakan jaringan ginjal pada
pemeriksaan histopatologi jaringan. Peningkatan dosis EETM dari dosis 100
mg/kg bb, 200 mg/kg bb, dan 400 mg/kg bb menunjukkan peningkatan aktivitas
neproprotektif.
Kata kunci :
temu mangga (Curcuma mangga Val), serum kreatinin, histologi
ginjal, ginjal, parasetamol
vii
Universitas Sumatera Utara
NEPHROPROTECTIVE ACTIVITY ETHANOL EXTRACT OF MANGO
GINGER (Curcuma mangga Val) IN PARACETAMOL-INDUCED MALE
MICE
ABSTRACT
Mango ginger contains antioxidants that can counteract free radicals and
reduce the formation of N-acetyl - parabenzoquinoneimine (NAPQI) resulting
from the metabolism of paracetamol. This study aimed to identify whether the
ethanol extract of mango ginger (EEMG) has nephroprotective activity that can
decrease serum creatinine levels and protect kidney cell from necrosis caused by
paracetamol to male mice.
The EEMG activity was evaluated using normal control group, negative
control group, positive control group and EEMG with dose 100 mg/kg of body
weight, 200 mg/kg of body weigh, 400 mg/kg of body weight. Normal control and
negative control groups were given CMC Na 0.5% for 7 days. Mice positive
control group was given Curcuma® 58 mg/kg of body weight for 7 days. The
EEMG was given orally in mice at doses of 100 mg/kg of body weight, 200
mg/kg of body weight, and 400 mg/kg of body weight for 7 days. For negative
control group, positive control group, EEMG treatment group dose 100 mg/kg of
body weight, 200 mg/kg of body weight, 400 mg/kg of body weight, on day 7
after one hour of treatment, the mice was induced with paracetamol 1.05 g/kg of
body weight. Blood serum creatinine levels of all the groups were tested. The
kidneys of all experimental animal were transferred to 10% formalin solution for
histopathologic testing. Data were analyzed using One Way ANOVA test and
continued with Post Hoc Tuckey test.
Based on the test results, paracetamol administration caused renal demage
characterized by increased serum creatinine and histopathologic damage features.
Based on statistical analysis, EEMG with dose 100 mg/kg of body weight, 200
mg/kg of body weigh, 400 mg/kg of body weight were able to inhibit the increase
of creatinine levels and showed a significantly different ratio from negative
control (p = 0.000) and did not different significantly from positive control (p =
0.432). In the histopathology of renal cell dose 400 mg/kg of body weight did not
show any cell damage.
Mango ginger ethanolic extract doses of 400 mg/kg of body weight had
nephroprotective activity with serum creatinine = 0.5320 ± 0.0804 mg/dl, and at
dose 100 mg/kg of body weight, 200 mg/kg of body weight, 400 mg/kg of body
weight did not show renal tissue damage in tissue histophatologic examination.
Increased doses of EEMG from 100 mg/kg of body weight, 200 mg/kg of body
weight, and 400 mg/kg of body weight indicated increased neproprotective
activity.
Key words : mango ginger (Curcuma mango Val), serum creatinine, renal
histology, kidney, paracetamol
viii
Universitas Sumatera Utara